gRNAs
The following COL4A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa239255 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001166511.3 | |
| Accession Version | NM_001166511.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-22 | |
| Organism | Bos taurus(cattle) | |
| Product | collagen alpha-1(IV) chain precursor | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000012.1. On May 29, 2018 this sequence version replaced NM_001166511.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571261.5929.1, SRR5765710.6779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145432 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGGGCCCC GGCTCGGCGT CTGGCTGCTG CTGCTGCTCG CCGCGCTCCT GCTCCACGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001159983.2 |
| CDS | 17..5026 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001166511.3 |
1 | ATGGGGCCCC GGCTCGGCGT CTGGCTGCTG CTGCTGCTCG CCGCGCTCCT GCTCCACGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa216124 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001166511.2 | |
| Accession Version | NM_001166511.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-27 | |
| Organism | Bos taurus(cattle) | |
| Product | collagen alpha-1(IV) chain precursor | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02034909.1, DAAA02034908.1 and DAAA02034907.1. On Jun 30, 2017 this sequence version replaced NM_001166511.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGGGCCCC GGCTCGGCGT CTGGCTGCTG CTGCTGCTCG CCGCGCTCCT GCTCCACGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001159983.2 |
| CDS | 17..5026 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001166511.2 |
1 | ATGGGGCCCC GGCTCGGCGT CTGGCTGCTG CTGCTGCTCG CCGCGCTCCT GCTCCACGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa44513 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001166511.1 | |
| Accession Version | NM_001166511.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-01-03 | |
| Organism | Bos taurus(Cattle) | |
| Product | collagen alpha-1(IV) chain precursor | |
| Comment | PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03033811.1, AAFC03061689.1 and AAFC03033807.1. On Oct 27, 2009 this sequence version replaced XM_580317.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093727, SAMN01093732 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGGGCCCC GGCTAGGCGT CTGGCTGATG CTGCTGCTCG CCGCGCTCCT GCTCCACGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001159983.1 |
| CDS | 17..5026 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001166511.1 |
1 | ATGGGGCCCC GGCTAGGCGT CTGGCTGATG CTGCTGCTCG CCGCGCTCCT GCTCCACGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Laminar high shear stress up-regulates type IV collagen synthesis and down-regulates MMP-2 secretion in endothelium. A quantitative analysis. |
A whole-genome assembly of the domestic cow, Bos taurus. |
The alpha1.alpha2 network of collagen IV. Reinforced stabilization of the noncollagenous domain-1 by noncovalent forces and the absence of Met-Lys cross-links. |
Crystal structure of NC1 domains. Structural basis for type IV collagen assembly in basement membranes. |
