VCAM1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCAM1
Entrez Gene ID	282118
Full Name	vascular cell adhesion molecule 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174484.1	NP_776909.1	vascular cell adhesion molecule 1 precursor
XM_005204079.3	XP_005204136.1	vascular cell adhesion molecule 1 isoform X1
XM_005204079.4	XP_005204136.1	vascular cell adhesion molecule 1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	vcam1	NP_001077304.1
Homo sapiens (human)	VCAM1	NP_001069.1
	VCAM1	XP_001107924.1
Bos taurus (cattle)	LOC534578	NP_001094628.1
Mus musculus (house mouse)	Vcam1	NP_035823.3
Rattus norvegicus (Norway rat)	Vcam1	NP_037021.1
Canis lupus familiaris (dog)	VCAM1	NP_001003298.1
Bos taurus (cattle)	VCAM1	NP_776909.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase A-alpha directly phosphorylates FoxO1 in vascular endothelial cells to regulate expression of vascular cellular adhesion molecule-1 mRNA.
Lee JW, Chen H, Pullikotil P, Quon MJ
The Journal of biological chemistry286(8)6423-32(2011 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

CAMP-response element-binding protein mediates tumor necrosis factor-alpha-induced vascular cell adhesion molecule-1 expression in endothelial cells.
Ono H, Ichiki T, Ohtsubo H, Fukuyama K, Imayama I, Iino N, Masuda S, Hashiguchi Y, Takeshita A, Sunagawa K
Hypertension research : official journal of the Japanese Society of Hypertension29(1)39-47(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Protein kinase A-alpha directly phosphorylates FoxO1 in vascular endothelial cells to regulate expression of vascular cellular adhesion molecule-1 mRNA.
Title: Protein kinase A-alpha directly phosphorylates FoxO1 in vascular endothelial cells to regulate expression of vascular cellular adhesion molecule-1 mRNA.

the p38-MAPK/CREB pathway plays a critical role in TNFalpha-induced VCAM-1 expression
Title: CAMP-response element-binding protein mediates tumor necrosis factor-alpha-induced vascular cell adhesion molecule-1 expression in endothelial cells.

The following VCAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa17566	XM_005204079.4 Latest version!	Bos taurus vascular cell adhesion molecule 1 (VCAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa39439	NM_174484.1 Latest version!	Bos taurus vascular cell adhesion molecule 1 (VCAM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa17566	XM_005204079.3	Bos taurus vascular cell adhesion molecule 1 (VCAM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCAM1 ( XM_005204079.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204136.1 Bos taurus vascular cell adhesion molecule 1 (VCAM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa17566
Clone ID Related Accession (Same CDS sequence)	XM_005204079.3 , XM_005204079.4
Accession Version	XM_005204079.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vascular cell adhesion molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103868.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGA AGATGTTTGT GATCTTTGGA GCCTCAGATG TACTTTGGAT GGTGTTTGCA 
GTTTCTCAAG CTTCCCAAAT CGACATATTC CCAAGTGAAT CCAAAATTTT TGCGCAGATT 
GGTGACTCTG TTTCACTGAC TTGCAGCACA ACAGGCTGTG AGTCCCCATC ATTGTCTTGG 
AGAACTCAGA TTGACAGTCC ACTGAATGGA AAGGTGAAAA CTGAGGGGAC CACATCCATG 
CTGATCATGG ATCCTGTTAG TTTCAAGGAT GAACACCAGT ACCTGTGCAC AGCGATTTGC 
AAGGATAAGA AACTGGAGAA AGCAATCCGA GTGGAGATCT ACTCTTTCTC TAAGGATCCA 
GAGATTCATT TGAGTAGCCC CCCAGAGGTC GGGAAGCCAG TCACAGTCAC ATGTTCGGTG 
CCTGATGTTT ACCCATTTGA AAGGCTGGAG ATAGAGTTGT TGAAAGGCAA CCGTCCCATG 
AAAGTACAGG ACTTTCTGGA GCCTTCAGAA AAAAAGGCCC AGGAAACAAA GAGCTTGGAC 
GTGACCTTCT CGCCTACTGA TGAGGATATT GGGACAGCTC TTGTTTGTCA AGCTACATTA 
CACATTTATG ATGATGATTC TCCACCCAAA GTAAGGAAAA CTACAAAAGA ACTGGAAGTC 
TACATCTCCC CCAAAGACAC GGCTATATCC GTGAATCCCT CCACAAGGCT GCAGGAAGGC 
GACTCCGTGA CGATGACGTG TGCCAGTGCA GGTTTACCAG CTCCACGGAT TCTCTGGAGC 
AAGAAATTAG ACAATGGGAA CCGACAGCTC CTTTCTGAGA ATGCAACTCT CACTTTAATT 
TCTATGAGGG CAGAAGATTC TGGGATTTAT GTGTGTGAAG GAAATAACCC TGTTGGGAAA 
GACAGAAAAG AGGTGAAATT AACTGTTCAA GAGAAAAACT TTACTGTTGA GATCTCTCCT 
GGACCCCAGA TTGCTGCTCA GGTTGGCGAC TCAGTCGTGT TGACCTGTGA TGTCAGGGAC 
TGCGAGTCCC CATCTTTCTC TTGGAGAACA CTGATAGACA GCCCTCTGAA TGGGAATGTG 
AGGAGTGAGG GCTCCAAGTC CACGTTGACC CTGAGCCCAG TGAGTTTTGA GAACGAACAT 
TTCTACCTGT GCACTGTGAT GTGTGGGCAA AAGAAACTGG AAAAGAGAAT CCAGGTGAAG 
CCCTACTCCT TCCCTAGCAA TCCAGAAATT GAAATGAGTG GTCCATTAGT GAGTGGAAAC 
CCAGTCACTG TAAGCTGCAG GGTTCCTAAT GTGTATCCTT CTGACCAGCT GGAGATTGAA 
TTGTTTAAAG GGGAGAGTAT TATGATGAAT AAAACCTTCT CGAAAGATGT AAGTAAGAAA 
GCCCTAGAGA CCAAGAGTTT GGAGAAGACC TTCATCCCCA CCACTGAAGA TATTGGAAAT 
GTTCTTGTTT GTCTGGCTCG GTTACCAATT GATGAAATGG AATTTGAACT CAAACAAAGG 
CAGAGTACAC AAACACTTTA TGTTAATGTT GCTCCCAGGG ATACAACCAT CTTGGTCAGT 
CCCTCCTCCA TCCTGGAGGA AGGTAGTTCT GTGAATATGA CATGCTCTAG CAATGGCCTT 
CCAGCTCCCA AAATCTTGTG GAGCAGGCAG CTAAGTAATG GGGATCTACA GCCTATTTCT 
GAGAATGCAA CTCTCAGCTT CATGTCTACA AAAATGGAAG ATTCTGGTAT TTATGTCTGT 
GAAGGCATTA ACCAGGTTGG AACAAGCAGA AAAGAAGTCA ACTTAATTAT CCAAGGAAGA 
GAAAGTAACA AGGACTATTT TTCTCCTGAA CTTCTGGTGC TCTATTGTGC ATCCTCCTTA 
ATAATACCTG CCATTGGAAT GATTATTTAC TTTGCTAGAA GAGCCAACAT GACAGGGTCA 
TACAGTCTTG TAGAAGCACA GAAAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204136.1
CDS	148..2100
Translation	MLRKMFVIFGASDVLWMVFAVSQASQIDIFPSESKIFAQIGDSVSLTCSTTGCESPSLSWRTQIDSPLNGKVKTEGTTSMLIMDPVSFKDEHQYLCTAICKDKKLEKAIRVEIYSFSKDPEIHLSSPPEVGKPVTVTCSVPDVYPFERLEIELLKGNRPMKVQDFLEPSEKKAQETKSLDVTFSPTDEDIGTALVCQATLHIYDDDSPPKVRKTTKELEVYISPKDTAISVNPSTRLQEGDSVTMTCASAGLPAPRILWSKKLDNGNRQLLSENATLTLISMRAEDSGIYVCEGNNPVGKDRKEVKLTVQEKNFTVEISPGPQIAAQVGDSVVLTCDVRDCESPSFSWRTLIDSPLNGNVRSEGSKSTLTLSPVSFENEHFYLCTVMCGQKKLEKRIQVKPYSFPSNPEIEMSGPLVSGNPVTVSCRVPNVYPSDQLEIELFKGESIMMNKTFSKDVSKKALETKSLEKTFIPTTEDIGNVLVCLARLPIDEMEFELKQRQSTQTLYVNVAPRDTTILVSPSSILEEGSSVNMTCSSNGLPAPKILWSRQLSNGDLQPISENATLSFMSTKMEDSGIYVCEGINQVGTSRKEVNLIIQGRESNKDYFSPELLVLYCASSLIIPAIGMIIYFARRANMTGSYSLVEAQKVC

Target ORF information:

RefSeq Version	XM_005204079.3
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular cell adhesion molecule 1 (VCAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204079.3

ORF Insert Sequence:

ATGCTTAGGA AGATGTTTGT GATCTTTGGA GCCTCAGATG TACTTTGGAT GGTGTTTGCA 
GTTTCTCAAG CTTCCCAAAT CGACATATTC CCAAGTGAAT CCAAAATTTT TGCGCAGATT 
GGTGACTCTG TTTCACTGAC TTGCAGCACA ACAGGCTGTG AGTCCCCATC ATTGTCTTGG 
AGAACTCAGA TTGACAGTCC ACTGAATGGA AAGGTGAAAA CTGAGGGGAC CACATCCATG 
CTGATCATGG ATCCTGTTAG TTTCAAGGAT GAACACCAGT ACCTGTGCAC AGCGATTTGC 
AAGGATAAGA AACTGGAGAA AGCAATCCGA GTGGAGATCT ACTCTTTCTC TAAGGATCCA 
GAGATTCATT TGAGTAGCCC CCCAGAGGTC GGGAAGCCAG TCACAGTCAC ATGTTCGGTG 
CCTGATGTTT ACCCATTTGA AAGGCTGGAG ATAGAGTTGT TGAAAGGCAA CCGTCCCATG 
AAAGTACAGG ACTTTCTGGA GCCTTCAGAA AAAAAGGCCC AGGAAACAAA GAGCTTGGAC 
GTGACCTTCT CGCCTACTGA TGAGGATATT GGGACAGCTC TTGTTTGTCA AGCTACATTA 
CACATTTATG ATGATGATTC TCCACCCAAA GTAAGGAAAA CTACAAAAGA ACTGGAAGTC 
TACATCTCCC CCAAAGACAC GGCTATATCC GTGAATCCCT CCACAAGGCT GCAGGAAGGC 
GACTCCGTGA CGATGACGTG TGCCAGTGCA GGTTTACCAG CTCCACGGAT TCTCTGGAGC 
AAGAAATTAG ACAATGGGAA CCGACAGCTC CTTTCTGAGA ATGCAACTCT CACTTTAATT 
TCTATGAGGG CAGAAGATTC TGGGATTTAT GTGTGTGAAG GAAATAACCC TGTTGGGAAA 
GACAGAAAAG AGGTGAAATT AACTGTTCAA GAGAAAAACT TTACTGTTGA GATCTCTCCT 
GGACCCCAGA TTGCTGCTCA GGTTGGCGAC TCAGTCGTGT TGACCTGTGA TGTCAGGGAC 
TGCGAGTCCC CATCTTTCTC TTGGAGAACA CTGATAGACA GCCCTCTGAA TGGGAATGTG 
AGGAGTGAGG GCTCCAAGTC CACGTTGACC CTGAGCCCAG TGAGTTTTGA GAACGAACAT 
TTCTACCTGT GCACTGTGAT GTGTGGGCAA AAGAAACTGG AAAAGAGAAT CCAGGTGAAG 
CCCTACTCCT TCCCTAGCAA TCCAGAAATT GAAATGAGTG GTCCATTAGT GAGTGGAAAC 
CCAGTCACTG TAAGCTGCAG GGTTCCTAAT GTGTATCCTT CTGACCAGCT GGAGATTGAA 
TTGTTTAAAG GGGAGAGTAT TATGATGAAT AAAACCTTCT CGAAAGATGT AAGTAAGAAA 
GCCCTAGAGA CCAAGAGTTT GGAGAAGACC TTCATCCCCA CCACTGAAGA TATTGGAAAT 
GTTCTTGTTT GTCTGGCTCG GTTACCAATT GATGAAATGG AATTTGAACT CAAACAAAGG 
CAGAGTACAC AAACACTTTA TGTTAATGTT GCTCCCAGGG ATACAACCAT CTTGGTCAGT 
CCCTCCTCCA TCCTGGAGGA AGGTAGTTCT GTGAATATGA CATGCTCTAG CAATGGCCTT 
CCAGCTCCCA AAATCTTGTG GAGCAGGCAG CTAAGTAATG GGGATCTACA GCCTATTTCT 
GAGAATGCAA CTCTCAGCTT CATGTCTACA AAAATGGAAG ATTCTGGTAT TTATGTCTGT 
GAAGGCATTA ACCAGGTTGG AACAAGCAGA AAAGAAGTCA ACTTAATTAT CCAAGGAAGA 
GAAAGTAACA AGGACTATTT TTCTCCTGAA CTTCTGGTGC TCTATTGTGC ATCCTCCTTA 
ATAATACCTG CCATTGGAAT GATTATTTAC TTTGCTAGAA GAGCCAACAT GACAGGGTCA 
TACAGTCTTG TAGAAGCACA GAAAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAM1 ( XM_005204079.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204136.1 Bos taurus vascular cell adhesion molecule 1 (VCAM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa17566
Clone ID Related Accession (Same CDS sequence)	XM_005204079.3 , XM_005204079.4
Accession Version	XM_005204079.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vascular cell adhesion molecule 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204079.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGA AGATGTTTGT GATCTTTGGA GCCTCAGATG TACTTTGGAT GGTGTTTGCA 
GTTTCTCAAG CTTCCCAAAT CGACATATTC CCAAGTGAAT CCAAAATTTT TGCGCAGATT 
GGTGACTCTG TTTCACTGAC TTGCAGCACA ACAGGCTGTG AGTCCCCATC ATTGTCTTGG 
AGAACTCAGA TTGACAGTCC ACTGAATGGA AAGGTGAAAA CTGAGGGGAC CACATCCATG 
CTGATCATGG ATCCTGTTAG TTTCAAGGAT GAACACCAGT ACCTGTGCAC AGCGATTTGC 
AAGGATAAGA AACTGGAGAA AGCAATCCGA GTGGAGATCT ACTCTTTCTC TAAGGATCCA 
GAGATTCATT TGAGTAGCCC CCCAGAGGTC GGGAAGCCAG TCACAGTCAC ATGTTCGGTG 
CCTGATGTTT ACCCATTTGA AAGGCTGGAG ATAGAGTTGT TGAAAGGCAA CCGTCCCATG 
AAAGTACAGG ACTTTCTGGA GCCTTCAGAA AAAAAGGCCC AGGAAACAAA GAGCTTGGAC 
GTGACCTTCT CGCCTACTGA TGAGGATATT GGGACAGCTC TTGTTTGTCA AGCTACATTA 
CACATTTATG ATGATGATTC TCCACCCAAA GTAAGGAAAA CTACAAAAGA ACTGGAAGTC 
TACATCTCCC CCAAAGACAC GGCTATATCC GTGAATCCCT CCACAAGGCT GCAGGAAGGC 
GACTCCGTGA CGATGACGTG TGCCAGTGCA GGTTTACCAG CTCCACGGAT TCTCTGGAGC 
AAGAAATTAG ACAATGGGAA CCGACAGCTC CTTTCTGAGA ATGCAACTCT CACTTTAATT 
TCTATGAGGG CAGAAGATTC TGGGATTTAT GTGTGTGAAG GAAATAACCC TGTTGGGAAA 
GACAGAAAAG AGGTGAAATT AACTGTTCAA GAGAAAAACT TTACTGTTGA GATCTCTCCT 
GGACCCCAGA TTGCTGCTCA GGTTGGCGAC TCAGTCGTGT TGACCTGTGA TGTCAGGGAC 
TGCGAGTCCC CATCTTTCTC TTGGAGAACA CTGATAGACA GCCCTCTGAA TGGGAATGTG 
AGGAGTGAGG GCTCCAAGTC CACGTTGACC CTGAGCCCAG TGAGTTTTGA GAACGAACAT 
TTCTACCTGT GCACTGTGAT GTGTGGGCAA AAGAAACTGG AAAAGAGAAT CCAGGTGAAG 
CCCTACTCCT TCCCTAGCAA TCCAGAAATT GAAATGAGTG GTCCATTAGT GAGTGGAAAC 
CCAGTCACTG TAAGCTGCAG GGTTCCTAAT GTGTATCCTT CTGACCAGCT GGAGATTGAA 
TTGTTTAAAG GGGAGAGTAT TATGATGAAT AAAACCTTCT CGAAAGATGT AAGTAAGAAA 
GCCCTAGAGA CCAAGAGTTT GGAGAAGACC TTCATCCCCA CCACTGAAGA TATTGGAAAT 
GTTCTTGTTT GTCTGGCTCG GTTACCAATT GATGAAATGG AATTTGAACT CAAACAAAGG 
CAGAGTACAC AAACACTTTA TGTTAATGTT GCTCCCAGGG ATACAACCAT CTTGGTCAGT 
CCCTCCTCCA TCCTGGAGGA AGGTAGTTCT GTGAATATGA CATGCTCTAG CAATGGCCTT 
CCAGCTCCCA AAATCTTGTG GAGCAGGCAG CTAAGTAATG GGGATCTACA GCCTATTTCT 
GAGAATGCAA CTCTCAGCTT CATGTCTACA AAAATGGAAG ATTCTGGTAT TTATGTCTGT 
GAAGGCATTA ACCAGGTTGG AACAAGCAGA AAAGAAGTCA ACTTAATTAT CCAAGGAAGA 
GAAAGTAACA AGGACTATTT TTCTCCTGAA CTTCTGGTGC TCTATTGTGC ATCCTCCTTA 
ATAATACCTG CCATTGGAAT GATTATTTAC TTTGCTAGAA GAGCCAACAT GACAGGGTCA 
TACAGTCTTG TAGAAGCACA GAAAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204136.1
CDS	114..2066
Translation	MLRKMFVIFGASDVLWMVFAVSQASQIDIFPSESKIFAQIGDSVSLTCSTTGCESPSLSWRTQIDSPLNGKVKTEGTTSMLIMDPVSFKDEHQYLCTAICKDKKLEKAIRVEIYSFSKDPEIHLSSPPEVGKPVTVTCSVPDVYPFERLEIELLKGNRPMKVQDFLEPSEKKAQETKSLDVTFSPTDEDIGTALVCQATLHIYDDDSPPKVRKTTKELEVYISPKDTAISVNPSTRLQEGDSVTMTCASAGLPAPRILWSKKLDNGNRQLLSENATLTLISMRAEDSGIYVCEGNNPVGKDRKEVKLTVQEKNFTVEISPGPQIAAQVGDSVVLTCDVRDCESPSFSWRTLIDSPLNGNVRSEGSKSTLTLSPVSFENEHFYLCTVMCGQKKLEKRIQVKPYSFPSNPEIEMSGPLVSGNPVTVSCRVPNVYPSDQLEIELFKGESIMMNKTFSKDVSKKALETKSLEKTFIPTTEDIGNVLVCLARLPIDEMEFELKQRQSTQTLYVNVAPRDTTILVSPSSILEEGSSVNMTCSSNGLPAPKILWSRQLSNGDLQPISENATLSFMSTKMEDSGIYVCEGINQVGTSRKEVNLIIQGRESNKDYFSPELLVLYCASSLIIPAIGMIIYFARRANMTGSYSLVEAQKVC

Target ORF information:

RefSeq Version	XM_005204079.4
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular cell adhesion molecule 1 (VCAM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204079.4

ORF Insert Sequence:

ATGCTTAGGA AGATGTTTGT GATCTTTGGA GCCTCAGATG TACTTTGGAT GGTGTTTGCA 
GTTTCTCAAG CTTCCCAAAT CGACATATTC CCAAGTGAAT CCAAAATTTT TGCGCAGATT 
GGTGACTCTG TTTCACTGAC TTGCAGCACA ACAGGCTGTG AGTCCCCATC ATTGTCTTGG 
AGAACTCAGA TTGACAGTCC ACTGAATGGA AAGGTGAAAA CTGAGGGGAC CACATCCATG 
CTGATCATGG ATCCTGTTAG TTTCAAGGAT GAACACCAGT ACCTGTGCAC AGCGATTTGC 
AAGGATAAGA AACTGGAGAA AGCAATCCGA GTGGAGATCT ACTCTTTCTC TAAGGATCCA 
GAGATTCATT TGAGTAGCCC CCCAGAGGTC GGGAAGCCAG TCACAGTCAC ATGTTCGGTG 
CCTGATGTTT ACCCATTTGA AAGGCTGGAG ATAGAGTTGT TGAAAGGCAA CCGTCCCATG 
AAAGTACAGG ACTTTCTGGA GCCTTCAGAA AAAAAGGCCC AGGAAACAAA GAGCTTGGAC 
GTGACCTTCT CGCCTACTGA TGAGGATATT GGGACAGCTC TTGTTTGTCA AGCTACATTA 
CACATTTATG ATGATGATTC TCCACCCAAA GTAAGGAAAA CTACAAAAGA ACTGGAAGTC 
TACATCTCCC CCAAAGACAC GGCTATATCC GTGAATCCCT CCACAAGGCT GCAGGAAGGC 
GACTCCGTGA CGATGACGTG TGCCAGTGCA GGTTTACCAG CTCCACGGAT TCTCTGGAGC 
AAGAAATTAG ACAATGGGAA CCGACAGCTC CTTTCTGAGA ATGCAACTCT CACTTTAATT 
TCTATGAGGG CAGAAGATTC TGGGATTTAT GTGTGTGAAG GAAATAACCC TGTTGGGAAA 
GACAGAAAAG AGGTGAAATT AACTGTTCAA GAGAAAAACT TTACTGTTGA GATCTCTCCT 
GGACCCCAGA TTGCTGCTCA GGTTGGCGAC TCAGTCGTGT TGACCTGTGA TGTCAGGGAC 
TGCGAGTCCC CATCTTTCTC TTGGAGAACA CTGATAGACA GCCCTCTGAA TGGGAATGTG 
AGGAGTGAGG GCTCCAAGTC CACGTTGACC CTGAGCCCAG TGAGTTTTGA GAACGAACAT 
TTCTACCTGT GCACTGTGAT GTGTGGGCAA AAGAAACTGG AAAAGAGAAT CCAGGTGAAG 
CCCTACTCCT TCCCTAGCAA TCCAGAAATT GAAATGAGTG GTCCATTAGT GAGTGGAAAC 
CCAGTCACTG TAAGCTGCAG GGTTCCTAAT GTGTATCCTT CTGACCAGCT GGAGATTGAA 
TTGTTTAAAG GGGAGAGTAT TATGATGAAT AAAACCTTCT CGAAAGATGT AAGTAAGAAA 
GCCCTAGAGA CCAAGAGTTT GGAGAAGACC TTCATCCCCA CCACTGAAGA TATTGGAAAT 
GTTCTTGTTT GTCTGGCTCG GTTACCAATT GATGAAATGG AATTTGAACT CAAACAAAGG 
CAGAGTACAC AAACACTTTA TGTTAATGTT GCTCCCAGGG ATACAACCAT CTTGGTCAGT 
CCCTCCTCCA TCCTGGAGGA AGGTAGTTCT GTGAATATGA CATGCTCTAG CAATGGCCTT 
CCAGCTCCCA AAATCTTGTG GAGCAGGCAG CTAAGTAATG GGGATCTACA GCCTATTTCT 
GAGAATGCAA CTCTCAGCTT CATGTCTACA AAAATGGAAG ATTCTGGTAT TTATGTCTGT 
GAAGGCATTA ACCAGGTTGG AACAAGCAGA AAAGAAGTCA ACTTAATTAT CCAAGGAAGA 
GAAAGTAACA AGGACTATTT TTCTCCTGAA CTTCTGGTGC TCTATTGTGC ATCCTCCTTA 
ATAATACCTG CCATTGGAAT GATTATTTAC TTTGCTAGAA GAGCCAACAT GACAGGGTCA 
TACAGTCTTG TAGAAGCACA GAAAGTCTGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAM1 ( NM_174484.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776909.1 Bos taurus vascular cell adhesion molecule 1 (VCAM1), mRNA.

Price & Availability↑

CloneID	OBa39439
Clone ID Related Accession (Same CDS sequence)	NM_174484.1
Accession Version	NM_174484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	vascular cell adhesion molecule 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB052746.1. On Mar 29, 2005 this sequence version replaced XM_611900.1. ##Evidence-Data-START## Transcript exon combination :: AB052746.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGCTTAGGA AGATGTTTGT GATCTTTGGA GCCTCAGATG TACTTTGGAT GGTGTTTGCA 
GTTTCTCAAG CTTCCCAAAT CGACATATTC CCAAGTGAAT CCAAAATTTT TGCGCAGATT 
GGTGACTCTG TTTCACTGAC TTGCAGCACA ACAGGCTGTG AGTCCCCATC ATTGTCTTGG 
AGAACTCAGA TTGACAGTCC ACTGAATGGA AAGGTGAAAA CTGAGGGGAC CACATCCATG 
CTGATCATGG ATCCTGTTAG TTTCAAGGAT GAACACCAGT ACCTGTGCAC AGCGATTTGC 
AAGGATAAGA AACTGGAGAA AGCAATCCGA GTGGAGATCT ACTCTTTCTC TAAGGATCCA 
GAGATTCATT TGAGTAGCCC CCCAGAGGTC GGGAAGCCAG TCACAGTCAC ATGTTCGGTG 
CCTGATGTTT ACCCATTTGA AAGGCTGGAG ATAGAGTTGT TGAAAGGCAA CCGTCCCATG 
AAAGTACAGG ACTTTCTGGA GCCTTCAGAA AAAAAGGCCC AGGAAACAAA GAGCTTGGAC 
GTGACCTTCT CGCCTACTGA TGAGGATATT GGGACAGCTC TTGTTTGTCA AGCTACATTA 
CACATTTATG ATGATGATTC TCCACCCAAA GTAAGGAAAA CTACAAAAGA ACTGGAAGTC 
TACATCTCCC CCAAAGACAC GGCTATATCC GTGAATCCCT CCACAAGGCT GCAGGAAGGC 
GACTCCGTGA CGATGACGTG TGCCAGTGCA GGTTTACCAG CTCCACGGAT TCTCTGGAGC 
AAGAAATTAG ACAATGGGAA CCGACAGCTC CTTTCTGAGA ATGCAACTCT CACTTTAATT 
TCTATGAGGG CAGAAGATTC TGGGATTTAT GTGTGTGAAG GAAATAACCC TGTTGGGAAA 
GACAGAAAAG AGGTGAAATT AACTGTTCAA GAGAAAAACT TTACTGTTGA GATCTCTCCT 
GGACCCCAGA TTGCTGCTCA GGTTGGCGAC TCAGTCGTGT TGACCTGTGA TGTCAGGGAC 
TGCGAGTCCC CATCTTTCTC TTGGAGAACA CTGATAGACA GCCCTCTGAA TGGGAATGTG 
AGGAGTGAGG GCTCCAAGTC CACGTTGACC CTGAGCCCAG TGAGTTTTGA GAACGAACAT 
TTCTACCTGT GCACTGTGAT GTGTGGGCAA AAGAAACTGG AAAAGAGAAT CCAGGTGAAG 
CCCTACTCCT TCCCTAGCAA TCCAGAAATT GAAATGAGTG GTCCATTAGT GAGTGGAAAC 
CCAGTCACTG TAAGCTGCAG GGTTCCTAAT GTGTATCCTT CTGACCAGCT GGAGATTGAA 
TTGTTTAAAG GGGAGAGTAT TATGATGAAT AAAACCTTCT CGAAAGATGT AAGTAAGAAA 
GCCCTAGAGA CCAAGAGTTT GGAGAAGACC TTCATCCCCA CCACTGAAGA TATTGGAAAT 
GTTCTTGTTT GTCTGGCTCG GTTACCAATT GATGAAATGG AATTTGAACT CAAACAAAGG 
CAGAGTACAC AAACACTTTA TGTTAATGTT GCTCCCAGGG ATACAACCAT CTTGGTCAGT 
CCCTCCTCCA TCCTGGAGGA AGGTAGTTCT GTGAATATGA CATGCTCTAG CAATGGCCTT 
CCAGCTCCCA AAATCTTGTG GAGCAGGCAG CTAAGTAATG GGGATCTACA GCCTATTTCT 
GAGAATGCAA CTCTCAGCTT CATGTCTACA AAAATGGAAG ATTCTGGTAT TTATGTCTGT 
GAAGGCATTA ACCAGGTTGG AACAAGCAGA AAAGAAGTCA ACTTAATTAT CCAAGTTGCT 
CCAAAAGATA TACAACTTAC AGCTTTTCCT TCTGAGAGGG TCAAGGAAGG AGACACTGTC 
ATTATCTCCT GTACGTGTGG AAATGTTCCC CCAACTTTGA TAATTCTGAA GAAAAAAGCA 
GAGACTGGCT ACACAGTGCT AAAGTCTACA GGTGGTGCAT ATACCATCCA CAGGGCTCAG 
TTAGAGGATG CGGGAGTATA CCAGTGTGAA TCTAAAAATG AAGTTGGCTC ACAGCTAAGA 
AGCCTAACAC TTGATGTTAA AGGAAGAGAA AGTAACAAGG ACTATTTTTC TCCTGAACTT 
CTGGTGCTCT ATTGTGCATC CTCCTTAATA ATACCTGCCA TTGGAATGAT TATTTACTTT 
GCTAGAAGAG CCAACATGAC AGGGTCATAC AGTCTTGTAG AAGCACAGAA AGTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776909.1
CDS	78..2297
Translation	MLRKMFVIFGASDVLWMVFAVSQASQIDIFPSESKIFAQIGDSVSLTCSTTGCESPSLSWRTQIDSPLNGKVKTEGTTSMLIMDPVSFKDEHQYLCTAICKDKKLEKAIRVEIYSFSKDPEIHLSSPPEVGKPVTVTCSVPDVYPFERLEIELLKGNRPMKVQDFLEPSEKKAQETKSLDVTFSPTDEDIGTALVCQATLHIYDDDSPPKVRKTTKELEVYISPKDTAISVNPSTRLQEGDSVTMTCASAGLPAPRILWSKKLDNGNRQLLSENATLTLISMRAEDSGIYVCEGNNPVGKDRKEVKLTVQEKNFTVEISPGPQIAAQVGDSVVLTCDVRDCESPSFSWRTLIDSPLNGNVRSEGSKSTLTLSPVSFENEHFYLCTVMCGQKKLEKRIQVKPYSFPSNPEIEMSGPLVSGNPVTVSCRVPNVYPSDQLEIELFKGESIMMNKTFSKDVSKKALETKSLEKTFIPTTEDIGNVLVCLARLPIDEMEFELKQRQSTQTLYVNVAPRDTTILVSPSSILEEGSSVNMTCSSNGLPAPKILWSRQLSNGDLQPISENATLSFMSTKMEDSGIYVCEGINQVGTSRKEVNLIIQVAPKDIQLTAFPSERVKEGDTVIISCTCGNVPPTLIILKKKAETGYTVLKSTGGAYTIHRAQLEDAGVYQCESKNEVGSQLRSLTLDVKGRESNKDYFSPELLVLYCASSLIIPAIGMIIYFARRANMTGSYSLVEAQKVC

Target ORF information:

RefSeq Version	NM_174484.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular cell adhesion molecule 1 (VCAM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174484.1

ORF Insert Sequence:

ATGCTTAGGA AGATGTTTGT GATCTTTGGA GCCTCAGATG TACTTTGGAT GGTGTTTGCA 
GTTTCTCAAG CTTCCCAAAT CGACATATTC CCAAGTGAAT CCAAAATTTT TGCGCAGATT 
GGTGACTCTG TTTCACTGAC TTGCAGCACA ACAGGCTGTG AGTCCCCATC ATTGTCTTGG 
AGAACTCAGA TTGACAGTCC ACTGAATGGA AAGGTGAAAA CTGAGGGGAC CACATCCATG 
CTGATCATGG ATCCTGTTAG TTTCAAGGAT GAACACCAGT ACCTGTGCAC AGCGATTTGC 
AAGGATAAGA AACTGGAGAA AGCAATCCGA GTGGAGATCT ACTCTTTCTC TAAGGATCCA 
GAGATTCATT TGAGTAGCCC CCCAGAGGTC GGGAAGCCAG TCACAGTCAC ATGTTCGGTG 
CCTGATGTTT ACCCATTTGA AAGGCTGGAG ATAGAGTTGT TGAAAGGCAA CCGTCCCATG 
AAAGTACAGG ACTTTCTGGA GCCTTCAGAA AAAAAGGCCC AGGAAACAAA GAGCTTGGAC 
GTGACCTTCT CGCCTACTGA TGAGGATATT GGGACAGCTC TTGTTTGTCA AGCTACATTA 
CACATTTATG ATGATGATTC TCCACCCAAA GTAAGGAAAA CTACAAAAGA ACTGGAAGTC 
TACATCTCCC CCAAAGACAC GGCTATATCC GTGAATCCCT CCACAAGGCT GCAGGAAGGC 
GACTCCGTGA CGATGACGTG TGCCAGTGCA GGTTTACCAG CTCCACGGAT TCTCTGGAGC 
AAGAAATTAG ACAATGGGAA CCGACAGCTC CTTTCTGAGA ATGCAACTCT CACTTTAATT 
TCTATGAGGG CAGAAGATTC TGGGATTTAT GTGTGTGAAG GAAATAACCC TGTTGGGAAA 
GACAGAAAAG AGGTGAAATT AACTGTTCAA GAGAAAAACT TTACTGTTGA GATCTCTCCT 
GGACCCCAGA TTGCTGCTCA GGTTGGCGAC TCAGTCGTGT TGACCTGTGA TGTCAGGGAC 
TGCGAGTCCC CATCTTTCTC TTGGAGAACA CTGATAGACA GCCCTCTGAA TGGGAATGTG 
AGGAGTGAGG GCTCCAAGTC CACGTTGACC CTGAGCCCAG TGAGTTTTGA GAACGAACAT 
TTCTACCTGT GCACTGTGAT GTGTGGGCAA AAGAAACTGG AAAAGAGAAT CCAGGTGAAG 
CCCTACTCCT TCCCTAGCAA TCCAGAAATT GAAATGAGTG GTCCATTAGT GAGTGGAAAC 
CCAGTCACTG TAAGCTGCAG GGTTCCTAAT GTGTATCCTT CTGACCAGCT GGAGATTGAA 
TTGTTTAAAG GGGAGAGTAT TATGATGAAT AAAACCTTCT CGAAAGATGT AAGTAAGAAA 
GCCCTAGAGA CCAAGAGTTT GGAGAAGACC TTCATCCCCA CCACTGAAGA TATTGGAAAT 
GTTCTTGTTT GTCTGGCTCG GTTACCAATT GATGAAATGG AATTTGAACT CAAACAAAGG 
CAGAGTACAC AAACACTTTA TGTTAATGTT GCTCCCAGGG ATACAACCAT CTTGGTCAGT 
CCCTCCTCCA TCCTGGAGGA AGGTAGTTCT GTGAATATGA CATGCTCTAG CAATGGCCTT 
CCAGCTCCCA AAATCTTGTG GAGCAGGCAG CTAAGTAATG GGGATCTACA GCCTATTTCT 
GAGAATGCAA CTCTCAGCTT CATGTCTACA AAAATGGAAG ATTCTGGTAT TTATGTCTGT 
GAAGGCATTA ACCAGGTTGG AACAAGCAGA AAAGAAGTCA ACTTAATTAT CCAAGTTGCT 
CCAAAAGATA TACAACTTAC AGCTTTTCCT TCTGAGAGGG TCAAGGAAGG AGACACTGTC 
ATTATCTCCT GTACGTGTGG AAATGTTCCC CCAACTTTGA TAATTCTGAA GAAAAAAGCA 
GAGACTGGCT ACACAGTGCT AAAGTCTACA GGTGGTGCAT ATACCATCCA CAGGGCTCAG 
TTAGAGGATG CGGGAGTATA CCAGTGTGAA TCTAAAAATG AAGTTGGCTC ACAGCTAAGA 
AGCCTAACAC TTGATGTTAA AGGAAGAGAA AGTAACAAGG ACTATTTTTC TCCTGAACTT 
CTGGTGCTCT ATTGTGCATC CTCCTTAATA ATACCTGCCA TTGGAATGAT TATTTACTTT 
GCTAGAAGAG CCAACATGAC AGGGTCATAC AGTCTTGTAG AAGCACAGAA AGTCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase A-alpha directly phosphorylates FoxO1 in vascular endothelial cells to regulate expression of vascular cellular adhesion molecule-1 mRNA.
The Journal of biological chemistry286(8)6423-32(2011 Feb)
Lee JW,Chen H,Pullikotil P,Quon MJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

CAMP-response element-binding protein mediates tumor necrosis factor-alpha-induced vascular cell adhesion molecule-1 expression in endothelial cells.
Hypertension research : official journal of the Japanese Society of Hypertension29(1)39-47(2006 Jan)
Ono H,Ichiki T,Ohtsubo H,Fukuyama K,Imayama I,Iino N,Masuda S,Hashiguchi Y,Takeshita A,Sunagawa K

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VCAM1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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