GJA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJA1
Entrez Gene ID	281193
Full Name	gap junction protein alpha 1
General protein information	Preferred Names gap junction protein alpha 1 Names gap junction alpha-1 protein connexin-43 cx43 gap junction protein, alpha 1, 43 kD (connexin 43) gap junction protein, alpha 1, 43kDa vascular smooth muscle connexin-43
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 9 Map Location: 9q15-q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_174068.2	NP_776493.1	gap junction alpha-1 protein

Pathways from BioSystems

Homologies

	GJA1	XP_001109617.1
Mus musculus (house mouse)	Gja1	NP_034418.1
Xenopus tropicalis (tropical clawed frog)	gja1	NP_988856.1
Pan troglodytes (chimpanzee)	GJA1	XP_001164338.1
Canis lupus familiaris (dog)	GJA1	NP_001002951.2
Bos taurus (cattle)	GJA1	NP_776493.1
Rattus norvegicus (Norway rat)	Gja1	NP_036699.1
Homo sapiens (human)	GJA1	NP_000156.1
Gallus gallus (chicken)	GJA1	NP_989917.1
Danio rerio (zebrafish)	cx43	NP_571113.1

Gene Ontology
Bibliography

Related articles in PubMed

Estradiol Reduces Connexin43 Gap Junctions in the Uterus during Adenomyosis in Cows.
Korzekwa AJ, ?upicka M, Socha BM, Szczepa?ska AA
Polish journal of veterinary sciences19(3)609-617(2016 Sep)

Ketone bodies upregulate endothelial connexin 43 (Cx43) gap junctions.
Ho CF, Chan KW, Yeh HI, Kuo J, Liu HJ, Wang CY
Veterinary journal (London, England : 1997)198(3)696-701(2013 Dec)

RhoA GTPase switch controls Cx43-hemichannel activity through the contractile system.
Ponsaerts R, D'hondt C, Hertens F, Parys JB, Leybaert L, Vereecke J, Himpens B, Bultynck G
PloS one7(7)e42074(2012)

Expression of gap junction protein connexin 43 in bovine urinary bladder tumours.
Corteggio A, Florio J, Roperto F, Borzacchiello G
Journal of comparative pathology144(1)86-90(2011 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study found that down-regulation of Cx43 expression in the junction zone might play an important role in pathogenesis of adenomyosis, and that estradiol modulates gap junctions during adenomyosis.
Title: Estradiol Reduces Connexin43 Gap Junctions in the Uterus during Adenomyosis in Cows.

Cx43 mRNA and protein expression increased after endothelial cell exposure to ketone bodies; this was accompanied by upregulation of gap junctional intercellular coupling and cell migration.
Title: Ketone bodies upregulate endothelial connexin 43 (Cx43) gap junctions.

RhoA appears to be an important molecular switch that controls Cx43 hemichannel openings and hemichannel-mediated ATP-dependent paracrine intercellular communication under (patho)physiological conditions of stress
Title: RhoA GTPase switch controls Cx43-hemichannel activity through the contractile system.

Papillary urothelial carcinomas showed moderate cytoplasmic and membrane labelling, while invasive carcinoma showed loss of connexin 43 expression.
Title: Expression of gap junction protein connexin 43 in bovine urinary bladder tumours.

Results describe the effect of suppression of connexin 43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo.
Title: Suppression of connexin 43 and E-cadherin transcripts in in vitro derived bovine embryos following culture in vitro or in vivo in the homologous bovine oviduct.

The following GJA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43196	NM_174068.2 Latest version!	Bos taurus gap junction protein alpha 1 (GJA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJA1 ( NM_174068.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776493.1 Bos taurus gap junction protein alpha 1 (GJA1), mRNA.

Price & Availability↑

CloneID	OBa43196
Clone ID Related Accession (Same CDS sequence)	NM_174068.2
Accession Version	NM_174068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	gap junction alpha-1 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105464.1. On Feb 19, 2006 this sequence version replaced NM_174068.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105464.1, EE335325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGACT GGAGTGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA CTCCACGGCT 
GGAGGGAAGG TGTGGCTGTC AGTCCTTTTC ATTTTCCGAA TCCTGCTACT GGGGACAGCG 
GTTGAGTCAG CCTGGGGTGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA ACAACCTGGT 
TGTGAAAATG TCTGCTATGA CAAATCCTTC CCAATCTCTC ATGTGCGCTT CTGGGTCCTG 
CAGATCATAT TTGTGTCTGT TCCCACACTC CTGTACCTGG CTCATGTGTT CTACGTGATG 
CGAAAGGAAG AGAAGCTGAA CAAGAAGGAG GAGGAACTCA AAGTTGTTGC CCAAACTGAT 
GGTGCCAACG TGGACATGCA CTTGAAGCAG ATTGAAATTA AGAAGTTCAA GTATGGCATT 
GAAGAGCACG GCAAGGTGAA GATGCGAGGG GGCTTGCTGA GAACCTACAT CATCAGTATC 
CTCTTCAAGT CTGTCTTCGA GGTGGCCTTC TTGCTGATCC AGTGGTACAT CTATGGGTTC 
AGCTTGAGTG CCGTTTACAC TTGCAAAAGA GATCCCTGCC CACATCAGGT GGACTGTTTC 
CTTTCTCGGC CCACGGAGAA AACCATCTTC ATCATCTTCA TGCTTGTCGT GTCATTGGTG 
TCTCTTGCCT TGAACATCAT CGAACTCTTC TATGTCTTCT TCAAGGGTGT TAAGGATCGT 
GTGAAGGGAA AGAGCGATCC TTACCACACT ACCACTGGCC CACTGAGCCC CTCCAAAGAC 
TGTGGATCTC CAAAATATGC TTATTTCAAT GGCTGCTCCT CCCCAACCGC TCCTCTCTCG 
CCCATGTCTC CTCCCGGGTA CAAGCTGGTC ACCGGAGACA GAAACAATTC TTCCTGCCGC 
AATTACAACA AACAAGCAAG TGAGCAAAAC TGGGCCAATT ACAGCGCAGA ACAAAATCGA 
ATGGGGCAGG CAGGCAGCAC CATCTCTAAC TCCCACGCAC AGCCTTTTGA TTTCCCAGAC 
GACCACCAGA ATTCTAAAAA GCTTGATGCT GGCCACGAAC TACAGCCTCT CGCCATTGTG 
GACCAGCGGC CTTCCAGCAG AGCCAGCAGT CGCGCCAGCA GCCGACCCCG GCCTGATGAC 
CTAGAGATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776493.1
CDS	206..1357
Translation	MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVVAQTDGANVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHTTTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDHQNSKKLDAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Target ORF information:

RefSeq Version	NM_174068.2
Organism	Bos taurus(cattle)
Definition	Bos taurus gap junction protein alpha 1 (GJA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174068.2

ORF Insert Sequence:

ATGGGTGACT GGAGTGCCTT AGGCAAACTC CTTGACAAGG TTCAAGCCTA CTCCACGGCT 
GGAGGGAAGG TGTGGCTGTC AGTCCTTTTC ATTTTCCGAA TCCTGCTACT GGGGACAGCG 
GTTGAGTCAG CCTGGGGTGA TGAGCAGTCT GCCTTTCGTT GTAACACTCA ACAACCTGGT 
TGTGAAAATG TCTGCTATGA CAAATCCTTC CCAATCTCTC ATGTGCGCTT CTGGGTCCTG 
CAGATCATAT TTGTGTCTGT TCCCACACTC CTGTACCTGG CTCATGTGTT CTACGTGATG 
CGAAAGGAAG AGAAGCTGAA CAAGAAGGAG GAGGAACTCA AAGTTGTTGC CCAAACTGAT 
GGTGCCAACG TGGACATGCA CTTGAAGCAG ATTGAAATTA AGAAGTTCAA GTATGGCATT 
GAAGAGCACG GCAAGGTGAA GATGCGAGGG GGCTTGCTGA GAACCTACAT CATCAGTATC 
CTCTTCAAGT CTGTCTTCGA GGTGGCCTTC TTGCTGATCC AGTGGTACAT CTATGGGTTC 
AGCTTGAGTG CCGTTTACAC TTGCAAAAGA GATCCCTGCC CACATCAGGT GGACTGTTTC 
CTTTCTCGGC CCACGGAGAA AACCATCTTC ATCATCTTCA TGCTTGTCGT GTCATTGGTG 
TCTCTTGCCT TGAACATCAT CGAACTCTTC TATGTCTTCT TCAAGGGTGT TAAGGATCGT 
GTGAAGGGAA AGAGCGATCC TTACCACACT ACCACTGGCC CACTGAGCCC CTCCAAAGAC 
TGTGGATCTC CAAAATATGC TTATTTCAAT GGCTGCTCCT CCCCAACCGC TCCTCTCTCG 
CCCATGTCTC CTCCCGGGTA CAAGCTGGTC ACCGGAGACA GAAACAATTC TTCCTGCCGC 
AATTACAACA AACAAGCAAG TGAGCAAAAC TGGGCCAATT ACAGCGCAGA ACAAAATCGA 
ATGGGGCAGG CAGGCAGCAC CATCTCTAAC TCCCACGCAC AGCCTTTTGA TTTCCCAGAC 
GACCACCAGA ATTCTAAAAA GCTTGATGCT GGCCACGAAC TACAGCCTCT CGCCATTGTG 
GACCAGCGGC CTTCCAGCAG AGCCAGCAGT CGCGCCAGCA GCCGACCCCG GCCTGATGAC 
CTAGAGATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Estradiol Reduces Connexin43 Gap Junctions in the Uterus during Adenomyosis in Cows.
Polish journal of veterinary sciences19(3)609-617(2016 Sep)
Korzekwa AJ,?upicka M,Socha BM,Szczepa?ska AA

Ketone bodies upregulate endothelial connexin 43 (Cx43) gap junctions.
Veterinary journal (London, England : 1997)198(3)696-701(2013 Dec)
Ho CF,Chan KW,Yeh HI,Kuo J,Liu HJ,Wang CY

RhoA GTPase switch controls Cx43-hemichannel activity through the contractile system.
PloS one7(7)e42074(2012)
Ponsaerts R,D'hondt C,Hertens F,Parys JB,Leybaert L,Vereecke J,Himpens B,Bultynck G

Expression of gap junction protein connexin 43 in bovine urinary bladder tumours.
Journal of comparative pathology144(1)86-90(2011 Jan)
Corteggio A,Florio J,Roperto F,Borzacchiello G

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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GJA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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