NCAM1 cDNA ORF clone, Bos taurus(cattle)

The following NCAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa26211 XM_005215806.4
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa45272 XM_010812348.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa45273 XM_010812349.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa45274 XM_010812350.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa45275 XM_010812351.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45276 XM_010812352.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45277 XM_010812353.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45278 XM_010812354.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45279 XM_010812355.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45281 XM_010812357.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45282 XM_010812358.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45283 XM_010812359.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa41610 NM_174399.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa222638 XM_024975001.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222639 XM_024975002.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222640 XM_024975003.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222641 XM_024975004.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222642 XM_024975005.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222643 XM_024975006.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222644 XM_024975007.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222645 XM_024975008.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222646 XM_024975009.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222647 XM_024975010.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222648 XM_024975011.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222649 XM_024975012.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222650 XM_024975013.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222652 XM_024975015.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222629 XM_010812339.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222630 XM_010812341.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222631 XM_010812343.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222632 XM_010812344.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222633 XM_010812345.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222634 XM_010812346.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222635 XM_010812347.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222636 XM_024974999.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222637 XM_024975000.1
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa222651 XM_010812356.3
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa45263 XM_010812339.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45264 XM_010812340.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45265 XM_010812341.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45266 XM_010812342.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45267 XM_010812343.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45268 XM_010812344.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45269 XM_010812345.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45270 XM_010812346.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45271 XM_010812347.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45280 XM_010812356.2
Latest version!
Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa45274 XM_010812350.2 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa45279 XM_010812355.2 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45283 XM_010812359.2 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45277 XM_010812353.2 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45273 XM_010812349.2 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa26211 XM_005215806.3 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa45282 XM_010812358.2 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45278 XM_010812354.2 Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa26211
    Clone ID Related Accession (Same CDS sequence) XM_005215806.4 , XM_005215806.3
    Accession Version XM_005215806.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215806.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215863.1
    CDS283..2814
    Translation

    Target ORF information:

    RefSeq Version XM_005215806.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215806.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26211
    Clone ID Related Accession (Same CDS sequence) XM_005215806.4 , XM_005215806.3
    Accession Version XM_005215806.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215806.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215863.1
    CDS298..2829
    Translation

    Target ORF information:

    RefSeq Version XM_005215806.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215806.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45272
    Clone ID Related Accession (Same CDS sequence) XM_010812348.2
    Accession Version XM_010812348.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCGGCCTTC
    TCGAAAGATG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA GCGGACCCCG
    AATCACGACG GAGGGAAACA CACGGAGCCG AACGAGACCA CGCCGCTGAC GGAGCCCGAG
    AAGGGTCCCG TAGAAGCAAA GCCGGAGACA GAAACGAAGC CAGCGCCAGC CGAAGTCCAG
    ACGGTCCCCA ACGATGCCAC ACAAATAAAG GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810650.1
    CDS299..2929
    Translation

    Target ORF information:

    RefSeq Version XM_010812348.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812348.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCGGCCTTC
    TCGAAAGATG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA GCGGACCCCG
    AATCACGACG GAGGGAAACA CACGGAGCCG AACGAGACCA CGCCGCTGAC GGAGCCCGAG
    AAGGGTCCCG TAGAAGCAAA GCCGGAGACA GAAACGAAGC CAGCGCCAGC CGAAGTCCAG
    ACGGTCCCCA ACGATGCCAC ACAAATAAAG GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45273
    Clone ID Related Accession (Same CDS sequence) XM_010812349.3 , XM_010812349.2
    Accession Version XM_010812349.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812349.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACG AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810651.1
    CDS284..2848
    Translation

    Target ORF information:

    RefSeq Version XM_010812349.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812349.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACG AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45273
    Clone ID Related Accession (Same CDS sequence) XM_010812349.3 , XM_010812349.2
    Accession Version XM_010812349.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACG AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810651.1
    CDS298..2862
    Translation

    Target ORF information:

    RefSeq Version XM_010812349.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812349.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACG AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45274
    Clone ID Related Accession (Same CDS sequence) XM_010812350.2 , XM_010812350.3
    Accession Version XM_010812350.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA GATGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACGGA GCCGAACGAG
    ACCACGCCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACG
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810652.1
    CDS298..2832
    Translation

    Target ORF information:

    RefSeq Version XM_010812350.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812350.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA GATGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACGGA GCCGAACGAG
    ACCACGCCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACG
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45274
    Clone ID Related Accession (Same CDS sequence) XM_010812350.2 , XM_010812350.3
    Accession Version XM_010812350.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812350.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA GATGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACGGA GCCGAACGAG
    ACCACGCCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACG
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810652.1
    CDS283..2817
    Translation

    Target ORF information:

    RefSeq Version XM_010812350.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812350.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA GATGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACGGA GCCGAACGAG
    ACCACGCCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACG
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45275
    Clone ID Related Accession (Same CDS sequence) XM_010812351.2
    Accession Version XM_010812351.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCGACCT TGGGAGGCTC CTCCGCATCC
    TACACCTTTG TCTCATTGCT TTTCTCTGCA GTGACTCTTC TCTTGCTCTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810653.1
    CDS300..2573
    Translation

    Target ORF information:

    RefSeq Version XM_010812351.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCGACCT TGGGAGGCTC CTCCGCATCC
    TACACCTTTG TCTCATTGCT TTTCTCTGCA GTGACTCTTC TCTTGCTCTG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45276
    Clone ID Related Accession (Same CDS sequence) XM_010812352.2
    Accession Version XM_010812352.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCGACCTTGG GAGGCTCCTC CGCATCCTAC
    ACCTTTGTCT CATTGCTTTT CTCTGCAGTG ACTCTTCTCT TGCTCTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810654.1
    CDS300..2570
    Translation

    Target ORF information:

    RefSeq Version XM_010812352.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812352.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCGACCTTGG GAGGCTCCTC CGCATCCTAC
    ACCTTTGTCT CATTGCTTTT CTCTGCAGTG ACTCTTCTCT TGCTCTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45277
    Clone ID Related Accession (Same CDS sequence) XM_010812353.2 , XM_010812353.3
    Accession Version XM_010812353.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCCGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810655.1
    CDS299..2506
    Translation

    Target ORF information:

    RefSeq Version XM_010812353.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812353.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCCGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45277
    Clone ID Related Accession (Same CDS sequence) XM_010812353.2 , XM_010812353.3
    Accession Version XM_010812353.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCCGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810655.1
    CDS284..2491
    Translation

    Target ORF information:

    RefSeq Version XM_010812353.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812353.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCCGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45278
    Clone ID Related Accession (Same CDS sequence) XM_010812354.2 , XM_010812354.3
    Accession Version XM_010812354.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCGACC TTGGGAGGCT CCTCCGCATC CTACACCTTT
    GTCTCATTGC TTTTCTCTGC AGTGACTCTT CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810656.1
    CDS298..2502
    Translation

    Target ORF information:

    RefSeq Version XM_010812354.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812354.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCGACC TTGGGAGGCT CCTCCGCATC CTACACCTTT
    GTCTCATTGC TTTTCTCTGC AGTGACTCTT CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45278
    Clone ID Related Accession (Same CDS sequence) XM_010812354.2 , XM_010812354.3
    Accession Version XM_010812354.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCGACC TTGGGAGGCT CCTCCGCATC CTACACCTTT
    GTCTCATTGC TTTTCTCTGC AGTGACTCTT CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810656.1
    CDS283..2487
    Translation

    Target ORF information:

    RefSeq Version XM_010812354.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812354.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCGACC TTGGGAGGCT CCTCCGCATC CTACACCTTT
    GTCTCATTGC TTTTCTCTGC AGTGACTCTT CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45279
    Clone ID Related Accession (Same CDS sequence) XM_010812355.2 , XM_010812355.3
    Accession Version XM_010812355.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810657.1
    CDS298..2475
    Translation

    Target ORF information:

    RefSeq Version XM_010812355.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45279
    Clone ID Related Accession (Same CDS sequence) XM_010812355.2 , XM_010812355.3
    Accession Version XM_010812355.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810657.1
    CDS337..2514
    Translation

    Target ORF information:

    RefSeq Version XM_010812355.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812355.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45281
    Clone ID Related Accession (Same CDS sequence) XM_010812357.2
    Accession Version XM_010812357.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATAAAGGG
    GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAGGTG
    AAGCTGATCA AGCAAGACGA CGGCGGCTCC CCCATCCGAC ACTACCTGGT CAAATACCGA
    GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
    CTCAAGTCCC TGGACTGGAA TGCCGAGTAC GAGGTCTATG TGGTGGCCGA GAACCAGCAG
    GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
    GCGACCTTGG GAGGCTCCTC CGCATCCTAC ACCTTTGTCT CATTGCTTTT CTCTGCAGTG
    ACTCTTCTCT TGCTCTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810659.1
    CDS299..2539
    Translation

    Target ORF information:

    RefSeq Version XM_010812357.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812357.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATAAAGGG
    GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAGGTG
    AAGCTGATCA AGCAAGACGA CGGCGGCTCC CCCATCCGAC ACTACCTGGT CAAATACCGA
    GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
    CTCAAGTCCC TGGACTGGAA TGCCGAGTAC GAGGTCTATG TGGTGGCCGA GAACCAGCAG
    GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
    GCGACCTTGG GAGGCTCCTC CGCATCCTAC ACCTTTGTCT CATTGCTTTT CTCTGCAGTG
    ACTCTTCTCT TGCTCTGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45282
    Clone ID Related Accession (Same CDS sequence) XM_010812358.3 , XM_010812358.2
    Accession Version XM_010812358.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810660.1
    CDS283..2460
    Translation

    Target ORF information:

    RefSeq Version XM_010812358.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812358.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45282
    Clone ID Related Accession (Same CDS sequence) XM_010812358.3 , XM_010812358.2
    Accession Version XM_010812358.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812358.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810660.1
    CDS299..2476
    Translation

    Target ORF information:

    RefSeq Version XM_010812358.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812358.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCG
    ACCTTGGGAG GCTCCTCCGC ATCCTACACC TTTGTCTCAT TGCTTTTCTC TGCAGTGACT
    CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45283
    Clone ID Related Accession (Same CDS sequence) XM_010812359.2 , XM_010812359.3
    Accession Version XM_010812359.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCGACC
    TTGGGAGGCT CCTCCGCATC CTACACCTTT GTCTCATTGC TTTTCTCTGC AGTGACTCTT
    CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810661.1
    CDS298..2472
    Translation

    Target ORF information:

    RefSeq Version XM_010812359.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812359.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCGACC
    TTGGGAGGCT CCTCCGCATC CTACACCTTT GTCTCATTGC TTTTCTCTGC AGTGACTCTT
    CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45283
    Clone ID Related Accession (Same CDS sequence) XM_010812359.2 , XM_010812359.3
    Accession Version XM_010812359.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCGACC
    TTGGGAGGCT CCTCCGCATC CTACACCTTT GTCTCATTGC TTTTCTCTGC AGTGACTCTT
    CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810661.1
    CDS326..2500
    Translation

    Target ORF information:

    RefSeq Version XM_010812359.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812359.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCGACC
    TTGGGAGGCT CCTCCGCATC CTACACCTTT GTCTCATTGC TTTTCTCTGC AGTGACTCTT
    CTCTTGCTCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41610
    Clone ID Related Accession (Same CDS sequence) NM_174399.1
    Accession Version NM_174399.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X16451.1. ##Evidence-Data-START## Transcript exon combination :: X16451.1, BC151709.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TTGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCGTCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTNCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACTT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACGGAGCC GAACGAGACC ACGCCGCTGA CGGAGCCCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACGAAG CCTGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGTGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776824.1
    CDS350..2911
    Translation

    Target ORF information:

    RefSeq Version NM_174399.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174399.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TTGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCGTCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTNCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACTT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACGGAGCC GAACGAGACC ACGCCGCTGA CGGAGCCCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACGAAG CCTGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGTGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222638
    Clone ID Related Accession (Same CDS sequence) XM_024975001.1
    Accession Version XM_024975001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAAGCG GCGCACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACGGAGC CGAACGAGAC CACGCCGCTG ACGGAGCCCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACGAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830769.1
    CDS286..2958
    Translation

    Target ORF information:

    RefSeq Version XM_024975001.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAAGCG GCGCACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACGGAGC CGAACGAGAC CACGCCGCTG ACGGAGCCCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACGAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222639
    Clone ID Related Accession (Same CDS sequence) XM_024975002.1
    Accession Version XM_024975002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAAGCGGCG CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCCAACGG CAGCCCCACC TCGGGCCTGA GCACTGGCGC CATCGTGGGC
    ATCCTCGTCG TGACCTTCGT CCTGCTCCTG GTGGCCGTGG ACGTCACCTG CTACTTCCTG
    AACAAGTGTG GCCTGCTCAT GTGCATTGCC GTCAACCTGT GTGGCAAAGC CGGGCCCGGA
    GCCAAGGGCA AGGACATGGA GGAGGGCAAA GCGGCCTTCT CGAAAGATGA GTCCAAGGAG
    CCTATCGTGG AGGTGCGAAC GGAGGAGGAG CGGACCCCGA ATCACGACGG AGGGAAACAC
    ACGGAGCCGA ACGAGACCAC GCCGCTGACG GAGCCCGAGA AGGGTCCCGT AGAAGCAAAG
    CCGGAGACAG AAACGAAGCC AGCGCCAGCC GAAGTCCAGA CGGTCCCCAA CGATGCCACA
    CAAATAAAGG AGAATGAGAG CAAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830770.1
    CDS286..2955
    Translation

    Target ORF information:

    RefSeq Version XM_024975002.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAAGCGGCG CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCCAACGG CAGCCCCACC TCGGGCCTGA GCACTGGCGC CATCGTGGGC
    ATCCTCGTCG TGACCTTCGT CCTGCTCCTG GTGGCCGTGG ACGTCACCTG CTACTTCCTG
    AACAAGTGTG GCCTGCTCAT GTGCATTGCC GTCAACCTGT GTGGCAAAGC CGGGCCCGGA
    GCCAAGGGCA AGGACATGGA GGAGGGCAAA GCGGCCTTCT CGAAAGATGA GTCCAAGGAG
    CCTATCGTGG AGGTGCGAAC GGAGGAGGAG CGGACCCCGA ATCACGACGG AGGGAAACAC
    ACGGAGCCGA ACGAGACCAC GCCGCTGACG GAGCCCGAGA AGGGTCCCGT AGAAGCAAAG
    CCGGAGACAG AAACGAAGCC AGCGCCAGCC GAAGTCCAGA CGGTCCCCAA CGATGCCACA
    CAAATAAAGG AGAATGAGAG CAAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222640
    Clone ID Related Accession (Same CDS sequence) XM_024975003.1
    Accession Version XM_024975003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCGGCCTTCT CGAAAGATGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAG
    CGGACCCCGA ATCACGACGG AGGGAAACAC ACGGAGCCGA ACGAGACCAC GCCGCTGACG
    GAGCCCGAGA AGGGTCCCGT AGAAGCAAAG CCGGAGACAG AAACGAAGCC AGCGCCAGCC
    GAAGTCCAGA CGGTCCCCAA CGATGCCACA CAAATAAAGG AGAATGAGAG CAAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830771.1
    CDS285..2864
    Translation

    Target ORF information:

    RefSeq Version XM_024975003.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCGGCCTTCT CGAAAGATGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAG
    CGGACCCCGA ATCACGACGG AGGGAAACAC ACGGAGCCGA ACGAGACCAC GCCGCTGACG
    GAGCCCGAGA AGGGTCCCGT AGAAGCAAAG CCGGAGACAG AAACGAAGCC AGCGCCAGCC
    GAAGTCCAGA CGGTCCCCAA CGATGCCACA CAAATAAAGG AGAATGAGAG CAAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222641
    Clone ID Related Accession (Same CDS sequence) XM_024975004.1
    Accession Version XM_024975004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGCTCTCCTC CGAGTGGAAA
    CCAGAGATCA GGCTCCCGTC TGGCAGTGAC CACGTCATGC TCAAGTCCCT GGACTGGAAT
    GCCGAGTACG AGGTCTATGT GGTGGCCGAG AACCAGCAGG GCAAGTCCAA AGCGGCGCAC
    TTTGTGTTCA GGACCTCGGC CCAGCCCACA GCCATCCCAG CCAACGGCAG CCCCACCTCG
    GGCCTGAGCA CTGGCGCCAT CGTGGGCATC CTCGTCGTGA CCTTCGTCCT GCTCCTGGTG
    GCCGTGGACG TCACCTGCTA CTTCCTGAAC AAGTGTGGCC TGCTCATGTG CATTGCCGTC
    AACCTGTGTG GCAAAGCCGG GCCCGGAGCC AAGGGCAAGG ACATGGAGGA GGGCAAAGCG
    GCCTTCTCGA AAGATGAGTC CAAGGAGCCT ATCGTGGAGG TGCGAACGGA GGAGGAGCGG
    ACCCCGAATC ACGACGGAGG GAAACACACG GAGCCGAACG AGACCACGCC GCTGACGGAG
    CCCGAGAAGG GTCCCGTAGA AGCAAAGCCG GAGACAGAAA CGAAGCCAGC GCCAGCCGAA
    GTCCAGACGG TCCCCAACGA TGCCACACAA ATAAAGGAGA ATGAGAGCAA AGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830772.1
    CDS285..2861
    Translation

    Target ORF information:

    RefSeq Version XM_024975004.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGCTCTCCTC CGAGTGGAAA
    CCAGAGATCA GGCTCCCGTC TGGCAGTGAC CACGTCATGC TCAAGTCCCT GGACTGGAAT
    GCCGAGTACG AGGTCTATGT GGTGGCCGAG AACCAGCAGG GCAAGTCCAA AGCGGCGCAC
    TTTGTGTTCA GGACCTCGGC CCAGCCCACA GCCATCCCAG CCAACGGCAG CCCCACCTCG
    GGCCTGAGCA CTGGCGCCAT CGTGGGCATC CTCGTCGTGA CCTTCGTCCT GCTCCTGGTG
    GCCGTGGACG TCACCTGCTA CTTCCTGAAC AAGTGTGGCC TGCTCATGTG CATTGCCGTC
    AACCTGTGTG GCAAAGCCGG GCCCGGAGCC AAGGGCAAGG ACATGGAGGA GGGCAAAGCG
    GCCTTCTCGA AAGATGAGTC CAAGGAGCCT ATCGTGGAGG TGCGAACGGA GGAGGAGCGG
    ACCCCGAATC ACGACGGAGG GAAACACACG GAGCCGAACG AGACCACGCC GCTGACGGAG
    CCCGAGAAGG GTCCCGTAGA AGCAAAGCCG GAGACAGAAA CGAAGCCAGC GCCAGCCGAA
    GTCCAGACGG TCCCCAACGA TGCCACACAA ATAAAGGAGA ATGAGAGCAA AGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222642
    Clone ID Related Accession (Same CDS sequence) XM_024975005.1
    Accession Version XM_024975005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACG AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830773.1
    CDS285..2849
    Translation

    Target ORF information:

    RefSeq Version XM_024975005.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGAAGGGT
    CCCGTAGAAG CAAAGCCGGA GACAGAAACG AAGCCAGCGC CAGCCGAAGT CCAGACGGTC
    CCCAACGATG CCACACAAAT AAAGGAGAAT GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222643
    Clone ID Related Accession (Same CDS sequence) XM_024975006.1
    Accession Version XM_024975006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    AAGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
    CTCAAGTCCC TGGACTGGAA TGCCGAGTAC GAGGTCTATG TGGTGGCCGA GAACCAGCAG
    GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
    GCCAACGGCA GCCCCACCTC GGGCCTGAGC ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG
    ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC GTCACCTGCT ACTTCCTGAA CAAGTGTGGC
    CTGCTCATGT GCATTGCCGT CAACCTGTGT GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG
    GACATGGAGG AGGGCAAAGC GGCCTTCTCG AAAGATGAGT CCAAGGAGCC TATCGTGGAG
    GTGCGAACGG AGGAGGAGCG GACCCCGAAT CACGACGGAG GGAAACACAC GGAGCCGAAC
    GAGACCACGC CGCTGACGGA GCCCGAGAAG GGTCCCGTAG AAGCAAAGCC GGAGACAGAA
    ACGAAGCCAG CGCCAGCCGA AGTCCAGACG GTCCCCAACG ATGCCACACA AATAAAGGAG
    AATGAGAGCA AAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830774.1
    CDS284..2821
    Translation

    Target ORF information:

    RefSeq Version XM_024975006.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    AAGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
    CTCAAGTCCC TGGACTGGAA TGCCGAGTAC GAGGTCTATG TGGTGGCCGA GAACCAGCAG
    GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
    GCCAACGGCA GCCCCACCTC GGGCCTGAGC ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG
    ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC GTCACCTGCT ACTTCCTGAA CAAGTGTGGC
    CTGCTCATGT GCATTGCCGT CAACCTGTGT GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG
    GACATGGAGG AGGGCAAAGC GGCCTTCTCG AAAGATGAGT CCAAGGAGCC TATCGTGGAG
    GTGCGAACGG AGGAGGAGCG GACCCCGAAT CACGACGGAG GGAAACACAC GGAGCCGAAC
    GAGACCACGC CGCTGACGGA GCCCGAGAAG GGTCCCGTAG AAGCAAAGCC GGAGACAGAA
    ACGAAGCCAG CGCCAGCCGA AGTCCAGACG GTCCCCAACG ATGCCACACA AATAAAGGAG
    AATGAGAGCA AAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222644
    Clone ID Related Accession (Same CDS sequence) XM_024975007.1
    Accession Version XM_024975007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAAGCG GCGCACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCGAC CTTGGGAGGC TCCTCCGCAT CCTACACCTT TGTCTCATTG
    CTTTTCTCTG CAGTGACTCT TCTCTTGCTC TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830775.1
    CDS285..2600
    Translation

    Target ORF information:

    RefSeq Version XM_024975007.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAAGCG GCGCACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCGAC CTTGGGAGGC TCCTCCGCAT CCTACACCTT TGTCTCATTG
    CTTTTCTCTG CAGTGACTCT TCTCTTGCTC TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222645
    Clone ID Related Accession (Same CDS sequence) XM_024975008.1
    Accession Version XM_024975008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAAGCGGCG CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCGACCTT GGGAGGCTCC TCCGCATCCT ACACCTTTGT CTCATTGCTT
    TTCTCTGCAG TGACTCTTCT CTTGCTCTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830776.1
    CDS285..2597
    Translation

    Target ORF information:

    RefSeq Version XM_024975008.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGCTCTC CTCCGAGTGG AAACCAGAGA TCAGGCTCCC GTCTGGCAGT
    GACCACGTCA TGCTCAAGTC CCTGGACTGG AATGCCGAGT ACGAGGTCTA TGTGGTGGCC
    GAGAACCAGC AGGGCAAGTC CAAAGCGGCG CACTTTGTGT TCAGGACCTC GGCCCAGCCC
    ACAGCCATCC CAGCGACCTT GGGAGGCTCC TCCGCATCCT ACACCTTTGT CTCATTGCTT
    TTCTCTGCAG TGACTCTTCT CTTGCTCTGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222646
    Clone ID Related Accession (Same CDS sequence) XM_024975009.1
    Accession Version XM_024975009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCCTC CTGCTCTTAC AACCACCGAA GCAACTAAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAAGCG
    GCGCACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCGAC CTTGGGAGGC
    TCCTCCGCAT CCTACACCTT TGTCTCATTG CTTTTCTCTG CAGTGACTCT TCTCTTGCTC
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830777.1
    CDS284..2569
    Translation

    Target ORF information:

    RefSeq Version XM_024975009.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCCTC CTGCTCTTAC AACCACCGAA GCAACTAAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAAGCG
    GCGCACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCGAC CTTGGGAGGC
    TCCTCCGCAT CCTACACCTT TGTCTCATTG CTTTTCTCTG CAGTGACTCT TCTCTTGCTC
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222647
    Clone ID Related Accession (Same CDS sequence) XM_024975010.1
    Accession Version XM_024975010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCCTC CTGCTCTTAC AACCACCGAA GCAACTAAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCCGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830778.1
    CDS284..2566
    Translation

    Target ORF information:

    RefSeq Version XM_024975010.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATACAACC
    TGGCCTCCTC CTGCTCTTAC AACCACCGAA GCAACTAAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCCGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222648
    Clone ID Related Accession (Same CDS sequence) XM_024975011.1
    Accession Version XM_024975011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCCGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830779.1
    CDS284..2506
    Translation

    Target ORF information:

    RefSeq Version XM_024975011.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCGACCTT GGGAGGCTCC
    TCCGCATCCT ACACCTTTGT CTCATTGCTT TTCTCTGCAG TGACTCTTCT CTTGCTCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222649
    Clone ID Related Accession (Same CDS sequence) XM_024975012.1
    Accession Version XM_024975012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGCTCTCCTC CGAGTGGAAA
    CCAGAGATCA GGCTCCCGTC TGGCAGTGAC CACGTCATGC TCAAGTCCCT GGACTGGAAT
    GCCGAGTACG AGGTCTATGT GGTGGCCGAG AACCAGCAGG GCAAGTCCAA AGCGGCGCAC
    TTTGTGTTCA GGACCTCGGC CCAGCCCACA GCCATCCCAG CGACCTTGGG AGGCTCCTCC
    GCATCCTACA CCTTTGTCTC ATTGCTTTTC TCTGCAGTGA CTCTTCTCTT GCTCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830780.1
    CDS284..2503
    Translation

    Target ORF information:

    RefSeq Version XM_024975012.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGCTCTCCTC CGAGTGGAAA
    CCAGAGATCA GGCTCCCGTC TGGCAGTGAC CACGTCATGC TCAAGTCCCT GGACTGGAAT
    GCCGAGTACG AGGTCTATGT GGTGGCCGAG AACCAGCAGG GCAAGTCCAA AGCGGCGCAC
    TTTGTGTTCA GGACCTCGGC CCAGCCCACA GCCATCCCAG CGACCTTGGG AGGCTCCTCC
    GCATCCTACA CCTTTGTCTC ATTGCTTTTC TCTGCAGTGA CTCTTCTCTT GCTCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222650
    Clone ID Related Accession (Same CDS sequence) XM_024975013.1
    Accession Version XM_024975013.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCCGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830781.1
    CDS283..2490
    Translation

    Target ORF information:

    RefSeq Version XM_024975013.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCG ACCTTGGGAG GCTCCTCCGC ATCCTACACC
    TTTGTCTCAT TGCTTTTCTC TGCAGTGACT CTTCTCTTGC TCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222652
    Clone ID Related Accession (Same CDS sequence) XM_024975015.1
    Accession Version XM_024975015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA GATGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACGGA GCCGAACGAG
    ACCACGCCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACG
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830783.1
    CDS284..2818
    Translation

    Target ORF information:

    RefSeq Version XM_024975015.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGAAG
    CTCTCCTCCG AGTGGAAACC AGAGATCAGG CTCCCGTCTG GCAGTGACCA CGTCATGCTC
    AAGTCCCTGG ACTGGAATGC CGAGTACGAG GTCTATGTGG TGGCCGAGAA CCAGCAGGGC
    AAGTCCAAAG CGGCGCACTT TGTGTTCAGG ACCTCGGCCC AGCCCACAGC CATCCCAGCC
    AACGGCAGCC CCACCTCGGG CCTGAGCACT GGCGCCATCG TGGGCATCCT CGTCGTGACC
    TTCGTCCTGC TCCTGGTGGC CGTGGACGTC ACCTGCTACT TCCTGAACAA GTGTGGCCTG
    CTCATGTGCA TTGCCGTCAA CCTGTGTGGC AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC
    ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA GATGAGTCCA AGGAGCCTAT CGTGGAGGTG
    CGAACGGAGG AGGAGCGGAC CCCGAATCAC GACGGAGGGA AACACACGGA GCCGAACGAG
    ACCACGCCGC TGACGGAGCC CGAGAAGGGT CCCGTAGAAG CAAAGCCGGA GACAGAAACG
    AAGCCAGCGC CAGCCGAAGT CCAGACGGTC CCCAACGATG CCACACAAAT AAAGGAGAAT
    GAGAGCAAAG CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222629
    Clone ID Related Accession (Same CDS sequence) XM_010812339.3
    Accession Version XM_010812339.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812339.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCGGCCTTC
    TCGAAAGATG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA GCGGACCCCG
    AATCACGACG GAGGGAAACA CACGGAGCCG AACGAGACCA CGCCGCTGAC GGAGCCCGAG
    CTGCCTGCTG ACACCACAGC CACTGTGGAG GACATGCTGC CTTCTGTCAC CACCGTCACC
    GCTAACTCTG ACACTATCAC CGAAACCTTT GCCACTGCTC AGAACAGCCC CACCAGTGAG
    ACCACCACCC TGACCTCCAG TATCGCCCCC CCGGTCGCGG CCACCCCTGA TTCAAACTCT
    GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG GGGACCGGCG TCACTGCCGC CTCCCCGGCC
    CCGGCCCCAG CCCCCAAGGT CGCCCCCCTC GTTGACCTGA GCGACCCTCC GGCCTCAGCC
    CCGGCCGCCA ACAACCTGTC CTCTAGCGTC CTGGCCAACC AGGGGGCCGT GCTCAGCCCC
    AGCACCCCGG CCAGCGCGGG CGAGGCCCCC AAGACCCCTC CAGCCAGCAA GGCAGTCTCC
    CCGTCAGCAG CCAGTCCCCT GGCAGCAGCC CCGACAGCCC CTGCCCCGGA AGCCCCCCAG
    GCCAAGCAGG AGGCTCCCGG CACCAAAGGT CCGGACCCCG AGCCCACCCA GCCCGGCGCC
    TCGAAGAGCC CACCCGAGGC CGCCAGGGCC CTCGCCAGCC CGAAGAGCGA GGCAGCCTCC
    GTCAGCACCA CAAACCCTGC CCAGGGCGAG GACTTTAAAA TGGACGAAGG GAACTTCAAG
    ACCCCAGATA TTGACCTTGC CAAGGATGTT TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC
    GCTGGGGCCA GTGGACAAGC CTCTGAGCTT GCTCCTTCCA CGGCCGACGG CTCTCTTCCG
    CCTGCACCAG TCAAGACCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810641.2
    CDS292..3723
    Translation

    Target ORF information:

    RefSeq Version XM_010812339.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812339.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCGGCCTTC
    TCGAAAGATG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA GCGGACCCCG
    AATCACGACG GAGGGAAACA CACGGAGCCG AACGAGACCA CGCCGCTGAC GGAGCCCGAG
    CTGCCTGCTG ACACCACAGC CACTGTGGAG GACATGCTGC CTTCTGTCAC CACCGTCACC
    GCTAACTCTG ACACTATCAC CGAAACCTTT GCCACTGCTC AGAACAGCCC CACCAGTGAG
    ACCACCACCC TGACCTCCAG TATCGCCCCC CCGGTCGCGG CCACCCCTGA TTCAAACTCT
    GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG GGGACCGGCG TCACTGCCGC CTCCCCGGCC
    CCGGCCCCAG CCCCCAAGGT CGCCCCCCTC GTTGACCTGA GCGACCCTCC GGCCTCAGCC
    CCGGCCGCCA ACAACCTGTC CTCTAGCGTC CTGGCCAACC AGGGGGCCGT GCTCAGCCCC
    AGCACCCCGG CCAGCGCGGG CGAGGCCCCC AAGACCCCTC CAGCCAGCAA GGCAGTCTCC
    CCGTCAGCAG CCAGTCCCCT GGCAGCAGCC CCGACAGCCC CTGCCCCGGA AGCCCCCCAG
    GCCAAGCAGG AGGCTCCCGG CACCAAAGGT CCGGACCCCG AGCCCACCCA GCCCGGCGCC
    TCGAAGAGCC CACCCGAGGC CGCCAGGGCC CTCGCCAGCC CGAAGAGCGA GGCAGCCTCC
    GTCAGCACCA CAAACCCTGC CCAGGGCGAG GACTTTAAAA TGGACGAAGG GAACTTCAAG
    ACCCCAGATA TTGACCTTGC CAAGGATGTT TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC
    GCTGGGGCCA GTGGACAAGC CTCTGAGCTT GCTCCTTCCA CGGCCGACGG CTCTCTTCCG
    CCTGCACCAG TCAAGACCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222630
    Clone ID Related Accession (Same CDS sequence) XM_010812341.3
    Accession Version XM_010812341.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812341.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG ACCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCAGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC AGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810643.2
    CDS292..3720
    Translation

    Target ORF information:

    RefSeq Version XM_010812341.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812341.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG ACCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCAGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC AGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222631
    Clone ID Related Accession (Same CDS sequence) XM_010812343.3
    Accession Version XM_010812343.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812343.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAAGCG
    GCGCACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAGCGGACCC CGAATCACGA CGGAGGGAAA CACACGGAGC CGAACGAGAC CACGCCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GATTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTCGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGACCCC TCCAGCCAGC
    AAGGCAGTCT CCCCGTCAGC AGCCAGTCCC CTGGCAGCAG CCCCGACAGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCAGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCAGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGTCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACGA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810645.2
    CDS292..3675
    Translation

    Target ORF information:

    RefSeq Version XM_010812343.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812343.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAAGCG
    GCGCACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAGCGGACCC CGAATCACGA CGGAGGGAAA CACACGGAGC CGAACGAGAC CACGCCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GATTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTCGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGACCCC TCCAGCCAGC
    AAGGCAGTCT CCCCGTCAGC AGCCAGTCCC CTGGCAGCAG CCCCGACAGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCAGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCAGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGTCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACGA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222632
    Clone ID Related Accession (Same CDS sequence) XM_010812344.3
    Accession Version XM_010812344.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCGGCCTTCT CGAAAGATGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAG
    CGGACCCCGA ATCACGACGG AGGGAAACAC ACGGAGCCGA ACGAGACCAC GCCGCTGACG
    GAGCCCGAGC TGCCTGCTGA CACCACAGCC ACTGTGGAGG ACATGCTGCC TTCTGTCACC
    ACCGTCACCG CTAACTCTGA CACTATCACC GAAACCTTTG CCACTGCTCA GAACAGCCCC
    ACCAGTGAGA CCACCACCCT GACCTCCAGT ATCGCCCCCC CGGTCGCGGC CACCCCTGAT
    TCAAACTCTG TGCCCGCCGG CCAGGCCACC CCGTCCAAGG GGACCGGCGT CACTGCCGCC
    TCCCCGGCCC CGGCCCCAGC CCCCAAGGTC GCCCCCCTCG TTGACCTGAG CGACCCTCCG
    GCCTCAGCCC CGGCCGCCAA CAACCTGTCC TCTAGCGTCC TGGCCAACCA GGGGGCCGTG
    CTCAGCCCCA GCACCCCGGC CAGCGCGGGC GAGGCCCCCA AGACCCCTCC AGCCAGCAAG
    GCAGTCTCCC CGTCAGCAGC CAGTCCCCTG GCAGCAGCCC CGACAGCCCC TGCCCCGGAA
    GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC ACCAAAGGTC CGGACCCCGA GCCCACCCAG
    CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC GCCAGGGCCC TCGCCAGCCC GAAGAGCGAG
    GCAGCCTCCG TCAGCACCAC AAACCCTGCC CAGGGCGAGG ACTTTAAAAT GGACGAAGGG
    AACTTCAAGA CCCCAGATAT TGACCTTGCC AAGGATGTTT TTGCAGCCCT GGGCTCTCCT
    GCTCCCGCCG CTGGGGCCAG TGGACAAGCC TCTGAGCTTG CTCCTTCCAC GGCCGACGGC
    TCTCTTCCGC CTGCACCAGT CAAGACCGAG AAGGGTCCCG TAGAAGCAAA GCCGGAGACA
    GAAACGAAGC CAGCGCCAGC CGAAGTCCAG ACGGTCCCCA ACGATGCCAC ACAAATAAAG
    GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810646.2
    CDS292..3672
    Translation

    Target ORF information:

    RefSeq Version XM_010812344.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812344.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCGGCCTTCT CGAAAGATGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAG
    CGGACCCCGA ATCACGACGG AGGGAAACAC ACGGAGCCGA ACGAGACCAC GCCGCTGACG
    GAGCCCGAGC TGCCTGCTGA CACCACAGCC ACTGTGGAGG ACATGCTGCC TTCTGTCACC
    ACCGTCACCG CTAACTCTGA CACTATCACC GAAACCTTTG CCACTGCTCA GAACAGCCCC
    ACCAGTGAGA CCACCACCCT GACCTCCAGT ATCGCCCCCC CGGTCGCGGC CACCCCTGAT
    TCAAACTCTG TGCCCGCCGG CCAGGCCACC CCGTCCAAGG GGACCGGCGT CACTGCCGCC
    TCCCCGGCCC CGGCCCCAGC CCCCAAGGTC GCCCCCCTCG TTGACCTGAG CGACCCTCCG
    GCCTCAGCCC CGGCCGCCAA CAACCTGTCC TCTAGCGTCC TGGCCAACCA GGGGGCCGTG
    CTCAGCCCCA GCACCCCGGC CAGCGCGGGC GAGGCCCCCA AGACCCCTCC AGCCAGCAAG
    GCAGTCTCCC CGTCAGCAGC CAGTCCCCTG GCAGCAGCCC CGACAGCCCC TGCCCCGGAA
    GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC ACCAAAGGTC CGGACCCCGA GCCCACCCAG
    CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC GCCAGGGCCC TCGCCAGCCC GAAGAGCGAG
    GCAGCCTCCG TCAGCACCAC AAACCCTGCC CAGGGCGAGG ACTTTAAAAT GGACGAAGGG
    AACTTCAAGA CCCCAGATAT TGACCTTGCC AAGGATGTTT TTGCAGCCCT GGGCTCTCCT
    GCTCCCGCCG CTGGGGCCAG TGGACAAGCC TCTGAGCTTG CTCCTTCCAC GGCCGACGGC
    TCTCTTCCGC CTGCACCAGT CAAGACCGAG AAGGGTCCCG TAGAAGCAAA GCCGGAGACA
    GAAACGAAGC CAGCGCCAGC CGAAGTCCAG ACGGTCCCCA ACGATGCCAC ACAAATAAAG
    GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222633
    Clone ID Related Accession (Same CDS sequence) XM_010812345.3
    Accession Version XM_010812345.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG ACCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCAGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC AGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810647.2
    CDS292..3660
    Translation

    Target ORF information:

    RefSeq Version XM_010812345.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812345.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG ACCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCAGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC AGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222634
    Clone ID Related Accession (Same CDS sequence) XM_010812346.3
    Accession Version XM_010812346.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGATTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTCGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGACC CCTCCAGCCA GCAAGGCAGT CTCCCCGTCA
    GCAGCCAGTC CCCTGGCAGC AGCCCCGACA GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCAG GGCCCTCGCC AGCCCGAAGA GCGAGGCAGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGTCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC GAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810648.2
    CDS292..3657
    Translation

    Target ORF information:

    RefSeq Version XM_010812346.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812346.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGATTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTCGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGACC CCTCCAGCCA GCAAGGCAGT CTCCCCGTCA
    GCAGCCAGTC CCCTGGCAGC AGCCCCGACA GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCAG GGCCCTCGCC AGCCCGAAGA GCGAGGCAGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGTCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC GAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222635
    Clone ID Related Accession (Same CDS sequence) XM_010812347.3
    Accession Version XM_010812347.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812347.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACGGAGCC GAACGAGACC ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT
    GACACCACAG CCACTGTGGA GGACATGCTG CCTTCTGTCA CCACCGTCAC CGCTAACTCT
    GACACTATCA CCGAAACCTT TGCCACTGCT CAGAACAGCC CCACCAGTGA GACCACCACC
    CTGACCTCCA GTATCGCCCC CCCGGTCGCG GCCACCCCTG ATTCAAACTC TGTGCCCGCC
    GGCCAGGCCA CCCCGTCCAA GGGGACCGGC GTCACTGCCG CCTCCCCGGC CCCGGCCCCA
    GCCCCCAAGG TCGCCCCCCT CGTTGACCTG AGCGACCCTC CGGCCTCAGC CCCGGCCGCC
    AACAACCTGT CCTCTAGCGT CCTGGCCAAC CAGGGGGCCG TGCTCAGCCC CAGCACCCCG
    GCCAGCGCGG GCGAGGCCCC CAAGACCCCT CCAGCCAGCA AGGCAGTCTC CCCGTCAGCA
    GCCAGTCCCC TGGCAGCAGC CCCGACAGCC CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG
    GAGGCTCCCG GCACCAAAGG TCCGGACCCC GAGCCCACCC AGCCCGGCGC CTCGAAGAGC
    CCACCCGAGG CCGCCAGGGC CCTCGCCAGC CCGAAGAGCG AGGCAGCCTC CGTCAGCACC
    ACAAACCCTG CCCAGGGCGA GGACTTTAAA ATGGACGAAG GGAACTTCAA GACCCCAGAT
    ATTGACCTTG CCAAGGATGT TTTTGCAGCC CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC
    AGTGGACAAG CCTCTGAGCT TGCTCCTTCC ACGGCCGACG GCTCTCTTCC GCCTGCACCA
    GTCAAGACCG AGAAGGGTCC CGTAGAAGCA AAGCCGGAGA CAGAAACGAA GCCAGCGCCA
    GCCGAAGTCC AGACGGTCCC CAACGATGCC ACACAAATAA AGGAGAATGA GAGCAAAGCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810649.2
    CDS291..3653
    Translation

    Target ORF information:

    RefSeq Version XM_010812347.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812347.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACGGAGCC GAACGAGACC ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT
    GACACCACAG CCACTGTGGA GGACATGCTG CCTTCTGTCA CCACCGTCAC CGCTAACTCT
    GACACTATCA CCGAAACCTT TGCCACTGCT CAGAACAGCC CCACCAGTGA GACCACCACC
    CTGACCTCCA GTATCGCCCC CCCGGTCGCG GCCACCCCTG ATTCAAACTC TGTGCCCGCC
    GGCCAGGCCA CCCCGTCCAA GGGGACCGGC GTCACTGCCG CCTCCCCGGC CCCGGCCCCA
    GCCCCCAAGG TCGCCCCCCT CGTTGACCTG AGCGACCCTC CGGCCTCAGC CCCGGCCGCC
    AACAACCTGT CCTCTAGCGT CCTGGCCAAC CAGGGGGCCG TGCTCAGCCC CAGCACCCCG
    GCCAGCGCGG GCGAGGCCCC CAAGACCCCT CCAGCCAGCA AGGCAGTCTC CCCGTCAGCA
    GCCAGTCCCC TGGCAGCAGC CCCGACAGCC CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG
    GAGGCTCCCG GCACCAAAGG TCCGGACCCC GAGCCCACCC AGCCCGGCGC CTCGAAGAGC
    CCACCCGAGG CCGCCAGGGC CCTCGCCAGC CCGAAGAGCG AGGCAGCCTC CGTCAGCACC
    ACAAACCCTG CCCAGGGCGA GGACTTTAAA ATGGACGAAG GGAACTTCAA GACCCCAGAT
    ATTGACCTTG CCAAGGATGT TTTTGCAGCC CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC
    AGTGGACAAG CCTCTGAGCT TGCTCCTTCC ACGGCCGACG GCTCTCTTCC GCCTGCACCA
    GTCAAGACCG AGAAGGGTCC CGTAGAAGCA AAGCCGGAGA CAGAAACGAA GCCAGCGCCA
    GCCGAAGTCC AGACGGTCCC CAACGATGCC ACACAAATAA AGGAGAATGA GAGCAAAGCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222636
    Clone ID Related Accession (Same CDS sequence) XM_024974999.1
    Accession Version XM_024974999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    AAGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
    CTCAAGTCCC TGGACTGGAA TGCCGAGTAC GAGGTCTATG TGGTGGCCGA GAACCAGCAG
    GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
    GCCAACGGCA GCCCCACCTC GGGCCTGAGC ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG
    ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC GTCACCTGCT ACTTCCTGAA CAAGTGTGGC
    CTGCTCATGT GCATTGCCGT CAACCTGTGT GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG
    GACATGGAGG AGGGCAAAGC GGCCTTCTCG AAAGATGAGT CCAAGGAGCC TATCGTGGAG
    GTGCGAACGG AGGAGGAGCG GACCCCGAAT CACGACGGAG GGAAACACAC GGAGCCGAAC
    GAGACCACGC CGCTGACGGA GCCCGAGCTG CCTGCTGACA CCACAGCCAC TGTGGAGGAC
    ATGCTGCCTT CTGTCACCAC CGTCACCGCT AACTCTGACA CTATCACCGA AACCTTTGCC
    ACTGCTCAGA ACAGCCCCAC CAGTGAGACC ACCACCCTGA CCTCCAGTAT CGCCCCCCCG
    GTCGCGGCCA CCCCTGATTC AAACTCTGTG CCCGCCGGCC AGGCCACCCC GTCCAAGGGG
    ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT
    GACCTGAGCG ACCCTCCGGC CTCAGCCCCG GCCGCCAACA ACCTGTCCTC TAGCGTCCTG
    GCCAACCAGG GGGCCGTGCT CAGCCCCAGC ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG
    ACCCCTCCAG CCAGCAAGGC AGTCTCCCCG TCAGCAGCCA GTCCCCTGGC AGCAGCCCCG
    ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG
    GACCCCGAGC CCACCCAGCC CGGCGCCTCG AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC
    GCCAGCCCGA AGAGCGAGGC AGCCTCCGTC AGCACCACAA ACCCTGCCCA GGGCGAGGAC
    TTTAAAATGG ACGAAGGGAA CTTCAAGACC CCAGATATTG ACCTTGCCAA GGATGTTTTT
    GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT GGGGCCAGTG GACAAGCCTC TGAGCTTGCT
    CCTTCCACGG CCGACGGCTC TCTTCCGCCT GCACCAGTCA AGACCGAGAA GGGTCCCGTA
    GAAGCAAAGC CGGAGACAGA AACGAAGCCA GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC
    GATGCCACAC AAATAAAGGA GAATGAGAGC AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830767.1
    CDS291..3629
    Translation

    Target ORF information:

    RefSeq Version XM_024974999.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024974999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA
    CCCAGTGCAC CTAAGCTCGA AGGGCAGATG GGAGAGGATG GAAACTCTAT TAAGGTGAAG
    CTGATCAAGC AAGACGACGG CGGCTCCCCC ATCCGACACT ACCTGGTCAA ATACCGAGCG
    AAGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
    CTCAAGTCCC TGGACTGGAA TGCCGAGTAC GAGGTCTATG TGGTGGCCGA GAACCAGCAG
    GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
    GCCAACGGCA GCCCCACCTC GGGCCTGAGC ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG
    ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC GTCACCTGCT ACTTCCTGAA CAAGTGTGGC
    CTGCTCATGT GCATTGCCGT CAACCTGTGT GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG
    GACATGGAGG AGGGCAAAGC GGCCTTCTCG AAAGATGAGT CCAAGGAGCC TATCGTGGAG
    GTGCGAACGG AGGAGGAGCG GACCCCGAAT CACGACGGAG GGAAACACAC GGAGCCGAAC
    GAGACCACGC CGCTGACGGA GCCCGAGCTG CCTGCTGACA CCACAGCCAC TGTGGAGGAC
    ATGCTGCCTT CTGTCACCAC CGTCACCGCT AACTCTGACA CTATCACCGA AACCTTTGCC
    ACTGCTCAGA ACAGCCCCAC CAGTGAGACC ACCACCCTGA CCTCCAGTAT CGCCCCCCCG
    GTCGCGGCCA CCCCTGATTC AAACTCTGTG CCCGCCGGCC AGGCCACCCC GTCCAAGGGG
    ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT
    GACCTGAGCG ACCCTCCGGC CTCAGCCCCG GCCGCCAACA ACCTGTCCTC TAGCGTCCTG
    GCCAACCAGG GGGCCGTGCT CAGCCCCAGC ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG
    ACCCCTCCAG CCAGCAAGGC AGTCTCCCCG TCAGCAGCCA GTCCCCTGGC AGCAGCCCCG
    ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG
    GACCCCGAGC CCACCCAGCC CGGCGCCTCG AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC
    GCCAGCCCGA AGAGCGAGGC AGCCTCCGTC AGCACCACAA ACCCTGCCCA GGGCGAGGAC
    TTTAAAATGG ACGAAGGGAA CTTCAAGACC CCAGATATTG ACCTTGCCAA GGATGTTTTT
    GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT GGGGCCAGTG GACAAGCCTC TGAGCTTGCT
    CCTTCCACGG CCGACGGCTC TCTTCCGCCT GCACCAGTCA AGACCGAGAA GGGTCCCGTA
    GAAGCAAAGC CGGAGACAGA AACGAAGCCA GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC
    GATGCCACAC AAATAAAGGA GAATGAGAGC AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222637
    Clone ID Related Accession (Same CDS sequence) XM_024975000.1
    Accession Version XM_024975000.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAAGCG GCGCACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACGGAGC CGAACGAGAC CACGCCGCTG ACGGAGCCCG AGCTGCCTGC TGACACCACA
    GCCACTGTGG AGGACATGCT GCCTTCTGTC ACCACCGTCA CCGCTAACTC TGACACTATC
    ACCGAAACCT TTGCCACTGC TCAGAACAGC CCCACCAGTG AGACCACCAC CCTGACCTCC
    AGTATCGCCC CCCCGGTCGC GGCCACCCCT GATTCAAACT CTGTGCCCGC CGGCCAGGCC
    ACCCCGTCCA AGGGGACCGG CGTCACTGCC GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG
    GTCGCCCCCC TCGTTGACCT GAGCGACCCT CCGGCCTCAG CCCCGGCCGC CAACAACCTG
    TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC GTGCTCAGCC CCAGCACCCC GGCCAGCGCG
    GGCGAGGCCC CCAAGACCCC TCCAGCCAGC AAGGCAGTCT CCCCGTCAGC AGCCAGTCCC
    CTGGCAGCAG CCCCGACAGC CCCTGCCCCG GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC
    GGCACCAAAG GTCCGGACCC CGAGCCCACC CAGCCCGGCG CCTCGAAGAG CCCACCCGAG
    GCCGCCAGGG CCCTCGCCAG CCCGAAGAGC GAGGCAGCCT CCGTCAGCAC CACAAACCCT
    GCCCAGGGCG AGGACTTTAA AATGGACGAA GGGAACTTCA AGACCCCAGA TATTGACCTT
    GCCAAGGATG TTTTTGCAGC CCTGGGCTCT CCTGCTCCCG CCGCTGGGGC CAGTGGACAA
    GCCTCTGAGC TTGCTCCTTC CACGGCCGAC GGCTCTCTTC CGCCTGCACC AGTCAAGACC
    GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG ACAGAAACGA AGCCAGCGCC AGCCGAAGTC
    CAGACGGTCC CCAACGATGC CACACAAATA AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024830768.1
    CDS302..3775
    Translation

    Target ORF information:

    RefSeq Version XM_024975000.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATACAACC TGGCCTCCTC CTGCTCTTAC AACCACCGAA
    GCAACTAAAG GGGAACCCAG TGCACCTAAG CTCGAAGGGC AGATGGGAGA GGATGGAAAC
    TCTATTAAGG TGAAGCTGAT CAAGCAAGAC GACGGCGGCT CCCCCATCCG ACACTACCTG
    GTCAAATACC GAGCGAAGCT CTCCTCCGAG TGGAAACCAG AGATCAGGCT CCCGTCTGGC
    AGTGACCACG TCATGCTCAA GTCCCTGGAC TGGAATGCCG AGTACGAGGT CTATGTGGTG
    GCCGAGAACC AGCAGGGCAA GTCCAAAGCG GCGCACTTTG TGTTCAGGAC CTCGGCCCAG
    CCCACAGCCA TCCCAGCCAA CGGCAGCCCC ACCTCGGGCC TGAGCACTGG CGCCATCGTG
    GGCATCCTCG TCGTGACCTT CGTCCTGCTC CTGGTGGCCG TGGACGTCAC CTGCTACTTC
    CTGAACAAGT GTGGCCTGCT CATGTGCATT GCCGTCAACC TGTGTGGCAA AGCCGGGCCC
    GGAGCCAAGG GCAAGGACAT GGAGGAGGGC AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG
    GAGCCTATCG TGGAGGTGCG AACGGAGGAG GAGCGGACCC CGAATCACGA CGGAGGGAAA
    CACACGGAGC CGAACGAGAC CACGCCGCTG ACGGAGCCCG AGCTGCCTGC TGACACCACA
    GCCACTGTGG AGGACATGCT GCCTTCTGTC ACCACCGTCA CCGCTAACTC TGACACTATC
    ACCGAAACCT TTGCCACTGC TCAGAACAGC CCCACCAGTG AGACCACCAC CCTGACCTCC
    AGTATCGCCC CCCCGGTCGC GGCCACCCCT GATTCAAACT CTGTGCCCGC CGGCCAGGCC
    ACCCCGTCCA AGGGGACCGG CGTCACTGCC GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG
    GTCGCCCCCC TCGTTGACCT GAGCGACCCT CCGGCCTCAG CCCCGGCCGC CAACAACCTG
    TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC GTGCTCAGCC CCAGCACCCC GGCCAGCGCG
    GGCGAGGCCC CCAAGACCCC TCCAGCCAGC AAGGCAGTCT CCCCGTCAGC AGCCAGTCCC
    CTGGCAGCAG CCCCGACAGC CCCTGCCCCG GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC
    GGCACCAAAG GTCCGGACCC CGAGCCCACC CAGCCCGGCG CCTCGAAGAG CCCACCCGAG
    GCCGCCAGGG CCCTCGCCAG CCCGAAGAGC GAGGCAGCCT CCGTCAGCAC CACAAACCCT
    GCCCAGGGCG AGGACTTTAA AATGGACGAA GGGAACTTCA AGACCCCAGA TATTGACCTT
    GCCAAGGATG TTTTTGCAGC CCTGGGCTCT CCTGCTCCCG CCGCTGGGGC CAGTGGACAA
    GCCTCTGAGC TTGCTCCTTC CACGGCCGAC GGCTCTCTTC CGCCTGCACC AGTCAAGACC
    GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG ACAGAAACGA AGCCAGCGCC AGCCGAAGTC
    CAGACGGTCC CCAACGATGC CACACAAATA AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222651
    Clone ID Related Accession (Same CDS sequence) XM_010812356.3
    Accession Version XM_010812356.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT GACACCACAG CCACTGTGGA GGACATGCTG
    CCTTCTGTCA CCACCGTCAC CGCTAACTCT GACACTATCA CCGAAACCTT TGCCACTGCT
    CAGAACAGCC CCACCAGTGA GACCACCACC CTGACCTCCA GTATCGCCCC CCCGGTCGCG
    GCCACCCCTG ATTCAAACTC TGTGCCCGCC GGCCAGGCCA CCCCGTCCAA GGGGACCGGC
    GTCACTGCCG CCTCCCCGGC CCCGGCCCCA GCCCCCAAGG TCGCCCCCCT CGTTGACCTG
    AGCGACCCTC CGGCCTCAGC CCCGGCCGCC AACAACCTGT CCTCTAGCGT CCTGGCCAAC
    CAGGGGGCCG TGCTCAGCCC CAGCACCCCG GCCAGCGCGG GCGAGGCCCC CAAGACCCCT
    CCAGCCAGCA AGGCAGTCTC CCCGTCAGCA GCCAGTCCCC TGGCAGCAGC CCCGACAGCC
    CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG GAGGCTCCCG GCACCAAAGG TCCGGACCCC
    GAGCCCACCC AGCCCGGCGC CTCGAAGAGC CCACCCGAGG CCGCCAGGGC CCTCGCCAGC
    CCGAAGAGCG AGGCAGCCTC CGTCAGCACC ACAAACCCTG CCCAGGGCGA GGACTTTAAA
    ATGGACGAAG GGAACTTCAA GACCCCAGAT ATTGACCTTG CCAAGGATGT TTTTGCAGCC
    CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC AGTGGACAAG CCTCTGAGCT TGCTCCTTCC
    ACGGCCGACG GCTCTCTTCC GCCTGCACCA GTCAAGACCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACGAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810658.2
    CDS291..3623
    Translation

    Target ORF information:

    RefSeq Version XM_010812356.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812356.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT GACACCACAG CCACTGTGGA GGACATGCTG
    CCTTCTGTCA CCACCGTCAC CGCTAACTCT GACACTATCA CCGAAACCTT TGCCACTGCT
    CAGAACAGCC CCACCAGTGA GACCACCACC CTGACCTCCA GTATCGCCCC CCCGGTCGCG
    GCCACCCCTG ATTCAAACTC TGTGCCCGCC GGCCAGGCCA CCCCGTCCAA GGGGACCGGC
    GTCACTGCCG CCTCCCCGGC CCCGGCCCCA GCCCCCAAGG TCGCCCCCCT CGTTGACCTG
    AGCGACCCTC CGGCCTCAGC CCCGGCCGCC AACAACCTGT CCTCTAGCGT CCTGGCCAAC
    CAGGGGGCCG TGCTCAGCCC CAGCACCCCG GCCAGCGCGG GCGAGGCCCC CAAGACCCCT
    CCAGCCAGCA AGGCAGTCTC CCCGTCAGCA GCCAGTCCCC TGGCAGCAGC CCCGACAGCC
    CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG GAGGCTCCCG GCACCAAAGG TCCGGACCCC
    GAGCCCACCC AGCCCGGCGC CTCGAAGAGC CCACCCGAGG CCGCCAGGGC CCTCGCCAGC
    CCGAAGAGCG AGGCAGCCTC CGTCAGCACC ACAAACCCTG CCCAGGGCGA GGACTTTAAA
    ATGGACGAAG GGAACTTCAA GACCCCAGAT ATTGACCTTG CCAAGGATGT TTTTGCAGCC
    CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC AGTGGACAAG CCTCTGAGCT TGCTCCTTCC
    ACGGCCGACG GCTCTCTTCC GCCTGCACCA GTCAAGACCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACGAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45263
    Clone ID Related Accession (Same CDS sequence) XM_010812339.2
    Accession Version XM_010812339.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812339.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCGGCCTTC
    TCGAAAGATG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA GCGGACCCCG
    AATCACGACG GAGGGAAACA CACGGAGCCG AACGAGACCA CGCCGCTGAC GGAGCCCGAG
    CTGCCTGCTG ACACCACAGC CACTGTGGAG GACATGCTGC CTTCTGTCAC CACCGTCACC
    GCTAACTCTG ACACTATCAC CGAAACCTTT GCCACTGCTC AGAACAGCCC CACCAGTGAG
    ACCACCACCC TGACCTCCAG TATCGCCCCC CCGGTCGCGG CCACCCCTGA TTCAAACTCT
    GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG GGGACCGGCG TCACTGCCGC CTCCCCGGCC
    CCGGCCCCAG CCCCCAAGGT CGCCCCCCTC GTTGACCTGA GCGACCCTCC GGCCTCAGCC
    CCGGCCGCCA ACAACCTGTC CTCTAGCGTC CTGGCCAACC AGGGGGCCGT GCTCAGCCCC
    AGCACCCCGG CCAGCGCGGG CGAGGCCCCC AAGGCCCCTC CAGCCAGCAA GGCAGTCTCC
    CCGTCGGCAG CCAGTCCCCT GGCAGCAGCC CCGACAGCCC CTGCCCCGGA AGCCCCCCAG
    GCCAAGCAGG AGGCTCCCGG CACCAAAGGT CCGGACCCCG AGCCCACCCA GCCCGGCGCC
    TCGAAGAGCC CACCCGAGGC CGCCAGGGCC CTCGCCAGCC CGAAGAGCGA GGCGGCCTCC
    GTCAGCACCA CAAACCCTGC CCAGGGCGAG GACTTTAAAA TGGACGAAGG GAACTTCAAG
    ACCCCAGATA TTGACCTTGC CAAGGATGTT TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC
    GCTGGGGCCA GTGGACAAGC CTCTGAGCTT GCTCCTTCCA CGGCCGACGG CTCTCTTCCG
    CCTGCACCAG TCAAGACCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810641.1
    CDS304..3735
    Translation

    Target ORF information:

    RefSeq Version XM_010812339.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812339.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGAAGCTCT CCTCCGAGTG GAAACCAGAG
    ATCAGGCTCC CGTCTGGCAG TGACCACGTC ATGCTCAAGT CCCTGGACTG GAATGCCGAG
    TACGAGGTCT ATGTGGTGGC CGAGAACCAG CAGGGCAAGT CCAAAGCGGC GCACTTTGTG
    TTCAGGACCT CGGCCCAGCC CACAGCCATC CCAGCCAACG GCAGCCCCAC CTCGGGCCTG
    AGCACTGGCG CCATCGTGGG CATCCTCGTC GTGACCTTCG TCCTGCTCCT GGTGGCCGTG
    GACGTCACCT GCTACTTCCT GAACAAGTGT GGCCTGCTCA TGTGCATTGC CGTCAACCTG
    TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC AAGGACATGG AGGAGGGCAA AGCGGCCTTC
    TCGAAAGATG AGTCCAAGGA GCCTATCGTG GAGGTGCGAA CGGAGGAGGA GCGGACCCCG
    AATCACGACG GAGGGAAACA CACGGAGCCG AACGAGACCA CGCCGCTGAC GGAGCCCGAG
    CTGCCTGCTG ACACCACAGC CACTGTGGAG GACATGCTGC CTTCTGTCAC CACCGTCACC
    GCTAACTCTG ACACTATCAC CGAAACCTTT GCCACTGCTC AGAACAGCCC CACCAGTGAG
    ACCACCACCC TGACCTCCAG TATCGCCCCC CCGGTCGCGG CCACCCCTGA TTCAAACTCT
    GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG GGGACCGGCG TCACTGCCGC CTCCCCGGCC
    CCGGCCCCAG CCCCCAAGGT CGCCCCCCTC GTTGACCTGA GCGACCCTCC GGCCTCAGCC
    CCGGCCGCCA ACAACCTGTC CTCTAGCGTC CTGGCCAACC AGGGGGCCGT GCTCAGCCCC
    AGCACCCCGG CCAGCGCGGG CGAGGCCCCC AAGGCCCCTC CAGCCAGCAA GGCAGTCTCC
    CCGTCGGCAG CCAGTCCCCT GGCAGCAGCC CCGACAGCCC CTGCCCCGGA AGCCCCCCAG
    GCCAAGCAGG AGGCTCCCGG CACCAAAGGT CCGGACCCCG AGCCCACCCA GCCCGGCGCC
    TCGAAGAGCC CACCCGAGGC CGCCAGGGCC CTCGCCAGCC CGAAGAGCGA GGCGGCCTCC
    GTCAGCACCA CAAACCCTGC CCAGGGCGAG GACTTTAAAA TGGACGAAGG GAACTTCAAG
    ACCCCAGATA TTGACCTTGC CAAGGATGTT TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC
    GCTGGGGCCA GTGGACAAGC CTCTGAGCTT GCTCCTTCCA CGGCCGACGG CTCTCTTCCG
    CCTGCACCAG TCAAGACCGA GAAGGGTCCC GTAGAAGCAA AGCCGGAGAC AGAAACGAAG
    CCAGCGCCAG CCGAAGTCCA GACGGTCCCC AACGATGCCA CACAAATAAA GGAGAATGAG
    AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45264
    Clone ID Related Accession (Same CDS sequence) XM_010812340.2
    Accession Version XM_010812340.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCGGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810642.1
    CDS304..3732
    Translation

    Target ORF information:

    RefSeq Version XM_010812340.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812340.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
    ATGGGAGAGG ATGGAAACTC TATTAAGGTG AAGCTGATCA AGCAAGACGA CGGCGGCTCC
    CCCATCCGAC ACTACCTGGT CAAATACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCGGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45265
    Clone ID Related Accession (Same CDS sequence) XM_010812341.2
    Accession Version XM_010812341.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCGGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810643.1
    CDS304..3732
    Translation

    Target ORF information:

    RefSeq Version XM_010812341.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812341.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACCGG CTCCTGCTAG CTCTTCCACC
    CCTGTTCCGT TGTCTCATCT GGATAGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCGGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45266
    Clone ID Related Accession (Same CDS sequence) XM_010812342.2
    Accession Version XM_010812342.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATAAAGGG
    GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAGGTG
    AAGCTGATCA AGCAAGACGA CGGCGGCTCC CCCATCCGAC ACTACCTGGT CAAATACCGA
    GCGAAGCTCT CCTCCGAGTG GAAACCAGAG ATCAGGCTCC CGTCTGGCAG TGACCACGTC
    ATGCTCAAGT CCCTGGACTG GAATGCCGAG TACGAGGTCT ATGTGGTGGC CGAGAACCAG
    CAGGGCAAGT CCAAAGCGGC GCACTTTGTG TTCAGGACCT CGGCCCAGCC CACAGCCATC
    CCAGCCAACG GCAGCCCCAC CTCGGGCCTG AGCACTGGCG CCATCGTGGG CATCCTCGTC
    GTGACCTTCG TCCTGCTCCT GGTGGCCGTG GACGTCACCT GCTACTTCCT GAACAAGTGT
    GGCCTGCTCA TGTGCATTGC CGTCAACCTG TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC
    AAGGACATGG AGGAGGGCAA AGCGGCCTTC TCGAAAGATG AGTCCAAGGA GCCTATCGTG
    GAGGTGCGAA CGGAGGAGGA GCGGACCCCG AATCACGACG GAGGGAAACA CACGGAGCCG
    AACGAGACCA CGCCGCTGAC GGAGCCCGAG CTGCCTGCTG ACACCACAGC CACTGTGGAG
    GACATGCTGC CTTCTGTCAC CACCGTCACC GCTAACTCTG ACACTATCAC CGAAACCTTT
    GCCACTGCTC AGAACAGCCC CACCAGTGAG ACCACCACCC TGACCTCCAG TATCGCCCCC
    CCGGTCGCGG CCACCCCTGA TTCAAACTCT GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG
    GGGACCGGCG TCACTGCCGC CTCCCCGGCC CCGGCCCCAG CCCCCAAGGT CGCCCCCCTC
    GTTGACCTGA GCGACCCTCC GGCCTCAGCC CCGGCCGCCA ACAACCTGTC CTCTAGCGTC
    CTGGCCAACC AGGGGGCCGT GCTCAGCCCC AGCACCCCGG CCAGCGCGGG CGAGGCCCCC
    AAGGCCCCTC CAGCCAGCAA GGCAGTCTCC CCGTCGGCAG CCAGTCCCCT GGCAGCAGCC
    CCGACAGCCC CTGCCCCGGA AGCCCCCCAG GCCAAGCAGG AGGCTCCCGG CACCAAAGGT
    CCGGACCCCG AGCCCACCCA GCCCGGCGCC TCGAAGAGCC CACCCGAGGC CGCCAGGGCC
    CTCGCCAGCC CGAAGAGCGA GGCGGCCTCC GTCAGCACCA CAAACCCTGC CCAGGGCGAG
    GACTTTAAAA TGGACGAAGG GAACTTCAAG ACCCCAGATA TTGACCTTGC CAAGGATGTT
    TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC GCTGGGGCCA GTGGACAAGC CTCTGAGCTT
    GCTCCTTCCA CGGCCGACGG CTCTCTTCCG CCTGCACCAG TCAAGACCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACGAAG CCAGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGAGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810644.1
    CDS304..3705
    Translation

    Target ORF information:

    RefSeq Version XM_010812342.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812342.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCATAGCCCT
    CTTCCACCGG CTCCTGCTAG CTCTTCCACC CCTGTTCCGT TGTCTCATCT GGATAAAGGG
    GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAGGTG
    AAGCTGATCA AGCAAGACGA CGGCGGCTCC CCCATCCGAC ACTACCTGGT CAAATACCGA
    GCGAAGCTCT CCTCCGAGTG GAAACCAGAG ATCAGGCTCC CGTCTGGCAG TGACCACGTC
    ATGCTCAAGT CCCTGGACTG GAATGCCGAG TACGAGGTCT ATGTGGTGGC CGAGAACCAG
    CAGGGCAAGT CCAAAGCGGC GCACTTTGTG TTCAGGACCT CGGCCCAGCC CACAGCCATC
    CCAGCCAACG GCAGCCCCAC CTCGGGCCTG AGCACTGGCG CCATCGTGGG CATCCTCGTC
    GTGACCTTCG TCCTGCTCCT GGTGGCCGTG GACGTCACCT GCTACTTCCT GAACAAGTGT
    GGCCTGCTCA TGTGCATTGC CGTCAACCTG TGTGGCAAAG CCGGGCCCGG AGCCAAGGGC
    AAGGACATGG AGGAGGGCAA AGCGGCCTTC TCGAAAGATG AGTCCAAGGA GCCTATCGTG
    GAGGTGCGAA CGGAGGAGGA GCGGACCCCG AATCACGACG GAGGGAAACA CACGGAGCCG
    AACGAGACCA CGCCGCTGAC GGAGCCCGAG CTGCCTGCTG ACACCACAGC CACTGTGGAG
    GACATGCTGC CTTCTGTCAC CACCGTCACC GCTAACTCTG ACACTATCAC CGAAACCTTT
    GCCACTGCTC AGAACAGCCC CACCAGTGAG ACCACCACCC TGACCTCCAG TATCGCCCCC
    CCGGTCGCGG CCACCCCTGA TTCAAACTCT GTGCCCGCCG GCCAGGCCAC CCCGTCCAAG
    GGGACCGGCG TCACTGCCGC CTCCCCGGCC CCGGCCCCAG CCCCCAAGGT CGCCCCCCTC
    GTTGACCTGA GCGACCCTCC GGCCTCAGCC CCGGCCGCCA ACAACCTGTC CTCTAGCGTC
    CTGGCCAACC AGGGGGCCGT GCTCAGCCCC AGCACCCCGG CCAGCGCGGG CGAGGCCCCC
    AAGGCCCCTC CAGCCAGCAA GGCAGTCTCC CCGTCGGCAG CCAGTCCCCT GGCAGCAGCC
    CCGACAGCCC CTGCCCCGGA AGCCCCCCAG GCCAAGCAGG AGGCTCCCGG CACCAAAGGT
    CCGGACCCCG AGCCCACCCA GCCCGGCGCC TCGAAGAGCC CACCCGAGGC CGCCAGGGCC
    CTCGCCAGCC CGAAGAGCGA GGCGGCCTCC GTCAGCACCA CAAACCCTGC CCAGGGCGAG
    GACTTTAAAA TGGACGAAGG GAACTTCAAG ACCCCAGATA TTGACCTTGC CAAGGATGTT
    TTTGCAGCCC TGGGCTCTCC TGCTCCCGCC GCTGGGGCCA GTGGACAAGC CTCTGAGCTT
    GCTCCTTCCA CGGCCGACGG CTCTCTTCCG CCTGCACCAG TCAAGACCGA GAAGGGTCCC
    GTAGAAGCAA AGCCGGAGAC AGAAACGAAG CCAGCGCCAG CCGAAGTCCA GACGGTCCCC
    AACGATGCCA CACAAATAAA GGAGAATGAG AGCAAAGCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45267
    Clone ID Related Accession (Same CDS sequence) XM_010812343.2
    Accession Version XM_010812343.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAAGCG
    GCGCACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAGCGGACCC CGAATCACGA CGGAGGGAAA CACACGGAGC CGAACGAGAC CACGCCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GATTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTCGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCAGTCT CCCCGTCGGC AGCCAGTCCC CTGGCAGCAG CCCCGACAGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCAGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGTCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACGA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810645.1
    CDS304..3687
    Translation

    Target ORF information:

    RefSeq Version XM_010812343.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812343.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACAAG GGGAACCCAG TGCACCTAAG
    CTCGAAGGGC AGATGGGAGA GGATGGAAAC TCTATTAAGG TGAAGCTGAT CAAGCAAGAC
    GACGGCGGCT CCCCCATCCG ACACTACCTG GTCAAATACC GAGCGAAGCT CTCCTCCGAG
    TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTCAA GTCCCTGGAC
    TGGAATGCCG AGTACGAGGT CTATGTGGTG GCCGAGAACC AGCAGGGCAA GTCCAAAGCG
    GCGCACTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCCAA CGGCAGCCCC
    ACCTCGGGCC TGAGCACTGG CGCCATCGTG GGCATCCTCG TCGTGACCTT CGTCCTGCTC
    CTGGTGGCCG TGGACGTCAC CTGCTACTTC CTGAACAAGT GTGGCCTGCT CATGTGCATT
    GCCGTCAACC TGTGTGGCAA AGCCGGGCCC GGAGCCAAGG GCAAGGACAT GGAGGAGGGC
    AAAGCGGCCT TCTCGAAAGA TGAGTCCAAG GAGCCTATCG TGGAGGTGCG AACGGAGGAG
    GAGCGGACCC CGAATCACGA CGGAGGGAAA CACACGGAGC CGAACGAGAC CACGCCGCTG
    ACGGAGCCCG AGCTGCCTGC TGACACCACA GCCACTGTGG AGGACATGCT GCCTTCTGTC
    ACCACCGTCA CCGCTAACTC TGACACTATC ACCGAAACCT TTGCCACTGC TCAGAACAGC
    CCCACCAGTG AGACCACCAC CCTGACCTCC AGTATCGCCC CCCCGGTCGC GGCCACCCCT
    GATTCAAACT CTGTGCCCGC CGGCCAGGCC ACCCCGTCCA AGGGGACCGG CGTCACTGCC
    GCCTCCCCGG CCCCGGCCCC AGCCCCCAAG GTCGCCCCCC TCGTTGACCT GAGCGACCCT
    CCGGCCTCAG CCCCGGCCGC CAACAACCTG TCCTCTAGCG TCCTGGCCAA CCAGGGGGCC
    GTGCTCAGCC CCAGCACCCC GGCCAGCGCG GGCGAGGCCC CCAAGGCCCC TCCAGCCAGC
    AAGGCAGTCT CCCCGTCGGC AGCCAGTCCC CTGGCAGCAG CCCCGACAGC CCCTGCCCCG
    GAAGCCCCCC AGGCCAAGCA GGAGGCTCCC GGCACCAAAG GTCCGGACCC CGAGCCCACC
    CAGCCCGGCG CCTCGAAGAG CCCACCCGAG GCCGCCAGGG CCCTCGCCAG CCCGAAGAGC
    GAGGCGGCCT CCGTCAGCAC CACAAACCCT GCCCAGGGCG AGGACTTTAA AATGGACGAA
    GGGAACTTCA AGACCCCAGA TATTGACCTT GCCAAGGATG TTTTTGCAGC CCTGGGCTCT
    CCTGCTCCCG CCGCTGGGGC CAGTGGACAA GCCTCTGAGC TTGCTCCTTC CACGGCCGAC
    GGCTCTCTTC CGCCTGCACC AGTCAAGACC GAGAAGGGTC CCGTAGAAGC AAAGCCGGAG
    ACAGAAACGA AGCCAGCGCC AGCCGAAGTC CAGACGGTCC CCAACGATGC CACACAAATA
    AAGGAGAATG AGAGCAAAGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45268
    Clone ID Related Accession (Same CDS sequence) XM_010812344.2
    Accession Version XM_010812344.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCGGCCTTCT CGAAAGATGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAG
    CGGACCCCGA ATCACGACGG AGGGAAACAC ACGGAGCCGA ACGAGACCAC GCCGCTGACG
    GAGCCCGAGC TGCCTGCTGA CACCACAGCC ACTGTGGAGG ACATGCTGCC TTCTGTCACC
    ACCGTCACCG CTAACTCTGA CACTATCACC GAAACCTTTG CCACTGCTCA GAACAGCCCC
    ACCAGTGAGA CCACCACCCT GACCTCCAGT ATCGCCCCCC CGGTCGCGGC CACCCCTGAT
    TCAAACTCTG TGCCCGCCGG CCAGGCCACC CCGTCCAAGG GGACCGGCGT CACTGCCGCC
    TCCCCGGCCC CGGCCCCAGC CCCCAAGGTC GCCCCCCTCG TTGACCTGAG CGACCCTCCG
    GCCTCAGCCC CGGCCGCCAA CAACCTGTCC TCTAGCGTCC TGGCCAACCA GGGGGCCGTG
    CTCAGCCCCA GCACCCCGGC CAGCGCGGGC GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG
    GCAGTCTCCC CGTCGGCAGC CAGTCCCCTG GCAGCAGCCC CGACAGCCCC TGCCCCGGAA
    GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC ACCAAAGGTC CGGACCCCGA GCCCACCCAG
    CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC GCCAGGGCCC TCGCCAGCCC GAAGAGCGAG
    GCGGCCTCCG TCAGCACCAC AAACCCTGCC CAGGGCGAGG ACTTTAAAAT GGACGAAGGG
    AACTTCAAGA CCCCAGATAT TGACCTTGCC AAGGATGTTT TTGCAGCCCT GGGCTCTCCT
    GCTCCCGCCG CTGGGGCCAG TGGACAAGCC TCTGAGCTTG CTCCTTCCAC GGCCGACGGC
    TCTCTTCCGC CTGCACCAGT CAAGACCGAG AAGGGTCCCG TAGAAGCAAA GCCGGAGACA
    GAAACGAAGC CAGCGCCAGC CGAAGTCCAG ACGGTCCCCA ACGATGCCAC ACAAATAAAG
    GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810646.1
    CDS304..3684
    Translation

    Target ORF information:

    RefSeq Version XM_010812344.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812344.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCATAGCCCT CTTCCACGGG AACCCAGTGC ACCTAAGCTC
    GAAGGGCAGA TGGGAGAGGA TGGAAACTCT ATTAAGGTGA AGCTGATCAA GCAAGACGAC
    GGCGGCTCCC CCATCCGACA CTACCTGGTC AAATACCGAG CGAAGCTCTC CTCCGAGTGG
    AAACCAGAGA TCAGGCTCCC GTCTGGCAGT GACCACGTCA TGCTCAAGTC CCTGGACTGG
    AATGCCGAGT ACGAGGTCTA TGTGGTGGCC GAGAACCAGC AGGGCAAGTC CAAAGCGGCG
    CACTTTGTGT TCAGGACCTC GGCCCAGCCC ACAGCCATCC CAGCCAACGG CAGCCCCACC
    TCGGGCCTGA GCACTGGCGC CATCGTGGGC ATCCTCGTCG TGACCTTCGT CCTGCTCCTG
    GTGGCCGTGG ACGTCACCTG CTACTTCCTG AACAAGTGTG GCCTGCTCAT GTGCATTGCC
    GTCAACCTGT GTGGCAAAGC CGGGCCCGGA GCCAAGGGCA AGGACATGGA GGAGGGCAAA
    GCGGCCTTCT CGAAAGATGA GTCCAAGGAG CCTATCGTGG AGGTGCGAAC GGAGGAGGAG
    CGGACCCCGA ATCACGACGG AGGGAAACAC ACGGAGCCGA ACGAGACCAC GCCGCTGACG
    GAGCCCGAGC TGCCTGCTGA CACCACAGCC ACTGTGGAGG ACATGCTGCC TTCTGTCACC
    ACCGTCACCG CTAACTCTGA CACTATCACC GAAACCTTTG CCACTGCTCA GAACAGCCCC
    ACCAGTGAGA CCACCACCCT GACCTCCAGT ATCGCCCCCC CGGTCGCGGC CACCCCTGAT
    TCAAACTCTG TGCCCGCCGG CCAGGCCACC CCGTCCAAGG GGACCGGCGT CACTGCCGCC
    TCCCCGGCCC CGGCCCCAGC CCCCAAGGTC GCCCCCCTCG TTGACCTGAG CGACCCTCCG
    GCCTCAGCCC CGGCCGCCAA CAACCTGTCC TCTAGCGTCC TGGCCAACCA GGGGGCCGTG
    CTCAGCCCCA GCACCCCGGC CAGCGCGGGC GAGGCCCCCA AGGCCCCTCC AGCCAGCAAG
    GCAGTCTCCC CGTCGGCAGC CAGTCCCCTG GCAGCAGCCC CGACAGCCCC TGCCCCGGAA
    GCCCCCCAGG CCAAGCAGGA GGCTCCCGGC ACCAAAGGTC CGGACCCCGA GCCCACCCAG
    CCCGGCGCCT CGAAGAGCCC ACCCGAGGCC GCCAGGGCCC TCGCCAGCCC GAAGAGCGAG
    GCGGCCTCCG TCAGCACCAC AAACCCTGCC CAGGGCGAGG ACTTTAAAAT GGACGAAGGG
    AACTTCAAGA CCCCAGATAT TGACCTTGCC AAGGATGTTT TTGCAGCCCT GGGCTCTCCT
    GCTCCCGCCG CTGGGGCCAG TGGACAAGCC TCTGAGCTTG CTCCTTCCAC GGCCGACGGC
    TCTCTTCCGC CTGCACCAGT CAAGACCGAG AAGGGTCCCG TAGAAGCAAA GCCGGAGACA
    GAAACGAAGC CAGCGCCAGC CGAAGTCCAG ACGGTCCCCA ACGATGCCAC ACAAATAAAG
    GAGAATGAGA GCAAAGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45269
    Clone ID Related Accession (Same CDS sequence) XM_010812345.2
    Accession Version XM_010812345.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3369bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCGGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810647.1
    CDS304..3672
    Translation

    Target ORF information:

    RefSeq Version XM_010812345.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812345.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCAAGGGGAA CCCAGTGCAC CTAAGCTCGA AGGGCAGATG
    GGAGAGGATG GAAACTCTAT TAAGGTGAAG CTGATCAAGC AAGACGACGG CGGCTCCCCC
    ATCCGACACT ACCTGGTCAA ATACCGAGCG AAGCTCTCCT CCGAGTGGAA ACCAGAGATC
    AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTCAAGTCCC TGGACTGGAA TGCCGAGTAC
    GAGGTCTATG TGGTGGCCGA GAACCAGCAG GGCAAGTCCA AAGCGGCGCA CTTTGTGTTC
    AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC GGGCCTGAGC
    ACTGGCGCCA TCGTGGGCAT CCTCGTCGTG ACCTTCGTCC TGCTCCTGGT GGCCGTGGAC
    GTCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATTGCCGT CAACCTGTGT
    GGCAAAGCCG GGCCCGGAGC CAAGGGCAAG GACATGGAGG AGGGCAAAGC GGCCTTCTCG
    AAAGATGAGT CCAAGGAGCC TATCGTGGAG GTGCGAACGG AGGAGGAGCG GACCCCGAAT
    CACGACGGAG GGAAACACAC GGAGCCGAAC GAGACCACGC CGCTGACGGA GCCCGAGCTG
    CCTGCTGACA CCACAGCCAC TGTGGAGGAC ATGCTGCCTT CTGTCACCAC CGTCACCGCT
    AACTCTGACA CTATCACCGA AACCTTTGCC ACTGCTCAGA ACAGCCCCAC CAGTGAGACC
    ACCACCCTGA CCTCCAGTAT CGCCCCCCCG GTCGCGGCCA CCCCTGATTC AAACTCTGTG
    CCCGCCGGCC AGGCCACCCC GTCCAAGGGG ACCGGCGTCA CTGCCGCCTC CCCGGCCCCG
    GCCCCAGCCC CCAAGGTCGC CCCCCTCGTT GACCTGAGCG ACCCTCCGGC CTCAGCCCCG
    GCCGCCAACA ACCTGTCCTC TAGCGTCCTG GCCAACCAGG GGGCCGTGCT CAGCCCCAGC
    ACCCCGGCCA GCGCGGGCGA GGCCCCCAAG GCCCCTCCAG CCAGCAAGGC AGTCTCCCCG
    TCGGCAGCCA GTCCCCTGGC AGCAGCCCCG ACAGCCCCTG CCCCGGAAGC CCCCCAGGCC
    AAGCAGGAGG CTCCCGGCAC CAAAGGTCCG GACCCCGAGC CCACCCAGCC CGGCGCCTCG
    AAGAGCCCAC CCGAGGCCGC CAGGGCCCTC GCCAGCCCGA AGAGCGAGGC GGCCTCCGTC
    AGCACCACAA ACCCTGCCCA GGGCGAGGAC TTTAAAATGG ACGAAGGGAA CTTCAAGACC
    CCAGATATTG ACCTTGCCAA GGATGTTTTT GCAGCCCTGG GCTCTCCTGC TCCCGCCGCT
    GGGGCCAGTG GACAAGCCTC TGAGCTTGCT CCTTCCACGG CCGACGGCTC TCTTCCGCCT
    GCACCAGTCA AGACCGAGAA GGGTCCCGTA GAAGCAAAGC CGGAGACAGA AACGAAGCCA
    GCGCCAGCCG AAGTCCAGAC GGTCCCCAAC GATGCCACAC AAATAAAGGA GAATGAGAGC
    AAAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45270
    Clone ID Related Accession (Same CDS sequence) XM_010812346.2
    Accession Version XM_010812346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGATTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTCGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGGCC CCTCCAGCCA GCAAGGCAGT CTCCCCGTCG
    GCAGCCAGTC CCCTGGCAGC AGCCCCGACA GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCAG GGCCCTCGCC AGCCCGAAGA GCGAGGCGGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGTCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC GAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810648.1
    CDS304..3669
    Translation

    Target ORF information:

    RefSeq Version XM_010812346.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGAAG CTCTCCTCCG AGTGGAAACC AGAGATCAGG
    CTCCCGTCTG GCAGTGACCA CGTCATGCTC AAGTCCCTGG ACTGGAATGC CGAGTACGAG
    GTCTATGTGG TGGCCGAGAA CCAGCAGGGC AAGTCCAAAG CGGCGCACTT TGTGTTCAGG
    ACCTCGGCCC AGCCCACAGC CATCCCAGCC AACGGCAGCC CCACCTCGGG CCTGAGCACT
    GGCGCCATCG TGGGCATCCT CGTCGTGACC TTCGTCCTGC TCCTGGTGGC CGTGGACGTC
    ACCTGCTACT TCCTGAACAA GTGTGGCCTG CTCATGTGCA TTGCCGTCAA CCTGTGTGGC
    AAAGCCGGGC CCGGAGCCAA GGGCAAGGAC ATGGAGGAGG GCAAAGCGGC CTTCTCGAAA
    GATGAGTCCA AGGAGCCTAT CGTGGAGGTG CGAACGGAGG AGGAGCGGAC CCCGAATCAC
    GACGGAGGGA AACACACGGA GCCGAACGAG ACCACGCCGC TGACGGAGCC CGAGCTGCCT
    GCTGACACCA CAGCCACTGT GGAGGACATG CTGCCTTCTG TCACCACCGT CACCGCTAAC
    TCTGACACTA TCACCGAAAC CTTTGCCACT GCTCAGAACA GCCCCACCAG TGAGACCACC
    ACCCTGACCT CCAGTATCGC CCCCCCGGTC GCGGCCACCC CTGATTCAAA CTCTGTGCCC
    GCCGGCCAGG CCACCCCGTC CAAGGGGACC GGCGTCACTG CCGCCTCCCC GGCCCCGGCC
    CCAGCCCCCA AGGTCGCCCC CCTCGTTGAC CTGAGCGACC CTCCGGCCTC AGCCCCGGCC
    GCCAACAACC TGTCCTCTAG CGTCCTGGCC AACCAGGGGG CCGTGCTCAG CCCCAGCACC
    CCGGCCAGCG CGGGCGAGGC CCCCAAGGCC CCTCCAGCCA GCAAGGCAGT CTCCCCGTCG
    GCAGCCAGTC CCCTGGCAGC AGCCCCGACA GCCCCTGCCC CGGAAGCCCC CCAGGCCAAG
    CAGGAGGCTC CCGGCACCAA AGGTCCGGAC CCCGAGCCCA CCCAGCCCGG CGCCTCGAAG
    AGCCCACCCG AGGCCGCCAG GGCCCTCGCC AGCCCGAAGA GCGAGGCGGC CTCCGTCAGC
    ACCACAAACC CTGCCCAGGG CGAGGACTTT AAAATGGACG AAGGGAACTT CAAGACCCCA
    GATATTGACC TTGCCAAGGA TGTTTTTGCA GCCCTGGGCT CTCCTGCTCC CGCCGCTGGG
    GCCAGTGGAC AAGCCTCTGA GCTTGCTCCT TCCACGGCCG ACGGCTCTCT TCCGCCTGCA
    CCAGTCAAGA CCGAGAAGGG TCCCGTAGAA GCAAAGCCGG AGACAGAAAC GAAGCCAGCG
    CCAGCCGAAG TCCAGACGGT CCCCAACGAT GCCACACAAA TAAAGGAGAA TGAGAGCAAA
    GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45271
    Clone ID Related Accession (Same CDS sequence) XM_010812347.2
    Accession Version XM_010812347.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACGGAGCC GAACGAGACC ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT
    GACACCACAG CCACTGTGGA GGACATGCTG CCTTCTGTCA CCACCGTCAC CGCTAACTCT
    GACACTATCA CCGAAACCTT TGCCACTGCT CAGAACAGCC CCACCAGTGA GACCACCACC
    CTGACCTCCA GTATCGCCCC CCCGGTCGCG GCCACCCCTG ATTCAAACTC TGTGCCCGCC
    GGCCAGGCCA CCCCGTCCAA GGGGACCGGC GTCACTGCCG CCTCCCCGGC CCCGGCCCCA
    GCCCCCAAGG TCGCCCCCCT CGTTGACCTG AGCGACCCTC CGGCCTCAGC CCCGGCCGCC
    AACAACCTGT CCTCTAGCGT CCTGGCCAAC CAGGGGGCCG TGCTCAGCCC CAGCACCCCG
    GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT CCAGCCAGCA AGGCAGTCTC CCCGTCGGCA
    GCCAGTCCCC TGGCAGCAGC CCCGACAGCC CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG
    GAGGCTCCCG GCACCAAAGG TCCGGACCCC GAGCCCACCC AGCCCGGCGC CTCGAAGAGC
    CCACCCGAGG CCGCCAGGGC CCTCGCCAGC CCGAAGAGCG AGGCGGCCTC CGTCAGCACC
    ACAAACCCTG CCCAGGGCGA GGACTTTAAA ATGGACGAAG GGAACTTCAA GACCCCAGAT
    ATTGACCTTG CCAAGGATGT TTTTGCAGCC CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC
    AGTGGACAAG CCTCTGAGCT TGCTCCTTCC ACGGCCGACG GCTCTCTTCC GCCTGCACCA
    GTCAAGACCG AGAAGGGTCC CGTAGAAGCA AAGCCGGAGA CAGAAACGAA GCCAGCGCCA
    GCCGAAGTCC AGACGGTCCC CAACGATGCC ACACAAATAA AGGAGAATGA GAGCAAAGCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810649.1
    CDS303..3665
    Translation

    Target ORF information:

    RefSeq Version XM_010812347.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812347.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGGCTT CGTGGACTCG ACCAGAGAAG
    CAAGAGACCC TGGACGGGCA CATGGTGGTG CGCAGCCACG CCCGCGTGTC GTCCCTGACG
    CTGAAGAGCA TCCAGTACAC CGACGCCGGG GAGTATGTCT GCACTGCCAG CAACACCATC
    GGCCAGGACT CCCAGTCCAT GTACCTCGAA GTGCAATATG CCCCCAAGCT GCAGGGCCCT
    GTGGCTGTGT ACACTTGGGA GGGGAACCAG GTGAACATCA CCTGCGAGGT GTTTGCCTAC
    CCCAGTGCCA CTATCTCGTG GTTCCGAGAC GGTCAGCTGC TGCCCAGCTC CAACTACAGC
    AACATCAAGA TCTACAACAC CCCGTCCGCC AGCTACCTGG AGGTGACCCC AGACTCTGAA
    AATGATTTTG GAAACTACAA CTGTACCGCA GTGAACCGCA TCGGACAGGA GTCTTTGGAA
    TTCATCCTTG TCCAAGCAGA TACTCCATCC TCACCATCCA TCGACCAGGT GGAGCCATAC
    TCTAGCACAG CACAGGTGCA ATTTGATGAG CCAGAGGCCA CAGGCGGAGT GCCCATCCTC
    AAATACAAAG CTGAGTGGAG AGCAATGGGC GAGGAGGTGT GGCACTCCAA GTGGTATGAT
    GCCAAGGAAG CCAGCATGGA GGGCATTGTC ACCATCGTGG GCCTCAAGCC CGAGACGACG
    TACGCAGTCC GGCTGGCGGC CCTCAACGGC AAGGGCCTGG GCGAGATCAG CGCCGCCTCT
    GAGTTCAAGA CGCAGCCAGT CCGGGAACCC AGTGCACCTA AGCTCGAAGG GCAGATGGGA
    GAGGATGGAA ACTCTATTAA GGTGAAGCTG ATCAAGCAAG ACGACGGCGG CTCCCCCATC
    CGACACTACC TGGTCAAATA CCGAGCGCTC TCCTCCGAGT GGAAACCAGA GATCAGGCTC
    CCGTCTGGCA GTGACCACGT CATGCTCAAG TCCCTGGACT GGAATGCCGA GTACGAGGTC
    TATGTGGTGG CCGAGAACCA GCAGGGCAAG TCCAAAGCGG CGCACTTTGT GTTCAGGACC
    TCGGCCCAGC CCACAGCCAT CCCAGCCAAC GGCAGCCCCA CCTCGGGCCT GAGCACTGGC
    GCCATCGTGG GCATCCTCGT CGTGACCTTC GTCCTGCTCC TGGTGGCCGT GGACGTCACC
    TGCTACTTCC TGAACAAGTG TGGCCTGCTC ATGTGCATTG CCGTCAACCT GTGTGGCAAA
    GCCGGGCCCG GAGCCAAGGG CAAGGACATG GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT
    GAGTCCAAGG AGCCTATCGT GGAGGTGCGA ACGGAGGAGG AGCGGACCCC GAATCACGAC
    GGAGGGAAAC ACACGGAGCC GAACGAGACC ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT
    GACACCACAG CCACTGTGGA GGACATGCTG CCTTCTGTCA CCACCGTCAC CGCTAACTCT
    GACACTATCA CCGAAACCTT TGCCACTGCT CAGAACAGCC CCACCAGTGA GACCACCACC
    CTGACCTCCA GTATCGCCCC CCCGGTCGCG GCCACCCCTG ATTCAAACTC TGTGCCCGCC
    GGCCAGGCCA CCCCGTCCAA GGGGACCGGC GTCACTGCCG CCTCCCCGGC CCCGGCCCCA
    GCCCCCAAGG TCGCCCCCCT CGTTGACCTG AGCGACCCTC CGGCCTCAGC CCCGGCCGCC
    AACAACCTGT CCTCTAGCGT CCTGGCCAAC CAGGGGGCCG TGCTCAGCCC CAGCACCCCG
    GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT CCAGCCAGCA AGGCAGTCTC CCCGTCGGCA
    GCCAGTCCCC TGGCAGCAGC CCCGACAGCC CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG
    GAGGCTCCCG GCACCAAAGG TCCGGACCCC GAGCCCACCC AGCCCGGCGC CTCGAAGAGC
    CCACCCGAGG CCGCCAGGGC CCTCGCCAGC CCGAAGAGCG AGGCGGCCTC CGTCAGCACC
    ACAAACCCTG CCCAGGGCGA GGACTTTAAA ATGGACGAAG GGAACTTCAA GACCCCAGAT
    ATTGACCTTG CCAAGGATGT TTTTGCAGCC CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC
    AGTGGACAAG CCTCTGAGCT TGCTCCTTCC ACGGCCGACG GCTCTCTTCC GCCTGCACCA
    GTCAAGACCG AGAAGGGTCC CGTAGAAGCA AAGCCGGAGA CAGAAACGAA GCCAGCGCCA
    GCCGAAGTCC AGACGGTCCC CAACGATGCC ACACAAATAA AGGAGAATGA GAGCAAAGCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45280
    Clone ID Related Accession (Same CDS sequence) XM_010812356.2
    Accession Version XM_010812356.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3333bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product neural cell adhesion molecule 1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104408.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT GACACCACAG CCACTGTGGA GGACATGCTG
    CCTTCTGTCA CCACCGTCAC CGCTAACTCT GACACTATCA CCGAAACCTT TGCCACTGCT
    CAGAACAGCC CCACCAGTGA GACCACCACC CTGACCTCCA GTATCGCCCC CCCGGTCGCG
    GCCACCCCTG ATTCAAACTC TGTGCCCGCC GGCCAGGCCA CCCCGTCCAA GGGGACCGGC
    GTCACTGCCG CCTCCCCGGC CCCGGCCCCA GCCCCCAAGG TCGCCCCCCT CGTTGACCTG
    AGCGACCCTC CGGCCTCAGC CCCGGCCGCC AACAACCTGT CCTCTAGCGT CCTGGCCAAC
    CAGGGGGCCG TGCTCAGCCC CAGCACCCCG GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT
    CCAGCCAGCA AGGCAGTCTC CCCGTCGGCA GCCAGTCCCC TGGCAGCAGC CCCGACAGCC
    CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG GAGGCTCCCG GCACCAAAGG TCCGGACCCC
    GAGCCCACCC AGCCCGGCGC CTCGAAGAGC CCACCCGAGG CCGCCAGGGC CCTCGCCAGC
    CCGAAGAGCG AGGCGGCCTC CGTCAGCACC ACAAACCCTG CCCAGGGCGA GGACTTTAAA
    ATGGACGAAG GGAACTTCAA GACCCCAGAT ATTGACCTTG CCAAGGATGT TTTTGCAGCC
    CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC AGTGGACAAG CCTCTGAGCT TGCTCCTTCC
    ACGGCCGACG GCTCTCTTCC GCCTGCACCA GTCAAGACCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACGAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810658.1
    CDS303..3635
    Translation

    Target ORF information:

    RefSeq Version XM_010812356.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural cell adhesion molecule 1 (NCAM1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCTGCAAA CTAAGAATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
    CAAGTGGATA TTGTTCCCAG CCAAGGGGAA ATCAGCGTGG GAGAGTCCAA ATTCTTCCTG
    TGCCAAGTGG CCGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCGCC CAACGGAGAG
    AAGCTCACCC CAAACCAGCA GCGGATCTCG GTGGTATGGA ACGATGACTC CTCCTCCACC
    CTCACCATCT ACAACGCCAA CATCGATGAT GCCGGCATCT ACAAGTGTGT GGTCACTGCG
    GAGGATGGCA CCGAGTCAGA GGCCACGGTC AACGTGAAGA TCTTCCAGAA GCTCATGTTC
    AAGAACGCGC CAACCCCACA GGAGTTCAGG GAGGGAGAAG ATGCCGTGAT CGTGTGTGAT
    GTGGTCAGCT CCCTCCCCCC AACCATCATC TGGAAGCACA AAGGCCGAGA TGTCATCCTG
    AAAAAAGATG TCCGATTCAT AGTCCTGACC AACAACTACC TCCAGATCCG GGGCATCAAG
    AAAACAGATG AGGGCACTTA CCGCTGTGAG GGCAGGATCC TGGCCCGCGG GGAGATCAAC
    TTCAAGGACA TTCAGGTCAT CGTGAATGTG CCACCCACCG TCCAGGCCAG ACAGAGCATC
    GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTCG TGTGCAACGC CGAAGGCTTC
    CCAGAGCCCA CCGTGAGCTG GACGAAGGAC GGGGAGCAGA TAGAGAATGA GGAGGACGAG
    AAGTACCTGT TCAGCGACGA CAGCTCCGAG CTGACCATCA GGAAGGTGGA CAAGAACGAC
    GAGGCTGAGT ACGTCTGCAT TGCCGAGAAC AAGGCGGGCG AGCAGGACGC GTCCATCCAC
    CTCAAGGTCT TCGCAAAACC CAAAATCACC TACGTAGAGA ACCAGACTGC CATGGAACTG
    GAGGAACAGG TCACTCTTAC CTGTGAAGCC TCGGGAGACC CCATTCCCTC AATCACCTGG
    AGAACATCCA CCCGAAACAT CAGCAGTGAA GAAAAGACCC TGGACGGGCA CATGGTGGTG
    CGCAGCCACG CCCGCGTGTC GTCCCTGACG CTGAAGAGCA TCCAGTACAC CGACGCCGGG
    GAGTATGTCT GCACTGCCAG CAACACCATC GGCCAGGACT CCCAGTCCAT GTACCTCGAA
    GTGCAATATG CCCCCAAGCT GCAGGGCCCT GTGGCTGTGT ACACTTGGGA GGGGAACCAG
    GTGAACATCA CCTGCGAGGT GTTTGCCTAC CCCAGTGCCA CTATCTCGTG GTTCCGAGAC
    GGTCAGCTGC TGCCCAGCTC CAACTACAGC AACATCAAGA TCTACAACAC CCCGTCCGCC
    AGCTACCTGG AGGTGACCCC AGACTCTGAA AATGATTTTG GAAACTACAA CTGTACCGCA
    GTGAACCGCA TCGGACAGGA GTCTTTGGAA TTCATCCTTG TCCAAGCAGA TACTCCATCC
    TCACCATCCA TCGACCAGGT GGAGCCATAC TCTAGCACAG CACAGGTGCA ATTTGATGAG
    CCAGAGGCCA CAGGCGGAGT GCCCATCCTC AAATACAAAG CTGAGTGGAG AGCAATGGGC
    GAGGAGGTGT GGCACTCCAA GTGGTATGAT GCCAAGGAAG CCAGCATGGA GGGCATTGTC
    ACCATCGTGG GCCTCAAGCC CGAGACGACG TACGCAGTCC GGCTGGCGGC CCTCAACGGC
    AAGGGCCTGG GCGAGATCAG CGCCGCCTCT GAGTTCAAGA CGCAGCCAGT CCGGGAACCC
    AGTGCACCTA AGCTCGAAGG GCAGATGGGA GAGGATGGAA ACTCTATTAA GGTGAAGCTG
    ATCAAGCAAG ACGACGGCGG CTCCCCCATC CGACACTACC TGGTCAAATA CCGAGCGCTC
    TCCTCCGAGT GGAAACCAGA GATCAGGCTC CCGTCTGGCA GTGACCACGT CATGCTCAAG
    TCCCTGGACT GGAATGCCGA GTACGAGGTC TATGTGGTGG CCGAGAACCA GCAGGGCAAG
    TCCAAAGCGG CGCACTTTGT GTTCAGGACC TCGGCCCAGC CCACAGCCAT CCCAGCCAAC
    GGCAGCCCCA CCTCGGGCCT GAGCACTGGC GCCATCGTGG GCATCCTCGT CGTGACCTTC
    GTCCTGCTCC TGGTGGCCGT GGACGTCACC TGCTACTTCC TGAACAAGTG TGGCCTGCTC
    ATGTGCATTG CCGTCAACCT GTGTGGCAAA GCCGGGCCCG GAGCCAAGGG CAAGGACATG
    GAGGAGGGCA AAGCGGCCTT CTCGAAAGAT GAGTCCAAGG AGCCTATCGT GGAGGTGCGA
    ACGGAGGAGG AGCGGACCCC GAATCACGAC GGAGGGAAAC ACACGGAGCC GAACGAGACC
    ACGCCGCTGA CGGAGCCCGA GCTGCCTGCT GACACCACAG CCACTGTGGA GGACATGCTG
    CCTTCTGTCA CCACCGTCAC CGCTAACTCT GACACTATCA CCGAAACCTT TGCCACTGCT
    CAGAACAGCC CCACCAGTGA GACCACCACC CTGACCTCCA GTATCGCCCC CCCGGTCGCG
    GCCACCCCTG ATTCAAACTC TGTGCCCGCC GGCCAGGCCA CCCCGTCCAA GGGGACCGGC
    GTCACTGCCG CCTCCCCGGC CCCGGCCCCA GCCCCCAAGG TCGCCCCCCT CGTTGACCTG
    AGCGACCCTC CGGCCTCAGC CCCGGCCGCC AACAACCTGT CCTCTAGCGT CCTGGCCAAC
    CAGGGGGCCG TGCTCAGCCC CAGCACCCCG GCCAGCGCGG GCGAGGCCCC CAAGGCCCCT
    CCAGCCAGCA AGGCAGTCTC CCCGTCGGCA GCCAGTCCCC TGGCAGCAGC CCCGACAGCC
    CCTGCCCCGG AAGCCCCCCA GGCCAAGCAG GAGGCTCCCG GCACCAAAGG TCCGGACCCC
    GAGCCCACCC AGCCCGGCGC CTCGAAGAGC CCACCCGAGG CCGCCAGGGC CCTCGCCAGC
    CCGAAGAGCG AGGCGGCCTC CGTCAGCACC ACAAACCCTG CCCAGGGCGA GGACTTTAAA
    ATGGACGAAG GGAACTTCAA GACCCCAGAT ATTGACCTTG CCAAGGATGT TTTTGCAGCC
    CTGGGCTCTC CTGCTCCCGC CGCTGGGGCC AGTGGACAAG CCTCTGAGCT TGCTCCTTCC
    ACGGCCGACG GCTCTCTTCC GCCTGCACCA GTCAAGACCG AGAAGGGTCC CGTAGAAGCA
    AAGCCGGAGA CAGAAACGAA GCCAGCGCCA GCCGAAGTCC AGACGGTCCC CAACGATGCC
    ACACAAATAA AGGAGAATGA GAGCAAAGCG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.