PLCB4 cDNA ORF clone, Bos taurus(cattle)

The following PLCB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa221441 NM_001166510.2
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221438 XM_025000311.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221438 XM_025000310.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221438 XM_025000312.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221439 XM_025000314.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221439 XM_025000313.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221440 XM_025000315.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221441 XM_025000316.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221442 XM_025000317.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221443 XM_025000318.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221444 XM_025000319.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa221445 XM_025000320.1
Latest version!
Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa44452 NM_001166510.1
Latest version!
Bos taurus phospholipase C, beta 4 (PLCB4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa221441
    Clone ID Related Accession (Same CDS sequence) XM_025000316.1 , NM_001166510.2
    Accession Version NM_001166510.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000013.1. On May 30, 2018 this sequence version replaced NM_001166510.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5721694.2368.1, SRR5765710.4612.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001159982.1
    CDS1..3585
    Translation

    Target ORF information:

    RefSeq Version NM_001166510.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001166510.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221438
    Clone ID Related Accession (Same CDS sequence) XM_025000312.1 , XM_025000311.1 , XM_025000310.1
    Accession Version XM_025000312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA CGCAGTGTCC
    CAAACGCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG
    ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856080.1
    CDS430..4068
    Translation

    Target ORF information:

    RefSeq Version XM_025000312.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA CGCAGTGTCC
    CAAACGCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG
    ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221438
    Clone ID Related Accession (Same CDS sequence) XM_025000312.1 , XM_025000311.1 , XM_025000310.1
    Accession Version XM_025000311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA CGCAGTGTCC
    CAAACGCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG
    ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856079.1
    CDS323..3961
    Translation

    Target ORF information:

    RefSeq Version XM_025000311.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA CGCAGTGTCC
    CAAACGCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG
    ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221438
    Clone ID Related Accession (Same CDS sequence) XM_025000312.1 , XM_025000311.1 , XM_025000310.1
    Accession Version XM_025000310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA CGCAGTGTCC
    CAAACGCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG
    ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856078.1
    CDS827..4465
    Translation

    Target ORF information:

    RefSeq Version XM_025000310.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGCTTTTGA AATCCTGTCA CGCAGTGTCC
    CAAACGCAAG GCGAAGGAGA TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG
    ACCAGCAACA GTTGTGTGAA ACTCCAGGAC ACAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221439
    Clone ID Related Accession (Same CDS sequence) XM_025000313.1 , XM_025000314.1
    Accession Version XM_025000313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT
    GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG
    ATGGCATCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT
    CATTATAACA TGTCTTCCTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC
    ATTGAATTTG TGAATTATAA TAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
    GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAGTTGAATC AGGGGAAATT TGAGTATAAT
    GGATCGTGTG GGTACCTTCT CAAACCAGAT TTCATGAGAC GGCCTGATCG AACATTTGAT
    CCTTTCTCTG AAACGCCTGT TGATGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA
    TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA TATGTATGGG
    CTGCCCACGG ACACTATCCG GAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT
    GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT
    TTATCACTGC CAACCATTTT CTGCAATATT GTCCTAAAAA CATATGTGCC TGATGGATTC
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA
    GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC
    ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG
    AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG
    AAAGGTATTG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    TTGAAGCATT TAAAGAAACA ACAGAAAGAG CTCAGTTCTT TAAAGAAGAA ACATGCAAAG
    GAGCACAGTA CCATGCAGAA ACTCCATTGC ACTCAAGTTG ACAAGATTGT GGCACAGTAC
    GACAAGGAGA AATTGACTCA CGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAAGGGGGA
    AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT
    CACAAATCTA AGGTCAAGGA GATTGTGGCA CAGCACACAA AGGAGTGGTC AGACATGATC
    AACACCCACA GTGCGGAGGA GCAGGAGATC CGGGACCTGC ACCTCAGCCA GCAGTGCGAG
    CTGCTCAGAA AGCTCCTGAT CAGCGCCCAC GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAAGA GATGCGAGCA CACCAAGCTA AGATTTCTAT GGAAAATAGC
    AAAGCCATCA GCCAAGATAA ATCCATCAAG AATAAAGCAG AACGGGAAAG GAGAGTCAGG
    GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGCGACT CGCCATGAAG
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AGCACCTCGA ATTCCTAGAG
    AAACAGAATG AGCAGCTTTT GAAATCCTGT CACGCAGTGT CCCAAACGCA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTTGTGTG
    AAACTCCAGG ACACAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856081.1
    CDS827..4447
    Translation

    Target ORF information:

    RefSeq Version XM_025000313.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT
    GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG
    ATGGCATCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT
    CATTATAACA TGTCTTCCTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC
    ATTGAATTTG TGAATTATAA TAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
    GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAGTTGAATC AGGGGAAATT TGAGTATAAT
    GGATCGTGTG GGTACCTTCT CAAACCAGAT TTCATGAGAC GGCCTGATCG AACATTTGAT
    CCTTTCTCTG AAACGCCTGT TGATGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA
    TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA TATGTATGGG
    CTGCCCACGG ACACTATCCG GAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT
    GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT
    TTATCACTGC CAACCATTTT CTGCAATATT GTCCTAAAAA CATATGTGCC TGATGGATTC
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA
    GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC
    ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG
    AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG
    AAAGGTATTG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    TTGAAGCATT TAAAGAAACA ACAGAAAGAG CTCAGTTCTT TAAAGAAGAA ACATGCAAAG
    GAGCACAGTA CCATGCAGAA ACTCCATTGC ACTCAAGTTG ACAAGATTGT GGCACAGTAC
    GACAAGGAGA AATTGACTCA CGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAAGGGGGA
    AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT
    CACAAATCTA AGGTCAAGGA GATTGTGGCA CAGCACACAA AGGAGTGGTC AGACATGATC
    AACACCCACA GTGCGGAGGA GCAGGAGATC CGGGACCTGC ACCTCAGCCA GCAGTGCGAG
    CTGCTCAGAA AGCTCCTGAT CAGCGCCCAC GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAAGA GATGCGAGCA CACCAAGCTA AGATTTCTAT GGAAAATAGC
    AAAGCCATCA GCCAAGATAA ATCCATCAAG AATAAAGCAG AACGGGAAAG GAGAGTCAGG
    GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGCGACT CGCCATGAAG
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AGCACCTCGA ATTCCTAGAG
    AAACAGAATG AGCAGCTTTT GAAATCCTGT CACGCAGTGT CCCAAACGCA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTTGTGTG
    AAACTCCAGG ACACAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221439
    Clone ID Related Accession (Same CDS sequence) XM_025000313.1 , XM_025000314.1
    Accession Version XM_025000314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT
    GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG
    ATGGCATCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT
    CATTATAACA TGTCTTCCTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC
    ATTGAATTTG TGAATTATAA TAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
    GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAGTTGAATC AGGGGAAATT TGAGTATAAT
    GGATCGTGTG GGTACCTTCT CAAACCAGAT TTCATGAGAC GGCCTGATCG AACATTTGAT
    CCTTTCTCTG AAACGCCTGT TGATGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA
    TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA TATGTATGGG
    CTGCCCACGG ACACTATCCG GAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT
    GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT
    TTATCACTGC CAACCATTTT CTGCAATATT GTCCTAAAAA CATATGTGCC TGATGGATTC
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA
    GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC
    ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG
    AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG
    AAAGGTATTG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    TTGAAGCATT TAAAGAAACA ACAGAAAGAG CTCAGTTCTT TAAAGAAGAA ACATGCAAAG
    GAGCACAGTA CCATGCAGAA ACTCCATTGC ACTCAAGTTG ACAAGATTGT GGCACAGTAC
    GACAAGGAGA AATTGACTCA CGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAAGGGGGA
    AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT
    CACAAATCTA AGGTCAAGGA GATTGTGGCA CAGCACACAA AGGAGTGGTC AGACATGATC
    AACACCCACA GTGCGGAGGA GCAGGAGATC CGGGACCTGC ACCTCAGCCA GCAGTGCGAG
    CTGCTCAGAA AGCTCCTGAT CAGCGCCCAC GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAAGA GATGCGAGCA CACCAAGCTA AGATTTCTAT GGAAAATAGC
    AAAGCCATCA GCCAAGATAA ATCCATCAAG AATAAAGCAG AACGGGAAAG GAGAGTCAGG
    GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGCGACT CGCCATGAAG
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AGCACCTCGA ATTCCTAGAG
    AAACAGAATG AGCAGCTTTT GAAATCCTGT CACGCAGTGT CCCAAACGCA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTTGTGTG
    AAACTCCAGG ACACAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856082.1
    CDS322..3942
    Translation

    Target ORF information:

    RefSeq Version XM_025000314.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGCTTCAGAT
    GACCTTGAAC ATGAAAACAG CAAAAAGGGC CTGGTTACCG TAGAAGATGA GCAGGCGTGG
    ATGGCATCTT ATAAATATGT GGGTGCTACC ACTAATATCC ATCCGTATTT GTCCACAATG
    ATCAACTATG CACAGCCTGT AAAGTTTCAA GGTTTCCATG TGGCTGAAGA ACGCAATATT
    CATTATAACA TGTCTTCCTT TAATGAGTCA GTCGGTCTAG GCTACTTGAA GACACACGCC
    ATTGAATTTG TGAATTATAA TAAACGGCAA ATGAGTCGCA TTTACCCCAA GGGAGGCCGA
    GTGGACTCCA GTAATTACAT GCCTCAGATT TTCTGGAACG CTGGCTGCCA GATGGTTTCG
    CTGAACTATC AAACCCCAGA TTTAGCGATG CAGTTGAATC AGGGGAAATT TGAGTATAAT
    GGATCGTGTG GGTACCTTCT CAAACCAGAT TTCATGAGAC GGCCTGATCG AACATTTGAT
    CCTTTCTCTG AAACGCCTGT TGATGGGGTT ATTGCAGCCA CTTGCTCCGT GCAGGTCATA
    TCAGGTCAGT TCTTATCAGA TAAGAAAATT GGCACATATG TAGAAGTGGA TATGTATGGG
    CTGCCCACGG ACACTATCCG GAAGGAGTTC CGCACTCGCA TGGTGATGAA CAACGGACTC
    AATCCAGTTT ACAATGAAGA ATCATTTGTG TTTCGGAAGG TGATCCTCCC CGACCTGGCT
    GTCTTGAGGA TCGCCGTGTA TGACGACAAC AACAAGCTGA TCGGCCAGAG GATTCTGCCC
    CTGGATGGCC TCCAAGCAGG CTATCGACAC ATATCCCTCC GGAATGAGGG AAATAAACCT
    TTATCACTGC CAACCATTTT CTGCAATATT GTCCTAAAAA CATATGTGCC TGATGGATTC
    GGAGATATTG TGGATGCTTT ATCAGATCCA AAGAAATTTC TTTCCATTAC GGAAAAGAGA
    GCGGATCAAA TGAGAGCTAT GGGCATTGAA ACTAGTGACA TCGCCGATGT GCCCAGTGAC
    ACTTCAAAGA ACGACAAGAA AGGGAAAGCC AACACAGCCA AAGCGAATGT GACCCCTCAG
    AGCAGCTCGG AGCTCAGGCC AACCACCACA GCTGCCCTGG GGGCTGGCCT GGAGGCCAAG
    AAAGGTATTG AACTGATCCC TCAAGTGAGG ATAGAAGATT TAAAGCAGAT GAAGGCTTAC
    TTGAAGCATT TAAAGAAACA ACAGAAAGAG CTCAGTTCTT TAAAGAAGAA ACATGCAAAG
    GAGCACAGTA CCATGCAGAA ACTCCATTGC ACTCAAGTTG ACAAGATTGT GGCACAGTAC
    GACAAGGAGA AATTGACTCA CGAGAAAATC CTAGAGAAGG CAATGAAGAA GAAAGGGGGA
    AGTAATTGTC TTGAAATGAA GAAAGAAACA GAAATTAAAA TTCAGACGCT GACATCAGAT
    CACAAATCTA AGGTCAAGGA GATTGTGGCA CAGCACACAA AGGAGTGGTC AGACATGATC
    AACACCCACA GTGCGGAGGA GCAGGAGATC CGGGACCTGC ACCTCAGCCA GCAGTGCGAG
    CTGCTCAGAA AGCTCCTGAT CAGCGCCCAC GAGCAGCAGA CCCAGCAGCT GAAACTGTCC
    CATGACAGGG AAAGCAAAGA GATGCGAGCA CACCAAGCTA AGATTTCTAT GGAAAATAGC
    AAAGCCATCA GCCAAGATAA ATCCATCAAG AATAAAGCAG AACGGGAAAG GAGAGTCAGG
    GAGTTAAACA GCAGCAACAC TAAAAAGTTT CTGGAAGAAA GAAAGCGACT CGCCATGAAG
    CAGTCCAAAG AAATGGATCA GTTGAAAAAA GTCCAGCTTG AGCACCTCGA ATTCCTAGAG
    AAACAGAATG AGCAGCTTTT GAAATCCTGT CACGCAGTGT CCCAAACGCA AGGCGAAGGA
    GATGCAGCAG ATGGTGAAAT TGGAAGCCGA GATGGACCGC AGACCAGCAA CAGTTGTGTG
    AAACTCCAGG ACACAAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221440
    Clone ID Related Accession (Same CDS sequence) XM_025000315.1
    Accession Version XM_025000315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCATC TTATAAATAT
    GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA TGCACAGCCT
    GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTATAA CATGTCTTCC
    TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATTGAATT TGTGAATTAT
    AATAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGCC GAGTGGACTC CAGTAATTAC
    ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA
    GATTTAGCGA TGCAGTTGAA TCAGGGGAAA TTTGAGTATA ATGGATCGTG TGGGTACCTT
    CTCAAACCAG ATTTCATGAG ACGGCCTGAT CGAACATTTG ATCCTTTCTC TGAAACGCCT
    GTTGATGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA
    GATAAGAAAA TTGGCACATA TGTAGAAGTG GATATGTATG GGCTGCCCAC GGACACTATC
    CGGAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGAC TCAATCCAGT TTACAATGAA
    GAATCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG
    TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTCCAAGCA
    GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCACT GCCAACCATT
    TTCTGCAATA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT
    TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT
    ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG
    AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG
    CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT TGAACTGATC
    CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA
    CAACAGAAAG AGCTCAGTTC TTTAAAGAAG AAACATGCAA AGGAGCACAG TACCATGCAG
    AAACTCCATT GCACTCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT
    CACGAGAAAA TCCTAGAGAA GGCAATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG
    AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG
    GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG
    GAGCAGGAGA TCCGGGACCT GCACCTCAGC CAGCAGTGCG AGCTGCTCAG AAAGCTCCTG
    ATCAGCGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA
    GAGATGCGAG CACACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT
    AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA CAGCAGCAAC
    ACTAAAAAGT TTCTGGAAGA AAGAAAGCGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT
    CAGTTGAAAA AAGTCCAGCT TGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGCTT
    TTGAAATCCT GTCACGCAGT GTCCCAAACG CAAGGCGAAG GAGATGCAGC AGATGGTGAA
    ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG TGAAACTCCA GGACACAAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856083.1
    CDS827..4429
    Translation

    Target ORF information:

    RefSeq Version XM_025000315.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCATC TTATAAATAT
    GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA TGCACAGCCT
    GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTATAA CATGTCTTCC
    TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATTGAATT TGTGAATTAT
    AATAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGCC GAGTGGACTC CAGTAATTAC
    ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA
    GATTTAGCGA TGCAGTTGAA TCAGGGGAAA TTTGAGTATA ATGGATCGTG TGGGTACCTT
    CTCAAACCAG ATTTCATGAG ACGGCCTGAT CGAACATTTG ATCCTTTCTC TGAAACGCCT
    GTTGATGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA
    GATAAGAAAA TTGGCACATA TGTAGAAGTG GATATGTATG GGCTGCCCAC GGACACTATC
    CGGAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGAC TCAATCCAGT TTACAATGAA
    GAATCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG
    TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTCCAAGCA
    GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCACT GCCAACCATT
    TTCTGCAATA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT
    TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT
    ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG
    AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG
    CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT TGAACTGATC
    CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA
    CAACAGAAAG AGCTCAGTTC TTTAAAGAAG AAACATGCAA AGGAGCACAG TACCATGCAG
    AAACTCCATT GCACTCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT
    CACGAGAAAA TCCTAGAGAA GGCAATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG
    AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG
    GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG
    GAGCAGGAGA TCCGGGACCT GCACCTCAGC CAGCAGTGCG AGCTGCTCAG AAAGCTCCTG
    ATCAGCGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA
    GAGATGCGAG CACACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT
    AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA CAGCAGCAAC
    ACTAAAAAGT TTCTGGAAGA AAGAAAGCGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT
    CAGTTGAAAA AAGTCCAGCT TGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGCTT
    TTGAAATCCT GTCACGCAGT GTCCCAAACG CAAGGCGAAG GAGATGCAGC AGATGGTGAA
    ATTGGAAGCC GAGATGGACC GCAGACCAGC AACAGTTGTG TGAAACTCCA GGACACAAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221441
    Clone ID Related Accession (Same CDS sequence) XM_025000316.1 , NM_001166510.2
    Accession Version XM_025000316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856084.1
    CDS322..3906
    Translation

    Target ORF information:

    RefSeq Version XM_025000316.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGACATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221442
    Clone ID Related Accession (Same CDS sequence) XM_025000317.1
    Accession Version XM_025000317.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCATAT TACTAGAACC
    TTTGCATCGG GGAAAACAGA AAAGGTGATC TTTCAAGCAC TCAAGGAGCT AGGTCTTCCC
    AGTGGAAAGA ATGATGAAAT TGAGCCTGCC GCATTTACTT ATGAAAAGTT CTATGAACTG
    ACACAAAAGA TTTGTCCTCG GACAGATATA GAGGATCTTT TCAAAAAAAT CAATGGAGAC
    AAAACTGATT ATTTAACGGT AGACCAATTA GTGAGCTTTC TAAATGAACA TCAACGAGAT
    CCTCGACTGA ATGAAATTTT ATTCCCATTT TATGATGCTA AAAGGGCAAT GCAGATCATT
    GAGATGTATG AGCCCGATGA AGATTTGAAG AAACAAGGCC TCATATCAAG TGATGGGTTT
    TGCAGATACC TGATGTCAGA TGAAAACGCC CCCGTCTTCC TAGATCGTCT AGAGCTGTAC
    CAAGAAATGG ACCACCCTCT GGCTCACTAC TTCATCAGCT CTTCCCACAA CACCTATCTC
    ACTGGCAGAC AGTTTGGTGG GAAATCTTCA GTAGAAATGT ACAGACAGGT CCTCCTGGCT
    GGTTGCAGAT GTGTTGAACT TGACTGTTGG GATGGAAAAG GTGAGGACCA GGAACCGATA
    ATAACTCACG GGAAAGCAAT GTGCACAGAC ATCCTTTTTA AGGATGTTAT TCAAGCCATC
    AAGGAAACTG CCTTTGTCAC ATCGGAATAT CCTGTAATTC TCTCCTTTGA AAATCACTGC
    AGCAAATATC AACAGTACAA GATGTCCAAA TACTGTGAAG ATCTATTTGG AGATCTCCTG
    TTGAAACAAG CACTTGAATC ACATCCACTG GAACCAGGCA GACCCTTGCC ATCCCCAAAT
    GACCTCAAAA GAAAAATACT CATCAAAAAC AAACGACTGA AACCCGAGGT TGAAAAAAAA
    CAGCTTGAAG CTTTGAAAAG CATGATGGAA GCCGGAGAAT CCGCTGCCCC GGTGAACATC
    TTAGAGGATG ATAATGAAGA GGAAATAGAA AGTGCTGAGC AAGAGGAGGA AGCCCACCCT
    GAATACAAAT ATGGAAGTGA ACTTTCTGCA GATGACTTGG GTCACAAGGA AGCTATTGCC
    AATAGTGTCA AGAAGGCTTC AGATGACCTT GAACATGAAA ACAGCAAAAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856085.1
    CDS829..4413
    Translation

    Target ORF information:

    RefSeq Version XM_025000317.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCATAT TACTAGAACC
    TTTGCATCGG GGAAAACAGA AAAGGTGATC TTTCAAGCAC TCAAGGAGCT AGGTCTTCCC
    AGTGGAAAGA ATGATGAAAT TGAGCCTGCC GCATTTACTT ATGAAAAGTT CTATGAACTG
    ACACAAAAGA TTTGTCCTCG GACAGATATA GAGGATCTTT TCAAAAAAAT CAATGGAGAC
    AAAACTGATT ATTTAACGGT AGACCAATTA GTGAGCTTTC TAAATGAACA TCAACGAGAT
    CCTCGACTGA ATGAAATTTT ATTCCCATTT TATGATGCTA AAAGGGCAAT GCAGATCATT
    GAGATGTATG AGCCCGATGA AGATTTGAAG AAACAAGGCC TCATATCAAG TGATGGGTTT
    TGCAGATACC TGATGTCAGA TGAAAACGCC CCCGTCTTCC TAGATCGTCT AGAGCTGTAC
    CAAGAAATGG ACCACCCTCT GGCTCACTAC TTCATCAGCT CTTCCCACAA CACCTATCTC
    ACTGGCAGAC AGTTTGGTGG GAAATCTTCA GTAGAAATGT ACAGACAGGT CCTCCTGGCT
    GGTTGCAGAT GTGTTGAACT TGACTGTTGG GATGGAAAAG GTGAGGACCA GGAACCGATA
    ATAACTCACG GGAAAGCAAT GTGCACAGAC ATCCTTTTTA AGGATGTTAT TCAAGCCATC
    AAGGAAACTG CCTTTGTCAC ATCGGAATAT CCTGTAATTC TCTCCTTTGA AAATCACTGC
    AGCAAATATC AACAGTACAA GATGTCCAAA TACTGTGAAG ATCTATTTGG AGATCTCCTG
    TTGAAACAAG CACTTGAATC ACATCCACTG GAACCAGGCA GACCCTTGCC ATCCCCAAAT
    GACCTCAAAA GAAAAATACT CATCAAAAAC AAACGACTGA AACCCGAGGT TGAAAAAAAA
    CAGCTTGAAG CTTTGAAAAG CATGATGGAA GCCGGAGAAT CCGCTGCCCC GGTGAACATC
    TTAGAGGATG ATAATGAAGA GGAAATAGAA AGTGCTGAGC AAGAGGAGGA AGCCCACCCT
    GAATACAAAT ATGGAAGTGA ACTTTCTGCA GATGACTTGG GTCACAAGGA AGCTATTGCC
    AATAGTGTCA AGAAGGCTTC AGATGACCTT GAACATGAAA ACAGCAAAAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221443
    Clone ID Related Accession (Same CDS sequence) XM_025000318.1
    Accession Version XM_025000318.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGGCGAAGG AGATGCAGCA GATGGTGAAA
    TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856086.1
    CDS830..4411
    Translation

    Target ORF information:

    RefSeq Version XM_025000318.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG CTTCAGATGA CCTTGAACAT GAAAACAGCA AAAAGGGCCT GGTTACCGTA
    GAAGATGAGC AGGCGTGGAT GGCATCTTAT AAATATGTGG GTGCTACCAC TAATATCCAT
    CCGTATTTGT CCACAATGAT CAACTATGCA CAGCCTGTAA AGTTTCAAGG TTTCCATGTG
    GCTGAAGAAC GCAATATTCA TTATAACATG TCTTCCTTTA ATGAGTCAGT CGGTCTAGGC
    TACTTGAAGA CACACGCCAT TGAATTTGTG AATTATAATA AACGGCAAAT GAGTCGCATT
    TACCCCAAGG GAGGCCGAGT GGACTCCAGT AATTACATGC CTCAGATTTT CTGGAACGCT
    GGCTGCCAGA TGGTTTCGCT GAACTATCAA ACCCCAGATT TAGCGATGCA GTTGAATCAG
    GGGAAATTTG AGTATAATGG ATCGTGTGGG TACCTTCTCA AACCAGATTT CATGAGACGG
    CCTGATCGAA CATTTGATCC TTTCTCTGAA ACGCCTGTTG ATGGGGTTAT TGCAGCCACT
    TGCTCCGTGC AGGTCATATC AGGTCAGTTC TTATCAGATA AGAAAATTGG CACATATGTA
    GAAGTGGATA TGTATGGGCT GCCCACGGAC ACTATCCGGA AGGAGTTCCG CACTCGCATG
    GTGATGAACA ACGGACTCAA TCCAGTTTAC AATGAAGAAT CATTTGTGTT TCGGAAGGTG
    ATCCTCCCCG ACCTGGCTGT CTTGAGGATC GCCGTGTATG ACGACAACAA CAAGCTGATC
    GGCCAGAGGA TTCTGCCCCT GGATGGCCTC CAAGCAGGCT ATCGACACAT ATCCCTCCGG
    AATGAGGGAA ATAAACCTTT ATCACTGCCA ACCATTTTCT GCAATATTGT CCTAAAAACA
    TATGTGCCTG ATGGATTCGG AGATATTGTG GATGCTTTAT CAGATCCAAA GAAATTTCTT
    TCCATTACGG AAAAGAGAGC GGATCAAATG AGAGCTATGG GCATTGAAAC TAGTGACATC
    GCCGATGTGC CCAGTGACAC TTCAAAGAAC GACAAGAAAG GGAAAGCCAA CACAGCCAAA
    GCGAATGTGA CCCCTCAGAG CAGCTCGGAG CTCAGGCCAA CCACCACAGC TGCCCTGGGG
    GCTGGCCTGG AGGCCAAGAA AGGTATTGAA CTGATCCCTC AAGTGAGGAT AGAAGATTTA
    AAGCAGATGA AGGCTTACTT GAAGCATTTA AAGAAACAAC AGAAAGAGCT CAGTTCTTTA
    AAGAAGAAAC ATGCAAAGGA GCACAGTACC ATGCAGAAAC TCCATTGCAC TCAAGTTGAC
    AAGATTGTGG CACAGTACGA CAAGGAGAAA TTGACTCACG AGAAAATCCT AGAGAAGGCA
    ATGAAGAAGA AAGGGGGAAG TAATTGTCTT GAAATGAAGA AAGAAACAGA AATTAAAATT
    CAGACGCTGA CATCAGATCA CAAATCTAAG GTCAAGGAGA TTGTGGCACA GCACACAAAG
    GAGTGGTCAG ACATGATCAA CACCCACAGT GCGGAGGAGC AGGAGATCCG GGACCTGCAC
    CTCAGCCAGC AGTGCGAGCT GCTCAGAAAG CTCCTGATCA GCGCCCACGA GCAGCAGACC
    CAGCAGCTGA AACTGTCCCA TGACAGGGAA AGCAAAGAGA TGCGAGCACA CCAAGCTAAG
    ATTTCTATGG AAAATAGCAA AGCCATCAGC CAAGATAAAT CCATCAAGAA TAAAGCAGAA
    CGGGAAAGGA GAGTCAGGGA GTTAAACAGC AGCAACACTA AAAAGTTTCT GGAAGAAAGA
    AAGCGACTCG CCATGAAGCA GTCCAAAGAA ATGGATCAGT TGAAAAAAGT CCAGCTTGAG
    CACCTCGAAT TCCTAGAGAA ACAGAATGAG CAGGCGAAGG AGATGCAGCA GATGGTGAAA
    TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221444
    Clone ID Related Accession (Same CDS sequence) XM_025000319.1
    Accession Version XM_025000319.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCATC TTATAAATAT
    GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA TGCACAGCCT
    GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTATAA CATGTCTTCC
    TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATTGAATT TGTGAATTAT
    AATAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGCC GAGTGGACTC CAGTAATTAC
    ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA
    GATTTAGCGA TGCAGTTGAA TCAGGGGAAA TTTGAGTATA ATGGATCGTG TGGGTACCTT
    CTCAAACCAG ATTTCATGAG ACGGCCTGAT CGAACATTTG ATCCTTTCTC TGAAACGCCT
    GTTGATGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA
    GATAAGAAAA TTGGCACATA TGTAGAAGTG GATATGTATG GGCTGCCCAC GGACACTATC
    CGGAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGAC TCAATCCAGT TTACAATGAA
    GAATCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG
    TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTCCAAGCA
    GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCACT GCCAACCATT
    TTCTGCAATA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT
    TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT
    ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG
    AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG
    CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT TGAACTGATC
    CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA
    CAACAGAAAG AGCTCAGTTC TTTAAAGAAG AAACATGCAA AGGAGCACAG TACCATGCAG
    AAACTCCATT GCACTCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT
    CACGAGAAAA TCCTAGAGAA GGCAATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG
    AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG
    GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG
    GAGCAGGAGA TCCGGGACCT GCACCTCAGC CAGCAGTGCG AGCTGCTCAG AAAGCTCCTG
    ATCAGCGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA
    GAGATGCGAG CACACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT
    AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA CAGCAGCAAC
    ACTAAAAAGT TTCTGGAAGA AAGAAAGCGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT
    CAGTTGAAAA AAGTCCAGCT TGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGGCG
    AAGGAGATGC AGCAGATGGT GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856087.1
    CDS830..4375
    Translation

    Target ORF information:

    RefSeq Version XM_025000319.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGTCTTGA CTCTAATCAT GGCCAAACCT TATGAATTTA ATTGGCAGAA GGAAGTTCCT 
    TCCTTTTTGC AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCCTT TGTGTTTGAA
    CCCAACTGCC TCTTCAAAGT AGATGAATTT GGCTTCTTTC TGACCTGGAG AAGTGAAGGC
    AAGGAAGGCC AAGTGCTAGA ATGCTCCCTC ATCAACAGTG TTCGGCTGGG AGCCACACCG
    AAGGATCCCA AAATTTTGGC TGCTCTTGAA GCTCTTGGCA AATCAGAAAA TGATCTGGAA
    GGGCGGATAG TTTGCGTCTG CAGTGGTGCA GATCTGGTGA ACATCAGTTT CACGTACATG
    GTGGCTGAAA ATCCAGAAAT AACTAAGCAA TGGGTCGAAG GCCTGAGATC CATCATTCAC
    AACTTCAGGG CCAACAACGT CAGCCCGATG ACGTGCCTCA AGAAGCACTG GATGAAATTG
    GCATTTATGA CCAACACAAA TGGGAAAATT CCAGTTAGGA GTATTACTAG AACCTTTGCA
    TCGGGGAAAA CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGCTAGGTCT TCCCAGTGGA
    AAGAATGATG AAATTGAGCC TGCCGCATTT ACTTATGAAA AGTTCTATGA ACTGACACAA
    AAGATTTGTC CTCGGACAGA TATAGAGGAT CTTTTCAAAA AAATCAATGG AGACAAAACT
    GATTATTTAA CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA
    CTGAATGAAA TTTTATTCCC ATTTTATGAT GCTAAAAGGG CAATGCAGAT CATTGAGATG
    TATGAGCCCG ATGAAGATTT GAAGAAACAA GGCCTCATAT CAAGTGATGG GTTTTGCAGA
    TACCTGATGT CAGATGAAAA CGCCCCCGTC TTCCTAGATC GTCTAGAGCT GTACCAAGAA
    ATGGACCACC CTCTGGCTCA CTACTTCATC AGCTCTTCCC ACAACACCTA TCTCACTGGC
    AGACAGTTTG GTGGGAAATC TTCAGTAGAA ATGTACAGAC AGGTCCTCCT GGCTGGTTGC
    AGATGTGTTG AACTTGACTG TTGGGATGGA AAAGGTGAGG ACCAGGAACC GATAATAACT
    CACGGGAAAG CAATGTGCAC AGACATCCTT TTTAAGGATG TTATTCAAGC CATCAAGGAA
    ACTGCCTTTG TCACATCGGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA
    TATCAACAGT ACAAGATGTC CAAATACTGT GAAGATCTAT TTGGAGATCT CCTGTTGAAA
    CAAGCACTTG AATCACATCC ACTGGAACCA GGCAGACCCT TGCCATCCCC AAATGACCTC
    AAAAGAAAAA TACTCATCAA AAACAAACGA CTGAAACCCG AGGTTGAAAA AAAACAGCTT
    GAAGCTTTGA AAAGCATGAT GGAAGCCGGA GAATCCGCTG CCCCGGTGAA CATCTTAGAG
    GATGATAATG AAGAGGAAAT AGAAAGTGCT GAGCAAGAGG AGGAAGCCCA CCCTGAATAC
    AAATATGGAA GTGAACTTTC TGCAGATGAC TTGGGTCACA AGGAAGCTAT TGCCAATAGT
    GTCAAGAAGG GCCTGGTTAC CGTAGAAGAT GAGCAGGCGT GGATGGCATC TTATAAATAT
    GTGGGTGCTA CCACTAATAT CCATCCGTAT TTGTCCACAA TGATCAACTA TGCACAGCCT
    GTAAAGTTTC AAGGTTTCCA TGTGGCTGAA GAACGCAATA TTCATTATAA CATGTCTTCC
    TTTAATGAGT CAGTCGGTCT AGGCTACTTG AAGACACACG CCATTGAATT TGTGAATTAT
    AATAAACGGC AAATGAGTCG CATTTACCCC AAGGGAGGCC GAGTGGACTC CAGTAATTAC
    ATGCCTCAGA TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CGCTGAACTA TCAAACCCCA
    GATTTAGCGA TGCAGTTGAA TCAGGGGAAA TTTGAGTATA ATGGATCGTG TGGGTACCTT
    CTCAAACCAG ATTTCATGAG ACGGCCTGAT CGAACATTTG ATCCTTTCTC TGAAACGCCT
    GTTGATGGGG TTATTGCAGC CACTTGCTCC GTGCAGGTCA TATCAGGTCA GTTCTTATCA
    GATAAGAAAA TTGGCACATA TGTAGAAGTG GATATGTATG GGCTGCCCAC GGACACTATC
    CGGAAGGAGT TCCGCACTCG CATGGTGATG AACAACGGAC TCAATCCAGT TTACAATGAA
    GAATCATTTG TGTTTCGGAA GGTGATCCTC CCCGACCTGG CTGTCTTGAG GATCGCCGTG
    TATGACGACA ACAACAAGCT GATCGGCCAG AGGATTCTGC CCCTGGATGG CCTCCAAGCA
    GGCTATCGAC ACATATCCCT CCGGAATGAG GGAAATAAAC CTTTATCACT GCCAACCATT
    TTCTGCAATA TTGTCCTAAA AACATATGTG CCTGATGGAT TCGGAGATAT TGTGGATGCT
    TTATCAGATC CAAAGAAATT TCTTTCCATT ACGGAAAAGA GAGCGGATCA AATGAGAGCT
    ATGGGCATTG AAACTAGTGA CATCGCCGAT GTGCCCAGTG ACACTTCAAA GAACGACAAG
    AAAGGGAAAG CCAACACAGC CAAAGCGAAT GTGACCCCTC AGAGCAGCTC GGAGCTCAGG
    CCAACCACCA CAGCTGCCCT GGGGGCTGGC CTGGAGGCCA AGAAAGGTAT TGAACTGATC
    CCTCAAGTGA GGATAGAAGA TTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA
    CAACAGAAAG AGCTCAGTTC TTTAAAGAAG AAACATGCAA AGGAGCACAG TACCATGCAG
    AAACTCCATT GCACTCAAGT TGACAAGATT GTGGCACAGT ACGACAAGGA GAAATTGACT
    CACGAGAAAA TCCTAGAGAA GGCAATGAAG AAGAAAGGGG GAAGTAATTG TCTTGAAATG
    AAGAAAGAAA CAGAAATTAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAG
    GAGATTGTGG CACAGCACAC AAAGGAGTGG TCAGACATGA TCAACACCCA CAGTGCGGAG
    GAGCAGGAGA TCCGGGACCT GCACCTCAGC CAGCAGTGCG AGCTGCTCAG AAAGCTCCTG
    ATCAGCGCCC ACGAGCAGCA GACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAA
    GAGATGCGAG CACACCAAGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT
    AAATCCATCA AGAATAAAGC AGAACGGGAA AGGAGAGTCA GGGAGTTAAA CAGCAGCAAC
    ACTAAAAAGT TTCTGGAAGA AAGAAAGCGA CTCGCCATGA AGCAGTCCAA AGAAATGGAT
    CAGTTGAAAA AAGTCCAGCT TGAGCACCTC GAATTCCTAG AGAAACAGAA TGAGCAGGCG
    AAGGAGATGC AGCAGATGGT GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTT
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221445
    Clone ID Related Accession (Same CDS sequence) XM_025000320.1
    Accession Version XM_025000320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACGTGCC TCAAGAAGCA CTGGATGAAA TTGGCATTTA TGACCAACAC AAATGGGAAA 
    ATTCCAGTTA GGAGTATTAC TAGAACCTTT GCATCGGGGA AAACAGAAAA GGTGATCTTT
    CAAGCACTCA AGGAGCTAGG TCTTCCCAGT GGAAAGAATG ATGAAATTGA GCCTGCCGCA
    TTTACTTATG AAAAGTTCTA TGAACTGACA CAAAAGATTT GTCCTCGGAC AGATATAGAG
    GATCTTTTCA AAAAAATCAA TGGAGACAAA ACTGATTATT TAACGGTAGA CCAATTAGTG
    AGCTTTCTAA ATGAACATCA ACGAGATCCT CGACTGAATG AAATTTTATT CCCATTTTAT
    GATGCTAAAA GGGCAATGCA GATCATTGAG ATGTATGAGC CCGATGAAGA TTTGAAGAAA
    CAAGGCCTCA TATCAAGTGA TGGGTTTTGC AGATACCTGA TGTCAGATGA AAACGCCCCC
    GTCTTCCTAG ATCGTCTAGA GCTGTACCAA GAAATGGACC ACCCTCTGGC TCACTACTTC
    ATCAGCTCTT CCCACAACAC CTATCTCACT GGCAGACAGT TTGGTGGGAA ATCTTCAGTA
    GAAATGTACA GACAGGTCCT CCTGGCTGGT TGCAGATGTG TTGAACTTGA CTGTTGGGAT
    GGAAAAGGTG AGGACCAGGA ACCGATAATA ACTCACGGGA AAGCAATGTG CACAGACATC
    CTTTTTAAGG ATGTTATTCA AGCCATCAAG GAAACTGCCT TTGTCACATC GGAATATCCT
    GTAATTCTCT CCTTTGAAAA TCACTGCAGC AAATATCAAC AGTACAAGAT GTCCAAATAC
    TGTGAAGATC TATTTGGAGA TCTCCTGTTG AAACAAGCAC TTGAATCACA TCCACTGGAA
    CCAGGCAGAC CCTTGCCATC CCCAAATGAC CTCAAAAGAA AAATACTCAT CAAAAACAAA
    CGACTGAAAC CCGAGGTTGA AAAAAAACAG CTTGAAGCTT TGAAAAGCAT GATGGAAGCC
    GGAGAATCCG CTGCCCCGGT GAACATCTTA GAGGATGATA ATGAAGAGGA AATAGAAAGT
    GCTGAGCAAG AGGAGGAAGC CCACCCTGAA TACAAATATG GAAGTGAACT TTCTGCAGAT
    GACTTGGGTC ACAAGGAAGC TATTGCCAAT AGTGTCAAGA AGGCTTCAGA TGACCTTGAA
    CATGAAAACA GCAAAAAGGG CCTGGTTACC GTAGAAGATG AGCAGGCGTG GATGGCATCT
    TATAAATATG TGGGTGCTAC CACTAATATC CATCCGTATT TGTCCACAAT GATCAACTAT
    GCACAGCCTG TAAAGTTTCA AGGTTTCCAT GTGGCTGAAG AACGCAATAT TCATTATAAC
    ATGTCTTCCT TTAATGAGTC AGTCGGTCTA GGCTACTTGA AGACACACGC CATTGAATTT
    GTGAATTATA ATAAACGGCA AATGAGTCGC ATTTACCCCA AGGGAGGCCG AGTGGACTCC
    AGTAATTACA TGCCTCAGAT TTTCTGGAAC GCTGGCTGCC AGATGGTTTC GCTGAACTAT
    CAAACCCCAG ATTTAGCGAT GCAGTTGAAT CAGGGGAAAT TTGAGTATAA TGGATCGTGT
    GGGTACCTTC TCAAACCAGA TTTCATGAGA CGGCCTGATC GAACATTTGA TCCTTTCTCT
    GAAACGCCTG TTGATGGGGT TATTGCAGCC ACTTGCTCCG TGCAGGTCAT ATCAGGTCAG
    TTCTTATCAG ATAAGAAAAT TGGCACATAT GTAGAAGTGG ATATGTATGG GCTGCCCACG
    GACACTATCC GGAAGGAGTT CCGCACTCGC ATGGTGATGA ACAACGGACT CAATCCAGTT
    TACAATGAAG AATCATTTGT GTTTCGGAAG GTGATCCTCC CCGACCTGGC TGTCTTGAGG
    ATCGCCGTGT ATGACGACAA CAACAAGCTG ATCGGCCAGA GGATTCTGCC CCTGGATGGC
    CTCCAAGCAG GCTATCGACA CATATCCCTC CGGAATGAGG GAAATAAACC TTTATCACTG
    CCAACCATTT TCTGCAATAT TGTCCTAAAA ACATATGTGC CTGATGGATT CGGAGATATT
    GTGGATGCTT TATCAGATCC AAAGAAATTT CTTTCCATTA CGGAAAAGAG AGCGGATCAA
    ATGAGAGCTA TGGGCATTGA AACTAGTGAC ATCGCCGATG TGCCCAGTGA CACTTCAAAG
    AACGACAAGA AAGGGAAAGC CAACACAGCC AAAGCGAATG TGACCCCTCA GAGCAGCTCG
    GAGCTCAGGC CAACCACCAC AGCTGCCCTG GGGGCTGGCC TGGAGGCCAA GAAAGGTATT
    GAACTGATCC CTCAAGTGAG GATAGAAGAT TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT
    TTAAAGAAAC AACAGAAAGA GCTCAGTTCT TTAAAGAAGA AACATGCAAA GGAGCACAGT
    ACCATGCAGA AACTCCATTG CACTCAAGTT GACAAGATTG TGGCACAGTA CGACAAGGAG
    AAATTGACTC ACGAGAAAAT CCTAGAGAAG GCAATGAAGA AGAAAGGGGG AAGTAATTGT
    CTTGAAATGA AGAAAGAAAC AGAAATTAAA ATTCAGACGC TGACATCAGA TCACAAATCT
    AAGGTCAAGG AGATTGTGGC ACAGCACACA AAGGAGTGGT CAGACATGAT CAACACCCAC
    AGTGCGGAGG AGCAGGAGAT CCGGGACCTG CACCTCAGCC AGCAGTGCGA GCTGCTCAGA
    AAGCTCCTGA TCAGCGCCCA CGAGCAGCAG ACCCAGCAGC TGAAACTGTC CCATGACAGG
    GAAAGCAAAG AGATGCGAGC ACACCAAGCT AAGATTTCTA TGGAAAATAG CAAAGCCATC
    AGCCAAGATA AATCCATCAA GAATAAAGCA GAACGGGAAA GGAGAGTCAG GGAGTTAAAC
    AGCAGCAACA CTAAAAAGTT TCTGGAAGAA AGAAAGCGAC TCGCCATGAA GCAGTCCAAA
    GAAATGGATC AGTTGAAAAA AGTCCAGCTT GAGCACCTCG AATTCCTAGA GAAACAGAAT
    GAGCAGCTTT TGAAATCCTG TCACGCAGTG TCCCAAACGC AAGGCGAAGG AGATGCAGCA
    GATGGTGAAA TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GAAACTCCAG
    GACACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024856088.1
    CDS591..3782
    Translation

    Target ORF information:

    RefSeq Version XM_025000320.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C beta 4 (PLCB4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGACGTGCC TCAAGAAGCA CTGGATGAAA TTGGCATTTA TGACCAACAC AAATGGGAAA 
    ATTCCAGTTA GGAGTATTAC TAGAACCTTT GCATCGGGGA AAACAGAAAA GGTGATCTTT
    CAAGCACTCA AGGAGCTAGG TCTTCCCAGT GGAAAGAATG ATGAAATTGA GCCTGCCGCA
    TTTACTTATG AAAAGTTCTA TGAACTGACA CAAAAGATTT GTCCTCGGAC AGATATAGAG
    GATCTTTTCA AAAAAATCAA TGGAGACAAA ACTGATTATT TAACGGTAGA CCAATTAGTG
    AGCTTTCTAA ATGAACATCA ACGAGATCCT CGACTGAATG AAATTTTATT CCCATTTTAT
    GATGCTAAAA GGGCAATGCA GATCATTGAG ATGTATGAGC CCGATGAAGA TTTGAAGAAA
    CAAGGCCTCA TATCAAGTGA TGGGTTTTGC AGATACCTGA TGTCAGATGA AAACGCCCCC
    GTCTTCCTAG ATCGTCTAGA GCTGTACCAA GAAATGGACC ACCCTCTGGC TCACTACTTC
    ATCAGCTCTT CCCACAACAC CTATCTCACT GGCAGACAGT TTGGTGGGAA ATCTTCAGTA
    GAAATGTACA GACAGGTCCT CCTGGCTGGT TGCAGATGTG TTGAACTTGA CTGTTGGGAT
    GGAAAAGGTG AGGACCAGGA ACCGATAATA ACTCACGGGA AAGCAATGTG CACAGACATC
    CTTTTTAAGG ATGTTATTCA AGCCATCAAG GAAACTGCCT TTGTCACATC GGAATATCCT
    GTAATTCTCT CCTTTGAAAA TCACTGCAGC AAATATCAAC AGTACAAGAT GTCCAAATAC
    TGTGAAGATC TATTTGGAGA TCTCCTGTTG AAACAAGCAC TTGAATCACA TCCACTGGAA
    CCAGGCAGAC CCTTGCCATC CCCAAATGAC CTCAAAAGAA AAATACTCAT CAAAAACAAA
    CGACTGAAAC CCGAGGTTGA AAAAAAACAG CTTGAAGCTT TGAAAAGCAT GATGGAAGCC
    GGAGAATCCG CTGCCCCGGT GAACATCTTA GAGGATGATA ATGAAGAGGA AATAGAAAGT
    GCTGAGCAAG AGGAGGAAGC CCACCCTGAA TACAAATATG GAAGTGAACT TTCTGCAGAT
    GACTTGGGTC ACAAGGAAGC TATTGCCAAT AGTGTCAAGA AGGCTTCAGA TGACCTTGAA
    CATGAAAACA GCAAAAAGGG CCTGGTTACC GTAGAAGATG AGCAGGCGTG GATGGCATCT
    TATAAATATG TGGGTGCTAC CACTAATATC CATCCGTATT TGTCCACAAT GATCAACTAT
    GCACAGCCTG TAAAGTTTCA AGGTTTCCAT GTGGCTGAAG AACGCAATAT TCATTATAAC
    ATGTCTTCCT TTAATGAGTC AGTCGGTCTA GGCTACTTGA AGACACACGC CATTGAATTT
    GTGAATTATA ATAAACGGCA AATGAGTCGC ATTTACCCCA AGGGAGGCCG AGTGGACTCC
    AGTAATTACA TGCCTCAGAT TTTCTGGAAC GCTGGCTGCC AGATGGTTTC GCTGAACTAT
    CAAACCCCAG ATTTAGCGAT GCAGTTGAAT CAGGGGAAAT TTGAGTATAA TGGATCGTGT
    GGGTACCTTC TCAAACCAGA TTTCATGAGA CGGCCTGATC GAACATTTGA TCCTTTCTCT
    GAAACGCCTG TTGATGGGGT TATTGCAGCC ACTTGCTCCG TGCAGGTCAT ATCAGGTCAG
    TTCTTATCAG ATAAGAAAAT TGGCACATAT GTAGAAGTGG ATATGTATGG GCTGCCCACG
    GACACTATCC GGAAGGAGTT CCGCACTCGC ATGGTGATGA ACAACGGACT CAATCCAGTT
    TACAATGAAG AATCATTTGT GTTTCGGAAG GTGATCCTCC CCGACCTGGC TGTCTTGAGG
    ATCGCCGTGT ATGACGACAA CAACAAGCTG ATCGGCCAGA GGATTCTGCC CCTGGATGGC
    CTCCAAGCAG GCTATCGACA CATATCCCTC CGGAATGAGG GAAATAAACC TTTATCACTG
    CCAACCATTT TCTGCAATAT TGTCCTAAAA ACATATGTGC CTGATGGATT CGGAGATATT
    GTGGATGCTT TATCAGATCC AAAGAAATTT CTTTCCATTA CGGAAAAGAG AGCGGATCAA
    ATGAGAGCTA TGGGCATTGA AACTAGTGAC ATCGCCGATG TGCCCAGTGA CACTTCAAAG
    AACGACAAGA AAGGGAAAGC CAACACAGCC AAAGCGAATG TGACCCCTCA GAGCAGCTCG
    GAGCTCAGGC CAACCACCAC AGCTGCCCTG GGGGCTGGCC TGGAGGCCAA GAAAGGTATT
    GAACTGATCC CTCAAGTGAG GATAGAAGAT TTAAAGCAGA TGAAGGCTTA CTTGAAGCAT
    TTAAAGAAAC AACAGAAAGA GCTCAGTTCT TTAAAGAAGA AACATGCAAA GGAGCACAGT
    ACCATGCAGA AACTCCATTG CACTCAAGTT GACAAGATTG TGGCACAGTA CGACAAGGAG
    AAATTGACTC ACGAGAAAAT CCTAGAGAAG GCAATGAAGA AGAAAGGGGG AAGTAATTGT
    CTTGAAATGA AGAAAGAAAC AGAAATTAAA ATTCAGACGC TGACATCAGA TCACAAATCT
    AAGGTCAAGG AGATTGTGGC ACAGCACACA AAGGAGTGGT CAGACATGAT CAACACCCAC
    AGTGCGGAGG AGCAGGAGAT CCGGGACCTG CACCTCAGCC AGCAGTGCGA GCTGCTCAGA
    AAGCTCCTGA TCAGCGCCCA CGAGCAGCAG ACCCAGCAGC TGAAACTGTC CCATGACAGG
    GAAAGCAAAG AGATGCGAGC ACACCAAGCT AAGATTTCTA TGGAAAATAG CAAAGCCATC
    AGCCAAGATA AATCCATCAA GAATAAAGCA GAACGGGAAA GGAGAGTCAG GGAGTTAAAC
    AGCAGCAACA CTAAAAAGTT TCTGGAAGAA AGAAAGCGAC TCGCCATGAA GCAGTCCAAA
    GAAATGGATC AGTTGAAAAA AGTCCAGCTT GAGCACCTCG AATTCCTAGA GAAACAGAAT
    GAGCAGCTTT TGAAATCCTG TCACGCAGTG TCCCAAACGC AAGGCGAAGG AGATGCAGCA
    GATGGTGAAA TTGGAAGCCG AGATGGACCG CAGACCAGCA ACAGTTGTGT GAAACTCCAG
    GACACAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa44452
    Clone ID Related Accession (Same CDS sequence) NM_001166510.1
    Accession Version NM_001166510.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03058798.1, AAFC03052360.1, AAFC03094912.1, AAFC02124407.1, AAFC03094911.1 and AAFC03094913.1. On Oct 27, 2009 this sequence version replaced XM_001790207.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN01093727 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001159982.1
    CDS1..3585
    Translation

    Target ORF information:

    RefSeq Version NM_001166510.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C, beta 4 (PLCB4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001166510.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAAAC CTTATGAATT TAATTGGCAG AAGGAAGTTC CTTCCTTTTT GCAAGAAGGA 
    GCAGTTTTTG ACAGATATGA AGAGGAATCC TTTGTGTTTG AACCCAACTG CCTCTTCAAA
    GTAGATGAAT TTGGCTTCTT TCTGACCTGG AGAAGTGAAG GCAAGGAAGG CCAAGTGCTA
    GAATGCTCCC TCATCAACAG TGTTCGGCTG GGAGCCACAC CGAAGGATCC CAAAATTTTG
    GCTGCTCTTG AAGCTCTTGG CAAATCAGAA AATGATCTGG AAGGGCGGAT AGTTTGCGTC
    TGCAGTGGTG CAGATCTGGT GAACATCAGT TTCACGTACA TGGTGGCTGA AAATCCAGAA
    ATAACTAAGC AATGGGTCGA AGGCCTGAGA TCCATCATTC ACAACTTCAG GGCCAACAAC
    GTCAGCCCGA TGACGTGCCT CAAGAAGCAC TGGATGAAAT TGGCATTTAT GACCAACACA
    AATGGGAAAA TTCCAGTTAG GAGTATTACT AGAACCTTTG CATCGGGGAA AACAGAAAAG
    GTGATCTTTC AAGCACTCAA GGAGCTAGGT CTTCCCAGTG GAAAGAATGA TGAAATTGAG
    CCTGCCGCAT TTACTTATGA AAAGTTCTAT GAACTGACAC AAAAGATTTG TCCTCGGACA
    GATATAGAGG ATCTTTTCAA AAAAATCAAT GGAGACAAAA CTGATTATTT AACGGTAGAC
    CAATTAGTGA GCTTTCTAAA TGAACATCAA CGAGATCCTC GACTGAATGA AATTTTATTC
    CCATTTTATG ATGCTAAAAG GGCAATGCAG ATCATTGAGA TGTATGAGCC CGATGAAGAT
    TTGAAGAAAC AAGGCCTCAT ATCAAGTGAT GGGTTTTGCA GATACCTGAT GTCAGATGAA
    AACGCCCCCG TCTTCCTAGA TCGTCTAGAG CTGTACCAAG AAATGGACCA CCCTCTGGCT
    CACTACTTCA TCAGCTCTTC CCACAACACC TATCTCACTG GCAGACAGTT TGGTGGGAAA
    TCTTCAGTAG AAATGTACAG ACAGGTCCTC CTGGCTGGTT GCAGATGTGT TGAACTTGAC
    TGTTGGGATG GAAAAGGTGA GGACCAGGAA CCGATAATAA CTCACGGGAA AGCAATGTGC
    ACAGATATCC TTTTTAAGGA TGTTATTCAA GCCATCAAGG AAACTGCCTT TGTCACATCG
    GAATATCCTG TAATTCTCTC CTTTGAAAAT CACTGCAGCA AATATCAACA GTACAAGATG
    TCCAAATACT GTGAAGATCT ATTTGGAGAT CTCCTGTTGA AACAAGCACT TGAATCACAT
    CCACTGGAAC CAGGCAGACC CTTGCCATCC CCAAATGACC TCAAAAGAAA AATACTCATC
    AAAAACAAAC GACTGAAACC CGAGGTTGAA AAAAAACAGC TTGAAGCTTT GAAAAGCATG
    ATGGAAGCCG GAGAATCCGC TGCCCCGGTG AACATCTTAG AGGATGATAA TGAAGAGGAA
    ATAGAAAGTG CTGAGCAAGA GGAGGAAGCC CACCCTGAAT ACAAATATGG AAGTGAACTT
    TCTGCAGATG ACTTGGGTCA CAAGGAAGCT ATTGCCAATA GTGTCAAGAA GGGCCTGGTT
    ACCGTAGAAG ATGAGCAGGC GTGGATGGCA TCTTATAAAT ATGTGGGTGC TACCACTAAT
    ATCCATCCGT ATTTGTCCAC AATGATCAAC TATGCACAGC CTGTAAAGTT TCAAGGTTTC
    CATGTGGCTG AAGAACGCAA TATTCATTAT AACATGTCTT CCTTTAATGA GTCAGTCGGT
    CTAGGCTACT TGAAGACACA CGCCATTGAA TTTGTGAATT ATAATAAACG GCAAATGAGT
    CGCATTTACC CCAAGGGAGG CCGAGTGGAC TCCAGTAATT ACATGCCTCA GATTTTCTGG
    AACGCTGGCT GCCAGATGGT TTCGCTGAAC TATCAAACCC CAGATTTAGC GATGCAGTTG
    AATCAGGGGA AATTTGAGTA TAATGGATCG TGTGGGTACC TTCTCAAACC AGATTTCATG
    AGACGGCCTG ATCGAACATT TGATCCTTTC TCTGAAACGC CTGTTGATGG GGTTATTGCA
    GCCACTTGCT CCGTGCAGGT CATATCAGGT CAGTTCTTAT CAGATAAGAA AATTGGCACA
    TATGTAGAAG TGGATATGTA TGGGCTGCCC ACGGACACTA TCCGGAAGGA GTTCCGCACT
    CGCATGGTGA TGAACAACGG ACTCAATCCA GTTTACAATG AAGAATCATT TGTGTTTCGG
    AAGGTGATCC TCCCCGACCT GGCTGTCTTG AGGATCGCCG TGTATGACGA CAACAACAAG
    CTGATCGGCC AGAGGATTCT GCCCCTGGAT GGCCTCCAAG CAGGCTATCG ACACATATCC
    CTCCGGAATG AGGGAAATAA ACCTTTATCA CTGCCAACCA TTTTCTGCAA TATTGTCCTA
    AAAACATATG TGCCTGATGG ATTCGGAGAT ATTGTGGATG CTTTATCAGA TCCAAAGAAA
    TTTCTTTCCA TTACGGAAAA GAGAGCGGAT CAAATGAGAG CTATGGGCAT TGAAACTAGT
    GACATCGCCG ATGTGCCCAG TGACACTTCA AAGAACGACA AGAAAGGGAA AGCCAACACA
    GCCAAAGCGA ATGTGACCCC TCAGAGCAGC TCGGAGCTCA GGCCAACCAC CACAGCTGCC
    CTGGGGGCTG GCCTGGAGGC CAAGAAAGGT ATTGAACTGA TCCCTCAAGT GAGGATAGAA
    GATTTAAAGC AGATGAAGGC TTACTTGAAG CATTTAAAGA AACAACAGAA AGAGCTCAGT
    TCTTTAAAGA AGAAACATGC AAAGGAGCAC AGTACCATGC AGAAACTCCA TTGCACTCAA
    GTTGACAAGA TTGTGGCACA GTACGACAAG GAGAAATTGA CTCACGAGAA AATCCTAGAG
    AAGGCAATGA AGAAGAAAGG GGGAAGTAAT TGTCTTGAAA TGAAGAAAGA AACAGAAATT
    AAAATTCAGA CGCTGACATC AGATCACAAA TCTAAGGTCA AGGAGATTGT GGCACAGCAC
    ACAAAGGAGT GGTCAGACAT GATCAACACC CACAGTGCGG AGGAGCAGGA GATCCGGGAC
    CTGCACCTCA GCCAGCAGTG CGAGCTGCTC AGAAAGCTCC TGATCAGCGC CCACGAGCAG
    CAGACCCAGC AGCTGAAACT GTCCCATGAC AGGGAAAGCA AAGAGATGCG AGCACACCAA
    GCTAAGATTT CTATGGAAAA TAGCAAAGCC ATCAGCCAAG ATAAATCCAT CAAGAATAAA
    GCAGAACGGG AAAGGAGAGT CAGGGAGTTA AACAGCAGCA ACACTAAAAA GTTTCTGGAA
    GAAAGAAAGC GACTCGCCAT GAAGCAGTCC AAAGAAATGG ATCAGTTGAA AAAAGTCCAG
    CTTGAGCACC TCGAATTCCT AGAGAAACAG AATGAGCAGC TTTTGAAATC CTGTCACGCA
    GTGTCCCAAA CGCAAGGCGA AGGAGATGCA GCAGATGGTG AAATTGGAAG CCGAGATGGA
    CCGCAGACCA GCAACAGTTG TGTGAAACTC CAGGACACAA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.