RPE65 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPE65
Entrez Gene ID	282043
Full Name	RPE65, retinoid isomerohydrolase
General protein information	Preferred Names RPE65, retinoid isomerohydrolase Names retinoid isomerohydrolase all-trans-retinyl-palmitate hydrolase meso-zeaxanthin isomerase retinal pigment epithelium-specific 65 kDa protein retinal pigment epithelium-specific protein (65kD) retinal pigment epithelium-specific protein 65kDa retinol isomerase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010803426.2	XP_010801728.1	retinoid isomerohydrolase isoform X1
NM_174453.2	NP_776878.1	retinoid isomerohydrolase
XM_010803426.3	XP_010801728.1	retinoid isomerohydrolase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rpe65	NP_084263.2
	RPE65	XP_001095946.1
Danio rerio (zebrafish)	rpe65c	NP_001107125.1
Xenopus tropicalis (tropical clawed frog)	rpe65	NP_001120538.1
Pan troglodytes (chimpanzee)	RPE65	XP_001165086.1
Canis lupus familiaris (dog)	RPE65	NP_001003176.1
Gallus gallus (chicken)	RPE65	NP_990215.1
Homo sapiens (human)	RPE65	NP_000320.1
Bos taurus (cattle)	RPE65	NP_776878.1
Rattus norvegicus (Norway rat)	Rpe65	NP_446014.2
Danio rerio (zebrafish)	rpe65b	NP_001082902.2
Danio rerio (zebrafish)	rpe65a	NP_957045.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of RPE65 isomerase in a lipidic matrix reveals roles for phospholipids and iron in catalysis.
Kiser PD, Farquhar ER, Shi W, Sui X, Chance MR, Palczewski K
Proceedings of the National Academy of Sciences of the United States of America109(41)E2747-56(2012 Oct)

Cleavage of the retinal pigment epithelium-specific protein RPE65 under oxidative stress.
Lee H, Chung H, Arnouk H, Lamoke F, Hunt RC, Hrushesky WJ, Wood PA, Lee SH, Jahng WJ
International journal of biological macromolecules47(2)104-8(2010 Aug)

Importance of membrane structural integrity for RPE65 retinoid isomerization activity.
Golczak M, Kiser PD, Lodowski DT, Maeda A, Palczewski K
The Journal of biological chemistry285(13)9667-82(2010 Mar)

Rpe65 isomerase associates with membranes through an electrostatic interaction with acidic phospholipid headgroups.
Yuan Q, Kaylor JJ, Miu A, Bassilian S, Whitelegge JP, Travis GH
The Journal of biological chemistry285(2)988-99(2010 Jan)

Crystal structure of native RPE65, the retinoid isomerase of the visual cycle.
Kiser PD, Golczak M, Lodowski DT, Chance MR, Palczewski K
Proceedings of the National Academy of Sciences of the United States of America106(41)17325-30(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Structural differences between delipidated and nondelipidated RPE65 uncovered key residues involved in substrate uptake and processing.
Title: Structure of RPE65 isomerase in a lipidic matrix reveals roles for phospholipids and iron in catalysis.

oxidative stress during the visual cycle results in cleavage of RPE65
Title: Cleavage of the retinal pigment epithelium-specific protein RPE65 under oxidative stress.

the enzymatic activity of native RPE65 strongly depends on its membrane binding and phospholipid environment
Title: Importance of membrane structural integrity for RPE65 retinoid isomerization activity.

Rpe65 isomerase associates with membranes through an electrostatic interaction with acidic phospholipid headgroups.
Title: Rpe65 isomerase associates with membranes through an electrostatic interaction with acidic phospholipid headgroups.

RPE65 from the cone-dominant chicken RPE possesses significantly higher specific retinol isomerohydrolase activity, when compared with RPE65 from rod-dominant species
Title: RPE65 from cone-dominant chicken is a more efficient isomerohydrolase compared with that from rod-dominant species.

The following RPE65 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPE65 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42409	NM_174453.2 Latest version!	Bos taurus retinoid isomerohydrolase RPE65 (RPE65), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45319	XM_010803426.3 Latest version!	Bos taurus RPE65, retinoid isomerohydrolase (RPE65), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa45319	XM_010803426.2	Bos taurus retinal pigment epithelium-specific protein 65kDa (RPE65), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPE65 ( NM_174453.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776878.1 Bos taurus retinoid isomerohydrolase RPE65 (RPE65), mRNA.

Price & Availability↑

CloneID	OBa42409
Clone ID Related Accession (Same CDS sequence)	NM_174453.2
Accession Version	NM_174453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	retinoid isomerohydrolase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L11356.1. On Jun 3, 2003 this sequence version replaced NM_174453.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L11356.1, X66277.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145419, SAMN03145437 [ECO:0000350] ##Evidence-Data-END##

ATGTCCAGCC AAGTTGAACA TCCAGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTATCCT CACCGCTCAC AGCCCATGTT ACAGGCAGGA TCCCCCTCTG GCTAACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAGGTTGGAT CGGAACCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT ACACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTCC GAGGAGTGGA GGTTACTGAC AATGCCCTTG TTAATATCTA CCCAGTGGGG 
GAAGATTACT ATGCCTGCAC AGAGACCAAC TTCATTACAA AGGTTAATCC TGAGACCTTG 
GAAACAATTA AGCAGGTTGA CCTTTGCAAC TATGTCTCAG TCAATGGAGC CACTGCTCAC 
CCCCACATTG AAAATGATGG GACTGTTTAC AACATTGGTA ATTGCTTTGG GAAAAATTTT 
TCAATTGCCT ACAATATTGT AAAGATCCCA CCACTACAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTC 
CATAGTTTTG GTTTGACTCC CAACTATATT GTTTTTGTGG AGACACCAGT CAAAATTAAT 
CTGTTCAAGT TTCTTTCTTC ATGGAGTCTT TGGGGAGCCA ATTACATGGA TTGTTTTGAA 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGAAA AAAGTATATC 
AATAATAAAT ACAGGACCTC TCCTTTTAAC CTCTTTCATC ACATCAATAC CTATGAAGAC 
CATGAGTTTC TGATTGTGGA TCTCTGTTGC TGGAAAGGAT TTGAATTTGT TTATAATTAT 
TCATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCTCAGCCTG AAGTTAGGAG ATACGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACACTCCC CAACACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACC 
ATCTGGCTGG AACCTGAGGT TCTCTTTTCA GGGCCTCGCC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATGG TGGGAAACCT TACACATATG CATATGGACT TGGCTTGAAT 
CACTTTGTTC CAGACAGGCT CTGTAAGCTG AACGTCAAAA CTAAAGAAAC CTGGGTATGG 
CAAGAGCCTG ATTCATACCC CTCAGAACCT ATCTTTGTTT CTCACCCAGA TGCCTTGGAG 
GAAGATGACG GTGTAGTTCT GAGTGTGGTG GTGAGCCCTG GGGCAGGACA AAAGCCTGCT 
TATCTTCTGA TTCTGAATGC CAAGGACTTG AGTGAAGTTG CCAGGGCTGA AGTGGAGATT 
AACATCCCCG TCACCTTTCA TGGACTGTTC AAAAAATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776878.1
CDS	53..1654
Translation	MSSQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNIYPVGEDYYACTETNFITKVNPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYINNKYRTSPFNLFHHINTYEDHEFLIVDLCCWKGFEFVYNYSYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYGGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	NM_174453.2
Organism	Bos taurus(cattle)
Definition	Bos taurus retinoid isomerohydrolase RPE65 (RPE65), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174453.2

ORF Insert Sequence:

ATGTCCAGCC AAGTTGAACA TCCAGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTATCCT CACCGCTCAC AGCCCATGTT ACAGGCAGGA TCCCCCTCTG GCTAACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAGGTTGGAT CGGAACCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT ACACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTCC GAGGAGTGGA GGTTACTGAC AATGCCCTTG TTAATATCTA CCCAGTGGGG 
GAAGATTACT ATGCCTGCAC AGAGACCAAC TTCATTACAA AGGTTAATCC TGAGACCTTG 
GAAACAATTA AGCAGGTTGA CCTTTGCAAC TATGTCTCAG TCAATGGAGC CACTGCTCAC 
CCCCACATTG AAAATGATGG GACTGTTTAC AACATTGGTA ATTGCTTTGG GAAAAATTTT 
TCAATTGCCT ACAATATTGT AAAGATCCCA CCACTACAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTC 
CATAGTTTTG GTTTGACTCC CAACTATATT GTTTTTGTGG AGACACCAGT CAAAATTAAT 
CTGTTCAAGT TTCTTTCTTC ATGGAGTCTT TGGGGAGCCA ATTACATGGA TTGTTTTGAA 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGAAA AAAGTATATC 
AATAATAAAT ACAGGACCTC TCCTTTTAAC CTCTTTCATC ACATCAATAC CTATGAAGAC 
CATGAGTTTC TGATTGTGGA TCTCTGTTGC TGGAAAGGAT TTGAATTTGT TTATAATTAT 
TCATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCTCAGCCTG AAGTTAGGAG ATACGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACACTCCC CAACACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACC 
ATCTGGCTGG AACCTGAGGT TCTCTTTTCA GGGCCTCGCC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATGG TGGGAAACCT TACACATATG CATATGGACT TGGCTTGAAT 
CACTTTGTTC CAGACAGGCT CTGTAAGCTG AACGTCAAAA CTAAAGAAAC CTGGGTATGG 
CAAGAGCCTG ATTCATACCC CTCAGAACCT ATCTTTGTTT CTCACCCAGA TGCCTTGGAG 
GAAGATGACG GTGTAGTTCT GAGTGTGGTG GTGAGCCCTG GGGCAGGACA AAAGCCTGCT 
TATCTTCTGA TTCTGAATGC CAAGGACTTG AGTGAAGTTG CCAGGGCTGA AGTGGAGATT 
AACATCCCCG TCACCTTTCA TGGACTGTTC AAAAAATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPE65 ( XM_010803426.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801728.1 Bos taurus retinal pigment epithelium-specific protein 65kDa (RPE65), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45319
Clone ID Related Accession (Same CDS sequence)	XM_010803426.2 , XM_010803426.3
Accession Version	XM_010803426.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinoid isomerohydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103871.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCT TGAGATTCCA CATGAACTTC TTCCTCATAT TCCACCATTT CAGAGTTGAA 
CATCCAGCTG GTGGTTACAA GAAACTGTTT GAAACTGTGG AGGAACTATC CTCACCGCTC 
ACAGCCCATG TTACAGGCAG GATCCCCCTC TGGCTAACCG GCAGTCTCCT TCGATGTGGG 
CCAGGACTCT TTGAGGTTGG ATCGGAACCA TTTTACCACC TGTTTGATGG GCAAGCCCTC 
CTACACAAGT TTGACTTTAA AGAAGGACAT GTCACATACC ACAGAAGGTT CATCCGCACT 
GATGCTTACG TACGGGCAAT GACTGAGAAA AGGATCGTCA TAACAGAATT TGGCACCTGT 
GCTTTCCCAG ATCCCTGCAA GAATATATTT TCCAGGTTTT TTTCTTACTT CCGAGGAGTG 
GAGGTTACTG ACAATGCCCT TGTTAATATC TACCCAGTGG GGGAAGATTA CTATGCCTGC 
ACAGAGACCA ACTTCATTAC AAAGGTTAAT CCTGAGACCT TGGAAACAAT TAAGCAGGTT 
GACCTTTGCA ACTATGTCTC AGTCAATGGA GCCACTGCTC ACCCCCACAT TGAAAATGAT 
GGGACTGTTT ACAACATTGG TAATTGCTTT GGGAAAAATT TTTCAATTGC CTACAATATT 
GTAAAGATCC CACCACTACA AGCAGACAAG GAAGATCCAA TAAGCAAGTC AGAGATCGTT 
GTACAATTCC CCTGCAGTGA CCGATTCAAG CCATCTTACG TCCATAGTTT TGGTTTGACT 
CCCAACTATA TTGTTTTTGT GGAGACACCA GTCAAAATTA ATCTGTTCAA GTTTCTTTCT 
TCATGGAGTC TTTGGGGAGC CAATTACATG GATTGTTTTG AATCCAATGA AACCATGGGG 
GTTTGGCTTC ATATTGCTGA CAAAAAAAGA AAAAAGTATA TCAATAATAA ATACAGGACC 
TCTCCTTTTA ACCTCTTTCA TCACATCAAT ACCTATGAAG ACCATGAGTT TCTGATTGTG 
GATCTCTGTT GCTGGAAAGG ATTTGAATTT GTTTATAATT ATTTATATTT AGCCAATTTA 
CGTGAGAACT GGGAAGAGGT GAAAAAAAAT GCCAGAAAGG CTCCTCAGCC TGAAGTTAGG 
AGATACGTAC TTCCTTTGAA TATTGACAAG GCTGACACAG GCAAGAATTT AGTCACACTC 
CCCAACACAA CTGCCACTGC AATTCTGTGC AGTGACGAGA CCATCTGGCT GGAACCTGAG 
GTTCTCTTTT CAGGGCCTCG CCAAGCATTT GAGTTTCCTC AAATCAATTA CCAGAAGTAT 
GGTGGGAAAC CTTACACATA TGCATATGGA CTTGGCTTGA ATCACTTTGT TCCAGACAGG 
CTCTGTAAGC TGAACGTCAA AACTAAAGAA ACCTGGGTAT GGCAAGAGCC TGATTCATAC 
CCCTCAGAAC CTATCTTTGT TTCTCACCCA GATGCCTTGG AGGAAGATGA CGGTGTAGTT 
CTGAGTGTGG TGGTGAGCCC TGGGGCAGGA CAAAAGCCTG CTTATCTTCT GATTCTGAAT 
GCCAAGGACT TGAGTGAAGT TGCCAGGGCT GAAGTGGAGA TTAACATCCC CGTCACCTTT 
CATGGACTGT TCAAAAAATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801728.1
CDS	52..1695
Translation	MDSLRFHMNFFLIFHHFRVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNIYPVGEDYYACTETNFITKVNPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYINNKYRTSPFNLFHHINTYEDHEFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYGGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	XM_010803426.2
Organism	Bos taurus(cattle)
Definition	Bos taurus retinal pigment epithelium-specific protein 65kDa (RPE65), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803426.2

ORF Insert Sequence:

ATGGATTCCT TGAGATTCCA CATGAACTTC TTCCTCATAT TCCACCATTT CAGAGTTGAA 
CATCCAGCTG GTGGTTACAA GAAACTGTTT GAAACTGTGG AGGAACTATC CTCACCGCTC 
ACAGCCCATG TTACAGGCAG GATCCCCCTC TGGCTAACCG GCAGTCTCCT TCGATGTGGG 
CCAGGACTCT TTGAGGTTGG ATCGGAACCA TTTTACCACC TGTTTGATGG GCAAGCCCTC 
CTACACAAGT TTGACTTTAA AGAAGGACAT GTCACATACC ACAGAAGGTT CATCCGCACT 
GATGCTTACG TACGGGCAAT GACTGAGAAA AGGATCGTCA TAACAGAATT TGGCACCTGT 
GCTTTCCCAG ATCCCTGCAA GAATATATTT TCCAGGTTTT TTTCTTACTT CCGAGGAGTG 
GAGGTTACTG ACAATGCCCT TGTTAATATC TACCCAGTGG GGGAAGATTA CTATGCCTGC 
ACAGAGACCA ACTTCATTAC AAAGGTTAAT CCTGAGACCT TGGAAACAAT TAAGCAGGTT 
GACCTTTGCA ACTATGTCTC AGTCAATGGA GCCACTGCTC ACCCCCACAT TGAAAATGAT 
GGGACTGTTT ACAACATTGG TAATTGCTTT GGGAAAAATT TTTCAATTGC CTACAATATT 
GTAAAGATCC CACCACTACA AGCAGACAAG GAAGATCCAA TAAGCAAGTC AGAGATCGTT 
GTACAATTCC CCTGCAGTGA CCGATTCAAG CCATCTTACG TCCATAGTTT TGGTTTGACT 
CCCAACTATA TTGTTTTTGT GGAGACACCA GTCAAAATTA ATCTGTTCAA GTTTCTTTCT 
TCATGGAGTC TTTGGGGAGC CAATTACATG GATTGTTTTG AATCCAATGA AACCATGGGG 
GTTTGGCTTC ATATTGCTGA CAAAAAAAGA AAAAAGTATA TCAATAATAA ATACAGGACC 
TCTCCTTTTA ACCTCTTTCA TCACATCAAT ACCTATGAAG ACCATGAGTT TCTGATTGTG 
GATCTCTGTT GCTGGAAAGG ATTTGAATTT GTTTATAATT ATTTATATTT AGCCAATTTA 
CGTGAGAACT GGGAAGAGGT GAAAAAAAAT GCCAGAAAGG CTCCTCAGCC TGAAGTTAGG 
AGATACGTAC TTCCTTTGAA TATTGACAAG GCTGACACAG GCAAGAATTT AGTCACACTC 
CCCAACACAA CTGCCACTGC AATTCTGTGC AGTGACGAGA CCATCTGGCT GGAACCTGAG 
GTTCTCTTTT CAGGGCCTCG CCAAGCATTT GAGTTTCCTC AAATCAATTA CCAGAAGTAT 
GGTGGGAAAC CTTACACATA TGCATATGGA CTTGGCTTGA ATCACTTTGT TCCAGACAGG 
CTCTGTAAGC TGAACGTCAA AACTAAAGAA ACCTGGGTAT GGCAAGAGCC TGATTCATAC 
CCCTCAGAAC CTATCTTTGT TTCTCACCCA GATGCCTTGG AGGAAGATGA CGGTGTAGTT 
CTGAGTGTGG TGGTGAGCCC TGGGGCAGGA CAAAAGCCTG CTTATCTTCT GATTCTGAAT 
GCCAAGGACT TGAGTGAAGT TGCCAGGGCT GAAGTGGAGA TTAACATCCC CGTCACCTTT 
CATGGACTGT TCAAAAAATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPE65 ( XM_010803426.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801728.1 Bos taurus RPE65, retinoid isomerohydrolase (RPE65), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45319
Clone ID Related Accession (Same CDS sequence)	XM_010803426.2 , XM_010803426.3
Accession Version	XM_010803426.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	retinoid isomerohydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCT TGAGATTCCA CATGAACTTC TTCCTCATAT TCCACCATTT CAGAGTTGAA 
CATCCAGCTG GTGGTTACAA GAAACTGTTT GAAACTGTGG AGGAACTATC CTCACCGCTC 
ACAGCCCATG TTACAGGCAG GATCCCCCTC TGGCTAACCG GCAGTCTCCT TCGATGTGGG 
CCAGGACTCT TTGAGGTTGG ATCGGAACCA TTTTACCACC TGTTTGATGG GCAAGCCCTC 
CTACACAAGT TTGACTTTAA AGAAGGACAT GTCACATACC ACAGAAGGTT CATCCGCACT 
GATGCTTACG TACGGGCAAT GACTGAGAAA AGGATCGTCA TAACAGAATT TGGCACCTGT 
GCTTTCCCAG ATCCCTGCAA GAATATATTT TCCAGGTTTT TTTCTTACTT CCGAGGAGTG 
GAGGTTACTG ACAATGCCCT TGTTAATATC TACCCAGTGG GGGAAGATTA CTATGCCTGC 
ACAGAGACCA ACTTCATTAC AAAGGTTAAT CCTGAGACCT TGGAAACAAT TAAGCAGGTT 
GACCTTTGCA ACTATGTCTC AGTCAATGGA GCCACTGCTC ACCCCCACAT TGAAAATGAT 
GGGACTGTTT ACAACATTGG TAATTGCTTT GGGAAAAATT TTTCAATTGC CTACAATATT 
GTAAAGATCC CACCACTACA AGCAGACAAG GAAGATCCAA TAAGCAAGTC AGAGATCGTT 
GTACAATTCC CCTGCAGTGA CCGATTCAAG CCATCTTACG TCCATAGTTT TGGTTTGACT 
CCCAACTATA TTGTTTTTGT GGAGACACCA GTCAAAATTA ATCTGTTCAA GTTTCTTTCT 
TCATGGAGTC TTTGGGGAGC CAATTACATG GATTGTTTTG AATCCAATGA AACCATGGGG 
GTTTGGCTTC ATATTGCTGA CAAAAAAAGA AAAAAGTATA TCAATAATAA ATACAGGACC 
TCTCCTTTTA ACCTCTTTCA TCACATCAAT ACCTATGAAG ACCATGAGTT TCTGATTGTG 
GATCTCTGTT GCTGGAAAGG ATTTGAATTT GTTTATAATT ATTTATATTT AGCCAATTTA 
CGTGAGAACT GGGAAGAGGT GAAAAAAAAT GCCAGAAAGG CTCCTCAGCC TGAAGTTAGG 
AGATACGTAC TTCCTTTGAA TATTGACAAG GCTGACACAG GCAAGAATTT AGTCACACTC 
CCCAACACAA CTGCCACTGC AATTCTGTGC AGTGACGAGA CCATCTGGCT GGAACCTGAG 
GTTCTCTTTT CAGGGCCTCG CCAAGCATTT GAGTTTCCTC AAATCAATTA CCAGAAGTAT 
GGTGGGAAAC CTTACACATA TGCATATGGA CTTGGCTTGA ATCACTTTGT TCCAGACAGG 
CTCTGTAAGC TGAACGTCAA AACTAAAGAA ACCTGGGTAT GGCAAGAGCC TGATTCATAC 
CCCTCAGAAC CTATCTTTGT TTCTCACCCA GATGCCTTGG AGGAAGATGA CGGTGTAGTT 
CTGAGTGTGG TGGTGAGCCC TGGGGCAGGA CAAAAGCCTG CTTATCTTCT GATTCTGAAT 
GCCAAGGACT TGAGTGAAGT TGCCAGGGCT GAAGTGGAGA TTAACATCCC CGTCACCTTT 
CATGGACTGT TCAAAAAATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801728.1
CDS	39..1682
Translation	MDSLRFHMNFFLIFHHFRVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNIYPVGEDYYACTETNFITKVNPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYINNKYRTSPFNLFHHINTYEDHEFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYGGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	XM_010803426.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RPE65, retinoid isomerohydrolase (RPE65), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803426.3

ORF Insert Sequence:

ATGGATTCCT TGAGATTCCA CATGAACTTC TTCCTCATAT TCCACCATTT CAGAGTTGAA 
CATCCAGCTG GTGGTTACAA GAAACTGTTT GAAACTGTGG AGGAACTATC CTCACCGCTC 
ACAGCCCATG TTACAGGCAG GATCCCCCTC TGGCTAACCG GCAGTCTCCT TCGATGTGGG 
CCAGGACTCT TTGAGGTTGG ATCGGAACCA TTTTACCACC TGTTTGATGG GCAAGCCCTC 
CTACACAAGT TTGACTTTAA AGAAGGACAT GTCACATACC ACAGAAGGTT CATCCGCACT 
GATGCTTACG TACGGGCAAT GACTGAGAAA AGGATCGTCA TAACAGAATT TGGCACCTGT 
GCTTTCCCAG ATCCCTGCAA GAATATATTT TCCAGGTTTT TTTCTTACTT CCGAGGAGTG 
GAGGTTACTG ACAATGCCCT TGTTAATATC TACCCAGTGG GGGAAGATTA CTATGCCTGC 
ACAGAGACCA ACTTCATTAC AAAGGTTAAT CCTGAGACCT TGGAAACAAT TAAGCAGGTT 
GACCTTTGCA ACTATGTCTC AGTCAATGGA GCCACTGCTC ACCCCCACAT TGAAAATGAT 
GGGACTGTTT ACAACATTGG TAATTGCTTT GGGAAAAATT TTTCAATTGC CTACAATATT 
GTAAAGATCC CACCACTACA AGCAGACAAG GAAGATCCAA TAAGCAAGTC AGAGATCGTT 
GTACAATTCC CCTGCAGTGA CCGATTCAAG CCATCTTACG TCCATAGTTT TGGTTTGACT 
CCCAACTATA TTGTTTTTGT GGAGACACCA GTCAAAATTA ATCTGTTCAA GTTTCTTTCT 
TCATGGAGTC TTTGGGGAGC CAATTACATG GATTGTTTTG AATCCAATGA AACCATGGGG 
GTTTGGCTTC ATATTGCTGA CAAAAAAAGA AAAAAGTATA TCAATAATAA ATACAGGACC 
TCTCCTTTTA ACCTCTTTCA TCACATCAAT ACCTATGAAG ACCATGAGTT TCTGATTGTG 
GATCTCTGTT GCTGGAAAGG ATTTGAATTT GTTTATAATT ATTTATATTT AGCCAATTTA 
CGTGAGAACT GGGAAGAGGT GAAAAAAAAT GCCAGAAAGG CTCCTCAGCC TGAAGTTAGG 
AGATACGTAC TTCCTTTGAA TATTGACAAG GCTGACACAG GCAAGAATTT AGTCACACTC 
CCCAACACAA CTGCCACTGC AATTCTGTGC AGTGACGAGA CCATCTGGCT GGAACCTGAG 
GTTCTCTTTT CAGGGCCTCG CCAAGCATTT GAGTTTCCTC AAATCAATTA CCAGAAGTAT 
GGTGGGAAAC CTTACACATA TGCATATGGA CTTGGCTTGA ATCACTTTGT TCCAGACAGG 
CTCTGTAAGC TGAACGTCAA AACTAAAGAA ACCTGGGTAT GGCAAGAGCC TGATTCATAC 
CCCTCAGAAC CTATCTTTGT TTCTCACCCA GATGCCTTGG AGGAAGATGA CGGTGTAGTT 
CTGAGTGTGG TGGTGAGCCC TGGGGCAGGA CAAAAGCCTG CTTATCTTCT GATTCTGAAT 
GCCAAGGACT TGAGTGAAGT TGCCAGGGCT GAAGTGGAGA TTAACATCCC CGTCACCTTT 
CATGGACTGT TCAAAAAATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of RPE65 isomerase in a lipidic matrix reveals roles for phospholipids and iron in catalysis.
Proceedings of the National Academy of Sciences of the United States of America109(41)E2747-56(2012 Oct)
Kiser PD,Farquhar ER,Shi W,Sui X,Chance MR,Palczewski K

Cleavage of the retinal pigment epithelium-specific protein RPE65 under oxidative stress.
International journal of biological macromolecules47(2)104-8(2010 Aug)
Lee H,Chung H,Arnouk H,Lamoke F,Hunt RC,Hrushesky WJ,Wood PA,Lee SH,Jahng WJ

Importance of membrane structural integrity for RPE65 retinoid isomerization activity.
The Journal of biological chemistry285(13)9667-82(2010 Mar)
Golczak M,Kiser PD,Lodowski DT,Maeda A,Palczewski K

Rpe65 isomerase associates with membranes through an electrostatic interaction with acidic phospholipid headgroups.
The Journal of biological chemistry285(2)988-99(2010 Jan)
Yuan Q,Kaylor JJ,Miu A,Bassilian S,Whitelegge JP,Travis GH

Crystal structure of native RPE65, the retinoid isomerase of the visual cycle.
Proceedings of the National Academy of Sciences of the United States of America106(41)17325-30(2009 Oct)
Kiser PD,Golczak M,Lodowski DT,Chance MR,Palczewski K

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

RPE65 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報