PRKCA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKCA
Entrez Gene ID	282001
Full Name	protein kinase C alpha
Synonyms	PKRCA
General protein information	Preferred Names protein kinase C alpha Names protein kinase C alpha type PKC-A PKC-alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979614.1	XP_024835382.1	protein kinase C alpha type isoform X2
XM_024979613.1	XP_024835381.1	protein kinase C alpha type isoform X1
XM_024979615.1	XP_024835383.1	protein kinase C alpha type isoform X3
NM_174435.1	NP_776860.1	protein kinase C alpha type

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkca	NP_035231.2
Rattus norvegicus (Norway rat)	Prkca	NP_001099183.1
Drosophila melanogaster (fruit fly)	Pkc53E	NP_725626.1
Homo sapiens (human)	PRKCA	NP_002728.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012252	XP_320290.4
Canis lupus familiaris (dog)	PRKCA	XP_005624328.1
Bos taurus (cattle)	PRKCA	NP_776860.1
Gallus gallus (chicken)	PRKCA	NP_001012822.1
Danio rerio (zebrafish)	prkca	NP_001243170.1
	PRKCA	XP_002800615.1
Gallus gallus (chicken)	LOC101749801	XP_004946286.1
Caenorhabditis elegans (roundworm)	pkc-2	NP_001024518.1
Pan troglodytes (chimpanzee)	PRKCA	XP_003315734.1
Xenopus tropicalis (tropical clawed frog)	prkca	NP_001072752.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein kinase C and Src family kinases mediate angiotensin II-induced protein kinase D activation and acute aldosterone production.
Olala LO, Shapiro BA, Merchen TC, Wynn JJ, Bollag WB
Molecular and cellular endocrinology392(1-2)173-81(2014 Jul)

FRET study of the structural and kinetic effects of PKC phosphomimetic cardiac troponin T mutants on thin filament regulation.
Schlecht W, Zhou Z, Li KL, Rieck D, Ouyang Y, Dong WJ
Archives of biochemistry and biophysics550-5511-11(2014 May)

Role of PKC-? in NF-?B-MT1-MMP-mediated activation of proMMP-2 by TNF-? in pulmonary artery smooth muscle cells.
Roy S, Chakraborti T, Chowdhury A, Chakraborti S
Journal of biochemistry153(3)289-302(2013 Mar)

Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.
Haines RJ, Corbin KD, Pendleton LC, Eichler DC
The Journal of biological chemistry287(31)26168-76(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AngII activates PKD via a mechanism involving Src family kinases and PKC, to underlie increased aldosterone production.
Title: Protein kinase C and Src family kinases mediate angiotensin II-induced protein kinase D activation and acute aldosterone production.

Significant changes in thin filament Ca2+-sensitivity, structure and kinetics are brought about through PKC phosphorylation of cardiac troponin T.
Title: FRET study of the structural and kinetic effects of PKC phosphomimetic cardiac troponin T mutants on thin filament regulation.

Data indicate the involvement of PKC-alpha in proMMP-2 activation and inhibition of TIMP-2 expression by NF-kappaB-MT1-MMP-dependent and -independent pathway.
Title: Role of PKC-? in NF-?B-MT1-MMP-mediated activation of proMMP-2 by TNF-? in pulmonary artery smooth muscle cells.

calcium-dependent phosphorylation of argininosuccinate synthase Ser-328 is mediated by PKCalpha
Title: Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.

VDAC phosphorylation is an important determinant of its interaction with dimeric tubulin.
Title: Phosphorylation of voltage-dependent anion channel by serine/threonine kinases governs its interaction with tubulin.

The following PRKCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41326	NM_174435.1 Latest version!	Bos taurus protein kinase C alpha (PRKCA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa225974	XM_024979613.1 Latest version!	Bos taurus protein kinase C alpha (PRKCA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa225975	XM_024979614.1 Latest version!	Bos taurus protein kinase C alpha (PRKCA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa225976	XM_024979615.1 Latest version!	Bos taurus protein kinase C alpha (PRKCA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKCA ( NM_174435.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776860.1 Bos taurus protein kinase C alpha (PRKCA), mRNA.

Price & Availability↑

CloneID	OBa41326
Clone ID Related Accession (Same CDS sequence)	NM_174435.1
Accession Version	NM_174435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	protein kinase C alpha type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M13973.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M13973.1, BC151471.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGACG TCTTCCCGGC CGCCGAGCCG GCGGCGCCGC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CCGCTTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGGGCGGAT AAAGGACCCG ACACAGATGA CCCGAGGAGC 
AAGCACAAGT TCAAGATCCA CACGTATGGC AGCCCCACCT TCTGTGATCA CTGCGGCTCC 
CTGCTCTACG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGTGATAT GAACGTGCAC 
AAGCAGTGCG TGATCAACGT GCCCAGCCTC TGCGGGATGG ACCACACGGA GAAGAGGGGC 
CGCATCTACC TGAAGGCCGA GGTCACGGAT GAAAAGCTGC ACGTCACAGT ACGAGACGCG 
AAAAACCTAA TCCCTATGGA TCCAAATGGG CTTTCAGATC CTTACGTGAA GCTGAAGCTT 
ATTCCTGACC CCAAGAACGA GAGCAAACAG AAAACCAAGA CCATCCGCTC GACGCTGAAC 
CCCCGGTGGG ACGAGTCCTT CACGTTCAAA TTAAAACCTT CTGATAAAGA CCGGCGACTG 
TCCGAGGAAA TCTGGGACTG GGATCGAACC ACACGGAACG ACTTCATGGG GTCCCTTTCC 
TTTGGGGTCT CGGAGCTGAT GAAGATGCCG GCCAGCGGAT GGTACAAGCT GCTGAACCAA 
GAGGAGGGCG AGTACTACAA CGTGCCGATC CCCGAAGGCG ACGAGGAAGG CAATGTGGAG 
CTCAGGCAGA AATTCGAGAA AGCCAAGCTT GGCCCTGCCG GCAACAAAGT CATCAGTCCC 
TCCGAGGACA GGAGACAGCC TTCCAACAAC CTGGACAGAG TGAAGCTCAC GGACTTCAAC 
TTCCTCATGG TGCTGGGCAA AGGCAGCTTT GGGAAGGTGA TGCTGGCCGA CCGGAAGGGG 
ACAGAGGAGC TGTACGCCAT CAAGATCCTG AAGAAGGACG TGGTCATCCA GGACGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTC CTGGCGCTGC TCGACAAGCC GCCGTTCCTG 
ACGCAGCTGC ACTCCTGCTT CCAGACGGTG GACCGGCTGT ACTTCGTCAT GGAGTACGTC 
AACGGCGGGG ACCTCATGTA CCACATCCAG CAGGTCGGGA AGTTCAAGGA GCCGCAAGCA 
GTGTTCTATG CAGCAGAGAT TTCCATCGGG CTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATCGGGACC TGAAGTTAGA CAACGTCATG CTGGACTCGG AAGGACACAT TAAGATCGCG 
GACTTCGGGA TGTGCAAGGA GCACATGATG GACGGCGTCA CGACCAGGAC CTTCTGCGGG 
ACCCCCGACT ACATCGCCCC AGAGATAATC GCCTATCAGC CGTACGGGAA GTCCGTGGAC 
TGGTGGGCCT ACGGCGTCCT GTTGTACGAG ATGTTGGCCG GGCAGCCTCC GTTCGACGGC 
GAGGACGAGG ACGAGCTGTT CCAGTCCATC ATGGAGCACA ACGTCTCGTA CCCCAAGTCC 
TTGTCCAAGG AGGCCGTGTC CATCTGCAAA GGGCTGATGA CCAAGCACCC CGGGAAGCGG 
CTGGGCTGCG GGCCCGAGGG CGAGCGCGAC GTGCGGGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAGA AGCTGGAGAA CCGTGAGATC CAGCCACCCT TCAAGCCCAA AGTGTGCGGC 
AAAGGAGCAG AGAACTTTGA CAAGTTCTTC ACGCGAGGGC AGCCTGTCTT GACGCCGCCC 
GACCAGCTGG TCATCGCTAA CATCGACCAG TCTGATTTTG AAGGCTTCTC CTACGTCAAC 
CCCCAGTTCG TGCACCCCAT CCTGCAGAGC GCGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776860.1
CDS	51..2069
Translation	MADVFPAAEPAAPQDVANRFARKGALRQKNVHEVKNHRFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPRWDESFTFKLKPSDKDRRLSEEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRRQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPGKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Target ORF information:

RefSeq Version	NM_174435.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C alpha (PRKCA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174435.1

ORF Insert Sequence:

ATGGCTGACG TCTTCCCGGC CGCCGAGCCG GCGGCGCCGC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CCGCTTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGGGCGGAT AAAGGACCCG ACACAGATGA CCCGAGGAGC 
AAGCACAAGT TCAAGATCCA CACGTATGGC AGCCCCACCT TCTGTGATCA CTGCGGCTCC 
CTGCTCTACG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGTGATAT GAACGTGCAC 
AAGCAGTGCG TGATCAACGT GCCCAGCCTC TGCGGGATGG ACCACACGGA GAAGAGGGGC 
CGCATCTACC TGAAGGCCGA GGTCACGGAT GAAAAGCTGC ACGTCACAGT ACGAGACGCG 
AAAAACCTAA TCCCTATGGA TCCAAATGGG CTTTCAGATC CTTACGTGAA GCTGAAGCTT 
ATTCCTGACC CCAAGAACGA GAGCAAACAG AAAACCAAGA CCATCCGCTC GACGCTGAAC 
CCCCGGTGGG ACGAGTCCTT CACGTTCAAA TTAAAACCTT CTGATAAAGA CCGGCGACTG 
TCCGAGGAAA TCTGGGACTG GGATCGAACC ACACGGAACG ACTTCATGGG GTCCCTTTCC 
TTTGGGGTCT CGGAGCTGAT GAAGATGCCG GCCAGCGGAT GGTACAAGCT GCTGAACCAA 
GAGGAGGGCG AGTACTACAA CGTGCCGATC CCCGAAGGCG ACGAGGAAGG CAATGTGGAG 
CTCAGGCAGA AATTCGAGAA AGCCAAGCTT GGCCCTGCCG GCAACAAAGT CATCAGTCCC 
TCCGAGGACA GGAGACAGCC TTCCAACAAC CTGGACAGAG TGAAGCTCAC GGACTTCAAC 
TTCCTCATGG TGCTGGGCAA AGGCAGCTTT GGGAAGGTGA TGCTGGCCGA CCGGAAGGGG 
ACAGAGGAGC TGTACGCCAT CAAGATCCTG AAGAAGGACG TGGTCATCCA GGACGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTC CTGGCGCTGC TCGACAAGCC GCCGTTCCTG 
ACGCAGCTGC ACTCCTGCTT CCAGACGGTG GACCGGCTGT ACTTCGTCAT GGAGTACGTC 
AACGGCGGGG ACCTCATGTA CCACATCCAG CAGGTCGGGA AGTTCAAGGA GCCGCAAGCA 
GTGTTCTATG CAGCAGAGAT TTCCATCGGG CTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATCGGGACC TGAAGTTAGA CAACGTCATG CTGGACTCGG AAGGACACAT TAAGATCGCG 
GACTTCGGGA TGTGCAAGGA GCACATGATG GACGGCGTCA CGACCAGGAC CTTCTGCGGG 
ACCCCCGACT ACATCGCCCC AGAGATAATC GCCTATCAGC CGTACGGGAA GTCCGTGGAC 
TGGTGGGCCT ACGGCGTCCT GTTGTACGAG ATGTTGGCCG GGCAGCCTCC GTTCGACGGC 
GAGGACGAGG ACGAGCTGTT CCAGTCCATC ATGGAGCACA ACGTCTCGTA CCCCAAGTCC 
TTGTCCAAGG AGGCCGTGTC CATCTGCAAA GGGCTGATGA CCAAGCACCC CGGGAAGCGG 
CTGGGCTGCG GGCCCGAGGG CGAGCGCGAC GTGCGGGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAGA AGCTGGAGAA CCGTGAGATC CAGCCACCCT TCAAGCCCAA AGTGTGCGGC 
AAAGGAGCAG AGAACTTTGA CAAGTTCTTC ACGCGAGGGC AGCCTGTCTT GACGCCGCCC 
GACCAGCTGG TCATCGCTAA CATCGACCAG TCTGATTTTG AAGGCTTCTC CTACGTCAAC 
CCCCAGTTCG TGCACCCCAT CCTGCAGAGC GCGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCA ( XM_024979613.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835381.1 Bos taurus protein kinase C alpha (PRKCA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa225974
Clone ID Related Accession (Same CDS sequence)	XM_024979613.1
Accession Version	XM_024979613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C alpha type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TCTTCCCGGC CGCCGAGCCG GCGGCGCCGC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CCGCTTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGGGCGGAT AAAGGACCCG ACACAGATGA CCCGAGGAGC 
AAGCACAAGT TCAAGATCCA CACGTATGGC AGCCCCACCT TCTGTGATCA CTGCGGCTCC 
CTGCTCTACG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGTGATAT GAACGTGCAC 
AAGCAGTGCG TGATCAACGT GCCCAGCCTC TGCGGGATGG ACCACACGGA GAAGAGGGGC 
CGCATCTACC TGAAGGCCGA GGTCACGGAT GAAAAGCTGC ACGTCACAGT ACGAGACGCG 
AAAAACCTAA TCCCTATGGA TCCAAATGGG CTTTCAGATC CTTACGTGAA GCTGAAGCTT 
ATTCCTGACC CCAAGAACGA GAGCAAACAG AAAACCAAGA CCATCCGCTC GACGCTGAAC 
CCCCGGTGGG ACGAGTCCTT CACGTTCAAA TTAAAACCTT CTGATAAAGA CCGGCGACTG 
TCTGTGGAAA TCTGGGACTG GGATCGAACC ACACGGAACG ACTTTATGGG GTCCCTTTCC 
TTTGGGGTCT CGGAGCTGAT GAAGATGCCG GCCAGCGGAT GGTACAAGCT GCTGAACCAA 
GAGGAGGGCG AGTACTACAA CGTGCCGATC CCCGAAGGCG ACGAGGAAGG CAACGTGGAG 
CTCAGGCAGA AATTCGAGAA AGCCAAGCTT GGCCCTGCCG GCAACAAAGT CATCAGTCCC 
TCCGAGGACA GGAGACAGCC TTCCAACAAC CTGGACAGAG TGAAGCTCAC GGACTTCAAC 
TTCCTCATGG TGCTGGGCAA AGGCAGCTTT GGGAAGGTGA TGCTGGCCGA CCGGAAGGGG 
ACAGAGGAGC TGTACGCCAT CAAGATCCTG AAGAAGGACG TGGTCATCCA GGACGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTC CTGGCGCTGC TCGACAAGCC GCCGTTCCTG 
ACGCAGCTGC ACTCCTGCTT CCAGACGGTG GACCGGCTGT ACTTCGTCAT GGAGTACGTC 
AACGGCGGGG ACCTCATGTA CCACATCCAG CAGGTCGGGA AGTTCAAGGA GCCGCAAGCA 
GTGTTCTATG CAGCAGAGAT TTCCATCGGG CTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATCGGGACC TGAAGTTAGA CAACGTCATG CTGGACTCGG AAGGACACAT TAAGATCGCG 
GACTTCGGGA TGTGCAAGGA GCACATGATG GACGGCGTCA CGACCAGGAC CTTCTGCGGG 
ACCCCCGACT ACATCGCCCC AGAGATAATC GCCTATCAGC CGTACGGGAA GTCCGTGGAC 
TGGTGGGCCT ACGGCGTCCT GTTGTACGAG ATGTTGGCCG GGCAGCCTCC GTTCGACGGC 
GAGGACGAGG ACGAGCTGTT CCAGTCCATC ATGGAGCACA ACGTCTCGTA CCCCAAGTCC 
TTGTCCAAGG AGGCCGTGTC CATCTGCAAA GGGCTGATGA CCAAGCACCC CGGGAAGCGG 
CTGGGCTGCG GGCCCGAGGG CGAGCGCGAC GTGCGGGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAGA AGCTGGAGAA CCGTGAGATC CAGCCACCCT TCAAGCCCAA AGTGACTTTC 
TGCACCAAAA CGCACTGGCT TCAGTGGGCG TCCAGGCCTT CGTGCGGCAA AGGAGCAGAG 
AACTTTGACA AGTTCTTCAC GCGAGGGCAG CCTGTCTTGA CGCCGCCCGA CCAGCTGGTC 
ATCGCTAACA TCGACCAGTC TGATTTTGAA GGCTTCTCCT ACGTCAACCC CCAGTTCGTG 
CACCCCATCC TGCAGAGCGC GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835381.1
CDS	110..2176
Translation	MADVFPAAEPAAPQDVANRFARKGALRQKNVHEVKNHRFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPRWDESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRRQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPGKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVTFCTKTHWLQWASRPSCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Target ORF information:

RefSeq Version	XM_024979613.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C alpha (PRKCA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979613.1

ORF Insert Sequence:

ATGGCTGACG TCTTCCCGGC CGCCGAGCCG GCGGCGCCGC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CCGCTTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGGGCGGAT AAAGGACCCG ACACAGATGA CCCGAGGAGC 
AAGCACAAGT TCAAGATCCA CACGTATGGC AGCCCCACCT TCTGTGATCA CTGCGGCTCC 
CTGCTCTACG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGTGATAT GAACGTGCAC 
AAGCAGTGCG TGATCAACGT GCCCAGCCTC TGCGGGATGG ACCACACGGA GAAGAGGGGC 
CGCATCTACC TGAAGGCCGA GGTCACGGAT GAAAAGCTGC ACGTCACAGT ACGAGACGCG 
AAAAACCTAA TCCCTATGGA TCCAAATGGG CTTTCAGATC CTTACGTGAA GCTGAAGCTT 
ATTCCTGACC CCAAGAACGA GAGCAAACAG AAAACCAAGA CCATCCGCTC GACGCTGAAC 
CCCCGGTGGG ACGAGTCCTT CACGTTCAAA TTAAAACCTT CTGATAAAGA CCGGCGACTG 
TCTGTGGAAA TCTGGGACTG GGATCGAACC ACACGGAACG ACTTTATGGG GTCCCTTTCC 
TTTGGGGTCT CGGAGCTGAT GAAGATGCCG GCCAGCGGAT GGTACAAGCT GCTGAACCAA 
GAGGAGGGCG AGTACTACAA CGTGCCGATC CCCGAAGGCG ACGAGGAAGG CAACGTGGAG 
CTCAGGCAGA AATTCGAGAA AGCCAAGCTT GGCCCTGCCG GCAACAAAGT CATCAGTCCC 
TCCGAGGACA GGAGACAGCC TTCCAACAAC CTGGACAGAG TGAAGCTCAC GGACTTCAAC 
TTCCTCATGG TGCTGGGCAA AGGCAGCTTT GGGAAGGTGA TGCTGGCCGA CCGGAAGGGG 
ACAGAGGAGC TGTACGCCAT CAAGATCCTG AAGAAGGACG TGGTCATCCA GGACGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTC CTGGCGCTGC TCGACAAGCC GCCGTTCCTG 
ACGCAGCTGC ACTCCTGCTT CCAGACGGTG GACCGGCTGT ACTTCGTCAT GGAGTACGTC 
AACGGCGGGG ACCTCATGTA CCACATCCAG CAGGTCGGGA AGTTCAAGGA GCCGCAAGCA 
GTGTTCTATG CAGCAGAGAT TTCCATCGGG CTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATCGGGACC TGAAGTTAGA CAACGTCATG CTGGACTCGG AAGGACACAT TAAGATCGCG 
GACTTCGGGA TGTGCAAGGA GCACATGATG GACGGCGTCA CGACCAGGAC CTTCTGCGGG 
ACCCCCGACT ACATCGCCCC AGAGATAATC GCCTATCAGC CGTACGGGAA GTCCGTGGAC 
TGGTGGGCCT ACGGCGTCCT GTTGTACGAG ATGTTGGCCG GGCAGCCTCC GTTCGACGGC 
GAGGACGAGG ACGAGCTGTT CCAGTCCATC ATGGAGCACA ACGTCTCGTA CCCCAAGTCC 
TTGTCCAAGG AGGCCGTGTC CATCTGCAAA GGGCTGATGA CCAAGCACCC CGGGAAGCGG 
CTGGGCTGCG GGCCCGAGGG CGAGCGCGAC GTGCGGGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAGA AGCTGGAGAA CCGTGAGATC CAGCCACCCT TCAAGCCCAA AGTGACTTTC 
TGCACCAAAA CGCACTGGCT TCAGTGGGCG TCCAGGCCTT CGTGCGGCAA AGGAGCAGAG 
AACTTTGACA AGTTCTTCAC GCGAGGGCAG CCTGTCTTGA CGCCGCCCGA CCAGCTGGTC 
ATCGCTAACA TCGACCAGTC TGATTTTGAA GGCTTCTCCT ACGTCAACCC CCAGTTCGTG 
CACCCCATCC TGCAGAGCGC GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCA ( XM_024979614.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835382.1 Bos taurus protein kinase C alpha (PRKCA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa225975
Clone ID Related Accession (Same CDS sequence)	XM_024979614.1
Accession Version	XM_024979614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C alpha type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACG TCTTCCCGGC CGCCGAGCCG GCGGCGCCGC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CCGCTTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGGGCGGAT AAAGGACCCG ACACAGATGA CCCGAGGAGC 
AAGCACAAGT TCAAGATCCA CACGTATGGC AGCCCCACCT TCTGTGATCA CTGCGGCTCC 
CTGCTCTACG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGTGATAT GAACGTGCAC 
AAGCAGTGCG TGATCAACGT GCCCAGCCTC TGCGGGATGG ACCACACGGA GAAGAGGGGC 
CGCATCTACC TGAAGGCCGA GGTCACGGAT GAAAAGCTGC ACGTCACAGT ACGAGACGCG 
AAAAACCTAA TCCCTATGGA TCCAAATGGG CTTTCAGATC CTTACGTGAA GCTGAAGCTT 
ATTCCTGACC CCAAGAACGA GAGCAAACAG AAAACCAAGA CCATCCGCTC GACGCTGAAC 
CCCCGGTGGG ACGAGTCCTT CACGTTCAAA TTAAAACCTT CTGATAAAGA CCGGCGACTG 
TCTGTGGAAA TCTGGGACTG GGATCGAACC ACACGGAACG ACTTTATGGG GTCCCTTTCC 
TTTGGGGTCT CGGAGCTGAT GAAGATGCCG GCCAGCGGAT GGTACAAGCT GCTGAACCAA 
GAGGAGGGCG AGTACTACAA CGTGCCGATC CCCGAAGGCG ACGAGGAAGG CAACGTGGAG 
CTCAGGCAGA AATTCGAGAA AGCCAAGCTT GGCCCTGCCG GCAACAAAGT CATCAGTCCC 
TCCGAGGACA GGAGACAGCC TTCCAACAAC CTGGACAGAG TGAAGCTCAC GGACTTCAAC 
TTCCTCATGG TGCTGGGCAA AGGCAGCTTT GGGAAGGTGA TGCTGGCCGA CCGGAAGGGG 
ACAGAGGAGC TGTACGCCAT CAAGATCCTG AAGAAGGACG TGGTCATCCA GGACGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTC CTGGCGCTGC TCGACAAGCC GCCGTTCCTG 
ACGCAGCTGC ACTCCTGCTT CCAGACGGTG GACCGGCTGT ACTTCGTCAT GGAGTACGTC 
AACGGCGGGG ACCTCATGTA CCACATCCAG CAGGTCGGGA AGTTCAAGGA GCCGCAAGCA 
GTGTTCTATG CAGCAGAGAT TTCCATCGGG CTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATCGGGACC TGAAGTTAGA CAACGTCATG CTGGACTCGG AAGGACACAT TAAGATCGCG 
GACTTCGGGA TGTGCAAGGA GCACATGATG GACGGCGTCA CGACCAGGAC CTTCTGCGGG 
ACCCCCGACT ACATCGCCCC AGAGATAATC GCCTATCAGC CGTACGGGAA GTCCGTGGAC 
TGGTGGGCCT ACGGCGTCCT GTTGTACGAG ATGTTGGCCG GGCAGCCTCC GTTCGACGGC 
GAGGACGAGG ACGAGCTGTT CCAGTCCATC ATGGAGCACA ACGTCTCGTA CCCCAAGTCC 
TTGTCCAAGG AGGCCGTGTC CATCTGCAAA GGGCTGATGA CCAAGCACCC CGGGAAGCGG 
CTGGGCTGCG GGCCCGAGGG CGAGCGCGAC GTGCGGGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAGA AGCTGGAGAA CCGTGAGATC CAGCCACCCT TCAAGCCCAA AGTGCGAGGT 
GGCGATCATG GTCCCCAGTT TACAGTCCAG AACACTGAGG CACAGAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835382.1
CDS	1..1914
Translation	MADVFPAAEPAAPQDVANRFARKGALRQKNVHEVKNHRFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPRWDESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRRQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPGKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVRGGDHGPQFTVQNTEAQRG

Target ORF information:

RefSeq Version	XM_024979614.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C alpha (PRKCA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979614.1

ORF Insert Sequence:

ATGGCTGACG TCTTCCCGGC CGCCGAGCCG GCGGCGCCGC AGGACGTGGC CAACCGCTTC 
GCCCGCAAAG GGGCGCTGAG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CCGCTTCATC 
GCGCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGGTTT 
GGGAAACAAG GCTTCCAGTG CCAAGTTTGC TGTTTTGTGG TTCACAAGAG GTGCCATGAA 
TTTGTTACTT TTTCTTGTCC GGGGGCGGAT AAAGGACCCG ACACAGATGA CCCGAGGAGC 
AAGCACAAGT TCAAGATCCA CACGTATGGC AGCCCCACCT TCTGTGATCA CTGCGGCTCC 
CTGCTCTACG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGTGATAT GAACGTGCAC 
AAGCAGTGCG TGATCAACGT GCCCAGCCTC TGCGGGATGG ACCACACGGA GAAGAGGGGC 
CGCATCTACC TGAAGGCCGA GGTCACGGAT GAAAAGCTGC ACGTCACAGT ACGAGACGCG 
AAAAACCTAA TCCCTATGGA TCCAAATGGG CTTTCAGATC CTTACGTGAA GCTGAAGCTT 
ATTCCTGACC CCAAGAACGA GAGCAAACAG AAAACCAAGA CCATCCGCTC GACGCTGAAC 
CCCCGGTGGG ACGAGTCCTT CACGTTCAAA TTAAAACCTT CTGATAAAGA CCGGCGACTG 
TCTGTGGAAA TCTGGGACTG GGATCGAACC ACACGGAACG ACTTTATGGG GTCCCTTTCC 
TTTGGGGTCT CGGAGCTGAT GAAGATGCCG GCCAGCGGAT GGTACAAGCT GCTGAACCAA 
GAGGAGGGCG AGTACTACAA CGTGCCGATC CCCGAAGGCG ACGAGGAAGG CAACGTGGAG 
CTCAGGCAGA AATTCGAGAA AGCCAAGCTT GGCCCTGCCG GCAACAAAGT CATCAGTCCC 
TCCGAGGACA GGAGACAGCC TTCCAACAAC CTGGACAGAG TGAAGCTCAC GGACTTCAAC 
TTCCTCATGG TGCTGGGCAA AGGCAGCTTT GGGAAGGTGA TGCTGGCCGA CCGGAAGGGG 
ACAGAGGAGC TGTACGCCAT CAAGATCCTG AAGAAGGACG TGGTCATCCA GGACGACGAC 
GTGGAGTGCA CCATGGTGGA GAAGCGGGTC CTGGCGCTGC TCGACAAGCC GCCGTTCCTG 
ACGCAGCTGC ACTCCTGCTT CCAGACGGTG GACCGGCTGT ACTTCGTCAT GGAGTACGTC 
AACGGCGGGG ACCTCATGTA CCACATCCAG CAGGTCGGGA AGTTCAAGGA GCCGCAAGCA 
GTGTTCTATG CAGCAGAGAT TTCCATCGGG CTGTTCTTTC TTCATAAAAG AGGAATCATT 
TATCGGGACC TGAAGTTAGA CAACGTCATG CTGGACTCGG AAGGACACAT TAAGATCGCG 
GACTTCGGGA TGTGCAAGGA GCACATGATG GACGGCGTCA CGACCAGGAC CTTCTGCGGG 
ACCCCCGACT ACATCGCCCC AGAGATAATC GCCTATCAGC CGTACGGGAA GTCCGTGGAC 
TGGTGGGCCT ACGGCGTCCT GTTGTACGAG ATGTTGGCCG GGCAGCCTCC GTTCGACGGC 
GAGGACGAGG ACGAGCTGTT CCAGTCCATC ATGGAGCACA ACGTCTCGTA CCCCAAGTCC 
TTGTCCAAGG AGGCCGTGTC CATCTGCAAA GGGCTGATGA CCAAGCACCC CGGGAAGCGG 
CTGGGCTGCG GGCCCGAGGG CGAGCGCGAC GTGCGGGAGC ATGCCTTCTT CCGGAGGATC 
GACTGGGAGA AGCTGGAGAA CCGTGAGATC CAGCCACCCT TCAAGCCCAA AGTGCGAGGT 
GGCGATCATG GTCCCCAGTT TACAGTCCAG AACACTGAGG CACAGAGAGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCA ( XM_024979615.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835383.1 Bos taurus protein kinase C alpha (PRKCA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa225976
Clone ID Related Accession (Same CDS sequence)	XM_024979615.1
Accession Version	XM_024979615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C alpha type isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG TTTGCTGTTT TGTGGTTCAC AAGAGGTGCC ATGAATTTGT TACTTTTTCT 
TGTCCGGGGG CGGATAAAGG ACCCGACACA GATGACCCGA GGAGCAAGCA CAAGTTCAAG 
ATCCACACGT ATGGCAGCCC CACCTTCTGT GATCACTGCG GCTCCCTGCT CTACGGACTC 
ATCCACCAGG GGATGAAATG TGACACCTGT GATATGAACG TGCACAAGCA GTGCGTGATC 
AACGTGCCCA GCCTCTGCGG GATGGACCAC ACGGAGAAGA GGGGCCGCAT CTACCTGAAG 
GCCGAGGTCA CGGATGAAAA GCTGCACGTC ACAGTACGAG ACGCGAAAAA CCTAATCCCT 
ATGGATCCAA ATGGGCTTTC AGATCCTTAC GTGAAGCTGA AGCTTATTCC TGACCCCAAG 
AACGAGAGCA AACAGAAAAC CAAGACCATC CGCTCGACGC TGAACCCCCG GTGGGACGAG 
TCCTTCACGT TCAAATTAAA ACCTTCTGAT AAAGACCGGC GACTGTCTGT GGAAATCTGG 
GACTGGGATC GAACCACACG GAACGACTTT ATGGGGTCCC TTTCCTTTGG GGTCTCGGAG 
CTGATGAAGA TGCCGGCCAG CGGATGGTAC AAGCTGCTGA ACCAAGAGGA GGGCGAGTAC 
TACAACGTGC CGATCCCCGA AGGCGACGAG GAAGGCAACG TGGAGCTCAG GCAGAAATTC 
GAGAAAGCCA AGCTTGGCCC TGCCGGCAAC AAAGTCATCA GTCCCTCCGA GGACAGGAGA 
CAGCCTTCCA ACAACCTGGA CAGAGTGAAG CTCACGGACT TCAACTTCCT CATGGTGCTG 
GGCAAAGGCA GCTTTGGGAA GGTGATGCTG GCCGACCGGA AGGGGACAGA GGAGCTGTAC 
GCCATCAAGA TCCTGAAGAA GGACGTGGTC ATCCAGGACG ACGACGTGGA GTGCACCATG 
GTGGAGAAGC GGGTCCTGGC GCTGCTCGAC AAGCCGCCGT TCCTGACGCA GCTGCACTCC 
TGCTTCCAGA CGGTGGACCG GCTGTACTTC GTCATGGAGT ACGTCAACGG CGGGGACCTC 
ATGTACCACA TCCAGCAGGT CGGGAAGTTC AAGGAGCCGC AAGCAGTGTT CTATGCAGCA 
GAGATTTCCA TCGGGCTGTT CTTTCTTCAT AAAAGAGGAA TCATTTATCG GGACCTGAAG 
TTAGACAACG TCATGCTGGA CTCGGAAGGA CACATTAAGA TCGCGGACTT CGGGATGTGC 
AAGGAGCACA TGATGGACGG CGTCACGACC AGGACCTTCT GCGGGACCCC CGACTACATC 
GCCCCAGAGA TAATCGCCTA TCAGCCGTAC GGGAAGTCCG TGGACTGGTG GGCCTACGGC 
GTCCTGTTGT ACGAGATGTT GGCCGGGCAG CCTCCGTTCG ACGGCGAGGA CGAGGACGAG 
CTGTTCCAGT CCATCATGGA GCACAACGTC TCGTACCCCA AGTCCTTGTC CAAGGAGGCC 
GTGTCCATCT GCAAAGGGCT GATGACCAAG CACCCCGGGA AGCGGCTGGG CTGCGGGCCC 
GAGGGCGAGC GCGACGTGCG GGAGCATGCC TTCTTCCGGA GGATCGACTG GGAGAAGCTG 
GAGAACCGTG AGATCCAGCC ACCCTTCAAG CCCAAAGTGA CTTTCTGCAC CAAAACGCAC 
TGGCTTCAGT GGGCGTCCAG GCCTTCGTGC GGCAAAGGAG CAGAGAACTT TGACAAGTTC 
TTCACGCGAG GGCAGCCTGT CTTGACGCCG CCCGACCAGC TGGTCATCGC TAACATCGAC 
CAGTCTGATT TTGAAGGCTT CTCCTACGTC AACCCCCAGT TCGTGCACCC CATCCTGCAG 
AGCGCGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835383.1
CDS	16..1887
Translation	MAEVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGSLLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPRWDESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNVELRQKFEKAKLGPAGNKVISPSEDRRQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPGKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVTFCTKTHWLQWASRPSCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Target ORF information:

RefSeq Version	XM_024979615.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C alpha (PRKCA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979615.1

ORF Insert Sequence:

ATGGCAGAGG TTTGCTGTTT TGTGGTTCAC AAGAGGTGCC ATGAATTTGT TACTTTTTCT 
TGTCCGGGGG CGGATAAAGG ACCCGACACA GATGACCCGA GGAGCAAGCA CAAGTTCAAG 
ATCCACACGT ATGGCAGCCC CACCTTCTGT GATCACTGCG GCTCCCTGCT CTACGGACTC 
ATCCACCAGG GGATGAAATG TGACACCTGT GATATGAACG TGCACAAGCA GTGCGTGATC 
AACGTGCCCA GCCTCTGCGG GATGGACCAC ACGGAGAAGA GGGGCCGCAT CTACCTGAAG 
GCCGAGGTCA CGGATGAAAA GCTGCACGTC ACAGTACGAG ACGCGAAAAA CCTAATCCCT 
ATGGATCCAA ATGGGCTTTC AGATCCTTAC GTGAAGCTGA AGCTTATTCC TGACCCCAAG 
AACGAGAGCA AACAGAAAAC CAAGACCATC CGCTCGACGC TGAACCCCCG GTGGGACGAG 
TCCTTCACGT TCAAATTAAA ACCTTCTGAT AAAGACCGGC GACTGTCTGT GGAAATCTGG 
GACTGGGATC GAACCACACG GAACGACTTT ATGGGGTCCC TTTCCTTTGG GGTCTCGGAG 
CTGATGAAGA TGCCGGCCAG CGGATGGTAC AAGCTGCTGA ACCAAGAGGA GGGCGAGTAC 
TACAACGTGC CGATCCCCGA AGGCGACGAG GAAGGCAACG TGGAGCTCAG GCAGAAATTC 
GAGAAAGCCA AGCTTGGCCC TGCCGGCAAC AAAGTCATCA GTCCCTCCGA GGACAGGAGA 
CAGCCTTCCA ACAACCTGGA CAGAGTGAAG CTCACGGACT TCAACTTCCT CATGGTGCTG 
GGCAAAGGCA GCTTTGGGAA GGTGATGCTG GCCGACCGGA AGGGGACAGA GGAGCTGTAC 
GCCATCAAGA TCCTGAAGAA GGACGTGGTC ATCCAGGACG ACGACGTGGA GTGCACCATG 
GTGGAGAAGC GGGTCCTGGC GCTGCTCGAC AAGCCGCCGT TCCTGACGCA GCTGCACTCC 
TGCTTCCAGA CGGTGGACCG GCTGTACTTC GTCATGGAGT ACGTCAACGG CGGGGACCTC 
ATGTACCACA TCCAGCAGGT CGGGAAGTTC AAGGAGCCGC AAGCAGTGTT CTATGCAGCA 
GAGATTTCCA TCGGGCTGTT CTTTCTTCAT AAAAGAGGAA TCATTTATCG GGACCTGAAG 
TTAGACAACG TCATGCTGGA CTCGGAAGGA CACATTAAGA TCGCGGACTT CGGGATGTGC 
AAGGAGCACA TGATGGACGG CGTCACGACC AGGACCTTCT GCGGGACCCC CGACTACATC 
GCCCCAGAGA TAATCGCCTA TCAGCCGTAC GGGAAGTCCG TGGACTGGTG GGCCTACGGC 
GTCCTGTTGT ACGAGATGTT GGCCGGGCAG CCTCCGTTCG ACGGCGAGGA CGAGGACGAG 
CTGTTCCAGT CCATCATGGA GCACAACGTC TCGTACCCCA AGTCCTTGTC CAAGGAGGCC 
GTGTCCATCT GCAAAGGGCT GATGACCAAG CACCCCGGGA AGCGGCTGGG CTGCGGGCCC 
GAGGGCGAGC GCGACGTGCG GGAGCATGCC TTCTTCCGGA GGATCGACTG GGAGAAGCTG 
GAGAACCGTG AGATCCAGCC ACCCTTCAAG CCCAAAGTGA CTTTCTGCAC CAAAACGCAC 
TGGCTTCAGT GGGCGTCCAG GCCTTCGTGC GGCAAAGGAG CAGAGAACTT TGACAAGTTC 
TTCACGCGAG GGCAGCCTGT CTTGACGCCG CCCGACCAGC TGGTCATCGC TAACATCGAC 
CAGTCTGATT TTGAAGGCTT CTCCTACGTC AACCCCCAGT TCGTGCACCC CATCCTGCAG 
AGCGCGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein kinase C and Src family kinases mediate angiotensin II-induced protein kinase D activation and acute aldosterone production.
Molecular and cellular endocrinology392(1-2)173-81(2014 Jul)
Olala LO,Shapiro BA,Merchen TC,Wynn JJ,Bollag WB

FRET study of the structural and kinetic effects of PKC phosphomimetic cardiac troponin T mutants on thin filament regulation.
Archives of biochemistry and biophysics550-5511-11(2014 May)
Schlecht W,Zhou Z,Li KL,Rieck D,Ouyang Y,Dong WJ

Role of PKC-? in NF-?B-MT1-MMP-mediated activation of proMMP-2 by TNF-? in pulmonary artery smooth muscle cells.
Journal of biochemistry153(3)289-302(2013 Mar)
Roy S,Chakraborti T,Chowdhury A,Chakraborti S

Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.
The Journal of biological chemistry287(31)26168-76(2012 Jul)
Haines RJ,Corbin KD,Pendleton LC,Eichler DC

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PRKCA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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