CAPN1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN1
Entrez Gene ID	281661
Full Name	calpain 1
Synonyms	CANP 1,CANP1
General protein information	Preferred Names calpain 1 Names calpain-1 catalytic subunit calcium-activated neutral proteinase 1 calpain 1, (mu/I) large subunit calpain mu-type calpain-1 large subunit micromolar calcium-activated neutral protease 1 micromolar-calpain mu-calpain muCANP
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174259.2	NP_776684.1	calpain-1 catalytic subunit
XM_024986972.1	XP_024842740.1	calpain-1 catalytic subunit isoform X1
XM_024986973.1	XP_024842741.1	calpain-1 catalytic subunit isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAPN1	XP_001169100.1
Mus musculus (house mouse)	Capn1	NP_001103974.1
Bos taurus (cattle)	CAPN1	NP_776684.1
Danio rerio (zebrafish)	si:dkeyp-50d11.2	XP_689397.5
Xenopus tropicalis (tropical clawed frog)	capn1	NP_001013632.1
Gallus gallus (chicken)	CAPN1	NP_001038137.1
Danio rerio (zebrafish)	zgc:55262	NP_998624.1
Homo sapiens (human)	CAPN1	NP_001185797.1
	CAPN1	XP_001114172.1
Canis lupus familiaris (dog)	CAPN1	XP_540866.2
Rattus norvegicus (Norway rat)	Capn1	NP_062025.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of single nucleotide polymorphisms in CAPN1, CAST and MB genes with meat color of Brahman and crossbreed cattle.
Castro S, R?os M, Ortiz Y, Manrique C, Jim?nez A, Ariza F
Meat science11744-9(2016 Jul)

CAPN1, CAST, and DGAT1 genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in a beef cattle population selected for haplotype and allele equalization.
Tait RG Jr, Shackelford SD, Wheeler TL, King DA, Keele JW, Casas E, Smith TP, Bennett GL
Journal of animal science92(12)5382-93(2014 Dec)

Understanding the interaction determinants of CAPN1 inhibition by CAST4 from bovines using molecular modeling techniques.
Chai HH, Lim D, Jung E, Choi BH, Cho YM
Molecules (Basel, Switzerland)19(9)14316-51(2014 Sep)

Bovine mu-calpain (CAPN1) gene: new SNP within intron 14.
Juszczuk-Kubiak E, Sakowski T, Flisikowski K, Wicinska K, Oprzadek J, Rosochacki SJ
Journal of applied genetics45(4)457-60(2004)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study is the first report on genetic variant g.-1256 A>C in the promoter region of CAPN1 gene association with the semen quality of Chinese Holstein bulls by influencing its expression
Title: A g.-1256 A>C in the promoter region of CAPN1 is associated with semen quality traits in Chinese Holstein bulls.

These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions.
Title: Association of single nucleotide polymorphisms in CAPN1, CAST and MB genes with meat color of Brahman and crossbreed cattle.

This work is supportive of the importance of CAPN1 and CAST for mean tenderness in beef.
Title: CAPN1, CAST, and DGAT1 genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in a beef cattle population selected for haplotype and allele equalization.

bovine CAPN-CAST interaction were well reflected in the geometry between the pharmacophore features and its shape constraints identified using our modeled bovine CAPN1/CAST4 complex structures
Title: Understanding the interaction determinants of CAPN1 inhibition by CAST4 from bovines using molecular modeling techniques.

This work confirms the importance of CAPN1 and CAST for tenderness in beef, provides a new effect of CAST on beef tenderness, and questions the utility of GHR as a selection marker for beef quality.
Title: ?-Calpain, calpastatin, and growth hormone receptor genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in Angus cattle selected to increase minor haplotype and allele frequencies.

The following CAPN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41262	NM_174259.2 Latest version!	Bos taurus calpain 1 (CAPN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa231831	XM_024986972.1 Latest version!	Bos taurus calpain 1 (CAPN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa231831	XM_024986973.1 Latest version!	Bos taurus calpain 1 (CAPN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN1 ( NM_174259.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776684.1 Bos taurus calpain 1 (CAPN1), mRNA.

Price & Availability↑

CloneID	OBa41262
Clone ID Related Accession (Same CDS sequence)	NM_174259.2
Accession Version	NM_174259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	calpain-1 catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF221129.1. On or before Mar 29, 2005 this sequence version replaced XM_615535.1, NM_174259.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF221129.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGAGG AGTTCATCAC TCCGGTGTAC TGCACCGGGG TGTCTGCACA AGTGCAGAAG 
CAGCGGGCCA AGGAGCTGGG CCTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTACGAGC AGCTGCGGGT TCACTGCCTG CAAAGAGGGG CCCTTTTCCG TGACGAGGCT 
TTCCCCCCAG TGCCCCAGAG CCTGGGCTTC AAGGAGCTGG GCCCCAACTC CTCCAAAACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAG CTGTTCTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GCACGGACAT CTGCCAGGGC GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATCGCCTCCC TTACCCTCAA TGACACCCTC CTGCACCGAG TAGTTCCACA TGGCCAAAGC 
TTCCAGGATG GCTACGCTGG CATCTTCCAT TTCCAGCTGT GGCAGTTTGG TGAGTGGGTG 
GATGTGGTGG TGGATGACCT GCTGCCCACC AAGGACGGGA AGCTGGTGTT TGTGCACTCT 
GCCCAAGGCA ACGAGTTCTG GAGCGCCCTG CTGGAGAAAG CCTATGCCAA GGTGAACGGC 
AGCTACGAGG CCCTCTCAGG AGGCAGCACA TCTGAGGGCT TTGAGGACTT CACCGGCGGA 
GTCACCGAGT GGTACGAGCT GCGCAAGGCG CCCAGCGACC TCTACAACAT CATCCTCAAG 
GCCCTGGAGC GTGGCTCCCT GCTGGGCTGC TCCATCGATA TCTCCAGCAT TCTGGACATG 
GAGGCTGTCA CCTTCAAGAA GCTGGTGAAG GGCCACGCCT ACTCTGTGAC CGGGGCCAAA 
CAGGTGAACT ACCAGGGCCA GATGGTGAAC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CAGGAGCCTG GAGTGACGGC TCCTCGGAGT GGAACGGCGT GGACCCTTAC 
ATGCGGGAGC AGCTCCGGGT CAAGATGGAG GATGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGTG AATTCACCCG CCTGGAGATC TGCAACCTGA CACCCGACGC GCTCAAGAGC 
CAGAGGTTCC GCAACTGGAA CACCACCCTG TATGAGGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGCAACTA CCCAGCCACT TTCTGGGTGA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGA CGGATGACCC GGACCCCGAC GACTACGGGG GTCGCGAGTC AGGCTGCAGC 
TTCCTGCTCG CCCTCATGCA GAAGCACCGC CGTCGGGAGC GCCGTTTCGG CCGTGACATG 
GAGACCATAG GCTTCGCTGT CTACGAGGTC CCTCCGGAGC TGATGGGCCA GCCGGCCGTG 
CATCTGAAGC GGGACTTCTT CCTGTCCAAC GCCTCCCGGG CCCGGTCCGA GCAGTTCATC 
AACCTGCGGG AGGTCAGCAC CCGCTTCCGC CTGCCGCCTG GGGAGTACGT GGTGGTGCCC 
TCCACCTTCG AGCCCAACAA GGAAGGTGAC TTTGTGCTGC GTTTCTTCTC AGAGAAGAGC 
GCAGGGACCC AAGAGCTGGA TGACCAGGTC CAGGCCAATC TCCCCGACGA GCAAGTGCTC 
TCAGAAGAGG AGATTGATGA GAACTTCAAG TCCCTCTTCA GACAACTGGC AGGGGAGGAC 
ATGGAGATCA GCGTCAAGGA GCTGCGGACC ATCCTCAACA GGATCATCAG CAAACACAAA 
GACCTGCGGA CCACGGGCTT CAGCCTGGAG TCCTGCCGCA GCATGGTCAA CCTCATGGAT 
CGCGACGGCA ATGGCAAACT GGGCCTGGTG GAGTTCAACA TCCTATGGAA CCGGATCCGG 
AATTACCTGT CCATCTTCCG GAAGTTTGAC CTGGACAAGT CGGGCAGCAT GAGTGCCTAT 
GAAATGCGGA TGGCCATTGA GTTTGCAGGC TTCAAGCTCA ACAAGAAGCT GTACGAGCTC 
ATTATCACCC GCTACTCGGA GCCCGACCTG GCCGTGGACT TCGACAACTT CGTGTGCTGC 
CTGGTGCGGC TGGAGACCAT GTTCCGGTTT TTCAAAACTC TGGACACTGA TCTGGATGGA 
GTGGTGACCT TTGACTTGTT TAAGTGGTTA CAGCTGACCA TGTTTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776684.1
CDS	78..2228
Translation	MAEEFITPVYCTGVSAQVQKQRAKELGLGRHENAIKYLGQDYEQLRVHCLQRGALFRDEAFPPVPQSLGFKELGPNSSKTYGIKWKRPTELFSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQDGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYNIILKALERGSLLGCSIDISSILDMEAVTFKKLVKGHAYSVTGAKQVNYQGQMVNLIRMRNPWGEVEWTGAWSDGSSEWNGVDPYMREQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSQRFRNWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEETDDPDPDDYGGRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYEVPPELMGQPAVHLKRDFFLSNASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTQELDDQVQANLPDEQVLSEEEIDENFKSLFRQLAGEDMEISVKELRTILNRIISKHKDLRTTGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIEFAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	NM_174259.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 1 (CAPN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174259.2

ORF Insert Sequence:

ATGGCCGAGG AGTTCATCAC TCCGGTGTAC TGCACCGGGG TGTCTGCACA AGTGCAGAAG 
CAGCGGGCCA AGGAGCTGGG CCTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTACGAGC AGCTGCGGGT TCACTGCCTG CAAAGAGGGG CCCTTTTCCG TGACGAGGCT 
TTCCCCCCAG TGCCCCAGAG CCTGGGCTTC AAGGAGCTGG GCCCCAACTC CTCCAAAACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAG CTGTTCTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GCACGGACAT CTGCCAGGGC GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATCGCCTCCC TTACCCTCAA TGACACCCTC CTGCACCGAG TAGTTCCACA TGGCCAAAGC 
TTCCAGGATG GCTACGCTGG CATCTTCCAT TTCCAGCTGT GGCAGTTTGG TGAGTGGGTG 
GATGTGGTGG TGGATGACCT GCTGCCCACC AAGGACGGGA AGCTGGTGTT TGTGCACTCT 
GCCCAAGGCA ACGAGTTCTG GAGCGCCCTG CTGGAGAAAG CCTATGCCAA GGTGAACGGC 
AGCTACGAGG CCCTCTCAGG AGGCAGCACA TCTGAGGGCT TTGAGGACTT CACCGGCGGA 
GTCACCGAGT GGTACGAGCT GCGCAAGGCG CCCAGCGACC TCTACAACAT CATCCTCAAG 
GCCCTGGAGC GTGGCTCCCT GCTGGGCTGC TCCATCGATA TCTCCAGCAT TCTGGACATG 
GAGGCTGTCA CCTTCAAGAA GCTGGTGAAG GGCCACGCCT ACTCTGTGAC CGGGGCCAAA 
CAGGTGAACT ACCAGGGCCA GATGGTGAAC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CAGGAGCCTG GAGTGACGGC TCCTCGGAGT GGAACGGCGT GGACCCTTAC 
ATGCGGGAGC AGCTCCGGGT CAAGATGGAG GATGGGGAGT TCTGGATGTC ATTCCGAGAC 
TTCATGCGTG AATTCACCCG CCTGGAGATC TGCAACCTGA CACCCGACGC GCTCAAGAGC 
CAGAGGTTCC GCAACTGGAA CACCACCCTG TATGAGGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGCAACTA CCCAGCCACT TTCTGGGTGA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGA CGGATGACCC GGACCCCGAC GACTACGGGG GTCGCGAGTC AGGCTGCAGC 
TTCCTGCTCG CCCTCATGCA GAAGCACCGC CGTCGGGAGC GCCGTTTCGG CCGTGACATG 
GAGACCATAG GCTTCGCTGT CTACGAGGTC CCTCCGGAGC TGATGGGCCA GCCGGCCGTG 
CATCTGAAGC GGGACTTCTT CCTGTCCAAC GCCTCCCGGG CCCGGTCCGA GCAGTTCATC 
AACCTGCGGG AGGTCAGCAC CCGCTTCCGC CTGCCGCCTG GGGAGTACGT GGTGGTGCCC 
TCCACCTTCG AGCCCAACAA GGAAGGTGAC TTTGTGCTGC GTTTCTTCTC AGAGAAGAGC 
GCAGGGACCC AAGAGCTGGA TGACCAGGTC CAGGCCAATC TCCCCGACGA GCAAGTGCTC 
TCAGAAGAGG AGATTGATGA GAACTTCAAG TCCCTCTTCA GACAACTGGC AGGGGAGGAC 
ATGGAGATCA GCGTCAAGGA GCTGCGGACC ATCCTCAACA GGATCATCAG CAAACACAAA 
GACCTGCGGA CCACGGGCTT CAGCCTGGAG TCCTGCCGCA GCATGGTCAA CCTCATGGAT 
CGCGACGGCA ATGGCAAACT GGGCCTGGTG GAGTTCAACA TCCTATGGAA CCGGATCCGG 
AATTACCTGT CCATCTTCCG GAAGTTTGAC CTGGACAAGT CGGGCAGCAT GAGTGCCTAT 
GAAATGCGGA TGGCCATTGA GTTTGCAGGC TTCAAGCTCA ACAAGAAGCT GTACGAGCTC 
ATTATCACCC GCTACTCGGA GCCCGACCTG GCCGTGGACT TCGACAACTT CGTGTGCTGC 
CTGGTGCGGC TGGAGACCAT GTTCCGGTTT TTCAAAACTC TGGACACTGA TCTGGATGGA 
GTGGTGACCT TTGACTTGTT TAAGTGGTTA CAGCTGACCA TGTTTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( XM_024986972.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842740.1 Bos taurus calpain 1 (CAPN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231831
Clone ID Related Accession (Same CDS sequence)	XM_024986972.1 , XM_024986973.1
Accession Version	XM_024986972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	calpain-1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG AGTTCATCAC TCCGGTGTAC TGCACCGGGG TGTCTGCACA AGTGCAGAAG 
CAGCGGGCCA AGGAGCTGGG CCTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTACGAGC AGCTGCGGGT TCACTGCCTG CAAAGAGGGG CCCTTTTCCG TGACGAGGCT 
TTCCCCCCAG TGCCCCAGAG CCTGGGCTTC AAGGAGCTGG GCCCCAACTC CTCCAAAACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAG CTGTTCTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GCACGGACAT CTGCCAGGGC GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATCGCCTCCC TTACCCTCAA TGACACCCTC CTGCACCGAG TAGTTCCACA TGGCCAAAGC 
TTCCAGGATG GCTACGCTGG CATCTTCCAT TTCCAGCTGT GGCAGTTTGG TGAGTGGGTG 
GATGTGGTGG TGGATGACCT GCTGCCCACC AAGGACGGGA AGCTGGTGTT TGTGCACTCT 
GCCCAAGGCA ACGAGTTCTG GAGCGCCCTG CTGGAGAAGG CCTATGCCAA GGTGAACGGC 
AGCTACGAGG CCCTCTCAGG AGGCAGCACA TCTGAGGGCT TTGAGGACTT CACCGGCGGA 
GTCACCGAGT GGTACGAGCT GCGCAAGGCG CCCAGCGACC TCTACAACAT CATCCTCAAG 
GCCCTGGAGC GTGGCTCCCT GCTGGGCTGC TCCATCGATA TCTCCAGCAT TCTGGACATG 
GAGGCTGTCA CCTTCAAGAA GCTGGTGAAG GGCCACGCCT ACTCTGTGAC CGGGGCCAAA 
CAGGTGAACT ACCAGGGCCA GATGGTGAAC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CAGGAGCCTG GAGTGACGGC TCCTCGGAGT GGAACGCCGT GGACCCTTAC 
ATGCGGGAGC AGCTCCGGGT CAAGATGGAG GATGGGGAGT TCTGGATGTC CTTCCGAGAC 
TTCATGCGTG AATTCACCCG CCTGGAGATC TGCAACCTGA CACCCGACGC GCTCAAGAGC 
CAGAGGTTCC GCAACTGGAA CACCACCCTG TACGAGGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGCAACTA CCCAGCCACT TTCTGGGTGA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGA CGGATGACCC GGACCCCGAC GACTACGGGG GTCGCGAGTC AGGCTGCAGC 
TTCCTGCTCG CCCTCATGCA GAAGCACCGC CGTCGGGAGC GCCGTTTCGG CCGTGACATG 
GAGACCATAG GCTTCGCTGT CTACGAGGTC CCTCCGGAGC TGATGGGCCA GCCGGCCGTG 
CATCTGAAGC GGGACTTCTT CCTGTCCAAC GCCTCCCGGG CCCGGTCCGA GCAGTTCATC 
AACCTGCGGG AGGTCAGCAC CCGCTTCCGC CTGCCGCCTG GGGAGTACGT GGTGGTGCCC 
TCCACCTTCG AGCCCAACAA GGAAGGTGAC TTTGTGCTGC GTTTCTTCTC AGAGAAGAGC 
GCAGGGACCC AAGAGCTGGA TGACCAGGTC CAGGCCAATC TCCCCGACGA GCAAGTGCTC 
TCAGAAGAGG AGATTGATGA GAACTTCAAG TCCCTCTTCA GACAACTGGC AGGGGAGGAC 
ATGGAGATCA GCGTCAAGGA GCTGCGGACC ATCCTCAACA GGATCATCAG CAAACACAAA 
GACCTGCGGA CCACGGGCTT CAGCCTGGAG TCCTGCCGCA GCATGGTCAA CCTCATGGAT 
CGCGACGGCA ATGGCAAACT GGGCCTGGTG GAGTTCAACA TCCTATGGAA CCGGATCCGG 
AATTACCTGT CCATCTTCCG GAAGTTTGAC CTGGACAAGT CGGGCAGCAT GAGTGCCTAT 
GAAATGCGGA TGGCCATTGA GTTTGCAGGC TTCAAGCTCA ACAAGAAGCT GTACGAGCTC 
ATTATCACCC GCTACTCGGA GCCCGACCTG GCCGTGGACT TCGACAACTT CGTGTGCTGC 
CTGGTGCGGC TGGAGACCAT GTTCCGGTTT TTCAAAACTC TGGACACTGA TCTGGATGGA 
GTGGTGACCT TTGACTTGTT TAAGTGGTTA CAGCTGACCA TGTTTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842740.1
CDS	138..2288
Translation	MAEEFITPVYCTGVSAQVQKQRAKELGLGRHENAIKYLGQDYEQLRVHCLQRGALFRDEAFPPVPQSLGFKELGPNSSKTYGIKWKRPTELFSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQDGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYNIILKALERGSLLGCSIDISSILDMEAVTFKKLVKGHAYSVTGAKQVNYQGQMVNLIRMRNPWGEVEWTGAWSDGSSEWNAVDPYMREQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSQRFRNWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEETDDPDPDDYGGRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYEVPPELMGQPAVHLKRDFFLSNASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTQELDDQVQANLPDEQVLSEEEIDENFKSLFRQLAGEDMEISVKELRTILNRIISKHKDLRTTGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIEFAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	XM_024986972.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 1 (CAPN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986972.1

ORF Insert Sequence:

ATGGCCGAGG AGTTCATCAC TCCGGTGTAC TGCACCGGGG TGTCTGCACA AGTGCAGAAG 
CAGCGGGCCA AGGAGCTGGG CCTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTACGAGC AGCTGCGGGT TCACTGCCTG CAAAGAGGGG CCCTTTTCCG TGACGAGGCT 
TTCCCCCCAG TGCCCCAGAG CCTGGGCTTC AAGGAGCTGG GCCCCAACTC CTCCAAAACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAG CTGTTCTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GCACGGACAT CTGCCAGGGC GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATCGCCTCCC TTACCCTCAA TGACACCCTC CTGCACCGAG TAGTTCCACA TGGCCAAAGC 
TTCCAGGATG GCTACGCTGG CATCTTCCAT TTCCAGCTGT GGCAGTTTGG TGAGTGGGTG 
GATGTGGTGG TGGATGACCT GCTGCCCACC AAGGACGGGA AGCTGGTGTT TGTGCACTCT 
GCCCAAGGCA ACGAGTTCTG GAGCGCCCTG CTGGAGAAGG CCTATGCCAA GGTGAACGGC 
AGCTACGAGG CCCTCTCAGG AGGCAGCACA TCTGAGGGCT TTGAGGACTT CACCGGCGGA 
GTCACCGAGT GGTACGAGCT GCGCAAGGCG CCCAGCGACC TCTACAACAT CATCCTCAAG 
GCCCTGGAGC GTGGCTCCCT GCTGGGCTGC TCCATCGATA TCTCCAGCAT TCTGGACATG 
GAGGCTGTCA CCTTCAAGAA GCTGGTGAAG GGCCACGCCT ACTCTGTGAC CGGGGCCAAA 
CAGGTGAACT ACCAGGGCCA GATGGTGAAC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CAGGAGCCTG GAGTGACGGC TCCTCGGAGT GGAACGCCGT GGACCCTTAC 
ATGCGGGAGC AGCTCCGGGT CAAGATGGAG GATGGGGAGT TCTGGATGTC CTTCCGAGAC 
TTCATGCGTG AATTCACCCG CCTGGAGATC TGCAACCTGA CACCCGACGC GCTCAAGAGC 
CAGAGGTTCC GCAACTGGAA CACCACCCTG TACGAGGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGCAACTA CCCAGCCACT TTCTGGGTGA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGA CGGATGACCC GGACCCCGAC GACTACGGGG GTCGCGAGTC AGGCTGCAGC 
TTCCTGCTCG CCCTCATGCA GAAGCACCGC CGTCGGGAGC GCCGTTTCGG CCGTGACATG 
GAGACCATAG GCTTCGCTGT CTACGAGGTC CCTCCGGAGC TGATGGGCCA GCCGGCCGTG 
CATCTGAAGC GGGACTTCTT CCTGTCCAAC GCCTCCCGGG CCCGGTCCGA GCAGTTCATC 
AACCTGCGGG AGGTCAGCAC CCGCTTCCGC CTGCCGCCTG GGGAGTACGT GGTGGTGCCC 
TCCACCTTCG AGCCCAACAA GGAAGGTGAC TTTGTGCTGC GTTTCTTCTC AGAGAAGAGC 
GCAGGGACCC AAGAGCTGGA TGACCAGGTC CAGGCCAATC TCCCCGACGA GCAAGTGCTC 
TCAGAAGAGG AGATTGATGA GAACTTCAAG TCCCTCTTCA GACAACTGGC AGGGGAGGAC 
ATGGAGATCA GCGTCAAGGA GCTGCGGACC ATCCTCAACA GGATCATCAG CAAACACAAA 
GACCTGCGGA CCACGGGCTT CAGCCTGGAG TCCTGCCGCA GCATGGTCAA CCTCATGGAT 
CGCGACGGCA ATGGCAAACT GGGCCTGGTG GAGTTCAACA TCCTATGGAA CCGGATCCGG 
AATTACCTGT CCATCTTCCG GAAGTTTGAC CTGGACAAGT CGGGCAGCAT GAGTGCCTAT 
GAAATGCGGA TGGCCATTGA GTTTGCAGGC TTCAAGCTCA ACAAGAAGCT GTACGAGCTC 
ATTATCACCC GCTACTCGGA GCCCGACCTG GCCGTGGACT TCGACAACTT CGTGTGCTGC 
CTGGTGCGGC TGGAGACCAT GTTCCGGTTT TTCAAAACTC TGGACACTGA TCTGGATGGA 
GTGGTGACCT TTGACTTGTT TAAGTGGTTA CAGCTGACCA TGTTTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN1 ( XM_024986973.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842741.1 Bos taurus calpain 1 (CAPN1), transcript variant X2, mRNA.

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CloneID	OBa231831
Clone ID Related Accession (Same CDS sequence)	XM_024986972.1 , XM_024986973.1
Accession Version	XM_024986973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	calpain-1 catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG AGTTCATCAC TCCGGTGTAC TGCACCGGGG TGTCTGCACA AGTGCAGAAG 
CAGCGGGCCA AGGAGCTGGG CCTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTACGAGC AGCTGCGGGT TCACTGCCTG CAAAGAGGGG CCCTTTTCCG TGACGAGGCT 
TTCCCCCCAG TGCCCCAGAG CCTGGGCTTC AAGGAGCTGG GCCCCAACTC CTCCAAAACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAG CTGTTCTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GCACGGACAT CTGCCAGGGC GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATCGCCTCCC TTACCCTCAA TGACACCCTC CTGCACCGAG TAGTTCCACA TGGCCAAAGC 
TTCCAGGATG GCTACGCTGG CATCTTCCAT TTCCAGCTGT GGCAGTTTGG TGAGTGGGTG 
GATGTGGTGG TGGATGACCT GCTGCCCACC AAGGACGGGA AGCTGGTGTT TGTGCACTCT 
GCCCAAGGCA ACGAGTTCTG GAGCGCCCTG CTGGAGAAGG CCTATGCCAA GGTGAACGGC 
AGCTACGAGG CCCTCTCAGG AGGCAGCACA TCTGAGGGCT TTGAGGACTT CACCGGCGGA 
GTCACCGAGT GGTACGAGCT GCGCAAGGCG CCCAGCGACC TCTACAACAT CATCCTCAAG 
GCCCTGGAGC GTGGCTCCCT GCTGGGCTGC TCCATCGATA TCTCCAGCAT TCTGGACATG 
GAGGCTGTCA CCTTCAAGAA GCTGGTGAAG GGCCACGCCT ACTCTGTGAC CGGGGCCAAA 
CAGGTGAACT ACCAGGGCCA GATGGTGAAC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CAGGAGCCTG GAGTGACGGC TCCTCGGAGT GGAACGCCGT GGACCCTTAC 
ATGCGGGAGC AGCTCCGGGT CAAGATGGAG GATGGGGAGT TCTGGATGTC CTTCCGAGAC 
TTCATGCGTG AATTCACCCG CCTGGAGATC TGCAACCTGA CACCCGACGC GCTCAAGAGC 
CAGAGGTTCC GCAACTGGAA CACCACCCTG TACGAGGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGCAACTA CCCAGCCACT TTCTGGGTGA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGA CGGATGACCC GGACCCCGAC GACTACGGGG GTCGCGAGTC AGGCTGCAGC 
TTCCTGCTCG CCCTCATGCA GAAGCACCGC CGTCGGGAGC GCCGTTTCGG CCGTGACATG 
GAGACCATAG GCTTCGCTGT CTACGAGGTC CCTCCGGAGC TGATGGGCCA GCCGGCCGTG 
CATCTGAAGC GGGACTTCTT CCTGTCCAAC GCCTCCCGGG CCCGGTCCGA GCAGTTCATC 
AACCTGCGGG AGGTCAGCAC CCGCTTCCGC CTGCCGCCTG GGGAGTACGT GGTGGTGCCC 
TCCACCTTCG AGCCCAACAA GGAAGGTGAC TTTGTGCTGC GTTTCTTCTC AGAGAAGAGC 
GCAGGGACCC AAGAGCTGGA TGACCAGGTC CAGGCCAATC TCCCCGACGA GCAAGTGCTC 
TCAGAAGAGG AGATTGATGA GAACTTCAAG TCCCTCTTCA GACAACTGGC AGGGGAGGAC 
ATGGAGATCA GCGTCAAGGA GCTGCGGACC ATCCTCAACA GGATCATCAG CAAACACAAA 
GACCTGCGGA CCACGGGCTT CAGCCTGGAG TCCTGCCGCA GCATGGTCAA CCTCATGGAT 
CGCGACGGCA ATGGCAAACT GGGCCTGGTG GAGTTCAACA TCCTATGGAA CCGGATCCGG 
AATTACCTGT CCATCTTCCG GAAGTTTGAC CTGGACAAGT CGGGCAGCAT GAGTGCCTAT 
GAAATGCGGA TGGCCATTGA GTTTGCAGGC TTCAAGCTCA ACAAGAAGCT GTACGAGCTC 
ATTATCACCC GCTACTCGGA GCCCGACCTG GCCGTGGACT TCGACAACTT CGTGTGCTGC 
CTGGTGCGGC TGGAGACCAT GTTCCGGTTT TTCAAAACTC TGGACACTGA TCTGGATGGA 
GTGGTGACCT TTGACTTGTT TAAGTGGTTA CAGCTGACCA TGTTTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842741.1
CDS	157..2307
Translation	MAEEFITPVYCTGVSAQVQKQRAKELGLGRHENAIKYLGQDYEQLRVHCLQRGALFRDEAFPPVPQSLGFKELGPNSSKTYGIKWKRPTELFSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQDGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDLYNIILKALERGSLLGCSIDISSILDMEAVTFKKLVKGHAYSVTGAKQVNYQGQMVNLIRMRNPWGEVEWTGAWSDGSSEWNAVDPYMREQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSQRFRNWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEETDDPDPDDYGGRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYEVPPELMGQPAVHLKRDFFLSNASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTQELDDQVQANLPDEQVLSEEEIDENFKSLFRQLAGEDMEISVKELRTILNRIISKHKDLRTTGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIEFAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA

Target ORF information:

RefSeq Version	XM_024986973.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 1 (CAPN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986973.1

ORF Insert Sequence:

ATGGCCGAGG AGTTCATCAC TCCGGTGTAC TGCACCGGGG TGTCTGCACA AGTGCAGAAG 
CAGCGGGCCA AGGAGCTGGG CCTGGGCCGC CATGAAAATG CCATCAAGTA CCTGGGCCAG 
GATTACGAGC AGCTGCGGGT TCACTGCCTG CAAAGAGGGG CCCTTTTCCG TGACGAGGCT 
TTCCCCCCAG TGCCCCAGAG CCTGGGCTTC AAGGAGCTGG GCCCCAACTC CTCCAAAACC 
TATGGCATCA AGTGGAAGCG TCCCACGGAG CTGTTCTCAA ACCCCCAGTT CATCGTGGAT 
GGAGCCACCC GCACGGACAT CTGCCAGGGC GCACTGGGGG ACTGTTGGCT CCTGGCTGCC 
ATCGCCTCCC TTACCCTCAA TGACACCCTC CTGCACCGAG TAGTTCCACA TGGCCAAAGC 
TTCCAGGATG GCTACGCTGG CATCTTCCAT TTCCAGCTGT GGCAGTTTGG TGAGTGGGTG 
GATGTGGTGG TGGATGACCT GCTGCCCACC AAGGACGGGA AGCTGGTGTT TGTGCACTCT 
GCCCAAGGCA ACGAGTTCTG GAGCGCCCTG CTGGAGAAGG CCTATGCCAA GGTGAACGGC 
AGCTACGAGG CCCTCTCAGG AGGCAGCACA TCTGAGGGCT TTGAGGACTT CACCGGCGGA 
GTCACCGAGT GGTACGAGCT GCGCAAGGCG CCCAGCGACC TCTACAACAT CATCCTCAAG 
GCCCTGGAGC GTGGCTCCCT GCTGGGCTGC TCCATCGATA TCTCCAGCAT TCTGGACATG 
GAGGCTGTCA CCTTCAAGAA GCTGGTGAAG GGCCACGCCT ACTCTGTGAC CGGGGCCAAA 
CAGGTGAACT ACCAGGGCCA GATGGTGAAC CTGATCCGGA TGCGGAACCC CTGGGGCGAG 
GTGGAGTGGA CAGGAGCCTG GAGTGACGGC TCCTCGGAGT GGAACGCCGT GGACCCTTAC 
ATGCGGGAGC AGCTCCGGGT CAAGATGGAG GATGGGGAGT TCTGGATGTC CTTCCGAGAC 
TTCATGCGTG AATTCACCCG CCTGGAGATC TGCAACCTGA CACCCGACGC GCTCAAGAGC 
CAGAGGTTCC GCAACTGGAA CACCACCCTG TACGAGGGCA CCTGGCGGCG GGGGAGCACC 
GCGGGGGGCT GCCGCAACTA CCCAGCCACT TTCTGGGTGA ACCCCCAGTT CAAGATCCGG 
CTGGAGGAGA CGGATGACCC GGACCCCGAC GACTACGGGG GTCGCGAGTC AGGCTGCAGC 
TTCCTGCTCG CCCTCATGCA GAAGCACCGC CGTCGGGAGC GCCGTTTCGG CCGTGACATG 
GAGACCATAG GCTTCGCTGT CTACGAGGTC CCTCCGGAGC TGATGGGCCA GCCGGCCGTG 
CATCTGAAGC GGGACTTCTT CCTGTCCAAC GCCTCCCGGG CCCGGTCCGA GCAGTTCATC 
AACCTGCGGG AGGTCAGCAC CCGCTTCCGC CTGCCGCCTG GGGAGTACGT GGTGGTGCCC 
TCCACCTTCG AGCCCAACAA GGAAGGTGAC TTTGTGCTGC GTTTCTTCTC AGAGAAGAGC 
GCAGGGACCC AAGAGCTGGA TGACCAGGTC CAGGCCAATC TCCCCGACGA GCAAGTGCTC 
TCAGAAGAGG AGATTGATGA GAACTTCAAG TCCCTCTTCA GACAACTGGC AGGGGAGGAC 
ATGGAGATCA GCGTCAAGGA GCTGCGGACC ATCCTCAACA GGATCATCAG CAAACACAAA 
GACCTGCGGA CCACGGGCTT CAGCCTGGAG TCCTGCCGCA GCATGGTCAA CCTCATGGAT 
CGCGACGGCA ATGGCAAACT GGGCCTGGTG GAGTTCAACA TCCTATGGAA CCGGATCCGG 
AATTACCTGT CCATCTTCCG GAAGTTTGAC CTGGACAAGT CGGGCAGCAT GAGTGCCTAT 
GAAATGCGGA TGGCCATTGA GTTTGCAGGC TTCAAGCTCA ACAAGAAGCT GTACGAGCTC 
ATTATCACCC GCTACTCGGA GCCCGACCTG GCCGTGGACT TCGACAACTT CGTGTGCTGC 
CTGGTGCGGC TGGAGACCAT GTTCCGGTTT TTCAAAACTC TGGACACTGA TCTGGATGGA 
GTGGTGACCT TTGACTTGTT TAAGTGGTTA CAGCTGACCA TGTTTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of single nucleotide polymorphisms in CAPN1, CAST and MB genes with meat color of Brahman and crossbreed cattle.
Meat science11744-9(2016 Jul)
Castro S,R?os M,Ortiz Y,Manrique C,Jim?nez A,Ariza F

CAPN1, CAST, and DGAT1 genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in a beef cattle population selected for haplotype and allele equalization.
Journal of animal science92(12)5382-93(2014 Dec)
Tait RG Jr,Shackelford SD,Wheeler TL,King DA,Keele JW,Casas E,Smith TP,Bennett GL

Understanding the interaction determinants of CAPN1 inhibition by CAST4 from bovines using molecular modeling techniques.
Molecules (Basel, Switzerland)19(9)14316-51(2014 Sep)
Chai HH,Lim D,Jung E,Choi BH,Cho YM

Bovine mu-calpain (CAPN1) gene: new SNP within intron 14.
Journal of applied genetics45(4)457-60(2004)
Juszczuk-Kubiak E,Sakowski T,Flisikowski K,Wicinska K,Oprzadek J,Rosochacki SJ

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CAPN1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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