PRKACA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKACA
Entrez Gene ID	282322
Full Name	protein kinase cAMP-activated catalytic subunit alpha
General protein information	Preferred Names protein kinase cAMP-activated catalytic subunit alpha Names cAMP-dependent protein kinase catalytic subunit alpha PKA C-alpha protein kinase, cAMP-dependent, catalytic, alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174584.2	NP_777009.1	cAMP-dependent protein kinase catalytic subunit alpha

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkaca	NP_032880.1
Danio rerio (zebrafish)	prkacab	NP_001003470.1
Kluyveromyces lactis	KLLA0D03190g	XP_453207.1
Homo sapiens (human)	PRKACA	NP_002721.1
Bos taurus (cattle)	PRKACA	NP_777009.1
Schizosaccharomyces pombe (fission yeast)	pka1	NP_595159.1
Xenopus tropicalis (tropical clawed frog)	prkaca	NP_001016403.1
Pan troglodytes (chimpanzee)	PRKACA	XP_001171437.1
Saccharomyces cerevisiae (baker's yeast)	TPK2	NP_015121.1
Eremothecium gossypii	AGOS_AFL090W	NP_985458.1
Rattus norvegicus (Norway rat)	Prkaca	NP_001094392.1
Canis lupus familiaris (dog)	PRKACA	NP_001003032.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytoplasmic kinases downstream of GPR30 suppress gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone secretion from bovine anterior pituitary cells.
Rudolf FO, Kadokawa H
The Journal of reproduction and development62(1)65-9(2016)

Probes of the mitochondrial cAMP-dependent protein kinase.
Shell JR, Lawrence DS
Biochimica et biophysica acta1834(7)1359-63(2013 Jul)

Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase.
M?llner C, Steinecker B, Gorischek A, Schreibmayer W
The FEBS journal276(21)6218-26(2009 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Signaling the signal, cyclic AMP-dependent protein kinase inhibition by insulin-formed H2O2 and reactivation by thioredoxin.
de Pi?a MZ, V?zquez-Meza H, Pardo JP, Rend?n JL, Villalobos-Molina R, Riveros-Rosas H, Pi?a E
The Journal of biological chemistry283(18)12373-86(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PKA and MEK (thus, also pERK) are the intracellular mediators downstream of GPR30 that induce the non-genomic suppression of GnRH-induced LH secretion from bovine AP cells by estradiol or G1
Title: Cytoplasmic kinases downstream of GPR30 suppress gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone secretion from bovine anterior pituitary cells.

Data indicate that mitochondrial cAMP-dependent protein kinase (PKA) activity is regulated by the protease calpain.
Title: Probes of the mitochondrial cAMP-dependent protein kinase.

These data strongly suggest that H(2)O(2) promotes the formation of an intersubunit disulfide bond, impairing cAMP-dependent PKA activation.
Title: Signaling the signal, cyclic AMP-dependent protein kinase inhibition by insulin-formed H2O2 and reactivation by thioredoxin.

Purification and characterisation of protein kinase catalytic subunit (PKAcat) from bovine lens.
Title: Protein kinase catalytic subunit (PKAcat) from bovine lens: purification, characterization and phosphorylation of lens crystallins.

structural basis of selectivity for protein kinase A in complex with Rho-kinase inhibitors by x-ray crystallography
Title: Protein kinase A in complex with Rho-kinase inhibitors Y-27632, Fasudil, and H-1152P: structural basis of selectivity.

The following PRKACA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKACA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43601	NM_174584.2 Latest version!	Bos taurus protein kinase cAMP-activated catalytic subunit alpha (PRKACA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKACA ( NM_174584.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777009.1 Bos taurus protein kinase cAMP-activated catalytic subunit alpha (PRKACA), mRNA.

Price & Availability↑

CloneID	OBa43601
Clone ID Related Accession (Same CDS sequence)	NM_174584.2
Accession Version	NM_174584.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	cAMP-dependent protein kinase catalytic subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X67154.1. On Jun 3, 2003 this sequence version replaced NM_174584.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X67154.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAAG AAAGCGTGAA AGAGTTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAATC CTGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTG GGTACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAGCACA TGGAGACCGG GAACCACTAC GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAGCTGA AACAGATTGA GCACACCCTG AATGAGAAGC GCATCCTGCA GGCGGTCAAC 
TTTCCGTTCC TTGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAATTT ATACATGGTC 
ATGGAGTACG TGCCCGGTGG GGAGATGTTC TCACACCTGC GACGGATCGG GAGGTTCAGT 
GAGCCCCACG CACGCTTCTA CGCCGCCCAG ATTGTCCTGA CCTTTGAGTA CCTGCACTCG 
CTTGATCTCA TTTACCGGGA CCTGAAGCCA GAGAACCTCC TCATCGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTTGG TTTCGCCAAG CGTGTGAAAG GCCGCACCTG GACCTTGTGT 
GGGACCCCTG AGTACTTGGC CCCCGAGATC ATCCTGAGTA AAGGCTACAA CAAAGCTGTG 
GACTGGTGGG CCCTGGGGGT CCTCATCTAC GAAATGGCCG CAGGCTACCC GCCCTTCTTT 
GCCGACCAGC CCATCCAGAT CTACGAGAAG ATTGTCTCTG GGAAGGTGCG GTTTCCATCC 
CACTTCAGCT CTGACTTGAA GGATCTGCTG CGCAACCTCC TACAAGTGGA CCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAT GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ACACAAGTAA CTTTGACGAC TATGAGGAGG AAGAGATCCG AGTCTCTATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777009.1
CDS	2..1057
Translation	MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWENPAQNTAHLDQFERIKTLGTGSFGRVMLVKHMETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Target ORF information:

RefSeq Version	NM_174584.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cAMP-activated catalytic subunit alpha (PRKACA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174584.2

ORF Insert Sequence:

ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAAG AAAGCGTGAA AGAGTTCTTA 
GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAATC CTGCTCAGAA CACAGCCCAC 
TTGGATCAGT TTGAACGAAT CAAGACCCTG GGTACGGGCT CCTTCGGGCG GGTGATGCTG 
GTGAAGCACA TGGAGACCGG GAACCACTAC GCCATGAAGA TCCTCGACAA ACAGAAGGTG 
GTGAAGCTGA AACAGATTGA GCACACCCTG AATGAGAAGC GCATCCTGCA GGCGGTCAAC 
TTTCCGTTCC TTGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAATTT ATACATGGTC 
ATGGAGTACG TGCCCGGTGG GGAGATGTTC TCACACCTGC GACGGATCGG GAGGTTCAGT 
GAGCCCCACG CACGCTTCTA CGCCGCCCAG ATTGTCCTGA CCTTTGAGTA CCTGCACTCG 
CTTGATCTCA TTTACCGGGA CCTGAAGCCA GAGAACCTCC TCATCGACCA GCAGGGCTAC 
ATTCAGGTGA CAGACTTTGG TTTCGCCAAG CGTGTGAAAG GCCGCACCTG GACCTTGTGT 
GGGACCCCTG AGTACTTGGC CCCCGAGATC ATCCTGAGTA AAGGCTACAA CAAAGCTGTG 
GACTGGTGGG CCCTGGGGGT CCTCATCTAC GAAATGGCCG CAGGCTACCC GCCCTTCTTT 
GCCGACCAGC CCATCCAGAT CTACGAGAAG ATTGTCTCTG GGAAGGTGCG GTTTCCATCC 
CACTTCAGCT CTGACTTGAA GGATCTGCTG CGCAACCTCC TACAAGTGGA CCTCACCAAG 
CGCTTTGGGA ACCTCAAGAA TGGGGTCAAT GATATCAAGA ACCACAAGTG GTTTGCCACA 
ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA 
GGCCCTGGGG ACACAAGTAA CTTTGACGAC TATGAGGAGG AAGAGATCCG AGTCTCTATC 
AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytoplasmic kinases downstream of GPR30 suppress gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone secretion from bovine anterior pituitary cells.
The Journal of reproduction and development62(1)65-9(2016)
Rudolf FO,Kadokawa H

Probes of the mitochondrial cAMP-dependent protein kinase.
Biochimica et biophysica acta1834(7)1359-63(2013 Jul)
Shell JR,Lawrence DS

Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase.
The FEBS journal276(21)6218-26(2009 Nov)
M?llner C,Steinecker B,Gorischek A,Schreibmayer W

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Signaling the signal, cyclic AMP-dependent protein kinase inhibition by insulin-formed H2O2 and reactivation by thioredoxin.
The Journal of biological chemistry283(18)12373-86(2008 May)
de Pi?a MZ,V?zquez-Meza H,Pardo JP,Rend?n JL,Villalobos-Molina R,Riveros-Rosas H,Pi?a E

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PRKACA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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