ECE1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005203070.3	XP_005203127.1	endothelin-converting enzyme 1 isoform X2
XM_015460016.1	XP_015315502.1	endothelin-converting enzyme 1 isoform X1
XM_015460021.2	XP_015315507.1	endothelin-converting enzyme 1 isoform X4
NM_181009.2	NP_851352.1	endothelin-converting enzyme 1
XM_005203070.4	XP_005203127.1	endothelin-converting enzyme 1 isoform X2
XM_010802523.2	XP_010800825.1	endothelin-converting enzyme 1 isoform X3
XM_015460021.1	XP_015315507.1	endothelin-converting enzyme 1 isoform X5
XM_005203072.3	XP_005203129.1	endothelin-converting enzyme 1 isoform X4
XM_015460022.1	XP_015315508.1	endothelin-converting enzyme 1 isoform X6
XM_005203072.4	XP_005203129.1	endothelin-converting enzyme 1 isoform X3
XM_015460022.2	XP_015315508.1	endothelin-converting enzyme 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43600	NM_181009.2 Latest version!	Bos taurus endothelin converting enzyme 1 (ECE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa16887	XM_005203070.4 Latest version!	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa16889	XM_005203072.4 Latest version!	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa45074	XM_010802523.2 Latest version!	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa88045	XM_015460016.1 Latest version!	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa88046	XM_015460021.2 Latest version!	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa88047	XM_015460022.2 Latest version!	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBa16887	XM_005203070.3	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa88046	XM_015460021.1	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa16889	XM_005203072.3	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa88047	XM_015460022.1	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

ECE1 ( NM_181009.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_851352.1 Bos taurus endothelin converting enzyme 1 (ECE1), mRNA.

Price & Availability↑

CloneID	OBa43600
Clone ID Related Accession (Same CDS sequence)	NM_181009.2
Accession Version	NM_181009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U27342.1. On Jun 3, 2003 this sequence version replaced NM_181009.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U27342.1, S73774.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCCCC GGGGGCAGGA TCTGCTGCGG AGCCCCCTCC TCCTGGGCAG CGAGGCCCCT 
GGGCTCACGT CCTCCCCGTT CCGCCTGCCT CCTTCCCTGC AGGTGAACTT CCGAGGCCCC 
CGGAACGGCC AGAGATGCTG GGCCGCCAGG ACCCCGGTGG AGAAGCGGCT GGTGGTGCTG 
GTGGCGCTCC TGGCGGCGGC ATTGGTGGCC TGTTTGGCAG TACTGGGCAT CCAATACCAG 
ACAAGAACGC CCTCGGTGTG CCTAAGTGAG GGCTGCATCT CGGTGACCAG CTCCATCTTG 
AGTTCCATGG ACCCCACGGT GGACCCCTGC CAGGACTTCT TCACCTATGC CTGTGGCGGC 
TGGATCAAAG CCAACCCCGT GCCGGATGGC CACTCGCGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCCAT CATCAAGCAC CTCCTTGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GGAAGGCCCA GGTGTACTAC CGAGCCTGCA TGAACGAAAC CAGGATTGAG 
GAGCTCAAGG CCAAACCCCT GATGGAGCTC ATTGAGAAGC TCGGCGGCTG GAACATCACG 
GGGCCCTGGG ACAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACATC CCACTACCAC 
ACCTCCCCCT TCTTCTCCGT CTACGTCAGT GCCGACTCCA AGAATTCCAA CAGCAACGTG 
ATCCAAGTGG ACCAGTCTGG CCTGGGCTTA CCCTCAAGAG ATTATTACCT GAACAAAACC 
GAGAATGAGA AGGTGCTGAC GGGATACCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGAGGAGGGG CCGAGGACAC CATCCGGCCC CAGATGCAGC AGATCCTGGA CTTTGAGACG 
GCGCTGGCCA ACATCACCAT CCCCCAGGAG AAGCGCCGGG ACGAGGAACT CATCTACCAC 
AAAGTGACGG CGGCTGAGTT GCAGACCTTG GCGCCCGCCA TCAACTGGCT GCCCTTCCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCAGAGC CTATTGTCAT CTACGACAAA 
GAATACCTGA GCAAGGTCTC CACCCTCATC AACAGCACAG ACAAATGCCT GCTGAACAAC 
TACATGATCT GGAACCTGGT ACGGAAGACG AGCTCCTTCC TCGATCAGCG CTTCCAGGAC 
GCCGACGAGA AGTTCATGGA AGTCATGTAT GGGACCAAGA AGACGTGTCT TCCCCGCTGG 
AAGTTTTGTG TGAGTGATAC AGAGAACACC TTGGGCTTCG CCCTGGGCCC CATGTTCGTC 
AAAGCGACCT TCGCTGAGGA CAGCAAGAAC ATAGCCAGCG AGATCATCCT GGAGATCAAG 
AAGGCGTTTG AAGAGAGCCT GAGCACCCTG AAGTGGATGG ATGAAGATAC TCGGAAATCG 
GCCAAGGAAA AGGCGGACGC GATCTACAAC ATGATAGGCT ACCCCAACTT TATCATGGAC 
CCCAAGGAGC TGGACAAAGT GTTCAATGAC TACACCGCTG TGCCAGACCT CTACTTCGAG 
AACGCCATGC GGTTTTTCAA CTTCTCCTGG AGGGTCACTG CCGACCAGCT CCGGAAAGCG 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAACGAGA TCGTGTTTCC GGCCGGAATC CTGCAGGCGC CATTCTACAC CCGCTCTTCA 
CCCAATGCCT TAAACTTCGG CGGCATCGGC GTCGTCGTGG GCCACGAGCT GACTCATGCT 
TTTGATGATC AAGGCCGAGA GTACGACAAG GATGGGAACC TCCGGCCCTG GTGGAAGAAC 
TCGTCCGTGG AGGCGTTCAA GCAGCAGACC GCGTGCATGG TGGAGCAGTA CGGCAACTAT 
AGCGTGAACG GGGAGCCGGT GAACGGCCGG CACACCCTCG GCGAAAACAT CGCCGACAAC 
GGGGGCCTCA AGGCGGCCTA TCGGGCCTAC CAGAACTGGG TCAAGAAGAA TGGGGCTGAG 
CAGACACTGC CCACCCTGGG TCTCACCAAC AACCAGCTCT TCTTCCTGAG TTTTGGACAG 
GTCTGGTGTT CCGTCCGCAC CCCCGAGAGT TCGCACGAAG GTCTCATCAC CGATCCCCAC 
AGCCCCTCCC GCTTCCGGGT CATCGGCTCC ATCTCCAACT CCAAGGAGTT CTCGGAACAC 
TTCCACTGCC CGCCCGGCTC ACCCATGAAC CCGCATCACA AGTGTGAAGT CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851352.1
CDS	118..2394
Translation	MSPRGQDLLRSPLLLGSEAPGLTSSPFRLPPSLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEGCISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFGQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	NM_181009.2
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181009.2

ORF Insert Sequence:

ATGTCTCCCC GGGGGCAGGA TCTGCTGCGG AGCCCCCTCC TCCTGGGCAG CGAGGCCCCT 
GGGCTCACGT CCTCCCCGTT CCGCCTGCCT CCTTCCCTGC AGGTGAACTT CCGAGGCCCC 
CGGAACGGCC AGAGATGCTG GGCCGCCAGG ACCCCGGTGG AGAAGCGGCT GGTGGTGCTG 
GTGGCGCTCC TGGCGGCGGC ATTGGTGGCC TGTTTGGCAG TACTGGGCAT CCAATACCAG 
ACAAGAACGC CCTCGGTGTG CCTAAGTGAG GGCTGCATCT CGGTGACCAG CTCCATCTTG 
AGTTCCATGG ACCCCACGGT GGACCCCTGC CAGGACTTCT TCACCTATGC CTGTGGCGGC 
TGGATCAAAG CCAACCCCGT GCCGGATGGC CACTCGCGCT GGGGGACCTT CAGCAACCTC 
TGGGAACACA ACCAAGCCAT CATCAAGCAC CTCCTTGAAA ACTCCACGGC CAGCGTGAGC 
GAGGCAGAGA GGAAGGCCCA GGTGTACTAC CGAGCCTGCA TGAACGAAAC CAGGATTGAG 
GAGCTCAAGG CCAAACCCCT GATGGAGCTC ATTGAGAAGC TCGGCGGCTG GAACATCACG 
GGGCCCTGGG ACAAGGACAA CTTCCAGGAC ACCCTGCAGG TGGTCACATC CCACTACCAC 
ACCTCCCCCT TCTTCTCCGT CTACGTCAGT GCCGACTCCA AGAATTCCAA CAGCAACGTG 
ATCCAAGTGG ACCAGTCTGG CCTGGGCTTA CCCTCAAGAG ATTATTACCT GAACAAAACC 
GAGAATGAGA AGGTGCTGAC GGGATACCTG AACTACATGG TCCAGCTGGG GAAGCTGCTG 
GGAGGAGGGG CCGAGGACAC CATCCGGCCC CAGATGCAGC AGATCCTGGA CTTTGAGACG 
GCGCTGGCCA ACATCACCAT CCCCCAGGAG AAGCGCCGGG ACGAGGAACT CATCTACCAC 
AAAGTGACGG CGGCTGAGTT GCAGACCTTG GCGCCCGCCA TCAACTGGCT GCCCTTCCTC 
AACACCATCT TCTACCCCGT GGAGATCAAT GAATCAGAGC CTATTGTCAT CTACGACAAA 
GAATACCTGA GCAAGGTCTC CACCCTCATC AACAGCACAG ACAAATGCCT GCTGAACAAC 
TACATGATCT GGAACCTGGT ACGGAAGACG AGCTCCTTCC TCGATCAGCG CTTCCAGGAC 
GCCGACGAGA AGTTCATGGA AGTCATGTAT GGGACCAAGA AGACGTGTCT TCCCCGCTGG 
AAGTTTTGTG TGAGTGATAC AGAGAACACC TTGGGCTTCG CCCTGGGCCC CATGTTCGTC 
AAAGCGACCT TCGCTGAGGA CAGCAAGAAC ATAGCCAGCG AGATCATCCT GGAGATCAAG 
AAGGCGTTTG AAGAGAGCCT GAGCACCCTG AAGTGGATGG ATGAAGATAC TCGGAAATCG 
GCCAAGGAAA AGGCGGACGC GATCTACAAC ATGATAGGCT ACCCCAACTT TATCATGGAC 
CCCAAGGAGC TGGACAAAGT GTTCAATGAC TACACCGCTG TGCCAGACCT CTACTTCGAG 
AACGCCATGC GGTTTTTCAA CTTCTCCTGG AGGGTCACTG CCGACCAGCT CCGGAAAGCG 
CCCAACAGAG ATCAGTGGAG CATGACCCCG CCCATGGTGA ACGCCTACTA CTCGCCCACC 
AAGAACGAGA TCGTGTTTCC GGCCGGAATC CTGCAGGCGC CATTCTACAC CCGCTCTTCA 
CCCAATGCCT TAAACTTCGG CGGCATCGGC GTCGTCGTGG GCCACGAGCT GACTCATGCT 
TTTGATGATC AAGGCCGAGA GTACGACAAG GATGGGAACC TCCGGCCCTG GTGGAAGAAC 
TCGTCCGTGG AGGCGTTCAA GCAGCAGACC GCGTGCATGG TGGAGCAGTA CGGCAACTAT 
AGCGTGAACG GGGAGCCGGT GAACGGCCGG CACACCCTCG GCGAAAACAT CGCCGACAAC 
GGGGGCCTCA AGGCGGCCTA TCGGGCCTAC CAGAACTGGG TCAAGAAGAA TGGGGCTGAG 
CAGACACTGC CCACCCTGGG TCTCACCAAC AACCAGCTCT TCTTCCTGAG TTTTGGACAG 
GTCTGGTGTT CCGTCCGCAC CCCCGAGAGT TCGCACGAAG GTCTCATCAC CGATCCCCAC 
AGCCCCTCCC GCTTCCGGGT CATCGGCTCC ATCTCCAACT CCAAGGAGTT CTCGGAACAC 
TTCCACTGCC CGCCCGGCTC ACCCATGAAC CCGCATCACA AGTGTGAAGT CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_005203070.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203127.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa16887
Clone ID Related Accession (Same CDS sequence)	XM_005203070.3 , XM_005203070.4
Accession Version	XM_005203070.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCC TGTGGCCGCC GCGTCCGGTG GCCGCGGCGC TGTCGGCGCT GGGGATGTCT 
ACCTACAAGC GGGCCACGCT GGACGAGGAG GACCTGGTGG ACTCGCTGTC CGAGAGCGAC 
GTGTACCCCA ACCACCTGCA GGTGAACTTC CGAGGCCCCC GGAACGGCCA GAGATGCTGG 
GCCGCCAGGA CCCCGGTGGA GAAGCGGCTG GTGGTGCTGG TGGCGCTCCT GGCGGCGGCA 
TTGGTGGCCT GTTTGGCAGT ACTGGGCATC CAATACCAGA CAAGAACGCC CTCGGTGTGC 
CTAAGTGAGG CCTGCATCTC GGTGACCAGC TCCATCTTGA GTTCCATGGA CCCCACGGTG 
GACCCCTGCC AGGACTTCTT CACCTATGCC TGTGGCGGCT GGATCAAAGC CAACCCCGTG 
CCGGATGGCC ACTCGCGCTG GGGGACCTTC AGCAACCTCT GGGAACACAA CCAAGCCATC 
ATCAAGCACC TCCTTGAAAA CTCCACGGCC AGCGTGAGCG AGGCAGAGAG GAAGGCCCAG 
GTGTACTACC GAGCCTGCAT GAACGAAACC AGGATTGAGG AGCTCAAGGC CAAACCCCTG 
ATGGAGCTCA TTGAGAAGCT CGGCGGCTGG AACATCACGG GGCCCTGGGA CAAGGACAAC 
TTCCAGGACA CCCTGCAGGT GGTCACATCC CACTACCACA CCTCCCCCTT CTTCTCCGTC 
TACGTCAGTG CCGACTCCAA GAATTCCAAC AGCAACGTGA TCCAAGTGGA CCAGTCTGGC 
CTGGGCTTAC CCTCAAGAGA TTATTACCTG AACAAAACCG AGAATGAGAA GGTGCTGACG 
GGATACCTGA ACTACATGGT CCAGCTGGGG AAGCTGCTGG GAGGAGGGGC CGAGGACACC 
ATCCGGCCCC AGATGCAGCA GATCCTGGAC TTTGAGACGG CGCTGGCCAA CATCACCATC 
CCCCAGGAGA AGCGCCGGGA CGAGGAACTC ATCTACCACA AAGTGACGGC GGCTGAGTTG 
CAGACCTTGG CGCCCGCCAT CAACTGGCTG CCCTTCCTCA ACACCATCTT CTACCCCGTG 
GAGATCAATG AATCAGAGCC TATTGTCATC TACGACAAAG AATACCTGAG CAAGGTCTCC 
ACCCTCATCA ACAGCACAGA CAAATGCCTG CTGAACAACT ACATGATCTG GAACCTGGTA 
CGGAAGACGA GCTCCTTCCT CGATCAGCGC TTCCAGGACG CCGACGAGAA GTTCATGGAA 
GTCATGTATG GGACCAAGAA GACGTGTCTT CCCCGCTGGA AGTTTTGTGT GAGTGATACA 
GAGAACACCT TGGGCTTCGC CCTGGGCCCC ATGTTCGTCA AAGCGACCTT CGCTGAGGAC 
AGCAAGAACA TAGCCAGCGA GATCATCCTG GAGATCAAGA AGGCGTTTGA AGAGAGCCTG 
AGCACCCTGA AGTGGATGGA TGAAGATACT CGGAAATCGG CCAAGGAAAA GGCGGACGCG 
ATCTACAACA TGATAGGCTA CCCCAACTTT ATCATGGACC CCAAGGAGCT GGACAAAGTG 
TTCAATGACT ACACCGCTGT GCCAGACCTC TACTTCGAGA ACGCCATGCG GTTTTTCAAC 
TTCTCCTGGA GGGTCACTGC CGACCAGCTC CGGAAAGCGC CCAACAGAGA TCAGTGGAGC 
ATGACCCCGC CCATGGTGAA CGCCTACTAC TCGCCCACCA AGAACGAGAT CGTGTTTCCG 
GCCGGAATCC TGCAGGCGCC ATTCTACACC CGCTCTTCAC CCAATGCCTT AAACTTCGGC 
GGCATCGGCG TCGTCGTGGG CCACGAGCTG ACTCATGCTT TTGATGATCA AGGCCGAGAG 
TACGACAAGG ATGGGAACCT CCGGCCCTGG TGGAAGAACT CGTCCGTGGA GGCGTTCAAG 
CAGCAGACCG CGTGCATGGT GGAGCAGTAC GGCAACTATA GCGTGAACGG GGAGCCGGTG 
AACGGCCGGC ACACCCTCGG CGAAAACATC GCCGACAACG GGGGCCTCAA GGCGGCCTAT 
CGGGCCTACC AGAACTGGGT CAAGAAGAAT GGGGCTGAGC AGACACTGCC CACCCTGGGT 
CTCACCAACA ACCAGCTCTT CTTCCTGAGT TTTGCACAGG TCTGGTGTTC CGTCCGCACC 
CCCGAGAGTT CGCACGAAGG TCTCATCACC GATCCCCACA GCCCCTCCCG CTTCCGGGTC 
ATCGGCTCCA TCTCCAACTC CAAGGAGTTC TCGGAACACT TCCACTGCCC GCCCGGCTCA 
CCCATGAACC CGCATCACAA GTGTGAAGTC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203127.1
CDS	50..2365
Translation	MRSLWPPRPVAAALSALGMSTYKRATLDEEDLVDSLSESDVYPNHLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_005203070.3
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203070.3

ORF Insert Sequence:

ATGCGGAGCC TGTGGCCGCC GCGTCCGGTG GCCGCGGCGC TGTCGGCGCT GGGGATGTCT 
ACCTACAAGC GGGCCACGCT GGACGAGGAG GACCTGGTGG ACTCGCTGTC CGAGAGCGAC 
GTGTACCCCA ACCACCTGCA GGTGAACTTC CGAGGCCCCC GGAACGGCCA GAGATGCTGG 
GCCGCCAGGA CCCCGGTGGA GAAGCGGCTG GTGGTGCTGG TGGCGCTCCT GGCGGCGGCA 
TTGGTGGCCT GTTTGGCAGT ACTGGGCATC CAATACCAGA CAAGAACGCC CTCGGTGTGC 
CTAAGTGAGG CCTGCATCTC GGTGACCAGC TCCATCTTGA GTTCCATGGA CCCCACGGTG 
GACCCCTGCC AGGACTTCTT CACCTATGCC TGTGGCGGCT GGATCAAAGC CAACCCCGTG 
CCGGATGGCC ACTCGCGCTG GGGGACCTTC AGCAACCTCT GGGAACACAA CCAAGCCATC 
ATCAAGCACC TCCTTGAAAA CTCCACGGCC AGCGTGAGCG AGGCAGAGAG GAAGGCCCAG 
GTGTACTACC GAGCCTGCAT GAACGAAACC AGGATTGAGG AGCTCAAGGC CAAACCCCTG 
ATGGAGCTCA TTGAGAAGCT CGGCGGCTGG AACATCACGG GGCCCTGGGA CAAGGACAAC 
TTCCAGGACA CCCTGCAGGT GGTCACATCC CACTACCACA CCTCCCCCTT CTTCTCCGTC 
TACGTCAGTG CCGACTCCAA GAATTCCAAC AGCAACGTGA TCCAAGTGGA CCAGTCTGGC 
CTGGGCTTAC CCTCAAGAGA TTATTACCTG AACAAAACCG AGAATGAGAA GGTGCTGACG 
GGATACCTGA ACTACATGGT CCAGCTGGGG AAGCTGCTGG GAGGAGGGGC CGAGGACACC 
ATCCGGCCCC AGATGCAGCA GATCCTGGAC TTTGAGACGG CGCTGGCCAA CATCACCATC 
CCCCAGGAGA AGCGCCGGGA CGAGGAACTC ATCTACCACA AAGTGACGGC GGCTGAGTTG 
CAGACCTTGG CGCCCGCCAT CAACTGGCTG CCCTTCCTCA ACACCATCTT CTACCCCGTG 
GAGATCAATG AATCAGAGCC TATTGTCATC TACGACAAAG AATACCTGAG CAAGGTCTCC 
ACCCTCATCA ACAGCACAGA CAAATGCCTG CTGAACAACT ACATGATCTG GAACCTGGTA 
CGGAAGACGA GCTCCTTCCT CGATCAGCGC TTCCAGGACG CCGACGAGAA GTTCATGGAA 
GTCATGTATG GGACCAAGAA GACGTGTCTT CCCCGCTGGA AGTTTTGTGT GAGTGATACA 
GAGAACACCT TGGGCTTCGC CCTGGGCCCC ATGTTCGTCA AAGCGACCTT CGCTGAGGAC 
AGCAAGAACA TAGCCAGCGA GATCATCCTG GAGATCAAGA AGGCGTTTGA AGAGAGCCTG 
AGCACCCTGA AGTGGATGGA TGAAGATACT CGGAAATCGG CCAAGGAAAA GGCGGACGCG 
ATCTACAACA TGATAGGCTA CCCCAACTTT ATCATGGACC CCAAGGAGCT GGACAAAGTG 
TTCAATGACT ACACCGCTGT GCCAGACCTC TACTTCGAGA ACGCCATGCG GTTTTTCAAC 
TTCTCCTGGA GGGTCACTGC CGACCAGCTC CGGAAAGCGC CCAACAGAGA TCAGTGGAGC 
ATGACCCCGC CCATGGTGAA CGCCTACTAC TCGCCCACCA AGAACGAGAT CGTGTTTCCG 
GCCGGAATCC TGCAGGCGCC ATTCTACACC CGCTCTTCAC CCAATGCCTT AAACTTCGGC 
GGCATCGGCG TCGTCGTGGG CCACGAGCTG ACTCATGCTT TTGATGATCA AGGCCGAGAG 
TACGACAAGG ATGGGAACCT CCGGCCCTGG TGGAAGAACT CGTCCGTGGA GGCGTTCAAG 
CAGCAGACCG CGTGCATGGT GGAGCAGTAC GGCAACTATA GCGTGAACGG GGAGCCGGTG 
AACGGCCGGC ACACCCTCGG CGAAAACATC GCCGACAACG GGGGCCTCAA GGCGGCCTAT 
CGGGCCTACC AGAACTGGGT CAAGAAGAAT GGGGCTGAGC AGACACTGCC CACCCTGGGT 
CTCACCAACA ACCAGCTCTT CTTCCTGAGT TTTGCACAGG TCTGGTGTTC CGTCCGCACC 
CCCGAGAGTT CGCACGAAGG TCTCATCACC GATCCCCACA GCCCCTCCCG CTTCCGGGTC 
ATCGGCTCCA TCTCCAACTC CAAGGAGTTC TCGGAACACT TCCACTGCCC GCCCGGCTCA 
CCCATGAACC CGCATCACAA GTGTGAAGTC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_005203070.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203127.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa16887
Clone ID Related Accession (Same CDS sequence)	XM_005203070.3 , XM_005203070.4
Accession Version	XM_005203070.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203070.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCC TGTGGCCGCC GCGTCCGGTG GCCGCGGCGC TGTCGGCGCT GGGGATGTCT 
ACCTACAAGC GGGCCACGCT GGACGAGGAG GACCTGGTGG ACTCGCTGTC CGAGAGCGAC 
GTGTACCCCA ACCACCTGCA GGTGAACTTC CGAGGCCCCC GGAACGGCCA GAGATGCTGG 
GCCGCCAGGA CCCCGGTGGA GAAGCGGCTG GTGGTGCTGG TGGCGCTCCT GGCGGCGGCA 
TTGGTGGCCT GTTTGGCAGT ACTGGGCATC CAATACCAGA CAAGAACGCC CTCGGTGTGC 
CTAAGTGAGG CCTGCATCTC GGTGACCAGC TCCATCTTGA GTTCCATGGA CCCCACGGTG 
GACCCCTGCC AGGACTTCTT CACCTATGCC TGTGGCGGCT GGATCAAAGC CAACCCCGTG 
CCGGATGGCC ACTCGCGCTG GGGGACCTTC AGCAACCTCT GGGAACACAA CCAAGCCATC 
ATCAAGCACC TCCTTGAAAA CTCCACGGCC AGCGTGAGCG AGGCAGAGAG GAAGGCCCAG 
GTGTACTACC GAGCCTGCAT GAACGAAACC AGGATTGAGG AGCTCAAGGC CAAACCCCTG 
ATGGAGCTCA TTGAGAAGCT CGGCGGCTGG AACATCACGG GGCCCTGGGA CAAGGACAAC 
TTCCAGGACA CCCTGCAGGT GGTCACATCC CACTACCACA CCTCCCCCTT CTTCTCCGTC 
TACGTCAGTG CCGACTCCAA GAATTCCAAC AGCAACGTGA TCCAAGTGGA CCAGTCTGGC 
CTGGGCTTAC CCTCAAGAGA TTATTACCTG AACAAAACCG AGAATGAGAA GGTGCTGACG 
GGATACCTGA ACTACATGGT CCAGCTGGGG AAGCTGCTGG GAGGAGGGGC CGAGGACACC 
ATCCGGCCCC AGATGCAGCA GATCCTGGAC TTTGAGACGG CGCTGGCCAA CATCACCATC 
CCCCAGGAGA AGCGCCGGGA CGAGGAACTC ATCTACCACA AAGTGACGGC GGCTGAGTTG 
CAGACCTTGG CGCCCGCCAT CAACTGGCTG CCCTTCCTCA ACACCATCTT CTACCCCGTG 
GAGATCAATG AATCAGAGCC TATTGTCATC TACGACAAAG AATACCTGAG CAAGGTCTCC 
ACCCTCATCA ACAGCACAGA CAAATGCCTG CTGAACAACT ACATGATCTG GAACCTGGTA 
CGGAAGACGA GCTCCTTCCT CGATCAGCGC TTCCAGGACG CCGACGAGAA GTTCATGGAA 
GTCATGTATG GGACCAAGAA GACGTGTCTT CCCCGCTGGA AGTTTTGTGT GAGTGATACA 
GAGAACACCT TGGGCTTCGC CCTGGGCCCC ATGTTCGTCA AAGCGACCTT CGCTGAGGAC 
AGCAAGAACA TAGCCAGCGA GATCATCCTG GAGATCAAGA AGGCGTTTGA AGAGAGCCTG 
AGCACCCTGA AGTGGATGGA TGAAGATACT CGGAAATCGG CCAAGGAAAA GGCGGACGCG 
ATCTACAACA TGATAGGCTA CCCCAACTTT ATCATGGACC CCAAGGAGCT GGACAAAGTG 
TTCAATGACT ACACCGCTGT GCCAGACCTC TACTTCGAGA ACGCCATGCG GTTTTTCAAC 
TTCTCCTGGA GGGTCACTGC CGACCAGCTC CGGAAAGCGC CCAACAGAGA TCAGTGGAGC 
ATGACCCCGC CCATGGTGAA CGCCTACTAC TCGCCCACCA AGAACGAGAT CGTGTTTCCG 
GCCGGAATCC TGCAGGCGCC ATTCTACACC CGCTCTTCAC CCAATGCCTT AAACTTCGGC 
GGCATCGGCG TCGTCGTGGG CCACGAGCTG ACTCATGCTT TTGATGATCA AGGCCGAGAG 
TACGACAAGG ATGGGAACCT CCGGCCCTGG TGGAAGAACT CGTCCGTGGA GGCGTTCAAG 
CAGCAGACCG CGTGCATGGT GGAGCAGTAC GGCAACTATA GCGTGAACGG GGAGCCGGTG 
AACGGCCGGC ACACCCTCGG CGAAAACATC GCCGACAACG GGGGCCTCAA GGCGGCCTAT 
CGGGCCTACC AGAACTGGGT CAAGAAGAAT GGGGCTGAGC AGACACTGCC CACCCTGGGT 
CTCACCAACA ACCAGCTCTT CTTCCTGAGT TTTGCACAGG TCTGGTGTTC CGTCCGCACC 
CCCGAGAGTT CGCACGAAGG TCTCATCACC GATCCCCACA GCCCCTCCCG CTTCCGGGTC 
ATCGGCTCCA TCTCCAACTC CAAGGAGTTC TCGGAACACT TCCACTGCCC GCCCGGCTCA 
CCCATGAACC CGCATCACAA GTGTGAAGTC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203127.1
CDS	53..2368
Translation	MRSLWPPRPVAAALSALGMSTYKRATLDEEDLVDSLSESDVYPNHLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_005203070.4
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203070.4

ORF Insert Sequence:

ATGCGGAGCC TGTGGCCGCC GCGTCCGGTG GCCGCGGCGC TGTCGGCGCT GGGGATGTCT 
ACCTACAAGC GGGCCACGCT GGACGAGGAG GACCTGGTGG ACTCGCTGTC CGAGAGCGAC 
GTGTACCCCA ACCACCTGCA GGTGAACTTC CGAGGCCCCC GGAACGGCCA GAGATGCTGG 
GCCGCCAGGA CCCCGGTGGA GAAGCGGCTG GTGGTGCTGG TGGCGCTCCT GGCGGCGGCA 
TTGGTGGCCT GTTTGGCAGT ACTGGGCATC CAATACCAGA CAAGAACGCC CTCGGTGTGC 
CTAAGTGAGG CCTGCATCTC GGTGACCAGC TCCATCTTGA GTTCCATGGA CCCCACGGTG 
GACCCCTGCC AGGACTTCTT CACCTATGCC TGTGGCGGCT GGATCAAAGC CAACCCCGTG 
CCGGATGGCC ACTCGCGCTG GGGGACCTTC AGCAACCTCT GGGAACACAA CCAAGCCATC 
ATCAAGCACC TCCTTGAAAA CTCCACGGCC AGCGTGAGCG AGGCAGAGAG GAAGGCCCAG 
GTGTACTACC GAGCCTGCAT GAACGAAACC AGGATTGAGG AGCTCAAGGC CAAACCCCTG 
ATGGAGCTCA TTGAGAAGCT CGGCGGCTGG AACATCACGG GGCCCTGGGA CAAGGACAAC 
TTCCAGGACA CCCTGCAGGT GGTCACATCC CACTACCACA CCTCCCCCTT CTTCTCCGTC 
TACGTCAGTG CCGACTCCAA GAATTCCAAC AGCAACGTGA TCCAAGTGGA CCAGTCTGGC 
CTGGGCTTAC CCTCAAGAGA TTATTACCTG AACAAAACCG AGAATGAGAA GGTGCTGACG 
GGATACCTGA ACTACATGGT CCAGCTGGGG AAGCTGCTGG GAGGAGGGGC CGAGGACACC 
ATCCGGCCCC AGATGCAGCA GATCCTGGAC TTTGAGACGG CGCTGGCCAA CATCACCATC 
CCCCAGGAGA AGCGCCGGGA CGAGGAACTC ATCTACCACA AAGTGACGGC GGCTGAGTTG 
CAGACCTTGG CGCCCGCCAT CAACTGGCTG CCCTTCCTCA ACACCATCTT CTACCCCGTG 
GAGATCAATG AATCAGAGCC TATTGTCATC TACGACAAAG AATACCTGAG CAAGGTCTCC 
ACCCTCATCA ACAGCACAGA CAAATGCCTG CTGAACAACT ACATGATCTG GAACCTGGTA 
CGGAAGACGA GCTCCTTCCT CGATCAGCGC TTCCAGGACG CCGACGAGAA GTTCATGGAA 
GTCATGTATG GGACCAAGAA GACGTGTCTT CCCCGCTGGA AGTTTTGTGT GAGTGATACA 
GAGAACACCT TGGGCTTCGC CCTGGGCCCC ATGTTCGTCA AAGCGACCTT CGCTGAGGAC 
AGCAAGAACA TAGCCAGCGA GATCATCCTG GAGATCAAGA AGGCGTTTGA AGAGAGCCTG 
AGCACCCTGA AGTGGATGGA TGAAGATACT CGGAAATCGG CCAAGGAAAA GGCGGACGCG 
ATCTACAACA TGATAGGCTA CCCCAACTTT ATCATGGACC CCAAGGAGCT GGACAAAGTG 
TTCAATGACT ACACCGCTGT GCCAGACCTC TACTTCGAGA ACGCCATGCG GTTTTTCAAC 
TTCTCCTGGA GGGTCACTGC CGACCAGCTC CGGAAAGCGC CCAACAGAGA TCAGTGGAGC 
ATGACCCCGC CCATGGTGAA CGCCTACTAC TCGCCCACCA AGAACGAGAT CGTGTTTCCG 
GCCGGAATCC TGCAGGCGCC ATTCTACACC CGCTCTTCAC CCAATGCCTT AAACTTCGGC 
GGCATCGGCG TCGTCGTGGG CCACGAGCTG ACTCATGCTT TTGATGATCA AGGCCGAGAG 
TACGACAAGG ATGGGAACCT CCGGCCCTGG TGGAAGAACT CGTCCGTGGA GGCGTTCAAG 
CAGCAGACCG CGTGCATGGT GGAGCAGTAC GGCAACTATA GCGTGAACGG GGAGCCGGTG 
AACGGCCGGC ACACCCTCGG CGAAAACATC GCCGACAACG GGGGCCTCAA GGCGGCCTAT 
CGGGCCTACC AGAACTGGGT CAAGAAGAAT GGGGCTGAGC AGACACTGCC CACCCTGGGT 
CTCACCAACA ACCAGCTCTT CTTCCTGAGT TTTGCACAGG TCTGGTGTTC CGTCCGCACC 
CCCGAGAGTT CGCACGAAGG TCTCATCACC GATCCCCACA GCCCCTCCCG CTTCCGGGTC 
ATCGGCTCCA TCTCCAACTC CAAGGAGTTC TCGGAACACT TCCACTGCCC GCCCGGCTCA 
CCCATGAACC CGCATCACAA GTGTGAAGTC TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_005203072.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203129.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X4, mRNA.

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CloneID	OBa16889
Clone ID Related Accession (Same CDS sequence)	XM_005203072.3 , XM_005203072.4
Accession Version	XM_005203072.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTA CCTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTGTCC 
GAGAGCGACG TGTACCCCAA CCACCTGCAG GTGAACTTCC GAGGCCCCCG GAACGGCCAG 
AGATGCTGGG CCGCCAGGAC CCCGGTGGAG AAGCGGCTGG TGGTGCTGGT GGCGCTCCTG 
GCGGCGGCAT TGGTGGCCTG TTTGGCAGTA CTGGGCATCC AATACCAGAC AAGAACGCCC 
TCGGTGTGCC TAAGTGAGGC CTGCATCTCG GTGACCAGCT CCATCTTGAG TTCCATGGAC 
CCCACGGTGG ACCCCTGCCA GGACTTCTTC ACCTATGCCT GTGGCGGCTG GATCAAAGCC 
AACCCCGTGC CGGATGGCCA CTCGCGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCCATCA TCAAGCACCT CCTTGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGG 
AAGGCCCAGG TGTACTACCG AGCCTGCATG AACGAAACCA GGATTGAGGA GCTCAAGGCC 
AAACCCCTGA TGGAGCTCAT TGAGAAGCTC GGCGGCTGGA ACATCACGGG GCCCTGGGAC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACATCCC ACTACCACAC CTCCCCCTTC 
TTCTCCGTCT ACGTCAGTGC CGACTCCAAG AATTCCAACA GCAACGTGAT CCAAGTGGAC 
CAGTCTGGCC TGGGCTTACC CTCAAGAGAT TATTACCTGA ACAAAACCGA GAATGAGAAG 
GTGCTGACGG GATACCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG AGGAGGGGCC 
GAGGACACCA TCCGGCCCCA GATGCAGCAG ATCCTGGACT TTGAGACGGC GCTGGCCAAC 
ATCACCATCC CCCAGGAGAA GCGCCGGGAC GAGGAACTCA TCTACCACAA AGTGACGGCG 
GCTGAGTTGC AGACCTTGGC GCCCGCCATC AACTGGCTGC CCTTCCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCAGAGCCT ATTGTCATCT ACGACAAAGA ATACCTGAGC 
AAGGTCTCCA CCCTCATCAA CAGCACAGAC AAATGCCTGC TGAACAACTA CATGATCTGG 
AACCTGGTAC GGAAGACGAG CTCCTTCCTC GATCAGCGCT TCCAGGACGC CGACGAGAAG 
TTCATGGAAG TCATGTATGG GACCAAGAAG ACGTGTCTTC CCCGCTGGAA GTTTTGTGTG 
AGTGATACAG AGAACACCTT GGGCTTCGCC CTGGGCCCCA TGTTCGTCAA AGCGACCTTC 
GCTGAGGACA GCAAGAACAT AGCCAGCGAG ATCATCCTGG AGATCAAGAA GGCGTTTGAA 
GAGAGCCTGA GCACCCTGAA GTGGATGGAT GAAGATACTC GGAAATCGGC CAAGGAAAAG 
GCGGACGCGA TCTACAACAT GATAGGCTAC CCCAACTTTA TCATGGACCC CAAGGAGCTG 
GACAAAGTGT TCAATGACTA CACCGCTGTG CCAGACCTCT ACTTCGAGAA CGCCATGCGG 
TTTTTCAACT TCTCCTGGAG GGTCACTGCC GACCAGCTCC GGAAAGCGCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAACGAGATC 
GTGTTTCCGG CCGGAATCCT GCAGGCGCCA TTCTACACCC GCTCTTCACC CAATGCCTTA 
AACTTCGGCG GCATCGGCGT CGTCGTGGGC CACGAGCTGA CTCATGCTTT TGATGATCAA 
GGCCGAGAGT ACGACAAGGA TGGGAACCTC CGGCCCTGGT GGAAGAACTC GTCCGTGGAG 
GCGTTCAAGC AGCAGACCGC GTGCATGGTG GAGCAGTACG GCAACTATAG CGTGAACGGG 
GAGCCGGTGA ACGGCCGGCA CACCCTCGGC GAAAACATCG CCGACAACGG GGGCCTCAAG 
GCGGCCTATC GGGCCTACCA GAACTGGGTC AAGAAGAATG GGGCTGAGCA GACACTGCCC 
ACCCTGGGTC TCACCAACAA CCAGCTCTTC TTCCTGAGTT TTGCACAGGT CTGGTGTTCC 
GTCCGCACCC CCGAGAGTTC GCACGAAGGT CTCATCACCG ATCCCCACAG CCCCTCCCGC 
TTCCGGGTCA TCGGCTCCAT CTCCAACTCC AAGGAGTTCT CGGAACACTT CCACTGCCCG 
CCCGGCTCAC CCATGAACCC GCATCACAAG TGTGAAGTCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203129.1
CDS	110..2374
Translation	MMSTYKRATLDEEDLVDSLSESDVYPNHLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_005203072.3
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203072.3

ORF Insert Sequence:

ATGATGTCTA CCTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTGTCC 
GAGAGCGACG TGTACCCCAA CCACCTGCAG GTGAACTTCC GAGGCCCCCG GAACGGCCAG 
AGATGCTGGG CCGCCAGGAC CCCGGTGGAG AAGCGGCTGG TGGTGCTGGT GGCGCTCCTG 
GCGGCGGCAT TGGTGGCCTG TTTGGCAGTA CTGGGCATCC AATACCAGAC AAGAACGCCC 
TCGGTGTGCC TAAGTGAGGC CTGCATCTCG GTGACCAGCT CCATCTTGAG TTCCATGGAC 
CCCACGGTGG ACCCCTGCCA GGACTTCTTC ACCTATGCCT GTGGCGGCTG GATCAAAGCC 
AACCCCGTGC CGGATGGCCA CTCGCGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCCATCA TCAAGCACCT CCTTGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGG 
AAGGCCCAGG TGTACTACCG AGCCTGCATG AACGAAACCA GGATTGAGGA GCTCAAGGCC 
AAACCCCTGA TGGAGCTCAT TGAGAAGCTC GGCGGCTGGA ACATCACGGG GCCCTGGGAC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACATCCC ACTACCACAC CTCCCCCTTC 
TTCTCCGTCT ACGTCAGTGC CGACTCCAAG AATTCCAACA GCAACGTGAT CCAAGTGGAC 
CAGTCTGGCC TGGGCTTACC CTCAAGAGAT TATTACCTGA ACAAAACCGA GAATGAGAAG 
GTGCTGACGG GATACCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG AGGAGGGGCC 
GAGGACACCA TCCGGCCCCA GATGCAGCAG ATCCTGGACT TTGAGACGGC GCTGGCCAAC 
ATCACCATCC CCCAGGAGAA GCGCCGGGAC GAGGAACTCA TCTACCACAA AGTGACGGCG 
GCTGAGTTGC AGACCTTGGC GCCCGCCATC AACTGGCTGC CCTTCCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCAGAGCCT ATTGTCATCT ACGACAAAGA ATACCTGAGC 
AAGGTCTCCA CCCTCATCAA CAGCACAGAC AAATGCCTGC TGAACAACTA CATGATCTGG 
AACCTGGTAC GGAAGACGAG CTCCTTCCTC GATCAGCGCT TCCAGGACGC CGACGAGAAG 
TTCATGGAAG TCATGTATGG GACCAAGAAG ACGTGTCTTC CCCGCTGGAA GTTTTGTGTG 
AGTGATACAG AGAACACCTT GGGCTTCGCC CTGGGCCCCA TGTTCGTCAA AGCGACCTTC 
GCTGAGGACA GCAAGAACAT AGCCAGCGAG ATCATCCTGG AGATCAAGAA GGCGTTTGAA 
GAGAGCCTGA GCACCCTGAA GTGGATGGAT GAAGATACTC GGAAATCGGC CAAGGAAAAG 
GCGGACGCGA TCTACAACAT GATAGGCTAC CCCAACTTTA TCATGGACCC CAAGGAGCTG 
GACAAAGTGT TCAATGACTA CACCGCTGTG CCAGACCTCT ACTTCGAGAA CGCCATGCGG 
TTTTTCAACT TCTCCTGGAG GGTCACTGCC GACCAGCTCC GGAAAGCGCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAACGAGATC 
GTGTTTCCGG CCGGAATCCT GCAGGCGCCA TTCTACACCC GCTCTTCACC CAATGCCTTA 
AACTTCGGCG GCATCGGCGT CGTCGTGGGC CACGAGCTGA CTCATGCTTT TGATGATCAA 
GGCCGAGAGT ACGACAAGGA TGGGAACCTC CGGCCCTGGT GGAAGAACTC GTCCGTGGAG 
GCGTTCAAGC AGCAGACCGC GTGCATGGTG GAGCAGTACG GCAACTATAG CGTGAACGGG 
GAGCCGGTGA ACGGCCGGCA CACCCTCGGC GAAAACATCG CCGACAACGG GGGCCTCAAG 
GCGGCCTATC GGGCCTACCA GAACTGGGTC AAGAAGAATG GGGCTGAGCA GACACTGCCC 
ACCCTGGGTC TCACCAACAA CCAGCTCTTC TTCCTGAGTT TTGCACAGGT CTGGTGTTCC 
GTCCGCACCC CCGAGAGTTC GCACGAAGGT CTCATCACCG ATCCCCACAG CCCCTCCCGC 
TTCCGGGTCA TCGGCTCCAT CTCCAACTCC AAGGAGTTCT CGGAACACTT CCACTGCCCG 
CCCGGCTCAC CCATGAACCC GCATCACAAG TGTGAAGTCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_005203072.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203129.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa16889
Clone ID Related Accession (Same CDS sequence)	XM_005203072.3 , XM_005203072.4
Accession Version	XM_005203072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203072.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTA CCTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTGTCC 
GAGAGCGACG TGTACCCCAA CCACCTGCAG GTGAACTTCC GAGGCCCCCG GAACGGCCAG 
AGATGCTGGG CCGCCAGGAC CCCGGTGGAG AAGCGGCTGG TGGTGCTGGT GGCGCTCCTG 
GCGGCGGCAT TGGTGGCCTG TTTGGCAGTA CTGGGCATCC AATACCAGAC AAGAACGCCC 
TCGGTGTGCC TAAGTGAGGC CTGCATCTCG GTGACCAGCT CCATCTTGAG TTCCATGGAC 
CCCACGGTGG ACCCCTGCCA GGACTTCTTC ACCTATGCCT GTGGCGGCTG GATCAAAGCC 
AACCCCGTGC CGGATGGCCA CTCGCGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCCATCA TCAAGCACCT CCTTGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGG 
AAGGCCCAGG TGTACTACCG AGCCTGCATG AACGAAACCA GGATTGAGGA GCTCAAGGCC 
AAACCCCTGA TGGAGCTCAT TGAGAAGCTC GGCGGCTGGA ACATCACGGG GCCCTGGGAC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACATCCC ACTACCACAC CTCCCCCTTC 
TTCTCCGTCT ACGTCAGTGC CGACTCCAAG AATTCCAACA GCAACGTGAT CCAAGTGGAC 
CAGTCTGGCC TGGGCTTACC CTCAAGAGAT TATTACCTGA ACAAAACCGA GAATGAGAAG 
GTGCTGACGG GATACCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG AGGAGGGGCC 
GAGGACACCA TCCGGCCCCA GATGCAGCAG ATCCTGGACT TTGAGACGGC GCTGGCCAAC 
ATCACCATCC CCCAGGAGAA GCGCCGGGAC GAGGAACTCA TCTACCACAA AGTGACGGCG 
GCTGAGTTGC AGACCTTGGC GCCCGCCATC AACTGGCTGC CCTTCCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCAGAGCCT ATTGTCATCT ACGACAAAGA ATACCTGAGC 
AAGGTCTCCA CCCTCATCAA CAGCACAGAC AAATGCCTGC TGAACAACTA CATGATCTGG 
AACCTGGTAC GGAAGACGAG CTCCTTCCTC GATCAGCGCT TCCAGGACGC CGACGAGAAG 
TTCATGGAAG TCATGTATGG GACCAAGAAG ACGTGTCTTC CCCGCTGGAA GTTTTGTGTG 
AGTGATACAG AGAACACCTT GGGCTTCGCC CTGGGCCCCA TGTTCGTCAA AGCGACCTTC 
GCTGAGGACA GCAAGAACAT AGCCAGCGAG ATCATCCTGG AGATCAAGAA GGCGTTTGAA 
GAGAGCCTGA GCACCCTGAA GTGGATGGAT GAAGATACTC GGAAATCGGC CAAGGAAAAG 
GCGGACGCGA TCTACAACAT GATAGGCTAC CCCAACTTTA TCATGGACCC CAAGGAGCTG 
GACAAAGTGT TCAATGACTA CACCGCTGTG CCAGACCTCT ACTTCGAGAA CGCCATGCGG 
TTTTTCAACT TCTCCTGGAG GGTCACTGCC GACCAGCTCC GGAAAGCGCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAACGAGATC 
GTGTTTCCGG CCGGAATCCT GCAGGCGCCA TTCTACACCC GCTCTTCACC CAATGCCTTA 
AACTTCGGCG GCATCGGCGT CGTCGTGGGC CACGAGCTGA CTCATGCTTT TGATGATCAA 
GGCCGAGAGT ACGACAAGGA TGGGAACCTC CGGCCCTGGT GGAAGAACTC GTCCGTGGAG 
GCGTTCAAGC AGCAGACCGC GTGCATGGTG GAGCAGTACG GCAACTATAG CGTGAACGGG 
GAGCCGGTGA ACGGCCGGCA CACCCTCGGC GAAAACATCG CCGACAACGG GGGCCTCAAG 
GCGGCCTATC GGGCCTACCA GAACTGGGTC AAGAAGAATG GGGCTGAGCA GACACTGCCC 
ACCCTGGGTC TCACCAACAA CCAGCTCTTC TTCCTGAGTT TTGCACAGGT CTGGTGTTCC 
GTCCGCACCC CCGAGAGTTC GCACGAAGGT CTCATCACCG ATCCCCACAG CCCCTCCCGC 
TTCCGGGTCA TCGGCTCCAT CTCCAACTCC AAGGAGTTCT CGGAACACTT CCACTGCCCG 
CCCGGCTCAC CCATGAACCC GCATCACAAG TGTGAAGTCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203129.1
CDS	137..2401
Translation	MMSTYKRATLDEEDLVDSLSESDVYPNHLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_005203072.4
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203072.4

ORF Insert Sequence:

ATGATGTCTA CCTACAAGCG GGCCACGCTG GACGAGGAGG ACCTGGTGGA CTCGCTGTCC 
GAGAGCGACG TGTACCCCAA CCACCTGCAG GTGAACTTCC GAGGCCCCCG GAACGGCCAG 
AGATGCTGGG CCGCCAGGAC CCCGGTGGAG AAGCGGCTGG TGGTGCTGGT GGCGCTCCTG 
GCGGCGGCAT TGGTGGCCTG TTTGGCAGTA CTGGGCATCC AATACCAGAC AAGAACGCCC 
TCGGTGTGCC TAAGTGAGGC CTGCATCTCG GTGACCAGCT CCATCTTGAG TTCCATGGAC 
CCCACGGTGG ACCCCTGCCA GGACTTCTTC ACCTATGCCT GTGGCGGCTG GATCAAAGCC 
AACCCCGTGC CGGATGGCCA CTCGCGCTGG GGGACCTTCA GCAACCTCTG GGAACACAAC 
CAAGCCATCA TCAAGCACCT CCTTGAAAAC TCCACGGCCA GCGTGAGCGA GGCAGAGAGG 
AAGGCCCAGG TGTACTACCG AGCCTGCATG AACGAAACCA GGATTGAGGA GCTCAAGGCC 
AAACCCCTGA TGGAGCTCAT TGAGAAGCTC GGCGGCTGGA ACATCACGGG GCCCTGGGAC 
AAGGACAACT TCCAGGACAC CCTGCAGGTG GTCACATCCC ACTACCACAC CTCCCCCTTC 
TTCTCCGTCT ACGTCAGTGC CGACTCCAAG AATTCCAACA GCAACGTGAT CCAAGTGGAC 
CAGTCTGGCC TGGGCTTACC CTCAAGAGAT TATTACCTGA ACAAAACCGA GAATGAGAAG 
GTGCTGACGG GATACCTGAA CTACATGGTC CAGCTGGGGA AGCTGCTGGG AGGAGGGGCC 
GAGGACACCA TCCGGCCCCA GATGCAGCAG ATCCTGGACT TTGAGACGGC GCTGGCCAAC 
ATCACCATCC CCCAGGAGAA GCGCCGGGAC GAGGAACTCA TCTACCACAA AGTGACGGCG 
GCTGAGTTGC AGACCTTGGC GCCCGCCATC AACTGGCTGC CCTTCCTCAA CACCATCTTC 
TACCCCGTGG AGATCAATGA ATCAGAGCCT ATTGTCATCT ACGACAAAGA ATACCTGAGC 
AAGGTCTCCA CCCTCATCAA CAGCACAGAC AAATGCCTGC TGAACAACTA CATGATCTGG 
AACCTGGTAC GGAAGACGAG CTCCTTCCTC GATCAGCGCT TCCAGGACGC CGACGAGAAG 
TTCATGGAAG TCATGTATGG GACCAAGAAG ACGTGTCTTC CCCGCTGGAA GTTTTGTGTG 
AGTGATACAG AGAACACCTT GGGCTTCGCC CTGGGCCCCA TGTTCGTCAA AGCGACCTTC 
GCTGAGGACA GCAAGAACAT AGCCAGCGAG ATCATCCTGG AGATCAAGAA GGCGTTTGAA 
GAGAGCCTGA GCACCCTGAA GTGGATGGAT GAAGATACTC GGAAATCGGC CAAGGAAAAG 
GCGGACGCGA TCTACAACAT GATAGGCTAC CCCAACTTTA TCATGGACCC CAAGGAGCTG 
GACAAAGTGT TCAATGACTA CACCGCTGTG CCAGACCTCT ACTTCGAGAA CGCCATGCGG 
TTTTTCAACT TCTCCTGGAG GGTCACTGCC GACCAGCTCC GGAAAGCGCC CAACAGAGAT 
CAGTGGAGCA TGACCCCGCC CATGGTGAAC GCCTACTACT CGCCCACCAA GAACGAGATC 
GTGTTTCCGG CCGGAATCCT GCAGGCGCCA TTCTACACCC GCTCTTCACC CAATGCCTTA 
AACTTCGGCG GCATCGGCGT CGTCGTGGGC CACGAGCTGA CTCATGCTTT TGATGATCAA 
GGCCGAGAGT ACGACAAGGA TGGGAACCTC CGGCCCTGGT GGAAGAACTC GTCCGTGGAG 
GCGTTCAAGC AGCAGACCGC GTGCATGGTG GAGCAGTACG GCAACTATAG CGTGAACGGG 
GAGCCGGTGA ACGGCCGGCA CACCCTCGGC GAAAACATCG CCGACAACGG GGGCCTCAAG 
GCGGCCTATC GGGCCTACCA GAACTGGGTC AAGAAGAATG GGGCTGAGCA GACACTGCCC 
ACCCTGGGTC TCACCAACAA CCAGCTCTTC TTCCTGAGTT TTGCACAGGT CTGGTGTTCC 
GTCCGCACCC CCGAGAGTTC GCACGAAGGT CTCATCACCG ATCCCCACAG CCCCTCCCGC 
TTCCGGGTCA TCGGCTCCAT CTCCAACTCC AAGGAGTTCT CGGAACACTT CCACTGCCCG 
CCCGGCTCAC CCATGAACCC GCATCACAAG TGTGAAGTCT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_010802523.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800825.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa45074
Clone ID Related Accession (Same CDS sequence)	XM_010802523.2
Accession Version	XM_010802523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCC CTTGGCATTC CCCACTGACA TCTCCCGGGA CTCAATGCTC TGACCCGTGG 
GTGGGCTCTG GGGTCCATAC CTTTGGGCCC AGATCCAAGC TCGACCCATC TAGCCGTGTG 
AACTTCCGAG GCCCCCGGAA CGGCCAGAGA TGCTGGGCCG CCAGGACCCC GGTGGAGAAG 
CGGCTGGTGG TGCTGGTGGC GCTCCTGGCG GCGGCATTGG TGGCCTGTTT GGCAGTACTG 
GGCATCCAAT ACCAGACAAG AACGCCCTCG GTGTGCCTAA GTGAGGCCTG CATCTCGGTG 
ACCAGCTCCA TCTTGAGTTC CATGGACCCC ACGGTGGACC CCTGCCAGGA CTTCTTCACC 
TATGCCTGTG GCGGCTGGAT CAAAGCCAAC CCCGTGCCGG ATGGCCACTC GCGCTGGGGG 
ACCTTCAGCA ACCTCTGGGA ACACAACCAA GCCATCATCA AGCACCTCCT TGAAAACTCC 
ACGGCCAGCG TGAGCGAGGC AGAGAGGAAG GCCCAGGTGT ACTACCGAGC CTGCATGAAC 
GAAACCAGGA TTGAGGAGCT CAAGGCCAAA CCCCTGATGG AGCTCATTGA GAAGCTCGGC 
GGCTGGAACA TCACGGGGCC CTGGGACAAG GACAACTTCC AGGACACCCT GCAGGTGGTC 
ACATCCCACT ACCACACCTC CCCCTTCTTC TCCGTCTACG TCAGTGCCGA CTCCAAGAAT 
TCCAACAGCA ACGTGATCCA AGTGGACCAG TCTGGCCTGG GCTTACCCTC AAGAGATTAT 
TACCTGAACA AAACCGAGAA TGAGAAGGTG CTGACGGGAT ACCTGAACTA CATGGTCCAG 
CTGGGGAAGC TGCTGGGAGG AGGGGCCGAG GACACCATCC GGCCCCAGAT GCAGCAGATC 
CTGGACTTTG AGACGGCGCT GGCCAACATC ACCATCCCCC AGGAGAAGCG CCGGGACGAG 
GAACTCATCT ACCACAAAGT GACGGCGGCT GAGTTGCAGA CCTTGGCGCC CGCCATCAAC 
TGGCTGCCCT TCCTCAACAC CATCTTCTAC CCCGTGGAGA TCAATGAATC AGAGCCTATT 
GTCATCTACG ACAAAGAATA CCTGAGCAAG GTCTCCACCC TCATCAACAG CACAGACAAA 
TGCCTGCTGA ACAACTACAT GATCTGGAAC CTGGTACGGA AGACGAGCTC CTTCCTCGAT 
CAGCGCTTCC AGGACGCCGA CGAGAAGTTC ATGGAAGTCA TGTATGGGAC CAAGAAGACG 
TGTCTTCCCC GCTGGAAGTT TTGTGTGAGT GATACAGAGA ACACCTTGGG CTTCGCCCTG 
GGCCCCATGT TCGTCAAAGC GACCTTCGCT GAGGACAGCA AGAACATAGC CAGCGAGATC 
ATCCTGGAGA TCAAGAAGGC GTTTGAAGAG AGCCTGAGCA CCCTGAAGTG GATGGATGAA 
GATACTCGGA AATCGGCCAA GGAAAAGGCG GACGCGATCT ACAACATGAT AGGCTACCCC 
AACTTTATCA TGGACCCCAA GGAGCTGGAC AAAGTGTTCA ATGACTACAC CGCTGTGCCA 
GACCTCTACT TCGAGAACGC CATGCGGTTT TTCAACTTCT CCTGGAGGGT CACTGCCGAC 
CAGCTCCGGA AAGCGCCCAA CAGAGATCAG TGGAGCATGA CCCCGCCCAT GGTGAACGCC 
TACTACTCGC CCACCAAGAA CGAGATCGTG TTTCCGGCCG GAATCCTGCA GGCGCCATTC 
TACACCCGCT CTTCACCCAA TGCCTTAAAC TTCGGCGGCA TCGGCGTCGT CGTGGGCCAC 
GAGCTGACTC ATGCTTTTGA TGATCAAGGC CGAGAGTACG ACAAGGATGG GAACCTCCGG 
CCCTGGTGGA AGAACTCGTC CGTGGAGGCG TTCAAGCAGC AGACCGCGTG CATGGTGGAG 
CAGTACGGCA ACTATAGCGT GAACGGGGAG CCGGTGAACG GCCGGCACAC CCTCGGCGAA 
AACATCGCCG ACAACGGGGG CCTCAAGGCG GCCTATCGGG CCTACCAGAA CTGGGTCAAG 
AAGAATGGGG CTGAGCAGAC ACTGCCCACC CTGGGTCTCA CCAACAACCA GCTCTTCTTC 
CTGAGTTTTG CACAGGTCTG GTGTTCCGTC CGCACCCCCG AGAGTTCGCA CGAAGGTCTC 
ATCACCGATC CCCACAGCCC CTCCCGCTTC CGGGTCATCG GCTCCATCTC CAACTCCAAG 
GAGTTCTCGG AACACTTCCA CTGCCCGCCC GGCTCACCCA TGAACCCGCA TCACAAGTGT 
GAAGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800825.1
CDS	182..2473
Translation	MVSPWHSPLTSPGTQCSDPWVGSGVHTFGPRSKLDPSSRVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_010802523.2
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802523.2

ORF Insert Sequence:

ATGGTTTCCC CTTGGCATTC CCCACTGACA TCTCCCGGGA CTCAATGCTC TGACCCGTGG 
GTGGGCTCTG GGGTCCATAC CTTTGGGCCC AGATCCAAGC TCGACCCATC TAGCCGTGTG 
AACTTCCGAG GCCCCCGGAA CGGCCAGAGA TGCTGGGCCG CCAGGACCCC GGTGGAGAAG 
CGGCTGGTGG TGCTGGTGGC GCTCCTGGCG GCGGCATTGG TGGCCTGTTT GGCAGTACTG 
GGCATCCAAT ACCAGACAAG AACGCCCTCG GTGTGCCTAA GTGAGGCCTG CATCTCGGTG 
ACCAGCTCCA TCTTGAGTTC CATGGACCCC ACGGTGGACC CCTGCCAGGA CTTCTTCACC 
TATGCCTGTG GCGGCTGGAT CAAAGCCAAC CCCGTGCCGG ATGGCCACTC GCGCTGGGGG 
ACCTTCAGCA ACCTCTGGGA ACACAACCAA GCCATCATCA AGCACCTCCT TGAAAACTCC 
ACGGCCAGCG TGAGCGAGGC AGAGAGGAAG GCCCAGGTGT ACTACCGAGC CTGCATGAAC 
GAAACCAGGA TTGAGGAGCT CAAGGCCAAA CCCCTGATGG AGCTCATTGA GAAGCTCGGC 
GGCTGGAACA TCACGGGGCC CTGGGACAAG GACAACTTCC AGGACACCCT GCAGGTGGTC 
ACATCCCACT ACCACACCTC CCCCTTCTTC TCCGTCTACG TCAGTGCCGA CTCCAAGAAT 
TCCAACAGCA ACGTGATCCA AGTGGACCAG TCTGGCCTGG GCTTACCCTC AAGAGATTAT 
TACCTGAACA AAACCGAGAA TGAGAAGGTG CTGACGGGAT ACCTGAACTA CATGGTCCAG 
CTGGGGAAGC TGCTGGGAGG AGGGGCCGAG GACACCATCC GGCCCCAGAT GCAGCAGATC 
CTGGACTTTG AGACGGCGCT GGCCAACATC ACCATCCCCC AGGAGAAGCG CCGGGACGAG 
GAACTCATCT ACCACAAAGT GACGGCGGCT GAGTTGCAGA CCTTGGCGCC CGCCATCAAC 
TGGCTGCCCT TCCTCAACAC CATCTTCTAC CCCGTGGAGA TCAATGAATC AGAGCCTATT 
GTCATCTACG ACAAAGAATA CCTGAGCAAG GTCTCCACCC TCATCAACAG CACAGACAAA 
TGCCTGCTGA ACAACTACAT GATCTGGAAC CTGGTACGGA AGACGAGCTC CTTCCTCGAT 
CAGCGCTTCC AGGACGCCGA CGAGAAGTTC ATGGAAGTCA TGTATGGGAC CAAGAAGACG 
TGTCTTCCCC GCTGGAAGTT TTGTGTGAGT GATACAGAGA ACACCTTGGG CTTCGCCCTG 
GGCCCCATGT TCGTCAAAGC GACCTTCGCT GAGGACAGCA AGAACATAGC CAGCGAGATC 
ATCCTGGAGA TCAAGAAGGC GTTTGAAGAG AGCCTGAGCA CCCTGAAGTG GATGGATGAA 
GATACTCGGA AATCGGCCAA GGAAAAGGCG GACGCGATCT ACAACATGAT AGGCTACCCC 
AACTTTATCA TGGACCCCAA GGAGCTGGAC AAAGTGTTCA ATGACTACAC CGCTGTGCCA 
GACCTCTACT TCGAGAACGC CATGCGGTTT TTCAACTTCT CCTGGAGGGT CACTGCCGAC 
CAGCTCCGGA AAGCGCCCAA CAGAGATCAG TGGAGCATGA CCCCGCCCAT GGTGAACGCC 
TACTACTCGC CCACCAAGAA CGAGATCGTG TTTCCGGCCG GAATCCTGCA GGCGCCATTC 
TACACCCGCT CTTCACCCAA TGCCTTAAAC TTCGGCGGCA TCGGCGTCGT CGTGGGCCAC 
GAGCTGACTC ATGCTTTTGA TGATCAAGGC CGAGAGTACG ACAAGGATGG GAACCTCCGG 
CCCTGGTGGA AGAACTCGTC CGTGGAGGCG TTCAAGCAGC AGACCGCGTG CATGGTGGAG 
CAGTACGGCA ACTATAGCGT GAACGGGGAG CCGGTGAACG GCCGGCACAC CCTCGGCGAA 
AACATCGCCG ACAACGGGGG CCTCAAGGCG GCCTATCGGG CCTACCAGAA CTGGGTCAAG 
AAGAATGGGG CTGAGCAGAC ACTGCCCACC CTGGGTCTCA CCAACAACCA GCTCTTCTTC 
CTGAGTTTTG CACAGGTCTG GTGTTCCGTC CGCACCCCCG AGAGTTCGCA CGAAGGTCTC 
ATCACCGATC CCCACAGCCC CTCCCGCTTC CGGGTCATCG GCTCCATCTC CAACTCCAAG 
GAGTTCTCGG AACACTTCCA CTGCCCGCCC GGCTCACCCA TGAACCCGCA TCACAAGTGT 
GAAGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_015460016.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315502.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88045
Clone ID Related Accession (Same CDS sequence)	XM_015460016.1
Accession Version	XM_015460016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATC CTTTGGGCAA CTGTTGGGTA GGCTTTTCTG GGACCTTGAG GGACAGGGAG 
GGAGCCGGGT GTCCAGAGCG CCCCACCTCT GAGGGAAGGT GGAGGGAACG GTCCTGTTCC 
TGGCTGTCAG CTCGCCCCAA AGCCGTTCCT GTTTTGAGGA TGTCTACCTA CAAGCGGGCC 
ACGCTGGACG AGGAGGACCT GGTGGACTCG CTGTCCGAGA GCGACGTGTA CCCCAACCAC 
CTGCAGGTGA ACTTCCGAGG CCCCCGGAAC GGCCAGAGAT GCTGGGCCGC CAGGACCCCG 
GTGGAGAAGC GGCTGGTGGT GCTGGTGGCG CTCCTGGCGG CGGCATTGGT GGCCTGTTTG 
GCAGTACTGG GCATCCAATA CCAGACAAGA ACGCCCTCGG TGTGCCTAAG TGAGGCCTGC 
ATCTCGGTGA CCAGCTCCAT CTTGAGTTCC ATGGACCCCA CGGTGGACCC CTGCCAGGAC 
TTCTTCACCT ATGCCTGTGG CGGCTGGATC AAAGCCAACC CCGTGCCGGA TGGCCACTCG 
CGCTGGGGGA CCTTCAGCAA CCTCTGGGAA CACAACCAAG CCATCATCAA GCACCTCCTT 
GAAAACTCCA CGGCCAGCGT GAGCGAGGCA GAGAGGAAGG CCCAGGTGTA CTACCGAGCC 
TGCATGAACG AAACCAGGAT TGAGGAGCTC AAGGCCAAAC CCCTGATGGA GCTCATTGAG 
AAGCTCGGCG GCTGGAACAT CACGGGGCCC TGGGACAAGG ACAACTTCCA GGACACCCTG 
CAGGTGGTCA CATCCCACTA CCACACCTCC CCCTTCTTCT CCGTCTACGT CAGTGCCGAC 
TCCAAGAATT CCAACAGCAA CGTGATCCAA GTGGACCAGT CTGGCCTGGG CTTACCCTCA 
AGAGATTATT ACCTGAACAA AACCGAGAAT GAGAAGGTGC TGACGGGATA CCTGAACTAC 
ATGGTCCAGC TGGGGAAGCT GCTGGGAGGA GGGGCCGAGG ACACCATCCG GCCCCAGATG 
CAGCAGATCC TGGACTTTGA GACGGCGCTG GCCAACATCA CCATCCCCCA GGAGAAGCGC 
CGGGACGAGG AACTCATCTA CCACAAAGTG ACGGCGGCTG AGTTGCAGAC CTTGGCGCCC 
GCCATCAACT GGCTGCCCTT CCTCAACACC ATCTTCTACC CCGTGGAGAT CAATGAATCA 
GAGCCTATTG TCATCTACGA CAAAGAATAC CTGAGCAAGG TCTCCACCCT CATCAACAGC 
ACAGACAAAT GCCTGCTGAA CAACTACATG ATCTGGAACC TGGTACGGAA GACGAGCTCC 
TTCCTCGATC AGCGCTTCCA GGACGCCGAC GAGAAGTTCA TGGAAGTCAT GTATGGGACC 
AAGAAGACGT GTCTTCCCCG CTGGAAGTTT TGTGTGAGTG ATACAGAGAA CACCTTGGGC 
TTCGCCCTGG GCCCCATGTT CGTCAAAGCG ACCTTCGCTG AGGACAGCAA GAACATAGCC 
AGCGAGATCA TCCTGGAGAT CAAGAAGGCG TTTGAAGAGA GCCTGAGCAC CCTGAAGTGG 
ATGGATGAAG ATACTCGGAA ATCGGCCAAG GAAAAGGCGG ACGCGATCTA CAACATGATA 
GGCTACCCCA ACTTTATCAT GGACCCCAAG GAGCTGGACA AAGTGTTCAA TGACTACACC 
GCTGTGCCAG ACCTCTACTT CGAGAACGCC ATGCGGTTTT TCAACTTCTC CTGGAGGGTC 
ACTGCCGACC AGCTCCGGAA AGCGCCCAAC AGAGATCAGT GGAGCATGAC CCCGCCCATG 
GTGAACGCCT ACTACTCGCC CACCAAGAAC GAGATCGTGT TTCCGGCCGG AATCCTGCAG 
GCGCCATTCT ACACCCGCTC TTCACCCAAT GCCTTAAACT TCGGCGGCAT CGGCGTCGTC 
GTGGGCCACG AGCTGACTCA TGCTTTTGAT GATCAAGGCC GAGAGTACGA CAAGGATGGG 
AACCTCCGGC CCTGGTGGAA GAACTCGTCC GTGGAGGCGT TCAAGCAGCA GACCGCGTGC 
ATGGTGGAGC AGTACGGCAA CTATAGCGTG AACGGGGAGC CGGTGAACGG CCGGCACACC 
CTCGGCGAAA ACATCGCCGA CAACGGGGGC CTCAAGGCGG CCTATCGGGC CTACCAGAAC 
TGGGTCAAGA AGAATGGGGC TGAGCAGACA CTGCCCACCC TGGGTCTCAC CAACAACCAG 
CTCTTCTTCC TGAGTTTTGC ACAGGTCTGG TGTTCCGTCC GCACCCCCGA GAGTTCGCAC 
GAAGGTCTCA TCACCGATCC CCACAGCCCC TCCCGCTTCC GGGTCATCGG CTCCATCTCC 
AACTCCAAGG AGTTCTCGGA ACACTTCCAC TGCCCGCCCG GCTCACCCAT GAACCCGCAT 
CACAAGTGTG AAGTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315502.1
CDS	16..2436
Translation	MEDPLGNCWVGFSGTLRDREGAGCPERPTSEGRWRERSCSWLSARPKAVPVLRMSTYKRATLDEEDLVDSLSESDVYPNHLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_015460016.1
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460016.1

ORF Insert Sequence:

ATGGAGGATC CTTTGGGCAA CTGTTGGGTA GGCTTTTCTG GGACCTTGAG GGACAGGGAG 
GGAGCCGGGT GTCCAGAGCG CCCCACCTCT GAGGGAAGGT GGAGGGAACG GTCCTGTTCC 
TGGCTGTCAG CTCGCCCCAA AGCCGTTCCT GTTTTGAGGA TGTCTACCTA CAAGCGGGCC 
ACGCTGGACG AGGAGGACCT GGTGGACTCG CTGTCCGAGA GCGACGTGTA CCCCAACCAC 
CTGCAGGTGA ACTTCCGAGG CCCCCGGAAC GGCCAGAGAT GCTGGGCCGC CAGGACCCCG 
GTGGAGAAGC GGCTGGTGGT GCTGGTGGCG CTCCTGGCGG CGGCATTGGT GGCCTGTTTG 
GCAGTACTGG GCATCCAATA CCAGACAAGA ACGCCCTCGG TGTGCCTAAG TGAGGCCTGC 
ATCTCGGTGA CCAGCTCCAT CTTGAGTTCC ATGGACCCCA CGGTGGACCC CTGCCAGGAC 
TTCTTCACCT ATGCCTGTGG CGGCTGGATC AAAGCCAACC CCGTGCCGGA TGGCCACTCG 
CGCTGGGGGA CCTTCAGCAA CCTCTGGGAA CACAACCAAG CCATCATCAA GCACCTCCTT 
GAAAACTCCA CGGCCAGCGT GAGCGAGGCA GAGAGGAAGG CCCAGGTGTA CTACCGAGCC 
TGCATGAACG AAACCAGGAT TGAGGAGCTC AAGGCCAAAC CCCTGATGGA GCTCATTGAG 
AAGCTCGGCG GCTGGAACAT CACGGGGCCC TGGGACAAGG ACAACTTCCA GGACACCCTG 
CAGGTGGTCA CATCCCACTA CCACACCTCC CCCTTCTTCT CCGTCTACGT CAGTGCCGAC 
TCCAAGAATT CCAACAGCAA CGTGATCCAA GTGGACCAGT CTGGCCTGGG CTTACCCTCA 
AGAGATTATT ACCTGAACAA AACCGAGAAT GAGAAGGTGC TGACGGGATA CCTGAACTAC 
ATGGTCCAGC TGGGGAAGCT GCTGGGAGGA GGGGCCGAGG ACACCATCCG GCCCCAGATG 
CAGCAGATCC TGGACTTTGA GACGGCGCTG GCCAACATCA CCATCCCCCA GGAGAAGCGC 
CGGGACGAGG AACTCATCTA CCACAAAGTG ACGGCGGCTG AGTTGCAGAC CTTGGCGCCC 
GCCATCAACT GGCTGCCCTT CCTCAACACC ATCTTCTACC CCGTGGAGAT CAATGAATCA 
GAGCCTATTG TCATCTACGA CAAAGAATAC CTGAGCAAGG TCTCCACCCT CATCAACAGC 
ACAGACAAAT GCCTGCTGAA CAACTACATG ATCTGGAACC TGGTACGGAA GACGAGCTCC 
TTCCTCGATC AGCGCTTCCA GGACGCCGAC GAGAAGTTCA TGGAAGTCAT GTATGGGACC 
AAGAAGACGT GTCTTCCCCG CTGGAAGTTT TGTGTGAGTG ATACAGAGAA CACCTTGGGC 
TTCGCCCTGG GCCCCATGTT CGTCAAAGCG ACCTTCGCTG AGGACAGCAA GAACATAGCC 
AGCGAGATCA TCCTGGAGAT CAAGAAGGCG TTTGAAGAGA GCCTGAGCAC CCTGAAGTGG 
ATGGATGAAG ATACTCGGAA ATCGGCCAAG GAAAAGGCGG ACGCGATCTA CAACATGATA 
GGCTACCCCA ACTTTATCAT GGACCCCAAG GAGCTGGACA AAGTGTTCAA TGACTACACC 
GCTGTGCCAG ACCTCTACTT CGAGAACGCC ATGCGGTTTT TCAACTTCTC CTGGAGGGTC 
ACTGCCGACC AGCTCCGGAA AGCGCCCAAC AGAGATCAGT GGAGCATGAC CCCGCCCATG 
GTGAACGCCT ACTACTCGCC CACCAAGAAC GAGATCGTGT TTCCGGCCGG AATCCTGCAG 
GCGCCATTCT ACACCCGCTC TTCACCCAAT GCCTTAAACT TCGGCGGCAT CGGCGTCGTC 
GTGGGCCACG AGCTGACTCA TGCTTTTGAT GATCAAGGCC GAGAGTACGA CAAGGATGGG 
AACCTCCGGC CCTGGTGGAA GAACTCGTCC GTGGAGGCGT TCAAGCAGCA GACCGCGTGC 
ATGGTGGAGC AGTACGGCAA CTATAGCGTG AACGGGGAGC CGGTGAACGG CCGGCACACC 
CTCGGCGAAA ACATCGCCGA CAACGGGGGC CTCAAGGCGG CCTATCGGGC CTACCAGAAC 
TGGGTCAAGA AGAATGGGGC TGAGCAGACA CTGCCCACCC TGGGTCTCAC CAACAACCAG 
CTCTTCTTCC TGAGTTTTGC ACAGGTCTGG TGTTCCGTCC GCACCCCCGA GAGTTCGCAC 
GAAGGTCTCA TCACCGATCC CCACAGCCCC TCCCGCTTCC GGGTCATCGG CTCCATCTCC 
AACTCCAAGG AGTTCTCGGA ACACTTCCAC TGCCCGCCCG GCTCACCCAT GAACCCGCAT 
CACAAGTGTG AAGTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_015460021.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315507.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa88046
Clone ID Related Accession (Same CDS sequence)	XM_015460021.2 , XM_015460021.1
Accession Version	XM_015460021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTGTCCGAG 
AGCGACGTGT ACCCCAACCA CCTGCAGGTG AACTTCCGAG GCCCCCGGAA CGGCCAGAGA 
TGCTGGGCCG CCAGGACCCC GGTGGAGAAG CGGCTGGTGG TGCTGGTGGC GCTCCTGGCG 
GCGGCATTGG TGGCCTGTTT GGCAGTACTG GGCATCCAAT ACCAGACAAG AACGCCCTCG 
GTGTGCCTAA GTGAGGCCTG CATCTCGGTG ACCAGCTCCA TCTTGAGTTC CATGGACCCC 
ACGGTGGACC CCTGCCAGGA CTTCTTCACC TATGCCTGTG GCGGCTGGAT CAAAGCCAAC 
CCCGTGCCGG ATGGCCACTC GCGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCCATCATCA AGCACCTCCT TGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGGAAG 
GCCCAGGTGT ACTACCGAGC CTGCATGAAC GAAACCAGGA TTGAGGAGCT CAAGGCCAAA 
CCCCTGATGG AGCTCATTGA GAAGCTCGGC GGCTGGAACA TCACGGGGCC CTGGGACAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACATCCCACT ACCACACCTC CCCCTTCTTC 
TCCGTCTACG TCAGTGCCGA CTCCAAGAAT TCCAACAGCA ACGTGATCCA AGTGGACCAG 
TCTGGCCTGG GCTTACCCTC AAGAGATTAT TACCTGAACA AAACCGAGAA TGAGAAGGTG 
CTGACGGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGAGG AGGGGCCGAG 
GACACCATCC GGCCCCAGAT GCAGCAGATC CTGGACTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CCGGGACGAG GAACTCATCT ACCACAAAGT GACGGCGGCT 
GAGTTGCAGA CCTTGGCGCC CGCCATCAAC TGGCTGCCCT TCCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC AGAGCCTATT GTCATCTACG ACAAAGAATA CCTGAGCAAG 
GTCTCCACCC TCATCAACAG CACAGACAAA TGCCTGCTGA ACAACTACAT GATCTGGAAC 
CTGGTACGGA AGACGAGCTC CTTCCTCGAT CAGCGCTTCC AGGACGCCGA CGAGAAGTTC 
ATGGAAGTCA TGTATGGGAC CAAGAAGACG TGTCTTCCCC GCTGGAAGTT TTGTGTGAGT 
GATACAGAGA ACACCTTGGG CTTCGCCCTG GGCCCCATGT TCGTCAAAGC GACCTTCGCT 
GAGGACAGCA AGAACATAGC CAGCGAGATC ATCCTGGAGA TCAAGAAGGC GTTTGAAGAG 
AGCCTGAGCA CCCTGAAGTG GATGGATGAA GATACTCGGA AATCGGCCAA GGAAAAGGCG 
GACGCGATCT ACAACATGAT AGGCTACCCC AACTTTATCA TGGACCCCAA GGAGCTGGAC 
AAAGTGTTCA ATGACTACAC CGCTGTGCCA GACCTCTACT TCGAGAACGC CATGCGGTTT 
TTCAACTTCT CCTGGAGGGT CACTGCCGAC CAGCTCCGGA AAGCGCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCGGCCG GAATCCTGCA GGCGCCATTC TACACCCGCT CTTCACCCAA TGCCTTAAAC 
TTCGGCGGCA TCGGCGTCGT CGTGGGCCAC GAGCTGACTC ATGCTTTTGA TGATCAAGGC 
CGAGAGTACG ACAAGGATGG GAACCTCCGG CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACCGCGTG CATGGTGGAG CAGTACGGCA ACTATAGCGT GAACGGGGAG 
CCGGTGAACG GCCGGCACAC CCTCGGCGAA AACATCGCCG ACAACGGGGG CCTCAAGGCG 
GCCTATCGGG CCTACCAGAA CTGGGTCAAG AAGAATGGGG CTGAGCAGAC ACTGCCCACC 
CTGGGTCTCA CCAACAACCA GCTCTTCTTC CTGAGTTTTG CACAGGTCTG GTGTTCCGTC 
CGCACCCCCG AGAGTTCGCA CGAAGGTCTC ATCACCGATC CCCACAGCCC CTCCCGCTTC 
CGGGTCATCG GCTCCATCTC CAACTCCAAG GAGTTCTCGG AACACTTCCA CTGCCCGCCC 
GGCTCACCCA TGAACCCGCA TCACAAGTGT GAAGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315507.1
CDS	132..2393
Translation	MSTYKRATLDEEDLVDSLSESDVYPNHLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_015460021.2
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460021.2

ORF Insert Sequence:

ATGTCTACCT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTGTCCGAG 
AGCGACGTGT ACCCCAACCA CCTGCAGGTG AACTTCCGAG GCCCCCGGAA CGGCCAGAGA 
TGCTGGGCCG CCAGGACCCC GGTGGAGAAG CGGCTGGTGG TGCTGGTGGC GCTCCTGGCG 
GCGGCATTGG TGGCCTGTTT GGCAGTACTG GGCATCCAAT ACCAGACAAG AACGCCCTCG 
GTGTGCCTAA GTGAGGCCTG CATCTCGGTG ACCAGCTCCA TCTTGAGTTC CATGGACCCC 
ACGGTGGACC CCTGCCAGGA CTTCTTCACC TATGCCTGTG GCGGCTGGAT CAAAGCCAAC 
CCCGTGCCGG ATGGCCACTC GCGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCCATCATCA AGCACCTCCT TGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGGAAG 
GCCCAGGTGT ACTACCGAGC CTGCATGAAC GAAACCAGGA TTGAGGAGCT CAAGGCCAAA 
CCCCTGATGG AGCTCATTGA GAAGCTCGGC GGCTGGAACA TCACGGGGCC CTGGGACAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACATCCCACT ACCACACCTC CCCCTTCTTC 
TCCGTCTACG TCAGTGCCGA CTCCAAGAAT TCCAACAGCA ACGTGATCCA AGTGGACCAG 
TCTGGCCTGG GCTTACCCTC AAGAGATTAT TACCTGAACA AAACCGAGAA TGAGAAGGTG 
CTGACGGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGAGG AGGGGCCGAG 
GACACCATCC GGCCCCAGAT GCAGCAGATC CTGGACTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CCGGGACGAG GAACTCATCT ACCACAAAGT GACGGCGGCT 
GAGTTGCAGA CCTTGGCGCC CGCCATCAAC TGGCTGCCCT TCCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC AGAGCCTATT GTCATCTACG ACAAAGAATA CCTGAGCAAG 
GTCTCCACCC TCATCAACAG CACAGACAAA TGCCTGCTGA ACAACTACAT GATCTGGAAC 
CTGGTACGGA AGACGAGCTC CTTCCTCGAT CAGCGCTTCC AGGACGCCGA CGAGAAGTTC 
ATGGAAGTCA TGTATGGGAC CAAGAAGACG TGTCTTCCCC GCTGGAAGTT TTGTGTGAGT 
GATACAGAGA ACACCTTGGG CTTCGCCCTG GGCCCCATGT TCGTCAAAGC GACCTTCGCT 
GAGGACAGCA AGAACATAGC CAGCGAGATC ATCCTGGAGA TCAAGAAGGC GTTTGAAGAG 
AGCCTGAGCA CCCTGAAGTG GATGGATGAA GATACTCGGA AATCGGCCAA GGAAAAGGCG 
GACGCGATCT ACAACATGAT AGGCTACCCC AACTTTATCA TGGACCCCAA GGAGCTGGAC 
AAAGTGTTCA ATGACTACAC CGCTGTGCCA GACCTCTACT TCGAGAACGC CATGCGGTTT 
TTCAACTTCT CCTGGAGGGT CACTGCCGAC CAGCTCCGGA AAGCGCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCGGCCG GAATCCTGCA GGCGCCATTC TACACCCGCT CTTCACCCAA TGCCTTAAAC 
TTCGGCGGCA TCGGCGTCGT CGTGGGCCAC GAGCTGACTC ATGCTTTTGA TGATCAAGGC 
CGAGAGTACG ACAAGGATGG GAACCTCCGG CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACCGCGTG CATGGTGGAG CAGTACGGCA ACTATAGCGT GAACGGGGAG 
CCGGTGAACG GCCGGCACAC CCTCGGCGAA AACATCGCCG ACAACGGGGG CCTCAAGGCG 
GCCTATCGGG CCTACCAGAA CTGGGTCAAG AAGAATGGGG CTGAGCAGAC ACTGCCCACC 
CTGGGTCTCA CCAACAACCA GCTCTTCTTC CTGAGTTTTG CACAGGTCTG GTGTTCCGTC 
CGCACCCCCG AGAGTTCGCA CGAAGGTCTC ATCACCGATC CCCACAGCCC CTCCCGCTTC 
CGGGTCATCG GCTCCATCTC CAACTCCAAG GAGTTCTCGG AACACTTCCA CTGCCCGCCC 
GGCTCACCCA TGAACCCGCA TCACAAGTGT GAAGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_015460021.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315507.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa88046
Clone ID Related Accession (Same CDS sequence)	XM_015460021.2 , XM_015460021.1
Accession Version	XM_015460021.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTGTCCGAG 
AGCGACGTGT ACCCCAACCA CCTGCAGGTG AACTTCCGAG GCCCCCGGAA CGGCCAGAGA 
TGCTGGGCCG CCAGGACCCC GGTGGAGAAG CGGCTGGTGG TGCTGGTGGC GCTCCTGGCG 
GCGGCATTGG TGGCCTGTTT GGCAGTACTG GGCATCCAAT ACCAGACAAG AACGCCCTCG 
GTGTGCCTAA GTGAGGCCTG CATCTCGGTG ACCAGCTCCA TCTTGAGTTC CATGGACCCC 
ACGGTGGACC CCTGCCAGGA CTTCTTCACC TATGCCTGTG GCGGCTGGAT CAAAGCCAAC 
CCCGTGCCGG ATGGCCACTC GCGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCCATCATCA AGCACCTCCT TGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGGAAG 
GCCCAGGTGT ACTACCGAGC CTGCATGAAC GAAACCAGGA TTGAGGAGCT CAAGGCCAAA 
CCCCTGATGG AGCTCATTGA GAAGCTCGGC GGCTGGAACA TCACGGGGCC CTGGGACAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACATCCCACT ACCACACCTC CCCCTTCTTC 
TCCGTCTACG TCAGTGCCGA CTCCAAGAAT TCCAACAGCA ACGTGATCCA AGTGGACCAG 
TCTGGCCTGG GCTTACCCTC AAGAGATTAT TACCTGAACA AAACCGAGAA TGAGAAGGTG 
CTGACGGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGAGG AGGGGCCGAG 
GACACCATCC GGCCCCAGAT GCAGCAGATC CTGGACTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CCGGGACGAG GAACTCATCT ACCACAAAGT GACGGCGGCT 
GAGTTGCAGA CCTTGGCGCC CGCCATCAAC TGGCTGCCCT TCCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC AGAGCCTATT GTCATCTACG ACAAAGAATA CCTGAGCAAG 
GTCTCCACCC TCATCAACAG CACAGACAAA TGCCTGCTGA ACAACTACAT GATCTGGAAC 
CTGGTACGGA AGACGAGCTC CTTCCTCGAT CAGCGCTTCC AGGACGCCGA CGAGAAGTTC 
ATGGAAGTCA TGTATGGGAC CAAGAAGACG TGTCTTCCCC GCTGGAAGTT TTGTGTGAGT 
GATACAGAGA ACACCTTGGG CTTCGCCCTG GGCCCCATGT TCGTCAAAGC GACCTTCGCT 
GAGGACAGCA AGAACATAGC CAGCGAGATC ATCCTGGAGA TCAAGAAGGC GTTTGAAGAG 
AGCCTGAGCA CCCTGAAGTG GATGGATGAA GATACTCGGA AATCGGCCAA GGAAAAGGCG 
GACGCGATCT ACAACATGAT AGGCTACCCC AACTTTATCA TGGACCCCAA GGAGCTGGAC 
AAAGTGTTCA ATGACTACAC CGCTGTGCCA GACCTCTACT TCGAGAACGC CATGCGGTTT 
TTCAACTTCT CCTGGAGGGT CACTGCCGAC CAGCTCCGGA AAGCGCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCGGCCG GAATCCTGCA GGCGCCATTC TACACCCGCT CTTCACCCAA TGCCTTAAAC 
TTCGGCGGCA TCGGCGTCGT CGTGGGCCAC GAGCTGACTC ATGCTTTTGA TGATCAAGGC 
CGAGAGTACG ACAAGGATGG GAACCTCCGG CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACCGCGTG CATGGTGGAG CAGTACGGCA ACTATAGCGT GAACGGGGAG 
CCGGTGAACG GCCGGCACAC CCTCGGCGAA AACATCGCCG ACAACGGGGG CCTCAAGGCG 
GCCTATCGGG CCTACCAGAA CTGGGTCAAG AAGAATGGGG CTGAGCAGAC ACTGCCCACC 
CTGGGTCTCA CCAACAACCA GCTCTTCTTC CTGAGTTTTG CACAGGTCTG GTGTTCCGTC 
CGCACCCCCG AGAGTTCGCA CGAAGGTCTC ATCACCGATC CCCACAGCCC CTCCCGCTTC 
CGGGTCATCG GCTCCATCTC CAACTCCAAG GAGTTCTCGG AACACTTCCA CTGCCCGCCC 
GGCTCACCCA TGAACCCGCA TCACAAGTGT GAAGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315507.1
CDS	20..2281
Translation	MSTYKRATLDEEDLVDSLSESDVYPNHLQVNFRGPRNGQRCWAARTPVEKRLVVLVALLAAALVACLAVLGIQYQTRTPSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_015460021.1
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460021.1

ORF Insert Sequence:

ATGTCTACCT ACAAGCGGGC CACGCTGGAC GAGGAGGACC TGGTGGACTC GCTGTCCGAG 
AGCGACGTGT ACCCCAACCA CCTGCAGGTG AACTTCCGAG GCCCCCGGAA CGGCCAGAGA 
TGCTGGGCCG CCAGGACCCC GGTGGAGAAG CGGCTGGTGG TGCTGGTGGC GCTCCTGGCG 
GCGGCATTGG TGGCCTGTTT GGCAGTACTG GGCATCCAAT ACCAGACAAG AACGCCCTCG 
GTGTGCCTAA GTGAGGCCTG CATCTCGGTG ACCAGCTCCA TCTTGAGTTC CATGGACCCC 
ACGGTGGACC CCTGCCAGGA CTTCTTCACC TATGCCTGTG GCGGCTGGAT CAAAGCCAAC 
CCCGTGCCGG ATGGCCACTC GCGCTGGGGG ACCTTCAGCA ACCTCTGGGA ACACAACCAA 
GCCATCATCA AGCACCTCCT TGAAAACTCC ACGGCCAGCG TGAGCGAGGC AGAGAGGAAG 
GCCCAGGTGT ACTACCGAGC CTGCATGAAC GAAACCAGGA TTGAGGAGCT CAAGGCCAAA 
CCCCTGATGG AGCTCATTGA GAAGCTCGGC GGCTGGAACA TCACGGGGCC CTGGGACAAG 
GACAACTTCC AGGACACCCT GCAGGTGGTC ACATCCCACT ACCACACCTC CCCCTTCTTC 
TCCGTCTACG TCAGTGCCGA CTCCAAGAAT TCCAACAGCA ACGTGATCCA AGTGGACCAG 
TCTGGCCTGG GCTTACCCTC AAGAGATTAT TACCTGAACA AAACCGAGAA TGAGAAGGTG 
CTGACGGGAT ACCTGAACTA CATGGTCCAG CTGGGGAAGC TGCTGGGAGG AGGGGCCGAG 
GACACCATCC GGCCCCAGAT GCAGCAGATC CTGGACTTTG AGACGGCGCT GGCCAACATC 
ACCATCCCCC AGGAGAAGCG CCGGGACGAG GAACTCATCT ACCACAAAGT GACGGCGGCT 
GAGTTGCAGA CCTTGGCGCC CGCCATCAAC TGGCTGCCCT TCCTCAACAC CATCTTCTAC 
CCCGTGGAGA TCAATGAATC AGAGCCTATT GTCATCTACG ACAAAGAATA CCTGAGCAAG 
GTCTCCACCC TCATCAACAG CACAGACAAA TGCCTGCTGA ACAACTACAT GATCTGGAAC 
CTGGTACGGA AGACGAGCTC CTTCCTCGAT CAGCGCTTCC AGGACGCCGA CGAGAAGTTC 
ATGGAAGTCA TGTATGGGAC CAAGAAGACG TGTCTTCCCC GCTGGAAGTT TTGTGTGAGT 
GATACAGAGA ACACCTTGGG CTTCGCCCTG GGCCCCATGT TCGTCAAAGC GACCTTCGCT 
GAGGACAGCA AGAACATAGC CAGCGAGATC ATCCTGGAGA TCAAGAAGGC GTTTGAAGAG 
AGCCTGAGCA CCCTGAAGTG GATGGATGAA GATACTCGGA AATCGGCCAA GGAAAAGGCG 
GACGCGATCT ACAACATGAT AGGCTACCCC AACTTTATCA TGGACCCCAA GGAGCTGGAC 
AAAGTGTTCA ATGACTACAC CGCTGTGCCA GACCTCTACT TCGAGAACGC CATGCGGTTT 
TTCAACTTCT CCTGGAGGGT CACTGCCGAC CAGCTCCGGA AAGCGCCCAA CAGAGATCAG 
TGGAGCATGA CCCCGCCCAT GGTGAACGCC TACTACTCGC CCACCAAGAA CGAGATCGTG 
TTTCCGGCCG GAATCCTGCA GGCGCCATTC TACACCCGCT CTTCACCCAA TGCCTTAAAC 
TTCGGCGGCA TCGGCGTCGT CGTGGGCCAC GAGCTGACTC ATGCTTTTGA TGATCAAGGC 
CGAGAGTACG ACAAGGATGG GAACCTCCGG CCCTGGTGGA AGAACTCGTC CGTGGAGGCG 
TTCAAGCAGC AGACCGCGTG CATGGTGGAG CAGTACGGCA ACTATAGCGT GAACGGGGAG 
CCGGTGAACG GCCGGCACAC CCTCGGCGAA AACATCGCCG ACAACGGGGG CCTCAAGGCG 
GCCTATCGGG CCTACCAGAA CTGGGTCAAG AAGAATGGGG CTGAGCAGAC ACTGCCCACC 
CTGGGTCTCA CCAACAACCA GCTCTTCTTC CTGAGTTTTG CACAGGTCTG GTGTTCCGTC 
CGCACCCCCG AGAGTTCGCA CGAAGGTCTC ATCACCGATC CCCACAGCCC CTCCCGCTTC 
CGGGTCATCG GCTCCATCTC CAACTCCAAG GAGTTCTCGG AACACTTCCA CTGCCCGCCC 
GGCTCACCCA TGAACCCGCA TCACAAGTGT GAAGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_015460022.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315508.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X6, mRNA.

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CloneID	OBa88047
Clone ID Related Accession (Same CDS sequence)	XM_015460022.1 , XM_015460022.2
Accession Version	XM_015460022.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCA CGGTGGACCC CTGCCAGGAC TTCTTCACCT ATGCCTGTGG CGGCTGGATC 
AAAGCCAACC CCGTGCCGGA TGGCCACTCG CGCTGGGGGA CCTTCAGCAA CCTCTGGGAA 
CACAACCAAG CCATCATCAA GCACCTCCTT GAAAACTCCA CGGCCAGCGT GAGCGAGGCA 
GAGAGGAAGG CCCAGGTGTA CTACCGAGCC TGCATGAACG AAACCAGGAT TGAGGAGCTC 
AAGGCCAAAC CCCTGATGGA GCTCATTGAG AAGCTCGGCG GCTGGAACAT CACGGGGCCC 
TGGGACAAGG ACAACTTCCA GGACACCCTG CAGGTGGTCA CATCCCACTA CCACACCTCC 
CCCTTCTTCT CCGTCTACGT CAGTGCCGAC TCCAAGAATT CCAACAGCAA CGTGATCCAA 
GTGGACCAGT CTGGCCTGGG CTTACCCTCA AGAGATTATT ACCTGAACAA AACCGAGAAT 
GAGAAGGTGC TGACGGGATA CCTGAACTAC ATGGTCCAGC TGGGGAAGCT GCTGGGAGGA 
GGGGCCGAGG ACACCATCCG GCCCCAGATG CAGCAGATCC TGGACTTTGA GACGGCGCTG 
GCCAACATCA CCATCCCCCA GGAGAAGCGC CGGGACGAGG AACTCATCTA CCACAAAGTG 
ACGGCGGCTG AGTTGCAGAC CTTGGCGCCC GCCATCAACT GGCTGCCCTT CCTCAACACC 
ATCTTCTACC CCGTGGAGAT CAATGAATCA GAGCCTATTG TCATCTACGA CAAAGAATAC 
CTGAGCAAGG TCTCCACCCT CATCAACAGC ACAGACAAAT GCCTGCTGAA CAACTACATG 
ATCTGGAACC TGGTACGGAA GACGAGCTCC TTCCTCGATC AGCGCTTCCA GGACGCCGAC 
GAGAAGTTCA TGGAAGTCAT GTATGGGACC AAGAAGACGT GTCTTCCCCG CTGGAAGTTT 
TGTGTGAGTG ATACAGAGAA CACCTTGGGC TTCGCCCTGG GCCCCATGTT CGTCAAAGCG 
ACCTTCGCTG AGGACAGCAA GAACATAGCC AGCGAGATCA TCCTGGAGAT CAAGAAGGCG 
TTTGAAGAGA GCCTGAGCAC CCTGAAGTGG ATGGATGAAG ATACTCGGAA ATCGGCCAAG 
GAAAAGGCGG ACGCGATCTA CAACATGATA GGCTACCCCA ACTTTATCAT GGACCCCAAG 
GAGCTGGACA AAGTGTTCAA TGACTACACC GCTGTGCCAG ACCTCTACTT CGAGAACGCC 
ATGCGGTTTT TCAACTTCTC CTGGAGGGTC ACTGCCGACC AGCTCCGGAA AGCGCCCAAC 
AGAGATCAGT GGAGCATGAC CCCGCCCATG GTGAACGCCT ACTACTCGCC CACCAAGAAC 
GAGATCGTGT TTCCGGCCGG AATCCTGCAG GCGCCATTCT ACACCCGCTC TTCACCCAAT 
GCCTTAAACT TCGGCGGCAT CGGCGTCGTC GTGGGCCACG AGCTGACTCA TGCTTTTGAT 
GATCAAGGCC GAGAGTACGA CAAGGATGGG AACCTCCGGC CCTGGTGGAA GAACTCGTCC 
GTGGAGGCGT TCAAGCAGCA GACCGCGTGC ATGGTGGAGC AGTACGGCAA CTATAGCGTG 
AACGGGGAGC CGGTGAACGG CCGGCACACC CTCGGCGAAA ACATCGCCGA CAACGGGGGC 
CTCAAGGCGG CCTATCGGGC CTACCAGAAC TGGGTCAAGA AGAATGGGGC TGAGCAGACA 
CTGCCCACCC TGGGTCTCAC CAACAACCAG CTCTTCTTCC TGAGTTTTGC ACAGGTCTGG 
TGTTCCGTCC GCACCCCCGA GAGTTCGCAC GAAGGTCTCA TCACCGATCC CCACAGCCCC 
TCCCGCTTCC GGGTCATCGG CTCCATCTCC AACTCCAAGG AGTTCTCGGA ACACTTCCAC 
TGCCCGCCCG GCTCACCCAT GAACCCGCAT CACAAGTGTG AAGTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315508.1
CDS	337..2307
Translation	MDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_015460022.1
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460022.1

ORF Insert Sequence:

ATGGACCCCA CGGTGGACCC CTGCCAGGAC TTCTTCACCT ATGCCTGTGG CGGCTGGATC 
AAAGCCAACC CCGTGCCGGA TGGCCACTCG CGCTGGGGGA CCTTCAGCAA CCTCTGGGAA 
CACAACCAAG CCATCATCAA GCACCTCCTT GAAAACTCCA CGGCCAGCGT GAGCGAGGCA 
GAGAGGAAGG CCCAGGTGTA CTACCGAGCC TGCATGAACG AAACCAGGAT TGAGGAGCTC 
AAGGCCAAAC CCCTGATGGA GCTCATTGAG AAGCTCGGCG GCTGGAACAT CACGGGGCCC 
TGGGACAAGG ACAACTTCCA GGACACCCTG CAGGTGGTCA CATCCCACTA CCACACCTCC 
CCCTTCTTCT CCGTCTACGT CAGTGCCGAC TCCAAGAATT CCAACAGCAA CGTGATCCAA 
GTGGACCAGT CTGGCCTGGG CTTACCCTCA AGAGATTATT ACCTGAACAA AACCGAGAAT 
GAGAAGGTGC TGACGGGATA CCTGAACTAC ATGGTCCAGC TGGGGAAGCT GCTGGGAGGA 
GGGGCCGAGG ACACCATCCG GCCCCAGATG CAGCAGATCC TGGACTTTGA GACGGCGCTG 
GCCAACATCA CCATCCCCCA GGAGAAGCGC CGGGACGAGG AACTCATCTA CCACAAAGTG 
ACGGCGGCTG AGTTGCAGAC CTTGGCGCCC GCCATCAACT GGCTGCCCTT CCTCAACACC 
ATCTTCTACC CCGTGGAGAT CAATGAATCA GAGCCTATTG TCATCTACGA CAAAGAATAC 
CTGAGCAAGG TCTCCACCCT CATCAACAGC ACAGACAAAT GCCTGCTGAA CAACTACATG 
ATCTGGAACC TGGTACGGAA GACGAGCTCC TTCCTCGATC AGCGCTTCCA GGACGCCGAC 
GAGAAGTTCA TGGAAGTCAT GTATGGGACC AAGAAGACGT GTCTTCCCCG CTGGAAGTTT 
TGTGTGAGTG ATACAGAGAA CACCTTGGGC TTCGCCCTGG GCCCCATGTT CGTCAAAGCG 
ACCTTCGCTG AGGACAGCAA GAACATAGCC AGCGAGATCA TCCTGGAGAT CAAGAAGGCG 
TTTGAAGAGA GCCTGAGCAC CCTGAAGTGG ATGGATGAAG ATACTCGGAA ATCGGCCAAG 
GAAAAGGCGG ACGCGATCTA CAACATGATA GGCTACCCCA ACTTTATCAT GGACCCCAAG 
GAGCTGGACA AAGTGTTCAA TGACTACACC GCTGTGCCAG ACCTCTACTT CGAGAACGCC 
ATGCGGTTTT TCAACTTCTC CTGGAGGGTC ACTGCCGACC AGCTCCGGAA AGCGCCCAAC 
AGAGATCAGT GGAGCATGAC CCCGCCCATG GTGAACGCCT ACTACTCGCC CACCAAGAAC 
GAGATCGTGT TTCCGGCCGG AATCCTGCAG GCGCCATTCT ACACCCGCTC TTCACCCAAT 
GCCTTAAACT TCGGCGGCAT CGGCGTCGTC GTGGGCCACG AGCTGACTCA TGCTTTTGAT 
GATCAAGGCC GAGAGTACGA CAAGGATGGG AACCTCCGGC CCTGGTGGAA GAACTCGTCC 
GTGGAGGCGT TCAAGCAGCA GACCGCGTGC ATGGTGGAGC AGTACGGCAA CTATAGCGTG 
AACGGGGAGC CGGTGAACGG CCGGCACACC CTCGGCGAAA ACATCGCCGA CAACGGGGGC 
CTCAAGGCGG CCTATCGGGC CTACCAGAAC TGGGTCAAGA AGAATGGGGC TGAGCAGACA 
CTGCCCACCC TGGGTCTCAC CAACAACCAG CTCTTCTTCC TGAGTTTTGC ACAGGTCTGG 
TGTTCCGTCC GCACCCCCGA GAGTTCGCAC GAAGGTCTCA TCACCGATCC CCACAGCCCC 
TCCCGCTTCC GGGTCATCGG CTCCATCTCC AACTCCAAGG AGTTCTCGGA ACACTTCCAC 
TGCCCGCCCG GCTCACCCAT GAACCCGCAT CACAAGTGTG AAGTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE1 ( XM_015460022.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315508.1 Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa88047
Clone ID Related Accession (Same CDS sequence)	XM_015460022.1 , XM_015460022.2
Accession Version	XM_015460022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endothelin-converting enzyme 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCA CGGTGGACCC CTGCCAGGAC TTCTTCACCT ATGCCTGTGG CGGCTGGATC 
AAAGCCAACC CCGTGCCGGA TGGCCACTCG CGCTGGGGGA CCTTCAGCAA CCTCTGGGAA 
CACAACCAAG CCATCATCAA GCACCTCCTT GAAAACTCCA CGGCCAGCGT GAGCGAGGCA 
GAGAGGAAGG CCCAGGTGTA CTACCGAGCC TGCATGAACG AAACCAGGAT TGAGGAGCTC 
AAGGCCAAAC CCCTGATGGA GCTCATTGAG AAGCTCGGCG GCTGGAACAT CACGGGGCCC 
TGGGACAAGG ACAACTTCCA GGACACCCTG CAGGTGGTCA CATCCCACTA CCACACCTCC 
CCCTTCTTCT CCGTCTACGT CAGTGCCGAC TCCAAGAATT CCAACAGCAA CGTGATCCAA 
GTGGACCAGT CTGGCCTGGG CTTACCCTCA AGAGATTATT ACCTGAACAA AACCGAGAAT 
GAGAAGGTGC TGACGGGATA CCTGAACTAC ATGGTCCAGC TGGGGAAGCT GCTGGGAGGA 
GGGGCCGAGG ACACCATCCG GCCCCAGATG CAGCAGATCC TGGACTTTGA GACGGCGCTG 
GCCAACATCA CCATCCCCCA GGAGAAGCGC CGGGACGAGG AACTCATCTA CCACAAAGTG 
ACGGCGGCTG AGTTGCAGAC CTTGGCGCCC GCCATCAACT GGCTGCCCTT CCTCAACACC 
ATCTTCTACC CCGTGGAGAT CAATGAATCA GAGCCTATTG TCATCTACGA CAAAGAATAC 
CTGAGCAAGG TCTCCACCCT CATCAACAGC ACAGACAAAT GCCTGCTGAA CAACTACATG 
ATCTGGAACC TGGTACGGAA GACGAGCTCC TTCCTCGATC AGCGCTTCCA GGACGCCGAC 
GAGAAGTTCA TGGAAGTCAT GTATGGGACC AAGAAGACGT GTCTTCCCCG CTGGAAGTTT 
TGTGTGAGTG ATACAGAGAA CACCTTGGGC TTCGCCCTGG GCCCCATGTT CGTCAAAGCG 
ACCTTCGCTG AGGACAGCAA GAACATAGCC AGCGAGATCA TCCTGGAGAT CAAGAAGGCG 
TTTGAAGAGA GCCTGAGCAC CCTGAAGTGG ATGGATGAAG ATACTCGGAA ATCGGCCAAG 
GAAAAGGCGG ACGCGATCTA CAACATGATA GGCTACCCCA ACTTTATCAT GGACCCCAAG 
GAGCTGGACA AAGTGTTCAA TGACTACACC GCTGTGCCAG ACCTCTACTT CGAGAACGCC 
ATGCGGTTTT TCAACTTCTC CTGGAGGGTC ACTGCCGACC AGCTCCGGAA AGCGCCCAAC 
AGAGATCAGT GGAGCATGAC CCCGCCCATG GTGAACGCCT ACTACTCGCC CACCAAGAAC 
GAGATCGTGT TTCCGGCCGG AATCCTGCAG GCGCCATTCT ACACCCGCTC TTCACCCAAT 
GCCTTAAACT TCGGCGGCAT CGGCGTCGTC GTGGGCCACG AGCTGACTCA TGCTTTTGAT 
GATCAAGGCC GAGAGTACGA CAAGGATGGG AACCTCCGGC CCTGGTGGAA GAACTCGTCC 
GTGGAGGCGT TCAAGCAGCA GACCGCGTGC ATGGTGGAGC AGTACGGCAA CTATAGCGTG 
AACGGGGAGC CGGTGAACGG CCGGCACACC CTCGGCGAAA ACATCGCCGA CAACGGGGGC 
CTCAAGGCGG CCTATCGGGC CTACCAGAAC TGGGTCAAGA AGAATGGGGC TGAGCAGACA 
CTGCCCACCC TGGGTCTCAC CAACAACCAG CTCTTCTTCC TGAGTTTTGC ACAGGTCTGG 
TGTTCCGTCC GCACCCCCGA GAGTTCGCAC GAAGGTCTCA TCACCGATCC CCACAGCCCC 
TCCCGCTTCC GGGTCATCGG CTCCATCTCC AACTCCAAGG AGTTCTCGGA ACACTTCCAC 
TGCCCGCCCG GCTCACCCAT GAACCCGCAT CACAAGTGTG AAGTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315508.1
CDS	342..2312
Translation	MDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSHYHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEVW

Target ORF information:

RefSeq Version	XM_015460022.2
Organism	Bos taurus(cattle)
Definition	Bos taurus endothelin converting enzyme 1 (ECE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460022.2

ORF Insert Sequence:

ATGGACCCCA CGGTGGACCC CTGCCAGGAC TTCTTCACCT ATGCCTGTGG CGGCTGGATC 
AAAGCCAACC CCGTGCCGGA TGGCCACTCG CGCTGGGGGA CCTTCAGCAA CCTCTGGGAA 
CACAACCAAG CCATCATCAA GCACCTCCTT GAAAACTCCA CGGCCAGCGT GAGCGAGGCA 
GAGAGGAAGG CCCAGGTGTA CTACCGAGCC TGCATGAACG AAACCAGGAT TGAGGAGCTC 
AAGGCCAAAC CCCTGATGGA GCTCATTGAG AAGCTCGGCG GCTGGAACAT CACGGGGCCC 
TGGGACAAGG ACAACTTCCA GGACACCCTG CAGGTGGTCA CATCCCACTA CCACACCTCC 
CCCTTCTTCT CCGTCTACGT CAGTGCCGAC TCCAAGAATT CCAACAGCAA CGTGATCCAA 
GTGGACCAGT CTGGCCTGGG CTTACCCTCA AGAGATTATT ACCTGAACAA AACCGAGAAT 
GAGAAGGTGC TGACGGGATA CCTGAACTAC ATGGTCCAGC TGGGGAAGCT GCTGGGAGGA 
GGGGCCGAGG ACACCATCCG GCCCCAGATG CAGCAGATCC TGGACTTTGA GACGGCGCTG 
GCCAACATCA CCATCCCCCA GGAGAAGCGC CGGGACGAGG AACTCATCTA CCACAAAGTG 
ACGGCGGCTG AGTTGCAGAC CTTGGCGCCC GCCATCAACT GGCTGCCCTT CCTCAACACC 
ATCTTCTACC CCGTGGAGAT CAATGAATCA GAGCCTATTG TCATCTACGA CAAAGAATAC 
CTGAGCAAGG TCTCCACCCT CATCAACAGC ACAGACAAAT GCCTGCTGAA CAACTACATG 
ATCTGGAACC TGGTACGGAA GACGAGCTCC TTCCTCGATC AGCGCTTCCA GGACGCCGAC 
GAGAAGTTCA TGGAAGTCAT GTATGGGACC AAGAAGACGT GTCTTCCCCG CTGGAAGTTT 
TGTGTGAGTG ATACAGAGAA CACCTTGGGC TTCGCCCTGG GCCCCATGTT CGTCAAAGCG 
ACCTTCGCTG AGGACAGCAA GAACATAGCC AGCGAGATCA TCCTGGAGAT CAAGAAGGCG 
TTTGAAGAGA GCCTGAGCAC CCTGAAGTGG ATGGATGAAG ATACTCGGAA ATCGGCCAAG 
GAAAAGGCGG ACGCGATCTA CAACATGATA GGCTACCCCA ACTTTATCAT GGACCCCAAG 
GAGCTGGACA AAGTGTTCAA TGACTACACC GCTGTGCCAG ACCTCTACTT CGAGAACGCC 
ATGCGGTTTT TCAACTTCTC CTGGAGGGTC ACTGCCGACC AGCTCCGGAA AGCGCCCAAC 
AGAGATCAGT GGAGCATGAC CCCGCCCATG GTGAACGCCT ACTACTCGCC CACCAAGAAC 
GAGATCGTGT TTCCGGCCGG AATCCTGCAG GCGCCATTCT ACACCCGCTC TTCACCCAAT 
GCCTTAAACT TCGGCGGCAT CGGCGTCGTC GTGGGCCACG AGCTGACTCA TGCTTTTGAT 
GATCAAGGCC GAGAGTACGA CAAGGATGGG AACCTCCGGC CCTGGTGGAA GAACTCGTCC 
GTGGAGGCGT TCAAGCAGCA GACCGCGTGC ATGGTGGAGC AGTACGGCAA CTATAGCGTG 
AACGGGGAGC CGGTGAACGG CCGGCACACC CTCGGCGAAA ACATCGCCGA CAACGGGGGC 
CTCAAGGCGG CCTATCGGGC CTACCAGAAC TGGGTCAAGA AGAATGGGGC TGAGCAGACA 
CTGCCCACCC TGGGTCTCAC CAACAACCAG CTCTTCTTCC TGAGTTTTGC ACAGGTCTGG 
TGTTCCGTCC GCACCCCCGA GAGTTCGCAC GAAGGTCTCA TCACCGATCC CCACAGCCCC 
TCCCGCTTCC GGGTCATCGG CTCCATCTCC AACTCCAAGG AGTTCTCGGA ACACTTCCAC 
TGCCCGCCCG GCTCACCCAT GAACCCGCAT CACAAGTGTG AAGTCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ECE1
Entrez Gene ID	281133
Full Name	endothelin converting enzyme 1
General protein information	Preferred Names endothelin converting enzyme 1 Names endothelin-converting enzyme 1 ECE-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	ECE1	XP_001162780.1
Mus musculus (house mouse)	Ece1	NP_955011.1
Bos taurus (cattle)	ECE1	NP_851352.1
Rattus norvegicus (Norway rat)	Ece1	NP_446048.2
Danio rerio (zebrafish)	ece1	NP_001071260.1
	ECE1	XP_001099089.1
Gallus gallus (chicken)	ECE1	NP_990048.1
Canis lupus familiaris (dog)	ECE1	XP_544514.3
Drosophila melanogaster (fruit fly)	Nep3	NP_001245776.1
Homo sapiens (human)	ECE1	NP_001106820.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007796	XP_317711.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ECE1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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