TSFM cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSFM
Entrez Gene ID	281551
Full Name	Ts translation elongation factor, mitochondrial
General protein information	Preferred Names Ts translation elongation factor, mitochondrial Names elongation factor Ts, mitochondrial EF-Ts EF-TsMt
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174204.3	NP_776629.1	elongation factor Ts, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSFM	XP_001167221.1
	TSFM	XP_001116504.2
Rattus norvegicus (Norway rat)	Tsfm	NP_001263405.1
Drosophila melanogaster (fruit fly)	CG6412	NP_609847.1
Caenorhabditis elegans (roundworm)	tsfm-1	NP_506079.1
Xenopus tropicalis (tropical clawed frog)	tsfm	XP_002933736.2
Mus musculus (house mouse)	Tsfm	NP_079813.1
Homo sapiens (human)	TSFM	NP_001166167.1
Bos taurus (cattle)	TSFM	NP_776629.1
Danio rerio (zebrafish)	tsfm	NP_001073504.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009234	XP_320011.4

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.
Akama K, Christian BE, Jones CN, Ueda T, Takeuchi N, Spremulli LL
Biochimica et biophysica acta1802(7-8)692-8(2010 Jul-Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.
Jeppesen MG, Navratil T, Spremulli LL, Nyborg J
The Journal of biological chemistry280(6)5071-81(2005 Feb)

Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes.
Karring H, Andersen GR, Thirup SS, Nyborg J, Spremulli LL, Clark BF
Biochimica et biophysica acta1601(2)172-7(2002 Dec)

Cloning and expression of mitochondrial translational elongation factor Ts from bovine and human liver.
Xin H, Woriax V, Burkhart W, Spremulli LL
The Journal of biological chemistry270(29)17243-9(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of activity is caused by a significant reduction in the ability of EF-Ts(mt) R325W to bind EF-Tu(mt), leading to a defect in nucleotide exchange.
Title: Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.

X-ray crystallographic structure of the EF-Tumt.Tsmt complex
Title: Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.

Isolation, crystallisation, and preliminary X-ray analysis
Title: Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes.

The following TSFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01284	NM_174204.3 Latest version!	Bos taurus Ts translation elongation factor, mitochondrial (TSFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSFM ( NM_174204.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776629.1 Bos taurus Ts translation elongation factor, mitochondrial (TSFM), mRNA.

Price & Availability↑

CloneID	OBa01284
Clone ID Related Accession (Same CDS sequence)	NM_174204.3
Accession Version	NM_174204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	elongation factor Ts, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L37935.1. On Feb 21, 2013 this sequence version replaced NM_174204.2. ##Evidence-Data-START## Transcript exon combination :: L37935.1, BC149988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCGCTGC TGCGGTCATT GCGTCTCTGC CTGGTCGCGC GGACCGGGAG CTGCCCGCTG 
AGCGCTCTTG GCCCTGGTCC CTTGCTGCCT TCCTTGCAGG CCGGGCTCCC TCTGCTTCAG 
TCGCCCCAGC AGTGGCATAC TTTTCACTCT GGATCCTGGC TGTCCTCGGC TTCCAGCAAG 
GAGCTTCTCA TGAAGCTGCG GAGGAAAACG GGCTACTCCT TTATAAACTG CAAGAAAGCC 
CTGGAGACTT GTGGCGGGGA TCTCAAACAG GCAGAGAGCT GGCTCCATAA GCAGGCCCAG 
AAGGAGGGCT GGAGTAAAGC TGCTAGGCTC CATGGGAGGA AGACCAAAGA AGGTCTGATT 
GGGCTGCTGC AGGAAGGAGA CACAACTGTG TTAGTAGAGG TGAACTGTGA GACAGATTTT 
GTTTCCAGAA ATTTAAAATT TCAACAGTTG GTCCAGCAAG TAGCCCTGGG AACCCTGTTG 
CATTGTCAGA ACTTAAAGGA TCAACTCTCC ACATACAGTA AAGGCTTCTT GAATTCCTCT 
GAGCTCTCTG AACTTCCAGC AGGACCTGAG AGAGAGGGTT CTCTCAAGGA TCAGCTGGCT 
TTAGCAATTG GGAAACTGGG AGAAAACATG ATTCTGAAAC GGGCTGCATG GGTGAAGGTG 
CCAGCTGGTT TCTATGTTGG CTCTTATGTC CATGGGGCAA TGCACAGCCC CTCACTCCAC 
AACCTGGTGC TGGGGAAGTA TGGGGCCCTG GTCATCTGCG AGACGTCAGA GTTGAAGGCA 
AACCTCGCAG ACCTTGGCCG CCGCCTTGGG CAGCACGTGG TAGGCATGGC TCCCCTCTCT 
GTTGGCTCCC TGGACGACGA GCCCGGGGGA GAGGCGGAAA CCAAGATGCT GTCCCAGCCA 
TACTTGCTGG ACCCTTCCAT CACACTGGGA CAGTACGTGC AGCCCCATGG GGTGTCCGTC 
GTAGACTTTG TGCGGTTTGA GTGTGGAGAA GGTGAAGACG CAGCAGACGC CGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776629.1
CDS	18..1034
Translation	MSLLRSLRLCLVARTGSCPLSALGPGPLLPSLQAGLPLLQSPQQWHTFHSGSWLSSASSKELLMKLRRKTGYSFINCKKALETCGGDLKQAESWLHKQAQKEGWSKAARLHGRKTKEGLIGLLQEGDTTVLVEVNCETDFVSRNLKFQQLVQQVALGTLLHCQNLKDQLSTYSKGFLNSSELSELPAGPEREGSLKDQLALAIGKLGENMILKRAAWVKVPAGFYVGSYVHGAMHSPSLHNLVLGKYGALVICETSELKANLADLGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPHGVSVVDFVRFECGEGEDAADAE

Target ORF information:

RefSeq Version	NM_174204.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Ts translation elongation factor, mitochondrial (TSFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174204.3

ORF Insert Sequence:

ATGTCGCTGC TGCGGTCATT GCGTCTCTGC CTGGTCGCGC GGACCGGGAG CTGCCCGCTG 
AGCGCTCTTG GCCCTGGTCC CTTGCTGCCT TCCTTGCAGG CCGGGCTCCC TCTGCTTCAG 
TCGCCCCAGC AGTGGCATAC TTTTCACTCT GGATCCTGGC TGTCCTCGGC TTCCAGCAAG 
GAGCTTCTCA TGAAGCTGCG GAGGAAAACG GGCTACTCCT TTATAAACTG CAAGAAAGCC 
CTGGAGACTT GTGGCGGGGA TCTCAAACAG GCAGAGAGCT GGCTCCATAA GCAGGCCCAG 
AAGGAGGGCT GGAGTAAAGC TGCTAGGCTC CATGGGAGGA AGACCAAAGA AGGTCTGATT 
GGGCTGCTGC AGGAAGGAGA CACAACTGTG TTAGTAGAGG TGAACTGTGA GACAGATTTT 
GTTTCCAGAA ATTTAAAATT TCAACAGTTG GTCCAGCAAG TAGCCCTGGG AACCCTGTTG 
CATTGTCAGA ACTTAAAGGA TCAACTCTCC ACATACAGTA AAGGCTTCTT GAATTCCTCT 
GAGCTCTCTG AACTTCCAGC AGGACCTGAG AGAGAGGGTT CTCTCAAGGA TCAGCTGGCT 
TTAGCAATTG GGAAACTGGG AGAAAACATG ATTCTGAAAC GGGCTGCATG GGTGAAGGTG 
CCAGCTGGTT TCTATGTTGG CTCTTATGTC CATGGGGCAA TGCACAGCCC CTCACTCCAC 
AACCTGGTGC TGGGGAAGTA TGGGGCCCTG GTCATCTGCG AGACGTCAGA GTTGAAGGCA 
AACCTCGCAG ACCTTGGCCG CCGCCTTGGG CAGCACGTGG TAGGCATGGC TCCCCTCTCT 
GTTGGCTCCC TGGACGACGA GCCCGGGGGA GAGGCGGAAA CCAAGATGCT GTCCCAGCCA 
TACTTGCTGG ACCCTTCCAT CACACTGGGA CAGTACGTGC AGCCCCATGG GGTGTCCGTC 
GTAGACTTTG TGCGGTTTGA GTGTGGAGAA GGTGAAGACG CAGCAGACGC CGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors.
Biochimica et biophysica acta1802(7-8)692-8(2010 Jul-Aug)
Akama K,Christian BE,Jones CN,Ueda T,Takeuchi N,Spremulli LL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.
The Journal of biological chemistry280(6)5071-81(2005 Feb)
Jeppesen MG,Navratil T,Spremulli LL,Nyborg J

Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes.
Biochimica et biophysica acta1601(2)172-7(2002 Dec)
Karring H,Andersen GR,Thirup SS,Nyborg J,Spremulli LL,Clark BF

Cloning and expression of mitochondrial translational elongation factor Ts from bovine and human liver.
The Journal of biological chemistry270(29)17243-9(1995 Jul)
Xin H,Woriax V,Burkhart W,Spremulli LL

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TSFM cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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