CNGA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNGA1
Entrez Gene ID	281700
Full Name	cyclic nucleotide gated channel alpha 1
Synonyms	CNCG1,CNG
General protein information	Preferred Names cyclic nucleotide gated channel alpha 1 Names cGMP-gated cation channel alpha-1 CNG channel alpha-1 CNG-1 CNG1 cyclic nucleotide gated chanel (photoreceptor), cGMP gated 1, alpha cyclic nucleotide gated channel (photoreceptor), cGMP gated cyclic nucleotide-gated cation channel 1 cyclic nucleotide-gated channel, photoreceptor rod photoreceptor cGMP-gated channel subunit alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174278.2	NP_776703.1	cGMP-gated cation channel alpha-1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cnga1	NP_031749.2
Gallus gallus (chicken)	CNGA1	NP_990551.1
Pan troglodytes (chimpanzee)	CNGA1	XP_001152062.2
Danio rerio (zebrafish)	cnga1	XP_701036.4
Bos taurus (cattle)	CNGA1	NP_776703.1
Xenopus tropicalis (tropical clawed frog)	cnga1	XP_002933507.1
Homo sapiens (human)	CNGA1	NP_001136036.1
	CNGA1	XP_002804108.1
Canis lupus familiaris (dog)	CNGA1	NP_001003222.1
Rattus norvegicus (Norway rat)	Cnga1	NP_445949.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of CNGA1 Channel Gating by Interactions with the Membrane.
Aman TK, Gordon SE, Zagotta WN
The Journal of biological chemistry291(19)9939-47(2016 May)

A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels.
Napolitano LM, Bisha I, De March M, Marchesi A, Arcangeletti M, Demitri N, Mazzolini M, Rodriguez A, Magistrato A, Onesti S, Laio A, Torre V
Proceedings of the National Academy of Sciences of the United States of America112(27)E3619-28(2015 Jul)

Conformational rearrangements in the transmembrane domain of CNGA1 channels revealed by single-molecule force spectroscopy.
Maity S, Mazzolini M, Arcangeletti M, Valbuena A, Fabris P, Lazzarino M, Torre V
Nature communications67093(2015 May)

Cyclic nucleotide-gated channel subunit glycosylation regulates matrix metalloproteinase-dependent changes in channel gating.
Meighan SE, Meighan PC, Rich ED, Brown RL, Varnum MD
Biochemistry52(46)8352-62(2013 Nov)

A secondary structural transition in the C-helix promotes gating of cyclic nucleotide-regulated ion channels.
Puljung MC, Zagotta WN
The Journal of biological chemistry288(18)12944-56(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that restraining the A' helix to the plasma membrane potentiates CNGA1 channel opening.
Title: Regulation of CNGA1 Channel Gating by Interactions with the Membrane.

Data show that when Pro365 is replaced with a threonine in cyclic nucleotide gated channel alpha 1 (CNGA1), cesium Cs+ permeation is nearly abolished.
Title: A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels.

Conformation changes of CNGA1 associated with its gating function are reported.
Title: Conformational rearrangements in the transmembrane domain of CNGA1 channels revealed by single-molecule force spectroscopy.

Stabilizing the C-helix of intact CNGA1 channels by metal binding to a pair of histidines promoted channel opening.
Title: A secondary structural transition in the C-helix promotes gating of cyclic nucleotide-regulated ion channels.

A ring of threonines in the inner vestibule of the pore of CNGA1 channels constitutes a binding site for permeating ions
Title: A ring of threonines in the inner vestibule of the pore of CNGA1 channels constitutes a binding site for permeating ions.

The following CNGA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNGA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43711	NM_174278.2 Latest version!	Bos taurus cyclic nucleotide gated channel subunit alpha 1 (CNGA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNGA1 ( NM_174278.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776703.1 Bos taurus cyclic nucleotide gated channel subunit alpha 1 (CNGA1), mRNA.

Price & Availability↑

CloneID	OBa43711
Clone ID Related Accession (Same CDS sequence)	NM_174278.2
Accession Version	NM_174278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	cGMP-gated cation channel alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X51604.1. On Jun 3, 2003 this sequence version replaced NM_174278.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X51604.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145415, SAMN03145416 [ECO:0000350] ##Evidence-Data-END##

ATGAAGAAAG TGATTATCAA TACATGGCAC TCTTTTGTAA ATATTCCCAA TGTGGTTGGA 
CCAGATGTTG AAAAGGAAAT AACAAGGATG GAAAATGGAG CATGCAGCTC CTTTTCTGGT 
GATGATGACG ACAGTGCCTC TATGTTTGAA GAATCAGAGA CTGAGAACCC CCACGCAAGG 
GATTCCTTTA GAAGTAATAC ACACGGAAGC GGACAACCAT CACAGAGGGA GCAATACCTG 
CCTGGAGCCA TTGCACTTTT TAATGTTAAC AACAGCAGCA ATAAGGAGCA AGAACCAAAG 
GAAAAGAAGA AAAAGAAAAA AGAAAAGAAG AGCAAGCCAG ATGATAAAAA TGAAAATAAA 
AAGGACCCAG AAAAGAAAAA GAAGAAAGAA AAGGACAAAG ACAAGAAAAA GAAAGAGGAG 
AAAGGCAAAG ATAAGAAAGA GGAAGAGAAG AAGGAAGTCG TGGTTATTGA TCCCTCAGGA 
AATACGTATT ACAACTGGCT GTTCTGCATC ACCTTACCTG TTATGTATAA CTGGACCATG 
ATTATTGCAA GAGCATGTTT TGATGAACTT CAGTCTGATT ACCTAGAATA CTGGCTTGCT 
TTTGATTACT TATCAGATGT AGTCTATCTT CTTGATATGT TTGTACGAAC AAGGACAGGT 
TACCTAGAAC AAGGACTACT GGTGAAGGAA GAGCGTAAAC TCATAGACAA GTATAAATCA 
ACCTTTCAAT TTAAACTTGA TGTTCTATCA GTGATCCCAA CTGATCTGCT GTATATTAAG 
TTTGGCTGGA ATTATCCAGA AATTAGATTA AACAGATTGT TAAGGATCTC TCGAATGTTT 
GAGTTCTTCC AGAGAACAGA AACACGGACA AACTACCCAA ACATCTTCAG GATCTCTAAC 
CTTGTCATGT ACATCATCAT CATCATCCAC TGGAATGCGT GTGTGTACTT CTCTATTTCC 
AAAGCTATTG GGTTTGGAAA TGACACATGG GTCTATCCTG ATGTTAATGA TCCTGATTTT 
GGCCGTTTGG CTAGAAAATA TGTGTACAGT CTTTACTGGT CTACACTGAC TTTGACCACT 
ATTGGCGAAA CACCACCTCC TGTGAGGGAT TCTGAGTATT TCTTTGTGGT GGCTGATTTC 
CTCATTGGAG TGTTAATTTT TGCCACCATT GTCGGTAACA TAGGTTCTAT GATTTCCAAC 
ATGAATGCGG CCAGGGCAGA ATTTCAAGCA AGAATTGATG CAATCAAGCA ATACATGCAT 
TTTCGAAATG TAAGCAAAGA TATGGAGAAG CGAGTCATTA AATGGTTTGA CTATCTGTGG 
ACCAACAAAA AAACAGTGGA TGAGAGAGAA GTCTTGAAGT ATCTACCTGA TAAACTAAGA 
GCAGAGATCG CCATCAATGT TCATTTAGAC ACATTAAAAA AGGTGCGCAT TTTTGCAGAC 
TGTGAAGCTG GTCTGTTGGT GGAGTTGGTC TTGAAATTAC AACCCCAAGT CTACAGTCCT 
GGAGATTACA TTTGCAAGAA AGGGGACATT GGCCGAGAGA TGTACATCAT CAAGGAAGGC 
AAACTCGCCG TGGTGGCTGA TGACGGGATC ACTCAGTTTG TAGTATTGAG TGATGGCAGC 
TACTTTGGTG AAATCAGTAT CCTTAATATT AAAGGTAGCA AAGCTGGCAA TCGAAGAACA 
GCCAATATTA AAAGCATTGG CTACTCAGAT CTATTCTGTC TCTCAAAAGA TGACCTCATG 
GAAGCTCTAA CTGAGTACCC AGATGCCAAA GGTATGCTAG AAGAGAAAGG GAAGCAAATT 
TTGATGAAAG ATGGTCTATT GGACATAAAC ATTGCAAATG CTGGAAGTGA TCCTAAAGAT 
CTTGAAGAGA AGGTCACCCG AATGGAGAGC TCAGTAGACC TCCTGCAAAC AAGGTTTGCT 
CGGATCCTGG CTGAGTATGA GTCAATGCAG CAGAAACTGA AGCAGAGGCT AACCAAGGTT 
GAGAAATTCC TGAAACCACT TATTGACACA GAATTTTCAG CTATTGAAGG ATCTGGAACT 
GAAAGTGGGC CCACAGACTC TACACAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776703.1
CDS	195..2267
Translation	MKKVIINTWHSFVNIPNVVGPDVEKEITRMENGACSSFSGDDDDSASMFEESETENPHARDSFRSNTHGSGQPSQREQYLPGAIALFNVNNSSNKEQEPKEKKKKKKEKKSKPDDKNENKKDPEKKKKKEKDKDKKKKEEKGKDKKEEEKKEVVVIDPSGNTYYNWLFCITLPVMYNWTMIIARACFDELQSDYLEYWLAFDYLSDVVYLLDMFVRTRTGYLEQGLLVKEERKLIDKYKSTFQFKLDVLSVIPTDLLYIKFGWNYPEIRLNRLLRISRMFEFFQRTETRTNYPNIFRISNLVMYIIIIIHWNACVYFSISKAIGFGNDTWVYPDVNDPDFGRLARKYVYSLYWSTLTLTTIGETPPPVRDSEYFFVVADFLIGVLIFATIVGNIGSMISNMNAARAEFQARIDAIKQYMHFRNVSKDMEKRVIKWFDYLWTNKKTVDEREVLKYLPDKLRAEIAINVHLDTLKKVRIFADCEAGLLVELVLKLQPQVYSPGDYICKKGDIGREMYIIKEGKLAVVADDGITQFVVLSDGSYFGEISILNIKGSKAGNRRTANIKSIGYSDLFCLSKDDLMEALTEYPDAKGMLEEKGKQILMKDGLLDINIANAGSDPKDLEEKVTRMESSVDLLQTRFARILAEYESMQQKLKQRLTKVEKFLKPLIDTEFSAIEGSGTESGPTDSTQD

Target ORF information:

RefSeq Version	NM_174278.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cyclic nucleotide gated channel subunit alpha 1 (CNGA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174278.2

ORF Insert Sequence:

ATGAAGAAAG TGATTATCAA TACATGGCAC TCTTTTGTAA ATATTCCCAA TGTGGTTGGA 
CCAGATGTTG AAAAGGAAAT AACAAGGATG GAAAATGGAG CATGCAGCTC CTTTTCTGGT 
GATGATGACG ACAGTGCCTC TATGTTTGAA GAATCAGAGA CTGAGAACCC CCACGCAAGG 
GATTCCTTTA GAAGTAATAC ACACGGAAGC GGACAACCAT CACAGAGGGA GCAATACCTG 
CCTGGAGCCA TTGCACTTTT TAATGTTAAC AACAGCAGCA ATAAGGAGCA AGAACCAAAG 
GAAAAGAAGA AAAAGAAAAA AGAAAAGAAG AGCAAGCCAG ATGATAAAAA TGAAAATAAA 
AAGGACCCAG AAAAGAAAAA GAAGAAAGAA AAGGACAAAG ACAAGAAAAA GAAAGAGGAG 
AAAGGCAAAG ATAAGAAAGA GGAAGAGAAG AAGGAAGTCG TGGTTATTGA TCCCTCAGGA 
AATACGTATT ACAACTGGCT GTTCTGCATC ACCTTACCTG TTATGTATAA CTGGACCATG 
ATTATTGCAA GAGCATGTTT TGATGAACTT CAGTCTGATT ACCTAGAATA CTGGCTTGCT 
TTTGATTACT TATCAGATGT AGTCTATCTT CTTGATATGT TTGTACGAAC AAGGACAGGT 
TACCTAGAAC AAGGACTACT GGTGAAGGAA GAGCGTAAAC TCATAGACAA GTATAAATCA 
ACCTTTCAAT TTAAACTTGA TGTTCTATCA GTGATCCCAA CTGATCTGCT GTATATTAAG 
TTTGGCTGGA ATTATCCAGA AATTAGATTA AACAGATTGT TAAGGATCTC TCGAATGTTT 
GAGTTCTTCC AGAGAACAGA AACACGGACA AACTACCCAA ACATCTTCAG GATCTCTAAC 
CTTGTCATGT ACATCATCAT CATCATCCAC TGGAATGCGT GTGTGTACTT CTCTATTTCC 
AAAGCTATTG GGTTTGGAAA TGACACATGG GTCTATCCTG ATGTTAATGA TCCTGATTTT 
GGCCGTTTGG CTAGAAAATA TGTGTACAGT CTTTACTGGT CTACACTGAC TTTGACCACT 
ATTGGCGAAA CACCACCTCC TGTGAGGGAT TCTGAGTATT TCTTTGTGGT GGCTGATTTC 
CTCATTGGAG TGTTAATTTT TGCCACCATT GTCGGTAACA TAGGTTCTAT GATTTCCAAC 
ATGAATGCGG CCAGGGCAGA ATTTCAAGCA AGAATTGATG CAATCAAGCA ATACATGCAT 
TTTCGAAATG TAAGCAAAGA TATGGAGAAG CGAGTCATTA AATGGTTTGA CTATCTGTGG 
ACCAACAAAA AAACAGTGGA TGAGAGAGAA GTCTTGAAGT ATCTACCTGA TAAACTAAGA 
GCAGAGATCG CCATCAATGT TCATTTAGAC ACATTAAAAA AGGTGCGCAT TTTTGCAGAC 
TGTGAAGCTG GTCTGTTGGT GGAGTTGGTC TTGAAATTAC AACCCCAAGT CTACAGTCCT 
GGAGATTACA TTTGCAAGAA AGGGGACATT GGCCGAGAGA TGTACATCAT CAAGGAAGGC 
AAACTCGCCG TGGTGGCTGA TGACGGGATC ACTCAGTTTG TAGTATTGAG TGATGGCAGC 
TACTTTGGTG AAATCAGTAT CCTTAATATT AAAGGTAGCA AAGCTGGCAA TCGAAGAACA 
GCCAATATTA AAAGCATTGG CTACTCAGAT CTATTCTGTC TCTCAAAAGA TGACCTCATG 
GAAGCTCTAA CTGAGTACCC AGATGCCAAA GGTATGCTAG AAGAGAAAGG GAAGCAAATT 
TTGATGAAAG ATGGTCTATT GGACATAAAC ATTGCAAATG CTGGAAGTGA TCCTAAAGAT 
CTTGAAGAGA AGGTCACCCG AATGGAGAGC TCAGTAGACC TCCTGCAAAC AAGGTTTGCT 
CGGATCCTGG CTGAGTATGA GTCAATGCAG CAGAAACTGA AGCAGAGGCT AACCAAGGTT 
GAGAAATTCC TGAAACCACT TATTGACACA GAATTTTCAG CTATTGAAGG ATCTGGAACT 
GAAAGTGGGC CCACAGACTC TACACAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of CNGA1 Channel Gating by Interactions with the Membrane.
The Journal of biological chemistry291(19)9939-47(2016 May)
Aman TK,Gordon SE,Zagotta WN

A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels.
Proceedings of the National Academy of Sciences of the United States of America112(27)E3619-28(2015 Jul)
Napolitano LM,Bisha I,De March M,Marchesi A,Arcangeletti M,Demitri N,Mazzolini M,Rodriguez A,Magistrato A,Onesti S,Laio A,Torre V

Conformational rearrangements in the transmembrane domain of CNGA1 channels revealed by single-molecule force spectroscopy.
Nature communications67093(2015 May)
Maity S,Mazzolini M,Arcangeletti M,Valbuena A,Fabris P,Lazzarino M,Torre V

Cyclic nucleotide-gated channel subunit glycosylation regulates matrix metalloproteinase-dependent changes in channel gating.
Biochemistry52(46)8352-62(2013 Nov)
Meighan SE,Meighan PC,Rich ED,Brown RL,Varnum MD

A secondary structural transition in the C-helix promotes gating of cyclic nucleotide-regulated ion channels.
The Journal of biological chemistry288(18)12944-56(2013 May)
Puljung MC,Zagotta WN

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CNGA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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