EZR cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EZR
Entrez Gene ID	281574
Full Name	ezrin
Synonyms	VIL2
General protein information	Preferred Names ezrin Names ezrin cytovillin p81 villin 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010800726.3	XP_010799028.1	ezrin isoform X1
XM_010800726.2	XP_010799028.1	ezrin isoform X1
NM_174217.2	NP_776642.1	ezrin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EZR	XP_541173.2
	EZR	XP_002803901.1
Pan troglodytes (chimpanzee)	EZR	XP_003311612.1
Xenopus tropicalis (tropical clawed frog)	ezr	NP_001025629.1
Mus musculus (house mouse)	Ezr	NP_033536.2
Rattus norvegicus (Norway rat)	Ezr	NP_062230.1
Gallus gallus (chicken)	EZR	NP_990216.1
Danio rerio (zebrafish)	ezra	NP_001018326.1
Homo sapiens (human)	EZR	NP_003370.2
Bos taurus (cattle)	EZR	NP_776642.1
Danio rerio (zebrafish)	ezrb	NP_001025456.1

Gene Ontology
Bibliography

Related articles in PubMed

Sphingosine 1-phosphate activation of ERM contributes to vascular calcification.
Morris TG, Borland SJ, Clarke CJ, Wilson C, Hannun YA, Ohanian V, Canfield AE, Ohanian J
Journal of lipid research59(1)69-78(2018 Jan)

Changes in endometrial ezrin and cytokeratin 18 expression during bovine implantation and in caruncular endometrial spheroids in vitro.
Haeger JD, Hambruch N, Dantzer V, Hoelker M, Schellander K, Klisch K, Pfarrer C
Placenta36(8)821-31(2015 Aug)

An ezrin/calpain/PI3K/AMPK/eNOSs1179 signaling cascade mediating VEGF-dependent endothelial nitric oxide production.
Youn JY, Wang T, Cai H
Circulation research104(1)50-9(2009 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Ezrin and osteonectin, two proteins associated with cell shape and growth, are enriched in the locus coeruleus.
Bergson C, Zhao H, Saijoh K, Duman RS, Nestler EJ
Molecular and cellular neurosciences4(1)64-73(1993 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ezrin and CK18 are downregulated during implantation in cattle. The expression changes represent a temporal depolarization, which could be important for an establishment of bovine pregnancy.
Title: Changes in endometrial ezrin and cytokeratin 18 expression during bovine implantation and in caruncular endometrial spheroids in vitro.

Ezrin-dependent, membrane-specific translocation and activation of calpain by VEGF precedes AMPK and AKT-dependent phosphorylation of eNOSs1179 and production of NO.
Title: An ezrin/calpain/PI3K/AMPK/eNOSs1179 signaling cascade mediating VEGF-dependent endothelial nitric oxide production.

The following EZR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EZR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42177	NM_174217.2 Latest version!	Bos taurus ezrin (EZR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa42177	XM_010800726.3 Latest version!	Bos taurus ezrin (EZR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa42177	XM_010800726.2	Bos taurus ezrin (EZR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EZR ( NM_174217.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776642.1 Bos taurus ezrin (EZR), mRNA.

Price & Availability↑

CloneID	OBa42177
Clone ID Related Accession (Same CDS sequence)	XM_010800726.3 , XM_010800726.2 , NM_174217.2
Accession Version	NM_174217.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Bos taurus(cattle)
Product	ezrin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102573.1. On Aug 28, 2005 this sequence version replaced NM_174217.1. ##Evidence-Data-START## Transcript exon combination :: BC102573.1, M98498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

ATGCCGAAAC CGATCAACGT CCGAGTGACC ACCATGGATG CGGAGCTGGA GTTTGCCATC 
CAGCCAAACA CCACAGGAAA GCAGCTTTTT GATCAGGTGG TTAAGACCAT CGGCCTTAGG 
GAAGTGTGGT ACTTTGGCCT CCAGTATGTG GATAACAAAG GATTTCCTAC ATGGTTGAAA 
CTTGATAAAA AGGTGTCCGC CCAGGAGGTC AGGAAGGAGA GCCCGCTGCA GTTCAAGTTC 
CGGGCCAAGT TCTACCCCGA GGACGTGGCC GAGGAGCTCA TCCAGGACAT CACGCAGAAG 
CTCTTCTTCC TGCAAGTGAA GGAGGGCATC CTCAGCGACG AGATCTACTG CCCGCCCGAG 
ACCGCCGTGC TCCTGGGCTC CTACGCCGTG CAGGCCAAGT TCGGCGACTA CAACAAGGAG 
CTGCACAAGG CGGGGTACCT CGGCTCCGAG CGGCTCATCC CCCAGAGAGT CATGGACCAG 
CACAAGCTCA CCAGGGACCA GTGGGAGGAC CGGATCCAGG TGTGGCATGC AGAACACCGC 
GGGATGCTCA AGGACAGCGC CATGCTGGAA TACCTGAAGA TCGCCCAGGA CCTGGAAATG 
TACGGGATCA ACTATTTCGA GATAAAAAAT AAGAAAGGAA CAGACCTTTG GCTCGGCGTT 
GATGCCCTTG GACTAAATAT CTATGAGAAA GACGATAAGT TGACCCCAAA GATTGGCTTC 
CCATGGAGCG AGATCAGGAA CATCTCTTTC AATGACAAGA AGTTTGTCAT CAAGCCCATC 
GACAAGAAGG CTCCTGACTT CGTGTTCTAC GCCCCCCGCC TGCGGATCAA CAAGCGGATC 
CTGCAGCTCT GCATGGGGAA CCATGAGCTG TACATGCGCC GCCGGAAGCC CGACACCATT 
GAGGTGCAGC AGATGAAGGC CCAGGCCCGG GAGGAGAAGC ACCAGAAGCA GCTGGAGAGG 
CAGCAGTTGG AAACCGAGAA GAAGAGGAGA GAAACCGTGG AGCGAGAAAA AGAGCAGATG 
ATGCGTGAGA AGGAGGAGCT GATGCTCAGG CTTCAGGACT ACGAGGAAAA AACCAGGAAG 
GCTGAGAAAG AGCTCTCCGA CCAGATCCAG AGAGCGCTGA AGCTGGAGGA GGAGAGGAAG 
CGGGCCCAGG AGGAGGCGGG GCGCCTGGAG GCTGACCGCC TGGCCGCGCT GCGTGCCAAG 
GAGGAGCTGG AGAGGCAGGC AGCCGACCAG ATCAAGAGCC AGGAGCAGCT GGCCACAGAG 
CTCGCCGAGT ACACCGCCAA GATCGCCCTC CTGGAGGAGG CACGGCGGCG CAAGGAGAAT 
GAAGTGGAAG AGTGGCAGCT CCGGGCCAAG GAAGCCCAGG ATGACCTGGT GAAGACCCGG 
GAGGAGCTGC ACCTGGTGAT GACCGCGCCG CCGCCGCCAC CGGTGTACGA GCCGGTGAAC 
TACCACGTGC ATGAGGGCCC GCAGGAGGAG GGCACGGAGC TGAGCGCCGA GCTGTCCAGC 
GAGGGCATCC TGGACGACCG CAACGAGGAG AAGCGCATCA CCGAGGCCGA GAAGAACGAG 
CGCGTGCAGC GGCAGCTGAT GACTCTGACC AGTGAGCTGT CCCAGGCCAG AGACGAGAAC 
AAGCGGACCC ACAACGACAT CATCCACAAT GAGAACATGA GGCAAGGCCG GGACAAGTAC 
AAGACGCTGC GGCAGATCCG GCAAGGCAAC ACCAAGCAGC GCATCGACGA GTTCGAGGCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776642.1
CDS	175..1920
Translation	MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEVRKESPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKELHKAGYLGSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTRKAEKELSDQIQRALKLEEERKRAQEEAGRLEADRLAALRAKEELERQAADQIKSQEQLATELAEYTAKIALLEEARRRKENEVEEWQLRAKEAQDDLVKTREELHLVMTAPPPPPVYEPVNYHVHEGPQEEGTELSAELSSEGILDDRNEEKRITEAEKNERVQRQLMTLTSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAM

Target ORF information:

RefSeq Version	NM_174217.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ezrin (EZR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174217.2

ORF Insert Sequence:

ATGCCGAAAC CGATCAACGT CCGAGTGACC ACCATGGATG CGGAGCTGGA GTTTGCCATC 
CAGCCAAACA CCACAGGAAA GCAGCTTTTT GATCAGGTGG TTAAGACCAT CGGCCTTAGG 
GAAGTGTGGT ACTTTGGCCT CCAGTATGTG GATAACAAAG GATTTCCTAC ATGGTTGAAA 
CTTGATAAAA AGGTGTCCGC CCAGGAGGTC AGGAAGGAGA GCCCGCTGCA GTTCAAGTTC 
CGGGCCAAGT TCTACCCCGA GGACGTGGCC GAGGAGCTCA TCCAGGACAT CACGCAGAAG 
CTCTTCTTCC TGCAAGTGAA GGAGGGCATC CTCAGCGACG AGATCTACTG CCCGCCCGAG 
ACCGCCGTGC TCCTGGGCTC CTACGCCGTG CAGGCCAAGT TCGGCGACTA CAACAAGGAG 
CTGCACAAGG CGGGGTACCT CGGCTCCGAG CGGCTCATCC CCCAGAGAGT CATGGACCAG 
CACAAGCTCA CCAGGGACCA GTGGGAGGAC CGGATCCAGG TGTGGCATGC AGAACACCGC 
GGGATGCTCA AGGACAGCGC CATGCTGGAA TACCTGAAGA TCGCCCAGGA CCTGGAAATG 
TACGGGATCA ACTATTTCGA GATAAAAAAT AAGAAAGGAA CAGACCTTTG GCTCGGCGTT 
GATGCCCTTG GACTAAATAT CTATGAGAAA GACGATAAGT TGACCCCAAA GATTGGCTTC 
CCATGGAGCG AGATCAGGAA CATCTCTTTC AATGACAAGA AGTTTGTCAT CAAGCCCATC 
GACAAGAAGG CTCCTGACTT CGTGTTCTAC GCCCCCCGCC TGCGGATCAA CAAGCGGATC 
CTGCAGCTCT GCATGGGGAA CCATGAGCTG TACATGCGCC GCCGGAAGCC CGACACCATT 
GAGGTGCAGC AGATGAAGGC CCAGGCCCGG GAGGAGAAGC ACCAGAAGCA GCTGGAGAGG 
CAGCAGTTGG AAACCGAGAA GAAGAGGAGA GAAACCGTGG AGCGAGAAAA AGAGCAGATG 
ATGCGTGAGA AGGAGGAGCT GATGCTCAGG CTTCAGGACT ACGAGGAAAA AACCAGGAAG 
GCTGAGAAAG AGCTCTCCGA CCAGATCCAG AGAGCGCTGA AGCTGGAGGA GGAGAGGAAG 
CGGGCCCAGG AGGAGGCGGG GCGCCTGGAG GCTGACCGCC TGGCCGCGCT GCGTGCCAAG 
GAGGAGCTGG AGAGGCAGGC AGCCGACCAG ATCAAGAGCC AGGAGCAGCT GGCCACAGAG 
CTCGCCGAGT ACACCGCCAA GATCGCCCTC CTGGAGGAGG CACGGCGGCG CAAGGAGAAT 
GAAGTGGAAG AGTGGCAGCT CCGGGCCAAG GAAGCCCAGG ATGACCTGGT GAAGACCCGG 
GAGGAGCTGC ACCTGGTGAT GACCGCGCCG CCGCCGCCAC CGGTGTACGA GCCGGTGAAC 
TACCACGTGC ATGAGGGCCC GCAGGAGGAG GGCACGGAGC TGAGCGCCGA GCTGTCCAGC 
GAGGGCATCC TGGACGACCG CAACGAGGAG AAGCGCATCA CCGAGGCCGA GAAGAACGAG 
CGCGTGCAGC GGCAGCTGAT GACTCTGACC AGTGAGCTGT CCCAGGCCAG AGACGAGAAC 
AAGCGGACCC ACAACGACAT CATCCACAAT GAGAACATGA GGCAAGGCCG GGACAAGTAC 
AAGACGCTGC GGCAGATCCG GCAAGGCAAC ACCAAGCAGC GCATCGACGA GTTCGAGGCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EZR ( XM_010800726.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799028.1 Bos taurus ezrin (EZR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa42177
Clone ID Related Accession (Same CDS sequence)	XM_010800726.3 , XM_010800726.2 , NM_174217.2
Accession Version	XM_010800726.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ezrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAAC CGATCAACGT CCGAGTGACC ACCATGGATG CGGAGCTGGA GTTTGCCATC 
CAGCCAAACA CCACAGGAAA GCAGCTTTTT GATCAGGTGG TTAAGACCAT CGGCCTTAGG 
GAAGTGTGGT ACTTTGGCCT CCAGTATGTG GATAACAAAG GATTTCCTAC ATGGTTGAAA 
CTTGATAAAA AGGTGTCCGC CCAGGAGGTC AGGAAGGAGA GCCCGCTGCA GTTCAAGTTC 
CGGGCCAAGT TCTACCCCGA GGACGTGGCC GAGGAGCTCA TCCAGGACAT CACGCAGAAG 
CTCTTCTTCC TGCAAGTGAA GGAGGGCATC CTCAGCGACG AGATCTACTG CCCGCCCGAG 
ACCGCCGTGC TCCTGGGCTC CTACGCCGTG CAGGCCAAGT TCGGCGACTA CAACAAGGAG 
CTGCACAAGG CGGGGTACCT CGGCTCCGAG CGGCTCATCC CCCAGAGAGT CATGGACCAG 
CACAAGCTCA CCAGGGACCA GTGGGAGGAC CGGATCCAGG TGTGGCATGC AGAACACCGC 
GGGATGCTCA AGGACAGCGC CATGCTGGAA TACCTGAAGA TCGCCCAGGA CCTGGAAATG 
TACGGGATCA ACTATTTCGA GATAAAAAAT AAGAAAGGAA CAGACCTTTG GCTCGGCGTT 
GATGCCCTTG GACTAAATAT CTATGAGAAA GACGATAAGT TGACCCCAAA GATTGGCTTC 
CCATGGAGCG AGATCAGGAA CATCTCTTTC AATGACAAGA AGTTTGTCAT CAAGCCCATC 
GACAAGAAGG CTCCTGACTT CGTGTTCTAC GCCCCCCGCC TGCGGATCAA CAAGCGGATC 
CTGCAGCTCT GCATGGGGAA CCATGAGCTG TACATGCGCC GCCGGAAGCC CGACACCATT 
GAGGTGCAGC AGATGAAGGC CCAGGCCCGG GAGGAGAAGC ACCAGAAGCA GCTGGAGAGG 
CAGCAGTTGG AAACCGAGAA GAAGAGGAGA GAAACCGTGG AGCGAGAAAA AGAGCAGATG 
ATGCGTGAGA AGGAGGAGCT GATGCTCAGG CTTCAGGACT ACGAGGAAAA AACCAGGAAG 
GCTGAGAAAG AGCTCTCCGA CCAGATCCAG AGAGCGCTGA AGCTGGAGGA GGAGAGGAAG 
CGGGCCCAGG AGGAGGCGGG GCGCCTGGAG GCTGACCGCC TGGCCGCGCT GCGTGCCAAG 
GAGGAGCTGG AGAGGCAGGC AGCCGACCAG ATCAAGAGCC AGGAGCAGCT GGCCACAGAG 
CTCGCCGAGT ACACCGCCAA GATCGCCCTC CTGGAGGAGG CACGGCGGCG CAAGGAGAAT 
GAAGTGGAAG AGTGGCAGCT CCGGGCCAAG GAAGCCCAGG ATGACCTGGT GAAGACCCGG 
GAGGAGCTGC ACCTGGTGAT GACCGCGCCG CCGCCGCCAC CGGTGTACGA GCCGGTGAAC 
TACCACGTGC ATGAGGGCCC GCAGGAGGAG GGCACGGAGC TGAGCGCCGA GCTGTCCAGC 
GAGGGCATCC TGGACGACCG CAACGAGGAG AAGCGCATCA CCGAGGCCGA GAAGAACGAG 
CGCGTGCAGC GGCAGCTGAT GACTCTGACC AGTGAGCTGT CCCAGGCCAG AGACGAGAAC 
AAGCGGACCC ACAACGACAT CATCCACAAT GAGAACATGA GGCAAGGCCG GGACAAGTAC 
AAGACGCTGC GGCAGATCCG GCAAGGCAAC ACCAAGCAGC GCATCGACGA GTTCGAGGCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799028.1
CDS	195..1940
Translation	MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEVRKESPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKELHKAGYLGSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTRKAEKELSDQIQRALKLEEERKRAQEEAGRLEADRLAALRAKEELERQAADQIKSQEQLATELAEYTAKIALLEEARRRKENEVEEWQLRAKEAQDDLVKTREELHLVMTAPPPPPVYEPVNYHVHEGPQEEGTELSAELSSEGILDDRNEEKRITEAEKNERVQRQLMTLTSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAM

Target ORF information:

RefSeq Version	XM_010800726.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ezrin (EZR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800726.3

ORF Insert Sequence:

ATGCCGAAAC CGATCAACGT CCGAGTGACC ACCATGGATG CGGAGCTGGA GTTTGCCATC 
CAGCCAAACA CCACAGGAAA GCAGCTTTTT GATCAGGTGG TTAAGACCAT CGGCCTTAGG 
GAAGTGTGGT ACTTTGGCCT CCAGTATGTG GATAACAAAG GATTTCCTAC ATGGTTGAAA 
CTTGATAAAA AGGTGTCCGC CCAGGAGGTC AGGAAGGAGA GCCCGCTGCA GTTCAAGTTC 
CGGGCCAAGT TCTACCCCGA GGACGTGGCC GAGGAGCTCA TCCAGGACAT CACGCAGAAG 
CTCTTCTTCC TGCAAGTGAA GGAGGGCATC CTCAGCGACG AGATCTACTG CCCGCCCGAG 
ACCGCCGTGC TCCTGGGCTC CTACGCCGTG CAGGCCAAGT TCGGCGACTA CAACAAGGAG 
CTGCACAAGG CGGGGTACCT CGGCTCCGAG CGGCTCATCC CCCAGAGAGT CATGGACCAG 
CACAAGCTCA CCAGGGACCA GTGGGAGGAC CGGATCCAGG TGTGGCATGC AGAACACCGC 
GGGATGCTCA AGGACAGCGC CATGCTGGAA TACCTGAAGA TCGCCCAGGA CCTGGAAATG 
TACGGGATCA ACTATTTCGA GATAAAAAAT AAGAAAGGAA CAGACCTTTG GCTCGGCGTT 
GATGCCCTTG GACTAAATAT CTATGAGAAA GACGATAAGT TGACCCCAAA GATTGGCTTC 
CCATGGAGCG AGATCAGGAA CATCTCTTTC AATGACAAGA AGTTTGTCAT CAAGCCCATC 
GACAAGAAGG CTCCTGACTT CGTGTTCTAC GCCCCCCGCC TGCGGATCAA CAAGCGGATC 
CTGCAGCTCT GCATGGGGAA CCATGAGCTG TACATGCGCC GCCGGAAGCC CGACACCATT 
GAGGTGCAGC AGATGAAGGC CCAGGCCCGG GAGGAGAAGC ACCAGAAGCA GCTGGAGAGG 
CAGCAGTTGG AAACCGAGAA GAAGAGGAGA GAAACCGTGG AGCGAGAAAA AGAGCAGATG 
ATGCGTGAGA AGGAGGAGCT GATGCTCAGG CTTCAGGACT ACGAGGAAAA AACCAGGAAG 
GCTGAGAAAG AGCTCTCCGA CCAGATCCAG AGAGCGCTGA AGCTGGAGGA GGAGAGGAAG 
CGGGCCCAGG AGGAGGCGGG GCGCCTGGAG GCTGACCGCC TGGCCGCGCT GCGTGCCAAG 
GAGGAGCTGG AGAGGCAGGC AGCCGACCAG ATCAAGAGCC AGGAGCAGCT GGCCACAGAG 
CTCGCCGAGT ACACCGCCAA GATCGCCCTC CTGGAGGAGG CACGGCGGCG CAAGGAGAAT 
GAAGTGGAAG AGTGGCAGCT CCGGGCCAAG GAAGCCCAGG ATGACCTGGT GAAGACCCGG 
GAGGAGCTGC ACCTGGTGAT GACCGCGCCG CCGCCGCCAC CGGTGTACGA GCCGGTGAAC 
TACCACGTGC ATGAGGGCCC GCAGGAGGAG GGCACGGAGC TGAGCGCCGA GCTGTCCAGC 
GAGGGCATCC TGGACGACCG CAACGAGGAG AAGCGCATCA CCGAGGCCGA GAAGAACGAG 
CGCGTGCAGC GGCAGCTGAT GACTCTGACC AGTGAGCTGT CCCAGGCCAG AGACGAGAAC 
AAGCGGACCC ACAACGACAT CATCCACAAT GAGAACATGA GGCAAGGCCG GGACAAGTAC 
AAGACGCTGC GGCAGATCCG GCAAGGCAAC ACCAAGCAGC GCATCGACGA GTTCGAGGCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EZR ( XM_010800726.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799028.1 Bos taurus ezrin (EZR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa42177
Clone ID Related Accession (Same CDS sequence)	XM_010800726.3 , XM_010800726.2 , NM_174217.2
Accession Version	XM_010800726.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ezrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003069322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAAAC CGATCAACGT CCGAGTGACC ACCATGGATG CGGAGCTGGA GTTTGCCATC 
CAGCCAAACA CCACAGGAAA GCAGCTTTTT GATCAGGTGG TTAAGACCAT CGGCCTTAGG 
GAAGTGTGGT ACTTTGGCCT CCAGTATGTG GATAACAAAG GATTTCCTAC ATGGTTGAAA 
CTTGATAAAA AGGTGTCCGC CCAGGAGGTC AGGAAGGAGA GCCCGCTGCA GTTCAAGTTC 
CGGGCCAAGT TCTACCCCGA GGACGTGGCC GAGGAGCTCA TCCAGGACAT CACGCAGAAG 
CTCTTCTTCC TGCAAGTGAA GGAGGGCATC CTCAGCGACG AGATCTACTG CCCGCCCGAG 
ACCGCCGTGC TCCTGGGCTC CTACGCCGTG CAGGCCAAGT TCGGCGACTA CAACAAGGAG 
CTGCACAAGG CGGGGTACCT CGGCTCCGAG CGGCTCATCC CCCAGAGAGT CATGGACCAG 
CACAAGCTCA CCAGGGACCA GTGGGAGGAC CGGATCCAGG TGTGGCATGC AGAACACCGC 
GGGATGCTCA AGGACAGCGC CATGCTGGAA TACCTGAAGA TCGCCCAGGA CCTGGAAATG 
TACGGGATCA ACTATTTCGA GATAAAAAAT AAGAAAGGAA CAGACCTTTG GCTCGGCGTT 
GATGCCCTTG GACTAAATAT CTATGAGAAA GACGATAAGT TGACCCCAAA GATTGGCTTC 
CCATGGAGCG AGATCAGGAA CATCTCTTTC AATGACAAGA AGTTTGTCAT CAAGCCCATC 
GACAAGAAGG CTCCTGACTT CGTGTTCTAC GCCCCCCGCC TGCGGATCAA CAAGCGGATC 
CTGCAGCTCT GCATGGGGAA CCATGAGCTG TACATGCGCC GCCGGAAGCC CGACACCATT 
GAGGTGCAGC AGATGAAGGC CCAGGCCCGG GAGGAGAAGC ACCAGAAGCA GCTGGAGAGG 
CAGCAGTTGG AAACCGAGAA GAAGAGGAGA GAAACCGTGG AGCGAGAAAA AGAGCAGATG 
ATGCGTGAGA AGGAGGAGCT GATGCTCAGG CTTCAGGACT ACGAGGAAAA AACCAGGAAG 
GCTGAGAAAG AGCTCTCCGA CCAGATCCAG AGAGCGCTGA AGCTGGAGGA GGAGAGGAAG 
CGGGCCCAGG AGGAGGCGGG GCGCCTGGAG GCTGACCGCC TGGCCGCGCT GCGTGCCAAG 
GAGGAGCTGG AGAGGCAGGC AGCCGACCAG ATCAAGAGCC AGGAGCAGCT GGCCACAGAG 
CTCGCCGAGT ACACCGCCAA GATCGCCCTC CTGGAGGAGG CACGGCGGCG CAAGGAGAAT 
GAAGTGGAAG AGTGGCAGCT CCGGGCCAAG GAAGCCCAGG ATGACCTGGT GAAGACCCGG 
GAGGAGCTGC ACCTGGTGAT GACCGCGCCG CCGCCGCCAC CGGTGTACGA GCCGGTGAAC 
TACCACGTGC ATGAGGGCCC GCAGGAGGAG GGCACGGAGC TGAGCGCCGA GCTGTCCAGC 
GAGGGCATCC TGGACGACCG CAACGAGGAG AAGCGCATCA CCGAGGCCGA GAAGAACGAG 
CGCGTGCAGC GGCAGCTGAT GACTCTGACC AGTGAGCTGT CCCAGGCCAG AGACGAGAAC 
AAGCGGACCC ACAACGACAT CATCCACAAT GAGAACATGA GGCAAGGCCG GGACAAGTAC 
AAGACGCTGC GGCAGATCCG GCAAGGCAAC ACCAAGCAGC GCATCGACGA GTTCGAGGCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799028.1
CDS	192..1937
Translation	MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEVRKESPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKELHKAGYLGSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTRKAEKELSDQIQRALKLEEERKRAQEEAGRLEADRLAALRAKEELERQAADQIKSQEQLATELAEYTAKIALLEEARRRKENEVEEWQLRAKEAQDDLVKTREELHLVMTAPPPPPVYEPVNYHVHEGPQEEGTELSAELSSEGILDDRNEEKRITEAEKNERVQRQLMTLTSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAM

Target ORF information:

RefSeq Version	XM_010800726.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ezrin (EZR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010800726.2

ORF Insert Sequence:

ATGCCGAAAC CGATCAACGT CCGAGTGACC ACCATGGATG CGGAGCTGGA GTTTGCCATC 
CAGCCAAACA CCACAGGAAA GCAGCTTTTT GATCAGGTGG TTAAGACCAT CGGCCTTAGG 
GAAGTGTGGT ACTTTGGCCT CCAGTATGTG GATAACAAAG GATTTCCTAC ATGGTTGAAA 
CTTGATAAAA AGGTGTCCGC CCAGGAGGTC AGGAAGGAGA GCCCGCTGCA GTTCAAGTTC 
CGGGCCAAGT TCTACCCCGA GGACGTGGCC GAGGAGCTCA TCCAGGACAT CACGCAGAAG 
CTCTTCTTCC TGCAAGTGAA GGAGGGCATC CTCAGCGACG AGATCTACTG CCCGCCCGAG 
ACCGCCGTGC TCCTGGGCTC CTACGCCGTG CAGGCCAAGT TCGGCGACTA CAACAAGGAG 
CTGCACAAGG CGGGGTACCT CGGCTCCGAG CGGCTCATCC CCCAGAGAGT CATGGACCAG 
CACAAGCTCA CCAGGGACCA GTGGGAGGAC CGGATCCAGG TGTGGCATGC AGAACACCGC 
GGGATGCTCA AGGACAGCGC CATGCTGGAA TACCTGAAGA TCGCCCAGGA CCTGGAAATG 
TACGGGATCA ACTATTTCGA GATAAAAAAT AAGAAAGGAA CAGACCTTTG GCTCGGCGTT 
GATGCCCTTG GACTAAATAT CTATGAGAAA GACGATAAGT TGACCCCAAA GATTGGCTTC 
CCATGGAGCG AGATCAGGAA CATCTCTTTC AATGACAAGA AGTTTGTCAT CAAGCCCATC 
GACAAGAAGG CTCCTGACTT CGTGTTCTAC GCCCCCCGCC TGCGGATCAA CAAGCGGATC 
CTGCAGCTCT GCATGGGGAA CCATGAGCTG TACATGCGCC GCCGGAAGCC CGACACCATT 
GAGGTGCAGC AGATGAAGGC CCAGGCCCGG GAGGAGAAGC ACCAGAAGCA GCTGGAGAGG 
CAGCAGTTGG AAACCGAGAA GAAGAGGAGA GAAACCGTGG AGCGAGAAAA AGAGCAGATG 
ATGCGTGAGA AGGAGGAGCT GATGCTCAGG CTTCAGGACT ACGAGGAAAA AACCAGGAAG 
GCTGAGAAAG AGCTCTCCGA CCAGATCCAG AGAGCGCTGA AGCTGGAGGA GGAGAGGAAG 
CGGGCCCAGG AGGAGGCGGG GCGCCTGGAG GCTGACCGCC TGGCCGCGCT GCGTGCCAAG 
GAGGAGCTGG AGAGGCAGGC AGCCGACCAG ATCAAGAGCC AGGAGCAGCT GGCCACAGAG 
CTCGCCGAGT ACACCGCCAA GATCGCCCTC CTGGAGGAGG CACGGCGGCG CAAGGAGAAT 
GAAGTGGAAG AGTGGCAGCT CCGGGCCAAG GAAGCCCAGG ATGACCTGGT GAAGACCCGG 
GAGGAGCTGC ACCTGGTGAT GACCGCGCCG CCGCCGCCAC CGGTGTACGA GCCGGTGAAC 
TACCACGTGC ATGAGGGCCC GCAGGAGGAG GGCACGGAGC TGAGCGCCGA GCTGTCCAGC 
GAGGGCATCC TGGACGACCG CAACGAGGAG AAGCGCATCA CCGAGGCCGA GAAGAACGAG 
CGCGTGCAGC GGCAGCTGAT GACTCTGACC AGTGAGCTGT CCCAGGCCAG AGACGAGAAC 
AAGCGGACCC ACAACGACAT CATCCACAAT GAGAACATGA GGCAAGGCCG GGACAAGTAC 
AAGACGCTGC GGCAGATCCG GCAAGGCAAC ACCAAGCAGC GCATCGACGA GTTCGAGGCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sphingosine 1-phosphate activation of ERM contributes to vascular calcification.
Journal of lipid research59(1)69-78(2018 Jan)
Morris TG,Borland SJ,Clarke CJ,Wilson C,Hannun YA,Ohanian V,Canfield AE,Ohanian J

Changes in endometrial ezrin and cytokeratin 18 expression during bovine implantation and in caruncular endometrial spheroids in vitro.
Placenta36(8)821-31(2015 Aug)
Haeger JD,Hambruch N,Dantzer V,Hoelker M,Schellander K,Klisch K,Pfarrer C

An ezrin/calpain/PI3K/AMPK/eNOSs1179 signaling cascade mediating VEGF-dependent endothelial nitric oxide production.
Circulation research104(1)50-9(2009 Jan)
Youn JY,Wang T,Cai H

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Ezrin and osteonectin, two proteins associated with cell shape and growth, are enriched in the locus coeruleus.
Molecular and cellular neurosciences4(1)64-73(1993 Feb)
Bergson C,Zhao H,Saijoh K,Duman RS,Nestler EJ

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EZR cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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