gRNAs
The following ADAM10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAM10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa41466 | |
| Clone ID Related Accession (Same CDS sequence) | NM_174496.2 | |
| Accession Version | NM_174496.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-15 | |
| Organism | Bos taurus(cattle) | |
| Product | disintegrin and metalloproteinase domain-containing protein 10 preproprotein | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z21961.1. On or before Mar 29, 2005 this sequence version replaced XM_607174.1, NM_174496.1. ##Evidence-Data-START## Transcript exon combination :: Z21961.1, BC153863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145422 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGTGTTGC TGAGAGTGTT AATTCTGCTC CTCTCCTGGG TTGCGGGGCT GGGAGGTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_776921.1 |
| CDS | 17..2263 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_174496.2 |
1 | ATGGTGTTGC TGAGAGTGTT AATTCTGCTC CTCTCCTGGG TTGCGGGGCT GGGAGGTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa258711 | |
| Clone ID Related Accession (Same CDS sequence) | NM_174496.3 | |
| Accession Version | NM_174496.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-25 | |
| Organism | Bos taurus(cattle) | |
| Product | disintegrin and metalloproteinase domain-containing protein 10 preproprotein | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000010.1. On May 15, 2019 this sequence version replaced NM_174496.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571262.34802.1, BC153863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145422 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGTGTTGC TGAGAGTGTT AATTCTGCTC CTCTCCTGGG TTGCGGGGCT GGGAGGTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_776921.1 |
| CDS | 226..2472 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_174496.3 |
1 | ATGGTGTTGC TGAGAGTGTT AATTCTGCTC CTCTCCTGGG TTGCGGGGCT GGGAGGTCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa219411 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024997276.1 , XM_024997275.1 | |
| Accession Version | XM_024997276.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | disintegrin and metalloproteinase domain-containing protein 10 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGAGGG ATACTTCCCT TTTCAGTGAG GAGTTTAGGG TGGAAACATC AAATGCAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024853044.1 |
| CDS | 339..2363 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024997276.1 |
1 | ATGAAGAGGG ATACTTCCCT TTTCAGTGAG GAGTTTAGGG TGGAAACATC AAATGCAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa219411 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024997276.1 , XM_024997275.1 | |
| Accession Version | XM_024997275.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | disintegrin and metalloproteinase domain-containing protein 10 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAGAGGG ATACTTCCCT TTTCAGTGAG GAGTTTAGGG TGGAAACATC AAATGCAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024853043.1 |
| CDS | 646..2670 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024997275.1 |
1 | ATGAAGAGGG ATACTTCCCT TTTCAGTGAG GAGTTTAGGG TGGAAACATC AAATGCAGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The TspanC8 subgroup of tetraspanins interacts with A disintegrin and metalloprotease 10 (ADAM10) and regulates its maturation and cell surface expression. |
A disintegrin and metalloprotease 10 activity sheds the ectodomain of the amyloid precursor-like protein 2 and regulates protein expression in proximal tubule cells. |
A whole-genome assembly of the domestic cow, Bos taurus. |
Functional role of N-glycosylation from ADAM10 in processing, localization and activity of the enzyme. |
A basolateral sorting signal directs ADAM10 to adherens junctions and is required for its function in cell migration. |
