RCVRN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RCVRN
Entrez Gene ID	281447
Full Name	recoverin
Synonyms	RCV1
General protein information	Preferred Names recoverin Names recoverin p26
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174165.2	NP_776590.1	recoverin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RCVRN	XP_511841.2
Rattus norvegicus (Norway rat)	Rcvrn	NP_543177.1
Danio rerio (zebrafish)	rcvrna	NP_001025419.1
Homo sapiens (human)	RCVRN	NP_002894.1
	RCVRN	XP_001113548.1
Mus musculus (house mouse)	Rcvrn	NP_033064.1
Danio rerio (zebrafish)	LOC792903	XP_001332545.4
Canis lupus familiaris (dog)	RCVRN	NP_001014303.1
Bos taurus (cattle)	RCVRN	NP_776590.1
Xenopus tropicalis (tropical clawed frog)	rcvrn.1	XP_002937040.1

Gene Ontology
Bibliography

Related articles in PubMed

Conformational dynamics of recoverin's Ca2+-myristoyl switch probed by 15N NMR relaxation dispersion and chemical shift analysis.
Xu X, Ishima R, Ames JB
Proteins79(6)1910-22(2011 Jun)

Involvement of the recoverin C-terminal segment in recognition of the target enzyme rhodopsin kinase.
Zernii EY, Komolov KE, Permyakov SE, Kolpakova T, Dell'orco D, Poetzsch A, Knyazeva EL, Grigoriev II, Permyakov EA, Senin II, Philippov PP, Koch KW
The Biochemical journal435(2)441-50(2011 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Membrane binding of the neuronal calcium sensor recoverin - modulatory role of the charged carboxy-terminus.
Senin II, Churumova VA, Philippov PP, Koch KW
BMC biochemistry824(2007 Nov)

Ca2+-dependent conformational changes in the neuronal Ca2+-sensor recoverin probed by the fluorescent dye Alexa647.
Gensch T, Komolov KE, Senin II, Philippov PP, Koch KW
Proteins66(2)492-9(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

(15) N NMR relaxation data suggest that Ca(2+)-free recoverin undergoes millisecond conformational dynamics at particular amide sites throughout the protein.
Title: Conformational dynamics of recoverin's Ca2+-myristoyl switch probed by 15N NMR relaxation dispersion and chemical shift analysis.

A novel rhodopsin-kinase-binding site within the C-terminal region of recoverin.
Title: Involvement of the recoverin C-terminal segment in recognition of the target enzyme rhodopsin kinase.

Properties of the charged carboxy-terminus are consistent with a role of this region as an internal effector region that prolongs the time recoverin stays on the membrane by influencing its Ca2+-sensitivity
Title: Membrane binding of the neuronal calcium sensor recoverin - modulatory role of the charged carboxy-terminus.

Time-resolved fluorescence shows biexponential fluorescence decay. The slower time constant was affected by Ca2+-induced conformational changes in WT & RecE85Q (disabled EF-hand 2), & not RecE121Q (EF-hand 3 nonfunctional) with a clamped myristoyl group.
Title: Ca2+-dependent conformational changes in the neuronal Ca2+-sensor recoverin probed by the fluorescent dye Alexa647.

propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity
Title: Tuning of a neuronal calcium sensor.

The following RCVRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RCVRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43823	NM_174165.2 Latest version!	Bos taurus recoverin (RCVRN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RCVRN ( NM_174165.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776590.1 Bos taurus recoverin (RCVRN), mRNA.

Price & Availability↑

CloneID	OBa43823
Clone ID Related Accession (Same CDS sequence)	NM_174165.2
Accession Version	NM_174165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	recoverin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X63322.1. On Jun 3, 2003 this sequence version replaced NM_174165.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X63322.1, M95858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145474 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAACA GCAAGAGTGG GGCCCTGTCC AAGGAGATCC TGGAGGAGCT GCAGCTGAAC 
ACCAAGTTCA CGGAGGAGGA GCTGAGCTCC TGGTACCAGT CCTTCCTGAA GGAGTGCCCC 
AGTGGCCGGA TCACCCGGCA GGAGTTCCAG ACCATCTACT CCAAGTTCTT CCCCGAGGCC 
GACCCCAAGG CCTATGCCCA GCACGTGTTC CGAAGCTTTG ATGCCAACAG CGATGGCACC 
TTGGACTTCA AGGAGTATGT CATCGCCCTA CACATGACCA GCGCGGGCAA GACCAACCAG 
AAGCTGGAGT GGGCCTTCTC CCTCTATGAT GTGGATGGCA ATGGGACCAT CAGCAAGAAC 
GAGGTGCTGG AGATTGTCAC GGCTATCTTC AAAATGATCA GCCCTGAGGA CACAAAGCAT 
CTCCCAGAAG ACGAGAACAC TCCGGAAAAG CGAGCAGAGA AGATCTGGGG ATTCTTTGGC 
AAGAAGGATG ATGATAAACT TACAGAGAAA GAATTCATCG AAGGGACCCT GGCCAATAAG 
GAAATTCTGC GACTGATTCA ATTCGAGCCT CAAAAAGTGA AGGAGAAACT GAAGGAAAAG 
AAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776590.1
CDS	62..670
Translation	MGNSKSGALSKEILEELQLNTKFTEEELSSWYQSFLKECPSGRITRQEFQTIYSKFFPEADPKAYAQHVFRSFDANSDGTLDFKEYVIALHMTSAGKTNQKLEWAFSLYDVDGNGTISKNEVLEIVTAIFKMISPEDTKHLPEDENTPEKRAEKIWGFFGKKDDDKLTEKEFIEGTLANKEILRLIQFEPQKVKEKLKEKKL

Target ORF information:

RefSeq Version	NM_174165.2
Organism	Bos taurus(cattle)
Definition	Bos taurus recoverin (RCVRN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174165.2

ORF Insert Sequence:

ATGGGGAACA GCAAGAGTGG GGCCCTGTCC AAGGAGATCC TGGAGGAGCT GCAGCTGAAC 
ACCAAGTTCA CGGAGGAGGA GCTGAGCTCC TGGTACCAGT CCTTCCTGAA GGAGTGCCCC 
AGTGGCCGGA TCACCCGGCA GGAGTTCCAG ACCATCTACT CCAAGTTCTT CCCCGAGGCC 
GACCCCAAGG CCTATGCCCA GCACGTGTTC CGAAGCTTTG ATGCCAACAG CGATGGCACC 
TTGGACTTCA AGGAGTATGT CATCGCCCTA CACATGACCA GCGCGGGCAA GACCAACCAG 
AAGCTGGAGT GGGCCTTCTC CCTCTATGAT GTGGATGGCA ATGGGACCAT CAGCAAGAAC 
GAGGTGCTGG AGATTGTCAC GGCTATCTTC AAAATGATCA GCCCTGAGGA CACAAAGCAT 
CTCCCAGAAG ACGAGAACAC TCCGGAAAAG CGAGCAGAGA AGATCTGGGG ATTCTTTGGC 
AAGAAGGATG ATGATAAACT TACAGAGAAA GAATTCATCG AAGGGACCCT GGCCAATAAG 
GAAATTCTGC GACTGATTCA ATTCGAGCCT CAAAAAGTGA AGGAGAAACT GAAGGAAAAG 
AAACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conformational dynamics of recoverin's Ca2+-myristoyl switch probed by 15N NMR relaxation dispersion and chemical shift analysis.
Proteins79(6)1910-22(2011 Jun)
Xu X,Ishima R,Ames JB

Involvement of the recoverin C-terminal segment in recognition of the target enzyme rhodopsin kinase.
The Biochemical journal435(2)441-50(2011 Apr)
Zernii EY,Komolov KE,Permyakov SE,Kolpakova T,Dell'orco D,Poetzsch A,Knyazeva EL,Grigoriev II,Permyakov EA,Senin II,Philippov PP,Koch KW

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Membrane binding of the neuronal calcium sensor recoverin - modulatory role of the charged carboxy-terminus.
BMC biochemistry824(2007 Nov)
Senin II,Churumova VA,Philippov PP,Koch KW

Ca2+-dependent conformational changes in the neuronal Ca2+-sensor recoverin probed by the fluorescent dye Alexa647.
Proteins66(2)492-9(2007 Feb)
Gensch T,Komolov KE,Senin II,Philippov PP,Koch KW

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RCVRN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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