PRM2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRM2
Entrez Gene ID	281424
Full Name	protamine 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984645.1	XP_024840413.1	protamine-2 isoform X1
NM_174157.4	NP_776582.2	protamine-2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Differential expression of protamine 1 and 2 genes in mature spermatozoa of normal and motility impaired semen producing crossbred Frieswal (HF?Sahiwal) bulls.
Ganguly I, Gaur GK, Kumar S, Mandal DK, Kumar M, Singh U, Kumar S, Sharma A
Research in veterinary science94(2)256-62(2013 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The bovine protamine 2 gene: evidence for alternative splicing.
Kremling H, Reinhart N, Schl?sser M, Engel W
Biochimica et biophysica acta1132(2)133-9(1992 Sep)

The lack of protamine 2 (P2) in boar and bull spermatozoa is due to mutations within the P2 gene.
Maier WM, Nussbaum G, Domenjoud L, Klemm U, Engel W
Nucleic acids research18(5)1249-54(1990 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study was conducted to isolate high-quality RNA from mature spermatozoa and to monitor the expression profile of PRM1 and PRM2 gene in ejaculated spermatozoa of normal and motility impaired bulls.
Title: Differential expression of protamine 1 and 2 genes in mature spermatozoa of normal and motility impaired semen producing crossbred Frieswal (HF?Sahiwal) bulls.

The following PRM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa00974	NM_174157.4 Latest version!	Bos taurus protamine 2 (PRM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa230143	XM_024984645.1 Latest version!	Bos taurus protamine 2 (PRM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRM2 ( NM_174157.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776582.2 Bos taurus protamine 2 (PRM2), mRNA.

Price & Availability↑

CloneID	OBa00974
Clone ID Related Accession (Same CDS sequence)	NM_174157.4
Accession Version	NM_174157.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Bos taurus(cattle)
Product	protamine-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC109783.1. On Aug 31, 2012 this sequence version replaced NM_174157.3. ##Evidence-Data-START## Transcript exon combination :: BC109783.1, DT830072.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145420, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCGAT GCCACGTGAA GAGTCCAACT GAAAGTCCAC CCGGGCAGCA GGGCTCCGGG 
CAGCAGGGCG AGACGGAGCA CCCGGATCAG GCACGGGAGC TGAGGCCGGA AGACATCCCG 
GTCTACGGGA GGACTCACAG AGGCCGCTAC CACTACAGAC ACAGGAGCCA CACGCGGCGG 
CGCCCCTACC GGAGGCGCCG GAGAAGGGCC TGCAGGCACA GGAGGCGCCG CAGAGGCTGC 
CGAAGGATGA GGAGGAGGAG GAGGAGATGC GGAAGGCAGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776582.2
CDS	123..407
Translation	MVRCHVKSPTESPPGQQGSGQQGETEHPDQARELRPEDIPVYGRTHRGRYHYRHRSHTRRRPYRRRRRRACRHRRRRRGCRRMRRRRRRCGRQL

Target ORF information:

RefSeq Version	NM_174157.4
Organism	Bos taurus(cattle)
Definition	Bos taurus protamine 2 (PRM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174157.4

ORF Insert Sequence:

ATGGTCCGAT GCCACGTGAA GAGTCCAACT GAAAGTCCAC CCGGGCAGCA GGGCTCCGGG 
CAGCAGGGCG AGACGGAGCA CCCGGATCAG GCACGGGAGC TGAGGCCGGA AGACATCCCG 
GTCTACGGGA GGACTCACAG AGGCCGCTAC CACTACAGAC ACAGGAGCCA CACGCGGCGG 
CGCCCCTACC GGAGGCGCCG GAGAAGGGCC TGCAGGCACA GGAGGCGCCG CAGAGGCTGC 
CGAAGGATGA GGAGGAGGAG GAGGAGATGC GGAAGGCAGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRM2 ( XM_024984645.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840413.1 Bos taurus protamine 2 (PRM2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230143
Clone ID Related Accession (Same CDS sequence)	XM_024984645.1
Accession Version	XM_024984645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protamine-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGGAA TTTGTGGAAT TCCTGGCACA GAGCTGCGGA CAGACCCGAC CAACACTGTT 
AACAGCAAGA GCAGGGGGCA GGCCTCAGCC TCCTCCCCGT CCCCCCAAGG CCAGCTGCCG 
TCTCAGCCCG AGTCGCCCAC CCGTGACCCC TCCATCACGA TGGTCCGATG CCACGTGAAG 
AGTCCAACTG AAAGTCCACC CGGGCAGCAG GGCTCCGGGC AGCAGGGCGA GACGGAGCAC 
CCGGATCAGG CACGGGAGCT GAGGCCGGAA GACATCCCGG TCTACGGGAG GACTCACAGA 
GGCCGCTACC ACTACAGACA CAGGAGCCAC ACGCGGCGGC GCCCCTACCG GAGGCGCCGG 
AGAAGGGCCT GCAGGCACAG GAGGCGCCGC AGAGGCTGCC GAAGGATGAG GAGGAGGAGG 
AGGAGATGCA GAAGGCAGCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840413.1
CDS	49..492
Translation	MYGICGIPGTELRTDPTNTVNSKSRGQASASSPSPQGQLPSQPESPTRDPSITMVRCHVKSPTESPPGQQGSGQQGETEHPDQARELRPEDIPVYGRTHRGRYHYRHRSHTRRRPYRRRRRRACRHRRRRRGCRRMRRRRRRCRRQL

Target ORF information:

RefSeq Version	XM_024984645.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protamine 2 (PRM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984645.1

ORF Insert Sequence:

ATGTATGGAA TTTGTGGAAT TCCTGGCACA GAGCTGCGGA CAGACCCGAC CAACACTGTT 
AACAGCAAGA GCAGGGGGCA GGCCTCAGCC TCCTCCCCGT CCCCCCAAGG CCAGCTGCCG 
TCTCAGCCCG AGTCGCCCAC CCGTGACCCC TCCATCACGA TGGTCCGATG CCACGTGAAG 
AGTCCAACTG AAAGTCCACC CGGGCAGCAG GGCTCCGGGC AGCAGGGCGA GACGGAGCAC 
CCGGATCAGG CACGGGAGCT GAGGCCGGAA GACATCCCGG TCTACGGGAG GACTCACAGA 
GGCCGCTACC ACTACAGACA CAGGAGCCAC ACGCGGCGGC GCCCCTACCG GAGGCGCCGG 
AGAAGGGCCT GCAGGCACAG GAGGCGCCGC AGAGGCTGCC GAAGGATGAG GAGGAGGAGG 
AGGAGATGCA GAAGGCAGCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential expression of protamine 1 and 2 genes in mature spermatozoa of normal and motility impaired semen producing crossbred Frieswal (HF?Sahiwal) bulls.
Research in veterinary science94(2)256-62(2013 Apr)
Ganguly I,Gaur GK,Kumar S,Mandal DK,Kumar M,Singh U,Kumar S,Sharma A

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The bovine protamine 2 gene: evidence for alternative splicing.
Biochimica et biophysica acta1132(2)133-9(1992 Sep)
Kremling H,Reinhart N,Schl?sser M,Engel W

The lack of protamine 2 (P2) in boar and bull spermatozoa is due to mutations within the P2 gene.
Nucleic acids research18(5)1249-54(1990 Mar)
Maier WM,Nussbaum G,Domenjoud L,Klemm U,Engel W

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PRM2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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