ECHS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECHS1
Entrez Gene ID	281748
Full Name	enoyl-CoA hydratase, short chain 1
General protein information	Preferred Names enoyl-CoA hydratase, short chain 1 Names enoyl-CoA hydratase, mitochondrial SCEH enoyl CoA hydratase, short chain, 1, mitochondrial enoyl Coenzyme A hydratase, short chain, 1, mitochondrial enoyl-CoA hydratase 1 short-chain enoyl-CoA hydratase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025206.2	NP_001020377.2	enoyl-CoA hydratase, mitochondrial precursor
XM_015460602.1	XP_015316088.1	enoyl-CoA hydratase, mitochondrial isoform X1
XM_015460602.2	XP_015316088.1	enoyl-CoA hydratase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Echs1	NP_511178.1
Homo sapiens (human)	ECHS1	NP_004083.3
Rattus norvegicus (Norway rat)	LOC100911186	XP_003748934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011833	XP_320683.2
Caenorhabditis elegans (roundworm)	ech-6	NP_499156.1
Xenopus tropicalis (tropical clawed frog)	echs1	NP_001005437.1
Canis lupus familiaris (dog)	ECHS1	XP_537945.4
Danio rerio (zebrafish)	echs1	NP_001004529.1
Drosophila melanogaster (fruit fly)	CG6543	NP_610910.1
Neurospora crassa	NCU06448	XP_957339.1
Gallus gallus (chicken)	ECHS1	NP_001264324.1
Pan troglodytes (chimpanzee)	ECHS1	XP_003312886.1
Bos taurus (cattle)	ECHS1	NP_001020377.2
Mus musculus (house mouse)	Echs1	NP_444349.1
Magnaporthe oryzae (rice blast fungus)	MGG_12868	XP_003717318.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cloning of the bovine prion-like Shadoo (SPRN) gene by comparative analysis of the predicted genomic locus.
Uboldi C, Paulis M, Guidi E, Bertoni A, Meo GP, Perucatti A, Iannuzzi L, Raimondi E, Brunner RM, Eggen A, Ferretti L
Mammalian genome : official journal of the International Mammalian Genome Society17(11)1130-9(2006 Nov)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ECHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43550	NM_001025206.2 Latest version!	Bos taurus enoyl-CoA hydratase, short chain 1 (ECHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa88645	XM_015460602.2 Latest version!	Bos taurus enoyl-CoA hydratase, short chain 1 (ECHS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OBa88645	XM_015460602.1	Bos taurus enoyl-CoA hydratase, short chain, 1, mitochondrial (ECHS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECHS1 ( NM_001025206.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001020377.2 Bos taurus enoyl-CoA hydratase, short chain 1 (ECHS1), mRNA.

Price & Availability↑

CloneID	OBa43550
Clone ID Related Accession (Same CDS sequence)	NM_001025206.2
Accession Version	NM_001025206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	enoyl-CoA hydratase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT021569.1. On Jul 13, 2005 this sequence version replaced NM_001025206.1. ##Evidence-Data-START## Transcript exon combination :: BT021569.1, DV928611.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCGCCT TACGTGCCCT GCTGCCCCGC GTCCGCGCCC CGCTGCGGCC CTGGCTCTTC 
TGCCCGGTGC AGCGCTCCTT CGCCTCGAGC GCAGCCTTTG AGTACATCAT CACAGCAAAG 
AAGGGAAGGA ACAGCAACGT GGGGTTGATC CAGCTGAACC GCCCCAAGGC CCTCAATGCG 
CTCTGCAACG GCCTGATCGT GGAGCTCAAC CAGGCACTGC AGGCCTTCGA GGAAGACCCA 
GCCGTGGGGG CCATTGTCCT CACAGGCGGG GAGAAGGTAT TTGCAGCTGG AGCCGACATC 
AAGGAAATGC AGAGCCTCAC GTTCCAGAAC TGTTACTCTG GCGGGTTTTT GAGCCACTGG 
GACCAGCTCA CACGTGTCAA GAAGCCAGTC ATAGCTGCTG TCAATGGCTA TGCCCTTGGT 
GGTGGCTGTG AACTTGCTAT GATGTGTGAC ATCATTTATG CTGGAGAGAA AGCCCAGTTT 
GGGCAGCCGG AGATTCTAAT AGGAACCATC CCAGGTGCCG GCGGCACCCA GAGACTGACC 
CGTGCCGTCG GAAAGTCACT GGCCATGGAG ATGGTCCTCA CTGGTGACCG GATCTCAGCC 
CAGGATGCCA AGCAAGCAGG TCTCGTAAGC AAAATTTTTC CTGTCGAGAC AGTGGTTGAA 
GAAGCCATCC AATGTGCAGA AAAAATTGCC AGCAACTCTA AAATTGTAAC AGCCATGGCC 
AAAGAATCAG TGAACGCAGC TTTTGAAATG ACATTAGCTG AGGGCGTTAA ATTGGAGAAG 
AAGCTCTTCT ACTCAACTTT TGCCACCGAA GACCGGAAGG AAGGCATGGC CGCGTTTGTG 
GAGAAGAGAA AGGCCAACTT CAAAGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020377.2
CDS	26..898
Translation	MAALRALLPRVRAPLRPWLFCPVQRSFASSAAFEYIITAKKGRNSNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTGGEKVFAAGADIKEMQSLTFQNCYSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFEMTLAEGVKLEKKLFYSTFATEDRKEGMAAFVEKRKANFKDQ

Target ORF information:

RefSeq Version	NM_001025206.2
Organism	Bos taurus(cattle)
Definition	Bos taurus enoyl-CoA hydratase, short chain 1 (ECHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025206.2

ORF Insert Sequence:

ATGGCCGCCT TACGTGCCCT GCTGCCCCGC GTCCGCGCCC CGCTGCGGCC CTGGCTCTTC 
TGCCCGGTGC AGCGCTCCTT CGCCTCGAGC GCAGCCTTTG AGTACATCAT CACAGCAAAG 
AAGGGAAGGA ACAGCAACGT GGGGTTGATC CAGCTGAACC GCCCCAAGGC CCTCAATGCG 
CTCTGCAACG GCCTGATCGT GGAGCTCAAC CAGGCACTGC AGGCCTTCGA GGAAGACCCA 
GCCGTGGGGG CCATTGTCCT CACAGGCGGG GAGAAGGTAT TTGCAGCTGG AGCCGACATC 
AAGGAAATGC AGAGCCTCAC GTTCCAGAAC TGTTACTCTG GCGGGTTTTT GAGCCACTGG 
GACCAGCTCA CACGTGTCAA GAAGCCAGTC ATAGCTGCTG TCAATGGCTA TGCCCTTGGT 
GGTGGCTGTG AACTTGCTAT GATGTGTGAC ATCATTTATG CTGGAGAGAA AGCCCAGTTT 
GGGCAGCCGG AGATTCTAAT AGGAACCATC CCAGGTGCCG GCGGCACCCA GAGACTGACC 
CGTGCCGTCG GAAAGTCACT GGCCATGGAG ATGGTCCTCA CTGGTGACCG GATCTCAGCC 
CAGGATGCCA AGCAAGCAGG TCTCGTAAGC AAAATTTTTC CTGTCGAGAC AGTGGTTGAA 
GAAGCCATCC AATGTGCAGA AAAAATTGCC AGCAACTCTA AAATTGTAAC AGCCATGGCC 
AAAGAATCAG TGAACGCAGC TTTTGAAATG ACATTAGCTG AGGGCGTTAA ATTGGAGAAG 
AAGCTCTTCT ACTCAACTTT TGCCACCGAA GACCGGAAGG AAGGCATGGC CGCGTTTGTG 
GAGAAGAGAA AGGCCAACTT CAAAGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECHS1 ( XM_015460602.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316088.1 Bos taurus enoyl-CoA hydratase, short chain, 1, mitochondrial (ECHS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88645
Clone ID Related Accession (Same CDS sequence)	XM_015460602.1 , XM_015460602.2
Accession Version	XM_015460602.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	enoyl-CoA hydratase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGTG TGGGGGATGC TGCCTACACA TGGGTGATTT CAGACACTCC CCAGCAAGCC 
CACCGGGTAG GTACCCCGTT CAAGGAAGCA GCTGTCTTGG TTAAGCTTCC CCCTACCAAG 
GTCATTCTGG CTCCCAGGAT TGAGCAGGCT GCCCCTCCTG GTCTGGCAGT TGCCCTTTGC 
CTGTGTGCCC TTCACCCAGT TGGCATGTTT AAAGAGGGAG TTTCCTCATT TCTTGTTTCT 
ACAGGATCCC AGGCGTCTCC AGCACAGGTT CGGGGAGATG CCAACCAAGA CATTCAGAAG 
GAAGGCTGCT GGTCATTGCT GGGGCTGTTG GGCTGTCTTA ACTTAAGGAA ACATTCCAAC 
ATCTGTGAAA GCAGAGAGCC CTGCAGAAGG CCCCCATGCA GTGCGAGCTG CAGCACCAGG 
GACTGGGCAG GGCCCTCAGG CGCAGCCTTT GAGTACATCA TCACAGCAAA GAAGGGAAGG 
AACAGCAACG TGGGGTTGAT CCAGCTGAAC CGCCCCAAGG CCCTCAATGC GCTCTGCAAC 
GGCCTGATCG TGGAGCTCAA CCAGGCACTG CAGGCCTTCG AGGAAGACCC AGCCGTGGGG 
GCCATTGTCC TCACAGGCGG GGAGAAGGTA TTTGCAGCTG GAGCCGACAT CAAGGAAATG 
CAGAGCCTCA CGTTCCAGAA CTGTTACTCT GGCGGGTTTT TGAGCCACTG GGACCAGCTC 
ACACGTGTCA AGAAGCCAGT CATAGCTGCT GTCAATGGCT ATGCCCTTGG TGGTGGCTGT 
GAACTTGCTA TGATGTGTGA CATCATTTAT GCTGGAGAGA AAGCCCAGTT TGGGCAGCCG 
GAGATTCTAA TAGGAACCAT CCCAGGTGCC GGCGGCACCC AGAGACTGAC CCGTGCCGTC 
GGAAAGTCAC TGGCCATGGA GATGGTCCTC ACTGGTGACC GGATCTCAGC CCAGGATGCC 
AAGCAAGCAG GTCTCGTAAG CAAAATTTTT CCTGTCGAGA CAGTGGTTGA AGAAGCCATC 
CAATGTGCAG AAAAAATTGC CAGCAACTCT AAAATTGTAA CAGCCATGGC CAAAGAATCA 
GTGAACGCAG CTTTTGAAAT GACATTAGCT GAGGGCGTTA AATTGGAGAA GAAGCTCTTC 
TACTCAACTT TTGCCACCGA AGACCGGAAG GAAGGCATGG CCGCGTTTGT GGAGAAGAGA 
AAGGCCAACT TCAAAGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316088.1
CDS	111..1334
Translation	MWGVGDAAYTWVISDTPQQAHRVGTPFKEAAVLVKLPPTKVILAPRIEQAAPPGLAVALCLCALHPVGMFKEGVSSFLVSTGSQASPAQVRGDANQDIQKEGCWSLLGLLGCLNLRKHSNICESREPCRRPPCSASCSTRDWAGPSGAAFEYIITAKKGRNSNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTGGEKVFAAGADIKEMQSLTFQNCYSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFEMTLAEGVKLEKKLFYSTFATEDRKEGMAAFVEKRKANFKDQ

Target ORF information:

RefSeq Version	XM_015460602.1
Organism	Bos taurus(cattle)
Definition	Bos taurus enoyl-CoA hydratase, short chain, 1, mitochondrial (ECHS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460602.1

ORF Insert Sequence:

ATGTGGGGTG TGGGGGATGC TGCCTACACA TGGGTGATTT CAGACACTCC CCAGCAAGCC 
CACCGGGTAG GTACCCCGTT CAAGGAAGCA GCTGTCTTGG TTAAGCTTCC CCCTACCAAG 
GTCATTCTGG CTCCCAGGAT TGAGCAGGCT GCCCCTCCTG GTCTGGCAGT TGCCCTTTGC 
CTGTGTGCCC TTCACCCAGT TGGCATGTTT AAAGAGGGAG TTTCCTCATT TCTTGTTTCT 
ACAGGATCCC AGGCGTCTCC AGCACAGGTT CGGGGAGATG CCAACCAAGA CATTCAGAAG 
GAAGGCTGCT GGTCATTGCT GGGGCTGTTG GGCTGTCTTA ACTTAAGGAA ACATTCCAAC 
ATCTGTGAAA GCAGAGAGCC CTGCAGAAGG CCCCCATGCA GTGCGAGCTG CAGCACCAGG 
GACTGGGCAG GGCCCTCAGG CGCAGCCTTT GAGTACATCA TCACAGCAAA GAAGGGAAGG 
AACAGCAACG TGGGGTTGAT CCAGCTGAAC CGCCCCAAGG CCCTCAATGC GCTCTGCAAC 
GGCCTGATCG TGGAGCTCAA CCAGGCACTG CAGGCCTTCG AGGAAGACCC AGCCGTGGGG 
GCCATTGTCC TCACAGGCGG GGAGAAGGTA TTTGCAGCTG GAGCCGACAT CAAGGAAATG 
CAGAGCCTCA CGTTCCAGAA CTGTTACTCT GGCGGGTTTT TGAGCCACTG GGACCAGCTC 
ACACGTGTCA AGAAGCCAGT CATAGCTGCT GTCAATGGCT ATGCCCTTGG TGGTGGCTGT 
GAACTTGCTA TGATGTGTGA CATCATTTAT GCTGGAGAGA AAGCCCAGTT TGGGCAGCCG 
GAGATTCTAA TAGGAACCAT CCCAGGTGCC GGCGGCACCC AGAGACTGAC CCGTGCCGTC 
GGAAAGTCAC TGGCCATGGA GATGGTCCTC ACTGGTGACC GGATCTCAGC CCAGGATGCC 
AAGCAAGCAG GTCTCGTAAG CAAAATTTTT CCTGTCGAGA CAGTGGTTGA AGAAGCCATC 
CAATGTGCAG AAAAAATTGC CAGCAACTCT AAAATTGTAA CAGCCATGGC CAAAGAATCA 
GTGAACGCAG CTTTTGAAAT GACATTAGCT GAGGGCGTTA AATTGGAGAA GAAGCTCTTC 
TACTCAACTT TTGCCACCGA AGACCGGAAG GAAGGCATGG CCGCGTTTGT GGAGAAGAGA 
AAGGCCAACT TCAAAGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECHS1 ( XM_015460602.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316088.1 Bos taurus enoyl-CoA hydratase, short chain 1 (ECHS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88645
Clone ID Related Accession (Same CDS sequence)	XM_015460602.1 , XM_015460602.2
Accession Version	XM_015460602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	enoyl-CoA hydratase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGTG TGGGGGATGC TGCCTACACA TGGGTGATTT CAGACACTCC CCAGCAAGCC 
CACCGGGTAG GTACCCCGTT CAAGGAAGCA GCTGTCTTGG TTAAGCTTCC CCCTACCAAG 
GTCATTCTGG CTCCCAGGAT TGAGCAGGCT GCCCCTCCTG GTCTGGCAGT TGCCCTTTGC 
CTGTGTGCCC TTCACCCAGT TGGCATGTTT AAAGAGGGAG TTTCCTCATT TCTTGTTTCT 
ACAGGATCCC AGGCGTCTCC AGCACAGGTT CGGGGAGATG CCAACCAAGA CATTCAGAAG 
GAAGGCTGCT GGTCATTGCT GGGGCTGTTG GGCTGTCTTA ACTTAAGGAA ACATTCCAAC 
ATCTGTGAAA GCAGAGAGCC CTGCAGAAGG CCCCCATGCA GTGCGAGCTG CAGCACCAGG 
GACTGGGCAG GGCCCTCAGG CGCAGCCTTT GAGTACATCA TCACAGCAAA GAAGGGAAGG 
AACAGCAACG TGGGGTTGAT CCAGCTGAAC CGCCCCAAGG CCCTCAATGC GCTCTGCAAC 
GGCCTGATCG TGGAGCTCAA CCAGGCACTG CAGGCCTTCG AGGAAGACCC AGCCGTGGGG 
GCCATTGTCC TCACAGGCGG GGAGAAGGTA TTTGCAGCTG GAGCCGACAT CAAGGAAATG 
CAGAGCCTCA CGTTCCAGAA CTGTTACTCT GGCGGGTTTT TGAGCCACTG GGACCAGCTC 
ACACGTGTCA AGAAGCCAGT CATAGCTGCT GTCAATGGCT ATGCCCTTGG TGGTGGCTGT 
GAACTTGCTA TGATGTGTGA CATCATTTAT GCTGGAGAGA AAGCCCAGTT TGGGCAGCCG 
GAGATTCTAA TAGGAACCAT CCCAGGTGCC GGCGGCACCC AGAGACTGAC CCGTGCCGTC 
GGAAAGTCAC TGGCCATGGA GATGGTCCTC ACTGGTGACC GGATCTCAGC CCAGGATGCC 
AAGCAAGCAG GTCTCGTAAG CAAAATTTTT CCTGTCGAGA CAGTGGTTGA AGAAGCCATC 
CAATGTGCAG AAAAAATTGC CAGCAACTCT AAAATTGTAA CAGCCATGGC CAAAGAATCA 
GTGAACGCAG CTTTTGAAAT GACATTAGCT GAGGGCGTTA AATTGGAGAA GAAGCTCTTC 
TACTCAACTT TTGCCACCGA AGACCGGAAG GAAGGCATGG CCGCGTTTGT GGAGAAGAGA 
AAGGCCAACT TCAAAGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316088.1
CDS	127..1350
Translation	MWGVGDAAYTWVISDTPQQAHRVGTPFKEAAVLVKLPPTKVILAPRIEQAAPPGLAVALCLCALHPVGMFKEGVSSFLVSTGSQASPAQVRGDANQDIQKEGCWSLLGLLGCLNLRKHSNICESREPCRRPPCSASCSTRDWAGPSGAAFEYIITAKKGRNSNVGLIQLNRPKALNALCNGLIVELNQALQAFEEDPAVGAIVLTGGEKVFAAGADIKEMQSLTFQNCYSGGFLSHWDQLTRVKKPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKIFPVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFEMTLAEGVKLEKKLFYSTFATEDRKEGMAAFVEKRKANFKDQ

Target ORF information:

RefSeq Version	XM_015460602.2
Organism	Bos taurus(cattle)
Definition	Bos taurus enoyl-CoA hydratase, short chain 1 (ECHS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460602.2

ORF Insert Sequence:

ATGTGGGGTG TGGGGGATGC TGCCTACACA TGGGTGATTT CAGACACTCC CCAGCAAGCC 
CACCGGGTAG GTACCCCGTT CAAGGAAGCA GCTGTCTTGG TTAAGCTTCC CCCTACCAAG 
GTCATTCTGG CTCCCAGGAT TGAGCAGGCT GCCCCTCCTG GTCTGGCAGT TGCCCTTTGC 
CTGTGTGCCC TTCACCCAGT TGGCATGTTT AAAGAGGGAG TTTCCTCATT TCTTGTTTCT 
ACAGGATCCC AGGCGTCTCC AGCACAGGTT CGGGGAGATG CCAACCAAGA CATTCAGAAG 
GAAGGCTGCT GGTCATTGCT GGGGCTGTTG GGCTGTCTTA ACTTAAGGAA ACATTCCAAC 
ATCTGTGAAA GCAGAGAGCC CTGCAGAAGG CCCCCATGCA GTGCGAGCTG CAGCACCAGG 
GACTGGGCAG GGCCCTCAGG CGCAGCCTTT GAGTACATCA TCACAGCAAA GAAGGGAAGG 
AACAGCAACG TGGGGTTGAT CCAGCTGAAC CGCCCCAAGG CCCTCAATGC GCTCTGCAAC 
GGCCTGATCG TGGAGCTCAA CCAGGCACTG CAGGCCTTCG AGGAAGACCC AGCCGTGGGG 
GCCATTGTCC TCACAGGCGG GGAGAAGGTA TTTGCAGCTG GAGCCGACAT CAAGGAAATG 
CAGAGCCTCA CGTTCCAGAA CTGTTACTCT GGCGGGTTTT TGAGCCACTG GGACCAGCTC 
ACACGTGTCA AGAAGCCAGT CATAGCTGCT GTCAATGGCT ATGCCCTTGG TGGTGGCTGT 
GAACTTGCTA TGATGTGTGA CATCATTTAT GCTGGAGAGA AAGCCCAGTT TGGGCAGCCG 
GAGATTCTAA TAGGAACCAT CCCAGGTGCC GGCGGCACCC AGAGACTGAC CCGTGCCGTC 
GGAAAGTCAC TGGCCATGGA GATGGTCCTC ACTGGTGACC GGATCTCAGC CCAGGATGCC 
AAGCAAGCAG GTCTCGTAAG CAAAATTTTT CCTGTCGAGA CAGTGGTTGA AGAAGCCATC 
CAATGTGCAG AAAAAATTGC CAGCAACTCT AAAATTGTAA CAGCCATGGC CAAAGAATCA 
GTGAACGCAG CTTTTGAAAT GACATTAGCT GAGGGCGTTA AATTGGAGAA GAAGCTCTTC 
TACTCAACTT TTGCCACCGA AGACCGGAAG GAAGGCATGG CCGCGTTTGT GGAGAAGAGA 
AAGGCCAACT TCAAAGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cloning of the bovine prion-like Shadoo (SPRN) gene by comparative analysis of the predicted genomic locus.
Mammalian genome : official journal of the International Mammalian Genome Society17(11)1130-9(2006 Nov)
Uboldi C,Paulis M,Guidi E,Bertoni A,Meo GP,Perucatti A,Iannuzzi L,Raimondi E,Brunner RM,Eggen A,Ferretti L

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

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ECHS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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