SPP1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPP1
Entrez Gene ID	281499
Full Name	secreted phosphoprotein 1
Synonyms	OPN,OST
General protein information	Preferred Names secreted phosphoprotein 1 Names osteopontin SPP-1 bone sialoprotein 1 bone sialoprotein I early T-lymphocyte activation 1 osteopontin-K secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005207742.2	XP_005207799.1	osteopontin isoform X3
XM_005207742.1	XP_005207799.1	osteopontin isoform X1
XM_024992984.1	XP_024848752.1	osteopontin isoform X2
XM_024992983.1	XP_024848751.1	osteopontin isoform X1
XM_024992985.1	XP_024848753.1	osteopontin isoform X3
XM_024992986.1	XP_024848754.1	osteopontin isoform X3
NM_174187.2	NP_776612.1	osteopontin precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPP1	XP_001159959.1
Canis lupus familiaris (dog)	SPP1	XP_003434072.1
Rattus norvegicus (Norway rat)	Spp1	NP_037013.2
	SPP1	XP_002804172.1
Mus musculus (house mouse)	Spp1	NP_001191130.1
Homo sapiens (human)	SPP1	NP_001035147.1
Bos taurus (cattle)	SPP1	NP_776612.1

Gene Ontology
Bibliography

Related articles in PubMed

Reactivity of U-associated osteopontin with lactoferrin: a one-to-many complex.
Ameziane-Le Hir S, Bourgeois D, Basset C, Hag?ge A, Vidaud C
Metallomics : integrated biometal science9(7)865-875(2017 Jul)

Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk.
Dudemaine PL, Thibault C, Alain K, Bissonnette N
Animal genetics45(5)629-40(2014 Oct)

Osteopontin binds multiple calcium ions with high affinity and independently of phosphorylation status.
Kl?ning E, Christensen B, S?rensen ES, Vorup-Jensen T, Jensen JK
Bone6690-5(2014 Sep)

Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
Santarelli A, Mascitti M, Orsini G, Mem? L, Rocchetti R, Tiriduzzi P, Sampalmieri F, Putignano A, Procaccini M, Lo Muzio L, Bambini F
Journal of biological regulators and homeostatic agents28(3)523-9(2014 Jul-Sep)

Characterization of UO2(2+) binding to osteopontin, a highly phosphorylated protein: insights into potential mechanisms of uranyl accumulation in bones.
Qi L, Basset C, Averseng O, Qu?m?neur E, Hag?ge A, Vidaud C
Metallomics : integrated biometal science6(1)166-76(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk.
Title: Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk.

This implies that a majority of the acidic residues within OPN must be engaged in calcium interaction under physiological conditions.
Title: Osteopontin binds multiple calcium ions with high affinity and independently of phosphorylation status.

Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
Title: Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.

osteopontin (OPN) can serve as a process-directing agent for the intrafibrillar mineralization of collagen.
Title: Multifunctional role of osteopontin in directing intrafibrillar mineralization of collagen and activation of osteoclasts.

Results suggest a mechanism of the interaction of UO2(2+) with bone metabolism and a new role for osteopontin (OPN) as a metal transporter.
Title: Characterization of UO2(2+) binding to osteopontin, a highly phosphorylated protein: insights into potential mechanisms of uranyl accumulation in bones.

The following SPP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01401	NM_174187.2 Latest version!	Bos taurus secreted phosphoprotein 1 (SPP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa20266	XM_024992985.1 Latest version!	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa20266	XM_005207742.2 Latest version!	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa20266	XM_024992986.1 Latest version!	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa235101	XM_024992983.1 Latest version!	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa235102	XM_024992984.1 Latest version!	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OBa20266	XM_005207742.1	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPP1 ( NM_174187.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776612.1 Bos taurus secreted phosphoprotein 1 (SPP1), mRNA.

Price & Availability↑

CloneID	OBa01401
Clone ID Related Accession (Same CDS sequence)	NM_174187.2
Accession Version	NM_174187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	osteopontin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M66236.1. On Jun 3, 2003 this sequence version replaced NM_174187.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M66236.1, BC102632.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145447, SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCATATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAACACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776612.1
CDS	64..900
Translation	MRIAVICFCLLGIASALPVKPTSSGSSEEKQLNNKYPDAVAIWLKPDPSQKQTFLTPQNSVSSEETDDNKQNTLPSKSNESPEQTDDLDDDDDNSQDVNSNDSDDAETTDDPDHSDESHHSDESDEVDFPTDIPTIAVFTPFIPTESANDGRGDSVAYGLKSRSKKFRRSNVQSPDATEEDFTSHIESEEMHDAPKKTSQLTDHSKETNSSELSKELTPKAKDKNKHSNLIESQENSKLSQEFHSLEDKLDLDHKSEEDKHLKIRISHELDSASSEVN

Target ORF information:

RefSeq Version	NM_174187.2
Organism	Bos taurus(cattle)
Definition	Bos taurus secreted phosphoprotein 1 (SPP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174187.2

ORF Insert Sequence:

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCATATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAACACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPP1 ( XM_005207742.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207799.1 Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa20266
Clone ID Related Accession (Same CDS sequence)	XM_005207742.2 , XM_005207742.1 , XM_024992985.1 , XM_024992986.1
Accession Version	XM_005207742.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	osteopontin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207799.1
CDS	380..1216
Translation	MRIAVICFCLLGIASALPVKPTSSGSSEEKQLNNKYPDAVATWLKPDPSQKQTFLAPQNSVSSEETDDNKQNTLPSKSNESPEQTDDLDDDDDNSQDVNSNDSDDAETTDDPDHSDESHHSDESDEVDFPTDIPTIAVFTPFIPTESANDGRGDSVAYGLKSRSKKFRRSNVQSPDATEEDFTSHIESEEMHDAPKKTSQLTDHSKETNSSELSKELTPKAKDKNKHSNLIESQENSKLSQEFHSLEDKLDLDHKSEEDKHLKIRISHELDSASSEVN

Target ORF information:

RefSeq Version	XM_005207742.2
Organism	Bos taurus(cattle)
Definition	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207742.2

ORF Insert Sequence:

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPP1 ( XM_005207742.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207799.1 Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20266
Clone ID Related Accession (Same CDS sequence)	XM_005207742.2 , XM_005207742.1 , XM_024992985.1 , XM_024992986.1
Accession Version	XM_005207742.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	osteopontin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103970.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207799.1
CDS	355..1191
Translation	MRIAVICFCLLGIASALPVKPTSSGSSEEKQLNNKYPDAVATWLKPDPSQKQTFLAPQNSVSSEETDDNKQNTLPSKSNESPEQTDDLDDDDDNSQDVNSNDSDDAETTDDPDHSDESHHSDESDEVDFPTDIPTIAVFTPFIPTESANDGRGDSVAYGLKSRSKKFRRSNVQSPDATEEDFTSHIESEEMHDAPKKTSQLTDHSKETNSSELSKELTPKAKDKNKHSNLIESQENSKLSQEFHSLEDKLDLDHKSEEDKHLKIRISHELDSASSEVN

Target ORF information:

RefSeq Version	XM_005207742.1
Organism	Bos taurus(cattle)
Definition	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207742.1

ORF Insert Sequence:

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPP1 ( XM_024992985.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848753.1 Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa20266
Clone ID Related Accession (Same CDS sequence)	XM_005207742.2 , XM_005207742.1 , XM_024992985.1 , XM_024992986.1
Accession Version	XM_024992985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	osteopontin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848753.1
CDS	572..1408
Translation	MRIAVICFCLLGIASALPVKPTSSGSSEEKQLNNKYPDAVATWLKPDPSQKQTFLAPQNSVSSEETDDNKQNTLPSKSNESPEQTDDLDDDDDNSQDVNSNDSDDAETTDDPDHSDESHHSDESDEVDFPTDIPTIAVFTPFIPTESANDGRGDSVAYGLKSRSKKFRRSNVQSPDATEEDFTSHIESEEMHDAPKKTSQLTDHSKETNSSELSKELTPKAKDKNKHSNLIESQENSKLSQEFHSLEDKLDLDHKSEEDKHLKIRISHELDSASSEVN

Target ORF information:

RefSeq Version	XM_024992985.1
Organism	Bos taurus(cattle)
Definition	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992985.1

ORF Insert Sequence:

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPP1 ( XM_024992986.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848754.1 Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa20266
Clone ID Related Accession (Same CDS sequence)	XM_005207742.2 , XM_005207742.1 , XM_024992985.1 , XM_024992986.1
Accession Version	XM_024992986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	osteopontin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848754.1
CDS	116..952
Translation	MRIAVICFCLLGIASALPVKPTSSGSSEEKQLNNKYPDAVATWLKPDPSQKQTFLAPQNSVSSEETDDNKQNTLPSKSNESPEQTDDLDDDDDNSQDVNSNDSDDAETTDDPDHSDESHHSDESDEVDFPTDIPTIAVFTPFIPTESANDGRGDSVAYGLKSRSKKFRRSNVQSPDATEEDFTSHIESEEMHDAPKKTSQLTDHSKETNSSELSKELTPKAKDKNKHSNLIESQENSKLSQEFHSLEDKLDLDHKSEEDKHLKIRISHELDSASSEVN

Target ORF information:

RefSeq Version	XM_024992986.1
Organism	Bos taurus(cattle)
Definition	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992986.1

ORF Insert Sequence:

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGCTTAACA ACAAATACCC AGATGCTGTA 
GCCACATGGC TAAAGCCTGA CCCATCTCAG AAGCAGACTT TCCTAGCACC ACAGAATTCT 
GTGTCCTCTG AGGAAACTGA TGACAACAAA CAAAATACCC TCCCAAGTAA GTCCAATGAA 
AGCCCTGAGC AAACAGACGA TCTAGATGAC GATGATGATA ACAGCCAGGA CGTCAACTCT 
AATGACTCCG ACGACGCTGA AACCACTGAT GACCCTGACC ATTCCGACGA GTCTCACCAT 
TCTGATGAAT CTGATGAAGT TGATTTTCCC ACTGATATTC CAACAATCGC AGTTTTCACT 
CCGTTTATCC CTACGGAAAG CGCAAATGAT GGCCGAGGTG ATAGTGTGGC TTACGGACTG 
AAGTCAAGAT CTAAGAAGTT CCGCCGATCT AACGTTCAGA GTCCAGATGC CACAGAGGAG 
GACTTCACAT CACACATAGA GAGTGAGGAG ATGCATGACG CACCTAAGAA GACGAGTCAG 
CTGACTGACC ACAGCAAGGA AACCAACAGT AGCGAGCTTT CCAAAGAACT CACGCCAAAG 
GCCAAGGATA AGAACAAGCA TTCCAATCTG ATTGAGAGTC AGGAAAATTC CAAACTCAGC 
CAAGAATTCC ATAGCCTTGA AGACAAGCTA GACCTAGATC ATAAGAGTGA AGAAGACAAA 
CACCTGAAAA TTCGTATTTC TCATGAATTA GATAGTGCCT CTTCTGAGGT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPP1 ( XM_024992983.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848751.1 Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235101
Clone ID Related Accession (Same CDS sequence)	XM_024992983.1
Accession Version	XM_024992983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	osteopontin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC ATGGTGGAGA GCATCTGGAG CAGCCTTTAA ATTCTGGGAG ATCCTGGTTG 
TCAGCAGCAG GGAGAGCAGG CCAGGAGGGC AGCACTGAGC ACTGCATCAG CATCACAGGG 
GACTGGACTC TTCTCGCCGC CGCAGACCAA GGAAAAATCA TTACCATGAG AATTGCAGTG 
ATTTGCTTCT GCCTCTTGGG CATTGCCTCC GCCCTTCCAG TTAAACCGAC CAGTTCTGGC 
AGCTCTGAGG AAAAGCAGCT TAACAACAAA TACCCAGATG CTGTAGCCAC ATGGCTAAAG 
CCTGACCCAT CTCAGAAGCA GACTTTCCTA GCACCACAGA ATTCTGTGTC CTCTGAGGAA 
ACTGATGACA ACAAACAAAA TACCCTCCCA AGTAAGTCCA ATGAAAGCCC TGAGCAAACA 
GACGATCTAG ATGACGATGA TGATAACAGC CAGGACGTCA ACTCTAATGA CTCCGACGAC 
GCTGAAACCA CTGATGACCC TGACCATTCC GACGAGTCTC ACCATTCTGA TGAATCTGAT 
GAAGTTGATT TTCCCACTGA TATTCCAACA ATCGCAGTTT TCACTCCGTT TATCCCTACG 
GAAAGCGCAA ATGATGGCCG AGGTGATAGT GTGGCTTACG GACTGAAGTC AAGATCTAAG 
AAGTTCCGCC GATCTAACGT TCAGAGTCCA GATGCCACAG AGGAGGACTT CACATCACAC 
ATAGAGAGTG AGGAGATGCA TGACGCACCT AAGAAGACGA GTCAGCTGAC TGACCACAGC 
AAGGAAACCA ACAGTAGCGA GCTTTCCAAA GAACTCACGC CAAAGGCCAA GGATAAGAAC 
AAGCATTCCA ATCTGATTGA GAGTCAGGAA AATTCCAAAC TCAGCCAAGA ATTCCATAGC 
CTTGAAGACA AGCTAGACCT AGATCATAAG AGTGAAGAAG ACAAACACCT GAAAATTCGT 
ATTTCTCATG AATTAGATAG TGCCTCTTCT GAGGTCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848751.1
CDS	293..1294
Translation	MERHGGEHLEQPLNSGRSWLSAAGRAGQEGSTEHCISITGDWTLLAAADQGKIITMRIAVICFCLLGIASALPVKPTSSGSSEEKQLNNKYPDAVATWLKPDPSQKQTFLAPQNSVSSEETDDNKQNTLPSKSNESPEQTDDLDDDDDNSQDVNSNDSDDAETTDDPDHSDESHHSDESDEVDFPTDIPTIAVFTPFIPTESANDGRGDSVAYGLKSRSKKFRRSNVQSPDATEEDFTSHIESEEMHDAPKKTSQLTDHSKETNSSELSKELTPKAKDKNKHSNLIESQENSKLSQEFHSLEDKLDLDHKSEEDKHLKIRISHELDSASSEVN

Target ORF information:

RefSeq Version	XM_024992983.1
Organism	Bos taurus(cattle)
Definition	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992983.1

ORF Insert Sequence:

ATGGAGAGGC ATGGTGGAGA GCATCTGGAG CAGCCTTTAA ATTCTGGGAG ATCCTGGTTG 
TCAGCAGCAG GGAGAGCAGG CCAGGAGGGC AGCACTGAGC ACTGCATCAG CATCACAGGG 
GACTGGACTC TTCTCGCCGC CGCAGACCAA GGAAAAATCA TTACCATGAG AATTGCAGTG 
ATTTGCTTCT GCCTCTTGGG CATTGCCTCC GCCCTTCCAG TTAAACCGAC CAGTTCTGGC 
AGCTCTGAGG AAAAGCAGCT TAACAACAAA TACCCAGATG CTGTAGCCAC ATGGCTAAAG 
CCTGACCCAT CTCAGAAGCA GACTTTCCTA GCACCACAGA ATTCTGTGTC CTCTGAGGAA 
ACTGATGACA ACAAACAAAA TACCCTCCCA AGTAAGTCCA ATGAAAGCCC TGAGCAAACA 
GACGATCTAG ATGACGATGA TGATAACAGC CAGGACGTCA ACTCTAATGA CTCCGACGAC 
GCTGAAACCA CTGATGACCC TGACCATTCC GACGAGTCTC ACCATTCTGA TGAATCTGAT 
GAAGTTGATT TTCCCACTGA TATTCCAACA ATCGCAGTTT TCACTCCGTT TATCCCTACG 
GAAAGCGCAA ATGATGGCCG AGGTGATAGT GTGGCTTACG GACTGAAGTC AAGATCTAAG 
AAGTTCCGCC GATCTAACGT TCAGAGTCCA GATGCCACAG AGGAGGACTT CACATCACAC 
ATAGAGAGTG AGGAGATGCA TGACGCACCT AAGAAGACGA GTCAGCTGAC TGACCACAGC 
AAGGAAACCA ACAGTAGCGA GCTTTCCAAA GAACTCACGC CAAAGGCCAA GGATAAGAAC 
AAGCATTCCA ATCTGATTGA GAGTCAGGAA AATTCCAAAC TCAGCCAAGA ATTCCATAGC 
CTTGAAGACA AGCTAGACCT AGATCATAAG AGTGAAGAAG ACAAACACCT GAAAATTCGT 
ATTTCTCATG AATTAGATAG TGCCTCTTCT GAGGTCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPP1 ( XM_024992984.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848752.1 Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235102
Clone ID Related Accession (Same CDS sequence)	XM_024992984.1
Accession Version	XM_024992984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	osteopontin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGAATTCTG TGTCCTCTGA GGAAACTGAT 
GACAACAAAC AAAATACCCT CCCAAGTAAG TCCAATGAAA GCCCTGAGCA AACAGACGAT 
CTAGATGACG ATGATGATAA CAGCCAGGAC GTCAACTCTA ATGACTCCGA CGACGCTGAA 
ACCACTGATG ACCCTGACCA TTCCGACGAG TCTCACCATT CTGATGAATC TGATGAAGTT 
GATTTTCCCA CTGATATTCC AACAATCGCA GTTTTCACTC CGTTTATCCC TACGGAAAGC 
GCAAATGATG GCCGAGGTGA TAGTGTGGCT TACGGACTGA AGTCAAGATC TAAGAAGTTC 
CGCCGATCTA ACGTTCAGAG TCCAGATGCC ACAGAGGAGG ACTTCACATC ACACATAGAG 
AGTGAGGAGA TGCATGACGC ACCTAAGAAG ACGAGTCAGC TGACTGACCA CAGCAAGGAA 
ACCAACAGTA GCGAGCTTTC CAAAGAACTC ACGCCAAAGG CCAAGGATAA GAACAAGCAT 
TCCAATCTGA TTGAGAGTCA GGAAAATTCC AAACTCAGCC AAGAATTCCA TAGCCTTGAA 
GACAAGCTAG ACCTAGATCA TAAGAGTGAA GAAGACAAAC ACCTGAAAAT TCGTATTTCT 
CATGAATTAG ATAGTGCCTC TTCTGAGGTC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848752.1
CDS	153..908
Translation	MRIAVICFCLLGIASALPVKPTSSGSSEEKQNSVSSEETDDNKQNTLPSKSNESPEQTDDLDDDDDNSQDVNSNDSDDAETTDDPDHSDESHHSDESDEVDFPTDIPTIAVFTPFIPTESANDGRGDSVAYGLKSRSKKFRRSNVQSPDATEEDFTSHIESEEMHDAPKKTSQLTDHSKETNSSELSKELTPKAKDKNKHSNLIESQENSKLSQEFHSLEDKLDLDHKSEEDKHLKIRISHELDSASSEVN

Target ORF information:

RefSeq Version	XM_024992984.1
Organism	Bos taurus(cattle)
Definition	Bos taurus secreted phosphoprotein 1 (SPP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992984.1

ORF Insert Sequence:

ATGAGAATTG CAGTGATTTG CTTCTGCCTC TTGGGCATTG CCTCCGCCCT TCCAGTTAAA 
CCGACCAGTT CTGGCAGCTC TGAGGAAAAG CAGAATTCTG TGTCCTCTGA GGAAACTGAT 
GACAACAAAC AAAATACCCT CCCAAGTAAG TCCAATGAAA GCCCTGAGCA AACAGACGAT 
CTAGATGACG ATGATGATAA CAGCCAGGAC GTCAACTCTA ATGACTCCGA CGACGCTGAA 
ACCACTGATG ACCCTGACCA TTCCGACGAG TCTCACCATT CTGATGAATC TGATGAAGTT 
GATTTTCCCA CTGATATTCC AACAATCGCA GTTTTCACTC CGTTTATCCC TACGGAAAGC 
GCAAATGATG GCCGAGGTGA TAGTGTGGCT TACGGACTGA AGTCAAGATC TAAGAAGTTC 
CGCCGATCTA ACGTTCAGAG TCCAGATGCC ACAGAGGAGG ACTTCACATC ACACATAGAG 
AGTGAGGAGA TGCATGACGC ACCTAAGAAG ACGAGTCAGC TGACTGACCA CAGCAAGGAA 
ACCAACAGTA GCGAGCTTTC CAAAGAACTC ACGCCAAAGG CCAAGGATAA GAACAAGCAT 
TCCAATCTGA TTGAGAGTCA GGAAAATTCC AAACTCAGCC AAGAATTCCA TAGCCTTGAA 
GACAAGCTAG ACCTAGATCA TAAGAGTGAA GAAGACAAAC ACCTGAAAAT TCGTATTTCT 
CATGAATTAG ATAGTGCCTC TTCTGAGGTC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reactivity of U-associated osteopontin with lactoferrin: a one-to-many complex.
Metallomics : integrated biometal science9(7)865-875(2017 Jul)
Ameziane-Le Hir S,Bourgeois D,Basset C,Hag?ge A,Vidaud C

Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk.
Animal genetics45(5)629-40(2014 Oct)
Dudemaine PL,Thibault C,Alain K,Bissonnette N

Osteopontin binds multiple calcium ions with high affinity and independently of phosphorylation status.
Bone6690-5(2014 Sep)
Kl?ning E,Christensen B,S?rensen ES,Vorup-Jensen T,Jensen JK

Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
Journal of biological regulators and homeostatic agents28(3)523-9(2014 Jul-Sep)
Santarelli A,Mascitti M,Orsini G,Mem? L,Rocchetti R,Tiriduzzi P,Sampalmieri F,Putignano A,Procaccini M,Lo Muzio L,Bambini F

Characterization of UO2(2+) binding to osteopontin, a highly phosphorylated protein: insights into potential mechanisms of uranyl accumulation in bones.
Metallomics : integrated biometal science6(1)166-76(2014 Jan)
Qi L,Basset C,Averseng O,Qu?m?neur E,Hag?ge A,Vidaud C

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SPP1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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