GRK5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK5
Entrez Gene ID	281801
Full Name	G protein-coupled receptor kinase 5
Synonyms	GPRK5
General protein information	Preferred Names G protein-coupled receptor kinase 5 Names G protein-coupled receptor kinase 5 g protein-coupled receptor kinase GRK5
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010820077.2	XP_010818379.1	G protein-coupled receptor kinase 5 isoform X1
NM_174331.2	NP_776756.1	G protein-coupled receptor kinase 5
XM_010820077.3	XP_010818379.1	G protein-coupled receptor kinase 5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRK5	XP_003312835.1
Bos taurus (cattle)	GRK5	NP_776756.1
Xenopus tropicalis (tropical clawed frog)	LOC100497344	XP_002940394.1
	LOC100427251	XP_002805891.1
Mus musculus (house mouse)	Grk5	NP_061357.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004117	XP_312995.5
Caenorhabditis elegans (roundworm)	grk-1	NP_509676.1
Homo sapiens (human)	GRK5	NP_005299.1
Canis lupus familiaris (dog)	GRK5	XP_544045.3
Rattus norvegicus (Norway rat)	Grk5	NP_110456.1
Gallus gallus (chicken)	GRK5	XP_421789.2
Drosophila melanogaster (fruit fly)	Gprk2	NP_476867.1

Gene Ontology
Bibliography

Related articles in PubMed

GRK5-mediated exacerbation of pathological cardiac hypertrophy involves facilitation of nuclear NFAT activity.
Hullmann JE, Grisanti LA, Makarewich CA, Gao E, Gold JI, Chuprun JK, Tilley DG, Houser SR, Koch WJ
Circulation research115(12)976-85(2014 Dec)

Targeted ubiquitination and degradation of G-protein-coupled receptor kinase 5 by the DDB1-CUL4 ubiquitin ligase complex.
Wu Z, Chen Y, Yang T, Gao Q, Yuan M, Ma L
PloS one7(8)e43997(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha.
Sorriento D, Ciccarelli M, Santulli G, Campanile A, Altobelli GG, Cimini V, Galasso G, Astone D, Piscione F, Pastore L, Trimarco B, Iaccarino G
Proceedings of the National Academy of Sciences of the United States of America105(46)17818-23(2008 Nov)

Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases.
Premont RT, Koch WJ, Inglese J, Lefkowitz RJ
The Journal of biological chemistry269(9)6832-41(1994 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GRK5, acting in a kinase independent manner, is a facilitator of NFAT activity and part of a DNA-binding complex responsible for pathological hypertrophic gene transcription.
Title: GRK5-mediated exacerbation of pathological cardiac hypertrophy involves facilitation of nuclear NFAT activity.

potential GRK5 interacting proteins and the association of GRK5 with DDB1 in cell and the regulation of GRK5 level by DDB1-CUL4 ubiquitin ligase complex-dependent proteolysis pathway
Title: Targeted ubiquitination and degradation of G-protein-coupled receptor kinase 5 by the DDB1-CUL4 ubiquitin ligase complex.

GRK5 overexpression causes nuclear accumulation of IkappaB alpha, leading to the inhibition of NFkappaB transcriptional activity.
Title: The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha.

The following GRK5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43346	NM_174331.2 Latest version!	Bos taurus G protein-coupled receptor kinase 5 (GRK5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45239	XM_010820077.3 Latest version!	Bos taurus G protein-coupled receptor kinase 5 (GRK5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa45239	XM_010820077.2	Bos taurus G protein-coupled receptor kinase 5 (GRK5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK5 ( NM_174331.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776756.1 Bos taurus G protein-coupled receptor kinase 5 (GRK5), mRNA.

Price & Availability↑

CloneID	OBa43346
Clone ID Related Accession (Same CDS sequence)	NM_174331.2
Accession Version	NM_174331.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U01206.1. On Jun 3, 2003 this sequence version replaced NM_174331.1. ##Evidence-Data-START## Transcript exon combination :: U01206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCAGGGA AGGAGGCGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTCCC TCACATAAAC 
CAGTGTGAAG ACCTCCGAAG AACCATAGAC CGAGATTACT GCAGTTTATG TGACAAACAG 
CCAGTCGGGA GGCTGCTTTT CCGGCAGTTC TGCGAAACCA GACCTGGGCT GGAGTCCTAC 
ATTCAGTTCC TGGACTCGGT GGCAGAATAT GAAGTTACTC CAGATGAAAA ACTGGGAGAA 
AAAGGGAAGG AAATTATGAC CAAGTACCTC ACCCCAAAGT CCCCAGTTTT CATCACCCAA 
GTTGGACGGG ACCTGGTCTC CCAGACGGAG GAGAAGCTCC TTCAGAAACC CTGCAAAGAA 
CTCTTCTCGG CCTGCGTGCA GTCTGTCCAC GACTACCTGA GGGGAGAACC CTTCCACGAG 
TACCTGGACA GCATGTATTT TGATCGCTTT CTCCAGTGGA AGTGGTTGGA AAGGCAACCG 
GTAACCAAAA ACACTTTCAG GCAGTACCGA GTACTAGGAA AAGGGGGCTT TGGGGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACGGGTAAA ATGTACGCCT GCAAACGCTT GGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG GGAGTCCATG GCCCTGAATG AGAAGCAGAT TCTGGAGAAG 
GTCAACAGTC GGTTTGTGGT CAACCTGGCC TACGCCTACG AGACCAAGGA TGCCCTGTGT 
TTGGTCCTGA CCATCATGAA CGGCGGGGAC CTGAAGTTCC ACATTTACAA CATGGGGAAC 
CCCGGCTTCG AGGAGGAGCG CGCCCTGTTC TACGCGGCAG AGATCCTGTG CGGCCTGGAG 
GACCTCCACC ATGAGAACAT TGTCTACAGA GATTTGAAAC CTGAAAATAT CCTGTTAGAC 
GATTACGGCC ACATTAGAAT TTCGGACCTG GGCCTGGCCG TGAAGATCCC CGAGGGAGAC 
CTGATTCGCG GCCGAGTGGG CACCGTTGGC TACATGGCTC CAGAGGTCCT GAACAACCAG 
AGGTATGGCC TGAGCCCCGA CTACTGGGGT CTGGGCTGCC TCATCTACGA GATGATCGAG 
GGCCAGTCGC CCTTCCGCGG CCGCAAGGAG AAGGTGAAGC GGGAGGAGGT GGACCGCCGG 
GTGTTGGAGA CAGAGGAGGT GTACTCGCAC AAGTTCTCCG AGGAGGCCAA GTCCATCTGC 
AAAATGCTGC TCACCAAAGA TGCAAAGCAG AGGCTGGGCT GCCAGGAGGA AGGGGCCGCG 
GAGGTCAAGA GACACCCCTT CTTCAGGAAC ATGAATTTCA AGCGCTTAGA GGCCGGGATG 
TTGGATCCCC CCTTTGTTCC AGATCCCCGG GCCGTGTACT GCAAGGACGT CTTGGACATC 
GAGCAGTTCT CCACCGTGAA GGGCGTCAAC CTGGACCACA CGGACGACGA CTTCTACTCC 
AAGTTCTCCA CGGGCTCTGT GCCCATCCCG TGGCAAAGCG AGATGATAGA AACAGAGTGC 
TTTAAGGAGC TGAACGTGTT CGGACCCCAC GGTACCCTCT CACCGGACCT GAACCGAAGC 
CACCCTCCGG AACCGCCAAA GAAAGGGCTG CTCCAGAGGC TCTTCAAGCG TCAGCATCAA 
AACAATTCCA AGAGTTCACC CAATTCCAAG ACCAGTTTTA ACCACCACAT CAATTCAAAC 
CACGTGAGTT CAAACTCCAC CGGAAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776756.1
CDS	175..1947
Translation	MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHINQCEDLRRTIDRDYCSLCDKQPVGRLLFRQFCETRPGLESYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFITQVGRDLVSQTEEKLLQKPCKELFSACVQSVHDYLRGEPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSRFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHHENIVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQSEMIETECFKELNVFGPHGTLSPDLNRSHPPEPPKKGLLQRLFKRQHQNNSKSSPNSKTSFNHHINSNHVSSNSTGSS

Target ORF information:

RefSeq Version	NM_174331.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 5 (GRK5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174331.2

ORF Insert Sequence:

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCAGGGA AGGAGGCGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTCCC TCACATAAAC 
CAGTGTGAAG ACCTCCGAAG AACCATAGAC CGAGATTACT GCAGTTTATG TGACAAACAG 
CCAGTCGGGA GGCTGCTTTT CCGGCAGTTC TGCGAAACCA GACCTGGGCT GGAGTCCTAC 
ATTCAGTTCC TGGACTCGGT GGCAGAATAT GAAGTTACTC CAGATGAAAA ACTGGGAGAA 
AAAGGGAAGG AAATTATGAC CAAGTACCTC ACCCCAAAGT CCCCAGTTTT CATCACCCAA 
GTTGGACGGG ACCTGGTCTC CCAGACGGAG GAGAAGCTCC TTCAGAAACC CTGCAAAGAA 
CTCTTCTCGG CCTGCGTGCA GTCTGTCCAC GACTACCTGA GGGGAGAACC CTTCCACGAG 
TACCTGGACA GCATGTATTT TGATCGCTTT CTCCAGTGGA AGTGGTTGGA AAGGCAACCG 
GTAACCAAAA ACACTTTCAG GCAGTACCGA GTACTAGGAA AAGGGGGCTT TGGGGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACGGGTAAA ATGTACGCCT GCAAACGCTT GGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG GGAGTCCATG GCCCTGAATG AGAAGCAGAT TCTGGAGAAG 
GTCAACAGTC GGTTTGTGGT CAACCTGGCC TACGCCTACG AGACCAAGGA TGCCCTGTGT 
TTGGTCCTGA CCATCATGAA CGGCGGGGAC CTGAAGTTCC ACATTTACAA CATGGGGAAC 
CCCGGCTTCG AGGAGGAGCG CGCCCTGTTC TACGCGGCAG AGATCCTGTG CGGCCTGGAG 
GACCTCCACC ATGAGAACAT TGTCTACAGA GATTTGAAAC CTGAAAATAT CCTGTTAGAC 
GATTACGGCC ACATTAGAAT TTCGGACCTG GGCCTGGCCG TGAAGATCCC CGAGGGAGAC 
CTGATTCGCG GCCGAGTGGG CACCGTTGGC TACATGGCTC CAGAGGTCCT GAACAACCAG 
AGGTATGGCC TGAGCCCCGA CTACTGGGGT CTGGGCTGCC TCATCTACGA GATGATCGAG 
GGCCAGTCGC CCTTCCGCGG CCGCAAGGAG AAGGTGAAGC GGGAGGAGGT GGACCGCCGG 
GTGTTGGAGA CAGAGGAGGT GTACTCGCAC AAGTTCTCCG AGGAGGCCAA GTCCATCTGC 
AAAATGCTGC TCACCAAAGA TGCAAAGCAG AGGCTGGGCT GCCAGGAGGA AGGGGCCGCG 
GAGGTCAAGA GACACCCCTT CTTCAGGAAC ATGAATTTCA AGCGCTTAGA GGCCGGGATG 
TTGGATCCCC CCTTTGTTCC AGATCCCCGG GCCGTGTACT GCAAGGACGT CTTGGACATC 
GAGCAGTTCT CCACCGTGAA GGGCGTCAAC CTGGACCACA CGGACGACGA CTTCTACTCC 
AAGTTCTCCA CGGGCTCTGT GCCCATCCCG TGGCAAAGCG AGATGATAGA AACAGAGTGC 
TTTAAGGAGC TGAACGTGTT CGGACCCCAC GGTACCCTCT CACCGGACCT GAACCGAAGC 
CACCCTCCGG AACCGCCAAA GAAAGGGCTG CTCCAGAGGC TCTTCAAGCG TCAGCATCAA 
AACAATTCCA AGAGTTCACC CAATTCCAAG ACCAGTTTTA ACCACCACAT CAATTCAAAC 
CACGTGAGTT CAAACTCCAC CGGAAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK5 ( XM_010820077.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818379.1 Bos taurus G protein-coupled receptor kinase 5 (GRK5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45239
Clone ID Related Accession (Same CDS sequence)	XM_010820077.2 , XM_010820077.3
Accession Version	XM_010820077.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104584.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCAGGGA AGGAGGCGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTCCC TCACATAAAC 
CAGTGTGAAG ACCTCCGAAG AACCATAGAC CGAGATTACT GCAGTTTATG TGACAAACAG 
CCAGTCGGGA GGCTGCTTTT CCGGCAGTTC TGCGAAACCA GACCTGGGCT GGAGTCCTAC 
ATTCAGTTCC TGGACTCGGT GGCAGAATAT GAAGTTACTC CAGATGAAAA ACTGGGAGAA 
AAAGGGAAGG AAATTATGAC CAAGTACCTC ACCCCAAAGT CCCCAGTTTT CATCACCCAA 
GTTGGACGGG ACCTGGTCTC CCAGACGGAG GAGAAGCTCC TTCAGAAACC CTGCAAAGAA 
CTCTTCTCGG CCTGCGTGCA GTCTGTCCAC GACTACCTGA GGGGAGAACC CTTCCACGAG 
TACCTGGACA GCATGTATTT TGATCGCTTT CTCCAGTGGA AGTGGTTGGA AAGGCAACCG 
GTAACCAAAA ACACTTTCAG GCAGTACCGA GTACTAGGAA AAGGGGGCTT TGGGGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACGGGTAAA ATGTACGCCT GCAAACGCTT GGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG GGAGTCCATG GCCCTGAATG AGAAGCAGAT TCTGGAGAAG 
GTCAACAGTC GGTTTGTGGT CAACCTGGCC TACGCCTACG AGACCAAGGA TGCCCTGTGT 
TTGGTCCTGA CCATCATGAA CGGCGGGGAC CTGAAGTTCC ACATTTACAA CATGGGGAAC 
CCCGGCTTCG AGGAGGAGCG CGCCCTGTTC TACGCGGCAG AGATCCTGTG CGGCCTGGAG 
GACCTCCACC ATGAGAACAT TGTCTACAGA GATTTGAAAC CTGAAAATAT CCTGTTAGAC 
GATTACGCTC CAGAGGTCCT GAACAACCAG AGGTATGGCC TGAGCCCCGA CTACTGGGGT 
CTGGGCTGCC TCATCTACGA GATGATCGAG GGCCAGTCGC CCTTCCGCGG CCGCAAGGAG 
AAGGTGAAGC GGGAGGAGGT GGACCGCCGG GTGTTGGAGA CAGAGGAGGT GTACTCGCAC 
AAGTTCTCCG AGGAGGCCAA GTCCATCTGC AAAATGCTGC TCACCAAAGA TGCAAAGCAG 
AGGCTGGGCT GCCAGGAGGA AGGGGCCGCG GAGGTCAAGA GACACCCCTT CTTCAGGAAC 
ATGAATTTCA AGCGCTTAGA GGCCGGGATG TTGGATCCCC CCTTTGTTCC AGATCCCCGG 
GCCGTGTACT GCAAGGACGT CTTGGACATC GAGCAGTTCT CCACCGTGAA GGGCGTCAAC 
CTGGACCACA CGGACGACGA CTTCTACTCC AAGTTCTCCA CGGGCTCTGT GCCCATCCCG 
TGGCAAAGCG AGATGATAGA AACAGAGTGC TTTAAGGAGC TGAACGTGTT CGGACCCCAC 
GGTACCCTCT CACCGGACCT GAACCGAAGC CACCCTCCGG AACCGCCAAA GAAAGGGCTG 
CTCCAGAGGC TCTTCAAGCG TCAGCATCAA AACAATTCCA AGAGTTCACC CAATTCCAAG 
ACCAGTTTTA ACCACCACAT CAATTCAAAC CACGTGAGTT CAAACTCCAC CGGAAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818379.1
CDS	231..1913
Translation	MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHINQCEDLRRTIDRDYCSLCDKQPVGRLLFRQFCETRPGLESYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFITQVGRDLVSQTEEKLLQKPCKELFSACVQSVHDYLRGEPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSRFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHHENIVYRDLKPENILLDDYAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQSEMIETECFKELNVFGPHGTLSPDLNRSHPPEPPKKGLLQRLFKRQHQNNSKSSPNSKTSFNHHINSNHVSSNSTGSS

Target ORF information:

RefSeq Version	XM_010820077.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 5 (GRK5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820077.2

ORF Insert Sequence:

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCAGGGA AGGAGGCGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTCCC TCACATAAAC 
CAGTGTGAAG ACCTCCGAAG AACCATAGAC CGAGATTACT GCAGTTTATG TGACAAACAG 
CCAGTCGGGA GGCTGCTTTT CCGGCAGTTC TGCGAAACCA GACCTGGGCT GGAGTCCTAC 
ATTCAGTTCC TGGACTCGGT GGCAGAATAT GAAGTTACTC CAGATGAAAA ACTGGGAGAA 
AAAGGGAAGG AAATTATGAC CAAGTACCTC ACCCCAAAGT CCCCAGTTTT CATCACCCAA 
GTTGGACGGG ACCTGGTCTC CCAGACGGAG GAGAAGCTCC TTCAGAAACC CTGCAAAGAA 
CTCTTCTCGG CCTGCGTGCA GTCTGTCCAC GACTACCTGA GGGGAGAACC CTTCCACGAG 
TACCTGGACA GCATGTATTT TGATCGCTTT CTCCAGTGGA AGTGGTTGGA AAGGCAACCG 
GTAACCAAAA ACACTTTCAG GCAGTACCGA GTACTAGGAA AAGGGGGCTT TGGGGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACGGGTAAA ATGTACGCCT GCAAACGCTT GGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG GGAGTCCATG GCCCTGAATG AGAAGCAGAT TCTGGAGAAG 
GTCAACAGTC GGTTTGTGGT CAACCTGGCC TACGCCTACG AGACCAAGGA TGCCCTGTGT 
TTGGTCCTGA CCATCATGAA CGGCGGGGAC CTGAAGTTCC ACATTTACAA CATGGGGAAC 
CCCGGCTTCG AGGAGGAGCG CGCCCTGTTC TACGCGGCAG AGATCCTGTG CGGCCTGGAG 
GACCTCCACC ATGAGAACAT TGTCTACAGA GATTTGAAAC CTGAAAATAT CCTGTTAGAC 
GATTACGCTC CAGAGGTCCT GAACAACCAG AGGTATGGCC TGAGCCCCGA CTACTGGGGT 
CTGGGCTGCC TCATCTACGA GATGATCGAG GGCCAGTCGC CCTTCCGCGG CCGCAAGGAG 
AAGGTGAAGC GGGAGGAGGT GGACCGCCGG GTGTTGGAGA CAGAGGAGGT GTACTCGCAC 
AAGTTCTCCG AGGAGGCCAA GTCCATCTGC AAAATGCTGC TCACCAAAGA TGCAAAGCAG 
AGGCTGGGCT GCCAGGAGGA AGGGGCCGCG GAGGTCAAGA GACACCCCTT CTTCAGGAAC 
ATGAATTTCA AGCGCTTAGA GGCCGGGATG TTGGATCCCC CCTTTGTTCC AGATCCCCGG 
GCCGTGTACT GCAAGGACGT CTTGGACATC GAGCAGTTCT CCACCGTGAA GGGCGTCAAC 
CTGGACCACA CGGACGACGA CTTCTACTCC AAGTTCTCCA CGGGCTCTGT GCCCATCCCG 
TGGCAAAGCG AGATGATAGA AACAGAGTGC TTTAAGGAGC TGAACGTGTT CGGACCCCAC 
GGTACCCTCT CACCGGACCT GAACCGAAGC CACCCTCCGG AACCGCCAAA GAAAGGGCTG 
CTCCAGAGGC TCTTCAAGCG TCAGCATCAA AACAATTCCA AGAGTTCACC CAATTCCAAG 
ACCAGTTTTA ACCACCACAT CAATTCAAAC CACGTGAGTT CAAACTCCAC CGGAAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK5 ( XM_010820077.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818379.1 Bos taurus G protein-coupled receptor kinase 5 (GRK5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45239
Clone ID Related Accession (Same CDS sequence)	XM_010820077.2 , XM_010820077.3
Accession Version	XM_010820077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	G protein-coupled receptor kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCAGGGA AGGAGGCGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTCCC TCACATAAAC 
CAGTGTGAAG ACCTCCGAAG AACCATAGAC CGAGATTACT GCAGTTTATG TGACAAACAG 
CCAGTCGGGA GGCTGCTTTT CCGGCAGTTC TGCGAAACCA GACCTGGGCT GGAGTCCTAC 
ATTCAGTTCC TGGACTCGGT GGCAGAATAT GAAGTTACTC CAGATGAAAA ACTGGGAGAA 
AAAGGGAAGG AAATTATGAC CAAGTACCTC ACCCCAAAGT CCCCAGTTTT CATCACCCAA 
GTTGGACGGG ACCTGGTCTC CCAGACGGAG GAGAAGCTCC TTCAGAAACC CTGCAAAGAA 
CTCTTCTCGG CCTGCGTGCA GTCTGTCCAC GACTACCTGA GGGGAGAACC CTTCCACGAG 
TACCTGGACA GCATGTATTT TGATCGCTTT CTCCAGTGGA AGTGGTTGGA AAGGCAACCG 
GTAACCAAAA ACACTTTCAG GCAGTACCGA GTACTAGGAA AAGGGGGCTT TGGGGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACGGGTAAA ATGTACGCCT GCAAACGCTT GGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG GGAGTCCATG GCCCTGAATG AGAAGCAGAT TCTGGAGAAG 
GTCAACAGTC GGTTTGTGGT CAACCTGGCC TACGCCTACG AGACCAAGGA TGCCCTGTGT 
TTGGTCCTGA CCATCATGAA CGGCGGGGAC CTGAAGTTCC ACATTTACAA CATGGGGAAC 
CCCGGCTTCG AGGAGGAGCG CGCCCTGTTC TACGCGGCAG AGATCCTGTG CGGCCTGGAG 
GACCTCCACC ATGAGAACAT TGTCTACAGA GATTTGAAAC CTGAAAATAT CCTGTTAGAC 
GATTACGCTC CAGAGGTCCT GAACAACCAG AGGTATGGCC TGAGCCCCGA CTACTGGGGT 
CTGGGCTGCC TCATCTACGA GATGATCGAG GGCCAGTCGC CCTTCCGCGG CCGCAAGGAG 
AAGGTGAAGC GGGAGGAGGT GGACCGCCGG GTGTTGGAGA CAGAGGAGGT GTACTCGCAC 
AAGTTCTCCG AGGAGGCCAA GTCCATCTGC AAAATGCTGC TCACCAAAGA TGCAAAGCAG 
AGGCTGGGCT GCCAGGAGGA AGGGGCCGCG GAGGTCAAGA GACACCCCTT CTTCAGGAAC 
ATGAATTTCA AGCGCTTAGA GGCCGGGATG TTGGATCCCC CCTTTGTTCC AGATCCCCGG 
GCCGTGTACT GCAAGGACGT CTTGGACATC GAGCAGTTCT CCACCGTGAA GGGCGTCAAC 
CTGGACCACA CGGACGACGA CTTCTACTCC AAGTTCTCCA CGGGCTCTGT GCCCATCCCG 
TGGCAAAGCG AGATGATAGA AACAGAGTGC TTTAAGGAGC TGAACGTGTT CGGACCCCAC 
GGTACCCTCT CACCGGACCT GAACCGAAGC CACCCTCCGG AACCGCCAAA GAAAGGGCTG 
CTCCAGAGGC TCTTCAAGCG TCAGCATCAA AACAATTCCA AGAGTTCACC CAATTCCAAG 
ACCAGTTTTA ACCACCACAT CAATTCAAAC CACGTGAGTT CAAACTCCAC CGGAAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818379.1
CDS	228..1910
Translation	MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHINQCEDLRRTIDRDYCSLCDKQPVGRLLFRQFCETRPGLESYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFITQVGRDLVSQTEEKLLQKPCKELFSACVQSVHDYLRGEPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSRFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHHENIVYRDLKPENILLDDYAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQSEMIETECFKELNVFGPHGTLSPDLNRSHPPEPPKKGLLQRLFKRQHQNNSKSSPNSKTSFNHHINSNHVSSNSTGSS

Target ORF information:

RefSeq Version	XM_010820077.3
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 5 (GRK5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820077.3

ORF Insert Sequence:

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCAGGGA AGGAGGCGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTCCC TCACATAAAC 
CAGTGTGAAG ACCTCCGAAG AACCATAGAC CGAGATTACT GCAGTTTATG TGACAAACAG 
CCAGTCGGGA GGCTGCTTTT CCGGCAGTTC TGCGAAACCA GACCTGGGCT GGAGTCCTAC 
ATTCAGTTCC TGGACTCGGT GGCAGAATAT GAAGTTACTC CAGATGAAAA ACTGGGAGAA 
AAAGGGAAGG AAATTATGAC CAAGTACCTC ACCCCAAAGT CCCCAGTTTT CATCACCCAA 
GTTGGACGGG ACCTGGTCTC CCAGACGGAG GAGAAGCTCC TTCAGAAACC CTGCAAAGAA 
CTCTTCTCGG CCTGCGTGCA GTCTGTCCAC GACTACCTGA GGGGAGAACC CTTCCACGAG 
TACCTGGACA GCATGTATTT TGATCGCTTT CTCCAGTGGA AGTGGTTGGA AAGGCAACCG 
GTAACCAAAA ACACTTTCAG GCAGTACCGA GTACTAGGAA AAGGGGGCTT TGGGGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACGGGTAAA ATGTACGCCT GCAAACGCTT GGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG GGAGTCCATG GCCCTGAATG AGAAGCAGAT TCTGGAGAAG 
GTCAACAGTC GGTTTGTGGT CAACCTGGCC TACGCCTACG AGACCAAGGA TGCCCTGTGT 
TTGGTCCTGA CCATCATGAA CGGCGGGGAC CTGAAGTTCC ACATTTACAA CATGGGGAAC 
CCCGGCTTCG AGGAGGAGCG CGCCCTGTTC TACGCGGCAG AGATCCTGTG CGGCCTGGAG 
GACCTCCACC ATGAGAACAT TGTCTACAGA GATTTGAAAC CTGAAAATAT CCTGTTAGAC 
GATTACGCTC CAGAGGTCCT GAACAACCAG AGGTATGGCC TGAGCCCCGA CTACTGGGGT 
CTGGGCTGCC TCATCTACGA GATGATCGAG GGCCAGTCGC CCTTCCGCGG CCGCAAGGAG 
AAGGTGAAGC GGGAGGAGGT GGACCGCCGG GTGTTGGAGA CAGAGGAGGT GTACTCGCAC 
AAGTTCTCCG AGGAGGCCAA GTCCATCTGC AAAATGCTGC TCACCAAAGA TGCAAAGCAG 
AGGCTGGGCT GCCAGGAGGA AGGGGCCGCG GAGGTCAAGA GACACCCCTT CTTCAGGAAC 
ATGAATTTCA AGCGCTTAGA GGCCGGGATG TTGGATCCCC CCTTTGTTCC AGATCCCCGG 
GCCGTGTACT GCAAGGACGT CTTGGACATC GAGCAGTTCT CCACCGTGAA GGGCGTCAAC 
CTGGACCACA CGGACGACGA CTTCTACTCC AAGTTCTCCA CGGGCTCTGT GCCCATCCCG 
TGGCAAAGCG AGATGATAGA AACAGAGTGC TTTAAGGAGC TGAACGTGTT CGGACCCCAC 
GGTACCCTCT CACCGGACCT GAACCGAAGC CACCCTCCGG AACCGCCAAA GAAAGGGCTG 
CTCCAGAGGC TCTTCAAGCG TCAGCATCAA AACAATTCCA AGAGTTCACC CAATTCCAAG 
ACCAGTTTTA ACCACCACAT CAATTCAAAC CACGTGAGTT CAAACTCCAC CGGAAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GRK5-mediated exacerbation of pathological cardiac hypertrophy involves facilitation of nuclear NFAT activity.
Circulation research115(12)976-85(2014 Dec)
Hullmann JE,Grisanti LA,Makarewich CA,Gao E,Gold JI,Chuprun JK,Tilley DG,Houser SR,Koch WJ

Targeted ubiquitination and degradation of G-protein-coupled receptor kinase 5 by the DDB1-CUL4 ubiquitin ligase complex.
PloS one7(8)e43997(2012)
Wu Z,Chen Y,Yang T,Gao Q,Yuan M,Ma L

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha.
Proceedings of the National Academy of Sciences of the United States of America105(46)17818-23(2008 Nov)
Sorriento D,Ciccarelli M,Santulli G,Campanile A,Altobelli GG,Cimini V,Galasso G,Astone D,Piscione F,Pastore L,Trimarco B,Iaccarino G

Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases.
The Journal of biological chemistry269(9)6832-41(1994 Mar)
Premont RT,Koch WJ,Inglese J,Lefkowitz RJ

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GRK5 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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