TRPC4 cDNA ORF clone, Bos taurus(cattle)

The following TRPC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa24616 NM_174478.2
Latest version!
Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_015473752.2
Latest version!
Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_024999784.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_015473750.2
Latest version!
Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_015473751.2
Latest version!
Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_005213554.4
Latest version!
Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_010810662.3
Latest version!
Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OBa24616 XM_015473752.1 Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_005213554.3 Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_010810662.2 Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_015473750.1 Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24616 XM_015473751.1 Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version NM_174478.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X99792.1. On or before Mar 29, 2005 this sequence version replaced XM_580823.1, NM_174478.1. ##Evidence-Data-START## Transcript exon combination :: X99792.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145415, SAMN03145437 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776903.1
    CDS262..3207
    Translation

    Target ORF information:

    RefSeq Version NM_174478.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174478.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_015473752.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329238.1
    CDS501..3446
    Translation

    Target ORF information:

    RefSeq Version XM_015473752.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473752.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_024999784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855552.1
    CDS1176..4121
    Translation

    Target ORF information:

    RefSeq Version XM_024999784.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_015473750.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329236.1
    CDS106..3051
    Translation

    Target ORF information:

    RefSeq Version XM_015473750.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_015473751.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329237.1
    CDS571..3516
    Translation

    Target ORF information:

    RefSeq Version XM_015473751.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_015473752.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329238.1
    CDS516..3461
    Translation

    Target ORF information:

    RefSeq Version XM_015473752.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_005213554.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213554.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213611.1
    CDS557..3502
    Translation

    Target ORF information:

    RefSeq Version XM_005213554.4
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213554.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_005213554.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213611.1
    CDS576..3521
    Translation

    Target ORF information:

    RefSeq Version XM_005213554.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213554.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_010810662.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808964.1
    CDS329..3274
    Translation

    Target ORF information:

    RefSeq Version XM_010810662.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810662.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_010810662.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808964.1
    CDS310..3255
    Translation

    Target ORF information:

    RefSeq Version XM_010810662.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810662.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_015473750.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329236.1
    CDS1027..3972
    Translation

    Target ORF information:

    RefSeq Version XM_015473750.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473750.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24616
    Clone ID Related Accession (Same CDS sequence) XM_015473752.2 , NM_174478.2 , XM_024999784.1 , XM_015473750.1 , XM_015473751.1 , XM_015473752.1 , XM_005213554.4 , XM_005213554.3 , XM_010810662.2 , XM_010810662.3 , XM_015473750.2 , XM_015473751.2
    Accession Version XM_015473751.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product short transient receptor potential channel 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473751.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329237.1
    CDS556..3501
    Translation

    Target ORF information:

    RefSeq Version XM_015473751.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily C member 4 (TRPC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473751.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACATGG CGCAGTTCTA TTACAAAAGA AATGTTAACG CCCCCTATAG AGACCGCATC 
    CCTCTCAGGA TAGTCCGAGC AGAATCCGAA CTCTCACCTT CCGAGAAAGC CTACCTAAAT
    GCTGTGGAAA AGGGAGATTA TGCAAGCGTC AAGAAATCCC TAGAAGAAGC TGAAATTTAT
    TTTAAAATCA ATATTAATTG CATCGATCCT CTTGGAAGAA CTGCTCTTCT CATTGCAATT
    GAAAATGAGA ACTTGGAACT CATTGAACTC CTCTTAAGCT TTAACGTCTA TGTTGGAGAC
    GCCCTATTAC ATGCTATCAG GAAAGAAGTT GTGGGAGCTG TCGAGCTGTT GCTGAACCAC
    AAAAAGCCCA GTGGAGAAAA ACAGGTGCCT CCCATACTCC TTGATAAGCA GTTCTCTGAA
    TTCACTCCAG ACATTACACC CATCATTTTG GCAGCCCATA CAAATAATTA TGAGATAATA
    AAACTTTTGG TTCAGAAAGG CGTCTCAGTG CCCAGACCCC ACGAGGTCCG CTGTAACTGC
    GTTGAATGTG TGTCTAGCTC AGATGTGGAC AGTCTCCGCC ACTCTCGCTC CAGACTCAAC
    ATCTACAAGG CCCTGGCCAG CCCGTCTCTC ATTGCCCTGT CCAGTGAAGA CCCTTTTCTC
    ACAGCCTTTC AGCTGAGTTG GGAGCTCCAG GAACTGAGTA AGGTGGAAAA TGAATTCAAG
    TCAGAATATG AGGAGCTGTC TCGACAGTGC AAACAATTTG CCAAGGACCT ACTGGATCAG
    ACAAGAAGTT CCCGAGAGCT GGAAATCATT CTTAATTACC GTGATGACAA CAGTCTCCTG
    GAAGAACAAA GTGGAAACGA TCTCGCGAGA CTCAAATTGG CCATTAAGTA CCGTCAGAAA
    GAGTTCGTGG CTCAGCCCAA CTGTCAACAA CTGCTGGCGT CTCGCTGGTA CGACGAGTTC
    CCCGGCTGGA GGAGACGACA CTGGGCCGTG AAGATGGTGA CGTGTTTCAT AGTAGGACTC
    CTTTTCCCCG TCTTCTCTGT GTGCTACCTT ATAGCTCCCA AAAGCCCCCT CGGACTCTTC
    ATCCGAAAGC CATTTATCAA GTTCATCTGC CACACAGCGT CCTATTTGAC TTTTCTGTTC
    CTGCTCCTGC TTGCCTCTCA GCACATCGAC AGGTCAGACT TGAACAGGCA AGGTCCACCA
    CCAACCATCG TCGAGTGGAT GATATTACCG TGGGTCCTGG GTTTTATATG GGGAGAGATT
    AAACAAATGT GGGATGGTGG ACTTCAAGAT TACATCCATG ATTGGTGGAA TTTAATGGAT
    TTTGTGATGA ACTCCTTATA CCTAGCAACA ATCTCCTTGA AAATCGTTGC ATTTGTAAAG
    TACAGTGCTC TTAATCCACG TGAATCTTGG GACATGTGGC ACCCCACGCT GGTGGCTGAA
    GCTTTGTTTG CTATTGCAAA TATATTCAGT TCCCTGCGTC TGATCTCACT GTTCACTGCA
    AATTCTCACC TGGGGCCTCT GCAGATATCT CTGGGAAGAA TGCTCCTGGA CATCTTGAAG
    TTTCTGTTCA TCTACTGCCT CGTGCTGCTA GCATTTGCCA ATGGCCTAAA CCAGTTGTAC
    TTCTATTATG AAGAAACAAA AGGGTTAAGC TGCAAAGGCA TACGATGTGA AAAGCAGAAT
    AACGCCTTTT CAACGTTATT TGAGACACTC CAGTCCCTGT TTTGGTCAAT ATTTGGGCTC
    ATCAACTTAT ATGTGACCAA TGTCAAAGCA CAGCATGAAT TCACCGAATT TGTTGGTGCC
    ACCATGTTTG GGACATACAA CGTCATCTCT CTGGTTGTCC TGCTTAACAT GTTAATAGCT
    ATGATGAATA ATTCTTACCA ACTTATTGCT GACCATGCGG ATATAGAATG GAAATTTGCT
    AGAACAAAGC TTTGGATGAG TTATTTTGAA GAAGGAGGTA CCCTTCCTAC CCCCTTCAAT
    GTCATCCCGA GCCCCAAGTC TCTCTGGTAC CTGATCAAAT GGATATGGAC ACACCTGTGC
    AAGAAAAAGA TGAGAAGAAA GCCAGAAAGT TTTGGAACGA TAGGGAGGCG AGCTGCTGAT
    AATTTGAGAA GACATCACCA ATATCAAGAA GTTATGCGGA ACCTGGTAAA GCGATACGTT
    GCCGCAATGA TTAGAGATGC CAAAACTGAG GAAGGTCTGA CAGAGGAGAA CTTTAAGGAA
    CTCAAGCAAG ACATTTCTAG CTTCCGCTTT GAAGTCCTGG GGTTATTAAG AGGAAGCAAA
    CTCTCCACGG TCCAGTCTGC CCAGGGCACC AAGGAGTCTT CTAACTCCGC AGACTCGGAT
    GAAAAGAGTG ATAACGAAGG TAGCAGCAAG GACAAGAAAA AGAACTTCAG CCTCTTCGAC
    CTGACCACCC TCATTCACCC GCGGTCGGCG GCAATTGCCG CTGAAAGACA CACCATCAGC
    AATGGCTCCG CCCTCGTGGT GCAGGAGCCC CCGAGGGAGA AGCAGAGAAA AGTGAATTTT
    GTGACCGATA TCAGACACTT TGGATTGTTT CACAGACGAT CGAAACAACA CGCCGCCGCC
    GAGCAAAACG CAAACCAGAT CTTCTCCGTT TCAGAAGGGG TGGCCCGCCA ACAAGCTGAA
    GGACCCCTGG AGAGGAGCAT CCAGCTGGAG TCCCGAACTC TAGCTTCACG GGGTGACTTG
    AACATTCCGG GGCTCAGCGA ACAGTGTATA TTGGTAGATC ATCGAGAAAG AAATACAGAC
    TCCCTTGGTG TACAGGTGAG CAAGAGAGTG TGTTCATTCA AGTCAGAGAA GGTGGTGGTG
    GAGGACACGG TTCCCATCAT ACCAAAGGAG AAGAAACACG CGAAAGAGGA GGACTCGAGT
    GCAGACTATG ATGCAAACCT CACAGACACC ATCACCCACG AAGACTATGT GACCACAAGG
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.