DAG1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005222705.3	XP_005222762.1	dystroglycan isoform X2
XM_015459535.1	XP_015315021.1	dystroglycan isoform X2
XM_005222707.4	XP_005222764.2	dystroglycan isoform X2
XM_010817663.2	XP_010815965.2	dystroglycan isoform X1
XM_024982387.1	XP_024838155.1	dystroglycan isoform X1
XM_005222705.4	XP_005222762.2	dystroglycan isoform X2
NM_174162.1	NP_776587.1	dystroglycan precursor
XM_005222706.3	XP_005222763.1	dystroglycan isoform X2
XM_005222707.3	XP_005222764.1	dystroglycan isoform X2
XM_005222706.4	XP_005222763.2	dystroglycan isoform X2
XM_015459535.2	XP_015315021.2	dystroglycan isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41459	NM_174162.1 Latest version!	Bos taurus dystroglycan 1 (DAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa31486	XM_005222707.3 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa31486	XM_015459535.1 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa31486	XM_005222706.3 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa31486	XM_005222705.3 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa228727	XM_005222705.4 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228727	XM_005222707.4 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228727	XM_005222706.4 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228727	XM_015459535.2 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa228726	XM_024982387.1 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa94399	XM_010817663.2 Latest version!	Bos taurus dystroglycan 1 (DAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

DAG1 ( NM_174162.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776587.1 Bos taurus dystroglycan 1 (DAG1), mRNA.

CloneID	OBa41459
Clone ID Related Accession (Same CDS sequence)	NM_174162.1
Accession Version	NM_174162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	dystroglycan precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB009079.1. ##Evidence-Data-START## Transcript exon combination :: AB009079.1, CN793926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAGGATGT CTGTGGGCTC CGCGGTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCTGCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC AACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCT CAACAGTCAC CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCAACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGGC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCCCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776587.1
CDS	1..2688
Translation	MRMSVGSAVPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCCLNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPSTVTTPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPATRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	NM_174162.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174162.1

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC CGCGGTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCTGCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC AACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCT CAACAGTCAC CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCAACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGGC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCCCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_005222705.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222762.1 Bos taurus dystroglycan 1 (DAG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa31486
Clone ID Related Accession (Same CDS sequence)	XM_005222705.3 , XM_015459535.1 , XM_005222706.3 , XM_005222707.3
Accession Version	XM_005222705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222762.1
CDS	259..2946
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005222705.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222705.3

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_015459535.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315021.1 Bos taurus dystroglycan 1 (DAG1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa31486
Clone ID Related Accession (Same CDS sequence)	XM_005222705.3 , XM_015459535.1 , XM_005222706.3 , XM_005222707.3
Accession Version	XM_015459535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315021.1
CDS	168..2855
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_015459535.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459535.1

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_005222706.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222763.1 Bos taurus dystroglycan 1 (DAG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa31486
Clone ID Related Accession (Same CDS sequence)	XM_005222705.3 , XM_015459535.1 , XM_005222706.3 , XM_005222707.3
Accession Version	XM_005222706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222763.1
CDS	250..2937
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005222706.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222706.3

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_005222707.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222764.1 Bos taurus dystroglycan 1 (DAG1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa31486
Clone ID Related Accession (Same CDS sequence)	XM_005222705.3 , XM_015459535.1 , XM_005222706.3 , XM_005222707.3
Accession Version	XM_005222707.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222707.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222764.1
CDS	217..2904
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005222707.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222707.3

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTATCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATTATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCACT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCGTGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_005222707.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222764.2 Bos taurus dystroglycan 1 (DAG1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa228727
Clone ID Related Accession (Same CDS sequence)	XM_005222707.4 , XM_005222705.4 , XM_005222706.4 , XM_015459535.2
Accession Version	XM_005222707.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222707.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222764.2
CDS	237..2924
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005222707.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222707.4

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_005222705.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222762.2 Bos taurus dystroglycan 1 (DAG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa228727
Clone ID Related Accession (Same CDS sequence)	XM_005222707.4 , XM_005222705.4 , XM_005222706.4 , XM_015459535.2
Accession Version	XM_005222705.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222705.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222762.2
CDS	293..2980
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005222705.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222705.4

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_005222706.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222763.2 Bos taurus dystroglycan 1 (DAG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa228727
Clone ID Related Accession (Same CDS sequence)	XM_005222707.4 , XM_005222705.4 , XM_005222706.4 , XM_015459535.2
Accession Version	XM_005222706.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222706.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222763.2
CDS	569..3256
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_005222706.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222706.4

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_015459535.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315021.2 Bos taurus dystroglycan 1 (DAG1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa228727
Clone ID Related Accession (Same CDS sequence)	XM_005222707.4 , XM_005222705.4 , XM_005222706.4 , XM_015459535.2
Accession Version	XM_015459535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315021.2
CDS	168..2855
Translation	MRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_015459535.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459535.2

ORF Insert Sequence:

ATGAGGATGT CTGTGGGCTC GTCGCTGCCG CTTCCCCTCT GGGGGAGGAC CTTTCTCCTT 
CTGTTGTCTG TGGCCGTGAC TCAGTCCCAC TGGCCCAGTG AACCTTCGGA GGCCGTCAGG 
GACTGGGAGA ACCAGCTCGA GGCGTCCATG CACTCAGCAC TCTCAGACCT CCACGAGACT 
GTTCCCACCG TGGTTGGCAT TCCTGATGGC ACGGCTGTCG TCGGACGCTC ATTTCGAGTG 
ACCATTCCAA CAGATTTAAT TGCTTCCAAT GGAGAAGTCA TCAAGGTATC AGCCGCGGGG 
AAGGAGGCCT TACCATCCTG GCTGCACTGG GACCCGCAGA GCCACACCCT GGAGGGTCTC 
CCCCTTGACA CCGATAAGGG GGTGCATTAC ATTTCAGTCA GCGCTGCACG ACTGGGGGCC 
AATGGGAGCC ACGTCCCCCA GACTTCCAGC GTGTTCTCTA TCGAGGTGTA CCCTGAAGAC 
CACAGCGAGC CGCAGTCGCT GCGGGCGGCC TCACCGGACC CCGGCGAGGT GGTGTCCTTG 
GTCTGTGCTG CTGATGAGCC TGTGACCGTC CTGACAGTCA TTCTGGACGC TGACCTCACC 
AAGATGACCC CAAAGCAAAG GATCGACCTG CTGCGCCGGA TGCGGGGCTT CTCTGAAGTT 
GAGCCGCACA ACATGAAGCT GGTACCCGTG GTGAACAACA GACTGTTTGA CATGTCGGCT 
TTCATGGCAG GCCCAGGCAA TGCAAAGAAG GTGGTGGAGA ACGGGGCCCT GCTCTCCTGG 
AAGCTGGGCT GCGCCCTGAA CCAGAACAGT GTGCCTGACA TTCGTGGTGT GGAGGTCCCC 
GCCAGGGAGG GCGCCATGTC TGCGCAGCTC GGCTACCCTG TGGTGGGCTG GCACATCGCC 
AACAAGAAAC CCTCTCTGCC CAAACGCATC CGGAGGCAGA TCCATGCCAC GCCCACGCCT 
GTCACGGCCA TTGGGCCCCC CACCACGGCC ATCCAGGAGC CACCGTCCAG AATTGTGCCC 
ACCCCCACGT CTCCAGCCAT TGCTCCTCCA ACCGAGACCA TGGCTCCTCC GGTCAGGGAC 
CCTGTTCCCG GGAAGCCCAC AGTCACCATT CGGACTCGAG GTGCCATTAT TCAGACCCCA 
ACCCTTGGCC CCATCCAGCC CACTCGGGTG TCAGAAGCTG GCACCACAGT TCCCAGCCAG 
ATCCGCCCAA CAATGACCAT TCCTGGTTAC ATGGAGCCCA CAGCAGTCAT CACCCCTCCT 
ACGACGACCA CCAAGAAGCC ACGAGTGTCC ACGCCCAGAC CAGCCACACC TTCTACTGAC 
TCCTCTACCA CCACTACTCG AAGGCCAACC AAGAAGCCGC GGACATCCCG ACCGGTGCCC 
CGGGTCACCA CCAAGGCACC CATCACCAGA CTGGAAACCG CCTCCCCGCC GACACGCATG 
CGCACCACCA CCAGCGGGGT ACCCCATGGA GGGGAACCCA ACCAGCGTCC AGAGCTCAAG 
AACCACATCG ACAGGGTGGA CGCCTGGGTC GGCACCTACT TTGAGGTGAA GATCCCATCG 
GATACCTTCT ACGACAATGA GGACACCACC ACTGATAAGC TGAAGTTGAC CCTGAAGCTT 
CGGGAGCAGC AGCTGGTAGG CGAGAAGTCG TGGGTGCAGT TCAACAGCAA CAGCCAGCTC 
ATGTATGGCC TGCCAGACAG CAGCCACGTG GGCAAGCACG AGTACTTCAT GCACGCCACG 
GACAAGGGGG GCCTGTCGGC GGTGGACGCC TTTGAGATTC ATGTCCACAG GCGCCCGCAA 
GGGGACAAGG CTCCTGCACG CTTCAAGGCT AAGTTGACAG GTGACCCTGC AGCCGTGACG 
AACGACATTC ACAAGAAGAT TGCCCTGGTG AAGAAGCTGG CCTTTGCCTT CGGGGACCGC 
AACTGCAGTA CCATCACCCT GCAGAACATC ACCCGGGGCT CCATTGTGGT GGAGTGGACC 
AACAATACCC TGCCCCTGGA GCCCTGCCCC AAGGAGCAGA TCACGGCGCT GAGCCGGAGG 
ATCGCTGAGG ATGACGGGAA GCCTCGAGGC GCCTTCGTCA ACGCACTGGA GCCTGACTTC 
CAGGCCATGA GCATCACAGT GACGGGCTCT GGCAGCTGCC GGCATCTCCA GTTTGTCCCA 
GTGGCACCGC CCATGAGGGT GCCCTCGGAG GCACCAGCCA CGGAGGTGCC AGACCGGGAC 
CCCGAGAAGA GCAGCGAGGA CGATGTGTAC TTGCACACGG TCATCCCGGC CGTGGTGGTG 
GCGGCCATCC TGCTCATCGC GGGCATCATT GCCATGATCT GCTACCGCAA GAAGCGGAAG 
GGCAAGCTCA CCCTGGAGGA CCAAGCCACC TTCATCAAGA AGGGGGTGCC CATCATCTTT 
GCGGACGAGC TGGACGACTC CAAGCCCCCG CCCTCCTCCA GCATGCCGCT GATCCTGCAG 
GAGGAGAAAG CCCCCCTCCC CCCTCCCGAG TACCCCAACC AGAGCATGCC CGAGACCACT 
CCCCTGAACC AGGACACCGT GGGAGAGTAT GCGCCCCTGC GGGATGAGGA TCCCAGCGCG 
CCGCCCTACC AGCCCCCGCC GCCCTTCACA GCCCCCATGG AGGGCAAGGG CTCCCGGCCC 
AAGAACATGA CCCCCTACCG GTCGCCCCCT CCCTACGTGC CCCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_024982387.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838155.1 Bos taurus dystroglycan 1 (DAG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228726
Clone ID Related Accession (Same CDS sequence)	XM_024982387.1
Accession Version	XM_024982387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTGGC TGGCCGAGCG GGTGTTCAGA GTGCCAGGGG CTTCCCCCGG GCTCTGGGAA 
GGCCCTCTTC ACAGAGGGGC TTTGCTGGGG TCTGGAGCAG TGCTCAGCTC AGACTCGATG 
GTGGACATTG CACTAGGGGC TGGCACAGAG GAGGACGTTA CCCAGAGCCA CTACCCACGT 
CTCAGCAGGA GAGCTTCGGC AGCCTCCATC AGCCACACGG TGGCTCTGGG TGCTCTGGGA 
TGGAGCAGGT GTGCAGAGGG TGAGGACCCA GTCCTGGGAC CACGTTGCTT GCTTCTTTTC 
TTGGAGAGAC TTATCTGCCT GAACAGACTT GAACCGGGGA TGAGGATGTC TGTGGGCTCG 
TCGCTGCCGC TTCCCCTCTG GGGGAGGACC TTTCTCCTTC TGTTGTCTGT GGCCGTGACT 
CAGTCCCACT GGCCCAGTGA ACCTTCGGAG GCCGTCAGGG ACTGGGAGAA CCAGCTCGAG 
GCGTCCATGC ACTCAGCACT CTCAGACCTC CACGAGACTG TTCCCACCGT GGTTGGCATT 
CCTGATGGCA CGGCTGTCGT CGGACGCTCA TTTCGAGTGA CCATTCCAAC AGATTTAATT 
GCTTCCAATG GAGAAGTCAT CAAGGTATCA GCCGCGGGGA AGGAGGCCTT ACCATCCTGG 
CTGCACTGGG ACCCGCAGAG CCACACCCTG GAGGGTCTCC CCCTTGACAC CGATAAGGGG 
GTGCATTACA TTTCAGTCAG CGCTGCACGA CTGGGGGCCA ATGGGAGCCA CGTCCCCCAG 
ACTTCCAGCG TGTTCTCTAT CGAGGTGTAC CCTGAAGACC ACAGCGAGCC GCAGTCGCTG 
CGGGCGGCCT CACCGGACCC CGGCGAGGTG GTGTCCTTGG TCTGTGCTGC TGATGAGCCT 
GTGACCGTCC TGACAGTCAT TCTGGACGCT GACCTCACCA AGATGACCCC AAAGCAAAGG 
ATCGACCTGC TGCGCCGGAT GCGGGGCTTC TCTGAAGTTG AGCCGCACAA CATGAAGCTG 
GTACCCGTGG TGAACAACAG ACTGTTTGAC ATGTCGGCTT TCATGGCAGG CCCAGGCAAT 
GCAAAGAAGG TGGTGGAGAA CGGGGCCCTG CTCTCCTGGA AGCTGGGCTG CGCCCTGAAC 
CAGAACAGTG TGCCTGACAT TCGTGGTGTG GAGGTCCCCG CCAGGGAGGG CGCCATGTCT 
GCGCAGCTCG GCTACCCTGT GGTGGGCTGG CACATCGCCA ACAAGAAACC CTCTCTGCCC 
AAACGCATCC GGAGGCAGAT CCATGCCACG CCCACGCCTG TCACGGCCAT TGGGCCCCCC 
ACCACGGCCA TCCAGGAGCC ACCGTCCAGA ATTGTGCCCA CCCCCACGTC TCCAGCCATT 
GCTCCTCCAA CCGAGACCAT GGCTCCTCCG GTCAGGGACC CTGTTCCCGG GAAGCCCACA 
GTCACCATTC GGACTCGAGG TGCCATTATT CAGACCCCAA CCCTTGGCCC CATCCAGCCC 
ACTCGGGTGT CAGAAGCTGG CACCACAGTT CCCAGCCAGA TCCGCCCAAC AATGACCATT 
CCTGGTTACA TGGAGCCCAC AGCAGTCATC ACCCCTCCTA CGACGACCAC CAAGAAGCCA 
CGAGTGTCCA CGCCCAGACC AGCCACACCT TCTACTGACT CCTCTACCAC CACTACTCGA 
AGGCCAACCA AGAAGCCGCG GACATCCCGA CCGGTGCCCC GGGTCACCAC CAAGGCACCC 
ATCACCAGAC TGGAAACCGC CTCCCCGCCG ACACGCATGC GCACCACCAC CAGCGGGGTA 
CCCCATGGAG GGGAACCCAA CCAGCGTCCA GAGCTCAAGA ACCACATCGA CAGGGTGGAC 
GCCTGGGTCG GCACCTACTT TGAGGTGAAG ATCCCATCGG ATACCTTCTA CGACAATGAG 
GACACCACCA CTGATAAGCT GAAGTTGACC CTGAAGCTTC GGGAGCAGCA GCTGGTAGGC 
GAGAAGTCGT GGGTGCAGTT CAACAGCAAC AGCCAGCTCA TGTATGGCCT GCCAGACAGC 
AGCCACGTGG GCAAGCACGA GTACTTCATG CACGCCACGG ACAAGGGGGG CCTGTCGGCG 
GTGGACGCCT TTGAGATTCA TGTCCACAGG CGCCCGCAAG GGGACAAGGC TCCTGCACGC 
TTCAAGGCTA AGTTGACAGG TGACCCTGCA GCCGTGACGA ACGACATTCA CAAGAAGATT 
GCCCTGGTGA AGAAGCTGGC CTTTGCCTTC GGGGACCGCA ACTGCAGTAC CATCACCCTG 
CAGAACATCA CCCGGGGCTC CATTGTGGTG GAGTGGACCA ACAATACCCT GCCCCTGGAG 
CCCTGCCCCA AGGAGCAGAT CACGGCGCTG AGCCGGAGGA TCGCTGAGGA TGACGGGAAG 
CCTCGAGGCG CCTTCGTCAA CGCACTGGAG CCTGACTTCC AGGCCATGAG CATCACAGTG 
ACGGGCTCTG GCAGCTGCCG GCATCTCCAG TTTGTCCCAG TGGCACCGCC CATGAGGGTG 
CCCTCGGAGG CACCAGCCAC GGAGGTGCCA GACCGGGACC CCGAGAAGAG CAGCGAGGAC 
GATGTGTACT TGCACACGGT CATCCCGGCC GTGGTGGTGG CGGCCATCCT GCTCATCGCG 
GGCATCATTG CCATGATCTG CTACCGCAAG AAGCGGAAGG GCAAGCTCAC CCTGGAGGAC 
CAAGCCACCT TCATCAAGAA GGGGGTGCCC ATCATCTTTG CGGACGAGCT GGACGACTCC 
AAGCCCCCGC CCTCCTCCAG CATGCCGCTG ATCCTGCAGG AGGAGAAAGC CCCCCTCCCC 
CCTCCCGAGT ACCCCAACCA GAGCATGCCC GAGACCACTC CCCTGAACCA GGACACCGTG 
GGAGAGTATG CGCCCCTGCG GGATGAGGAT CCCAGCGCGC CGCCCTACCA GCCCCCGCCG 
CCCTTCACAG CCCCCATGGA GGGCAAGGGC TCCCGGCCCA AGAACATGAC CCCCTACCGG 
TCGCCCCCTC CCTACGTGCC CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838155.1
CDS	192..3218
Translation	MPWLAERVFRVPGASPGLWEGPLHRGALLGSGAVLSSDSMVDIALGAGTEEDVTQSHYPRLSRRASAASISHTVALGALGWSRCAEGEDPVLGPRCLLLFLERLICLNRLEPGMRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_024982387.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982387.1

ORF Insert Sequence:

ATGCCGTGGC TGGCCGAGCG GGTGTTCAGA GTGCCAGGGG CTTCCCCCGG GCTCTGGGAA 
GGCCCTCTTC ACAGAGGGGC TTTGCTGGGG TCTGGAGCAG TGCTCAGCTC AGACTCGATG 
GTGGACATTG CACTAGGGGC TGGCACAGAG GAGGACGTTA CCCAGAGCCA CTACCCACGT 
CTCAGCAGGA GAGCTTCGGC AGCCTCCATC AGCCACACGG TGGCTCTGGG TGCTCTGGGA 
TGGAGCAGGT GTGCAGAGGG TGAGGACCCA GTCCTGGGAC CACGTTGCTT GCTTCTTTTC 
TTGGAGAGAC TTATCTGCCT GAACAGACTT GAACCGGGGA TGAGGATGTC TGTGGGCTCG 
TCGCTGCCGC TTCCCCTCTG GGGGAGGACC TTTCTCCTTC TGTTGTCTGT GGCCGTGACT 
CAGTCCCACT GGCCCAGTGA ACCTTCGGAG GCCGTCAGGG ACTGGGAGAA CCAGCTCGAG 
GCGTCCATGC ACTCAGCACT CTCAGACCTC CACGAGACTG TTCCCACCGT GGTTGGCATT 
CCTGATGGCA CGGCTGTCGT CGGACGCTCA TTTCGAGTGA CCATTCCAAC AGATTTAATT 
GCTTCCAATG GAGAAGTCAT CAAGGTATCA GCCGCGGGGA AGGAGGCCTT ACCATCCTGG 
CTGCACTGGG ACCCGCAGAG CCACACCCTG GAGGGTCTCC CCCTTGACAC CGATAAGGGG 
GTGCATTACA TTTCAGTCAG CGCTGCACGA CTGGGGGCCA ATGGGAGCCA CGTCCCCCAG 
ACTTCCAGCG TGTTCTCTAT CGAGGTGTAC CCTGAAGACC ACAGCGAGCC GCAGTCGCTG 
CGGGCGGCCT CACCGGACCC CGGCGAGGTG GTGTCCTTGG TCTGTGCTGC TGATGAGCCT 
GTGACCGTCC TGACAGTCAT TCTGGACGCT GACCTCACCA AGATGACCCC AAAGCAAAGG 
ATCGACCTGC TGCGCCGGAT GCGGGGCTTC TCTGAAGTTG AGCCGCACAA CATGAAGCTG 
GTACCCGTGG TGAACAACAG ACTGTTTGAC ATGTCGGCTT TCATGGCAGG CCCAGGCAAT 
GCAAAGAAGG TGGTGGAGAA CGGGGCCCTG CTCTCCTGGA AGCTGGGCTG CGCCCTGAAC 
CAGAACAGTG TGCCTGACAT TCGTGGTGTG GAGGTCCCCG CCAGGGAGGG CGCCATGTCT 
GCGCAGCTCG GCTACCCTGT GGTGGGCTGG CACATCGCCA ACAAGAAACC CTCTCTGCCC 
AAACGCATCC GGAGGCAGAT CCATGCCACG CCCACGCCTG TCACGGCCAT TGGGCCCCCC 
ACCACGGCCA TCCAGGAGCC ACCGTCCAGA ATTGTGCCCA CCCCCACGTC TCCAGCCATT 
GCTCCTCCAA CCGAGACCAT GGCTCCTCCG GTCAGGGACC CTGTTCCCGG GAAGCCCACA 
GTCACCATTC GGACTCGAGG TGCCATTATT CAGACCCCAA CCCTTGGCCC CATCCAGCCC 
ACTCGGGTGT CAGAAGCTGG CACCACAGTT CCCAGCCAGA TCCGCCCAAC AATGACCATT 
CCTGGTTACA TGGAGCCCAC AGCAGTCATC ACCCCTCCTA CGACGACCAC CAAGAAGCCA 
CGAGTGTCCA CGCCCAGACC AGCCACACCT TCTACTGACT CCTCTACCAC CACTACTCGA 
AGGCCAACCA AGAAGCCGCG GACATCCCGA CCGGTGCCCC GGGTCACCAC CAAGGCACCC 
ATCACCAGAC TGGAAACCGC CTCCCCGCCG ACACGCATGC GCACCACCAC CAGCGGGGTA 
CCCCATGGAG GGGAACCCAA CCAGCGTCCA GAGCTCAAGA ACCACATCGA CAGGGTGGAC 
GCCTGGGTCG GCACCTACTT TGAGGTGAAG ATCCCATCGG ATACCTTCTA CGACAATGAG 
GACACCACCA CTGATAAGCT GAAGTTGACC CTGAAGCTTC GGGAGCAGCA GCTGGTAGGC 
GAGAAGTCGT GGGTGCAGTT CAACAGCAAC AGCCAGCTCA TGTATGGCCT GCCAGACAGC 
AGCCACGTGG GCAAGCACGA GTACTTCATG CACGCCACGG ACAAGGGGGG CCTGTCGGCG 
GTGGACGCCT TTGAGATTCA TGTCCACAGG CGCCCGCAAG GGGACAAGGC TCCTGCACGC 
TTCAAGGCTA AGTTGACAGG TGACCCTGCA GCCGTGACGA ACGACATTCA CAAGAAGATT 
GCCCTGGTGA AGAAGCTGGC CTTTGCCTTC GGGGACCGCA ACTGCAGTAC CATCACCCTG 
CAGAACATCA CCCGGGGCTC CATTGTGGTG GAGTGGACCA ACAATACCCT GCCCCTGGAG 
CCCTGCCCCA AGGAGCAGAT CACGGCGCTG AGCCGGAGGA TCGCTGAGGA TGACGGGAAG 
CCTCGAGGCG CCTTCGTCAA CGCACTGGAG CCTGACTTCC AGGCCATGAG CATCACAGTG 
ACGGGCTCTG GCAGCTGCCG GCATCTCCAG TTTGTCCCAG TGGCACCGCC CATGAGGGTG 
CCCTCGGAGG CACCAGCCAC GGAGGTGCCA GACCGGGACC CCGAGAAGAG CAGCGAGGAC 
GATGTGTACT TGCACACGGT CATCCCGGCC GTGGTGGTGG CGGCCATCCT GCTCATCGCG 
GGCATCATTG CCATGATCTG CTACCGCAAG AAGCGGAAGG GCAAGCTCAC CCTGGAGGAC 
CAAGCCACCT TCATCAAGAA GGGGGTGCCC ATCATCTTTG CGGACGAGCT GGACGACTCC 
AAGCCCCCGC CCTCCTCCAG CATGCCGCTG ATCCTGCAGG AGGAGAAAGC CCCCCTCCCC 
CCTCCCGAGT ACCCCAACCA GAGCATGCCC GAGACCACTC CCCTGAACCA GGACACCGTG 
GGAGAGTATG CGCCCCTGCG GGATGAGGAT CCCAGCGCGC CGCCCTACCA GCCCCCGCCG 
CCCTTCACAG CCCCCATGGA GGGCAAGGGC TCCCGGCCCA AGAACATGAC CCCCTACCGG 
TCGCCCCCTC CCTACGTGCC CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_010817663.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815965.2 Bos taurus dystroglycan 1 (DAG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa94399
Clone ID Related Accession (Same CDS sequence)	XM_010817663.2
Accession Version	XM_010817663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	dystroglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104544.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTGGC TGGCCGAGCG GGTGTTCAGA GTGCCAGGGG CTTCCCCCGG GCTCTGGGAA 
GGCCCTCTTC ACAGAGGGGC TTTGCTGGGG TCTGGAGCAG TGCTCAGCTC AGACTCGATG 
GTGGACATTG CACTAGGGGC TGGCACAGAG GAGGACGTTA CCCAGAGCCA CTACCCACGT 
CTCAGCAGGA GAGCTTCGGC AGCCTCCATC AGCCACACGG TGGCTCTGGG TGCTCTGGGA 
TGGAGCAGGT GTGCAGAGGG TGAGGACCCA GTCCTGGGAC CACGTTGCTT GCTTCTTTTC 
TTGGAGAGAC TTATCTGCCT GAACAGACTT GAACCGGGGA TGAGGATGTC TGTGGGCTCG 
TCGCTGCCGC TTCCCCTCTG GGGGAGGACC TTTCTCCTTC TGTTGTCTGT GGCCGTGACT 
CAGTCCCACT GGCCCAGTGA ACCTTCGGAG GCCGTCAGGG ACTGGGAGAA CCAGCTCGAG 
GCGTCCATGC ACTCAGCACT CTCAGACCTC CACGAGACTG TTCCCACCGT GGTTGGCATT 
CCTGATGGCA CGGCTGTCGT CGGACGCTCA TTTCGAGTGA CCATTCCAAC AGATTTAATT 
GCTTCCAATG GAGAAGTCAT CAAGGTATCA GCCGCGGGGA AGGAGGCCTT ACCATCCTGG 
CTGCACTGGG ACCCGCAGAG CCACACCCTG GAGGGTCTCC CCCTTGACAC CGATAAGGGG 
GTGCATTACA TTTCAGTCAG CGCTGCACGA CTGGGGGCCA ATGGGAGCCA CGTCCCCCAG 
ACTTCCAGCG TGTTCTCTAT CGAGGTGTAC CCTGAAGACC ACAGCGAGCC GCAGTCGCTG 
CGGGCGGCCT CACCGGACCC CGGCGAGGTG GTGTCCTTGG TCTGTGCTGC TGATGAGCCT 
GTGACCGTCC TGACAGTCAT TCTGGACGCT GACCTCACCA AGATGACCCC AAAGCAAAGG 
ATCGACCTGC TGCGCCGGAT GCGGGGCTTC TCTGAAGTTG AGCCGCACAA CATGAAGCTG 
GTACCCGTGG TGAACAACAG ACTGTTTGAC ATGTCGGCTT TCATGGCAGG CCCAGGCAAT 
GCAAAGAAGG TGGTGGAGAA CGGGGCCCTG CTCTCCTGGA AGCTGGGCTG CGCCCTGAAC 
CAGAACAGTG TGCCTGACAT TCGTGGTGTG GAGGTCCCCG CCAGGGAGGG CGCCATGTCT 
GCGCAGCTCG GCTACCCTGT GGTGGGCTGG CACATCGCCA ACAAGAAACC CTCTCTGCCC 
AAACGCATCC GGAGGCAGAT CCATGCCACG CCCACGCCTG TCACGGCCAT TGGGCCCCCC 
ACCACGGCCA TCCAGGAGCC ACCGTCCAGA ATTGTGCCCA CCCCCACGTC TCCAGCCATT 
GCTCCTCCAA CCGAGACCAT GGCTCCTCCG GTCAGGGACC CTGTTCCCGG GAAGCCCACA 
GTCACCATTC GGACTCGAGG TGCCATTATT CAGACCCCAA CCCTTGGCCC CATCCAGCCC 
ACTCGGGTGT CAGAAGCTGG CACCACAGTT CCCAGCCAGA TCCGCCCAAC AATGACCATT 
CCTGGTTACA TGGAGCCCAC AGCAGTCATC ACCCCTCCTA CGACGACCAC CAAGAAGCCA 
CGAGTGTCCA CGCCCAGACC AGCCACACCT TCTACTGACT CCTCTACCAC CACTACTCGA 
AGGCCAACCA AGAAGCCGCG GACATCCCGA CCGGTGCCCC GGGTCACCAC CAAGGCACCC 
ATCACCAGAC TGGAAACCGC CTCCCCGCCG ACACGCATGC GCACCACCAC CAGCGGGGTA 
CCCCATGGAG GGGAACCCAA CCAGCGTCCA GAGCTCAAGA ACCACATCGA CAGGGTGGAC 
GCCTGGGTCG GCACCTACTT TGAGGTGAAG ATCCCATCGG ATACCTTCTA CGACAATGAG 
GACACCACCA CTGATAAGCT GAAGTTGACC CTGAAGCTTC GGGAGCAGCA GCTGGTAGGC 
GAGAAGTCGT GGGTGCAGTT CAACAGCAAC AGCCAGCTCA TGTATGGCCT GCCAGACAGC 
AGCCACGTGG GCAAGCACGA GTACTTCATG CACGCCACGG ACAAGGGGGG CCTGTCGGCG 
GTGGACGCCT TTGAGATTCA TGTCCACAGG CGCCCGCAAG GGGACAAGGC TCCTGCACGC 
TTCAAGGCTA AGTTGACAGG TGACCCTGCA GCCGTGACGA ACGACATTCA CAAGAAGATT 
GCCCTGGTGA AGAAGCTGGC CTTTGCCTTC GGGGACCGCA ACTGCAGTAC CATCACCCTG 
CAGAACATCA CCCGGGGCTC CATTGTGGTG GAGTGGACCA ACAATACCCT GCCCCTGGAG 
CCCTGCCCCA AGGAGCAGAT CACGGCGCTG AGCCGGAGGA TCGCTGAGGA TGACGGGAAG 
CCTCGAGGCG CCTTCGTCAA CGCACTGGAG CCTGACTTCC AGGCCATGAG CATCACAGTG 
ACGGGCTCTG GCAGCTGCCG GCATCTCCAG TTTGTCCCAG TGGCACCGCC CATGAGGGTG 
CCCTCGGAGG CACCAGCCAC GGAGGTGCCA GACCGGGACC CCGAGAAGAG CAGCGAGGAC 
GATGTGTACT TGCACACGGT CATCCCGGCC GTGGTGGTGG CGGCCATCCT GCTCATCGCG 
GGCATCATTG CCATGATCTG CTATCGCAAG AAGCGGAAGG GCAAGCTCAC CCTGGAGGAC 
CAAGCCACCT TCATCAAGAA GGGGGTGCCC ATTATCTTTG CGGACGAGCT GGACGACTCC 
AAGCCCCCGC CCTCCTCCAG CATGCCACTG ATCCTGCAGG AGGAGAAAGC CCCCCTCCCC 
CCTCCCGAGT ACCCCAACCA GAGCGTGCCC GAGACCACTC CCCTGAACCA GGACACCGTG 
GGAGAGTATG CGCCCCTGCG GGATGAGGAT CCCAGCGCGC CGCCCTACCA GCCCCCGCCG 
CCCTTCACAG CCCCCATGGA GGGCAAGGGC TCCCGGCCCA AGAACATGAC CCCCTACCGG 
TCGCCCCCTC CCTACGTGCC CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815965.2
CDS	1500..4526
Translation	MPWLAERVFRVPGASPGLWEGPLHRGALLGSGAVLSSDSMVDIALGAGTEEDVTQSHYPRLSRRASAASISHTVALGALGWSRCAEGEDPVLGPRCLLLFLERLICLNRLEPGMRMSVGSSLPLPLWGRTFLLLLSVAVTQSHWPSEPSEAVRDWENQLEASMHSALSDLHETVPTVVGIPDGTAVVGRSFRVTIPTDLIASNGEVIKVSAAGKEALPSWLHWDPQSHTLEGLPLDTDKGVHYISVSAARLGANGSHVPQTSSVFSIEVYPEDHSEPQSLRAASPDPGEVVSLVCAADEPVTVLTVILDADLTKMTPKQRIDLLRRMRGFSEVEPHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCALNQNSVPDIRGVEVPAREGAMSAQLGYPVVGWHIANKKPSLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPSQIRPTMTIPGYMEPTAVITPPTTTTKKPRVSTPRPATPSTDSSTTTTRRPTKKPRTSRPVPRVTTKAPITRLETASPPTRMRTTTSGVPHGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDKAPARFKAKLTGDPAAVTNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQITALSRRIAEDDGKPRGAFVNALEPDFQAMSITVTGSGSCRHLQFVPVAPPMRVPSEAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAMICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPLNQDTVGEYAPLRDEDPSAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_010817663.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dystroglycan 1 (DAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817663.2

ORF Insert Sequence:

ATGCCGTGGC TGGCCGAGCG GGTGTTCAGA GTGCCAGGGG CTTCCCCCGG GCTCTGGGAA 
GGCCCTCTTC ACAGAGGGGC TTTGCTGGGG TCTGGAGCAG TGCTCAGCTC AGACTCGATG 
GTGGACATTG CACTAGGGGC TGGCACAGAG GAGGACGTTA CCCAGAGCCA CTACCCACGT 
CTCAGCAGGA GAGCTTCGGC AGCCTCCATC AGCCACACGG TGGCTCTGGG TGCTCTGGGA 
TGGAGCAGGT GTGCAGAGGG TGAGGACCCA GTCCTGGGAC CACGTTGCTT GCTTCTTTTC 
TTGGAGAGAC TTATCTGCCT GAACAGACTT GAACCGGGGA TGAGGATGTC TGTGGGCTCG 
TCGCTGCCGC TTCCCCTCTG GGGGAGGACC TTTCTCCTTC TGTTGTCTGT GGCCGTGACT 
CAGTCCCACT GGCCCAGTGA ACCTTCGGAG GCCGTCAGGG ACTGGGAGAA CCAGCTCGAG 
GCGTCCATGC ACTCAGCACT CTCAGACCTC CACGAGACTG TTCCCACCGT GGTTGGCATT 
CCTGATGGCA CGGCTGTCGT CGGACGCTCA TTTCGAGTGA CCATTCCAAC AGATTTAATT 
GCTTCCAATG GAGAAGTCAT CAAGGTATCA GCCGCGGGGA AGGAGGCCTT ACCATCCTGG 
CTGCACTGGG ACCCGCAGAG CCACACCCTG GAGGGTCTCC CCCTTGACAC CGATAAGGGG 
GTGCATTACA TTTCAGTCAG CGCTGCACGA CTGGGGGCCA ATGGGAGCCA CGTCCCCCAG 
ACTTCCAGCG TGTTCTCTAT CGAGGTGTAC CCTGAAGACC ACAGCGAGCC GCAGTCGCTG 
CGGGCGGCCT CACCGGACCC CGGCGAGGTG GTGTCCTTGG TCTGTGCTGC TGATGAGCCT 
GTGACCGTCC TGACAGTCAT TCTGGACGCT GACCTCACCA AGATGACCCC AAAGCAAAGG 
ATCGACCTGC TGCGCCGGAT GCGGGGCTTC TCTGAAGTTG AGCCGCACAA CATGAAGCTG 
GTACCCGTGG TGAACAACAG ACTGTTTGAC ATGTCGGCTT TCATGGCAGG CCCAGGCAAT 
GCAAAGAAGG TGGTGGAGAA CGGGGCCCTG CTCTCCTGGA AGCTGGGCTG CGCCCTGAAC 
CAGAACAGTG TGCCTGACAT TCGTGGTGTG GAGGTCCCCG CCAGGGAGGG CGCCATGTCT 
GCGCAGCTCG GCTACCCTGT GGTGGGCTGG CACATCGCCA ACAAGAAACC CTCTCTGCCC 
AAACGCATCC GGAGGCAGAT CCATGCCACG CCCACGCCTG TCACGGCCAT TGGGCCCCCC 
ACCACGGCCA TCCAGGAGCC ACCGTCCAGA ATTGTGCCCA CCCCCACGTC TCCAGCCATT 
GCTCCTCCAA CCGAGACCAT GGCTCCTCCG GTCAGGGACC CTGTTCCCGG GAAGCCCACA 
GTCACCATTC GGACTCGAGG TGCCATTATT CAGACCCCAA CCCTTGGCCC CATCCAGCCC 
ACTCGGGTGT CAGAAGCTGG CACCACAGTT CCCAGCCAGA TCCGCCCAAC AATGACCATT 
CCTGGTTACA TGGAGCCCAC AGCAGTCATC ACCCCTCCTA CGACGACCAC CAAGAAGCCA 
CGAGTGTCCA CGCCCAGACC AGCCACACCT TCTACTGACT CCTCTACCAC CACTACTCGA 
AGGCCAACCA AGAAGCCGCG GACATCCCGA CCGGTGCCCC GGGTCACCAC CAAGGCACCC 
ATCACCAGAC TGGAAACCGC CTCCCCGCCG ACACGCATGC GCACCACCAC CAGCGGGGTA 
CCCCATGGAG GGGAACCCAA CCAGCGTCCA GAGCTCAAGA ACCACATCGA CAGGGTGGAC 
GCCTGGGTCG GCACCTACTT TGAGGTGAAG ATCCCATCGG ATACCTTCTA CGACAATGAG 
GACACCACCA CTGATAAGCT GAAGTTGACC CTGAAGCTTC GGGAGCAGCA GCTGGTAGGC 
GAGAAGTCGT GGGTGCAGTT CAACAGCAAC AGCCAGCTCA TGTATGGCCT GCCAGACAGC 
AGCCACGTGG GCAAGCACGA GTACTTCATG CACGCCACGG ACAAGGGGGG CCTGTCGGCG 
GTGGACGCCT TTGAGATTCA TGTCCACAGG CGCCCGCAAG GGGACAAGGC TCCTGCACGC 
TTCAAGGCTA AGTTGACAGG TGACCCTGCA GCCGTGACGA ACGACATTCA CAAGAAGATT 
GCCCTGGTGA AGAAGCTGGC CTTTGCCTTC GGGGACCGCA ACTGCAGTAC CATCACCCTG 
CAGAACATCA CCCGGGGCTC CATTGTGGTG GAGTGGACCA ACAATACCCT GCCCCTGGAG 
CCCTGCCCCA AGGAGCAGAT CACGGCGCTG AGCCGGAGGA TCGCTGAGGA TGACGGGAAG 
CCTCGAGGCG CCTTCGTCAA CGCACTGGAG CCTGACTTCC AGGCCATGAG CATCACAGTG 
ACGGGCTCTG GCAGCTGCCG GCATCTCCAG TTTGTCCCAG TGGCACCGCC CATGAGGGTG 
CCCTCGGAGG CACCAGCCAC GGAGGTGCCA GACCGGGACC CCGAGAAGAG CAGCGAGGAC 
GATGTGTACT TGCACACGGT CATCCCGGCC GTGGTGGTGG CGGCCATCCT GCTCATCGCG 
GGCATCATTG CCATGATCTG CTATCGCAAG AAGCGGAAGG GCAAGCTCAC CCTGGAGGAC 
CAAGCCACCT TCATCAAGAA GGGGGTGCCC ATTATCTTTG CGGACGAGCT GGACGACTCC 
AAGCCCCCGC CCTCCTCCAG CATGCCACTG ATCCTGCAGG AGGAGAAAGC CCCCCTCCCC 
CCTCCCGAGT ACCCCAACCA GAGCGTGCCC GAGACCACTC CCCTGAACCA GGACACCGTG 
GGAGAGTATG CGCCCCTGCG GGATGAGGAT CCCAGCGCGC CGCCCTACCA GCCCCCGCCG 
CCCTTCACAG CCCCCATGGA GGGCAAGGGC TCCCGGCCCA AGAACATGAC CCCCTACCGG 
TCGCCCCCTC CCTACGTGCC CCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DAG1
Entrez Gene ID	281439
Full Name	dystroglycan 1
Synonyms	RAB7
General protein information	Preferred Names dystroglycan 1 Names dystroglycan RAB7, member RAS oncogene family dystrophin-associated glycoprotein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	DAG1	NP_776587.1
Danio rerio (zebrafish)	dag1	NP_775381.1
Canis lupus familiaris (dog)	DAG1	NP_001029164.1
Homo sapiens (human)	DAG1	NP_001171105.1
Mus musculus (house mouse)	Dag1	NP_001263421.1
Rattus norvegicus (Norway rat)	Dag1	NP_446149.1
Pan troglodytes (chimpanzee)	DAG1	XP_003339091.1
	DAG1	XP_001108436.1
Gallus gallus (chicken)	DAG1	NP_001091009.1
Xenopus tropicalis (tropical clawed frog)	dag1	NP_001016518.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DAG1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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