GPX3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPX3
Entrez Gene ID	281210
Full Name	glutathione peroxidase 3
General protein information	Preferred Names glutathione peroxidase 3 Names glutathione peroxidase 3 GPx-3 GPx-P GSHPx-3 GSHPx-P glutathione peroxidase 3 (plasma) plasma glutathione peroxidase
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_174077.4	NP_776502.1	glutathione peroxidase 3 precursor
NM_174077.5	NP_776502.1	glutathione peroxidase 3 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GPX3	NP_001157926.1
Gallus gallus (chicken)	GPX3	NP_001156704.1
	GPX3	NP_001152772.1
Mus musculus (house mouse)	Gpx3	NP_032187.2
Homo sapiens (human)	GPX3	NP_002075.2
Rattus norvegicus (Norway rat)	Gpx3	NP_071970.2
Danio rerio (zebrafish)	gpx3	NP_001131027.1
Xenopus tropicalis (tropical clawed frog)	gpx3	NP_988961.2
Pan troglodytes (chimpanzee)	GPX3	NP_001108629.1
Bos taurus (cattle)	GPX3	NP_776502.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered expression of inflammation-associated genes in oviductal cells following follicular fluid exposure: implications for ovarian carcinogenesis.
Lau A, Kollara A, St John E, Tone AA, Virtanen C, Greenblatt EM, King WA, Brown TJ
Experimental biology and medicine (Maywood, N.J.)239(1)24-32(2014 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Cloning of the bovine plasma selenium-dependent glutathione peroxidase (GP) cDNA from the ocular ciliary epithelium: expression of the plasma and cellular forms within the mammalian eye.
Mart?n-Alonso JM, Ghosh S, Coca-Prados M
Journal of biochemistry114(2)284-91(1993 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected.
Title: Altered expression of inflammation-associated genes in oviductal cells following follicular fluid exposure: implications for ovarian carcinogenesis.

The following GPX3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPX3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa134038	NM_174077.4 Latest version!	Bos taurus glutathione peroxidase 3 (GPX3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OBa239254	NM_174077.5 Latest version!	Bos taurus glutathione peroxidase 3 (GPX3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPX3 ( NM_174077.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776502.1 Bos taurus glutathione peroxidase 3 (GPX3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa134038
Clone ID Related Accession (Same CDS sequence)	NM_174077.4
Accession Version	NM_174077.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	glutathione peroxidase 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DV838871.1, EV679018.1, EV688099.1 and CO879048.1. On Aug 1, 2016 this sequence version replaced NM_174077.3. Transcript Variant: This variant (1) represents the protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: BC149266.1, L10325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02403641, SAMN02822088 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal protein contains selenocysteine :: PMID: 8262911 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGGC TCTTCCGGGC ATCCTGCCTT CTCTCCCTGC TCCTGGCCGG CTTCATTCCC 
CCGAGCCAGG GACAGGAGAA GTCGAAGACG GACTGCCACG CTGGTGTGGG TGGCACCATC 
TATGAGTACG GGGCCCTCAC CATCGATGGG GAGGAGTACA TCCCCTTTAA GCAGTACGCT 
GGCAAATACA TCCTCTTCGT CAACGTGGCC AGCTACTGAG GCCTGACGGG CCAGTACGTT 
GAACTGAATG CACTGCAGGA AGAGCTTGAA CCATTTGGTC TGGTCATTCT GGGCTTCCCC 
TGCAACCAAT TTGGAAAACA AGAACCAGGA GAGAACTCGG AGATCCTAGC CACCCTCAAG 
TATGTTCGAC CAGGTGGGGG CTTCACCCCC AATTTCCAAC TGTTTGAGAA AGGCGATGTG 
AATGGGGAGA AAGAGCAGAA GTTCTACACG TTCCTGAAGA ACTCCTGTCC TCCTACCTCG 
GAGCTCCTAG GCTCACCTGA CCGCCTTTTC TGGGAACCCA TGAAGGTCCA TGACATCCGG 
TGGAACTTTG AGAAGTTCCT GGTGGGGCCA GACGGCATCC CCATCATGCG CTGGTACCAC 
CGCACCACGG TCAACTCTGT CAAGATGGAC ATCCTGACCT ACATGCGGCG GCGGGCGGTC 
TGGGAGGCCA AGGGGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776502.1
CDS	87..767
Translation	MARLFRASCLLSLLLAGFIPPSQGQEKSKTDCHAGVGGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASY*GLTGQYVELNALQEELEPFGLVILGFPCNQFGKQEPGENSEILATLKYVRPGGGFTPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGSPDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWYHRTTVNSVKMDILTYMRRRAVWEAKGK

Target ORF information:

RefSeq Version	NM_174077.4
Organism	Bos taurus(cattle)
Definition	Bos taurus glutathione peroxidase 3 (GPX3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174077.4

ORF Insert Sequence:

ATGGCCCGGC TCTTCCGGGC ATCCTGCCTT CTCTCCCTGC TCCTGGCCGG CTTCATTCCC 
CCGAGCCAGG GACAGGAGAA GTCGAAGACG GACTGCCACG CTGGTGTGGG TGGCACCATC 
TATGAGTACG GGGCCCTCAC CATCGATGGG GAGGAGTACA TCCCCTTTAA GCAGTACGCT 
GGCAAATACA TCCTCTTCGT CAACGTGGCC AGCTACTGAG GCCTGACGGG CCAGTACGTT 
GAACTGAATG CACTGCAGGA AGAGCTTGAA CCATTTGGTC TGGTCATTCT GGGCTTCCCC 
TGCAACCAAT TTGGAAAACA AGAACCAGGA GAGAACTCGG AGATCCTAGC CACCCTCAAG 
TATGTTCGAC CAGGTGGGGG CTTCACCCCC AATTTCCAAC TGTTTGAGAA AGGCGATGTG 
AATGGGGAGA AAGAGCAGAA GTTCTACACG TTCCTGAAGA ACTCCTGTCC TCCTACCTCG 
GAGCTCCTAG GCTCACCTGA CCGCCTTTTC TGGGAACCCA TGAAGGTCCA TGACATCCGG 
TGGAACTTTG AGAAGTTCCT GGTGGGGCCA GACGGCATCC CCATCATGCG CTGGTACCAC 
CGCACCACGG TCAACTCTGT CAAGATGGAC ATCCTGACCT ACATGCGGCG GCGGGCGGTC 
TGGGAGGCCA AGGGGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPX3 ( NM_174077.5 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776502.1 Bos taurus glutathione peroxidase 3 (GPX3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa239254
Clone ID Related Accession (Same CDS sequence)	NM_174077.5
Accession Version	NM_174077.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Bos taurus(cattle)
Product	glutathione peroxidase 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DV838871.1, BC149266.1, DT861549.1, DV850873.1 and EV688099.1. On Jun 19, 2018 this sequence version replaced NM_174077.4. Transcript Variant: This variant (1) represents the selenoprotein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: BC149266.1, SRR5571254.533.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal protein contains selenocysteine :: PMID: 8262911 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGGC TCTTCCGGGC ATCCTGCCTT CTGTCCCTGC TCCTGGCCGG CTTCATTCCC 
CCGAGCCAGG GACAGGAGAA GTCGAAGACG GACTGCCACG CTGGTGTGGG TGGCACCATC 
TATGAGTACG GGGCCCTCAC CATCGATGGG GAGGAGTACA TCCCCTTTAA GCAGTACGCT 
GGCAAATACA TCCTCTTCGT CAACGTGGCC AGCTACTGAG GCCTGACGGG CCAGTACGTT 
GAACTGAATG CACTGCAGGA AGAGCTTGAA CCATTTGGTC TGGTCATTCT GGGCTTCCCC 
TGCAACCAAT TTGGAAAACA AGAACCAGGA GAGAACTCGG AGATCCTAGC CACCCTCAAG 
TATGTTCGAC CAGGTGGGGG CTTCACCCCC AATTTCCAAC TGTTTGAGAA AGGCGATGTG 
AATGGGGAGA AAGAGCAGAA GTTCTACACG TTCCTGAAGA ACTCCTGTCC TCCTACCTCG 
GAGCTCCTAG GCTCACCTGA CCGCCTTTTC TGGGAACCCA TGAAGGTCCA TGACATCCGG 
TGGAACTTTG AGAAGTTCCT GGTGGGGCCA GACGGCATCC CCATCATGCG CTGGTACCAC 
CGCACCACGG TCAACTCTGT CAAGATGGAC ATCCTGACCT ACATGCGGCG GCGGGCGGTC 
TGGGAGGCCA AGGGGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776502.1
CDS	87..767
Translation	MARLFRASCLLSLLLAGFIPPSQGQEKSKTDCHAGVGGTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASY*GLTGQYVELNALQEELEPFGLVILGFPCNQFGKQEPGENSEILATLKYVRPGGGFTPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGSPDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWYHRTTVNSVKMDILTYMRRRAVWEAKGK

Target ORF information:

RefSeq Version	NM_174077.5
Organism	Bos taurus(cattle)
Definition	Bos taurus glutathione peroxidase 3 (GPX3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174077.5

ORF Insert Sequence:

ATGGCCCGGC TCTTCCGGGC ATCCTGCCTT CTGTCCCTGC TCCTGGCCGG CTTCATTCCC 
CCGAGCCAGG GACAGGAGAA GTCGAAGACG GACTGCCACG CTGGTGTGGG TGGCACCATC 
TATGAGTACG GGGCCCTCAC CATCGATGGG GAGGAGTACA TCCCCTTTAA GCAGTACGCT 
GGCAAATACA TCCTCTTCGT CAACGTGGCC AGCTACTGAG GCCTGACGGG CCAGTACGTT 
GAACTGAATG CACTGCAGGA AGAGCTTGAA CCATTTGGTC TGGTCATTCT GGGCTTCCCC 
TGCAACCAAT TTGGAAAACA AGAACCAGGA GAGAACTCGG AGATCCTAGC CACCCTCAAG 
TATGTTCGAC CAGGTGGGGG CTTCACCCCC AATTTCCAAC TGTTTGAGAA AGGCGATGTG 
AATGGGGAGA AAGAGCAGAA GTTCTACACG TTCCTGAAGA ACTCCTGTCC TCCTACCTCG 
GAGCTCCTAG GCTCACCTGA CCGCCTTTTC TGGGAACCCA TGAAGGTCCA TGACATCCGG 
TGGAACTTTG AGAAGTTCCT GGTGGGGCCA GACGGCATCC CCATCATGCG CTGGTACCAC 
CGCACCACGG TCAACTCTGT CAAGATGGAC ATCCTGACCT ACATGCGGCG GCGGGCGGTC 
TGGGAGGCCA AGGGGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered expression of inflammation-associated genes in oviductal cells following follicular fluid exposure: implications for ovarian carcinogenesis.
Experimental biology and medicine (Maywood, N.J.)239(1)24-32(2014 Jan)
Lau A,Kollara A,St John E,Tone AA,Virtanen C,Greenblatt EM,King WA,Brown TJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Cloning of the bovine plasma selenium-dependent glutathione peroxidase (GP) cDNA from the ocular ciliary epithelium: expression of the plasma and cellular forms within the mammalian eye.
Journal of biochemistry114(2)284-91(1993 Aug)
Mart?n-Alonso JM,Ghosh S,Coca-Prados M

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GPX3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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