PTGS2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTGS2
Entrez Gene ID	282023
Full Name	prostaglandin-endoperoxide synthase 2
General protein information	Preferred Names prostaglandin-endoperoxide synthase 2 Names prostaglandin G/H synthase 2 COX-2 PGH synthase 2 PGHS-2 PHS II cyclooxygenase-2 prostaglandin H2 synthase 2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010813539.2	XP_010811841.1	prostaglandin G/H synthase 2 isoform X1
XM_015475265.1	XP_015330751.1	prostaglandin G/H synthase 2 isoform X2
XM_010813540.2	XP_010811842.1	prostaglandin G/H synthase 2 isoform X3
NM_174445.2	NP_776870.1	prostaglandin G/H synthase 2 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PTGS2	NP_001161191.1
Danio rerio (zebrafish)	ptgs2b	NP_001020675.1
Mus musculus (house mouse)	Ptgs2	NP_035328.2
Canis lupus familiaris (dog)	PTGS2	NP_001003354.1
Pan troglodytes (chimpanzee)	PTGS2	XP_524999.3
	PTGS2	XP_001107538.2
Rattus norvegicus (Norway rat)	Ptgs2	NP_058928.3
Homo sapiens (human)	PTGS2	NP_000954.1
Bos taurus (cattle)	PTGS2	NP_776870.1
Xenopus tropicalis (tropical clawed frog)	ptgs2	NP_001025697.1

Gene Ontology
Bibliography

Related articles in PubMed

PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle.
Gao L, Liu B, Mao W, Gao R, Zhang S, Duritahala, Fu C, Shen Y, Zhang Y, Zhang N, Wu J, Deng Y, Wu X, Cao J
Animal reproduction science18754-63(2017 Dec)

Endometrial mRNA expression of prostaglandin synthase enzymes PTGS 2, PTGFS and mPTGES 1 in repeat-breeding cows with cytologically determined endometritis.
Janowski T, Bara?ski W, ?ukasik K, Skar?y?ski D, Zdu?czyk S, Malinowska K
Acta veterinaria Hungarica65(1)96-104(2017 03)

Decreased cyclooxygenase-2 gene expression and lactoferrin release in blood neutrophils of heifers during the calving period.
Hidalgo MA, Loncom?n CA, Hidalgo AI, Andrade V, Carretta MD, Burgos RA
Veterinary immunology and immunopathology160(1-2)139-44(2014 Jul)

REV-ERB? inhibits the PTGS2 expression in bovine uterus endometrium stromal and epithelial cells exposed to ovarian steroids.
Isayama K, Chen H, Yamauchi N, Hattori MA
The Journal of reproduction and development60(5)362-70(2014)

Altered expression of inflammation-associated genes in oviductal cells following follicular fluid exposure: implications for ovarian carcinogenesis.
Lau A, Kollara A, St John E, Tone AA, Virtanen C, Greenblatt EM, King WA, Brown TJ
Experimental biology and medicine (Maywood, N.J.)239(1)24-32(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The objective of this study was to evaluate the mRNA expression of prostaglandin-endoperoxide synthase 2 (PTGS 2), prostaglandin F2alpha synthase (PTGFS) and prostaglandin E2 microsomal synthase 1 (mPTGES 1) in the endometrium of repeat-breeding cows with and without subclinical endometritis.
Title: Endometrial mRNA expression of prostaglandin synthase enzymes PTGS 2, PTGFS and mPTGES 1 in repeat-breeding cows with cytologically determined endometritis.

This study showed that neutrophils from periparturient heifers show impairment of COX-2 mRNA expression and lactoferrin, suggesting that these mechanisms may contribute to immunosuppression in cows around calving.
Title: Decreased cyclooxygenase-2 gene expression and lactoferrin release in blood neutrophils of heifers during the calving period.

results indicate that nuclear receptor subfamily 1 group D member 1(REV-ERBalpha) plays an inhibitory role in the expression of prostaglandin-endoperoxide synthase 2(PTGS2) in both bovine USCs and UECs treated with ovarian steroids
Title: REV-ERB? inhibits the PTGS2 expression in bovine uterus endometrium stromal and epithelial cells exposed to ovarian steroids.

Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected.
Title: Altered expression of inflammation-associated genes in oviductal cells following follicular fluid exposure: implications for ovarian carcinogenesis.

Purinergic P2Y1 receptor signaling mediates wound stimuli-induced cyclooxygenase-2 expression in intestinal subepithelial myofibroblasts
Title: Purinergic P2Y1 receptor signaling mediates wound stimuli-induced cyclooxygenase-2 expression in intestinal subepithelial myofibroblasts.

The following PTGS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTGS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01354	NM_174445.2 Latest version!	Bos taurus prostaglandin-endoperoxide synthase 2 (PTGS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45312	XM_010813539.2 Latest version!	Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45313	XM_010813540.2 Latest version!	Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa93053	XM_015475265.1 Latest version!	Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTGS2 ( NM_174445.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776870.1 Bos taurus prostaglandin-endoperoxide synthase 2 (PTGS2), mRNA.

Price & Availability↑

CloneID	OBa01354
Clone ID Related Accession (Same CDS sequence)	NM_174445.2
Accession Version	NM_174445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	prostaglandin G/H synthase 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF031698.1. On Jun 3, 2003 this sequence version replaced NM_174445.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF031698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTCGCCC GGGCCCTGCT GCTCTGCGCT GCCGTGGCGC TCAGCGGTGC AGCAAATCCT 
TGCTGTTCCC ATCCATGTCA GAATCGAGGT GTATGTATGA GTGTAGGATT TGACCAGTAT 
AAATGTGACT GTACCCGAAC AGGATTCTAC GGTGAAAACT GTACCACACC CGAATTTCTG 
ACAAGAATAA AATTACTCCT GAAACCCACT CCCAACACAG TGCACTACAT ACTTACCCAC 
TTCAAAGGAG TCTGGAACAT TGTCAATAAG ATCTCCTTCC TGCGAAATAT GATTATGAGA 
TATGTGTTGA CGTCGAGATC ACATTTGATT GAGAGTCCAC CAACTTATAA TGTGCACTAC 
AGCTATAAAA GCTGGGAAGC CTTTTCTAAC CTGTCTTATT ATACCAGAGC TCTTCCTCCT 
GTGCCTGATG ACTGCCCAAC ACCCATGGGT GTGAAAGGGA GGAAAGAGCT TCCTGATTCA 
AAAGAAGTTG TGAAAAAAGT ACTTCTAAGA AGAAAGTTCA TTCCTGATCC CCAGGGCACA 
AATCTGATGT TTGCATTCTT TGCCCAGCAC TTCACCCATC AATTTTTCAA GACAGATTTT 
GAACGAGGAC CAGCTTTCAC TAAGGGAAAG AACCATGGGG TGGACTTAAG TCACATTTAT 
GGTGAATCTT TAGAGAGACA GCATAAGCTG CGCCTTTTCA AGGATGGAAA AATGAAATAT 
CAGATGATTA ATGGAGAGAT GTATCCTCCC ACAGTCAAAG ATACTCAGGT CGAAATGATC 
TACCCGCCTC ATGTTCCTGA ACACTTGAAG TTTGCTGTGG GCCAGGAAGT CTTTGGTCTG 
GTGCCTGGTC TGATGATGTA TGCCACCATT TGGCTACGGG AACACAACAG AGTGTGTGAT 
GTGCTTAAAC AAGAGCATCC AGAATGGGGC GATGAGCAGT TGTTCCAGAC AAGCAGGCTA 
ATCCTGATAG GAGAAACTAT TAAGATTGTG ATTGAAGACT ACGTACAGCA CTTGAGTGGC 
TATCACTTCA AACTGAAGTT TGACCCAGAG CTGCTTTTCA ACCAACAGTT CCAGTACCAG 
AACCGTATTG CTGCTGAGTT TAACACGCTC TACCACTGGC ATCCCCTTCT GCCTGACGTC 
TTTCAGATTG ATGGTCAGGA GTACAACTAT CAGCAGTTTA TCTATAACAA CTCTGTCTTA 
CTGGAACATG GTCTCACTCA GTTTGTTGAA TCATTCACCA GGCAAAGGGC TGGCAGGGTC 
GCTGGCGGTA GGAATCTTCC AGTCGCAGTA GAGAAAGTAT CAAAGGCTTC AATTGACCAG 
AGCAGAGAGA TGAAATACCA GTCTTTTAAT GAGTATCGCA AACGTTTTCT CGTGAAGCCC 
TATGAATCAT TTGAGGAACT TACAGGAGAG AAGGAAATGG CTGCAGAGTT AGAAGCGCTC 
TATGGAGACA TAGATGCCAT GGAGTTTTAT CCCGCCCTTC TGGTAGAGAA GCCCCGTCCA 
GACGCCATCT TTGGGGAGAC CATGGTAGAA GCTGGAGCAC CATTCTCCCT GAAAGGACTT 
ATGGGTAATC CTATATGCTC TCCCGAGTAC TGGAAGCCTA GCACTTTCGG TGGAGAAGTA 
GGTTTTAAAA TCATCAACAC TGCCTCAATT CAGTCTCTCA TCTGCAGTAA CGTGAAAGGC 
TGTCCCTTTA CCTCATTCAG TGTTCAAGAT ACGCACCTCA CCAAAACGGT CACCATTAAT 
GCAAGCTCTT CCCACTCTGG ACTAGATGAT ATCAACCCCA CAGTCCTACT AAAGGAACGT 
TCAACCGAAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776870.1
CDS	110..1924
Translation	MLARALLLCAAVALSGAANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCTTPEFLTRIKLLLKPTPNTVHYILTHFKGVWNIVNKISFLRNMIMRYVLTSRSHLIESPPTYNVHYSYKSWEAFSNLSYYTRALPPVPDDCPTPMGVKGRKELPDSKEVVKKVLLRRKFIPDPQGTNLMFAFFAQHFTHQFFKTDFERGPAFTKGKNHGVDLSHIYGESLERQHKLRLFKDGKMKYQMINGEMYPPTVKDTQVEMIYPPHVPEHLKFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNQQFQYQNRIAAEFNTLYHWHPLLPDVFQIDGQEYNYQQFIYNNSVLLEHGLTQFVESFTRQRAGRVAGGRNLPVAVEKVSKASIDQSREMKYQSFNEYRKRFLVKPYESFEELTGEKEMAAELEALYGDIDAMEFYPALLVEKPRPDAIFGETMVEAGAPFSLKGLMGNPICSPEYWKPSTFGGEVGFKIINTASIQSLICSNVKGCPFTSFSVQDTHLTKTVTINASSSHSGLDDINPTVLLKERSTEL

Target ORF information:

RefSeq Version	NM_174445.2
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin-endoperoxide synthase 2 (PTGS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174445.2

ORF Insert Sequence:

ATGCTCGCCC GGGCCCTGCT GCTCTGCGCT GCCGTGGCGC TCAGCGGTGC AGCAAATCCT 
TGCTGTTCCC ATCCATGTCA GAATCGAGGT GTATGTATGA GTGTAGGATT TGACCAGTAT 
AAATGTGACT GTACCCGAAC AGGATTCTAC GGTGAAAACT GTACCACACC CGAATTTCTG 
ACAAGAATAA AATTACTCCT GAAACCCACT CCCAACACAG TGCACTACAT ACTTACCCAC 
TTCAAAGGAG TCTGGAACAT TGTCAATAAG ATCTCCTTCC TGCGAAATAT GATTATGAGA 
TATGTGTTGA CGTCGAGATC ACATTTGATT GAGAGTCCAC CAACTTATAA TGTGCACTAC 
AGCTATAAAA GCTGGGAAGC CTTTTCTAAC CTGTCTTATT ATACCAGAGC TCTTCCTCCT 
GTGCCTGATG ACTGCCCAAC ACCCATGGGT GTGAAAGGGA GGAAAGAGCT TCCTGATTCA 
AAAGAAGTTG TGAAAAAAGT ACTTCTAAGA AGAAAGTTCA TTCCTGATCC CCAGGGCACA 
AATCTGATGT TTGCATTCTT TGCCCAGCAC TTCACCCATC AATTTTTCAA GACAGATTTT 
GAACGAGGAC CAGCTTTCAC TAAGGGAAAG AACCATGGGG TGGACTTAAG TCACATTTAT 
GGTGAATCTT TAGAGAGACA GCATAAGCTG CGCCTTTTCA AGGATGGAAA AATGAAATAT 
CAGATGATTA ATGGAGAGAT GTATCCTCCC ACAGTCAAAG ATACTCAGGT CGAAATGATC 
TACCCGCCTC ATGTTCCTGA ACACTTGAAG TTTGCTGTGG GCCAGGAAGT CTTTGGTCTG 
GTGCCTGGTC TGATGATGTA TGCCACCATT TGGCTACGGG AACACAACAG AGTGTGTGAT 
GTGCTTAAAC AAGAGCATCC AGAATGGGGC GATGAGCAGT TGTTCCAGAC AAGCAGGCTA 
ATCCTGATAG GAGAAACTAT TAAGATTGTG ATTGAAGACT ACGTACAGCA CTTGAGTGGC 
TATCACTTCA AACTGAAGTT TGACCCAGAG CTGCTTTTCA ACCAACAGTT CCAGTACCAG 
AACCGTATTG CTGCTGAGTT TAACACGCTC TACCACTGGC ATCCCCTTCT GCCTGACGTC 
TTTCAGATTG ATGGTCAGGA GTACAACTAT CAGCAGTTTA TCTATAACAA CTCTGTCTTA 
CTGGAACATG GTCTCACTCA GTTTGTTGAA TCATTCACCA GGCAAAGGGC TGGCAGGGTC 
GCTGGCGGTA GGAATCTTCC AGTCGCAGTA GAGAAAGTAT CAAAGGCTTC AATTGACCAG 
AGCAGAGAGA TGAAATACCA GTCTTTTAAT GAGTATCGCA AACGTTTTCT CGTGAAGCCC 
TATGAATCAT TTGAGGAACT TACAGGAGAG AAGGAAATGG CTGCAGAGTT AGAAGCGCTC 
TATGGAGACA TAGATGCCAT GGAGTTTTAT CCCGCCCTTC TGGTAGAGAA GCCCCGTCCA 
GACGCCATCT TTGGGGAGAC CATGGTAGAA GCTGGAGCAC CATTCTCCCT GAAAGGACTT 
ATGGGTAATC CTATATGCTC TCCCGAGTAC TGGAAGCCTA GCACTTTCGG TGGAGAAGTA 
GGTTTTAAAA TCATCAACAC TGCCTCAATT CAGTCTCTCA TCTGCAGTAA CGTGAAAGGC 
TGTCCCTTTA CCTCATTCAG TGTTCAAGAT ACGCACCTCA CCAAAACGGT CACCATTAAT 
GCAAGCTCTT CCCACTCTGG ACTAGATGAT ATCAACCCCA CAGTCCTACT AAAGGAACGT 
TCAACCGAAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGS2 ( XM_010813539.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811841.1 Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45312
Clone ID Related Accession (Same CDS sequence)	XM_010813539.2
Accession Version	XM_010813539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prostaglandin G/H synthase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCCC GGGCCCTGCT GCTCTGCGCT GCCGTGGCGC TCAGCGGTGC AGCAAATCCT 
TGCTGTTCCC ATCCATGCCA GAATCGAGGT GTATGTATGA GTGTAGGATT TGACCAGTAT 
AAATGTGACT GTACCCGAAC AGGATTCTAC GGTGAAAACT GTACCACACC CGAATTTCTG 
ACAAGAATAA AATTACTCCT GAAACCCACT CCCAACACAG TGCACTACAT ACTTACCCAC 
TTCAAAGGAG TCTGGAACAT TGTCAATAAG ATCTCCTTCC TGCGAAATAT GATTATGAGA 
TATGTGTTGA CGTCGAGATC ACATTTGATT GAGAGTCCAC CAACTTATAA TGTGCACTAC 
AGCTATAAAA GCTGGGAAGC CTTTTCTAAC CTGTCTTATT ATACCAGAGC TCTTCCTCCT 
GTGCCTGATG ACTGCCCAAC ACCCATGGGT GTGAAAGGGA GGAAAGAGCT TCCTGATTCA 
AAAGAAGTTG TGAAAAAAGT ACTTCTAAGA AGAAAGTTCA TTCCTGATCC CCAGGGCACA 
AATCTGATGT TTGCATTCTT TGCCCAGCAC TTCACCCATC AATTTTTCAA GACAGATTTT 
GAACGAGGAC CAGCTTTCAC TAAGGGAAAG AACCATGGGG TGGACTTAAG TCACATTTAT 
GGTGAATCTT TAGAGAGACA GCATAAGCTG CGCCTTTTCA AGGATGGAAA AATGAAATAT 
CAGATGATTA ATGGAGAGAT GTATCCTCCC ACAGTCAAAG ATACTCAGGT CGAAATGATC 
TACCCGCCTC ATGTTCCTGA ACACTTGAAG TTTGCTGTGG GCCAGGAAGT CTTTGGTCTG 
GTGCCTGGTC TGATGATGTA TGCCACCATT TGGCTACGGG AACACAACAG AGTGTGTGAT 
GTGCTTAAAC AAGAGCATCC AGAATGGGGC GATGAGCAGT TGTTCCAGAC AAGCAGGCTA 
ATCCTGATAG GAGAAACTAT TAAGATTGTG ATTGAAGACT ACGTACAGCA CTTGAGTGGC 
TATCACTTCA AACTGAAGTT TGACCCAGAG CTGCTTTTCA ACCAACAGTT CCAGTACCAG 
AACCGTATTG CTGCTGAGTT TAACACGCTC TACCACTGGC ATCCCCTTCT GCCTGACGTC 
TTTCAGATTG ATGGTCAGGA GTACAACTAT CAGCAGTTTA TCTATAACAA CTCTGTCTTA 
CTGGAACATG GTCTCACTCA GTTTGTTGAA TCATTCACCA GGCAAAGGGC TGGCAGGGTC 
GCTGGCGGTA GGAATCTTCC AGTCGCAGTA GAGAAAGTAT CAAAGGCTTC AATTGACCAG 
AGCAGAGAGA TGAAATACCA GTCTTTTAAT GAGTATCGCA AACGTTTTCT GCTGAAGCCC 
TATGAATCAT TTGAGGAACT TACAGGAGAG AAGGAAATGG CTGCAGAGTT AGAAGCGCTC 
TATGGAGACA TAGATGCCAT GGAGTTTTAT CCCGCCCTTC TGGTAGAGAA GCCCCGTCCA 
GACGCCATCT TTGGGGAGAC CATGGTAGAA GCTGGAGCAC CATTCTCCCT GAAAGGACTT 
ATGGGTAATC CTATATGCTC TCCCGAGTAC TGGAAGCCTA GCACTTTCGG TGGAGAAGTA 
GGTTTTAAAA TCATCAACAC TGCCTCAATT CAGTCTCTCA TCTGCAGTAA CGTGAAAGGC 
TGTCCCTTTA CCTCATTCAG TGTTCAAGAT ACGCACCTCA CCAAAACGGT CACCATTAAT 
GCAAGCTCTT CCCACTCTGG ACTAGATGAT ATCAACCCCA CAGTCCTACT AAAGGAACGT 
TCAACCGAAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811841.1
CDS	292..2106
Translation	MLARALLLCAAVALSGAANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCTTPEFLTRIKLLLKPTPNTVHYILTHFKGVWNIVNKISFLRNMIMRYVLTSRSHLIESPPTYNVHYSYKSWEAFSNLSYYTRALPPVPDDCPTPMGVKGRKELPDSKEVVKKVLLRRKFIPDPQGTNLMFAFFAQHFTHQFFKTDFERGPAFTKGKNHGVDLSHIYGESLERQHKLRLFKDGKMKYQMINGEMYPPTVKDTQVEMIYPPHVPEHLKFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNQQFQYQNRIAAEFNTLYHWHPLLPDVFQIDGQEYNYQQFIYNNSVLLEHGLTQFVESFTRQRAGRVAGGRNLPVAVEKVSKASIDQSREMKYQSFNEYRKRFLLKPYESFEELTGEKEMAAELEALYGDIDAMEFYPALLVEKPRPDAIFGETMVEAGAPFSLKGLMGNPICSPEYWKPSTFGGEVGFKIINTASIQSLICSNVKGCPFTSFSVQDTHLTKTVTINASSSHSGLDDINPTVLLKERSTEL

Target ORF information:

RefSeq Version	XM_010813539.2
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813539.2

ORF Insert Sequence:

ATGCTCGCCC GGGCCCTGCT GCTCTGCGCT GCCGTGGCGC TCAGCGGTGC AGCAAATCCT 
TGCTGTTCCC ATCCATGCCA GAATCGAGGT GTATGTATGA GTGTAGGATT TGACCAGTAT 
AAATGTGACT GTACCCGAAC AGGATTCTAC GGTGAAAACT GTACCACACC CGAATTTCTG 
ACAAGAATAA AATTACTCCT GAAACCCACT CCCAACACAG TGCACTACAT ACTTACCCAC 
TTCAAAGGAG TCTGGAACAT TGTCAATAAG ATCTCCTTCC TGCGAAATAT GATTATGAGA 
TATGTGTTGA CGTCGAGATC ACATTTGATT GAGAGTCCAC CAACTTATAA TGTGCACTAC 
AGCTATAAAA GCTGGGAAGC CTTTTCTAAC CTGTCTTATT ATACCAGAGC TCTTCCTCCT 
GTGCCTGATG ACTGCCCAAC ACCCATGGGT GTGAAAGGGA GGAAAGAGCT TCCTGATTCA 
AAAGAAGTTG TGAAAAAAGT ACTTCTAAGA AGAAAGTTCA TTCCTGATCC CCAGGGCACA 
AATCTGATGT TTGCATTCTT TGCCCAGCAC TTCACCCATC AATTTTTCAA GACAGATTTT 
GAACGAGGAC CAGCTTTCAC TAAGGGAAAG AACCATGGGG TGGACTTAAG TCACATTTAT 
GGTGAATCTT TAGAGAGACA GCATAAGCTG CGCCTTTTCA AGGATGGAAA AATGAAATAT 
CAGATGATTA ATGGAGAGAT GTATCCTCCC ACAGTCAAAG ATACTCAGGT CGAAATGATC 
TACCCGCCTC ATGTTCCTGA ACACTTGAAG TTTGCTGTGG GCCAGGAAGT CTTTGGTCTG 
GTGCCTGGTC TGATGATGTA TGCCACCATT TGGCTACGGG AACACAACAG AGTGTGTGAT 
GTGCTTAAAC AAGAGCATCC AGAATGGGGC GATGAGCAGT TGTTCCAGAC AAGCAGGCTA 
ATCCTGATAG GAGAAACTAT TAAGATTGTG ATTGAAGACT ACGTACAGCA CTTGAGTGGC 
TATCACTTCA AACTGAAGTT TGACCCAGAG CTGCTTTTCA ACCAACAGTT CCAGTACCAG 
AACCGTATTG CTGCTGAGTT TAACACGCTC TACCACTGGC ATCCCCTTCT GCCTGACGTC 
TTTCAGATTG ATGGTCAGGA GTACAACTAT CAGCAGTTTA TCTATAACAA CTCTGTCTTA 
CTGGAACATG GTCTCACTCA GTTTGTTGAA TCATTCACCA GGCAAAGGGC TGGCAGGGTC 
GCTGGCGGTA GGAATCTTCC AGTCGCAGTA GAGAAAGTAT CAAAGGCTTC AATTGACCAG 
AGCAGAGAGA TGAAATACCA GTCTTTTAAT GAGTATCGCA AACGTTTTCT GCTGAAGCCC 
TATGAATCAT TTGAGGAACT TACAGGAGAG AAGGAAATGG CTGCAGAGTT AGAAGCGCTC 
TATGGAGACA TAGATGCCAT GGAGTTTTAT CCCGCCCTTC TGGTAGAGAA GCCCCGTCCA 
GACGCCATCT TTGGGGAGAC CATGGTAGAA GCTGGAGCAC CATTCTCCCT GAAAGGACTT 
ATGGGTAATC CTATATGCTC TCCCGAGTAC TGGAAGCCTA GCACTTTCGG TGGAGAAGTA 
GGTTTTAAAA TCATCAACAC TGCCTCAATT CAGTCTCTCA TCTGCAGTAA CGTGAAAGGC 
TGTCCCTTTA CCTCATTCAG TGTTCAAGAT ACGCACCTCA CCAAAACGGT CACCATTAAT 
GCAAGCTCTT CCCACTCTGG ACTAGATGAT ATCAACCCCA CAGTCCTACT AAAGGAACGT 
TCAACCGAAC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGS2 ( XM_010813540.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811842.1 Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa45313
Clone ID Related Accession (Same CDS sequence)	XM_010813540.2
Accession Version	XM_010813540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prostaglandin G/H synthase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTAG GATTTGACCA GTATAAATGT GACTGTACCC GAACAGGATT CTACGGTGAA 
AACTGTACCA CACCCGAATT TCTGACAAGA ATAAAATTAC TCCTGAAACC CACTCCCAAC 
ACAGTGCACT ACATACTTAC CCACTTCAAA GGAGTCTGGA ACATTGTCAA TAAGATCTCC 
TTCCTGCGAA ATATGATTAT GAGATATGTG TTGACGTCGA GATCACATTT GATTGAGAGT 
CCACCAACTT ATAATGTGCA CTACAGCTAT AAAAGCTGGG AAGCCTTTTC TAACCTGTCT 
TATTATACCA GAGCTCTTCC TCCTGTGCCT GATGACTGCC CAACACCCAT GGGTGTGAAA 
GGGAGGAAAG AGCTTCCTGA TTCAAAAGAA GTTGTGAAAA AAGTACTTCT AAGAAGAAAG 
TTCATTCCTG ATCCCCAGGG CACAAATCTG ATGTTTGCAT TCTTTGCCCA GCACTTCACC 
CATCAATTTT TCAAGACAGA TTTTGAACGA GGACCAGCTT TCACTAAGGG AAAGAACCAT 
GGGGTGGACT TAAGTCACAT TTATGGTGAA TCTTTAGAGA GACAGCATAA GCTGCGCCTT 
TTCAAGGATG GAAAAATGAA ATATCAGATG ATTAATGGAG AGATGTATCC TCCCACAGTC 
AAAGATACTC AGGTCGAAAT GATCTACCCG CCTCATGTTC CTGAACACTT GAAGTTTGCT 
GTGGGCCAGG AAGTCTTTGG TCTGGTGCCT GGTCTGATGA TGTATGCCAC CATTTGGCTA 
CGGGAACACA ACAGAGTGTG TGATGTGCTT AAACAAGAGC ATCCAGAATG GGGCGATGAG 
CAGTTGTTCC AGACAAGCAG GCTAATCCTG ATAGGAGAAA CTATTAAGAT TGTGATTGAA 
GACTACGTAC AGCACTTGAG TGGCTATCAC TTCAAACTGA AGTTTGACCC AGAGCTGCTT 
TTCAACCAAC AGTTCCAGTA CCAGAACCGT ATTGCTGCTG AGTTTAACAC GCTCTACCAC 
TGGCATCCCC TTCTGCCTGA CGTCTTTCAG ATTGATGGTC AGGAGTACAA CTATCAGCAG 
TTTATCTATA ACAACTCTGT CTTACTGGAA CATGGTCTCA CTCAGTTTGT TGAATCATTC 
ACCAGGCAAA GGGCTGGCAG GGTCGCTGGC GGTAGGAATC TTCCAGTCGC AGTAGAGAAA 
GTATCAAAGG CTTCAATTGA CCAGAGCAGA GAGATGAAAT ACCAGTCTTT TAATGAGTAT 
CGCAAACGTT TTCTGCTGAA GCCCTATGAA TCATTTGAGG AACTTACAGG AGAGAAGGAA 
ATGGCTGCAG AGTTAGAAGC GCTCTATGGA GACATAGATG CCATGGAGTT TTATCCCGCC 
CTTCTGGTAG AGAAGCCCCG TCCAGACGCC ATCTTTGGGG AGACCATGGT AGAAGCTGGA 
GCACCATTCT CCCTGAAAGG ACTTATGGGT AATCCTATAT GCTCTCCCGA GTACTGGAAG 
CCTAGCACTT TCGGTGGAGA AGTAGGTTTT AAAATCATCA ACACTGCCTC AATTCAGTCT 
CTCATCTGCA GTAACGTGAA AGGCTGTCCC TTTACCTCAT TCAGTGTTCA AGATACGCAC 
CTCACCAAAA CGGTCACCAT TAATGCAAGC TCTTCCCACT CTGGACTAGA TGATATCAAC 
CCCACAGTCC TACTAAAGGA ACGTTCAACC GAACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811842.1
CDS	245..1963
Translation	MSVGFDQYKCDCTRTGFYGENCTTPEFLTRIKLLLKPTPNTVHYILTHFKGVWNIVNKISFLRNMIMRYVLTSRSHLIESPPTYNVHYSYKSWEAFSNLSYYTRALPPVPDDCPTPMGVKGRKELPDSKEVVKKVLLRRKFIPDPQGTNLMFAFFAQHFTHQFFKTDFERGPAFTKGKNHGVDLSHIYGESLERQHKLRLFKDGKMKYQMINGEMYPPTVKDTQVEMIYPPHVPEHLKFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNQQFQYQNRIAAEFNTLYHWHPLLPDVFQIDGQEYNYQQFIYNNSVLLEHGLTQFVESFTRQRAGRVAGGRNLPVAVEKVSKASIDQSREMKYQSFNEYRKRFLLKPYESFEELTGEKEMAAELEALYGDIDAMEFYPALLVEKPRPDAIFGETMVEAGAPFSLKGLMGNPICSPEYWKPSTFGGEVGFKIINTASIQSLICSNVKGCPFTSFSVQDTHLTKTVTINASSSHSGLDDINPTVLLKERSTEL

Target ORF information:

RefSeq Version	XM_010813540.2
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813540.2

ORF Insert Sequence:

ATGAGTGTAG GATTTGACCA GTATAAATGT GACTGTACCC GAACAGGATT CTACGGTGAA 
AACTGTACCA CACCCGAATT TCTGACAAGA ATAAAATTAC TCCTGAAACC CACTCCCAAC 
ACAGTGCACT ACATACTTAC CCACTTCAAA GGAGTCTGGA ACATTGTCAA TAAGATCTCC 
TTCCTGCGAA ATATGATTAT GAGATATGTG TTGACGTCGA GATCACATTT GATTGAGAGT 
CCACCAACTT ATAATGTGCA CTACAGCTAT AAAAGCTGGG AAGCCTTTTC TAACCTGTCT 
TATTATACCA GAGCTCTTCC TCCTGTGCCT GATGACTGCC CAACACCCAT GGGTGTGAAA 
GGGAGGAAAG AGCTTCCTGA TTCAAAAGAA GTTGTGAAAA AAGTACTTCT AAGAAGAAAG 
TTCATTCCTG ATCCCCAGGG CACAAATCTG ATGTTTGCAT TCTTTGCCCA GCACTTCACC 
CATCAATTTT TCAAGACAGA TTTTGAACGA GGACCAGCTT TCACTAAGGG AAAGAACCAT 
GGGGTGGACT TAAGTCACAT TTATGGTGAA TCTTTAGAGA GACAGCATAA GCTGCGCCTT 
TTCAAGGATG GAAAAATGAA ATATCAGATG ATTAATGGAG AGATGTATCC TCCCACAGTC 
AAAGATACTC AGGTCGAAAT GATCTACCCG CCTCATGTTC CTGAACACTT GAAGTTTGCT 
GTGGGCCAGG AAGTCTTTGG TCTGGTGCCT GGTCTGATGA TGTATGCCAC CATTTGGCTA 
CGGGAACACA ACAGAGTGTG TGATGTGCTT AAACAAGAGC ATCCAGAATG GGGCGATGAG 
CAGTTGTTCC AGACAAGCAG GCTAATCCTG ATAGGAGAAA CTATTAAGAT TGTGATTGAA 
GACTACGTAC AGCACTTGAG TGGCTATCAC TTCAAACTGA AGTTTGACCC AGAGCTGCTT 
TTCAACCAAC AGTTCCAGTA CCAGAACCGT ATTGCTGCTG AGTTTAACAC GCTCTACCAC 
TGGCATCCCC TTCTGCCTGA CGTCTTTCAG ATTGATGGTC AGGAGTACAA CTATCAGCAG 
TTTATCTATA ACAACTCTGT CTTACTGGAA CATGGTCTCA CTCAGTTTGT TGAATCATTC 
ACCAGGCAAA GGGCTGGCAG GGTCGCTGGC GGTAGGAATC TTCCAGTCGC AGTAGAGAAA 
GTATCAAAGG CTTCAATTGA CCAGAGCAGA GAGATGAAAT ACCAGTCTTT TAATGAGTAT 
CGCAAACGTT TTCTGCTGAA GCCCTATGAA TCATTTGAGG AACTTACAGG AGAGAAGGAA 
ATGGCTGCAG AGTTAGAAGC GCTCTATGGA GACATAGATG CCATGGAGTT TTATCCCGCC 
CTTCTGGTAG AGAAGCCCCG TCCAGACGCC ATCTTTGGGG AGACCATGGT AGAAGCTGGA 
GCACCATTCT CCCTGAAAGG ACTTATGGGT AATCCTATAT GCTCTCCCGA GTACTGGAAG 
CCTAGCACTT TCGGTGGAGA AGTAGGTTTT AAAATCATCA ACACTGCCTC AATTCAGTCT 
CTCATCTGCA GTAACGTGAA AGGCTGTCCC TTTACCTCAT TCAGTGTTCA AGATACGCAC 
CTCACCAAAA CGGTCACCAT TAATGCAAGC TCTTCCCACT CTGGACTAGA TGATATCAAC 
CCCACAGTCC TACTAAAGGA ACGTTCAACC GAACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTGS2 ( XM_015475265.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330751.1 Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X2, mRNA.

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CloneID	OBa93053
Clone ID Related Accession (Same CDS sequence)	XM_015475265.1
Accession Version	XM_015475265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prostaglandin G/H synthase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTTCC TCCAGATCTT TATCCAGCTT TATGACAGAG AAGAAAGAAC AAATCCTTGC 
TGTTCCCATC CATGCCAGAA TCGAGGTGTA TGTATGAGTG TAGGATTTGA CCAGTATAAA 
TGTGACTGTA CCCGAACAGG ATTCTACGGT GAAAACTGTA CCACACCCGA ATTTCTGACA 
AGAATAAAAT TACTCCTGAA ACCCACTCCC AACACAGTGC ACTACATACT TACCCACTTC 
AAAGGAGTCT GGAACATTGT CAATAAGATC TCCTTCCTGC GAAATATGAT TATGAGATAT 
GTGTTGACGT CGAGATCACA TTTGATTGAG AGTCCACCAA CTTATAATGT GCACTACAGC 
TATAAAAGCT GGGAAGCCTT TTCTAACCTG TCTTATTATA CCAGAGCTCT TCCTCCTGTG 
CCTGATGACT GCCCAACACC CATGGGTGTG AAAGGGAGGA AAGAGCTTCC TGATTCAAAA 
GAAGTTGTGA AAAAAGTACT TCTAAGAAGA AAGTTCATTC CTGATCCCCA GGGCACAAAT 
CTGATGTTTG CATTCTTTGC CCAGCACTTC ACCCATCAAT TTTTCAAGAC AGATTTTGAA 
CGAGGACCAG CTTTCACTAA GGGAAAGAAC CATGGGGTGG ACTTAAGTCA CATTTATGGT 
GAATCTTTAG AGAGACAGCA TAAGCTGCGC CTTTTCAAGG ATGGAAAAAT GAAATATCAG 
ATGATTAATG GAGAGATGTA TCCTCCCACA GTCAAAGATA CTCAGGTCGA AATGATCTAC 
CCGCCTCATG TTCCTGAACA CTTGAAGTTT GCTGTGGGCC AGGAAGTCTT TGGTCTGGTG 
CCTGGTCTGA TGATGTATGC CACCATTTGG CTACGGGAAC ACAACAGAGT GTGTGATGTG 
CTTAAACAAG AGCATCCAGA ATGGGGCGAT GAGCAGTTGT TCCAGACAAG CAGGCTAATC 
CTGATAGGAG AAACTATTAA GATTGTGATT GAAGACTACG TACAGCACTT GAGTGGCTAT 
CACTTCAAAC TGAAGTTTGA CCCAGAGCTG CTTTTCAACC AACAGTTCCA GTACCAGAAC 
CGTATTGCTG CTGAGTTTAA CACGCTCTAC CACTGGCATC CCCTTCTGCC TGACGTCTTT 
CAGATTGATG GTCAGGAGTA CAACTATCAG CAGTTTATCT ATAACAACTC TGTCTTACTG 
GAACATGGTC TCACTCAGTT TGTTGAATCA TTCACCAGGC AAAGGGCTGG CAGGGTCGCT 
GGCGGTAGGA ATCTTCCAGT CGCAGTAGAG AAAGTATCAA AGGCTTCAAT TGACCAGAGC 
AGAGAGATGA AATACCAGTC TTTTAATGAG TATCGCAAAC GTTTTCTGCT GAAGCCCTAT 
GAATCATTTG AGGAACTTAC AGGAGAGAAG GAAATGGCTG CAGAGTTAGA AGCGCTCTAT 
GGAGACATAG ATGCCATGGA GTTTTATCCC GCCCTTCTGG TAGAGAAGCC CCGTCCAGAC 
GCCATCTTTG GGGAGACCAT GGTAGAAGCT GGAGCACCAT TCTCCCTGAA AGGACTTATG 
GGTAATCCTA TATGCTCTCC CGAGTACTGG AAGCCTAGCA CTTTCGGTGG AGAAGTAGGT 
TTTAAAATCA TCAACACTGC CTCAATTCAG TCTCTCATCT GCAGTAACGT GAAAGGCTGT 
CCCTTTACCT CATTCAGTGT TCAAGATACG CACCTCACCA AAACGGTCAC CATTAATGCA 
AGCTCTTCCC ACTCTGGACT AGATGATATC AACCCCACAG TCCTACTAAA GGAACGTTCA 
ACCGAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330751.1
CDS	389..2200
Translation	MFFLQIFIQLYDREERTNPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCTTPEFLTRIKLLLKPTPNTVHYILTHFKGVWNIVNKISFLRNMIMRYVLTSRSHLIESPPTYNVHYSYKSWEAFSNLSYYTRALPPVPDDCPTPMGVKGRKELPDSKEVVKKVLLRRKFIPDPQGTNLMFAFFAQHFTHQFFKTDFERGPAFTKGKNHGVDLSHIYGESLERQHKLRLFKDGKMKYQMINGEMYPPTVKDTQVEMIYPPHVPEHLKFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNQQFQYQNRIAAEFNTLYHWHPLLPDVFQIDGQEYNYQQFIYNNSVLLEHGLTQFVESFTRQRAGRVAGGRNLPVAVEKVSKASIDQSREMKYQSFNEYRKRFLLKPYESFEELTGEKEMAAELEALYGDIDAMEFYPALLVEKPRPDAIFGETMVEAGAPFSLKGLMGNPICSPEYWKPSTFGGEVGFKIINTASIQSLICSNVKGCPFTSFSVQDTHLTKTVTINASSSHSGLDDINPTVLLKERSTEL

Target ORF information:

RefSeq Version	XM_015475265.1
Organism	Bos taurus(cattle)
Definition	Bos taurus prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475265.1

ORF Insert Sequence:

ATGTTCTTCC TCCAGATCTT TATCCAGCTT TATGACAGAG AAGAAAGAAC AAATCCTTGC 
TGTTCCCATC CATGCCAGAA TCGAGGTGTA TGTATGAGTG TAGGATTTGA CCAGTATAAA 
TGTGACTGTA CCCGAACAGG ATTCTACGGT GAAAACTGTA CCACACCCGA ATTTCTGACA 
AGAATAAAAT TACTCCTGAA ACCCACTCCC AACACAGTGC ACTACATACT TACCCACTTC 
AAAGGAGTCT GGAACATTGT CAATAAGATC TCCTTCCTGC GAAATATGAT TATGAGATAT 
GTGTTGACGT CGAGATCACA TTTGATTGAG AGTCCACCAA CTTATAATGT GCACTACAGC 
TATAAAAGCT GGGAAGCCTT TTCTAACCTG TCTTATTATA CCAGAGCTCT TCCTCCTGTG 
CCTGATGACT GCCCAACACC CATGGGTGTG AAAGGGAGGA AAGAGCTTCC TGATTCAAAA 
GAAGTTGTGA AAAAAGTACT TCTAAGAAGA AAGTTCATTC CTGATCCCCA GGGCACAAAT 
CTGATGTTTG CATTCTTTGC CCAGCACTTC ACCCATCAAT TTTTCAAGAC AGATTTTGAA 
CGAGGACCAG CTTTCACTAA GGGAAAGAAC CATGGGGTGG ACTTAAGTCA CATTTATGGT 
GAATCTTTAG AGAGACAGCA TAAGCTGCGC CTTTTCAAGG ATGGAAAAAT GAAATATCAG 
ATGATTAATG GAGAGATGTA TCCTCCCACA GTCAAAGATA CTCAGGTCGA AATGATCTAC 
CCGCCTCATG TTCCTGAACA CTTGAAGTTT GCTGTGGGCC AGGAAGTCTT TGGTCTGGTG 
CCTGGTCTGA TGATGTATGC CACCATTTGG CTACGGGAAC ACAACAGAGT GTGTGATGTG 
CTTAAACAAG AGCATCCAGA ATGGGGCGAT GAGCAGTTGT TCCAGACAAG CAGGCTAATC 
CTGATAGGAG AAACTATTAA GATTGTGATT GAAGACTACG TACAGCACTT GAGTGGCTAT 
CACTTCAAAC TGAAGTTTGA CCCAGAGCTG CTTTTCAACC AACAGTTCCA GTACCAGAAC 
CGTATTGCTG CTGAGTTTAA CACGCTCTAC CACTGGCATC CCCTTCTGCC TGACGTCTTT 
CAGATTGATG GTCAGGAGTA CAACTATCAG CAGTTTATCT ATAACAACTC TGTCTTACTG 
GAACATGGTC TCACTCAGTT TGTTGAATCA TTCACCAGGC AAAGGGCTGG CAGGGTCGCT 
GGCGGTAGGA ATCTTCCAGT CGCAGTAGAG AAAGTATCAA AGGCTTCAAT TGACCAGAGC 
AGAGAGATGA AATACCAGTC TTTTAATGAG TATCGCAAAC GTTTTCTGCT GAAGCCCTAT 
GAATCATTTG AGGAACTTAC AGGAGAGAAG GAAATGGCTG CAGAGTTAGA AGCGCTCTAT 
GGAGACATAG ATGCCATGGA GTTTTATCCC GCCCTTCTGG TAGAGAAGCC CCGTCCAGAC 
GCCATCTTTG GGGAGACCAT GGTAGAAGCT GGAGCACCAT TCTCCCTGAA AGGACTTATG 
GGTAATCCTA TATGCTCTCC CGAGTACTGG AAGCCTAGCA CTTTCGGTGG AGAAGTAGGT 
TTTAAAATCA TCAACACTGC CTCAATTCAG TCTCTCATCT GCAGTAACGT GAAAGGCTGT 
CCCTTTACCT CATTCAGTGT TCAAGATACG CACCTCACCA AAACGGTCAC CATTAATGCA 
AGCTCTTCCC ACTCTGGACT AGATGATATC AACCCCACAG TCCTACTAAA GGAACGTTCA 
ACCGAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle.
Animal reproduction science18754-63(2017 Dec)
Gao L,Liu B,Mao W,Gao R,Zhang S,Duritahala,Fu C,Shen Y,Zhang Y,Zhang N,Wu J,Deng Y,Wu X,Cao J

Endometrial mRNA expression of prostaglandin synthase enzymes PTGS 2, PTGFS and mPTGES 1 in repeat-breeding cows with cytologically determined endometritis.
Acta veterinaria Hungarica65(1)96-104(2017 03)
Janowski T,Bara?ski W,?ukasik K,Skar?y?ski D,Zdu?czyk S,Malinowska K

Decreased cyclooxygenase-2 gene expression and lactoferrin release in blood neutrophils of heifers during the calving period.
Veterinary immunology and immunopathology160(1-2)139-44(2014 Jul)
Hidalgo MA,Loncom?n CA,Hidalgo AI,Andrade V,Carretta MD,Burgos RA

REV-ERB? inhibits the PTGS2 expression in bovine uterus endometrium stromal and epithelial cells exposed to ovarian steroids.
The Journal of reproduction and development60(5)362-70(2014)
Isayama K,Chen H,Yamauchi N,Hattori MA

Altered expression of inflammation-associated genes in oviductal cells following follicular fluid exposure: implications for ovarian carcinogenesis.
Experimental biology and medicine (Maywood, N.J.)239(1)24-32(2014 Jan)
Lau A,Kollara A,St John E,Tone AA,Virtanen C,Greenblatt EM,King WA,Brown TJ

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PTGS2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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