NCF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCF1
Entrez Gene ID	281345
Full Name	neutrophil cytosolic factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174119.4	NP_776544.1	neutrophil cytosol factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCF1	NP_000256.4
Canis lupus familiaris (dog)	NCF1	XP_849574.2
Mus musculus (house mouse)	Ncf1	NP_001272966.1
Rattus norvegicus (Norway rat)	Ncf1	NP_446186.2
Xenopus tropicalis (tropical clawed frog)	ncf1	NP_001106375.1
	NCF1	XP_001084055.1
Gallus gallus (chicken)	NCF1	NP_001025880.1
Pan troglodytes (chimpanzee)	NCF1	XP_001152639.1
Bos taurus (cattle)	NCF1	NP_776544.1
Danio rerio (zebrafish)	ncf1	NP_001025242.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress.
Siu KL, Gao L, Cai H
The Journal of biological chemistry291(16)8653-62(2016 Apr)

Genetic mutations potentially cause two novel NCF1 splice variants up-regulated in the mammary gland, blood and neutrophil of cows infected by Escherichia coli.
Zhang Z, Wang X, Li R, Ju Z, Qi C, Zhang Y, Guo F, Luo G, Li Q, Wang C, Zhong J, Xu Y, Huang J, Xu Y
Microbiological research17424-32(2015 May)

Peroxide generation by p47phox-Src activation of Nox2 has a key role in protein kinase C-induced arterial smooth muscle contraction.
Gupte SA, Kaminski PM, George S, Kouznestova L, Olson SC, Mathew R, Hintze TH, Wolin MS
American journal of physiology. Heart and circulatory physiology296(4)H1048-57(2009 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress.
Title: Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress.

two novel splice variants designed as NCF1-TV1 (retained intron 6) and NCF1-TV2 (retained part of intron 8)encoding two putative truncated proteins (239AA and 283AA). Two splice variants were up-regulated in the mastitis-infected cows' mammary tissues, blood and neutrophils compared with these of healthy cows.
Title: Genetic mutations potentially cause two novel NCF1 splice variants up-regulated in the mammary gland, blood and neutrophil of cows infected by Escherichia coli.

A p47(phox) and Src kinase activation of peroxide production by Nox2 appears to be an important contributor to vascular contractile mechanisms mediated through activation of protein kinase C.
Title: Peroxide generation by p47phox-Src activation of Nox2 has a key role in protein kinase C-induced arterial smooth muscle contraction.

The following NCF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44774	NM_174119.4 Latest version!	Bos taurus neutrophil cytosolic factor 1 (NCF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCF1 ( NM_174119.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776544.1 Bos taurus neutrophil cytosolic factor 1 (NCF1), mRNA.

Price & Availability↑

CloneID	OBa44774
Clone ID Related Accession (Same CDS sequence)	NM_174119.4
Accession Version	NM_174119.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	neutrophil cytosol factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103145.1. On Aug 30, 2012 this sequence version replaced NM_174119.3. ##Evidence-Data-START## Transcript exon combination :: BC103145.1, BT020852.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGACC ACTTCATCCG CCACATTGCC CTCCTGGGCT TCGAGAAGCG CTTCGTCCCC 
AGCCAGCACT ACGTCTACAT GTTCCTGGTG AAATGGCAGG ACCTGTCTGA GAAGGTGGTC 
TATCGGCGCT TCACCGAGAT CTACGAATTC CACAAAATCT TAAAGGAGAT GTTTCCTATT 
GAGGCGGGGG ACATCAACCC AGAGAATAGG ATCATCCCGC ACCTGCCAGC CCCGCGGTGG 
TATGATGGGC AGCGGGTGGC CGAGAGCCGC CAGGGCACTC TTACCGAGTA CTGCAGCACG 
CTCATGAGCC TGCCTGTCAA GATCTCCCGC TGCCCGCACC TCCTCAACTT CTTCAAGGTG 
CGCCCCGACG ACCTCAAGCT CCCCACAGAC AGCCAGGTGA AAAAGCCAGA GACATACCTG 
ATGCCCAAAG ATGGCAAGAA CAACGCTGCG GACATCACGG GCCCCATCAT CCTGCAGACG 
TACCGCGCCA TCGCTGACTA CGAGAAGGGC TCGAGTTCCC AAATGGCTCT GGCCACGGGC 
GACGTGGTGG ACGTCGTGGA GAAGAACGAG AGCGGCTGGT GGTTCTGCCA AATGAAGACA 
AAGCGTGGCT GGGTCCCAGC GTCCTACTTG GAGCCTCTGG ACAGTCCTGA TGAAGCCGAG 
GACCCAGAAC CCAACTATGC AGGTGAGCCC TACATCACCA TCAAAGCCTA CACTGCTGTA 
CTGGAGGATG AGATATCCTT GGAGGAGGGT GAAGCCATTG AGGTCATTCA TAAGCTCCTG 
GATGGCTGGT GGGTCATCAG GAAAGAAGAC GTCACAGGTT ACTTTCCATC CATGTACCTG 
CAGAAGGCAG GGCAGGACGT AGCCCAGGCC AAAAGCCAGA TCAAGAGCCG GGGGGCGCCA 
CCCCGCAGGT CGTCCATCCG CAATGCTCAC AGCATCCACC AGCGGTCACG GAAGCGCCTC 
AGCCAGGACA CCTATCGGCG CAACAGCGTC CGTTTTATGC AGCAGCGCCG CCACCAGCGG 
CCGGAGCCAC AGCGCTCCAG GAGCGCCCTG AGGGAGCAGC AGCAACCCAA GACCGAGCGC 
CCCAAGCCGC AGCCGGCCGT GCCCCCCAGG CCCAGCGCAG ACCTCATCCT GCACCGCTGC 
AGCGAGAGCA CCAAGCGGAA GCTGGCCTCT GCCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776544.1
CDS	30..1208
Translation	MGDHFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKILKEMFPIEAGDINPENRIIPHLPAPRWYDGQRVAESRQGTLTEYCSTLMSLPVKISRCPHLLNFFKVRPDDLKLPTDSQVKKPETYLMPKDGKNNAADITGPIILQTYRAIADYEKGSSSQMALATGDVVDVVEKNESGWWFCQMKTKRGWVPASYLEPLDSPDEAEDPEPNYAGEPYITIKAYTAVLEDEISLEEGEAIEVIHKLLDGWWVIRKEDVTGYFPSMYLQKAGQDVAQAKSQIKSRGAPPRRSSIRNAHSIHQRSRKRLSQDTYRRNSVRFMQQRRHQRPEPQRSRSALREQQQPKTERPKPQPAVPPRPSADLILHRCSESTKRKLASAV

Target ORF information:

RefSeq Version	NM_174119.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neutrophil cytosolic factor 1 (NCF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174119.4

ORF Insert Sequence:

ATGGGGGACC ACTTCATCCG CCACATTGCC CTCCTGGGCT TCGAGAAGCG CTTCGTCCCC 
AGCCAGCACT ACGTCTACAT GTTCCTGGTG AAATGGCAGG ACCTGTCTGA GAAGGTGGTC 
TATCGGCGCT TCACCGAGAT CTACGAATTC CACAAAATCT TAAAGGAGAT GTTTCCTATT 
GAGGCGGGGG ACATCAACCC AGAGAATAGG ATCATCCCGC ACCTGCCAGC CCCGCGGTGG 
TATGATGGGC AGCGGGTGGC CGAGAGCCGC CAGGGCACTC TTACCGAGTA CTGCAGCACG 
CTCATGAGCC TGCCTGTCAA GATCTCCCGC TGCCCGCACC TCCTCAACTT CTTCAAGGTG 
CGCCCCGACG ACCTCAAGCT CCCCACAGAC AGCCAGGTGA AAAAGCCAGA GACATACCTG 
ATGCCCAAAG ATGGCAAGAA CAACGCTGCG GACATCACGG GCCCCATCAT CCTGCAGACG 
TACCGCGCCA TCGCTGACTA CGAGAAGGGC TCGAGTTCCC AAATGGCTCT GGCCACGGGC 
GACGTGGTGG ACGTCGTGGA GAAGAACGAG AGCGGCTGGT GGTTCTGCCA AATGAAGACA 
AAGCGTGGCT GGGTCCCAGC GTCCTACTTG GAGCCTCTGG ACAGTCCTGA TGAAGCCGAG 
GACCCAGAAC CCAACTATGC AGGTGAGCCC TACATCACCA TCAAAGCCTA CACTGCTGTA 
CTGGAGGATG AGATATCCTT GGAGGAGGGT GAAGCCATTG AGGTCATTCA TAAGCTCCTG 
GATGGCTGGT GGGTCATCAG GAAAGAAGAC GTCACAGGTT ACTTTCCATC CATGTACCTG 
CAGAAGGCAG GGCAGGACGT AGCCCAGGCC AAAAGCCAGA TCAAGAGCCG GGGGGCGCCA 
CCCCGCAGGT CGTCCATCCG CAATGCTCAC AGCATCCACC AGCGGTCACG GAAGCGCCTC 
AGCCAGGACA CCTATCGGCG CAACAGCGTC CGTTTTATGC AGCAGCGCCG CCACCAGCGG 
CCGGAGCCAC AGCGCTCCAG GAGCGCCCTG AGGGAGCAGC AGCAACCCAA GACCGAGCGC 
CCCAAGCCGC AGCCGGCCGT GCCCCCCAGG CCCAGCGCAG ACCTCATCCT GCACCGCTGC 
AGCGAGAGCA CCAAGCGGAA GCTGGCCTCT GCCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress.
The Journal of biological chemistry291(16)8653-62(2016 Apr)
Siu KL,Gao L,Cai H

Genetic mutations potentially cause two novel NCF1 splice variants up-regulated in the mammary gland, blood and neutrophil of cows infected by Escherichia coli.
Microbiological research17424-32(2015 May)
Zhang Z,Wang X,Li R,Ju Z,Qi C,Zhang Y,Guo F,Luo G,Li Q,Wang C,Zhong J,Xu Y,Huang J,Xu Y

Peroxide generation by p47phox-Src activation of Nox2 has a key role in protein kinase C-induced arterial smooth muscle contraction.
American journal of physiology. Heart and circulatory physiology296(4)H1048-57(2009 Apr)
Gupte SA,Kaminski PM,George S,Kouznestova L,Olson SC,Mathew R,Hintze TH,Wolin MS

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

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NCF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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