HSPA1A cDNA ORF clone, Bos taurus(Cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPA1A
Entrez Gene ID	282254
Full Name	heat shock 70kDa protein 1A
Synonyms	HSP70,HSP70-1,HSP70-2,HSPA1,HSPA1B,HSPA2
Gene Type	protein-coding
Organism	Bos taurus(Cattle)
Genome	Chromosome: 23 Map Location: 23q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_203322.3	NP_976067.3	heat shock 70 kDa protein 1B
NM_203322.2	NP_976067.2	heat shock 70 kDa protein 1B

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSPA1B	NP_005337.2
Bos taurus (cattle)	HSPA1A	NP_976067.2
Mus musculus (house mouse)	Hspa1a	NP_034609.2
Rattus norvegicus (Norway rat)	Hspa1a	NP_114177.2
Drosophila melanogaster (fruit fly)	Hsp70Bbb	NP_788663.1
Arabidopsis thaliana (thale cress)	AT5G02490	NP_195869.1
	HSPA1B	XP_001115060.2
Danio rerio (zebrafish)	hsc70	NP_956908.1
Drosophila melanogaster (fruit fly)	Hsc70-2	NP_524339.1
Saccharomyces cerevisiae (baker's yeast)	SSA4	NP_011029.3
Saccharomyces cerevisiae (baker's yeast)	SSA3	NP_009478.1
Xenopus tropicalis (tropical clawed frog)	hspa1l	NP_001072429.1
Homo sapiens (human)	HSPA1A	NP_005336.3
Drosophila melanogaster (fruit fly)	Hsp70Ba	NP_731716.1
Drosophila melanogaster (fruit fly)	Hsp70Bc	NP_650209.1
Kluyveromyces lactis	KLLA0E20527g	XP_454878.1
Pan troglodytes (chimpanzee)	HSPA1A	XP_003311223.1
Canis lupus familiaris (dog)	LOC102155697	XP_005627221.1
Rattus norvegicus (Norway rat)	Hspa1b	NP_997669.1
Drosophila melanogaster (fruit fly)	Hsp70Ab	NP_524798.2
Drosophila melanogaster (fruit fly)	Hsp68	NP_524474.1
Drosophila melanogaster (fruit fly)	Hsp70Aa	NP_731651.1
Drosophila melanogaster (fruit fly)	Hsp70Bb	NP_524927.2
Eremothecium gossypii	AGOS_AER187W	NP_985044.2
Xenopus tropicalis (tropical clawed frog)	LOC100485441	XP_002937685.1
Xenopus tropicalis (tropical clawed frog)	hsp70	NP_001106370.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential effect of thermal stress on HSP70 expression, nitric oxide production and cell proliferation among native and crossbred dairy cattle.
Bhanuprakash V, Singh U, Sengar G, Sajjanar B, Bhusan B, Raja TV, Alex R, Kumar S, Singh R, Ashish Kumar, Alyethodi RR, Kumar S, Deb R
Journal of thermal biology5918-25(2016 Jul)

Mutations in HSP70-2 gene change the susceptibility to clinical mastitis in Chinese Holstein.
Huang P, Lu C, Li J, Xu J, Liu Z, Wang Q, Wang Z, Huo J, Li H, Teng Y, Cai Y
Gene559(1)62-72(2015 Mar)

Heat Shock Protein 70 Prevents Hyperoxia-Induced Disruption of Lung Endothelial Barrier via Caspase-Dependent and AIF-Dependent Pathways.
Kondrikov D, Fulton D, Dong Z, Su Y
PloS one10(6)e0129343(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Sahiwal cattle the mRNA expression of HSP70 and its protein concentration were higher (P<0.05) during peak summer (44 degrees C) and winter (10 degrees C) as compared to Frieswal cattle. This investigation supports the earlier information on the higher adaptability of indigenous cattle breeds to hot and humid conditions compared to the crossbreds of temperate cattle breeds.
Title: Differential effect of thermal stress on HSP70 expression, nitric oxide production and cell proliferation among native and crossbred dairy cattle.

It is suggested that 9 SNPs increase the susceptibility to mastitis due to their low polymorphisms and can be used as molecular markers to breed the dairy cows resistant to mastitis.
Title: Mutations in HSP70-2 gene change the susceptibility to clinical mastitis in Chinese Holstein.

Inhibition of Hsp70 by using the Hsp70 inhibitor KNK437 or knock down Hsp70 using siRNA exaggerated and overexpression of Hsp70 prevented the second phase disruption of lung endothelial integrity.
Title: Heat Shock Protein 70 Prevents Hyperoxia-Induced Disruption of Lung Endothelial Barrier via Caspase-Dependent and AIF-Dependent Pathways.

Prostaglandin E synthase interacts with inducible heat shock protein 70 after heat stress in bovine primary dermal fibroblast cells.
Title: Prostaglandin E synthase interacts with inducible heat shock protein 70 after heat stress in bovine primary dermal fibroblast cells.

Hsp70-1A is a biomarker of low beef tenderness across the cattle breeds. [HSP70-1a]
Title: Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle.

The following HSPA1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42370	NM_203322.2 Latest version!	Bos taurus heat shock 70kDa protein 1A (HSPA1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa216127	NM_203322.3 Latest version!	Bos taurus heat shock protein family A (Hsp70) member 1A (HSPA1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPA1A ( NM_203322.2 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_976067.2 Bos taurus heat shock 70kDa protein 1A (HSPA1A), mRNA.

Price & Availability↑

CloneID	OBa42370
Clone ID Related Accession (Same CDS sequence)	NM_203322.2
Accession Version	NM_203322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-14
Organism	Bos taurus(Cattle)
Product	heat shock 70 kDa protein 1B
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103083.1. On Aug 28, 2005 this sequence version replaced NM_203322.1. ##Evidence-Data-START## Transcript exon combination :: BC103083.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGAAAA ACATGGCTAT CGGCATCGAC CTGGGCACCA CCTACTCCTG CGTAGGGGTG 
TTCCAGCACG GCAAGGTGGA GATCATCGCC AACGACCAGG GCAACCGCAC CACCCCCAGC 
TACGTGGCCT TCACCGATAC CGAGCGGCTC ATCGGGGATG CGGCCAAGAA CCAGGTGGCG 
CTGAACCCGC AGAACACGGT GTTCGACGCG AAGCGGCTGA TCGGCCGCAA GTTCGGAGAC 
CCGGTGGTGC AGTCGGACAT GAAGCACTGG CCTTTCCGCG TCATCAACGA CGGAGACAAG 
CCTAAGGTGC AGGTGAGCTA CAAAGGGGAG ACCAAGGCGT TCTACCCGGA GGAGATCTCG 
TCGATGGTGC TGACCAAGAT GAAGGAGATC GCCGAGGCGT ACCTGGGCCA CCCGGTGACC 
AACGCGGTGA TCACCGTGCC GGCCTACTTC AACGACTCGC AGCGGCAGGC CACCAAGGAC 
GCGGGGGTGA TCGCGGGGCT GAACGTGCTG AGGATCATCA ACGAGCCCAC GGCCGCCGCC 
ATCACCTACG GCCTGGACAG GACGGGCAAG GGGGAGCGCA ACGTGCTCAT CTTTGATCTG 
GGAGGGGGCA CGTTCGACGT GTCCATCCTG ACGATCGACG ACGGCATCTT CGAGGTGAAG 
GCCACGGCCG GGGACACGCA CCTGGGCGGG GAGGACTTCG ACAACAGGCT GGTGAACCAC 
TTCGTGGAGG AGTTCAAGAG GAAGCACAAG AAGGACATCA GCCAGAACAA GCGGGCCGTG 
AGGCGGCTGC GCACCGCATG CGAGCGGGCC AAGAGAACCT TGTCGTCCAG CACCCAGGCC 
AGCCTGGAGA TCGACTCCCT GTTCGAGGGC ATCGACTTCT ACACGTCCAT CACCAGGGCG 
CGGTTCGAGG AGCTGTGCTC CGACCTGTTC CGGAGCACCC TGGAGCCCGT GGAGAAGGCG 
CTACGCGACG CCAAGCTGGA CAAGGCGCAG ATCCACGACC TGGTCCTGGT GGGGGGCTCC 
ACCCGCATCC CCAAGGTGCA GAAGCTGCTG CAGGACTTCT TCAACGGGCG CGACCTCAAC 
AAGAGCATCA ACCCCGACGA GGCGGTGGCG TACGGGGCGG CGGTGCAGGC GGCCATCCTG 
ATGGGGGACA AGTCGGAGAA CGTGCAGGAC CTGCTGTTGC TGGACGTGGC TCCCCTGTCG 
CTGGGACTGG AGACGGCCGG AGGCGTGATG ACCGCCCTGA TCAAGCGCAA CTCCACCATC 
CCCACGAAGC AGACGCAGAT CTTCACCACC TACTCGGACA ACCAGCCGGG CGTGCTGATC 
CAGGTGTACG AGGGCGAGAG GGCCATGACG CGGGACAACA ACCTGCTGGG GCGCTTCGAG 
CTGAGCGGCA TCCCGCCGGC CCCGCGGGGG GTGCCCCAGA TCGAGGTGAC CTTCGACATC 
GACGCCAATG GCATCCTGAA CGTCACGGCC ACGGACAAGA GCACGGGCAA GGCCAACAAG 
ATCACCATCA CCAACGACAA GGGCCGGCTG AGCAAGGAGG AGATCGAGCG CATGGTGCAG 
GAGGCGGAAA AGTACAAGGC GGAGGACGAG GTCCAGCGCG AGAGGGTGTC TGCCAAGAAC 
GCGCTGGAGT CGTACGCCTT CAACATGAAG AGCGCCGTGG AGGATGAGGG GCTGAAGGGC 
AAGATCAGCG AGGCGGACAA GAAGAAGGTG CTGGACAAGT GCCAGGAGGT GATTTCCTGG 
CTGGACGCCA ACACCTTGGC GGAGAAGGAC GAGTTTGAGC ACAAGAGGAA GGAGCTGGAG 
CAGGTGTGTA ACCCCATCAT CAGCAGACTG TACCAGGGGG CGGGCGGCCC CGGGGCTGGC 
GGCTTTGGGG CTCAGGGCCC TAAAGGGGGC TCTGGGTCTG GCCCCACCAT TGAGGAGGTG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976067.2
CDS	173..2098
Translation	MAKNMAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFRVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGHPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAITYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISRLYQGAGGPGAGGFGAQGPKGGSGSGPTIEEVD

Target ORF information:

RefSeq Version	NM_203322.2
Organism	Bos taurus(Cattle)
Definition	Bos taurus heat shock 70kDa protein 1A (HSPA1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203322.2

ORF Insert Sequence:

ATGGCGAAAA ACATGGCTAT CGGCATCGAC CTGGGCACCA CCTACTCCTG CGTAGGGGTG 
TTCCAGCACG GCAAGGTGGA GATCATCGCC AACGACCAGG GCAACCGCAC CACCCCCAGC 
TACGTGGCCT TCACCGATAC CGAGCGGCTC ATCGGGGATG CGGCCAAGAA CCAGGTGGCG 
CTGAACCCGC AGAACACGGT GTTCGACGCG AAGCGGCTGA TCGGCCGCAA GTTCGGAGAC 
CCGGTGGTGC AGTCGGACAT GAAGCACTGG CCTTTCCGCG TCATCAACGA CGGAGACAAG 
CCTAAGGTGC AGGTGAGCTA CAAAGGGGAG ACCAAGGCGT TCTACCCGGA GGAGATCTCG 
TCGATGGTGC TGACCAAGAT GAAGGAGATC GCCGAGGCGT ACCTGGGCCA CCCGGTGACC 
AACGCGGTGA TCACCGTGCC GGCCTACTTC AACGACTCGC AGCGGCAGGC CACCAAGGAC 
GCGGGGGTGA TCGCGGGGCT GAACGTGCTG AGGATCATCA ACGAGCCCAC GGCCGCCGCC 
ATCACCTACG GCCTGGACAG GACGGGCAAG GGGGAGCGCA ACGTGCTCAT CTTTGATCTG 
GGAGGGGGCA CGTTCGACGT GTCCATCCTG ACGATCGACG ACGGCATCTT CGAGGTGAAG 
GCCACGGCCG GGGACACGCA CCTGGGCGGG GAGGACTTCG ACAACAGGCT GGTGAACCAC 
TTCGTGGAGG AGTTCAAGAG GAAGCACAAG AAGGACATCA GCCAGAACAA GCGGGCCGTG 
AGGCGGCTGC GCACCGCATG CGAGCGGGCC AAGAGAACCT TGTCGTCCAG CACCCAGGCC 
AGCCTGGAGA TCGACTCCCT GTTCGAGGGC ATCGACTTCT ACACGTCCAT CACCAGGGCG 
CGGTTCGAGG AGCTGTGCTC CGACCTGTTC CGGAGCACCC TGGAGCCCGT GGAGAAGGCG 
CTACGCGACG CCAAGCTGGA CAAGGCGCAG ATCCACGACC TGGTCCTGGT GGGGGGCTCC 
ACCCGCATCC CCAAGGTGCA GAAGCTGCTG CAGGACTTCT TCAACGGGCG CGACCTCAAC 
AAGAGCATCA ACCCCGACGA GGCGGTGGCG TACGGGGCGG CGGTGCAGGC GGCCATCCTG 
ATGGGGGACA AGTCGGAGAA CGTGCAGGAC CTGCTGTTGC TGGACGTGGC TCCCCTGTCG 
CTGGGACTGG AGACGGCCGG AGGCGTGATG ACCGCCCTGA TCAAGCGCAA CTCCACCATC 
CCCACGAAGC AGACGCAGAT CTTCACCACC TACTCGGACA ACCAGCCGGG CGTGCTGATC 
CAGGTGTACG AGGGCGAGAG GGCCATGACG CGGGACAACA ACCTGCTGGG GCGCTTCGAG 
CTGAGCGGCA TCCCGCCGGC CCCGCGGGGG GTGCCCCAGA TCGAGGTGAC CTTCGACATC 
GACGCCAATG GCATCCTGAA CGTCACGGCC ACGGACAAGA GCACGGGCAA GGCCAACAAG 
ATCACCATCA CCAACGACAA GGGCCGGCTG AGCAAGGAGG AGATCGAGCG CATGGTGCAG 
GAGGCGGAAA AGTACAAGGC GGAGGACGAG GTCCAGCGCG AGAGGGTGTC TGCCAAGAAC 
GCGCTGGAGT CGTACGCCTT CAACATGAAG AGCGCCGTGG AGGATGAGGG GCTGAAGGGC 
AAGATCAGCG AGGCGGACAA GAAGAAGGTG CTGGACAAGT GCCAGGAGGT GATTTCCTGG 
CTGGACGCCA ACACCTTGGC GGAGAAGGAC GAGTTTGAGC ACAAGAGGAA GGAGCTGGAG 
CAGGTGTGTA ACCCCATCAT CAGCAGACTG TACCAGGGGG CGGGCGGCCC CGGGGCTGGC 
GGCTTTGGGG CTCAGGGCCC TAAAGGGGGC TCTGGGTCTG GCCCCACCAT TGAGGAGGTG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPA1A ( NM_203322.3 ) cDNA ORF clone, Bos taurus(Cattle) -> NP_976067.3 Bos taurus heat shock protein family A (Hsp70) member 1A (HSPA1A), mRNA.

Price & Availability↑

CloneID	OBa216127
Clone ID Related Accession (Same CDS sequence)	NM_203322.3
Accession Version	NM_203322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Bos taurus(cattle)
Product	heat shock 70 kDa protein 1B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000023.1. On or before Jun 2, 2017 this sequence version replaced NM_174550.1, NM_203322.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCGAAAA ACATGGCTAT CGGCATCGAC CTGGGCACCA CCTACTCCTG CGTAGGGGTG 
TTCCAGCACG GCAAGGTGGA GATCATCGCC AACGACCAGG GCAACCGCAC CACCCCCAGC 
TACGTGGCCT TCACCGATAC CGAGCGGCTC ATCGGGGATG CGGCCAAGAA CCAGGTGGCG 
CTGAACCCGC AGAACACGGT GTTCGACGCG AAGCGGCTGA TCGGCCGCAA GTTCGGAGAC 
CCGGTGGTGC AGTCGGACAT GAAGCACTGG CCTTTCCGCG TCATCAACGA CGGAGACAAG 
CCTAAGGTGC AGGTGAGCTA CAAAGGGGAG ACCAAGGCGT TCTACCCGGA GGAGATCTCG 
TCGATGGTGC TGACCAAGAT GAAGGAGATC GCCGAGGCGT ACCTGGGCCA CCCGGTGACC 
AACGCGGTGA TCACCGTGCC GGCCTACTTC AACGACTCGC AGCGGCAGGC CACCAAGGAC 
GCGGGGGTGA TCGCGGGGCT GAACGTGCTG AGGATCATCA ACGAGCCCAC GGCCGCCGCC 
ATCGCCTACG GCCTGGACAG GACGGGCAAG GGGGAGCGCA ACGTGCTCAT CTTTGATCTG 
GGAGGGGGCA CGTTCGACGT GTCCATCCTG ACGATCGACG ACGGCATCTT CGAGGTGAAG 
GCCACGGCCG GGGACACGCA CCTGGGCGGG GAGGACTTCG ACAACAGGCT GGTGAACCAC 
TTCGTGGAGG AGTTCAAGAG GAAGCACAAG AAGGACATCA GCCAGAACAA GCGGGCCGTG 
AGGCGGCTGC GCACCGCATG CGAGCGGGCC AAGAGAACCT TGTCGTCCAG CACCCAGGCC 
AGCCTGGAGA TCGACTCCCT GTTCGAGGGC ATCGACTTCT ACACGTCCAT CACCAGGGCG 
CGGTTCGAGG AGCTGTGCTC CGACCTGTTC CGGAGCACCC TGGAGCCCGT GGAGAAGGCG 
CTACGCGACG CCAAGCTGGA CAAGGCGCAG ATCCACGACC TGGTCCTGGT GGGGGGCTCC 
ACCCGCATCC CCAAGGTGCA GAAGCTGCTG CAGGACTTCT TCAACGGGCG CGACCTCAAC 
AAGAGCATCA ACCCCGACGA GGCGGTGGCG TACGGGGCGG CGGTGCAGGC GGCCATCCTG 
ATGGGGGACA AGTCGGAGAA CGTGCAGGAC CTGCTGTTGC TGGACGTGGC TCCCCTGTCG 
CTGGGACTGG AGACGGCCGG AGGCGTGATG ACCGCCCTGA TCAAGCGCAA CTCCACCATC 
CCCACGAAGC AGACGCAGAT CTTCACCACC TACTCGGACA ACCAGCCGGG CGTGCTGATC 
CAGGTGTACG AGGGCGAGAG GGCCATGACG CGGGACAACA ACCTGCTGGG GCGCTTCGAG 
CTGAGCGGCA TCCCGCCGGC CCCGCGGGGG GTGCCCCAGA TCGAGGTGAC CTTCGACATC 
GACGCCAATG GCATCCTGAA CGTCACGGCC ACGGACAAGA GCACGGGCAA GGCCAACAAG 
ATCACCATCA CCAACGACAA GGGCCGGCTG AGCAAGGAGG AGATCGAGCG CATGGTGCAG 
GAGGCGGAAA AGTACAAGGC GGAGGACGAG GTCCAGCGCG AGAGGGTGTC TGCCAAGAAC 
GCGCTGGAGT CGTACGCCTT CAACATGAAG AGCGCCGTGG AGGATGAGGG GCTGAAGGGC 
AAGATCAGCG AGGCGGACAA GAAGAAGGTG CTGGACAAGT GCCAGGAGGT GATTTCCTGG 
CTGGACGCCA ACACCTTGGC GGAGAAGGAC GAGTTTGAGC ACAAGAGGAA GGAGCTGGAG 
CAGGTGTGTA ACCCCATCAT CAGCAGACTG TACCAGGGGG CGGGCGGCCC CGGGGCTGGC 
GGCTTTGGGG CTCAGGGCCC TAAAGGGGGC TCTGGGTCTG GCCCCACCAT TGAGGAGGTG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976067.3
CDS	212..2137
Translation	MAKNMAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFRVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGHPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISRLYQGAGGPGAGGFGAQGPKGGSGSGPTIEEVD

Target ORF information:

RefSeq Version	NM_203322.3
Organism	Bos taurus(cattle)
Definition	Bos taurus heat shock protein family A (Hsp70) member 1A (HSPA1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203322.3

ORF Insert Sequence:

ATGGCGAAAA ACATGGCTAT CGGCATCGAC CTGGGCACCA CCTACTCCTG CGTAGGGGTG 
TTCCAGCACG GCAAGGTGGA GATCATCGCC AACGACCAGG GCAACCGCAC CACCCCCAGC 
TACGTGGCCT TCACCGATAC CGAGCGGCTC ATCGGGGATG CGGCCAAGAA CCAGGTGGCG 
CTGAACCCGC AGAACACGGT GTTCGACGCG AAGCGGCTGA TCGGCCGCAA GTTCGGAGAC 
CCGGTGGTGC AGTCGGACAT GAAGCACTGG CCTTTCCGCG TCATCAACGA CGGAGACAAG 
CCTAAGGTGC AGGTGAGCTA CAAAGGGGAG ACCAAGGCGT TCTACCCGGA GGAGATCTCG 
TCGATGGTGC TGACCAAGAT GAAGGAGATC GCCGAGGCGT ACCTGGGCCA CCCGGTGACC 
AACGCGGTGA TCACCGTGCC GGCCTACTTC AACGACTCGC AGCGGCAGGC CACCAAGGAC 
GCGGGGGTGA TCGCGGGGCT GAACGTGCTG AGGATCATCA ACGAGCCCAC GGCCGCCGCC 
ATCGCCTACG GCCTGGACAG GACGGGCAAG GGGGAGCGCA ACGTGCTCAT CTTTGATCTG 
GGAGGGGGCA CGTTCGACGT GTCCATCCTG ACGATCGACG ACGGCATCTT CGAGGTGAAG 
GCCACGGCCG GGGACACGCA CCTGGGCGGG GAGGACTTCG ACAACAGGCT GGTGAACCAC 
TTCGTGGAGG AGTTCAAGAG GAAGCACAAG AAGGACATCA GCCAGAACAA GCGGGCCGTG 
AGGCGGCTGC GCACCGCATG CGAGCGGGCC AAGAGAACCT TGTCGTCCAG CACCCAGGCC 
AGCCTGGAGA TCGACTCCCT GTTCGAGGGC ATCGACTTCT ACACGTCCAT CACCAGGGCG 
CGGTTCGAGG AGCTGTGCTC CGACCTGTTC CGGAGCACCC TGGAGCCCGT GGAGAAGGCG 
CTACGCGACG CCAAGCTGGA CAAGGCGCAG ATCCACGACC TGGTCCTGGT GGGGGGCTCC 
ACCCGCATCC CCAAGGTGCA GAAGCTGCTG CAGGACTTCT TCAACGGGCG CGACCTCAAC 
AAGAGCATCA ACCCCGACGA GGCGGTGGCG TACGGGGCGG CGGTGCAGGC GGCCATCCTG 
ATGGGGGACA AGTCGGAGAA CGTGCAGGAC CTGCTGTTGC TGGACGTGGC TCCCCTGTCG 
CTGGGACTGG AGACGGCCGG AGGCGTGATG ACCGCCCTGA TCAAGCGCAA CTCCACCATC 
CCCACGAAGC AGACGCAGAT CTTCACCACC TACTCGGACA ACCAGCCGGG CGTGCTGATC 
CAGGTGTACG AGGGCGAGAG GGCCATGACG CGGGACAACA ACCTGCTGGG GCGCTTCGAG 
CTGAGCGGCA TCCCGCCGGC CCCGCGGGGG GTGCCCCAGA TCGAGGTGAC CTTCGACATC 
GACGCCAATG GCATCCTGAA CGTCACGGCC ACGGACAAGA GCACGGGCAA GGCCAACAAG 
ATCACCATCA CCAACGACAA GGGCCGGCTG AGCAAGGAGG AGATCGAGCG CATGGTGCAG 
GAGGCGGAAA AGTACAAGGC GGAGGACGAG GTCCAGCGCG AGAGGGTGTC TGCCAAGAAC 
GCGCTGGAGT CGTACGCCTT CAACATGAAG AGCGCCGTGG AGGATGAGGG GCTGAAGGGC 
AAGATCAGCG AGGCGGACAA GAAGAAGGTG CTGGACAAGT GCCAGGAGGT GATTTCCTGG 
CTGGACGCCA ACACCTTGGC GGAGAAGGAC GAGTTTGAGC ACAAGAGGAA GGAGCTGGAG 
CAGGTGTGTA ACCCCATCAT CAGCAGACTG TACCAGGGGG CGGGCGGCCC CGGGGCTGGC 
GGCTTTGGGG CTCAGGGCCC TAAAGGGGGC TCTGGGTCTG GCCCCACCAT TGAGGAGGTG 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential effect of thermal stress on HSP70 expression, nitric oxide production and cell proliferation among native and crossbred dairy cattle.
Journal of thermal biology5918-25(2016 Jul)
Bhanuprakash V,Singh U,Sengar G,Sajjanar B,Bhusan B,Raja TV,Alex R,Kumar S,Singh R,Ashish Kumar,Alyethodi RR,Kumar S,Deb R

Mutations in HSP70-2 gene change the susceptibility to clinical mastitis in Chinese Holstein.
Gene559(1)62-72(2015 Mar)
Huang P,Lu C,Li J,Xu J,Liu Z,Wang Q,Wang Z,Huo J,Li H,Teng Y,Cai Y

Heat Shock Protein 70 Prevents Hyperoxia-Induced Disruption of Lung Endothelial Barrier via Caspase-Dependent and AIF-Dependent Pathways.
PloS one10(6)e0129343(2015)
Kondrikov D,Fulton D,Dong Z,Su Y

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HSPA1A cDNA ORF clone, Bos taurus(Cattle)

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