FABP4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	FABP4
Entrez Gene ID	281759
Full Name	fatty acid binding protein 4, adipocyte
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174314.2	NP_776739.1	fatty acid-binding protein, adipocyte

Pathways from BioSystems

Homologies

Homo sapiens (human)	FABP4	NP_001433.1
Gallus gallus (chicken)	FABP4	NP_989621.1
Pan troglodytes (chimpanzee)	FABP4	XP_519830.1
	FABP4	XP_001092255.1
Mus musculus (house mouse)	Fabp4	NP_077717.1
Rattus norvegicus (Norway rat)	Fabp4	NP_445817.1
Caenorhabditis elegans (roundworm)	lbp-8	NP_506444.2
Canis lupus familiaris (dog)	FABP4	XP_850162.1
Bos taurus (cattle)	FABP4	NP_776739.1
Xenopus tropicalis (tropical clawed frog)	pmp2	NP_001107544.1
Canis lupus familiaris (dog)	LOC100686507	XP_003435639.1
Caenorhabditis elegans (roundworm)	lbp-6	NP_491926.1
Caenorhabditis elegans (roundworm)	lbp-5	NP_491928.1
Caenorhabditis elegans (roundworm)	lbp-7	NP_506440.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content.
Goszczynski DE, Papaleo-Mazzucco J, Ripoli MV, Villarreal EL, Rogberg-Mu?oz A, Mezzadra CA, Melucci LM, Giovambattista G
Animal biotechnology28(3)211-219(2017 Jul)

Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes.
Yonekura S, Hirota S, Miyazaki H, Tokutake Y
Animal biotechnology27(2)96-103(2016)

Tissue expression analysis, cloning, and characterization of the 5'-regulatory region of the bovine fatty acid binding protein 4 gene.
Li A, Zhao Z, Zhang Y, Fu C, Wang M, Zan L
Journal of animal science93(11)5144-52(2015 Nov)

Variation in the bovine FABP4 gene affects milk yield and milk protein content in dairy cows.
Zhou H, Cheng L, Azimu W, Hodge S, Edwards GR, Hickford JG
Scientific reports510023(2015 Jun)

Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice.
Liu ZW, Fan HL, Liu XF, Ding XB, Wang T, Sui GN, Li GP, Guo H
Genetics and molecular research : GMR14(1)2742-9(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studied SNPs in cattle Fatty Acid Binding Protein 4 (FABP4) in several cattle breeds and evaluated possible associations with regards to meat quality and fat content of the meat.
Title: Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content.

Overexpressed FABP4 of different genotypes in bovine intramuscular preadipocytes and studied the intracellular localization of FABP4 and the expression levels of lipid metabolism-related genes among different genotypes.
Title: Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes.

These results provide an important basis for further understanding the regulation of bovine FABP4.
Title: Tissue expression analysis, cloning, and characterization of the 5'-regulatory region of the bovine fatty acid binding protein 4 gene.

This suggest that FABP4 affects milk yield and milk protein content, both economically important traits, and that further study of this gene is warranted.
Title: Variation in the bovine FABP4 gene affects milk yield and milk protein content in dairy cows.

Data show that overexpression of cattle adipocyte fatty acid-binding protein (A-FABP)gene promoted fat deposition in the skeletal muscle of transgenic mice.
Title: Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice.

The following FABP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FABP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01450	NM_174314.2 Latest version!	Bos taurus fatty acid binding protein 4, adipocyte (FABP4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FABP4 ( NM_174314.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776739.1 Bos taurus fatty acid binding protein 4, adipocyte (FABP4), mRNA.

Price & Availability↑

CloneID	OBa01450
Clone ID Related Accession (Same CDS sequence)	NM_174314.2
Accession Version	NM_174314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	fatty acid-binding protein, adipocyte
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X89244.1. On Jun 3, 2003 this sequence version replaced NM_174314.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X89244.1, FE019187.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145438 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGATG CATTTGTAGG TACCTGGAAA CTTGTCTCCA GTGAAAACTT TGATGATTAC 
ATGAAAGAAG TGGGCGTGGG CTTTGCTACC AGGAAAGTGG CTGGCATGGC CAAACCCACT 
TTGATCATCA GTTTGAATGG GGGTGTGGTC ACCATTAAAT CAGAAAGCAC CTTTAAAAAT 
ACTGAGATTT CCTTCAAATT GGGCCAGGAA TTTGATGAAA TCACTCCAGA TGACAGGAAA 
GTCAAGAGCA TCGTAAACTT AGATGAAGGT GCTCTGGTAC AAGTACAAAA CTGGGATGGA 
AAATCAACCA CCATAAAGAG AAAACTCATG GATGATAAGA TGGTGCTGGA ATGTGTCATG 
AATGGTGTCA CTGCCACCAG AGTTTATGAG AGAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776739.1
CDS	61..459
Translation	MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPTLIISLNGGVVTIKSESTFKNTEISFKLGQEFDEITPDDRKVKSIVNLDEGALVQVQNWDGKSTTIKRKLMDDKMVLECVMNGVTATRVYERA

Target ORF information:

RefSeq Version	NM_174314.2
Organism	Bos taurus(cattle)
Definition	Bos taurus fatty acid binding protein 4, adipocyte (FABP4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174314.2

ORF Insert Sequence:

ATGTGTGATG CATTTGTAGG TACCTGGAAA CTTGTCTCCA GTGAAAACTT TGATGATTAC 
ATGAAAGAAG TGGGCGTGGG CTTTGCTACC AGGAAAGTGG CTGGCATGGC CAAACCCACT 
TTGATCATCA GTTTGAATGG GGGTGTGGTC ACCATTAAAT CAGAAAGCAC CTTTAAAAAT 
ACTGAGATTT CCTTCAAATT GGGCCAGGAA TTTGATGAAA TCACTCCAGA TGACAGGAAA 
GTCAAGAGCA TCGTAAACTT AGATGAAGGT GCTCTGGTAC AAGTACAAAA CTGGGATGGA 
AAATCAACCA CCATAAAGAG AAAACTCATG GATGATAAGA TGGTGCTGGA ATGTGTCATG 
AATGGTGTCA CTGCCACCAG AGTTTATGAG AGAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content.
Animal biotechnology28(3)211-219(2017 Jul)
Goszczynski DE,Papaleo-Mazzucco J,Ripoli MV,Villarreal EL,Rogberg-Mu?oz A,Mezzadra CA,Melucci LM,Giovambattista G

Subcellular Localization and Polymorphism of Bovine FABP4 in Bovine Intramuscular Adipocytes.
Animal biotechnology27(2)96-103(2016)
Yonekura S,Hirota S,Miyazaki H,Tokutake Y

Tissue expression analysis, cloning, and characterization of the 5'-regulatory region of the bovine fatty acid binding protein 4 gene.
Journal of animal science93(11)5144-52(2015 Nov)
Li A,Zhao Z,Zhang Y,Fu C,Wang M,Zan L

Variation in the bovine FABP4 gene affects milk yield and milk protein content in dairy cows.
Scientific reports510023(2015 Jun)
Zhou H,Cheng L,Azimu W,Hodge S,Edwards GR,Hickford JG

Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice.
Genetics and molecular research : GMR14(1)2742-9(2015 Mar)
Liu ZW,Fan HL,Liu XF,Ding XB,Wang T,Sui GN,Li GP,Guo H

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FABP4 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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