VEGFA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	VEGFA
Entrez Gene ID	281572
Full Name	vascular endothelial growth factor A
Synonyms	VEGF,VEGF-A,VPF,eVEGF120,eVEGF164
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. It is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316993.1	NP_001303922.1	vascular endothelial growth factor A isoform 2 precursor
NM_001316956.1	NP_001303885.1	vascular endothelial growth factor A isoform 4 precursor
NM_001316955.1	NP_001303884.1	vascular endothelial growth factor A isoform VEGF-Ax precursor
NM_001316992.1	NP_001303921.1	vascular endothelial growth factor A isoform 3 precursor
NM_174216.2	NP_776641.1	vascular endothelial growth factor A isoform VEGF-A precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vegfa	NP_114024.2
Gallus gallus (chicken)	VEGFA	NP_990373.1
Canis lupus familiaris (dog)	VEGFA	NP_001003175.2
Bos taurus (cattle)	VEGFA	NP_776641.1
Mus musculus (house mouse)	Vegfa	NP_001020421.2
Homo sapiens (human)	VEGFA	NP_003367.4
Danio rerio (zebrafish)	vegfab	NP_001038320.2

Gene Ontology
Bibliography

Related articles in PubMed

Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING.
Zhang Y, Li Q, Youn JY, Cai H
The Journal of biological chemistry292(2)407-416(2017 Jan)

The interplay of cyclic stretch and vascular endothelial growth factor in regulating the initial steps for angiogenesis.
Wilkins JR, Pike DB, Gibson CC, Li L, Shiu YT
Biotechnology progress31(1)248-57(2015 Jan-Feb)

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Eswarappa SM, Potdar AA, Koch WJ, Fan Y, Vasu K, Lindner D, Willard B, Graham LM, DiCorleto PE, Fox PL
Cell157(7)1605-18(2014 Jun)

Diabetes-induced superoxide anion and breakdown of the blood-retinal barrier: role of the VEGF/uPAR pathway.
El-Remessy AB, Franklin T, Ghaley N, Yang J, Brands MW, Caldwell RB, Behzadian MA
PloS one8(8)e71868(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data for the first time demonstrate a calpain/PTP1B/VEGFR2 negative feedback loop in the regulation of VEGF-induced angiogenesis. Modulation of local PTP1B and/or calpain activities may prove beneficial in the treatment of impaired wound healing in diabetes.
Title: Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING.

Studied the effect of cyclic uniaxial stretch (20%, 1 Hz), with and without the stimulation of vascular endothelial growth factor (VEGF), on sprouting angiogenesis by employing a stretchable three-dimensional cell culture model.
Title: The interplay of cyclic stretch and vascular endothelial growth factor in regulating the initial steps for angiogenesis.

Study shows that VEGFA mRNA in mammalian endothelial cells undergoes programmed translational readthrough (PTR) generating VEGF-Ax, an isoform containing a unique 22-amino-acid C terminus extension.
Title: Programmed translational readthrough generates antiangiogenic VEGF-Ax.

Exposure of endothelial cells to VEGF, high glucose, or hydrogen peroxide up-regulated the XBP1/IRE1 alpha and ATF6 arms of the unfolded protein response compared with untreated cells.
Title: Targeting the IRE1?/XBP1 and ATF6 arms of the unfolded protein response enhances VEGF blockade to prevent retinal and choroidal neovascularization.

Data indicate that superoxide dismutase (SOD) inhibited high glucose (HG)-induced expression of uPAR and VEGF in bovine retinal microvascular endothelial cell (REC).
Title: Diabetes-induced superoxide anion and breakdown of the blood-retinal barrier: role of the VEGF/uPAR pathway.

The following VEGFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VEGFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01429	NM_174216.2 Latest version!	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa45168	NM_001316993.1 Latest version!	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa134049	NM_001316956.1 Latest version!	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OBa134050	NM_001316992.1 Latest version!	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OBa95409	NM_001316955.1 Latest version!	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VEGFA ( NM_174216.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776641.1 Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa01429
Clone ID Related Accession (Same CDS sequence)	NM_174216.2
Accession Version	NM_174216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Bos taurus(cattle)
Product	vascular endothelial growth factor A isoform VEGF-A precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, DV876964.1 and DV814183.1. On Nov 4, 2015 this sequence version replaced NM_174216.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (VEGF-A, also known as VEGF-A164) results from translation termination at the upstream UGA stop codon, while the longer isoform (VEGF-Ax) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (VEGF-A), which exhibits proangiogenic activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT 
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT 
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA 
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776641.1
CDS	1048..1620
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGQKPHEVVKFMDVYQRSFCRPIETLVDIFQEYPDEIEFIFKPSCVPLMRCGGCCNDESLECVPTEEFNITMQIMRIKPHQSQHIGEMSFLQHNKCECRPKKDKARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_174216.2
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174216.2

ORF Insert Sequence:

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT 
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT 
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA 
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001316993.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001303922.1 Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa45168
Clone ID Related Accession (Same CDS sequence)	NM_001316993.1
Accession Version	NM_001316993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Bos taurus(cattle)
Product	vascular endothelial growth factor A isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, AB455252.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1, and encodes a shorter isoform (2, also known as VEGF-A120) missing an internal protein segment compared to isoform VEGF-A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA 
TGTGACAAGC CGAGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303922.1
CDS	1048..1488
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGQKPHEVVKFMDVYQRSFCRPIETLVDIFQEYPDEIEFIFKPSCVPLMRCGGCCNDESLECVPTEEFNITMQIMRIKPHQSQHIGEMSFLQHNKCECRPKKDKARQEKCDKPRR

Target ORF information:

RefSeq Version	NM_001316993.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316993.1

ORF Insert Sequence:

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA 
TGTGACAAGC CGAGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001316956.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001303885.1 Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OBa134049
Clone ID Related Accession (Same CDS sequence)	NM_001316956.1
Accession Version	NM_001316956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Bos taurus(cattle)
Product	vascular endothelial growth factor A isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, AB450824.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (4) contains an additional in-frame coding exon compared to variant 1, and encodes a longer isoform (4) with an internal 24 aa protein segment missing in isoform VEGF-A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA 
AAATCAGTTC GAGGAAAGGG CAAAGGGCAA AAAAGAAAGC GCAAGAAATC CCGGTATAAA 
TCCTGGAGCG CTCCCTGTGG GCCTTGCTCA GAGCGGAGAA AGCATTTGTT TGTACAAGAT 
CCGCAGACGT GTAAATGTTC CTGCAAAAAC ACAGACTCGC GTTGCAAGGC GAGGCAGCTT 
GAGTTAAACG AACGTACTTG CAGATGTGAC AAGCCGAGGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303885.1
CDS	1048..1692
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGQKPHEVVKFMDVYQRSFCRPIETLVDIFQEYPDEIEFIFKPSCVPLMRCGGCCNDESLECVPTEEFNITMQIMRIKPHQSQHIGEMSFLQHNKCECRPKKDKARQEKKSVRGKGKGQKRKRKKSRYKSWSAPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Target ORF information:

RefSeq Version	NM_001316956.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316956.1

ORF Insert Sequence:

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAA 
AAATCAGTTC GAGGAAAGGG CAAAGGGCAA AAAAGAAAGC GCAAGAAATC CCGGTATAAA 
TCCTGGAGCG CTCCCTGTGG GCCTTGCTCA GAGCGGAGAA AGCATTTGTT TGTACAAGAT 
CCGCAGACGT GTAAATGTTC CTGCAAAAAC ACAGACTCGC GTTGCAAGGC GAGGCAGCTT 
GAGTTAAACG AACGTACTTG CAGATGTGAC AAGCCGAGGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001316992.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001303921.1 Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OBa134050
Clone ID Related Accession (Same CDS sequence)	NM_001316992.1
Accession Version	NM_001316992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Bos taurus(cattle)
Product	vascular endothelial growth factor A isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, EU017524.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (3) uses an alternate acceptor splice site at the 3' terminal exon, which causes a frameshift compared to variant 1. The encoded isoform (3, also known as VEGF-Ab), though the same length, has a distinct C-terminus compared to isoform VEGF-A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT 
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT 
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA 
CGTACTTGCA GACGTCTCAC CAGGAAAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303921.1
CDS	1048..1620
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGQKPHEVVKFMDVYQRSFCRPIETLVDIFQEYPDEIEFIFKPSCVPLMRCGGCCNDESLECVPTEEFNITMQIMRIKPHQSQHIGEMSFLQHNKCECRPKKDKARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRRLTRKD

Target ORF information:

RefSeq Version	NM_001316992.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316992.1

ORF Insert Sequence:

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT 
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT 
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA 
CGTACTTGCA GACGTCTCAC CAGGAAAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001316955.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001303884.1 Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa95409
Clone ID Related Accession (Same CDS sequence)	NM_001316955.1
Accession Version	NM_001316955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Bos taurus(cattle)
Product	vascular endothelial growth factor A isoform VEGF-Ax precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EV647554.1, HX921266.1, M32976.1, DV876964.1 and DV814183.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (VEGF-A, also known as VEGF-A164) results from translation termination at the upstream UGA stop codon, while the longer isoform (VEGF-Ax) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (VEGF-Ax), which exhibits antiangiogenic activity. As the upstream UGA stop codon has been shown to be recoded as serine (PMID:24949972), its location in the VEGF-Ax isoform (aa 191) is denoted by an 'S'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: PMID: 24949972 ##RefSeq-Attributes-END##

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT 
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT 
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA 
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGAGCCGGGC TGGAGGAAGG AGCCTCCCTC 
AGGGTTTCGG GAACCAGACG TCTCACCAGG AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303884.1
CDS	1048..1686
Translation	MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGQKPHEVVKFMDVYQRSFCRPIETLVDIFQEYPDEIEFIFKPSCVPLMRCGGCCNDESLECVPTEEFNITMQIMRIKPHQSQHIGEMSFLQHNKCECRPKKDKARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR*AGLEEGASLRVSGTRRLTRKD

Target ORF information:

RefSeq Version	NM_001316955.1
Organism	Bos taurus(cattle)
Definition	Bos taurus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316955.1

ORF Insert Sequence:

ATGAACTTTC TGCTCTCTTG GGTACATTGG AGCCTTGCCT TGCTGCTCTA CCTTCACCAT 
GCCAAGTGGT CCCAGGCTGC ACCCATGGCA GAAGGAGGGC AGAAACCCCA CGAAGTGGTG 
AAGTTCATGG ATGTCTACCA GCGCAGCTTC TGCCGTCCCA TCGAGACCCT GGTGGACATC 
TTCCAGGAGT ACCCAGATGA GATTGAGTTC ATTTTCAAGC CGTCCTGTGT GCCCCTGATG 
CGGTGCGGGG GCTGCTGTAA TGACGAAAGT CTGGAGTGTG TGCCCACTGA GGAGTTCAAC 
ATCACCATGC AGATTATGCG GATCAAACCT CACCAAAGCC AGCACATAGG AGAGATGAGC 
TTCCTACAGC ATAACAAATG TGAATGCAGA CCAAAGAAAG ATAAAGCAAG GCAAGAAAAT 
CCCTGTGGGC CTTGCTCAGA GCGGAGAAAG CATTTGTTTG TACAAGATCC GCAGACGTGT 
AAATGTTCCT GCAAAAACAC AGACTCGCGT TGCAAGGCGA GGCAGCTTGA GTTAAACGAA 
CGTACTTGCA GATGTGACAA GCCGAGGCGG TGAGCCGGGC TGGAGGAAGG AGCCTCCCTC 
AGGGTTTCGG GAACCAGACG TCTCACCAGG AAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING.
The Journal of biological chemistry292(2)407-416(2017 Jan)
Zhang Y,Li Q,Youn JY,Cai H

The interplay of cyclic stretch and vascular endothelial growth factor in regulating the initial steps for angiogenesis.
Biotechnology progress31(1)248-57(2015 Jan-Feb)
Wilkins JR,Pike DB,Gibson CC,Li L,Shiu YT

Programmed translational readthrough generates antiangiogenic VEGF-Ax.
Cell157(7)1605-18(2014 Jun)
Eswarappa SM,Potdar AA,Koch WJ,Fan Y,Vasu K,Lindner D,Willard B,Graham LM,DiCorleto PE,Fox PL

Diabetes-induced superoxide anion and breakdown of the blood-retinal barrier: role of the VEGF/uPAR pathway.
PloS one8(8)e71868(2013)
El-Remessy AB,Franklin T,Ghaley N,Yang J,Brands MW,Caldwell RB,Behzadian MA

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VEGFA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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