GNAI1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNAI1
Entrez Gene ID	281790
Full Name	G protein subunit alpha i1
General protein information	Preferred Names G protein subunit alpha i1 Names guanine nucleotide-binding protein G(i) subunit alpha-1 adenylate cyclase-inhibiting G alpha protein guanine nucleotide-binding protein G(i), alpha-1 subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005205328.3	XP_005205385.1	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X1
XM_015468831.1	XP_015324317.1	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X2
NM_174324.2	NP_776749.1	guanine nucleotide-binding protein G(i) subunit alpha-1
XM_015468832.1	XP_015324318.1	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X3
XM_005205328.4	XP_005205385.1	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X1
XM_015468832.2	XP_015324318.1	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAI1	XP_001159548.1
Danio rerio (zebrafish)	gnai1	NP_957265.1
Rattus norvegicus (Norway rat)	Gnai1	NP_037277.1
Mus musculus (house mouse)	Gnai1	NP_034435.1
Gallus gallus (chicken)	GNAI1	NP_990734.1
Drosophila melanogaster (fruit fly)	G-ialpha65A	NP_477502.1
Xenopus tropicalis (tropical clawed frog)	gnai1	NP_001090865.1
Homo sapiens (human)	GNAI1	NP_002060.4
Bos taurus (cattle)	GNAI1	NP_776749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005904	XP_315935.4
Caenorhabditis elegans (roundworm)	gpa-16	NP_490790.1

Gene Ontology
Bibliography

Related articles in PubMed

NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes.
Smrcka AV, Kichik N, Tarrag? T, Burroughs M, Park MS, Itoga NK, Stern HA, Willardson BM, Giralt E
Proceedings of the National Academy of Sciences of the United States of America107(2)639-44(2010 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Synteny mapping of five human chromosome 7 genes on bovine chromosomes 4 and 21.
Antoniou E, Womack JE, Grosz MD
Cytogenetics and cell genetics84(1-2)121-4(1999)

Molecular cloning and characterization of cDNA encoding the GTP-binding protein alpha i and identification of a related protein, alpha h.
Michel T, Winslow JW, Smith JA, Seidman JG, Neer EJ
Proceedings of the National Academy of Sciences of the United States of America83(20)7663-7(1986 Oct)

Primary structure of the alpha-subunit of bovine adenylate cyclase-inhibiting G-protein deduced from the cDNA sequence.
Nukada T, Tanabe T, Takahashi H, Noda M, Haga K, Haga T, Ichiyama A, Kanagawa K, Hiranaga M, Matsuo
FEBS letters197(1-2)305-10(1986 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNAI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41324	NM_174324.2 Latest version!	Bos taurus G protein subunit alpha i1 (GNAI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa18489	XM_005205328.4 Latest version!	Bos taurus G protein subunit alpha i1 (GNAI1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa41324	XM_015468832.2 Latest version!	Bos taurus G protein subunit alpha i1 (GNAI1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa90593	XM_015468831.1 Latest version!	Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OBa18489	XM_005205328.3	Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa41324	XM_015468832.1	Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNAI1 ( NM_174324.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776749.1 Bos taurus G protein subunit alpha i1 (GNAI1), mRNA.

Price & Availability↑

CloneID	OBa41324
Clone ID Related Accession (Same CDS sequence)	NM_174324.2 , XM_015468832.1 , XM_015468832.2
Accession Version	NM_174324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X03642.1. On Jun 3, 2003 this sequence version replaced NM_174324.1. ##Evidence-Data-START## Transcript exon combination :: X03642.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTGTA CGCTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGGAACCTCC GCGAGGATGG CGAGAAGGCG GCGCGCGAGG TCAAGCTGCT GCTGCTCGGT 
GCTGGTGAAT CTGGGAAAAG TACAATTGTG AAGCAAATGA AAATTATCCA TGAAGCTGGT 
TATTCAGAAG AGGAATGTAA GCAGTACAAA GCTGTGGTCT ACAGTAACAC CATCCAGTCA 
ATTATCGCTA TCATTAGGGC CATGGGGAGA TTGAAGATTG ACTTCGGTGA CTCAGCCCGG 
GCGGATGATG CCCGCCAACT CTTTGTGCTT GCTGGCGCTG CAGAGGAAGG TTTTATGACT 
GCAGAACTTG CTGGAGTTAT AAAGAGACTT TGGAAAGACA GTGGTGTACA AGCCTGCTTC 
AACAGATCCC GAGAGTACCA GCTTAATGAT TCTGCAGCAT ACTATTTGAA TGATTTGGAC 
AGAATTGCAC AACCAAATTA TATTCCAACT CAACAAGATG TTCTCAGAAC TCGAGTGAAA 
ACCACAGGAA TTGTTGAGAC CCATTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAT 
GTGGGAGGAC AGAGATCTGA GCGGAAGAAA TGGATTCATT GCTTCGAAGG AGTGACCGCC 
ATCATCTTCT GTGTGGCGCT GAGTGACTAT GACCTGGTTC TAGCTGAAGA TGAAGAAATG 
AACCGAATGC ATGAAAGCAT GAAGTTATTC GACAGCATAT GTAACAACAA ATGGTTTACA 
GATACATCTA TTATACTTTT TCTGAACAAG AAGGATCTCT TTGAAGAAAA AATCAAGAAG 
AGCCCTCTCA CTATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCAGCT 
GCGTACATTC AGTGTCAGTT TGAAGACCTC AATAAGAGAA AGGACACAAA GGAAATATAC 
ACCCACTTCA CGTGCGCCAC GGACACCAAG AACGTGCAGT TCGTCTTTGA TGCCGTAACA 
GACGTCATCA TAAAAAATAA CCTAAAAGAC TGTGGTCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776749.1
CDS	117..1181
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	NM_174324.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha i1 (GNAI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174324.2

ORF Insert Sequence:

ATGGGCTGTA CGCTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGGAACCTCC GCGAGGATGG CGAGAAGGCG GCGCGCGAGG TCAAGCTGCT GCTGCTCGGT 
GCTGGTGAAT CTGGGAAAAG TACAATTGTG AAGCAAATGA AAATTATCCA TGAAGCTGGT 
TATTCAGAAG AGGAATGTAA GCAGTACAAA GCTGTGGTCT ACAGTAACAC CATCCAGTCA 
ATTATCGCTA TCATTAGGGC CATGGGGAGA TTGAAGATTG ACTTCGGTGA CTCAGCCCGG 
GCGGATGATG CCCGCCAACT CTTTGTGCTT GCTGGCGCTG CAGAGGAAGG TTTTATGACT 
GCAGAACTTG CTGGAGTTAT AAAGAGACTT TGGAAAGACA GTGGTGTACA AGCCTGCTTC 
AACAGATCCC GAGAGTACCA GCTTAATGAT TCTGCAGCAT ACTATTTGAA TGATTTGGAC 
AGAATTGCAC AACCAAATTA TATTCCAACT CAACAAGATG TTCTCAGAAC TCGAGTGAAA 
ACCACAGGAA TTGTTGAGAC CCATTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAT 
GTGGGAGGAC AGAGATCTGA GCGGAAGAAA TGGATTCATT GCTTCGAAGG AGTGACCGCC 
ATCATCTTCT GTGTGGCGCT GAGTGACTAT GACCTGGTTC TAGCTGAAGA TGAAGAAATG 
AACCGAATGC ATGAAAGCAT GAAGTTATTC GACAGCATAT GTAACAACAA ATGGTTTACA 
GATACATCTA TTATACTTTT TCTGAACAAG AAGGATCTCT TTGAAGAAAA AATCAAGAAG 
AGCCCTCTCA CTATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCAGCT 
GCGTACATTC AGTGTCAGTT TGAAGACCTC AATAAGAGAA AGGACACAAA GGAAATATAC 
ACCCACTTCA CGTGCGCCAC GGACACCAAG AACGTGCAGT TCGTCTTTGA TGCCGTAACA 
GACGTCATCA TAAAAAATAA CCTAAAAGAC TGTGGTCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAI1 ( XM_005205328.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205385.1 Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18489
Clone ID Related Accession (Same CDS sequence)	XM_005205328.3 , XM_005205328.4
Accession Version	XM_005205328.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205328.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAC GCCCAGGACT GGTTTTTTTT CGGTTTCCAA CTTTGGCAGC TCTCCCACCC 
GCACCCCAAG GGGCAGGTCC GCACACATCC CTGGGTGTGA GTAAACGGGA AGAAATTATC 
ACACTCTCGA GTAGGAAGCC AGCGCGCCGG ACTTCGAGGG AGCGGCAGCC AGCTTTCGCT 
CCTGGCACAA TGGGCTGTAC GCTGAGCGCC GAGGACAAGG CGGCGGTGGA GCGGAGTAAG 
ATGATCGACC GGAACCTCCG CGAGGATGGC GAGAAGGCGG CGCGCGAGGT CAAGCTGCTG 
CTGCTCGGTG CTGGTGAATC TGGGAAAAGT ACAATTGTGA AGCAAATGAA AATTATCCAT 
GAAGCTGGTT ATTCAGAAGA GGAATGTAAG CAGTACAAAG CTGTGGTCTA CAGTAACACC 
ATCCAGTCAA TTATCGCTAT CATTAGGGCC ATGGGGAGAT TGAAGATTGA CTTCGGTGAC 
TCAGCCCGGG CGGATGATGC CCGCCAACTC TTTGTGCTTG CTGGCGCTGC AGAGGAAGGT 
TTTATGACTG CAGAACTTGC TGGAGTTATA AAGAGACTTT GGAAAGACAG TGGTGTACAA 
GCCTGCTTCA ACAGATCCCG AGAGTACCAG CTTAATGATT CTGCAGCATA CTATTTGAAT 
GATTTGGACA GAATTGCACA ACCAAATTAT ATTCCAACTC AACAAGATGT TCTCAGAACT 
CGAGTGAAAA CCACAGGAAT TGTTGAGACC CATTTTACTT TCAAAGATCT TCATTTTAAA 
ATGTTTGATG TGGGAGGACA GAGATCTGAG CGGAAGAAAT GGATTCATTG CTTCGAAGGA 
GTGACCGCCA TCATCTTCTG TGTGGCGCTG AGTGACTATG ACCTGGTTCT AGCTGAAGAT 
GAAGAAATGA ACCGAATGCA TGAAAGCATG AAGTTATTCG ACAGCATATG TAACAACAAA 
TGGTTTACAG ATACATCTAT TATACTTTTT CTGAACAAGA AGGATCTCTT TGAAGAAAAA 
ATCAAGAAGA GCCCTCTCAC TATATGCTAT CCAGAATATG CAGGCTCAAA CACATATGAA 
GAGGCAGCTG CGTACATTCA GTGTCAGTTT GAAGACCTCA ATAAGAGAAA GGACACAAAG 
GAAATATACA CCCACTTCAC GTGCGCCACG GACACCAAGA ACGTGCAGTT CGTCTTTGAT 
GCCGTAACAG ACGTCATCAT AAAAAATAAC CTAAAAGACT GTGGTCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205385.1
CDS	96..1349
Translation	MLKRPGLVFFRFPTLAALPPAPQGAGPHTSLGVSKREEIITLSSRKPARRTSRERQPAFAPGTMGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_005205328.3
Organism	Bos taurus(cattle)
Definition	Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205328.3

ORF Insert Sequence:

ATGCTCAAAC GCCCAGGACT GGTTTTTTTT CGGTTTCCAA CTTTGGCAGC TCTCCCACCC 
GCACCCCAAG GGGCAGGTCC GCACACATCC CTGGGTGTGA GTAAACGGGA AGAAATTATC 
ACACTCTCGA GTAGGAAGCC AGCGCGCCGG ACTTCGAGGG AGCGGCAGCC AGCTTTCGCT 
CCTGGCACAA TGGGCTGTAC GCTGAGCGCC GAGGACAAGG CGGCGGTGGA GCGGAGTAAG 
ATGATCGACC GGAACCTCCG CGAGGATGGC GAGAAGGCGG CGCGCGAGGT CAAGCTGCTG 
CTGCTCGGTG CTGGTGAATC TGGGAAAAGT ACAATTGTGA AGCAAATGAA AATTATCCAT 
GAAGCTGGTT ATTCAGAAGA GGAATGTAAG CAGTACAAAG CTGTGGTCTA CAGTAACACC 
ATCCAGTCAA TTATCGCTAT CATTAGGGCC ATGGGGAGAT TGAAGATTGA CTTCGGTGAC 
TCAGCCCGGG CGGATGATGC CCGCCAACTC TTTGTGCTTG CTGGCGCTGC AGAGGAAGGT 
TTTATGACTG CAGAACTTGC TGGAGTTATA AAGAGACTTT GGAAAGACAG TGGTGTACAA 
GCCTGCTTCA ACAGATCCCG AGAGTACCAG CTTAATGATT CTGCAGCATA CTATTTGAAT 
GATTTGGACA GAATTGCACA ACCAAATTAT ATTCCAACTC AACAAGATGT TCTCAGAACT 
CGAGTGAAAA CCACAGGAAT TGTTGAGACC CATTTTACTT TCAAAGATCT TCATTTTAAA 
ATGTTTGATG TGGGAGGACA GAGATCTGAG CGGAAGAAAT GGATTCATTG CTTCGAAGGA 
GTGACCGCCA TCATCTTCTG TGTGGCGCTG AGTGACTATG ACCTGGTTCT AGCTGAAGAT 
GAAGAAATGA ACCGAATGCA TGAAAGCATG AAGTTATTCG ACAGCATATG TAACAACAAA 
TGGTTTACAG ATACATCTAT TATACTTTTT CTGAACAAGA AGGATCTCTT TGAAGAAAAA 
ATCAAGAAGA GCCCTCTCAC TATATGCTAT CCAGAATATG CAGGCTCAAA CACATATGAA 
GAGGCAGCTG CGTACATTCA GTGTCAGTTT GAAGACCTCA ATAAGAGAAA GGACACAAAG 
GAAATATACA CCCACTTCAC GTGCGCCACG GACACCAAGA ACGTGCAGTT CGTCTTTGAT 
GCCGTAACAG ACGTCATCAT AAAAAATAAC CTAAAAGACT GTGGTCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAI1 ( XM_005205328.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205385.1 Bos taurus G protein subunit alpha i1 (GNAI1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18489
Clone ID Related Accession (Same CDS sequence)	XM_005205328.3 , XM_005205328.4
Accession Version	XM_005205328.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205328.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAC GCCCAGGACT GGTTTTTTTT CGGTTTCCAA CTTTGGCAGC TCTCCCACCC 
GCACCCCAAG GGGCAGGTCC GCACACATCC CTGGGTGTGA GTAAACGGGA AGAAATTATC 
ACACTCTCGA GTAGGAAGCC AGCGCGCCGG ACTTCGAGGG AGCGGCAGCC AGCTTTCGCT 
CCTGGCACAA TGGGCTGTAC GCTGAGCGCC GAGGACAAGG CGGCGGTGGA GCGGAGTAAG 
ATGATCGACC GGAACCTCCG CGAGGATGGC GAGAAGGCGG CGCGCGAGGT CAAGCTGCTG 
CTGCTCGGTG CTGGTGAATC TGGGAAAAGT ACAATTGTGA AGCAAATGAA AATTATCCAT 
GAAGCTGGTT ATTCAGAAGA GGAATGTAAG CAGTACAAAG CTGTGGTCTA CAGTAACACC 
ATCCAGTCAA TTATCGCTAT CATTAGGGCC ATGGGGAGAT TGAAGATTGA CTTCGGTGAC 
TCAGCCCGGG CGGATGATGC CCGCCAACTC TTTGTGCTTG CTGGCGCTGC AGAGGAAGGT 
TTTATGACTG CAGAACTTGC TGGAGTTATA AAGAGACTTT GGAAAGACAG TGGTGTACAA 
GCCTGCTTCA ACAGATCCCG AGAGTACCAG CTTAATGATT CTGCAGCATA CTATTTGAAT 
GATTTGGACA GAATTGCACA ACCAAATTAT ATTCCAACTC AACAAGATGT TCTCAGAACT 
CGAGTGAAAA CCACAGGAAT TGTTGAGACC CATTTTACTT TCAAAGATCT TCATTTTAAA 
ATGTTTGATG TGGGAGGACA GAGATCTGAG CGGAAGAAAT GGATTCATTG CTTCGAAGGA 
GTGACCGCCA TCATCTTCTG TGTGGCGCTG AGTGACTATG ACCTGGTTCT AGCTGAAGAT 
GAAGAAATGA ACCGAATGCA TGAAAGCATG AAGTTATTCG ACAGCATATG TAACAACAAA 
TGGTTTACAG ATACATCTAT TATACTTTTT CTGAACAAGA AGGATCTCTT TGAAGAAAAA 
ATCAAGAAGA GCCCTCTCAC TATATGCTAT CCAGAATATG CAGGCTCAAA CACATATGAA 
GAGGCAGCTG CGTACATTCA GTGTCAGTTT GAAGACCTCA ATAAGAGAAA GGACACAAAG 
GAAATATACA CCCACTTCAC GTGCGCCACG GACACCAAGA ACGTGCAGTT CGTCTTTGAT 
GCCGTAACAG ACGTCATCAT AAAAAATAAC CTAAAAGACT GTGGTCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205385.1
CDS	112..1365
Translation	MLKRPGLVFFRFPTLAALPPAPQGAGPHTSLGVSKREEIITLSSRKPARRTSRERQPAFAPGTMGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_005205328.4
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha i1 (GNAI1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205328.4

ORF Insert Sequence:

ATGCTCAAAC GCCCAGGACT GGTTTTTTTT CGGTTTCCAA CTTTGGCAGC TCTCCCACCC 
GCACCCCAAG GGGCAGGTCC GCACACATCC CTGGGTGTGA GTAAACGGGA AGAAATTATC 
ACACTCTCGA GTAGGAAGCC AGCGCGCCGG ACTTCGAGGG AGCGGCAGCC AGCTTTCGCT 
CCTGGCACAA TGGGCTGTAC GCTGAGCGCC GAGGACAAGG CGGCGGTGGA GCGGAGTAAG 
ATGATCGACC GGAACCTCCG CGAGGATGGC GAGAAGGCGG CGCGCGAGGT CAAGCTGCTG 
CTGCTCGGTG CTGGTGAATC TGGGAAAAGT ACAATTGTGA AGCAAATGAA AATTATCCAT 
GAAGCTGGTT ATTCAGAAGA GGAATGTAAG CAGTACAAAG CTGTGGTCTA CAGTAACACC 
ATCCAGTCAA TTATCGCTAT CATTAGGGCC ATGGGGAGAT TGAAGATTGA CTTCGGTGAC 
TCAGCCCGGG CGGATGATGC CCGCCAACTC TTTGTGCTTG CTGGCGCTGC AGAGGAAGGT 
TTTATGACTG CAGAACTTGC TGGAGTTATA AAGAGACTTT GGAAAGACAG TGGTGTACAA 
GCCTGCTTCA ACAGATCCCG AGAGTACCAG CTTAATGATT CTGCAGCATA CTATTTGAAT 
GATTTGGACA GAATTGCACA ACCAAATTAT ATTCCAACTC AACAAGATGT TCTCAGAACT 
CGAGTGAAAA CCACAGGAAT TGTTGAGACC CATTTTACTT TCAAAGATCT TCATTTTAAA 
ATGTTTGATG TGGGAGGACA GAGATCTGAG CGGAAGAAAT GGATTCATTG CTTCGAAGGA 
GTGACCGCCA TCATCTTCTG TGTGGCGCTG AGTGACTATG ACCTGGTTCT AGCTGAAGAT 
GAAGAAATGA ACCGAATGCA TGAAAGCATG AAGTTATTCG ACAGCATATG TAACAACAAA 
TGGTTTACAG ATACATCTAT TATACTTTTT CTGAACAAGA AGGATCTCTT TGAAGAAAAA 
ATCAAGAAGA GCCCTCTCAC TATATGCTAT CCAGAATATG CAGGCTCAAA CACATATGAA 
GAGGCAGCTG CGTACATTCA GTGTCAGTTT GAAGACCTCA ATAAGAGAAA GGACACAAAG 
GAAATATACA CCCACTTCAC GTGCGCCACG GACACCAAGA ACGTGCAGTT CGTCTTTGAT 
GCCGTAACAG ACGTCATCAT AAAAAATAAC CTAAAAGACT GTGGTCTCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAI1 ( XM_015468832.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324318.1 Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X3, mRNA.

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CloneID	OBa41324
Clone ID Related Accession (Same CDS sequence)	NM_174324.2 , XM_015468832.1 , XM_015468832.2
Accession Version	XM_015468832.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTA CGCTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGGAACCTCC GCGAGGATGG CGAGAAGGCG GCGCGCGAGG TCAAGCTGCT GCTGCTCGGT 
GCTGGTGAAT CTGGGAAAAG TACAATTGTG AAGCAAATGA AAATTATCCA TGAAGCTGGT 
TATTCAGAAG AGGAATGTAA GCAGTACAAA GCTGTGGTCT ACAGTAACAC CATCCAGTCA 
ATTATCGCTA TCATTAGGGC CATGGGGAGA TTGAAGATTG ACTTCGGTGA CTCAGCCCGG 
GCGGATGATG CCCGCCAACT CTTTGTGCTT GCTGGCGCTG CAGAGGAAGG TTTTATGACT 
GCAGAACTTG CTGGAGTTAT AAAGAGACTT TGGAAAGACA GTGGTGTACA AGCCTGCTTC 
AACAGATCCC GAGAGTACCA GCTTAATGAT TCTGCAGCAT ACTATTTGAA TGATTTGGAC 
AGAATTGCAC AACCAAATTA TATTCCAACT CAACAAGATG TTCTCAGAAC TCGAGTGAAA 
ACCACAGGAA TTGTTGAGAC CCATTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAT 
GTGGGAGGAC AGAGATCTGA GCGGAAGAAA TGGATTCATT GCTTCGAAGG AGTGACCGCC 
ATCATCTTCT GTGTGGCGCT GAGTGACTAT GACCTGGTTC TAGCTGAAGA TGAAGAAATG 
AACCGAATGC ATGAAAGCAT GAAGTTATTC GACAGCATAT GTAACAACAA ATGGTTTACA 
GATACATCTA TTATACTTTT TCTGAACAAG AAGGATCTCT TTGAAGAAAA AATCAAGAAG 
AGCCCTCTCA CTATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCAGCT 
GCGTACATTC AGTGTCAGTT TGAAGACCTC AATAAGAGAA AGGACACAAA GGAAATATAC 
ACCCACTTCA CGTGCGCCAC GGACACCAAG AACGTGCAGT TCGTCTTTGA TGCCGTAACA 
GACGTCATCA TAAAAAATAA CCTAAAAGAC TGTGGTCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324318.1
CDS	150..1214
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_015468832.1
Organism	Bos taurus(cattle)
Definition	Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468832.1

ORF Insert Sequence:

ATGGGCTGTA CGCTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGGAACCTCC GCGAGGATGG CGAGAAGGCG GCGCGCGAGG TCAAGCTGCT GCTGCTCGGT 
GCTGGTGAAT CTGGGAAAAG TACAATTGTG AAGCAAATGA AAATTATCCA TGAAGCTGGT 
TATTCAGAAG AGGAATGTAA GCAGTACAAA GCTGTGGTCT ACAGTAACAC CATCCAGTCA 
ATTATCGCTA TCATTAGGGC CATGGGGAGA TTGAAGATTG ACTTCGGTGA CTCAGCCCGG 
GCGGATGATG CCCGCCAACT CTTTGTGCTT GCTGGCGCTG CAGAGGAAGG TTTTATGACT 
GCAGAACTTG CTGGAGTTAT AAAGAGACTT TGGAAAGACA GTGGTGTACA AGCCTGCTTC 
AACAGATCCC GAGAGTACCA GCTTAATGAT TCTGCAGCAT ACTATTTGAA TGATTTGGAC 
AGAATTGCAC AACCAAATTA TATTCCAACT CAACAAGATG TTCTCAGAAC TCGAGTGAAA 
ACCACAGGAA TTGTTGAGAC CCATTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAT 
GTGGGAGGAC AGAGATCTGA GCGGAAGAAA TGGATTCATT GCTTCGAAGG AGTGACCGCC 
ATCATCTTCT GTGTGGCGCT GAGTGACTAT GACCTGGTTC TAGCTGAAGA TGAAGAAATG 
AACCGAATGC ATGAAAGCAT GAAGTTATTC GACAGCATAT GTAACAACAA ATGGTTTACA 
GATACATCTA TTATACTTTT TCTGAACAAG AAGGATCTCT TTGAAGAAAA AATCAAGAAG 
AGCCCTCTCA CTATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCAGCT 
GCGTACATTC AGTGTCAGTT TGAAGACCTC AATAAGAGAA AGGACACAAA GGAAATATAC 
ACCCACTTCA CGTGCGCCAC GGACACCAAG AACGTGCAGT TCGTCTTTGA TGCCGTAACA 
GACGTCATCA TAAAAAATAA CCTAAAAGAC TGTGGTCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAI1 ( XM_015468832.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324318.1 Bos taurus G protein subunit alpha i1 (GNAI1), transcript variant X2, mRNA.

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CloneID	OBa41324
Clone ID Related Accession (Same CDS sequence)	NM_174324.2 , XM_015468832.1 , XM_015468832.2
Accession Version	XM_015468832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015468832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTA CGCTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGGAACCTCC GCGAGGATGG CGAGAAGGCG GCGCGCGAGG TCAAGCTGCT GCTGCTCGGT 
GCTGGTGAAT CTGGGAAAAG TACAATTGTG AAGCAAATGA AAATTATCCA TGAAGCTGGT 
TATTCAGAAG AGGAATGTAA GCAGTACAAA GCTGTGGTCT ACAGTAACAC CATCCAGTCA 
ATTATCGCTA TCATTAGGGC CATGGGGAGA TTGAAGATTG ACTTCGGTGA CTCAGCCCGG 
GCGGATGATG CCCGCCAACT CTTTGTGCTT GCTGGCGCTG CAGAGGAAGG TTTTATGACT 
GCAGAACTTG CTGGAGTTAT AAAGAGACTT TGGAAAGACA GTGGTGTACA AGCCTGCTTC 
AACAGATCCC GAGAGTACCA GCTTAATGAT TCTGCAGCAT ACTATTTGAA TGATTTGGAC 
AGAATTGCAC AACCAAATTA TATTCCAACT CAACAAGATG TTCTCAGAAC TCGAGTGAAA 
ACCACAGGAA TTGTTGAGAC CCATTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAT 
GTGGGAGGAC AGAGATCTGA GCGGAAGAAA TGGATTCATT GCTTCGAAGG AGTGACCGCC 
ATCATCTTCT GTGTGGCGCT GAGTGACTAT GACCTGGTTC TAGCTGAAGA TGAAGAAATG 
AACCGAATGC ATGAAAGCAT GAAGTTATTC GACAGCATAT GTAACAACAA ATGGTTTACA 
GATACATCTA TTATACTTTT TCTGAACAAG AAGGATCTCT TTGAAGAAAA AATCAAGAAG 
AGCCCTCTCA CTATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCAGCT 
GCGTACATTC AGTGTCAGTT TGAAGACCTC AATAAGAGAA AGGACACAAA GGAAATATAC 
ACCCACTTCA CGTGCGCCAC GGACACCAAG AACGTGCAGT TCGTCTTTGA TGCCGTAACA 
GACGTCATCA TAAAAAATAA CCTAAAAGAC TGTGGTCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324318.1
CDS	184..1248
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	XM_015468832.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein subunit alpha i1 (GNAI1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468832.2

ORF Insert Sequence:

ATGGGCTGTA CGCTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGGAACCTCC GCGAGGATGG CGAGAAGGCG GCGCGCGAGG TCAAGCTGCT GCTGCTCGGT 
GCTGGTGAAT CTGGGAAAAG TACAATTGTG AAGCAAATGA AAATTATCCA TGAAGCTGGT 
TATTCAGAAG AGGAATGTAA GCAGTACAAA GCTGTGGTCT ACAGTAACAC CATCCAGTCA 
ATTATCGCTA TCATTAGGGC CATGGGGAGA TTGAAGATTG ACTTCGGTGA CTCAGCCCGG 
GCGGATGATG CCCGCCAACT CTTTGTGCTT GCTGGCGCTG CAGAGGAAGG TTTTATGACT 
GCAGAACTTG CTGGAGTTAT AAAGAGACTT TGGAAAGACA GTGGTGTACA AGCCTGCTTC 
AACAGATCCC GAGAGTACCA GCTTAATGAT TCTGCAGCAT ACTATTTGAA TGATTTGGAC 
AGAATTGCAC AACCAAATTA TATTCCAACT CAACAAGATG TTCTCAGAAC TCGAGTGAAA 
ACCACAGGAA TTGTTGAGAC CCATTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAT 
GTGGGAGGAC AGAGATCTGA GCGGAAGAAA TGGATTCATT GCTTCGAAGG AGTGACCGCC 
ATCATCTTCT GTGTGGCGCT GAGTGACTAT GACCTGGTTC TAGCTGAAGA TGAAGAAATG 
AACCGAATGC ATGAAAGCAT GAAGTTATTC GACAGCATAT GTAACAACAA ATGGTTTACA 
GATACATCTA TTATACTTTT TCTGAACAAG AAGGATCTCT TTGAAGAAAA AATCAAGAAG 
AGCCCTCTCA CTATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCAGCT 
GCGTACATTC AGTGTCAGTT TGAAGACCTC AATAAGAGAA AGGACACAAA GGAAATATAC 
ACCCACTTCA CGTGCGCCAC GGACACCAAG AACGTGCAGT TCGTCTTTGA TGCCGTAACA 
GACGTCATCA TAAAAAATAA CCTAAAAGAC TGTGGTCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNAI1 ( XM_015468831.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324317.1 Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa90593
Clone ID Related Accession (Same CDS sequence)	XM_015468831.1
Accession Version	XM_015468831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103891.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAC GCCCAGGACT GGTTTTTTTT CGGTTTCCAA CTTTGGCAGC TCTCCCACCC 
GCACCCCAAG GGGCAGGTCC GCACACATCC CTGGGTGTGA GTAAACGGGA AGAAATTATC 
ACACTCTCGA GTAGGAAGCC AGCGCGCCGG ACTTCGAGGG AGCGGCAGCC AGCTTTCGCT 
CCTGGCACAA TGGGCTGTAC GCTGAGCGCC GAGGACAAGG CGGCGGTGGA GCGGAGTAAG 
ATGATCGACC GGAACCTCCG CGAGGATGGC GAGAAGGCGG CGCGCGAGGT CAAGCTGCTG 
CTGCTCGGTG CTGGTGAATC TGGGAAAAGT ACAATTGTGA AGCAAATGAA AATTATCCAT 
GAAGCTGGTT ATTCAGAAGA GGAATGTAAG CAGTACAAAG CTGTGGTCTA CAGTAACACC 
ATCCAGTCAA TTATCGCTAT CATTAGGGCC ATGGGGAGAT TGAAGATTGA CTTCGGTGAC 
TCAGCCCGGG CGGATGATGC CCGCCAACTC TTTGTGCTTG CTGGCGCTGC AGAGGAAGGT 
TTTATGACTG CAGAACTTGC TGGAGTTATA AAGAGACTTT GGAAAGACAG TGGTGTACAA 
GCCTGCTTCA ACAGATCCCG AGAGTACCAG CTTAATGATT CTGCAGCATA CTATTTGAAT 
GATTTGGACA GAATTGCACA ACCAAATTAT ATTCCAACTC AACAAGATGT TCTCAGAACT 
CGAGTGAAAA CCACAGGAAT TGTTGAGACC CATTTTACTT TCAAAGATCT TCATTTTAAA 
ATGTTTGATG TGGGAGGACA GAGATCTGAG CGGAAGAAAT GGATTCATTG CTTCGAAGGA 
GTGACCGCCA TCATCTTCTG TGTGGCGCTG AGTGACTATG ACCTGGTTCT AGCTGAAGAT 
GAAGAAATGA ACCGAATGCA TGAAAGCATG AAGTTATTCG ACAGCATATG TAACAACAAA 
TGGTTTACAG ATACATCTAT TATACTTTTT CTGAACAAGA AGGATCTCTT TGAAGAAAAA 
ATCAAGAAGA GCCCTCTCAC TATATGCTAT CCAGAATATG CAGACAGAGG TACTCCTAAG 
GAAGCAGATA AAACACCACC AGTGCAGCAT GTGACCACAG AGGAAAACGA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324317.1
CDS	109..1245
Translation	MLKRPGLVFFRFPTLAALPPAPQGAGPHTSLGVSKREEIITLSSRKPARRTSRERQPAFAPGTMGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYADRGTPKEADKTPPVQHVTTEENEQ

Target ORF information:

RefSeq Version	XM_015468831.1
Organism	Bos taurus(cattle)
Definition	Bos taurus guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 (GNAI1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468831.1

ORF Insert Sequence:

ATGCTCAAAC GCCCAGGACT GGTTTTTTTT CGGTTTCCAA CTTTGGCAGC TCTCCCACCC 
GCACCCCAAG GGGCAGGTCC GCACACATCC CTGGGTGTGA GTAAACGGGA AGAAATTATC 
ACACTCTCGA GTAGGAAGCC AGCGCGCCGG ACTTCGAGGG AGCGGCAGCC AGCTTTCGCT 
CCTGGCACAA TGGGCTGTAC GCTGAGCGCC GAGGACAAGG CGGCGGTGGA GCGGAGTAAG 
ATGATCGACC GGAACCTCCG CGAGGATGGC GAGAAGGCGG CGCGCGAGGT CAAGCTGCTG 
CTGCTCGGTG CTGGTGAATC TGGGAAAAGT ACAATTGTGA AGCAAATGAA AATTATCCAT 
GAAGCTGGTT ATTCAGAAGA GGAATGTAAG CAGTACAAAG CTGTGGTCTA CAGTAACACC 
ATCCAGTCAA TTATCGCTAT CATTAGGGCC ATGGGGAGAT TGAAGATTGA CTTCGGTGAC 
TCAGCCCGGG CGGATGATGC CCGCCAACTC TTTGTGCTTG CTGGCGCTGC AGAGGAAGGT 
TTTATGACTG CAGAACTTGC TGGAGTTATA AAGAGACTTT GGAAAGACAG TGGTGTACAA 
GCCTGCTTCA ACAGATCCCG AGAGTACCAG CTTAATGATT CTGCAGCATA CTATTTGAAT 
GATTTGGACA GAATTGCACA ACCAAATTAT ATTCCAACTC AACAAGATGT TCTCAGAACT 
CGAGTGAAAA CCACAGGAAT TGTTGAGACC CATTTTACTT TCAAAGATCT TCATTTTAAA 
ATGTTTGATG TGGGAGGACA GAGATCTGAG CGGAAGAAAT GGATTCATTG CTTCGAAGGA 
GTGACCGCCA TCATCTTCTG TGTGGCGCTG AGTGACTATG ACCTGGTTCT AGCTGAAGAT 
GAAGAAATGA ACCGAATGCA TGAAAGCATG AAGTTATTCG ACAGCATATG TAACAACAAA 
TGGTTTACAG ATACATCTAT TATACTTTTT CTGAACAAGA AGGATCTCTT TGAAGAAAAA 
ATCAAGAAGA GCCCTCTCAC TATATGCTAT CCAGAATATG CAGACAGAGG TACTCCTAAG 
GAAGCAGATA AAACACCACC AGTGCAGCAT GTGACCACAG AGGAAAACGA ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes.
Proceedings of the National Academy of Sciences of the United States of America107(2)639-44(2010 Jan)
Smrcka AV,Kichik N,Tarrag? T,Burroughs M,Park MS,Itoga NK,Stern HA,Willardson BM,Giralt E

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Synteny mapping of five human chromosome 7 genes on bovine chromosomes 4 and 21.
Cytogenetics and cell genetics84(1-2)121-4(1999)
Antoniou E,Womack JE,Grosz MD

Molecular cloning and characterization of cDNA encoding the GTP-binding protein alpha i and identification of a related protein, alpha h.
Proceedings of the National Academy of Sciences of the United States of America83(20)7663-7(1986 Oct)
Michel T,Winslow JW,Smith JA,Seidman JG,Neer EJ

Primary structure of the alpha-subunit of bovine adenylate cyclase-inhibiting G-protein deduced from the cDNA sequence.
FEBS letters197(1-2)305-10(1986 Mar)
Nukada T,Tanabe T,Takahashi H,Noda M,Haga K,Haga T,Ichiyama A,Kanagawa K,Hiranaga M,Matsuo

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GNAI1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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