CPSF3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CPSF3
Entrez Gene ID	281712
Full Name	cleavage and polyadenylation specific factor 3
General protein information	Preferred Names cleavage and polyadenylation specific factor 3 Names cleavage and polyadenylation specificity factor subunit 3 CPSF 73 kDa subunit cleavage and polyadenylation specific factor 3, 73kDa cleavage and polyadenylation specificity factor 73 kDa subunit mRNA 3'-end-processing endonuclease CPSF-73
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473528.1	XP_015329014.1	cleavage and polyadenylation specificity factor subunit 3 isoform X1
NM_174284.2	NP_776709.1	cleavage and polyadenylation specificity factor subunit 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CPSF3	NP_057291.1
Caenorhabditis elegans (roundworm)	cpsf-3	NP_502553.2
Oryza sativa (rice)	Os03g0852900	NP_001051928.1
Canis lupus familiaris (dog)	CPSF3	XP_005630164.1
Mus musculus (house mouse)	Cpsf3	NP_061283.2
Danio rerio (zebrafish)	cpsf3	NP_001003836.1
Kluyveromyces lactis	KLLA0C04598g	XP_452404.1
Eremothecium gossypii	AGOS_AGR279C	NP_986945.2
Magnaporthe oryzae (rice blast fungus)	MGG_01804	XP_003714815.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001224	XP_321933.5
Saccharomyces cerevisiae (baker's yeast)	YSH1	NP_013379.1
Schizosaccharomyces pombe (fission yeast)	ysh1	NP_594263.2
Arabidopsis thaliana (thale cress)	CPSF73-I	NP_001031215.1
Pan troglodytes (chimpanzee)	CPSF3	NP_001233296.1
	CPSF3	XP_002799184.1
Bos taurus (cattle)	CPSF3	NP_776709.1
Rattus norvegicus (Norway rat)	Cpsf3	NP_001025201.1
Gallus gallus (chicken)	CPSF3	XP_419942.4
Drosophila melanogaster (fruit fly)	Cpsf73	NP_650738.1
Neurospora crassa	NCU03479	XP_956368.1
Xenopus tropicalis (tropical clawed frog)	cpsf3	NP_001006770.1

Gene Ontology
Bibliography

Related articles in PubMed

HPV-16 E2 contributes to induction of HPV-16 late gene expression by inhibiting early polyadenylation.
Johansson C, Somberg M, Li X, Backstr?m Winquist E, Fay J, Ryan F, Pim D, Banks L, Schwartz S
The EMBO journal31(14)3212-27(2012 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I.
Jenny A, Minvielle-Sebastia L, Preker PJ, Keller W
Science (New York, N.Y.)274(5292)1514-7(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CPSF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPSF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43617	NM_174284.2 Latest version!	Bos taurus cleavage and polyadenylation specific factor 3 (CPSF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa91920	XM_015473528.1 Latest version!	Bos taurus cleavage and polyadenylation specific factor 3 (CPSF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CPSF3 ( NM_174284.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776709.1 Bos taurus cleavage and polyadenylation specific factor 3 (CPSF3), mRNA.

Price & Availability↑

CloneID	OBa43617
Clone ID Related Accession (Same CDS sequence)	NM_174284.2
Accession Version	NM_174284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	cleavage and polyadenylation specificity factor subunit 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X95906.1. On Jun 3, 2003 this sequence version replaced NM_174284.1. ##Evidence-Data-START## Transcript exon combination :: X95906.1, BC104553.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGCGA TTCCTGCAGA GGAGAGTGAC CAGCTGCTGA TCCGACCCCT TGGAGCTGGG 
CAGGAAGTGG GAAGATCATG TATTATTCTG GAGTTCAAGG GGAGGAAGAT AATGCTGGAC 
TGTGGGATCC ATCCTGGCCT AGAAGGAATG GATGCTCTTC CTTATATTGA TTTGATTGAC 
CCAGCTGAGA TTGATCTCCT ACTAATTAGT CATTTCCATT TGGATCACTG TGGAGCTTTG 
CCCTGGTTTC TACAGAAGAC AAGTTTCAAA GGAAGAACAT TTATGACTCA TGCTACAAAA 
GCTATTTATA GATGGCTTCT TTCAGATTAT GTCAAAGTTA GTAACATATC AGCAGACGAC 
ATGCTGTATA CTGAGACAGA TTTGGAAGAA AGCATGGACA AAATTGAAAC CATCAATTTT 
CATGAAGTGA AGGAAGTTGC AGGAATCAAG TTTTGGTGTT ATCACGCCGG CCATGTCCTG 
GGAGCTGCCA TGTTCATGAT CGAGATCGCA GGCGTGAAGC TTTTATACAC AGGTGATTTC 
TCAAGACAAG AAGACAGACA CTTAATGGCA GCTGAAATTC CTAACATTAA ACCTGATATT 
CTTATCATTG AATCTACTTA TGGGACCCAT ATCCACGAGA AGCGAGAGGA GCGAGAGGCA 
AGGTTCTGTA ATACCGTCCA TGATATTGTG AACAGAGGGG GCCGAGGCCT CATTCCYGTC 
TTTGCYCTCG GACGGGCCCA GGAACTCCTC TTGATTTTAG ATGAGTACTG GCAGAAYCAC 
CCAGAACTCC ATGATATTCC AATATACTAT GCATCATCTT TGGCCAAGAA GTGTATGGCA 
GTGTACCAGA CATATGTGAA YGCCATGAAT GACAAAATCC GCAAACAGAT CAACATCAAT 
AATCCATTTG TTTTCAAACA CATTAGTAAT CTGAAGAGCA TGGATCATTT TGATGACATT 
GGTCCCAGCG TGGTCATGGC CTCCCCAGGC ATGATGCAAA GTGGTTTATC CAGAGAGCTC 
TTTGAAAGCT GGTGTACTGA TAAGAGGAAT GGCGTCATCA TAGCCGGCTA CTGTGTAGAA 
GGCACGCTTG CCAAGCATAT CATGTCTGAA CCGGAAGAAA TCACTACCAT GTCCGGGCAG 
AAGTTACCTC TGAAAATGTC TGTTGATTAC ATCTCTTTCT CTGCGCACAC GGATTACCAG 
CAAACCAGTG AATTTATACG TGCTTTGAAG CCGCCTCAYG TGATYTTAGT CCATGGAGAA 
CAGAATGAAA TGGCCAGATT GAAAGCAGCC CTGATTCGAG AATATGAAGA TAATGATGAR 
GTTCACATAG AGGTTCATAA TCCTCGGAAT ACAGAGGCAG TGACCTTGAA CTTCAGGGGA 
GAAAAACTAG CCAAGGTCAT GGGATTTTTA GCAGACAAAA AACCAGAACA GGGCCAGCGG 
GTCTCAGGAA TACTTGTTAA AAGAAACTTT AATTATCACA TACTTTCTCC TTGTGACCTC 
TCCAATTATA CTGACTTGGC CATGAGCACT GTAAAACAGA CCCAAGCCAT TCCATATACT 
GGTCCTTTTA ATTTGCTCTA TTACCAGCTA CAGAAATTGA CAGGTGATGT GGAAGAATTA 
GAAATTCAAG AAAAGCCTGC TTTGAAAGTG TTTAAAAATA TTACTGTAAT ACAGGAACCA 
GGAATGGTGG TATTAGAATG GTTGGCAAAT CCTTCCAATG ATATGTATGC AGATACAGTC 
ACAACCGTGA TACTAGAAGT TCAGTCAAAC CCAAAAATAA GGAAAGGTGC AGTACAGAAG 
GTTTCTAAAA AATTAGAAAT GCACGTTTAC AGCAAGAGGC TGGAGATCAT GCTCCAGGAC 
ATATTTGGAG AAGACTGTGT TAGTGTAAAA GATGGTTCTA TTCTTAGTGT CACAGTGGAT 
GGAAAAACTG CTAACATAAA TTTGGAGACA CGGACTGTAG AATGTGAAGA AGGCAGTGAA 
GATGACGAGT CCCTCCGAGA AATGGTGGAA CTGGCGGCCC AGAGACTGTA CGAGGCCCTC 
ACACCCGTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776709.1
CDS	106..2160
Translation	MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLISHFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINFHEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEKREEREARFCNTVHDIVNRGGRGLInullVFnullLGRAQELLLILDEYWQnullHPELHDIPIYYASSLAKKCMAVYQTYVnullAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQSGLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPnullVnullLVHGEQNEMARLKAALIREYEDNDnullVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLYYQLQKLTGDVEELEIQEKPALKVFKNITVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGEDCVSVKDGSILSVTVDGKTANINLETRTVECEEGSEDDESLREMVELAAQRLYEALTPVH

Target ORF information:

RefSeq Version	NM_174284.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cleavage and polyadenylation specific factor 3 (CPSF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174284.2

ORF Insert Sequence:

ATGTCCGCGA TTCCTGCAGA GGAGAGTGAC CAGCTGCTGA TCCGACCCCT TGGAGCTGGG 
CAGGAAGTGG GAAGATCATG TATTATTCTG GAGTTCAAGG GGAGGAAGAT AATGCTGGAC 
TGTGGGATCC ATCCTGGCCT AGAAGGAATG GATGCTCTTC CTTATATTGA TTTGATTGAC 
CCAGCTGAGA TTGATCTCCT ACTAATTAGT CATTTCCATT TGGATCACTG TGGAGCTTTG 
CCCTGGTTTC TACAGAAGAC AAGTTTCAAA GGAAGAACAT TTATGACTCA TGCTACAAAA 
GCTATTTATA GATGGCTTCT TTCAGATTAT GTCAAAGTTA GTAACATATC AGCAGACGAC 
ATGCTGTATA CTGAGACAGA TTTGGAAGAA AGCATGGACA AAATTGAAAC CATCAATTTT 
CATGAAGTGA AGGAAGTTGC AGGAATCAAG TTTTGGTGTT ATCACGCCGG CCATGTCCTG 
GGAGCTGCCA TGTTCATGAT CGAGATCGCA GGCGTGAAGC TTTTATACAC AGGTGATTTC 
TCAAGACAAG AAGACAGACA CTTAATGGCA GCTGAAATTC CTAACATTAA ACCTGATATT 
CTTATCATTG AATCTACTTA TGGGACCCAT ATCCACGAGA AGCGAGAGGA GCGAGAGGCA 
AGGTTCTGTA ATACCGTCCA TGATATTGTG AACAGAGGGG GCCGAGGCCT CATTCCYGTC 
TTTGCYCTCG GACGGGCCCA GGAACTCCTC TTGATTTTAG ATGAGTACTG GCAGAAYCAC 
CCAGAACTCC ATGATATTCC AATATACTAT GCATCATCTT TGGCCAAGAA GTGTATGGCA 
GTGTACCAGA CATATGTGAA YGCCATGAAT GACAAAATCC GCAAACAGAT CAACATCAAT 
AATCCATTTG TTTTCAAACA CATTAGTAAT CTGAAGAGCA TGGATCATTT TGATGACATT 
GGTCCCAGCG TGGTCATGGC CTCCCCAGGC ATGATGCAAA GTGGTTTATC CAGAGAGCTC 
TTTGAAAGCT GGTGTACTGA TAAGAGGAAT GGCGTCATCA TAGCCGGCTA CTGTGTAGAA 
GGCACGCTTG CCAAGCATAT CATGTCTGAA CCGGAAGAAA TCACTACCAT GTCCGGGCAG 
AAGTTACCTC TGAAAATGTC TGTTGATTAC ATCTCTTTCT CTGCGCACAC GGATTACCAG 
CAAACCAGTG AATTTATACG TGCTTTGAAG CCGCCTCAYG TGATYTTAGT CCATGGAGAA 
CAGAATGAAA TGGCCAGATT GAAAGCAGCC CTGATTCGAG AATATGAAGA TAATGATGAR 
GTTCACATAG AGGTTCATAA TCCTCGGAAT ACAGAGGCAG TGACCTTGAA CTTCAGGGGA 
GAAAAACTAG CCAAGGTCAT GGGATTTTTA GCAGACAAAA AACCAGAACA GGGCCAGCGG 
GTCTCAGGAA TACTTGTTAA AAGAAACTTT AATTATCACA TACTTTCTCC TTGTGACCTC 
TCCAATTATA CTGACTTGGC CATGAGCACT GTAAAACAGA CCCAAGCCAT TCCATATACT 
GGTCCTTTTA ATTTGCTCTA TTACCAGCTA CAGAAATTGA CAGGTGATGT GGAAGAATTA 
GAAATTCAAG AAAAGCCTGC TTTGAAAGTG TTTAAAAATA TTACTGTAAT ACAGGAACCA 
GGAATGGTGG TATTAGAATG GTTGGCAAAT CCTTCCAATG ATATGTATGC AGATACAGTC 
ACAACCGTGA TACTAGAAGT TCAGTCAAAC CCAAAAATAA GGAAAGGTGC AGTACAGAAG 
GTTTCTAAAA AATTAGAAAT GCACGTTTAC AGCAAGAGGC TGGAGATCAT GCTCCAGGAC 
ATATTTGGAG AAGACTGTGT TAGTGTAAAA GATGGTTCTA TTCTTAGTGT CACAGTGGAT 
GGAAAAACTG CTAACATAAA TTTGGAGACA CGGACTGTAG AATGTGAAGA AGGCAGTGAA 
GATGACGAGT CCCTCCGAGA AATGGTGGAA CTGGCGGCCC AGAGACTGTA CGAGGCCCTC 
ACACCCGTCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPSF3 ( XM_015473528.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329014.1 Bos taurus cleavage and polyadenylation specific factor 3 (CPSF3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa91920
Clone ID Related Accession (Same CDS sequence)	XM_015473528.1
Accession Version	XM_015473528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cleavage and polyadenylation specificity factor subunit 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCATG CTACAAAAGC TATTTATAGA TGGCTTCTTT CAGATTATGT CAAAGTTAGT 
AACATATCAG CAGACGACAT GCTGTATACT GAGACAGATT TGGAAGAAAG CATGGACAAA 
ATTGAAACCA TCAATTTTCA TGAAGTGAAG GAAGTTGCAG GAATCAAGTT TTGGTGTTAT 
CACGCCGGCC ATGTCCTGGG AGCTGCCATG TTCATGATCG AGATCGCAGG CGTGAAGCTT 
TTATACACAG GTGATTTCTC AAGACAAGAA GACAGACACT TAATGGCAGC TGAAATTCCT 
AACATTAAAC CTGATATTCT TATCATTGAA TCTACTTATG GGACCCATAT CCACGAGAAG 
CGAGAGGAGC GAGAGGCAAG GTTCTGTAAT ACCGTCCATG ATATTGTGAA CAGAGGGGGC 
CGAGGCCTCA TTCCTGTCTT TGCCCTCGGA CGGGCCCAGG AACTCCTCTT GATTTTAGAT 
GAGTACTGGC AGAATCACCC AGAACTCCAT GATATTCCAA TATACTATGC ATCATCTTTG 
GCCAAGAAGT GTATGGCAGT GTACCAGACA TATGTGAACG CCATGAATGA CAAAATCCGC 
AAACAGATCA ACATCAATAA TCCGTTTGTT TTCAAACACA TTAGTAATCT GAAGAGCATG 
GATCATTTTG ATGACATTGG TCCCAGCGTG GTCATGGCCT CCCCAGGCAT GATGCAAAGT 
GGTTTATCCA GAGAGCTCTT TGAAAGCTGG TGTACTGATA AGAGGAATGG CGTCATCATA 
GCCGGCTACT GTGTAGAAGG CACGCTTGCC AAGCATATCA TGTCTGAACC GGAAGAAATC 
ACTACCATGT CCGGGCAGAA GTTACCTCTG AAAATGTCTG TTGATTACAT CTCTTTCTCT 
GCGCACACGG ATTACCAGCA AACCAGTGAA TTTATACGTG CTTTGAAGCC GCCTCATGTG 
ATCTTAGTCC ATGGAGAACA GAATGAAATG GCCAGATTGA AAGCAGCCCT GATTCGAGAA 
TATGAAGATA ATGATGAAGT TCACATAGAG GTTCATAATC CTCGGAATAC AGAGGCAGTG 
ACCTTGAACT TCAGGGGAGA AAAACTAGCC AAGGTCATGG GATTTTTAGC AGACAAAAAA 
CCAGAACAGG GCCAGCGGGT CTCAGGAATA CTTGTTAAAA GAAACTTTAA TTATCACATA 
CTTTCTCCTT GTGACCTCTC CAATTATACT GACTTGGCCA TGAGCACTGT AAAACAGACC 
CAAGCCATTC CATATACTGG TCCTTTTAAT TTGCTCTATT ACCAGCTACA GAAATTGACA 
GGTGATGTGG AAGAATTAGA AATTCAAGAA AAGCCTGCTT TGAAAGTGTT TAAAAATATT 
ACTGTAATAC AGGAACCAGG AATGGTGGTA TTAGAATGGT TGGCAAATCC TTCCAATGAT 
ATGTATGCAG ATACAGTCAC AACCGTGATA CTAGAAGTTC AGTCAAACCC AAAAATAAGG 
AAAGGTGCAG TACAGAAGGT TTCTAAAAAA TTAGAAATGC ACGTTTACAG CAAGAGGCTG 
GAGATCATGC TCCAGGACAT ATTTGGAGAA GACTGTGTTA GTGTAAAAGA TGGTTCTATT 
CTTAGTGTCA CAGTGGATGG AAAAACTGCT AACATAAATT TGGAGACACG GACTGTAGAA 
TGTGAAGAAG GCAGTGAAGA TGACGAGTCC CTCCGAGAAA TGGTGGAACT GGCGGCCCAG 
AGACTGTACG AGGCCCTCAC ACCCGTCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329014.1
CDS	166..1938
Translation	MTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINFHEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTHIHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMAVYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQSGLSRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLYYQLQKLTGDVEELEIQEKPALKVFKNITVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGEDCVSVKDGSILSVTVDGKTANINLETRTVECEEGSEDDESLREMVELAAQRLYEALTPVH

Target ORF information:

RefSeq Version	XM_015473528.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cleavage and polyadenylation specific factor 3 (CPSF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473528.1

ORF Insert Sequence:

ATGACTCATG CTACAAAAGC TATTTATAGA TGGCTTCTTT CAGATTATGT CAAAGTTAGT 
AACATATCAG CAGACGACAT GCTGTATACT GAGACAGATT TGGAAGAAAG CATGGACAAA 
ATTGAAACCA TCAATTTTCA TGAAGTGAAG GAAGTTGCAG GAATCAAGTT TTGGTGTTAT 
CACGCCGGCC ATGTCCTGGG AGCTGCCATG TTCATGATCG AGATCGCAGG CGTGAAGCTT 
TTATACACAG GTGATTTCTC AAGACAAGAA GACAGACACT TAATGGCAGC TGAAATTCCT 
AACATTAAAC CTGATATTCT TATCATTGAA TCTACTTATG GGACCCATAT CCACGAGAAG 
CGAGAGGAGC GAGAGGCAAG GTTCTGTAAT ACCGTCCATG ATATTGTGAA CAGAGGGGGC 
CGAGGCCTCA TTCCTGTCTT TGCCCTCGGA CGGGCCCAGG AACTCCTCTT GATTTTAGAT 
GAGTACTGGC AGAATCACCC AGAACTCCAT GATATTCCAA TATACTATGC ATCATCTTTG 
GCCAAGAAGT GTATGGCAGT GTACCAGACA TATGTGAACG CCATGAATGA CAAAATCCGC 
AAACAGATCA ACATCAATAA TCCGTTTGTT TTCAAACACA TTAGTAATCT GAAGAGCATG 
GATCATTTTG ATGACATTGG TCCCAGCGTG GTCATGGCCT CCCCAGGCAT GATGCAAAGT 
GGTTTATCCA GAGAGCTCTT TGAAAGCTGG TGTACTGATA AGAGGAATGG CGTCATCATA 
GCCGGCTACT GTGTAGAAGG CACGCTTGCC AAGCATATCA TGTCTGAACC GGAAGAAATC 
ACTACCATGT CCGGGCAGAA GTTACCTCTG AAAATGTCTG TTGATTACAT CTCTTTCTCT 
GCGCACACGG ATTACCAGCA AACCAGTGAA TTTATACGTG CTTTGAAGCC GCCTCATGTG 
ATCTTAGTCC ATGGAGAACA GAATGAAATG GCCAGATTGA AAGCAGCCCT GATTCGAGAA 
TATGAAGATA ATGATGAAGT TCACATAGAG GTTCATAATC CTCGGAATAC AGAGGCAGTG 
ACCTTGAACT TCAGGGGAGA AAAACTAGCC AAGGTCATGG GATTTTTAGC AGACAAAAAA 
CCAGAACAGG GCCAGCGGGT CTCAGGAATA CTTGTTAAAA GAAACTTTAA TTATCACATA 
CTTTCTCCTT GTGACCTCTC CAATTATACT GACTTGGCCA TGAGCACTGT AAAACAGACC 
CAAGCCATTC CATATACTGG TCCTTTTAAT TTGCTCTATT ACCAGCTACA GAAATTGACA 
GGTGATGTGG AAGAATTAGA AATTCAAGAA AAGCCTGCTT TGAAAGTGTT TAAAAATATT 
ACTGTAATAC AGGAACCAGG AATGGTGGTA TTAGAATGGT TGGCAAATCC TTCCAATGAT 
ATGTATGCAG ATACAGTCAC AACCGTGATA CTAGAAGTTC AGTCAAACCC AAAAATAAGG 
AAAGGTGCAG TACAGAAGGT TTCTAAAAAA TTAGAAATGC ACGTTTACAG CAAGAGGCTG 
GAGATCATGC TCCAGGACAT ATTTGGAGAA GACTGTGTTA GTGTAAAAGA TGGTTCTATT 
CTTAGTGTCA CAGTGGATGG AAAAACTGCT AACATAAATT TGGAGACACG GACTGTAGAA 
TGTGAAGAAG GCAGTGAAGA TGACGAGTCC CTCCGAGAAA TGGTGGAACT GGCGGCCCAG 
AGACTGTACG AGGCCCTCAC ACCCGTCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HPV-16 E2 contributes to induction of HPV-16 late gene expression by inhibiting early polyadenylation.
The EMBO journal31(14)3212-27(2012 May)
Johansson C,Somberg M,Li X,Backstr?m Winquist E,Fay J,Ryan F,Pim D,Banks L,Schwartz S

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I.
Science (New York, N.Y.)274(5292)1514-7(1996 Nov)
Jenny A,Minvielle-Sebastia L,Preker PJ,Keller W

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CPSF3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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