PIK3CA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIK3CA
Entrez Gene ID	282306
Full Name	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
General protein information	Preferred Names phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Names phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform PI3-kinase p110 subunit alpha PI3-kinase subunit alpha PI3K PI3K-alpha PI3Kalpha p110alpha phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha phosphoinositide-3-kinase, catalytic, alpha polypeptide ptdIns-3-kinase p110 ptdIns-3-kinase subunit alpha ptdIns-3-kinase subunit p110-alpha serine/threonine protein kinase PIK3CA
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024993013.1	XP_024848781.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform isoform X1
NM_174574.1	NP_776999.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_024993044.1	XP_024848812.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC561737	XP_690235.2
Xenopus tropicalis (tropical clawed frog)	pik3ca	NP_001119972.1
Canis lupus familiaris (dog)	PIK3CA	XP_545208.2
	PIK3CA	XP_001109162.1
Bos taurus (cattle)	PIK3CA	NP_776999.1
Mus musculus (house mouse)	Pik3ca	NP_032865.2
Rattus norvegicus (Norway rat)	Pik3ca	NP_596890.2
Pan troglodytes (chimpanzee)	PIK3CA	XP_001167506.1
Danio rerio (zebrafish)	LOC100334726	XP_005174483.1
Homo sapiens (human)	PIK3CA	NP_006209.2
Gallus gallus (chicken)	PIK3CA	NP_001004410.1
Caenorhabditis elegans (roundworm)	age-1	NP_496462.2

Gene Ontology
Bibliography

Related articles in PubMed

Class I Phosphoinositide 3-Kinase Exerts a Differential Role on Cell Survival and Cell Trafficking in Retina.
Azadi S, Brush RS, Anderson RE, Rajala RV
Advances in experimental medicine and biology854363-9(2016)

Critical evaluation of the two-state model describing the equilibrium unfolding of the PI3K SH3 domain by time-resolved fluorescence resonance energy transfer.
Kishore M, Krishnamoorthy G, Udgaonkar JB
Biochemistry52(52)9482-96(2013 Dec)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit.
Miled N, Yan Y, Hon WC, Perisic O, Zvelebil M, Inbar Y, Schneidman-Duhovny D, Wolfson HJ, Backer JM, Williams RL
Science (New York, N.Y.)317(5835)239-42(2007 Jul)

Phosphatidylinositol 3-kinase C2alpha is essential for ATP-dependent priming of neurosecretory granule exocytosis.
Meunier FA, Osborne SL, Hammond GR, Cooke FT, Parker PJ, Domin J, Schiavo G
Molecular biology of the cell16(10)4841-51(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that both class I and III phosphoinositide 3-kinases (PI3Ks) re present in trafficking vesicles.
Title: Class I Phosphoinositide 3-Kinase Exerts a Differential Role on Cell Survival and Cell Trafficking in Retina.

There are multiple conformations in equilibrium during the course of PI3K SH3 domain unfolding.
Title: Critical evaluation of the two-state model describing the equilibrium unfolding of the PI3K SH3 domain by time-resolved fluorescence resonance energy transfer.

crystallographic and biochemical approaches used to gain insight into activating mutations in two noncatalytic p110alpha domains-the adaptor-binding and the helical domains
Title: Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit.

Production of PtdIns3P by PI3K-C2alpha is required for acquisition of fusion competence in neurosecretion.
Title: Phosphatidylinositol 3-kinase C2alpha is essential for ATP-dependent priming of neurosecretory granule exocytosis.

PI3K has a role in activation of 5'-AMP-activated kinase during hypoxia-reoxygenation of bovine aortic endothelial cells
Title: Activation of 5'-AMP-activated kinase is mediated through c-Src and phosphoinositide 3-kinase activity during hypoxia-reoxygenation of bovine aortic endothelial cells. Role of peroxynitrite.

The following PIK3CA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIK3CA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01285	NM_174574.1 Latest version!	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa01285	XM_024993013.1 Latest version!	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa01285	XM_024993044.1 Latest version!	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIK3CA ( NM_174574.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776999.1 Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), mRNA.

Price & Availability↑

CloneID	OBa01285
Clone ID Related Accession (Same CDS sequence)	XM_024993013.1 , NM_174574.1 , XM_024993044.1
Accession Version	NM_174574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-01
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M93252.1. On Mar 29, 2005 this sequence version replaced XM_613857.1. ##Evidence-Data-START## Transcript exon combination :: M93252.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCCAA GACCATCATC AGGTGAACTG TGGGGCATCC ACTTGATGCC CCCAAGAATC 
CTAGTAGAAT GTTTACTACC AAATGGGATG ATAGTGACTT TAGAATGCCT CCGTGAGGCT 
ACGTTAATAA CGATAAAGCA TGAACTATTT AAAGAAGCAA GAAAATACCC TCTCCATCAA 
CTTCTTCAAG ATGAATCTTC TTACATTTTC GTAAGTGTTA CCCAAGAAGC AGAAAGGGAA 
GAATTTTTTG ATGAAACAAG ACGACTTTGT GACCTTCGGC TTTTTCAACC CTTTTTAAAA 
GTAATTGAAC CAGTAGGCAA CCGTGAAGAA AAGATCCTCA ATCGAGAAAT TGGTTTTGCT 
ATCGGCATGC CAGTGTGTGA ATTCGATATG GTTAAAGATC CAGAAGTACA GGACTTCCGA 
AGAAATATTC TCAATGTTTG TAAAGAAGCT GTGGATCTTA GGGATCTTAA TTCACCTCAT 
AGTAGAGCAA TGTATGTTTA TCCTCCAAAT GTAGAATCTT CACCAGAACT GCCAAAGCAC 
ATATATAATA AATTGGATAA AGGGCAAATA ATAGTGGTGA TTTGGGTAAT AGTTTCTCCA 
AATAATGACA AACAGAAGTA TACTCTGAAA ATCAACCATG ACTGTGTGCC AGAACAAGTA 
ATTGCTGAAG CAATCAGGAA AAAAACTCGA AGTATGTTGC TATCATCTGA ACAACTAAAA 
CTCTGTGTTT TAGAATATCA GGGCAAGTAT ATTTTAAAAG TGTGTGGATG TGATGAATAC 
TTCCTAGAAA AATATCCTCT GAGTCAGTAT AAGTATATAA GAAGCTGTAT AATGCTTGGG 
AGGATGCCCA ATTTGATGCT GATGGCTAAA GAAAGCCTCT ATTCTCAACT GCCAATGGAC 
TGTTTTACAA TGCCATCATA TTCCAGACGC ATCTCCACAG CTACGCCATA TATGAATGGA 
GAAACATCTA CAAAATCCCT TTGGGTTATA AATAGTGCAC TCAGAATAAA AATTCTTTGT 
GCAACCTATG TGAATGTAAA TATTCGAGAC ATTGACAAGA TTTATGTTCG AACAGGTATC 
TACCATGGAG GAGAACCCTT ATGTGATAAT GTGAACACTC AAAGAGTACC TTGTTCCAAT 
CCCAGGTGGA ATGAATGGCT GAATTACGAT ATATACATTC CTGATCTTCC TCGTGCTGCT 
CGACTTTGCC TTTCCATTTG TTCTGTTAAA GGCCGAAAGG GTGCTAAAGA GGAACACTGT 
CCATTGGCCT GGGGAAATAT AAACTTGTTT GATTACACAG ATACTCTAGT ATCTGGAAAA 
ATGGCTTTGA ATCTTTGGCC AGTACCTCAT GGACTAGAAG ATTTGCTGAA CCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAAGAAACT CCATGTTTAG AGTTGGAGTT TGACTGGTTC 
AGCAGTGTGG TAAAGTTTCC AGATATGTCA GTGATTGAAG AGCATGCCAA TTGGTCTGTA 
TCCCGTGAAG CAGGATTTAG TTATTCCCAT GCAGGACTGA GTAACAGACT AGCTAGAGAC 
AATGAATTAA GAGAAAATGA TAAAGAACAG CTCCGAGCAA TTTGTACACG AGATCCTCTA 
TCTGAAATCA CTGAGCAAGA GAAAGATTTT CTGTGGAGCC ACAGACACTA TTGTGTAACT 
ATCCCCGAAA TTCTACCCAA ATTGCTTCTG TCTGTTAAAT GGAACTCTAG AGATGAAGTA 
GCTCAGATGT ACTGCTTGGT AAAAGATTGG CCTCCAATCA AGCCTGAACA GGCTATGGAG 
CTTCTGGACT GCAATTACCC AGATCCTATG GTTCGAGGTT TTGCTGTTCG GTGCTTAGAA 
AAATATTTAA CAGATGACAA ACTTTCTCAG TACCTAATTC AGCTAGTACA GGTACTAAAA 
TATGAACAGT ATTTGGATAA CCTGCTTGTG AGATTTTTAC TCAAAAAAGC GTTAACTAAT 
CAAAGGATCG GTCACTTTTT CTTTTGGCAT TTAAAATCTG AGATGCACAA TAAAACAGTT 
AGTCAGAGGT TTGGCCTGCT TTTGGAGTCC TATTGCCGTG CATGTGGGAT GTATCTGAAG 
CACCTTAATA GGCAAGTTGA GGCTATGGAA AAGCTCATTA ACTTGACTGA CATTCTCAAA 
CAAGAGAAGA AGGATGAAAC ACAAAAGGTA CAGATGAAGT TTTTAGTTGA GCAAATGCGG 
CGACCAGATT TCATGGATGC TCTCCAGGGC TTTCTGTCTC CTCTAAACCC TGCTCATCAG 
CTGGGAAATC TCAGGCTTGA AGAGTGTCGA ATTATGTCTT CTGCAAAAAG GCCACTGTGG 
TTGAATTGGG AGAACCCAGA CATCATGTCA GAATTACTCT TTCAGAACAA TGAGATCATC 
TTTAAAAATG GGGATGATTT ACGGCAAGAT ATGCTAACCC TTCAGATTAT TCGCATTATG 
GAAAATATCT GGCAAAATCA AGGTCTTGAT CTTCGAATGT TACCTTATGG ATGTCTGTCA 
ATCGGTGACT GTGTGGGACT TATCGAGGTG GTGAGAAATT CTCACACTAT AATGCAGATT 
CAGTGTAAAG GAGGCCTGAA AGGTGCACTG CAGTTTAACA GCCACACACT CCATCAGTGG 
CTCAAAGACA AGAACAAGGG GGAAATATAT GATGCGGCCA TCGATTTGTT TACACGATCA 
TGTGCTGGAT ATTGTGTTGC CACCTTCATT TTGGGAATTG GAGATCGTCA CAATAGTAAT 
ATCATGGTTA AAGATGATGG ACAACTGTTT CATATAGATT TTGGACACTT TTTGGATCAC 
AAGAAGAAAA AATTTGGTTA TAAACGAGAG CGCGTGCCGT TTGTTTTGAC ACAAGATTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGCACAAAGA CAAGAGAATT TGAGAGGTTT 
CAGGAGATGT GTTACAAGGC TTATCTAGCT ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCAA TGATGCTTGG CTCTGGAATG CCAGAACTGC AATCTTTTGA TGATATTGCA 
TACATTCGAA AGACCCTAGC TTTAGATAAA ACTGAGCAAG AGGCTTTGGA GTATTTCATG 
AAACAAATGA ATGATGCACA CCATGGTGGC TGGACAACAA AAATGGATTG GATCTTCCAC 
ACAATTAAGC AGCATGCTTT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776999.1
CDS	1..3207
Translation	MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLRAICTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN

Target ORF information:

RefSeq Version	NM_174574.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174574.1

ORF Insert Sequence:

ATGCCTCCAA GACCATCATC AGGTGAACTG TGGGGCATCC ACTTGATGCC CCCAAGAATC 
CTAGTAGAAT GTTTACTACC AAATGGGATG ATAGTGACTT TAGAATGCCT CCGTGAGGCT 
ACGTTAATAA CGATAAAGCA TGAACTATTT AAAGAAGCAA GAAAATACCC TCTCCATCAA 
CTTCTTCAAG ATGAATCTTC TTACATTTTC GTAAGTGTTA CCCAAGAAGC AGAAAGGGAA 
GAATTTTTTG ATGAAACAAG ACGACTTTGT GACCTTCGGC TTTTTCAACC CTTTTTAAAA 
GTAATTGAAC CAGTAGGCAA CCGTGAAGAA AAGATCCTCA ATCGAGAAAT TGGTTTTGCT 
ATCGGCATGC CAGTGTGTGA ATTCGATATG GTTAAAGATC CAGAAGTACA GGACTTCCGA 
AGAAATATTC TCAATGTTTG TAAAGAAGCT GTGGATCTTA GGGATCTTAA TTCACCTCAT 
AGTAGAGCAA TGTATGTTTA TCCTCCAAAT GTAGAATCTT CACCAGAACT GCCAAAGCAC 
ATATATAATA AATTGGATAA AGGGCAAATA ATAGTGGTGA TTTGGGTAAT AGTTTCTCCA 
AATAATGACA AACAGAAGTA TACTCTGAAA ATCAACCATG ACTGTGTGCC AGAACAAGTA 
ATTGCTGAAG CAATCAGGAA AAAAACTCGA AGTATGTTGC TATCATCTGA ACAACTAAAA 
CTCTGTGTTT TAGAATATCA GGGCAAGTAT ATTTTAAAAG TGTGTGGATG TGATGAATAC 
TTCCTAGAAA AATATCCTCT GAGTCAGTAT AAGTATATAA GAAGCTGTAT AATGCTTGGG 
AGGATGCCCA ATTTGATGCT GATGGCTAAA GAAAGCCTCT ATTCTCAACT GCCAATGGAC 
TGTTTTACAA TGCCATCATA TTCCAGACGC ATCTCCACAG CTACGCCATA TATGAATGGA 
GAAACATCTA CAAAATCCCT TTGGGTTATA AATAGTGCAC TCAGAATAAA AATTCTTTGT 
GCAACCTATG TGAATGTAAA TATTCGAGAC ATTGACAAGA TTTATGTTCG AACAGGTATC 
TACCATGGAG GAGAACCCTT ATGTGATAAT GTGAACACTC AAAGAGTACC TTGTTCCAAT 
CCCAGGTGGA ATGAATGGCT GAATTACGAT ATATACATTC CTGATCTTCC TCGTGCTGCT 
CGACTTTGCC TTTCCATTTG TTCTGTTAAA GGCCGAAAGG GTGCTAAAGA GGAACACTGT 
CCATTGGCCT GGGGAAATAT AAACTTGTTT GATTACACAG ATACTCTAGT ATCTGGAAAA 
ATGGCTTTGA ATCTTTGGCC AGTACCTCAT GGACTAGAAG ATTTGCTGAA CCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAAGAAACT CCATGTTTAG AGTTGGAGTT TGACTGGTTC 
AGCAGTGTGG TAAAGTTTCC AGATATGTCA GTGATTGAAG AGCATGCCAA TTGGTCTGTA 
TCCCGTGAAG CAGGATTTAG TTATTCCCAT GCAGGACTGA GTAACAGACT AGCTAGAGAC 
AATGAATTAA GAGAAAATGA TAAAGAACAG CTCCGAGCAA TTTGTACACG AGATCCTCTA 
TCTGAAATCA CTGAGCAAGA GAAAGATTTT CTGTGGAGCC ACAGACACTA TTGTGTAACT 
ATCCCCGAAA TTCTACCCAA ATTGCTTCTG TCTGTTAAAT GGAACTCTAG AGATGAAGTA 
GCTCAGATGT ACTGCTTGGT AAAAGATTGG CCTCCAATCA AGCCTGAACA GGCTATGGAG 
CTTCTGGACT GCAATTACCC AGATCCTATG GTTCGAGGTT TTGCTGTTCG GTGCTTAGAA 
AAATATTTAA CAGATGACAA ACTTTCTCAG TACCTAATTC AGCTAGTACA GGTACTAAAA 
TATGAACAGT ATTTGGATAA CCTGCTTGTG AGATTTTTAC TCAAAAAAGC GTTAACTAAT 
CAAAGGATCG GTCACTTTTT CTTTTGGCAT TTAAAATCTG AGATGCACAA TAAAACAGTT 
AGTCAGAGGT TTGGCCTGCT TTTGGAGTCC TATTGCCGTG CATGTGGGAT GTATCTGAAG 
CACCTTAATA GGCAAGTTGA GGCTATGGAA AAGCTCATTA ACTTGACTGA CATTCTCAAA 
CAAGAGAAGA AGGATGAAAC ACAAAAGGTA CAGATGAAGT TTTTAGTTGA GCAAATGCGG 
CGACCAGATT TCATGGATGC TCTCCAGGGC TTTCTGTCTC CTCTAAACCC TGCTCATCAG 
CTGGGAAATC TCAGGCTTGA AGAGTGTCGA ATTATGTCTT CTGCAAAAAG GCCACTGTGG 
TTGAATTGGG AGAACCCAGA CATCATGTCA GAATTACTCT TTCAGAACAA TGAGATCATC 
TTTAAAAATG GGGATGATTT ACGGCAAGAT ATGCTAACCC TTCAGATTAT TCGCATTATG 
GAAAATATCT GGCAAAATCA AGGTCTTGAT CTTCGAATGT TACCTTATGG ATGTCTGTCA 
ATCGGTGACT GTGTGGGACT TATCGAGGTG GTGAGAAATT CTCACACTAT AATGCAGATT 
CAGTGTAAAG GAGGCCTGAA AGGTGCACTG CAGTTTAACA GCCACACACT CCATCAGTGG 
CTCAAAGACA AGAACAAGGG GGAAATATAT GATGCGGCCA TCGATTTGTT TACACGATCA 
TGTGCTGGAT ATTGTGTTGC CACCTTCATT TTGGGAATTG GAGATCGTCA CAATAGTAAT 
ATCATGGTTA AAGATGATGG ACAACTGTTT CATATAGATT TTGGACACTT TTTGGATCAC 
AAGAAGAAAA AATTTGGTTA TAAACGAGAG CGCGTGCCGT TTGTTTTGAC ACAAGATTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGCACAAAGA CAAGAGAATT TGAGAGGTTT 
CAGGAGATGT GTTACAAGGC TTATCTAGCT ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCAA TGATGCTTGG CTCTGGAATG CCAGAACTGC AATCTTTTGA TGATATTGCA 
TACATTCGAA AGACCCTAGC TTTAGATAAA ACTGAGCAAG AGGCTTTGGA GTATTTCATG 
AAACAAATGA ATGATGCACA CCATGGTGGC TGGACAACAA AAATGGATTG GATCTTCCAC 
ACAATTAAGC AGCATGCTTT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_024993013.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848781.1 Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa01285
Clone ID Related Accession (Same CDS sequence)	XM_024993013.1 , NM_174574.1 , XM_024993044.1
Accession Version	XM_024993013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCATCATC AGGTGAACTG TGGGGCATCC ACTTGATGCC CCCAAGAATC 
CTAGTAGAAT GTTTACTACC AAATGGGATG ATAGTGACTT TAGAATGCCT CCGTGAGGCT 
ACGTTAATAA CGATAAAGCA TGAACTATTT AAAGAAGCAA GAAAATACCC TCTCCATCAA 
CTTCTTCAAG ATGAATCTTC TTACATTTTC GTAAGTGTTA CCCAAGAAGC AGAAAGGGAA 
GAATTTTTTG ATGAAACAAG ACGACTTTGT GACCTTCGGC TTTTTCAACC CTTTTTAAAA 
GTAATTGAAC CAGTAGGCAA CCGTGAAGAA AAGATCCTCA ATCGAGAAAT TGGTTTTGCT 
ATCGGCATGC CAGTGTGTGA ATTCGATATG GTTAAAGATC CAGAAGTACA GGACTTCCGA 
AGAAATATTC TCAATGTTTG TAAAGAAGCT GTGGATCTTA GGGATCTTAA TTCACCTCAT 
AGTAGAGCAA TGTATGTTTA TCCTCCAAAT GTAGAATCTT CACCAGAACT GCCAAAGCAC 
ATATATAATA AATTGGATAA AGGGCAAATA ATAGTGGTGA TTTGGGTAAT AGTTTCTCCA 
AATAATGACA AACAGAAGTA TACTCTGAAA ATCAACCATG ACTGTGTGCC AGAACAAGTA 
ATTGCTGAAG CAATCAGGAA AAAAACTCGA AGTATGTTGC TATCATCTGA ACAACTAAAA 
CTCTGTGTTT TAGAATATCA GGGCAAGTAT ATTTTAAAAG TGTGTGGATG TGATGAATAC 
TTCCTAGAAA AATATCCTCT GAGTCAGTAT AAGTATATAA GAAGCTGTAT AATGCTTGGG 
AGGATGCCCA ATTTGATGCT GATGGCTAAA GAAAGCCTCT ATTCTCAACT GCCAATGGAC 
TGTTTTACAA TGCCATCATA TTCCAGACGC ATCTCCACAG CTACGCCATA TATGAATGGA 
GAAACATCTA CAAAATCCCT TTGGGTTATA AATAGTGCAC TCAGAATAAA AATTCTTTGT 
GCAACCTATG TGAATGTAAA TATTCGAGAC ATTGACAAGA TTTATGTTCG AACAGGTATC 
TACCATGGAG GAGAACCCTT ATGTGATAAT GTGAACACTC AAAGAGTACC TTGTTCCAAT 
CCCAGGTGGA ATGAATGGCT GAATTACGAT ATATACATTC CTGATCTTCC TCGTGCTGCT 
CGACTTTGCC TTTCCATTTG TTCTGTTAAA GGCCGAAAGG GTGCTAAAGA GGAACACTGT 
CCATTGGCCT GGGGAAATAT AAACTTGTTT GATTACACAG ATACTCTAGT ATCTGGAAAA 
ATGGCTTTGA ATCTTTGGCC AGTACCTCAT GGACTAGAAG ATTTGCTGAA CCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAAGAAACT CCATGTTTAG AGTTGGAGTT TGACTGGTTC 
AGCAGTGTGG TAAAGTTTCC AGATATGTCA GTGATTGAAG AGCATGCCAA TTGGTCTGTA 
TCCCGTGAAG CAGGATTTAG TTATTCCCAT GCAGGACTGA GTAACAGACT AGCTAGAGAC 
AATGAATTAA GAGAAAATGA TAAAGAACAG CTCCGAGCAA TTTGTACACG AGATCCTCTA 
TCTGAAATCA CTGAGCAAGA GAAAGATTTT CTGTGGAGCC ACAGACACTA TTGTGTAACT 
ATCCCCGAAA TTCTACCCAA ATTGCTTCTG TCTGTTAAAT GGAACTCTAG AGATGAAGTA 
GCTCAGATGT ACTGCTTGGT AAAAGATTGG CCTCCAATCA AGCCTGAACA GGCTATGGAG 
CTTCTGGACT GCAATTACCC AGATCCTATG GTTCGAGGTT TTGCTGTTCG GTGCTTAGAA 
AAATATTTAA CAGATGACAA ACTTTCTCAG TACCTAATTC AGCTAGTACA GGTACTAAAA 
TATGAACAGT ATTTGGATAA CCTGCTTGTG AGATTTTTAC TCAAAAAAGC GTTAACTAAT 
CAAAGGATCG GTCACTTTTT CTTTTGGCAT TTAAAATCTG AGATGCACAA TAAAACAGTT 
AGTCAGAGGT TTGGCCTGCT TTTGGAGTCC TATTGCCGTG CATGTGGGAT GTATCTGAAG 
CACCTTAATA GGCAAGTTGA GGCTATGGAA AAGCTCATTA ACTTGACTGA CATTCTCAAA 
CAAGAGAAGA AGGATGAAAC ACAAAAGGTA CAGATGAAGT TTTTAGTTGA GCAAATGCGG 
CGACCAGATT TCATGGATGC TCTCCAGGGC TTTCTGTCTC CTCTAAACCC TGCTCATCAG 
CTGGGAAATC TCAGGCTTGA AGAGTGTCGA ATTATGTCTT CTGCAAAAAG GCCACTGTGG 
TTGAATTGGG AGAACCCAGA CATCATGTCA GAATTACTCT TTCAGAACAA TGAGATCATC 
TTTAAAAATG GGGATGATTT ACGGCAAGAT ATGCTAACCC TTCAGATTAT TCGCATTATG 
GAAAATATCT GGCAAAATCA AGGTCTTGAT CTTCGAATGT TACCTTATGG ATGTCTGTCA 
ATCGGTGACT GTGTGGGACT TATCGAGGTG GTGAGAAATT CTCACACTAT AATGCAGATT 
CAGTGTAAAG GAGGCCTGAA AGGTGCACTG CAGTTTAACA GCCACACACT CCATCAGTGG 
CTCAAAGACA AGAACAAGGG GGAAATATAT GATGCGGCCA TCGATTTGTT TACACGATCA 
TGTGCTGGAT ATTGTGTTGC CACCTTCATT TTGGGAATTG GAGATCGTCA CAATAGTAAT 
ATCATGGTTA AAGATGATGG ACAACTGTTT CATATAGATT TTGGACACTT TTTGGATCAC 
AAGAAGAAAA AATTTGGTTA TAAACGAGAG CGCGTGCCGT TTGTTTTGAC ACAAGATTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGCACAAAGA CAAGAGAATT TGAGAGGTTT 
CAGGAGATGT GTTACAAGGC TTATCTAGCT ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCAA TGATGCTTGG CTCTGGAATG CCAGAACTGC AATCTTTTGA TGATATTGCA 
TACATTCGAA AGACCCTAGC TTTAGATAAA ACTGAGCAAG AGGCTTTGGA GTATTTCATG 
AAACAAATGA ATGATGCACA CCATGGTGGC TGGACAACAA AAATGGATTG GATCTTCCAC 
ACAATTAAGC AGCATGCTTT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848781.1
CDS	215..3421
Translation	MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLRAICTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN

Target ORF information:

RefSeq Version	XM_024993013.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993013.1

ORF Insert Sequence:

ATGCCTCCAA GACCATCATC AGGTGAACTG TGGGGCATCC ACTTGATGCC CCCAAGAATC 
CTAGTAGAAT GTTTACTACC AAATGGGATG ATAGTGACTT TAGAATGCCT CCGTGAGGCT 
ACGTTAATAA CGATAAAGCA TGAACTATTT AAAGAAGCAA GAAAATACCC TCTCCATCAA 
CTTCTTCAAG ATGAATCTTC TTACATTTTC GTAAGTGTTA CCCAAGAAGC AGAAAGGGAA 
GAATTTTTTG ATGAAACAAG ACGACTTTGT GACCTTCGGC TTTTTCAACC CTTTTTAAAA 
GTAATTGAAC CAGTAGGCAA CCGTGAAGAA AAGATCCTCA ATCGAGAAAT TGGTTTTGCT 
ATCGGCATGC CAGTGTGTGA ATTCGATATG GTTAAAGATC CAGAAGTACA GGACTTCCGA 
AGAAATATTC TCAATGTTTG TAAAGAAGCT GTGGATCTTA GGGATCTTAA TTCACCTCAT 
AGTAGAGCAA TGTATGTTTA TCCTCCAAAT GTAGAATCTT CACCAGAACT GCCAAAGCAC 
ATATATAATA AATTGGATAA AGGGCAAATA ATAGTGGTGA TTTGGGTAAT AGTTTCTCCA 
AATAATGACA AACAGAAGTA TACTCTGAAA ATCAACCATG ACTGTGTGCC AGAACAAGTA 
ATTGCTGAAG CAATCAGGAA AAAAACTCGA AGTATGTTGC TATCATCTGA ACAACTAAAA 
CTCTGTGTTT TAGAATATCA GGGCAAGTAT ATTTTAAAAG TGTGTGGATG TGATGAATAC 
TTCCTAGAAA AATATCCTCT GAGTCAGTAT AAGTATATAA GAAGCTGTAT AATGCTTGGG 
AGGATGCCCA ATTTGATGCT GATGGCTAAA GAAAGCCTCT ATTCTCAACT GCCAATGGAC 
TGTTTTACAA TGCCATCATA TTCCAGACGC ATCTCCACAG CTACGCCATA TATGAATGGA 
GAAACATCTA CAAAATCCCT TTGGGTTATA AATAGTGCAC TCAGAATAAA AATTCTTTGT 
GCAACCTATG TGAATGTAAA TATTCGAGAC ATTGACAAGA TTTATGTTCG AACAGGTATC 
TACCATGGAG GAGAACCCTT ATGTGATAAT GTGAACACTC AAAGAGTACC TTGTTCCAAT 
CCCAGGTGGA ATGAATGGCT GAATTACGAT ATATACATTC CTGATCTTCC TCGTGCTGCT 
CGACTTTGCC TTTCCATTTG TTCTGTTAAA GGCCGAAAGG GTGCTAAAGA GGAACACTGT 
CCATTGGCCT GGGGAAATAT AAACTTGTTT GATTACACAG ATACTCTAGT ATCTGGAAAA 
ATGGCTTTGA ATCTTTGGCC AGTACCTCAT GGACTAGAAG ATTTGCTGAA CCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAAGAAACT CCATGTTTAG AGTTGGAGTT TGACTGGTTC 
AGCAGTGTGG TAAAGTTTCC AGATATGTCA GTGATTGAAG AGCATGCCAA TTGGTCTGTA 
TCCCGTGAAG CAGGATTTAG TTATTCCCAT GCAGGACTGA GTAACAGACT AGCTAGAGAC 
AATGAATTAA GAGAAAATGA TAAAGAACAG CTCCGAGCAA TTTGTACACG AGATCCTCTA 
TCTGAAATCA CTGAGCAAGA GAAAGATTTT CTGTGGAGCC ACAGACACTA TTGTGTAACT 
ATCCCCGAAA TTCTACCCAA ATTGCTTCTG TCTGTTAAAT GGAACTCTAG AGATGAAGTA 
GCTCAGATGT ACTGCTTGGT AAAAGATTGG CCTCCAATCA AGCCTGAACA GGCTATGGAG 
CTTCTGGACT GCAATTACCC AGATCCTATG GTTCGAGGTT TTGCTGTTCG GTGCTTAGAA 
AAATATTTAA CAGATGACAA ACTTTCTCAG TACCTAATTC AGCTAGTACA GGTACTAAAA 
TATGAACAGT ATTTGGATAA CCTGCTTGTG AGATTTTTAC TCAAAAAAGC GTTAACTAAT 
CAAAGGATCG GTCACTTTTT CTTTTGGCAT TTAAAATCTG AGATGCACAA TAAAACAGTT 
AGTCAGAGGT TTGGCCTGCT TTTGGAGTCC TATTGCCGTG CATGTGGGAT GTATCTGAAG 
CACCTTAATA GGCAAGTTGA GGCTATGGAA AAGCTCATTA ACTTGACTGA CATTCTCAAA 
CAAGAGAAGA AGGATGAAAC ACAAAAGGTA CAGATGAAGT TTTTAGTTGA GCAAATGCGG 
CGACCAGATT TCATGGATGC TCTCCAGGGC TTTCTGTCTC CTCTAAACCC TGCTCATCAG 
CTGGGAAATC TCAGGCTTGA AGAGTGTCGA ATTATGTCTT CTGCAAAAAG GCCACTGTGG 
TTGAATTGGG AGAACCCAGA CATCATGTCA GAATTACTCT TTCAGAACAA TGAGATCATC 
TTTAAAAATG GGGATGATTT ACGGCAAGAT ATGCTAACCC TTCAGATTAT TCGCATTATG 
GAAAATATCT GGCAAAATCA AGGTCTTGAT CTTCGAATGT TACCTTATGG ATGTCTGTCA 
ATCGGTGACT GTGTGGGACT TATCGAGGTG GTGAGAAATT CTCACACTAT AATGCAGATT 
CAGTGTAAAG GAGGCCTGAA AGGTGCACTG CAGTTTAACA GCCACACACT CCATCAGTGG 
CTCAAAGACA AGAACAAGGG GGAAATATAT GATGCGGCCA TCGATTTGTT TACACGATCA 
TGTGCTGGAT ATTGTGTTGC CACCTTCATT TTGGGAATTG GAGATCGTCA CAATAGTAAT 
ATCATGGTTA AAGATGATGG ACAACTGTTT CATATAGATT TTGGACACTT TTTGGATCAC 
AAGAAGAAAA AATTTGGTTA TAAACGAGAG CGCGTGCCGT TTGTTTTGAC ACAAGATTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGCACAAAGA CAAGAGAATT TGAGAGGTTT 
CAGGAGATGT GTTACAAGGC TTATCTAGCT ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCAA TGATGCTTGG CTCTGGAATG CCAGAACTGC AATCTTTTGA TGATATTGCA 
TACATTCGAA AGACCCTAGC TTTAGATAAA ACTGAGCAAG AGGCTTTGGA GTATTTCATG 
AAACAAATGA ATGATGCACA CCATGGTGGC TGGACAACAA AAATGGATTG GATCTTCCAC 
ACAATTAAGC AGCATGCTTT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_024993044.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848812.1 Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), transcript variant X2, mRNA.

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CloneID	OBa01285
Clone ID Related Accession (Same CDS sequence)	XM_024993013.1 , NM_174574.1 , XM_024993044.1
Accession Version	XM_024993044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCATCATC AGGTGAACTG TGGGGCATCC ACTTGATGCC CCCAAGAATC 
CTAGTAGAAT GTTTACTACC AAATGGGATG ATAGTGACTT TAGAATGCCT CCGTGAGGCT 
ACGTTAATAA CGATAAAGCA TGAACTATTT AAAGAAGCAA GAAAATACCC TCTCCATCAA 
CTTCTTCAAG ATGAATCTTC TTACATTTTC GTAAGTGTTA CCCAAGAAGC AGAAAGGGAA 
GAATTTTTTG ATGAAACAAG ACGACTTTGT GACCTTCGGC TTTTTCAACC CTTTTTAAAA 
GTAATTGAAC CAGTAGGCAA CCGTGAAGAA AAGATCCTCA ATCGAGAAAT TGGTTTTGCT 
ATCGGCATGC CAGTGTGTGA ATTCGATATG GTTAAAGATC CAGAAGTACA GGACTTCCGA 
AGAAATATTC TCAATGTTTG TAAAGAAGCT GTGGATCTTA GGGATCTTAA TTCACCTCAT 
AGTAGAGCAA TGTATGTTTA TCCTCCAAAT GTAGAATCTT CACCAGAACT GCCAAAGCAC 
ATATATAATA AATTGGATAA AGGGCAAATA ATAGTGGTGA TTTGGGTAAT AGTTTCTCCA 
AATAATGACA AACAGAAGTA TACTCTGAAA ATCAACCATG ACTGTGTGCC AGAACAAGTA 
ATTGCTGAAG CAATCAGGAA AAAAACTCGA AGTATGTTGC TATCATCTGA ACAACTAAAA 
CTCTGTGTTT TAGAATATCA GGGCAAGTAT ATTTTAAAAG TGTGTGGATG TGATGAATAC 
TTCCTAGAAA AATATCCTCT GAGTCAGTAT AAGTATATAA GAAGCTGTAT AATGCTTGGG 
AGGATGCCCA ATTTGATGCT GATGGCTAAA GAAAGCCTCT ATTCTCAACT GCCAATGGAC 
TGTTTTACAA TGCCATCATA TTCCAGACGC ATCTCCACAG CTACGCCATA TATGAATGGA 
GAAACATCTA CAAAATCCCT TTGGGTTATA AATAGTGCAC TCAGAATAAA AATTCTTTGT 
GCAACCTATG TGAATGTAAA TATTCGAGAC ATTGACAAGA TTTATGTTCG AACAGGTATC 
TACCATGGAG GAGAACCCTT ATGTGATAAT GTGAACACTC AAAGAGTACC TTGTTCCAAT 
CCCAGGTGGA ATGAATGGCT GAATTACGAT ATATACATTC CTGATCTTCC TCGTGCTGCT 
CGACTTTGCC TTTCCATTTG TTCTGTTAAA GGCCGAAAGG GTGCTAAAGA GGAACACTGT 
CCATTGGCCT GGGGAAATAT AAACTTGTTT GATTACACAG ATACTCTAGT ATCTGGAAAA 
ATGGCTTTGA ATCTTTGGCC AGTACCTCAT GGACTAGAAG ATTTGCTGAA CCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAAGAAACT CCATGTTTAG AGTTGGAGTT TGACTGGTTC 
AGCAGTGTGG TAAAGTTTCC AGATATGTCA GTGATTGAAG AGCATGCCAA TTGGTCTGTA 
TCCCGTGAAG CAGGATTTAG TTATTCCCAT GCAGGACTGA GTAACAGACT AGCTAGAGAC 
AATGAATTAA GAGAAAATGA TAAAGAACAG CTCCGAGCAA TTTGTACACG AGATCCTCTA 
TCTGAAATCA CTGAGCAAGA GAAAGATTTT CTGTGGAGCC ACAGACACTA TTGTGTAACT 
ATCCCCGAAA TTCTACCCAA ATTGCTTCTG TCTGTTAAAT GGAACTCTAG AGATGAAGTA 
GCTCAGATGT ACTGCTTGGT AAAAGATTGG CCTCCAATCA AGCCTGAACA GGCTATGGAG 
CTTCTGGACT GCAATTACCC AGATCCTATG GTTCGAGGTT TTGCTGTTCG GTGCTTAGAA 
AAATATTTAA CAGATGACAA ACTTTCTCAG TACCTAATTC AGCTAGTACA GGTACTAAAA 
TATGAACAGT ATTTGGATAA CCTGCTTGTG AGATTTTTAC TCAAAAAAGC GTTAACTAAT 
CAAAGGATCG GTCACTTTTT CTTTTGGCAT TTAAAATCTG AGATGCACAA TAAAACAGTT 
AGTCAGAGGT TTGGCCTGCT TTTGGAGTCC TATTGCCGTG CATGTGGGAT GTATCTGAAG 
CACCTTAATA GGCAAGTTGA GGCTATGGAA AAGCTCATTA ACTTGACTGA CATTCTCAAA 
CAAGAGAAGA AGGATGAAAC ACAAAAGGTA CAGATGAAGT TTTTAGTTGA GCAAATGCGG 
CGACCAGATT TCATGGATGC TCTCCAGGGC TTTCTGTCTC CTCTAAACCC TGCTCATCAG 
CTGGGAAATC TCAGGCTTGA AGAGTGTCGA ATTATGTCTT CTGCAAAAAG GCCACTGTGG 
TTGAATTGGG AGAACCCAGA CATCATGTCA GAATTACTCT TTCAGAACAA TGAGATCATC 
TTTAAAAATG GGGATGATTT ACGGCAAGAT ATGCTAACCC TTCAGATTAT TCGCATTATG 
GAAAATATCT GGCAAAATCA AGGTCTTGAT CTTCGAATGT TACCTTATGG ATGTCTGTCA 
ATCGGTGACT GTGTGGGACT TATCGAGGTG GTGAGAAATT CTCACACTAT AATGCAGATT 
CAGTGTAAAG GAGGCCTGAA AGGTGCACTG CAGTTTAACA GCCACACACT CCATCAGTGG 
CTCAAAGACA AGAACAAGGG GGAAATATAT GATGCGGCCA TCGATTTGTT TACACGATCA 
TGTGCTGGAT ATTGTGTTGC CACCTTCATT TTGGGAATTG GAGATCGTCA CAATAGTAAT 
ATCATGGTTA AAGATGATGG ACAACTGTTT CATATAGATT TTGGACACTT TTTGGATCAC 
AAGAAGAAAA AATTTGGTTA TAAACGAGAG CGCGTGCCGT TTGTTTTGAC ACAAGATTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGCACAAAGA CAAGAGAATT TGAGAGGTTT 
CAGGAGATGT GTTACAAGGC TTATCTAGCT ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCAA TGATGCTTGG CTCTGGAATG CCAGAACTGC AATCTTTTGA TGATATTGCA 
TACATTCGAA AGACCCTAGC TTTAGATAAA ACTGAGCAAG AGGCTTTGGA GTATTTCATG 
AAACAAATGA ATGATGCACA CCATGGTGGC TGGACAACAA AAATGGATTG GATCTTCCAC 
ACAATTAAGC AGCATGCTTT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848812.1
CDS	257..3463
Translation	MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLRAICTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN

Target ORF information:

RefSeq Version	XM_024993044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993044.1

ORF Insert Sequence:

ATGCCTCCAA GACCATCATC AGGTGAACTG TGGGGCATCC ACTTGATGCC CCCAAGAATC 
CTAGTAGAAT GTTTACTACC AAATGGGATG ATAGTGACTT TAGAATGCCT CCGTGAGGCT 
ACGTTAATAA CGATAAAGCA TGAACTATTT AAAGAAGCAA GAAAATACCC TCTCCATCAA 
CTTCTTCAAG ATGAATCTTC TTACATTTTC GTAAGTGTTA CCCAAGAAGC AGAAAGGGAA 
GAATTTTTTG ATGAAACAAG ACGACTTTGT GACCTTCGGC TTTTTCAACC CTTTTTAAAA 
GTAATTGAAC CAGTAGGCAA CCGTGAAGAA AAGATCCTCA ATCGAGAAAT TGGTTTTGCT 
ATCGGCATGC CAGTGTGTGA ATTCGATATG GTTAAAGATC CAGAAGTACA GGACTTCCGA 
AGAAATATTC TCAATGTTTG TAAAGAAGCT GTGGATCTTA GGGATCTTAA TTCACCTCAT 
AGTAGAGCAA TGTATGTTTA TCCTCCAAAT GTAGAATCTT CACCAGAACT GCCAAAGCAC 
ATATATAATA AATTGGATAA AGGGCAAATA ATAGTGGTGA TTTGGGTAAT AGTTTCTCCA 
AATAATGACA AACAGAAGTA TACTCTGAAA ATCAACCATG ACTGTGTGCC AGAACAAGTA 
ATTGCTGAAG CAATCAGGAA AAAAACTCGA AGTATGTTGC TATCATCTGA ACAACTAAAA 
CTCTGTGTTT TAGAATATCA GGGCAAGTAT ATTTTAAAAG TGTGTGGATG TGATGAATAC 
TTCCTAGAAA AATATCCTCT GAGTCAGTAT AAGTATATAA GAAGCTGTAT AATGCTTGGG 
AGGATGCCCA ATTTGATGCT GATGGCTAAA GAAAGCCTCT ATTCTCAACT GCCAATGGAC 
TGTTTTACAA TGCCATCATA TTCCAGACGC ATCTCCACAG CTACGCCATA TATGAATGGA 
GAAACATCTA CAAAATCCCT TTGGGTTATA AATAGTGCAC TCAGAATAAA AATTCTTTGT 
GCAACCTATG TGAATGTAAA TATTCGAGAC ATTGACAAGA TTTATGTTCG AACAGGTATC 
TACCATGGAG GAGAACCCTT ATGTGATAAT GTGAACACTC AAAGAGTACC TTGTTCCAAT 
CCCAGGTGGA ATGAATGGCT GAATTACGAT ATATACATTC CTGATCTTCC TCGTGCTGCT 
CGACTTTGCC TTTCCATTTG TTCTGTTAAA GGCCGAAAGG GTGCTAAAGA GGAACACTGT 
CCATTGGCCT GGGGAAATAT AAACTTGTTT GATTACACAG ATACTCTAGT ATCTGGAAAA 
ATGGCTTTGA ATCTTTGGCC AGTACCTCAT GGACTAGAAG ATTTGCTGAA CCCTATTGGT 
GTTACTGGAT CAAATCCAAA TAAAGAAACT CCATGTTTAG AGTTGGAGTT TGACTGGTTC 
AGCAGTGTGG TAAAGTTTCC AGATATGTCA GTGATTGAAG AGCATGCCAA TTGGTCTGTA 
TCCCGTGAAG CAGGATTTAG TTATTCCCAT GCAGGACTGA GTAACAGACT AGCTAGAGAC 
AATGAATTAA GAGAAAATGA TAAAGAACAG CTCCGAGCAA TTTGTACACG AGATCCTCTA 
TCTGAAATCA CTGAGCAAGA GAAAGATTTT CTGTGGAGCC ACAGACACTA TTGTGTAACT 
ATCCCCGAAA TTCTACCCAA ATTGCTTCTG TCTGTTAAAT GGAACTCTAG AGATGAAGTA 
GCTCAGATGT ACTGCTTGGT AAAAGATTGG CCTCCAATCA AGCCTGAACA GGCTATGGAG 
CTTCTGGACT GCAATTACCC AGATCCTATG GTTCGAGGTT TTGCTGTTCG GTGCTTAGAA 
AAATATTTAA CAGATGACAA ACTTTCTCAG TACCTAATTC AGCTAGTACA GGTACTAAAA 
TATGAACAGT ATTTGGATAA CCTGCTTGTG AGATTTTTAC TCAAAAAAGC GTTAACTAAT 
CAAAGGATCG GTCACTTTTT CTTTTGGCAT TTAAAATCTG AGATGCACAA TAAAACAGTT 
AGTCAGAGGT TTGGCCTGCT TTTGGAGTCC TATTGCCGTG CATGTGGGAT GTATCTGAAG 
CACCTTAATA GGCAAGTTGA GGCTATGGAA AAGCTCATTA ACTTGACTGA CATTCTCAAA 
CAAGAGAAGA AGGATGAAAC ACAAAAGGTA CAGATGAAGT TTTTAGTTGA GCAAATGCGG 
CGACCAGATT TCATGGATGC TCTCCAGGGC TTTCTGTCTC CTCTAAACCC TGCTCATCAG 
CTGGGAAATC TCAGGCTTGA AGAGTGTCGA ATTATGTCTT CTGCAAAAAG GCCACTGTGG 
TTGAATTGGG AGAACCCAGA CATCATGTCA GAATTACTCT TTCAGAACAA TGAGATCATC 
TTTAAAAATG GGGATGATTT ACGGCAAGAT ATGCTAACCC TTCAGATTAT TCGCATTATG 
GAAAATATCT GGCAAAATCA AGGTCTTGAT CTTCGAATGT TACCTTATGG ATGTCTGTCA 
ATCGGTGACT GTGTGGGACT TATCGAGGTG GTGAGAAATT CTCACACTAT AATGCAGATT 
CAGTGTAAAG GAGGCCTGAA AGGTGCACTG CAGTTTAACA GCCACACACT CCATCAGTGG 
CTCAAAGACA AGAACAAGGG GGAAATATAT GATGCGGCCA TCGATTTGTT TACACGATCA 
TGTGCTGGAT ATTGTGTTGC CACCTTCATT TTGGGAATTG GAGATCGTCA CAATAGTAAT 
ATCATGGTTA AAGATGATGG ACAACTGTTT CATATAGATT TTGGACACTT TTTGGATCAC 
AAGAAGAAAA AATTTGGTTA TAAACGAGAG CGCGTGCCGT TTGTTTTGAC ACAAGATTTC 
TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGCACAAAGA CAAGAGAATT TGAGAGGTTT 
CAGGAGATGT GTTACAAGGC TTATCTAGCT ATTCGGCAGC ATGCCAATCT CTTCATAAAT 
CTTTTCTCAA TGATGCTTGG CTCTGGAATG CCAGAACTGC AATCTTTTGA TGATATTGCA 
TACATTCGAA AGACCCTAGC TTTAGATAAA ACTGAGCAAG AGGCTTTGGA GTATTTCATG 
AAACAAATGA ATGATGCACA CCATGGTGGC TGGACAACAA AAATGGATTG GATCTTCCAC 
ACAATTAAGC AGCATGCTTT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Class I Phosphoinositide 3-Kinase Exerts a Differential Role on Cell Survival and Cell Trafficking in Retina.
Advances in experimental medicine and biology854363-9(2016)
Azadi S,Brush RS,Anderson RE,Rajala RV

Critical evaluation of the two-state model describing the equilibrium unfolding of the PI3K SH3 domain by time-resolved fluorescence resonance energy transfer.
Biochemistry52(52)9482-96(2013 Dec)
Kishore M,Krishnamoorthy G,Udgaonkar JB

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit.
Science (New York, N.Y.)317(5835)239-42(2007 Jul)
Miled N,Yan Y,Hon WC,Perisic O,Zvelebil M,Inbar Y,Schneidman-Duhovny D,Wolfson HJ,Backer JM,Williams RL

Phosphatidylinositol 3-kinase C2alpha is essential for ATP-dependent priming of neurosecretory granule exocytosis.
Molecular biology of the cell16(10)4841-51(2005 Oct)
Meunier FA,Osborne SL,Hammond GR,Cooke FT,Parker PJ,Domin J,Schiavo G

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PIK3CA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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