GUCA1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCA1A
Entrez Gene ID	282243
Full Name	guanylate cyclase activator 1A
General protein information	Preferred Names guanylate cyclase activator 1A Names guanylyl cyclase-activating protein 1 GCAP 1 guanylate cyclase activator 1A (retina)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174546.2	NP_776971.1	guanylyl cyclase-activating protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GUCA1A	XP_001174348.2
Bos taurus (cattle)	GUCA1A	NP_776971.1
Rattus norvegicus (Norway rat)	Guca1a	NP_001100357.1
Gallus gallus (chicken)	GUCA1A	NP_989651.1
Canis lupus familiaris (dog)	GUCA1A	XP_851487.1
Mus musculus (house mouse)	Guca1a	NP_032215.2
Danio rerio (zebrafish)	guca1e	NP_956950.1
Danio rerio (zebrafish)	si:ch211-103a14.5	XP_002666176.1
Homo sapiens (human)	GUCA1A	NP_000400.2
	GUCA1C	XP_001086886.1
Xenopus tropicalis (tropical clawed frog)	LOC100124863	NP_001096291.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of Guanylyl Cyclase Activator Protein 1 (GCAP1) Mutant V77E in a Ca2+-free/Mg2+-bound Activator State.
Lim S, Peshenko IV, Olshevskaya EV, Dizhoor AM, Ames JB
The Journal of biological chemistry291(9)4429-41(2016 Feb)

The dimerization domain in outer segment guanylate cyclase is a Ca??-sensitive control switch module.
Z?gel P, Dell'Orco D, Koch KW
Biochemistry52(30)5065-74(2013 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Ca2+ and Mg2+ binding properties of GCAP-1. Evidence that Mg2+-bound form is the physiological activator of photoreceptor guanylyl cyclase.
Peshenko IV, Dizhoor AM
The Journal of biological chemistry281(33)23830-41(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that GCAP1 (Mg2+ vs. Ca2+) exhibits conformational changes in Ca2+ switch helix that are important in activation of RetGC1 (photoreceptor guanylate cyclase); myristoylation of GCAP1 is important as well in attaining activator conformation.
Title: Structure of Guanylyl Cyclase Activator Protein 1 (GCAP1) Mutant V77E in a Ca2+-free/Mg2+-bound Activator State.

Data suggest that dimerization domain of GUCY2D operates as a calcium-sensitive regulatory module; GUCY2D requires correct conformation of monomer-monomer interface for interaction with guanylate cyclase activating proteins (GCAP1; GCAP2).
Title: The dimerization domain in outer segment guanylate cyclase is a Ca??-sensitive control switch module.

Presence of bound magnesium (Mg2+) in guanylate cyclase activator protein GCAP-1 is essential for its ability to stimulate retinal guanylyl cyclase.
Title: Ca2+ and Mg2+ binding properties of GCAP-1. Evidence that Mg2+-bound form is the physiological activator of photoreceptor guanylyl cyclase.

The following GUCA1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCA1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa00789	NM_174546.2 Latest version!	Bos taurus guanylate cyclase activator 1A (GUCA1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCA1A ( NM_174546.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776971.1 Bos taurus guanylate cyclase activator 1A (GUCA1A), mRNA.

Price & Availability↑

CloneID	OBa00789
Clone ID Related Accession (Same CDS sequence)	NM_174546.2
Accession Version	NM_174546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	guanylyl cyclase-activating protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X95352.1. On Aug 30, 2012 this sequence version replaced NM_174546.1. ##Evidence-Data-START## CDS exon combination :: X95352.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145425, SAMN03145455 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAACA TTATGGACGG TAAGTCGGTG GAGGAGCTGA GCAGCACCGA GTGCCACCAG 
TGGTACAAGA AGTTCATGAC AGAGTGCCCC TCCGGCCAGC TCACCCTCTA CGAGTTCCGC 
CAGTTCTTCG GCCTCAAGAA CCTGAGCCCG TGGGCCAGCC AGTACGTGGA GCAGATGTTT 
GAGACCTTTG ACTTCAACAA AGACGGCTAC ATTGATTTCA TGGAGTACGT GGCGGCTCTG 
AGCCTGGTCC TCAAGGGGAA GGTGGAACAG AAGCTGCGTT GGTACTTCAA GCTCTACGAC 
GTGGACGGCA ACGGATGCAT CGACCGCGAC GAGCTGCTCA CCATCATCCG GGCCATCCGA 
GCCATTAACC CCTGCAGCGA CTCGACCATG ACCGCCGAGG AGTTCACCGA TACAGTGTTC 
TCCAAGATTG ACGTCAATGG GGATGGGGAA CTCTCCCTAG AGGAGTTCAT GGAGGGCGTC 
CAGAAGGACC AGATGCTCTT GGACACGCTG ACCCGAAGCT TGGACCTTAC CCGCATTGTG 
CGCAGGCTCC AGAATGGAGA GCAGGATGAG GAGGGGGCTA GCGGCAGGGA AACGGAGGCC 
GCGGAGGCCG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776971.1
CDS	211..828
Translation	MGNIMDGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSPWASQYVEQMFETFDFNKDGYIDFMEYVAALSLVLKGKVEQKLRWYFKLYDVDGNGCIDRDELLTIIRAIRAINPCSDSTMTAEEFTDTVFSKIDVNGDGELSLEEFMEGVQKDQMLLDTLTRSLDLTRIVRRLQNGEQDEEGASGRETEAAEADG

Target ORF information:

RefSeq Version	NM_174546.2
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase activator 1A (GUCA1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174546.2

ORF Insert Sequence:

ATGGGGAACA TTATGGACGG TAAGTCGGTG GAGGAGCTGA GCAGCACCGA GTGCCACCAG 
TGGTACAAGA AGTTCATGAC AGAGTGCCCC TCCGGCCAGC TCACCCTCTA CGAGTTCCGC 
CAGTTCTTCG GCCTCAAGAA CCTGAGCCCG TGGGCCAGCC AGTACGTGGA GCAGATGTTT 
GAGACCTTTG ACTTCAACAA AGACGGCTAC ATTGATTTCA TGGAGTACGT GGCGGCTCTG 
AGCCTGGTCC TCAAGGGGAA GGTGGAACAG AAGCTGCGTT GGTACTTCAA GCTCTACGAC 
GTGGACGGCA ACGGATGCAT CGACCGCGAC GAGCTGCTCA CCATCATCCG GGCCATCCGA 
GCCATTAACC CCTGCAGCGA CTCGACCATG ACCGCCGAGG AGTTCACCGA TACAGTGTTC 
TCCAAGATTG ACGTCAATGG GGATGGGGAA CTCTCCCTAG AGGAGTTCAT GGAGGGCGTC 
CAGAAGGACC AGATGCTCTT GGACACGCTG ACCCGAAGCT TGGACCTTAC CCGCATTGTG 
CGCAGGCTCC AGAATGGAGA GCAGGATGAG GAGGGGGCTA GCGGCAGGGA AACGGAGGCC 
GCGGAGGCCG ACGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of Guanylyl Cyclase Activator Protein 1 (GCAP1) Mutant V77E in a Ca2+-free/Mg2+-bound Activator State.
The Journal of biological chemistry291(9)4429-41(2016 Feb)
Lim S,Peshenko IV,Olshevskaya EV,Dizhoor AM,Ames JB

The dimerization domain in outer segment guanylate cyclase is a Ca??-sensitive control switch module.
Biochemistry52(30)5065-74(2013 Jul)
Z?gel P,Dell'Orco D,Koch KW

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Ca2+ and Mg2+ binding properties of GCAP-1. Evidence that Mg2+-bound form is the physiological activator of photoreceptor guanylyl cyclase.
The Journal of biological chemistry281(33)23830-41(2006 Aug)
Peshenko IV,Dizhoor AM

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GUCA1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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