PTGER4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTGER4
Entrez Gene ID	282331
Full Name	prostaglandin E receptor 4
Synonyms	ep4
General protein information	Preferred Names prostaglandin E receptor 4 Names prostaglandin E2 receptor EP4 subtype PGE receptor, EP4 subtype PGE2 receptor EP4 subtype prostaglandin E receptor 4 (subtype EP4) prostanoid EP4 receptor
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221540.4	XP_005221597.1	prostaglandin E2 receptor EP4 subtype isoform X1
NM_174589.2	NP_777014.1	prostaglandin E2 receptor EP4 subtype
XM_005221540.3	XP_005221597.1	prostaglandin E2 receptor EP4 subtype isoform X1
XM_010816781.3	XP_010815083.1	prostaglandin E2 receptor EP4 subtype isoform X1
XM_010816781.2	XP_010815083.1	prostaglandin E2 receptor EP4 subtype isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PTGER4	NP_001009078.1
Rattus norvegicus (Norway rat)	Ptger4	NP_114465.3
Danio rerio (zebrafish)	ptger4b	NP_001121839.1
Canis lupus familiaris (dog)	PTGER4	NP_001003054.1
Bos taurus (cattle)	PTGER4	NP_777014.1
	PTGER4	XP_001085257.1
Homo sapiens (human)	PTGER4	NP_000949.1
Mus musculus (house mouse)	Ptger4	NP_001129551.1
Gallus gallus (chicken)	PTGER4	NP_001074972.1
Xenopus tropicalis (tropical clawed frog)	ptger4	NP_001120554.1

Gene Ontology
Bibliography

Related articles in PubMed

Prostanoid receptors EP2, EP4, and FP are regulated by estradiol in bovine oviductal smooth muscle.
Huang N, Liu B, Dong Z, Mao W, Zhang N, Li C, Cao J
Prostaglandins & other lipid mediators121(Pt B)170-5(2015 Sep)

Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells.
Kowalczyk-Zieba I, Boruszewska D, Sinderewicz E, Grycmacher K, Woclawek-Potocka I
Prostaglandins & other lipid mediators121(Pt B)218-26(2015 Sep)

Prostaglandin E? down-regulates the expression of CD25 on bovine T cells, and this effect is mediated through the EP4 receptor.
Ma?lanka T, Spodniewska A, Barski D, Jasiecka A, Zu?ka-Prot M, Zi??kowski H, Markiewicz W, Jaroszewski JJ
Veterinary immunology and immunopathology160(3-4)192-200(2014 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Exploring cumulus-oocyte-complex-oviductal cell interactions: gene profiling in the bovine oviduct.
Gabler C, Odau S, Muller K, Schon J, Bondzio A, Einspanier R
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society59 Suppl 929-42(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that estradiol up-regulates mRNA and protein expression of 3 prostanoid receptors in oviduct smooth muscle: EP2/PTGER2 (prostaglandin E receptor 2); EP4/PTGER4 (prostaglandin E receptor 4); and FP/PTGFR (prostaglandin F2alpha receptor).
Title: Prostanoid receptors EP2, EP4, and FP are regulated by estradiol in bovine oviductal smooth muscle.

Data suggest that lysophosphatidic acid (LPA) up-regulates expression of SLCO2A1 (prostaglandin [PG] transporter), PTGER2/PTGER4 (PG receptors EP2/EP4), and mPGES1/cPGES (microsomal/cytosolic PG E synthases) in luteal cells.
Title: Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells.

The PGE2-mediated down-regulation of CD25 expression on T cells is mediated via the EP4 receptor, although selective activation of the EP2 receptor up-regulates the CD25 expression on these cells.
Title: Prostaglandin E? down-regulates the expression of CD25 on bovine T cells, and this effect is mediated through the EP4 receptor.

Quantitative RT-PCR revealed significant higher expression of EP2 and EP4 in the pre-ovulatory phase compared with the luteal phase in the bovine oviduct
Title: Exploring cumulus-oocyte-complex-oviductal cell interactions: gene profiling in the bovine oviduct.

EP4 is undetectable in endometrium and myometrium during the estrous cycle
Title: Molecular cloning and characterization of bovine prostaglandin E2 receptors EP2 and EP4: expression and regulation in endometrium and myometrium during the estrous cycle and early pregnancy.

The following PTGER4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTGER4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227962	XM_005221540.4 Latest version!	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa227962	XM_010816781.3 Latest version!	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa42743	NM_174589.2 Latest version!	Bos taurus prostaglandin E receptor 4 (PTGER4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa30635	XM_005221540.3 Latest version!	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OBa30635	XM_010816781.2 Latest version!	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTGER4 ( XM_005221540.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221597.1 Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa227962
Clone ID Related Accession (Same CDS sequence)	XM_005221540.4 , XM_010816781.3
Accession Version	XM_005221540.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	prostaglandin E2 receptor EP4 subtype isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221540.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGAG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAACCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221597.1
CDS	630..2108
Translation	MTAPGTNASSSQASNPLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYLKGQWPGGHALCEYSTFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPSMGLGSSRLQYPATWCFIDWTTNVTAHAAFSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQQHHAAAAAVALAPTACRAPPAASSPALPRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFVNQLYRPQLEPVISKNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGPHCSDSRRTSSAVSGHSRSFLSRELKEISSTSQTLLYTPELSENGLGGGRNLLPGVPGVGLTQIDSTSLRTLRISETSDSSQGQDSESFLLVDEVGGSCRAGPAPKGSSLQVTFPSETLNLSEKCI

Target ORF information:

RefSeq Version	XM_005221540.4
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221540.4

ORF Insert Sequence:

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGAG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAACCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGER4 ( XM_010816781.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815083.1 Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa227962
Clone ID Related Accession (Same CDS sequence)	XM_005221540.4 , XM_010816781.3
Accession Version	XM_010816781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	prostaglandin E2 receptor EP4 subtype isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGAG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAACCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815083.1
CDS	602..2080
Translation	MTAPGTNASSSQASNPLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYLKGQWPGGHALCEYSTFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPSMGLGSSRLQYPATWCFIDWTTNVTAHAAFSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQQHHAAAAAVALAPTACRAPPAASSPALPRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFVNQLYRPQLEPVISKNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGPHCSDSRRTSSAVSGHSRSFLSRELKEISSTSQTLLYTPELSENGLGGGRNLLPGVPGVGLTQIDSTSLRTLRISETSDSSQGQDSESFLLVDEVGGSCRAGPAPKGSSLQVTFPSETLNLSEKCI

Target ORF information:

RefSeq Version	XM_010816781.3
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816781.3

ORF Insert Sequence:

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGAG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAACCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGER4 ( NM_174589.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777014.1 Bos taurus prostaglandin E receptor 4 (PTGER4), mRNA.

Price & Availability↑

CloneID	OBa42743
Clone ID Related Accession (Same CDS sequence)	NM_174589.2
Accession Version	NM_174589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Bos taurus(cattle)
Product	prostaglandin E2 receptor EP4 subtype
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF539403.1. On Jun 3, 2003 this sequence version replaced NM_174589.1. ##Evidence-Data-START## Transcript exon combination :: AF539403.1, CR553139.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGTCCATTC CCGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AATCGCGCAA GGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGCG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAGCCA GTCATCGGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAG CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCTAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777014.1
CDS	6..1484
Translation	MSIPGTNASSSQASNPLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYLKGQWPGGHALCEYSTFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPSMGLGSSRLQYPATWCFIDWTTNVTAHAAFSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQQHHAAAAAVALAPTACRAPPAASSPALPRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFVNQLYRPQLEPVIGKNPDLQAIRIASVSPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGPHCSDSRRTSSAVSGHSRSFLSRELKEISSTSQTLLYTPELSENGLGGGRNLLPGVPGVGLTQIDSTSLRTLRISETSDSSQGQDSESFLLVDEVGGSCRAGPAPKGSSLQVTFPSETLNLSEKCI

Target ORF information:

RefSeq Version	NM_174589.2
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin E receptor 4 (PTGER4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174589.2

ORF Insert Sequence:

ATGTCCATTC CCGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AATCGCGCAA GGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGCG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAGCCA GTCATCGGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAG CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCTAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGER4 ( XM_005221540.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221597.1 Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X1, mRNA.

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CloneID	OBa30635
Clone ID Related Accession (Same CDS sequence)	XM_005221540.3 , XM_010816781.2
Accession Version	XM_005221540.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prostaglandin E2 receptor EP4 subtype isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104514.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221540.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGCG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAGCCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221597.1
CDS	624..2102
Translation	MTAPGTNASSSQASNPLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYLKGQWPGGHALCEYSTFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPSMGLGSSRLQYPATWCFIDWTTNVTAHAAFSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQQHHAAAAAVALAPTACRAPPAASSPALPRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFVNQLYRPQLEPVISKNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGPHCSDSRRTSSAVSGHSRSFLSRELKEISSTSQTLLYTPELSENGLGGGRNLLPGVPGVGLTQIDSTSLRTLRISETSDSSQGQDSESFLLVDEVGGSCRAGPAPKGSSLQVTFPSETLNLSEKCI

Target ORF information:

RefSeq Version	XM_005221540.3
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221540.3

ORF Insert Sequence:

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGCG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAGCCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGER4 ( XM_010816781.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815083.1 Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30635
Clone ID Related Accession (Same CDS sequence)	XM_005221540.3 , XM_010816781.2
Accession Version	XM_010816781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prostaglandin E2 receptor EP4 subtype isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104514.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGCG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAGCCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815083.1
CDS	618..2096
Translation	MTAPGTNASSSQASNPLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYLKGQWPGGHALCEYSTFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKRLAGLTLFAVYASNVLFCALPSMGLGSSRLQYPATWCFIDWTTNVTAHAAFSYMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQQHHAAAAAVALAPTACRAPPAASSPALPRLSDFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFVNQLYRPQLEPVISKNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSRRERSGPHCSDSRRTSSAVSGHSRSFLSRELKEISSTSQTLLYTPELSENGLGGGRNLLPGVPGVGLTQIDSTSLRTLRISETSDSSQGQDSESFLLVDEVGGSCRAGPAPKGSSLQVTFPSETLNLSEKCI

Target ORF information:

RefSeq Version	XM_010816781.2
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin E receptor 4 (PTGER4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816781.2

ORF Insert Sequence:

ATGACCGCCC CTGGGACCAA TGCATCATCC TCCCAGGCCT CCAACCCACT GAACAGCCCA 
GTGACCATCC CGGCGGTGAT GTTCATCTTC GGGGTCGTGG GCAACCTGGT GGCCATCGTG 
GTGCTCTGTA AGTCGCGCAA AGAGCAGAAG GAGACAACTT TCTACACACT GGTATGCGGG 
CTGGCGGTCA CCGACCTGCT GGGTACGTTG CTGGTGAGCC CGGTGACCAT CGCCACCTAC 
TTGAAGGGCC AATGGCCCGG GGGCCACGCG CTGTGCGAAT ACAGCACCTT CATCCTGCTC 
TTCTTTGGCC TGTCGGGGCT CAGCATCATC TGTGCGATGA GTATTGAGCG CTACCTGGCC 
ATCAACCACG CCTACTTCTA CAGCCACTAC GTGGACAAGC GGCTGGCGGG GCTCACGCTC 
TTCGCGGTCT ACGCGTCCAA CGTGCTCTTC TGCGCGCTGC CCAGCATGGG CCTGGGCAGT 
TCGCGGCTGC AGTACCCGGC CACCTGGTGC TTCATCGACT GGACCACCAA CGTGACAGCG 
CACGCCGCCT TCTCCTACAT GTATGCGGGC TTCAGTTCCT TCCTCATCCT CGCCACCGTG 
CTCTGCAACG TGCTCGTGTG CGGCGCGCTG CTCCGCATGC ACCGCCAGTT CATGCGCCGC 
ACCTCGCTGG GCACCGAGCA GCAGCACCAC GCGGCCGCCG CCGCCGTCGC CTTGGCCCCG 
ACAGCCTGCC GGGCTCCCCC GGCCGCCTCC TCCCCCGCCC TGCCGCGCCT CAGCGACTTT 
CGCCGCCGCC GGAGCTTCCG CCGCATCGCG GGCGCCGAGA TCCAGATGGT CATCTTACTC 
ATCGCCACCT CTCTGGTGGT GCTCATCTGC TCCATCCCGC TTGTGGTGCG AGTGTTCGTC 
AACCAGTTAT ATCGGCCACA ACTGGAGCCA GTCATCAGCA AAAACCCTGA TTTGCAGGCC 
ATCCGAATTG CTTCTGTGAA CCCCATTCTG GACCCCTGGA TATACATCCT CCTGCGGAAG 
ACAGTGCTCA GTAAAGCCAT AGAGAAGATC AAATGCCTCT TCTGCCGCAT CGGCGGGTCG 
CGCCGGGAGC GTTCGGGGCC GCACTGCTCA GACAGCAGAA GGACGTCCTC CGCCGTGTCC 
GGCCACTCCC GCTCCTTCCT CTCCCGGGAG CTGAAAGAGA TCAGCAGCAC GTCGCAGACC 
CTCCTGTACA CGCCAGAACT CAGTGAAAAC GGCCTCGGGG GAGGCAGGAA TCTGCTGCCA 
GGTGTGCCCG GCGTGGGCCT GACCCAAATA GACAGCACGT CGCTTAGGAC TCTGCGAATA 
TCGGAGACCT CAGACTCCTC ACAGGGGCAG GACTCGGAGA GCTTCTTACT GGTGGACGAG 
GTTGGCGGGA GCTGCAGGGC TGGGCCCGCC CCCAAGGGGA GCTCCCTGCA AGTCACGTTT 
CCCAGTGAAA CGCTGAACTT ATCAGAAAAA TGTATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prostanoid receptors EP2, EP4, and FP are regulated by estradiol in bovine oviductal smooth muscle.
Prostaglandins & other lipid mediators121(Pt B)170-5(2015 Sep)
Huang N,Liu B,Dong Z,Mao W,Zhang N,Li C,Cao J

Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells.
Prostaglandins & other lipid mediators121(Pt B)218-26(2015 Sep)
Kowalczyk-Zieba I,Boruszewska D,Sinderewicz E,Grycmacher K,Woclawek-Potocka I

Prostaglandin E? down-regulates the expression of CD25 on bovine T cells, and this effect is mediated through the EP4 receptor.
Veterinary immunology and immunopathology160(3-4)192-200(2014 Aug)
Ma?lanka T,Spodniewska A,Barski D,Jasiecka A,Zu?ka-Prot M,Zi??kowski H,Markiewicz W,Jaroszewski JJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Exploring cumulus-oocyte-complex-oviductal cell interactions: gene profiling in the bovine oviduct.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society59 Suppl 929-42(2008 Dec)
Gabler C,Odau S,Muller K,Schon J,Bondzio A,Einspanier R

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PTGER4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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