ACTR3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTR3
Entrez Gene ID	281597
Full Name	ARP3 actin related protein 3 homolog
General protein information	Preferred Names ARP3 actin related protein 3 homolog Names actin-related protein 3 ARP3 (actin-related protein 3, yeast) homolog actin-2 actin-like protein 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174226.2	NP_776651.1	actin-related protein 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Actr3	NP_001192314.1
Saccharomyces cerevisiae (baker's yeast)	ARP3	NP_012599.3
Kluyveromyces lactis	KLLA0B13904g	XP_452150.1
Eremothecium gossypii	AGOS_AFR419C	NP_985966.1
Schizosaccharomyces pombe (fission yeast)	arp3	NP_592898.1
Bos taurus (cattle)	ACTR3	NP_776651.1
Canis lupus familiaris (dog)	ACTR3B	XP_853309.2
Rattus norvegicus (Norway rat)	Actr3	NP_112330.1
Homo sapiens (human)	ACTR3	NP_005712.1
Pan troglodytes (chimpanzee)	ACTR3	XP_001154210.1
Danio rerio (zebrafish)	actr3	NP_001003944.1
Drosophila melanogaster (fruit fly)	Arp3	NP_523968.1
Caenorhabditis elegans (roundworm)	arx-1	NP_491066.1
Neurospora crassa	NCU01756	XP_957179.2
Gallus gallus (chicken)	ACTR3	NP_989638.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005110	XP_313987.1
Magnaporthe oryzae (rice blast fungus)	MGG_03879	XP_003720000.1
Xenopus tropicalis (tropical clawed frog)	actr3	NP_989368.1

Gene Ontology
Bibliography

Related articles in PubMed

Collapsin Response Mediator Protein-1 Regulates Arp2/3-dependent Actin Assembly.
Yu-Kemp HC, Brieher WM
The Journal of biological chemistry291(2)658-64(2016 Jan)

Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites.
Jurgenson CT, Pollard TD
Acta crystallographica. Section F, Structural biology communications71(Pt 9)1161-8(2015 Sep)

Structural basis for regulation of Arp2/3 complex by GMF.
Luan Q, Nolen BJ
Nature structural & molecular biology20(9)1062-8(2013 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Focal adhesion kinase controls actin assembly via a FERM-mediated interaction with the Arp2/3 complex.
Serrels B, Serrels A, Brunton VG, Holt M, McLean GW, Gray CH, Jones GE, Frame MC
Nature cell biology9(9)1046-56(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate an important role for CRMP-1 in Listeria actin comet tail formation and open the possibility that CRMP-1 controls cell motility by modulating Arp2/3 activation.
Title: Collapsin Response Mediator Protein-1 Regulates Arp2/3-dependent Actin Assembly.

crystal structure of Arp2/3 complex
Title: Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites.

The GMF-Arp2 interface reveals how the ADF-H actin-binding domain in GMF is exploited to specifically recognize Arp2/3 complex and not actin.
Title: Structural basis for regulation of Arp2/3 complex by GMF.

domain rearrangements of Arp2 and Arp3 result in a closed conformational state consistent with an "actin-dimer" model for the active state
Title: Visualizing Arp2/3 complex activation mediated by binding of ATP and WASp using structural mass spectrometry.

WASp stabilizes p35-dependent closure of the complex, holding Arp2 and Arp3 closer together to nucleate an actin filament.
Title: Conformational changes in the Arp2/3 complex leading to actin nucleation.

The following ACTR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01334	NM_174226.2 Latest version!	Bos taurus actin related protein 3 (ACTR3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTR3 ( NM_174226.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776651.1 Bos taurus actin related protein 3 (ACTR3), mRNA.

Price & Availability↑

CloneID	OBa01334
Clone ID Related Accession (Same CDS sequence)	NM_174226.2
Accession Version	NM_174226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	actin-related protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D12816.1. On Jun 3, 2003 this sequence version replaced NM_174226.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12816.1, BC123582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGAC GGCTGCCGGC CTGTGTGGTG GACTGTGGCA CGGGGTATAC AAAATTAGGA 
TATGCTGGAA ATACGGAACC ACAGTTTATC ATCCCTTCCT GTATTGCTAT TAAAGAGTCA 
GCAAAAGTGG GAGATCAAGC TCAAAGGAGG GTGATGAAAG GTGTTGACGA CCTAGACTTC 
TTCATTGGTG ATGAAGCAAT AGAAAAACCT ACATATGCAA CAAAGTGGCC AATCCGCCAT 
GGTATAGTTG AAGACTGGGA CTTGATGGAA AGGTTTATGG AGCAAGTCAT CTTTAAATAT 
TTAAGGGCGG AACCTGAAGA TCATTATTTT CTTTTGACTG AACCTCCACT GAATACTCCA 
GAAAACAGGG AATATACTGC TGAAATAATG TTTGAGTCTT TCAATGTTCC TGGCTTGTAC 
ATTGCTGTAC AGGCCGTTCT TGCCTTAGCT GCGTCTTGGA CCTCTAGACA AGTAGGAGAA 
CGGACGTTGA CCGGTACGGT AATAGACAGT GGAGACGGTG TCACTCATGT CATCCCTGTG 
GCTGAAGGGT ATGTGATTGG CAGCTGTATT AAGCACATTC CCATCGCAGG ACGAGATATA 
ACATATTTTA TTCAGCAATT GTTGAGAGAC CGAGAAGTAG GAATTCCTCC AGAGCAGTCC 
TTGGAAACTG CTAAGGCAGT GAAGGAGCGC TATAGTTATG TCTGCCCAGA CTTAGTAAAA 
GAATTTAACA AGTATGATAC AGATGGATCA AAGTGGATTA AACAGTATAC TGGAATCAAT 
GCTATCTCAA AGAAAGAGTT TTCCATTGAT GTTGGATATG AGAGATTCTT GGGACCTGAA 
ATTTTTTTTC ATCCAGAGTT TGCTAACCCA GACTTTACCC AGCCTATCTC AGAAGTTGTA 
GATGAAGTAA TTCAGAATTG TCCTATCGAT GTCAGACGTC CTCTCTACAA GAATATTGTC 
CTCTCTGGAG GTTCAACCAT GTTCAGGGAC TTTGGACGTC GTTTGCAAAG AGATTTGAAA 
AGAACTGTAG ATGCCAGGCT GAAATTAAGT GAGGAATTGA GTGGTGGTAG ATTGAAGCCA 
AAACCTATTG ATGTACAAGT CATTACACAT CACATGCAAC GATACGCAGT TTGGTTTGGA 
GGATCTATGC TGGCTTCCAC ACCTGAGTTC TACCAAGTAT GCCACACCAA AAAGGATTAT 
GAAGAAATTG GACCTAGCAT TTGTCGTCAC AATCCAGTGT TTGGAGTCAT GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776651.1
CDS	119..1375
Translation	MAGRLPACVVDCGTGYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRVMKGVDDLDFFIGDEAIEKPTYATKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS

Target ORF information:

RefSeq Version	NM_174226.2
Organism	Bos taurus(cattle)
Definition	Bos taurus actin related protein 3 (ACTR3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174226.2

ORF Insert Sequence:

ATGGCGGGAC GGCTGCCGGC CTGTGTGGTG GACTGTGGCA CGGGGTATAC AAAATTAGGA 
TATGCTGGAA ATACGGAACC ACAGTTTATC ATCCCTTCCT GTATTGCTAT TAAAGAGTCA 
GCAAAAGTGG GAGATCAAGC TCAAAGGAGG GTGATGAAAG GTGTTGACGA CCTAGACTTC 
TTCATTGGTG ATGAAGCAAT AGAAAAACCT ACATATGCAA CAAAGTGGCC AATCCGCCAT 
GGTATAGTTG AAGACTGGGA CTTGATGGAA AGGTTTATGG AGCAAGTCAT CTTTAAATAT 
TTAAGGGCGG AACCTGAAGA TCATTATTTT CTTTTGACTG AACCTCCACT GAATACTCCA 
GAAAACAGGG AATATACTGC TGAAATAATG TTTGAGTCTT TCAATGTTCC TGGCTTGTAC 
ATTGCTGTAC AGGCCGTTCT TGCCTTAGCT GCGTCTTGGA CCTCTAGACA AGTAGGAGAA 
CGGACGTTGA CCGGTACGGT AATAGACAGT GGAGACGGTG TCACTCATGT CATCCCTGTG 
GCTGAAGGGT ATGTGATTGG CAGCTGTATT AAGCACATTC CCATCGCAGG ACGAGATATA 
ACATATTTTA TTCAGCAATT GTTGAGAGAC CGAGAAGTAG GAATTCCTCC AGAGCAGTCC 
TTGGAAACTG CTAAGGCAGT GAAGGAGCGC TATAGTTATG TCTGCCCAGA CTTAGTAAAA 
GAATTTAACA AGTATGATAC AGATGGATCA AAGTGGATTA AACAGTATAC TGGAATCAAT 
GCTATCTCAA AGAAAGAGTT TTCCATTGAT GTTGGATATG AGAGATTCTT GGGACCTGAA 
ATTTTTTTTC ATCCAGAGTT TGCTAACCCA GACTTTACCC AGCCTATCTC AGAAGTTGTA 
GATGAAGTAA TTCAGAATTG TCCTATCGAT GTCAGACGTC CTCTCTACAA GAATATTGTC 
CTCTCTGGAG GTTCAACCAT GTTCAGGGAC TTTGGACGTC GTTTGCAAAG AGATTTGAAA 
AGAACTGTAG ATGCCAGGCT GAAATTAAGT GAGGAATTGA GTGGTGGTAG ATTGAAGCCA 
AAACCTATTG ATGTACAAGT CATTACACAT CACATGCAAC GATACGCAGT TTGGTTTGGA 
GGATCTATGC TGGCTTCCAC ACCTGAGTTC TACCAAGTAT GCCACACCAA AAAGGATTAT 
GAAGAAATTG GACCTAGCAT TTGTCGTCAC AATCCAGTGT TTGGAGTCAT GTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Collapsin Response Mediator Protein-1 Regulates Arp2/3-dependent Actin Assembly.
The Journal of biological chemistry291(2)658-64(2016 Jan)
Yu-Kemp HC,Brieher WM

Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites.
Acta crystallographica. Section F, Structural biology communications71(Pt 9)1161-8(2015 Sep)
Jurgenson CT,Pollard TD

Structural basis for regulation of Arp2/3 complex by GMF.
Nature structural & molecular biology20(9)1062-8(2013 Sep)
Luan Q,Nolen BJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Focal adhesion kinase controls actin assembly via a FERM-mediated interaction with the Arp2/3 complex.
Nature cell biology9(9)1046-56(2007 Sep)
Serrels B,Serrels A,Brunton VG,Holt M,McLean GW,Gray CH,Jones GE,Frame MC

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ACTR3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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