TIMP1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMP1
Entrez Gene ID	282092
Full Name	TIMP metallopeptidase inhibitor 1
General protein information	Preferred Names TIMP metallopeptidase inhibitor 1 Names metalloproteinase inhibitor 1 EG-1 TIMP-1 embryogenin-1 tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) tissue inhibitor of metalloproteinases 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174471.3	NP_776896.1	metalloproteinase inhibitor 1 precursor
XM_005228099.1	XP_005228156.1	metalloproteinase inhibitor 1 isoform X1
XM_005228099.2	XP_005228156.1	metalloproteinase inhibitor 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Timp1	NP_446271.1
	TIMP1	NP_001028111.1
Mus musculus (house mouse)	Timp1	NP_035723.2
Canis lupus familiaris (dog)	TIMP1	NP_001003182.1
Bos taurus (cattle)	TIMP1	NP_776896.1
Homo sapiens (human)	TIMP1	NP_003245.1

Gene Ontology
Bibliography

Related articles in PubMed

Decreased expression of matrix metalloproteinase-9 and increased expression of tissue inhibitors of matrix metalloproteinase-1 in paratuberculosis-infected cattle in the ELISA-negative subclinical stage.
Wang X, Wang H, Aodon-geril, Shu Y, Momotani Y, Nagata R, Mori Y, Momotani E
Animal biotechnology22(1)44-9(2011 Jan)

Epidermal growth factor (EGF) induces motility and upregulates MMP-9 and TIMP-1 in bovine trophoblast cells.
Dilly M, Hambruch N, Haeger JD, Pfarrer C
Molecular reproduction and development77(7)622-9(2010 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Inhibitory activity on matrix metalloproteinases and cell-proliferating activity of tissue inhibitor of metalloproteinases-1 (TIMP-1)-contrastive difference between human and bovine TIMP-1s on mouse cell proliferation.
Nishimura K, Yamashita K, Kato Y, Iwata T, Iwata K, Nishikawa T, Goto S, Hayakawa T
Growth factors (Chur, Switzerland)23(2)135-42(2005 Jun)

ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells.
Reichenstein M, Reich R, LeHoux JG, Hanukoglu I
Molecular and cellular endocrinology215(1-2)109-14(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Decreased MMP-9 and increased TIMP-1 expression were found in peripheral blood cells from Mycobacterium avium subsp. paratuberculosis (Map)-infected cattle after stimulation with Map lysate and Map purified protein derivative than in control cattle.
Title: Decreased expression of matrix metalloproteinase-9 and increased expression of tissue inhibitors of matrix metalloproteinase-1 in paratuberculosis-infected cattle in the ELISA-negative subclinical stage.

We used a trophoblast cell line (F3) derived from bovine placentomes to examine the influence of EGF on MMP-9 and TIMP-1 expression by semiquantitative RT-PCR and MMP activity by zymography.
Title: Epidermal growth factor (EGF) induces motility and upregulates MMP-9 and TIMP-1 in bovine trophoblast cells.

analysis of species specificity of human and bovine TIMP-1 binding to mouse TIMP-1 receptor
Title: Inhibitory activity on matrix metalloproteinases and cell-proliferating activity of tissue inhibitor of metalloproteinases-1 (TIMP-1)-contrastive difference between human and bovine TIMP-1s on mouse cell proliferation.

Identification, purification and partial characterization of bovine pulmonary artery smooth muscle tissue inhibitor of matrix metalloproteinase-1
Title: Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in bovine pulmonary artery smooth muscle.

the different temporal expression patterns of TIMP-1 and TIMP-2 suggest that TIMP-1 may be important for luteal formation and development, while TIMP-2 may play significant roles during luteal development and maintenance
Title: Temporal and spatial expression of tissue inhibitors of metalloproteinases 1 and 2 (TIMP-1 and -2) in the bovine corpus luteum.

The following TIMP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43391	NM_174471.3 Latest version!	Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa35539	XM_005228099.2 Latest version!	Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OBa35539	XM_005228099.1	Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMP1 ( NM_174471.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776896.1 Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), mRNA.

Price & Availability↑

CloneID	OBa43391
Clone ID Related Accession (Same CDS sequence)	NM_174471.3
Accession Version	NM_174471.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	metalloproteinase inhibitor 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY295346.1. On Jun 19, 2003 this sequence version replaced NM_174471.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY295346.1, EV658395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCCT TTGCACCCAT GGCCTCTGGC ATCCTGTTGC TGCTGTGGCT CACAGCCCCC 
AGCCGAGCCT GTACCTGCGT CCCACCCCAC CCACAGACGG CCTTCTGCAA CTCCGATGTC 
GTCATCAGGG CCAAGTTCGT GGGGACCGCA GAAGTCAATG AAACTGCCTT ATACCAGCGT 
TATGAGATCA AGATGACTAA GATGTTCAAA GGGTTCAGTG CCTTGAGGGA TGCCCCTGAC 
ATCCGGTTCA TCTACACCCC TGCCATGGAG AGCGTCTGCG GATACTTCCA CAGGTCCCAG 
AACCGCAGTG AGGAGTTTCT CATAGCTGGA CAATTGTCGA ATGGGCACCT GCACATCACC 
ACCTGCAGTT TTGTGGCTCC CTGGAACAGC ATGAGTTCTG CTCAGCGCCG GGGATTCACC 
AAGACCTATG CTGCTGGTTG TGAGGAATGC ACAGTGTTTC CCTGCTCATC CATCCCCTGC 
AAACTGCAGA GCGACACTCA CTGCTTGTGG ACGGACCAGC TGCTCACAGG CTCTGACAAG 
GGGTTCCAGA GCCGCCACCT GGCCTGCCTG CCCCGGGAGC CAGGGCTGTG CACCTGGCAG 
TCCTTGCGGG CCCAGATGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776896.1
CDS	83..706
Translation	MAPFAPMASGILLLLWLTAPSRACTCVPPHPQTAFCNSDVVIRAKFVGTAEVNETALYQRYEIKMTKMFKGFSALRDAPDIRFIYTPAMESVCGYFHRSQNRSEEFLIAGQLSNGHLHITTCSFVAPWNSMSSAQRRGFTKTYAAGCEECTVFPCSSIPCKLQSDTHCLWTDQLLTGSDKGFQSRHLACLPREPGLCTWQSLRAQMA

Target ORF information:

RefSeq Version	NM_174471.3
Organism	Bos taurus(cattle)
Definition	Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174471.3

ORF Insert Sequence:

ATGGCCCCCT TTGCACCCAT GGCCTCTGGC ATCCTGTTGC TGCTGTGGCT CACAGCCCCC 
AGCCGAGCCT GTACCTGCGT CCCACCCCAC CCACAGACGG CCTTCTGCAA CTCCGATGTC 
GTCATCAGGG CCAAGTTCGT GGGGACCGCA GAAGTCAATG AAACTGCCTT ATACCAGCGT 
TATGAGATCA AGATGACTAA GATGTTCAAA GGGTTCAGTG CCTTGAGGGA TGCCCCTGAC 
ATCCGGTTCA TCTACACCCC TGCCATGGAG AGCGTCTGCG GATACTTCCA CAGGTCCCAG 
AACCGCAGTG AGGAGTTTCT CATAGCTGGA CAATTGTCGA ATGGGCACCT GCACATCACC 
ACCTGCAGTT TTGTGGCTCC CTGGAACAGC ATGAGTTCTG CTCAGCGCCG GGGATTCACC 
AAGACCTATG CTGCTGGTTG TGAGGAATGC ACAGTGTTTC CCTGCTCATC CATCCCCTGC 
AAACTGCAGA GCGACACTCA CTGCTTGTGG ACGGACCAGC TGCTCACAGG CTCTGACAAG 
GGGTTCCAGA GCCGCCACCT GGCCTGCCTG CCCCGGGAGC CAGGGCTGTG CACCTGGCAG 
TCCTTGCGGG CCCAGATGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMP1 ( XM_005228099.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228156.1 Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35539
Clone ID Related Accession (Same CDS sequence)	XM_005228099.1 , XM_005228099.2
Accession Version	XM_005228099.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	metalloproteinase inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104731.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCAG GAAGCCTGGA GGCCTGTGGT TTCCTACCGC TGCTGCTACC CCGCCCTCGC 
ATGGATATTT ATCCTCCGCA GCTCAGGCCC TGCCGCCATC GCCGCAGACC CAGCGCCCAG 
AGAGGCTACA CCAGAGAACG CACCATGGCC CCCTTTGCAC CCATGGCCTC TGGCATCCTG 
TTGCTGCTGT GGCTCACAGC CCCCAGCCGA GCCTGTACCT GCGTCCCACC CCACCCACAG 
ACGGCCTTCT GCAACTCCGA TGTCGTCATC AGGGCCAAGT TCGTGGGGAC CGCAGAAGTC 
AATGAAACTG CCTTATACCA GCGTTATGAG ATCAAGATGA CTAAGATGTT CAAAGGGTTC 
AGTGCCTTGA GGGATGCCCC TGACATCCGG TTCATCTACA CCCCTGCCAT GGAGAGCGTC 
TGCGGATACT TCCACAGGTC CCAGAACCGC AGTGAGGAGT TTCTCATAGC TGGACAATTG 
TCGAATGGGC ACCTGCACAT CACCACCTGC AGTTTTGTGG CTCCCTGGAA CAGCATGAGT 
TCTGCTCAGC GCCGGGGATT CACCAAGACC TATGCTGCTG GTTGTGAGGA ATGCACAGTG 
TTTCCCTGCT CATCCATCCC CTGCAAACTG CAGAGCGACA CTCACTGCTT GTGGACGGAC 
CAGCTGCTCA CAGGCTCTGA CAAGGGGTTC CAGAGCCGCC ACCTGGCCTG CCTGCCCCGG 
GAGCCAGGGC TGTGCACCTG GCAGTCCTTG CGGGCCCAGA TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228156.1
CDS	69..836
Translation	MHPGSLEACGFLPLLLPRPRMDIYPPQLRPCRHRRRPSAQRGYTRERTMAPFAPMASGILLLLWLTAPSRACTCVPPHPQTAFCNSDVVIRAKFVGTAEVNETALYQRYEIKMTKMFKGFSALRDAPDIRFIYTPAMESVCGYFHRSQNRSEEFLIAGQLSNGHLHITTCSFVAPWNSMSSAQRRGFTKTYAAGCEECTVFPCSSIPCKLQSDTHCLWTDQLLTGSDKGFQSRHLACLPREPGLCTWQSLRAQMA

Target ORF information:

RefSeq Version	XM_005228099.1
Organism	Bos taurus(cattle)
Definition	Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228099.1

ORF Insert Sequence:

ATGCATCCAG GAAGCCTGGA GGCCTGTGGT TTCCTACCGC TGCTGCTACC CCGCCCTCGC 
ATGGATATTT ATCCTCCGCA GCTCAGGCCC TGCCGCCATC GCCGCAGACC CAGCGCCCAG 
AGAGGCTACA CCAGAGAACG CACCATGGCC CCCTTTGCAC CCATGGCCTC TGGCATCCTG 
TTGCTGCTGT GGCTCACAGC CCCCAGCCGA GCCTGTACCT GCGTCCCACC CCACCCACAG 
ACGGCCTTCT GCAACTCCGA TGTCGTCATC AGGGCCAAGT TCGTGGGGAC CGCAGAAGTC 
AATGAAACTG CCTTATACCA GCGTTATGAG ATCAAGATGA CTAAGATGTT CAAAGGGTTC 
AGTGCCTTGA GGGATGCCCC TGACATCCGG TTCATCTACA CCCCTGCCAT GGAGAGCGTC 
TGCGGATACT TCCACAGGTC CCAGAACCGC AGTGAGGAGT TTCTCATAGC TGGACAATTG 
TCGAATGGGC ACCTGCACAT CACCACCTGC AGTTTTGTGG CTCCCTGGAA CAGCATGAGT 
TCTGCTCAGC GCCGGGGATT CACCAAGACC TATGCTGCTG GTTGTGAGGA ATGCACAGTG 
TTTCCCTGCT CATCCATCCC CTGCAAACTG CAGAGCGACA CTCACTGCTT GTGGACGGAC 
CAGCTGCTCA CAGGCTCTGA CAAGGGGTTC CAGAGCCGCC ACCTGGCCTG CCTGCCCCGG 
GAGCCAGGGC TGTGCACCTG GCAGTCCTTG CGGGCCCAGA TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMP1 ( XM_005228099.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228156.1 Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35539
Clone ID Related Accession (Same CDS sequence)	XM_005228099.1 , XM_005228099.2
Accession Version	XM_005228099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	metalloproteinase inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCAG GAAGCCTGGA GGCCTGTGGT TTCCTACCGC TGCTGCTACC CCGCCCTCGC 
ATGGATATTT ATCCTCCGCA GCTCAGGCCC TGCCGCCATC GCCGCAGACC CAGCGCCCAG 
AGAGGCTACA CCAGAGAACG CACCATGGCC CCCTTTGCAC CCATGGCCTC TGGCATCCTG 
TTGCTGCTGT GGCTCACAGC CCCCAGCCGA GCCTGTACCT GCGTCCCACC CCACCCACAG 
ACGGCCTTCT GCAACTCCGA TGTCGTCATC AGGGCCAAGT TCGTGGGGAC CGCAGAAGTC 
AATGAAACTG CCTTATACCA GCGTTATGAG ATCAAGATGA CTAAGATGTT CAAAGGGTTC 
AGTGCCTTGA GGGATGCCCC TGACATCCGG TTCATCTACA CCCCTGCCAT GGAGAGCGTC 
TGCGGATACT TCCACAGGTC CCAGAACCGC AGTGAGGAGT TTCTCATAGC TGGACAATTG 
TCGAATGGGC ACCTGCACAT CACCACCTGC AGTTTTGTGG CTCCCTGGAA CAGCATGAGT 
TCTGCTCAGC GCCGGGGATT CACCAAGACC TATGCTGCTG GTTGTGAGGA ATGCACAGTG 
TTTCCCTGCT CATCCATCCC CTGCAAACTG CAGAGCGACA CTCACTGCTT GTGGACGGAC 
CAGCTGCTCA CAGGCTCTGA CAAGGGGTTC CAGAGCCGCC ACCTGGCCTG CCTGCCCCGG 
GAGCCAGGGC TGTGCACCTG GCAGTCCTTG CGGGCCCAGA TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228156.1
CDS	84..851
Translation	MHPGSLEACGFLPLLLPRPRMDIYPPQLRPCRHRRRPSAQRGYTRERTMAPFAPMASGILLLLWLTAPSRACTCVPPHPQTAFCNSDVVIRAKFVGTAEVNETALYQRYEIKMTKMFKGFSALRDAPDIRFIYTPAMESVCGYFHRSQNRSEEFLIAGQLSNGHLHITTCSFVAPWNSMSSAQRRGFTKTYAAGCEECTVFPCSSIPCKLQSDTHCLWTDQLLTGSDKGFQSRHLACLPREPGLCTWQSLRAQMA

Target ORF information:

RefSeq Version	XM_005228099.2
Organism	Bos taurus(cattle)
Definition	Bos taurus TIMP metallopeptidase inhibitor 1 (TIMP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228099.2

ORF Insert Sequence:

ATGCATCCAG GAAGCCTGGA GGCCTGTGGT TTCCTACCGC TGCTGCTACC CCGCCCTCGC 
ATGGATATTT ATCCTCCGCA GCTCAGGCCC TGCCGCCATC GCCGCAGACC CAGCGCCCAG 
AGAGGCTACA CCAGAGAACG CACCATGGCC CCCTTTGCAC CCATGGCCTC TGGCATCCTG 
TTGCTGCTGT GGCTCACAGC CCCCAGCCGA GCCTGTACCT GCGTCCCACC CCACCCACAG 
ACGGCCTTCT GCAACTCCGA TGTCGTCATC AGGGCCAAGT TCGTGGGGAC CGCAGAAGTC 
AATGAAACTG CCTTATACCA GCGTTATGAG ATCAAGATGA CTAAGATGTT CAAAGGGTTC 
AGTGCCTTGA GGGATGCCCC TGACATCCGG TTCATCTACA CCCCTGCCAT GGAGAGCGTC 
TGCGGATACT TCCACAGGTC CCAGAACCGC AGTGAGGAGT TTCTCATAGC TGGACAATTG 
TCGAATGGGC ACCTGCACAT CACCACCTGC AGTTTTGTGG CTCCCTGGAA CAGCATGAGT 
TCTGCTCAGC GCCGGGGATT CACCAAGACC TATGCTGCTG GTTGTGAGGA ATGCACAGTG 
TTTCCCTGCT CATCCATCCC CTGCAAACTG CAGAGCGACA CTCACTGCTT GTGGACGGAC 
CAGCTGCTCA CAGGCTCTGA CAAGGGGTTC CAGAGCCGCC ACCTGGCCTG CCTGCCCCGG 
GAGCCAGGGC TGTGCACCTG GCAGTCCTTG CGGGCCCAGA TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decreased expression of matrix metalloproteinase-9 and increased expression of tissue inhibitors of matrix metalloproteinase-1 in paratuberculosis-infected cattle in the ELISA-negative subclinical stage.
Animal biotechnology22(1)44-9(2011 Jan)
Wang X,Wang H,Aodon-geril,Shu Y,Momotani Y,Nagata R,Mori Y,Momotani E

Epidermal growth factor (EGF) induces motility and upregulates MMP-9 and TIMP-1 in bovine trophoblast cells.
Molecular reproduction and development77(7)622-9(2010 Jul)
Dilly M,Hambruch N,Haeger JD,Pfarrer C

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Inhibitory activity on matrix metalloproteinases and cell-proliferating activity of tissue inhibitor of metalloproteinases-1 (TIMP-1)-contrastive difference between human and bovine TIMP-1s on mouse cell proliferation.
Growth factors (Chur, Switzerland)23(2)135-42(2005 Jun)
Nishimura K,Yamashita K,Kato Y,Iwata T,Iwata K,Nishikawa T,Goto S,Hayakawa T

ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells.
Molecular and cellular endocrinology215(1-2)109-14(2004 Feb)
Reichenstein M,Reich R,LeHoux JG,Hanukoglu I

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TIMP1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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