CROT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CROT
Entrez Gene ID	281092
Full Name	carnitine O-octanoyltransferase
General protein information	Preferred Names carnitine O-octanoyltransferase Names peroxisomal carnitine O-octanoyltransferase COT
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024990439.1	XP_024846207.1	peroxisomal carnitine O-octanoyltransferase isoform X1
XM_024990440.1	XP_024846208.1	peroxisomal carnitine O-octanoyltransferase isoform X1
NM_177494.2	NP_803460.1	peroxisomal carnitine O-octanoyltransferase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Crot	NP_076222.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002329	XP_312640.4
Pan troglodytes (chimpanzee)	CROT	XP_001162955.1
Canis lupus familiaris (dog)	CROT	XP_539402.1
Drosophila melanogaster (fruit fly)	CG12428	NP_651583.1
Homo sapiens (human)	CROT	NP_001137407.1
Rattus norvegicus (Norway rat)	Crot	NP_114193.1
Gallus gallus (chicken)	CROT	XP_418635.2
Caenorhabditis elegans (roundworm)	CELE_T20B3.1	NP_507245.1
Bos taurus (cattle)	CROT	NP_803460.1
Danio rerio (zebrafish)	crot	NP_001018161.1
Caenorhabditis elegans (roundworm)	F41E7.6	NP_509729.2
Xenopus tropicalis (tropical clawed frog)	crot	NP_001128278.1
	CROT	XP_001106063.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids.
Ferdinandusse S, Mulders J, IJlst L, Denis S, Dacremont G, Waterham HR, Wanders RJ
Biochemical and biophysical research communications263(1)213-8(1999 Sep)

cDNA cloning, recombinant expression, and site-directed mutagenesis of bovine liver carnitine octanoyltransferase--Arg505 binds the carboxylate group of carnitine.
Cronin CN
European journal of biochemistry247(3)1029-37(1997 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CROT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CROT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01336	NM_177494.2 Latest version!	Bos taurus carnitine O-octanoyltransferase (CROT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa01336	XM_024990440.1 Latest version!	Bos taurus carnitine O-octanoyltransferase (CROT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa01336	XM_024990439.1 Latest version!	Bos taurus carnitine O-octanoyltransferase (CROT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CROT ( NM_177494.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_803460.1 Bos taurus carnitine O-octanoyltransferase (CROT), mRNA.

Price & Availability↑

CloneID	OBa01336
Clone ID Related Accession (Same CDS sequence)	XM_024990439.1 , XM_024990440.1 , NM_177494.2
Accession Version	NM_177494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	peroxisomal carnitine O-octanoyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U65745.1. On or before Oct 4, 2005 this sequence version replaced XM_865619.1, NM_177494.1. ##Evidence-Data-START## Transcript exon combination :: U65745.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGAAAATC AACTGGCTAA ATCAACTGAA GAAAGGACAT TTCAGTACCA AGATTCCCTT 
CCATCGCTGC CTGTTCCTTC ACTTGAAGAA TCATTAAAAA AATACCTTGA ATCAGTAAAG 
CCATTTGCAA ATGAGGAAGA ATATAAGAAT ACTGAAGCAA TTGTCTGGAA ATTTCAAAAT 
GGCATTGGAG AAAAATTGCA GCAGAAATTA CTTCAAAGAG CAAAAGGAAG AAGAAACTGG 
CTGGAAGAGT GGTGGCTGAA TGTTGCCTAC CTGGATGTTC GTATACCATC ACAACTGAAT 
GTCAACTTTG GGGGTCCTGC AAGCCATATT GAACACTACT GGCCTCCAAA GGAAGGCACA 
CAGTTGGAAA GAGGAAGTAT AAGTCTTTGG CATAACTTGA ACTACTGGCA GCTATTAAGA 
AAAGAAAAGT TAGCTGTTGA AAAAGTTGGA AATACTCCTC TAGATATGAA TCAATTCCGA 
ATGCTGTTTT CCACCTGCAA GATTCCAGGA ATTACTAGAG ATTCAATTAT TAATTATTTT 
AGGACTGAGA GTGAAGGACA TTCTCCAAGT CACCTTGCAG TGTTGTGTCG AGGCCGAGTT 
TTTGTCTTTG ATGTAATGCA TGAGGGATAT CTGATGACTG CACCAGAGAT TCAGAGGCAA 
TTGACATACA TCCAAAAGAA ATGCCACAGT GAACCCGACG GACCTGGGGT AGCAGCGTTA 
ACTACTGAGG AGCGAACTCG ATGGGCTAAG GCACGAGAAT ATTTGATTAG TCTTAATCCA 
GAGAACCTGA CTATATTAGA AAAAATTCAG AGCAGTTTAC TGGTATTTTG TTTAGATGAT 
GACAGTCCAC ATGTTACACC AGAAGATTAT TCTCAGGTTT CTGCAAAGAT TCTTAATGGA 
GACCCAACAG TACGCTGGGG TGACAAATCT TATAACTTGA TTGCCTTTTC TAATGGAGTA 
TTTGGCAGTA ATTGTGATCA TGCTCCTTTT GATGCAATGG TTCTGGTAAA AGTCTGCTAT 
TATGTTGATG AGAACATTCT TGAGAATGAA GGAAGATGGA AGGGCTCAGA AAAAGTACGG 
GATATACCGG TTCCAGAGGA GCTTGTTTTC ACTGTGGATG AAAAAGTATT AAATGACATC 
AACCAAGCTA AGGCCCAGTA TTTCAAGCAG GTGTCCGATC TCCAGCTTGT GGTATATGCC 
TTTACGTCTT TTGGCAAAAA GCTAACCAAG GAGAAGCAGC TTCACCCTGA TACATTTATT 
CAGCTGGCAC TTCAGTTGGC CTATTACAGA CTTCATGGAC GTCCTGGTTG CTGCTATGAA 
ACAGCTATGA CAAGATTATT TTATCATGGC CGTACAGAGA CTGTGCGACC ATGTACAGTA 
GAAGCAGTCA ATTGGTGCCA ATCCATGCAG AATCCTTCTA CCAGTCTTCT TGAGCGGAAG 
CACATGATGT TAGAAGCTTT TGCAAAACAT AATAAAATGA TGAAAGATTG TTCAACTGGA 
AAAGGATTTG ACCGTCATCT CTTAGGCCTC TCACTCATAG CAAAGGAGGA AGGCCTCCCT 
GTTCCAGAAC TCTTCACAGA CCCCCTCTTC TCCAGGAGTG GAGGAGGTGG AAACTTTGTT 
CTCTCAACCA GTCTGGTTGG TTATTTAAGA GTCCAGGGCG TGATGGTTCC CATGGTGCAC 
AATGGCTATG GATTCTTCTA TCATATCAGA GATGACAGGT TTGTTGTGTC CTGTTCAGCC 
TGGAAATCAT GTCCAGAGAC TGATGCAGAG AAGCTAGTTC AGCAGGTTTT TCATGCTTTC 
TGTGATATGA TGCAACTGAT GGAAATGCCT CATCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803460.1
CDS	339..2177
Translation	MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVKPFANEEEYKNTEAIVWKFQNGIGEKLQQKLLQRAKGRRNWLEEWWLNVAYLDVRIPSQLNVNFGGPASHIEHYWPPKEGTQLERGSISLWHNLNYWQLLRKEKLAVEKVGNTPLDMNQFRMLFSTCKIPGITRDSIINYFRTESEGHSPSHLAVLCRGRVFVFDVMHEGYLMTAPEIQRQLTYIQKKCHSEPDGPGVAALTTEERTRWAKAREYLISLNPENLTILEKIQSSLLVFCLDDDSPHVTPEDYSQVSAKILNGDPTVRWGDKSYNLIAFSNGVFGSNCDHAPFDAMVLVKVCYYVDENILENEGRWKGSEKVRDIPVPEELVFTVDEKVLNDINQAKAQYFKQVSDLQLVVYAFTSFGKKLTKEKQLHPDTFIQLALQLAYYRLHGRPGCCYETAMTRLFYHGRTETVRPCTVEAVNWCQSMQNPSTSLLERKHMMLEAFAKHNKMMKDCSTGKGFDRHLLGLSLIAKEEGLPVPELFTDPLFSRSGGGGNFVLSTSLVGYLRVQGVMVPMVHNGYGFFYHIRDDRFVVSCSAWKSCPETDAEKLVQQVFHAFCDMMQLMEMPHL

Target ORF information:

RefSeq Version	NM_177494.2
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine O-octanoyltransferase (CROT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177494.2

ORF Insert Sequence:

ATGGAAAATC AACTGGCTAA ATCAACTGAA GAAAGGACAT TTCAGTACCA AGATTCCCTT 
CCATCGCTGC CTGTTCCTTC ACTTGAAGAA TCATTAAAAA AATACCTTGA ATCAGTAAAG 
CCATTTGCAA ATGAGGAAGA ATATAAGAAT ACTGAAGCAA TTGTCTGGAA ATTTCAAAAT 
GGCATTGGAG AAAAATTGCA GCAGAAATTA CTTCAAAGAG CAAAAGGAAG AAGAAACTGG 
CTGGAAGAGT GGTGGCTGAA TGTTGCCTAC CTGGATGTTC GTATACCATC ACAACTGAAT 
GTCAACTTTG GGGGTCCTGC AAGCCATATT GAACACTACT GGCCTCCAAA GGAAGGCACA 
CAGTTGGAAA GAGGAAGTAT AAGTCTTTGG CATAACTTGA ACTACTGGCA GCTATTAAGA 
AAAGAAAAGT TAGCTGTTGA AAAAGTTGGA AATACTCCTC TAGATATGAA TCAATTCCGA 
ATGCTGTTTT CCACCTGCAA GATTCCAGGA ATTACTAGAG ATTCAATTAT TAATTATTTT 
AGGACTGAGA GTGAAGGACA TTCTCCAAGT CACCTTGCAG TGTTGTGTCG AGGCCGAGTT 
TTTGTCTTTG ATGTAATGCA TGAGGGATAT CTGATGACTG CACCAGAGAT TCAGAGGCAA 
TTGACATACA TCCAAAAGAA ATGCCACAGT GAACCCGACG GACCTGGGGT AGCAGCGTTA 
ACTACTGAGG AGCGAACTCG ATGGGCTAAG GCACGAGAAT ATTTGATTAG TCTTAATCCA 
GAGAACCTGA CTATATTAGA AAAAATTCAG AGCAGTTTAC TGGTATTTTG TTTAGATGAT 
GACAGTCCAC ATGTTACACC AGAAGATTAT TCTCAGGTTT CTGCAAAGAT TCTTAATGGA 
GACCCAACAG TACGCTGGGG TGACAAATCT TATAACTTGA TTGCCTTTTC TAATGGAGTA 
TTTGGCAGTA ATTGTGATCA TGCTCCTTTT GATGCAATGG TTCTGGTAAA AGTCTGCTAT 
TATGTTGATG AGAACATTCT TGAGAATGAA GGAAGATGGA AGGGCTCAGA AAAAGTACGG 
GATATACCGG TTCCAGAGGA GCTTGTTTTC ACTGTGGATG AAAAAGTATT AAATGACATC 
AACCAAGCTA AGGCCCAGTA TTTCAAGCAG GTGTCCGATC TCCAGCTTGT GGTATATGCC 
TTTACGTCTT TTGGCAAAAA GCTAACCAAG GAGAAGCAGC TTCACCCTGA TACATTTATT 
CAGCTGGCAC TTCAGTTGGC CTATTACAGA CTTCATGGAC GTCCTGGTTG CTGCTATGAA 
ACAGCTATGA CAAGATTATT TTATCATGGC CGTACAGAGA CTGTGCGACC ATGTACAGTA 
GAAGCAGTCA ATTGGTGCCA ATCCATGCAG AATCCTTCTA CCAGTCTTCT TGAGCGGAAG 
CACATGATGT TAGAAGCTTT TGCAAAACAT AATAAAATGA TGAAAGATTG TTCAACTGGA 
AAAGGATTTG ACCGTCATCT CTTAGGCCTC TCACTCATAG CAAAGGAGGA AGGCCTCCCT 
GTTCCAGAAC TCTTCACAGA CCCCCTCTTC TCCAGGAGTG GAGGAGGTGG AAACTTTGTT 
CTCTCAACCA GTCTGGTTGG TTATTTAAGA GTCCAGGGCG TGATGGTTCC CATGGTGCAC 
AATGGCTATG GATTCTTCTA TCATATCAGA GATGACAGGT TTGTTGTGTC CTGTTCAGCC 
TGGAAATCAT GTCCAGAGAC TGATGCAGAG AAGCTAGTTC AGCAGGTTTT TCATGCTTTC 
TGTGATATGA TGCAACTGAT GGAAATGCCT CATCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CROT ( XM_024990439.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846207.1 Bos taurus carnitine O-octanoyltransferase (CROT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa01336
Clone ID Related Accession (Same CDS sequence)	XM_024990439.1 , XM_024990440.1 , NM_177494.2
Accession Version	XM_024990439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	peroxisomal carnitine O-octanoyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC AACTGGCTAA ATCAACTGAA GAAAGGACAT TTCAGTACCA AGATTCCCTT 
CCATCGCTGC CTGTTCCTTC ACTTGAAGAA TCATTAAAAA AATACCTTGA ATCAGTAAAG 
CCATTTGCAA ATGAGGAAGA ATATAAGAAT ACTGAAGCAA TTGTCTGGAA ATTTCAAAAT 
GGCATTGGAG AAAAATTGCA GCAGAAATTA CTTCAAAGAG CAAAAGGAAG AAGAAACTGG 
CTGGAAGAGT GGTGGCTGAA TGTTGCCTAC CTGGATGTTC GTATACCATC ACAACTGAAT 
GTCAACTTTG GGGGTCCTGC AAGCCATATT GAACACTACT GGCCTCCAAA GGAAGGCACA 
CAGTTGGAAA GAGGAAGTAT AAGTCTTTGG CATAACTTGA ACTACTGGCA GCTATTAAGA 
AAAGAAAAGT TAGCTGTTGA AAAAGTTGGA AATACTCCTC TAGATATGAA TCAATTCCGA 
ATGCTGTTTT CCACCTGCAA GATTCCAGGA ATTACTAGAG ATTCAATTAT TAATTATTTT 
AGGACTGAGA GTGAAGGACA TTCTCCAAGT CACCTTGCAG TGTTGTGTCG AGGCCGAGTT 
TTTGTCTTTG ATGTAATGCA TGAGGGATAT CTGATGACTG CACCAGAGAT TCAGAGGCAA 
TTGACATACA TCCAAAAGAA ATGCCACAGT GAACCCGACG GACCTGGGGT AGCAGCGTTA 
ACTACTGAGG AGCGAACTCG ATGGGCTAAG GCACGAGAAT ATTTGATTAG TCTTAATCCA 
GAGAACCTGA CTATATTAGA AAAAATTCAG AGCAGTTTAC TGGTATTTTG TTTAGATGAT 
GACAGTCCAC ATGTTACACC AGAAGATTAT TCTCAGGTTT CTGCAAAGAT TCTTAATGGA 
GACCCAACAG TACGCTGGGG TGACAAATCT TATAACTTGA TTGCCTTTTC TAATGGAGTA 
TTTGGCAGTA ATTGTGATCA TGCTCCTTTT GATGCAATGG TTCTGGTAAA AGTCTGCTAT 
TATGTTGATG AGAACATTCT TGAGAATGAA GGAAGATGGA AGGGCTCAGA AAAAGTACGG 
GATATACCGG TTCCAGAGGA GCTTGTTTTC ACTGTGGATG AAAAAGTATT AAATGACATC 
AACCAAGCTA AGGCCCAGTA TTTCAAGCAG GTGTCCGATC TCCAGCTTGT GGTATATGCC 
TTTACGTCTT TTGGCAAAAA GCTAACCAAG GAGAAGCAGC TTCACCCTGA TACATTTATT 
CAGCTGGCAC TTCAGTTGGC CTATTACAGA CTTCATGGAC GTCCTGGTTG CTGCTATGAA 
ACAGCTATGA CAAGATTATT TTATCATGGC CGTACAGAGA CTGTGCGACC ATGTACAGTA 
GAAGCAGTCA ATTGGTGCCA ATCCATGCAG AATCCTTCTA CCAGTCTTCT TGAGCGGAAG 
CACATGATGT TAGAAGCTTT TGCAAAACAT AATAAAATGA TGAAAGATTG TTCAACTGGA 
AAAGGATTTG ACCGTCATCT CTTAGGCCTC TCACTCATAG CAAAGGAGGA AGGCCTCCCT 
GTTCCAGAAC TCTTCACAGA CCCCCTCTTC TCCAGGAGTG GAGGAGGTGG AAACTTTGTT 
CTCTCAACCA GTCTGGTTGG TTATTTAAGA GTCCAGGGCG TGATGGTTCC CATGGTGCAC 
AATGGCTATG GATTCTTCTA TCATATCAGA GATGACAGGT TTGTTGTGTC CTGTTCAGCC 
TGGAAATCAT GTCCAGAGAC TGATGCAGAG AAGCTAGTTC AGCAGGTTTT TCATGCTTTC 
TGTGATATGA TGCAACTGAT GGAAATGCCT CATCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846207.1
CDS	216..2054
Translation	MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVKPFANEEEYKNTEAIVWKFQNGIGEKLQQKLLQRAKGRRNWLEEWWLNVAYLDVRIPSQLNVNFGGPASHIEHYWPPKEGTQLERGSISLWHNLNYWQLLRKEKLAVEKVGNTPLDMNQFRMLFSTCKIPGITRDSIINYFRTESEGHSPSHLAVLCRGRVFVFDVMHEGYLMTAPEIQRQLTYIQKKCHSEPDGPGVAALTTEERTRWAKAREYLISLNPENLTILEKIQSSLLVFCLDDDSPHVTPEDYSQVSAKILNGDPTVRWGDKSYNLIAFSNGVFGSNCDHAPFDAMVLVKVCYYVDENILENEGRWKGSEKVRDIPVPEELVFTVDEKVLNDINQAKAQYFKQVSDLQLVVYAFTSFGKKLTKEKQLHPDTFIQLALQLAYYRLHGRPGCCYETAMTRLFYHGRTETVRPCTVEAVNWCQSMQNPSTSLLERKHMMLEAFAKHNKMMKDCSTGKGFDRHLLGLSLIAKEEGLPVPELFTDPLFSRSGGGGNFVLSTSLVGYLRVQGVMVPMVHNGYGFFYHIRDDRFVVSCSAWKSCPETDAEKLVQQVFHAFCDMMQLMEMPHL

Target ORF information:

RefSeq Version	XM_024990439.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine O-octanoyltransferase (CROT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990439.1

ORF Insert Sequence:

ATGGAAAATC AACTGGCTAA ATCAACTGAA GAAAGGACAT TTCAGTACCA AGATTCCCTT 
CCATCGCTGC CTGTTCCTTC ACTTGAAGAA TCATTAAAAA AATACCTTGA ATCAGTAAAG 
CCATTTGCAA ATGAGGAAGA ATATAAGAAT ACTGAAGCAA TTGTCTGGAA ATTTCAAAAT 
GGCATTGGAG AAAAATTGCA GCAGAAATTA CTTCAAAGAG CAAAAGGAAG AAGAAACTGG 
CTGGAAGAGT GGTGGCTGAA TGTTGCCTAC CTGGATGTTC GTATACCATC ACAACTGAAT 
GTCAACTTTG GGGGTCCTGC AAGCCATATT GAACACTACT GGCCTCCAAA GGAAGGCACA 
CAGTTGGAAA GAGGAAGTAT AAGTCTTTGG CATAACTTGA ACTACTGGCA GCTATTAAGA 
AAAGAAAAGT TAGCTGTTGA AAAAGTTGGA AATACTCCTC TAGATATGAA TCAATTCCGA 
ATGCTGTTTT CCACCTGCAA GATTCCAGGA ATTACTAGAG ATTCAATTAT TAATTATTTT 
AGGACTGAGA GTGAAGGACA TTCTCCAAGT CACCTTGCAG TGTTGTGTCG AGGCCGAGTT 
TTTGTCTTTG ATGTAATGCA TGAGGGATAT CTGATGACTG CACCAGAGAT TCAGAGGCAA 
TTGACATACA TCCAAAAGAA ATGCCACAGT GAACCCGACG GACCTGGGGT AGCAGCGTTA 
ACTACTGAGG AGCGAACTCG ATGGGCTAAG GCACGAGAAT ATTTGATTAG TCTTAATCCA 
GAGAACCTGA CTATATTAGA AAAAATTCAG AGCAGTTTAC TGGTATTTTG TTTAGATGAT 
GACAGTCCAC ATGTTACACC AGAAGATTAT TCTCAGGTTT CTGCAAAGAT TCTTAATGGA 
GACCCAACAG TACGCTGGGG TGACAAATCT TATAACTTGA TTGCCTTTTC TAATGGAGTA 
TTTGGCAGTA ATTGTGATCA TGCTCCTTTT GATGCAATGG TTCTGGTAAA AGTCTGCTAT 
TATGTTGATG AGAACATTCT TGAGAATGAA GGAAGATGGA AGGGCTCAGA AAAAGTACGG 
GATATACCGG TTCCAGAGGA GCTTGTTTTC ACTGTGGATG AAAAAGTATT AAATGACATC 
AACCAAGCTA AGGCCCAGTA TTTCAAGCAG GTGTCCGATC TCCAGCTTGT GGTATATGCC 
TTTACGTCTT TTGGCAAAAA GCTAACCAAG GAGAAGCAGC TTCACCCTGA TACATTTATT 
CAGCTGGCAC TTCAGTTGGC CTATTACAGA CTTCATGGAC GTCCTGGTTG CTGCTATGAA 
ACAGCTATGA CAAGATTATT TTATCATGGC CGTACAGAGA CTGTGCGACC ATGTACAGTA 
GAAGCAGTCA ATTGGTGCCA ATCCATGCAG AATCCTTCTA CCAGTCTTCT TGAGCGGAAG 
CACATGATGT TAGAAGCTTT TGCAAAACAT AATAAAATGA TGAAAGATTG TTCAACTGGA 
AAAGGATTTG ACCGTCATCT CTTAGGCCTC TCACTCATAG CAAAGGAGGA AGGCCTCCCT 
GTTCCAGAAC TCTTCACAGA CCCCCTCTTC TCCAGGAGTG GAGGAGGTGG AAACTTTGTT 
CTCTCAACCA GTCTGGTTGG TTATTTAAGA GTCCAGGGCG TGATGGTTCC CATGGTGCAC 
AATGGCTATG GATTCTTCTA TCATATCAGA GATGACAGGT TTGTTGTGTC CTGTTCAGCC 
TGGAAATCAT GTCCAGAGAC TGATGCAGAG AAGCTAGTTC AGCAGGTTTT TCATGCTTTC 
TGTGATATGA TGCAACTGAT GGAAATGCCT CATCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CROT ( XM_024990440.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846208.1 Bos taurus carnitine O-octanoyltransferase (CROT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa01336
Clone ID Related Accession (Same CDS sequence)	XM_024990439.1 , XM_024990440.1 , NM_177494.2
Accession Version	XM_024990440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	peroxisomal carnitine O-octanoyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATC AACTGGCTAA ATCAACTGAA GAAAGGACAT TTCAGTACCA AGATTCCCTT 
CCATCGCTGC CTGTTCCTTC ACTTGAAGAA TCATTAAAAA AATACCTTGA ATCAGTAAAG 
CCATTTGCAA ATGAGGAAGA ATATAAGAAT ACTGAAGCAA TTGTCTGGAA ATTTCAAAAT 
GGCATTGGAG AAAAATTGCA GCAGAAATTA CTTCAAAGAG CAAAAGGAAG AAGAAACTGG 
CTGGAAGAGT GGTGGCTGAA TGTTGCCTAC CTGGATGTTC GTATACCATC ACAACTGAAT 
GTCAACTTTG GGGGTCCTGC AAGCCATATT GAACACTACT GGCCTCCAAA GGAAGGCACA 
CAGTTGGAAA GAGGAAGTAT AAGTCTTTGG CATAACTTGA ACTACTGGCA GCTATTAAGA 
AAAGAAAAGT TAGCTGTTGA AAAAGTTGGA AATACTCCTC TAGATATGAA TCAATTCCGA 
ATGCTGTTTT CCACCTGCAA GATTCCAGGA ATTACTAGAG ATTCAATTAT TAATTATTTT 
AGGACTGAGA GTGAAGGACA TTCTCCAAGT CACCTTGCAG TGTTGTGTCG AGGCCGAGTT 
TTTGTCTTTG ATGTAATGCA TGAGGGATAT CTGATGACTG CACCAGAGAT TCAGAGGCAA 
TTGACATACA TCCAAAAGAA ATGCCACAGT GAACCCGACG GACCTGGGGT AGCAGCGTTA 
ACTACTGAGG AGCGAACTCG ATGGGCTAAG GCACGAGAAT ATTTGATTAG TCTTAATCCA 
GAGAACCTGA CTATATTAGA AAAAATTCAG AGCAGTTTAC TGGTATTTTG TTTAGATGAT 
GACAGTCCAC ATGTTACACC AGAAGATTAT TCTCAGGTTT CTGCAAAGAT TCTTAATGGA 
GACCCAACAG TACGCTGGGG TGACAAATCT TATAACTTGA TTGCCTTTTC TAATGGAGTA 
TTTGGCAGTA ATTGTGATCA TGCTCCTTTT GATGCAATGG TTCTGGTAAA AGTCTGCTAT 
TATGTTGATG AGAACATTCT TGAGAATGAA GGAAGATGGA AGGGCTCAGA AAAAGTACGG 
GATATACCGG TTCCAGAGGA GCTTGTTTTC ACTGTGGATG AAAAAGTATT AAATGACATC 
AACCAAGCTA AGGCCCAGTA TTTCAAGCAG GTGTCCGATC TCCAGCTTGT GGTATATGCC 
TTTACGTCTT TTGGCAAAAA GCTAACCAAG GAGAAGCAGC TTCACCCTGA TACATTTATT 
CAGCTGGCAC TTCAGTTGGC CTATTACAGA CTTCATGGAC GTCCTGGTTG CTGCTATGAA 
ACAGCTATGA CAAGATTATT TTATCATGGC CGTACAGAGA CTGTGCGACC ATGTACAGTA 
GAAGCAGTCA ATTGGTGCCA ATCCATGCAG AATCCTTCTA CCAGTCTTCT TGAGCGGAAG 
CACATGATGT TAGAAGCTTT TGCAAAACAT AATAAAATGA TGAAAGATTG TTCAACTGGA 
AAAGGATTTG ACCGTCATCT CTTAGGCCTC TCACTCATAG CAAAGGAGGA AGGCCTCCCT 
GTTCCAGAAC TCTTCACAGA CCCCCTCTTC TCCAGGAGTG GAGGAGGTGG AAACTTTGTT 
CTCTCAACCA GTCTGGTTGG TTATTTAAGA GTCCAGGGCG TGATGGTTCC CATGGTGCAC 
AATGGCTATG GATTCTTCTA TCATATCAGA GATGACAGGT TTGTTGTGTC CTGTTCAGCC 
TGGAAATCAT GTCCAGAGAC TGATGCAGAG AAGCTAGTTC AGCAGGTTTT TCATGCTTTC 
TGTGATATGA TGCAACTGAT GGAAATGCCT CATCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846208.1
CDS	133..1971
Translation	MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVKPFANEEEYKNTEAIVWKFQNGIGEKLQQKLLQRAKGRRNWLEEWWLNVAYLDVRIPSQLNVNFGGPASHIEHYWPPKEGTQLERGSISLWHNLNYWQLLRKEKLAVEKVGNTPLDMNQFRMLFSTCKIPGITRDSIINYFRTESEGHSPSHLAVLCRGRVFVFDVMHEGYLMTAPEIQRQLTYIQKKCHSEPDGPGVAALTTEERTRWAKAREYLISLNPENLTILEKIQSSLLVFCLDDDSPHVTPEDYSQVSAKILNGDPTVRWGDKSYNLIAFSNGVFGSNCDHAPFDAMVLVKVCYYVDENILENEGRWKGSEKVRDIPVPEELVFTVDEKVLNDINQAKAQYFKQVSDLQLVVYAFTSFGKKLTKEKQLHPDTFIQLALQLAYYRLHGRPGCCYETAMTRLFYHGRTETVRPCTVEAVNWCQSMQNPSTSLLERKHMMLEAFAKHNKMMKDCSTGKGFDRHLLGLSLIAKEEGLPVPELFTDPLFSRSGGGGNFVLSTSLVGYLRVQGVMVPMVHNGYGFFYHIRDDRFVVSCSAWKSCPETDAEKLVQQVFHAFCDMMQLMEMPHL

Target ORF information:

RefSeq Version	XM_024990440.1
Organism	Bos taurus(cattle)
Definition	Bos taurus carnitine O-octanoyltransferase (CROT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990440.1

ORF Insert Sequence:

ATGGAAAATC AACTGGCTAA ATCAACTGAA GAAAGGACAT TTCAGTACCA AGATTCCCTT 
CCATCGCTGC CTGTTCCTTC ACTTGAAGAA TCATTAAAAA AATACCTTGA ATCAGTAAAG 
CCATTTGCAA ATGAGGAAGA ATATAAGAAT ACTGAAGCAA TTGTCTGGAA ATTTCAAAAT 
GGCATTGGAG AAAAATTGCA GCAGAAATTA CTTCAAAGAG CAAAAGGAAG AAGAAACTGG 
CTGGAAGAGT GGTGGCTGAA TGTTGCCTAC CTGGATGTTC GTATACCATC ACAACTGAAT 
GTCAACTTTG GGGGTCCTGC AAGCCATATT GAACACTACT GGCCTCCAAA GGAAGGCACA 
CAGTTGGAAA GAGGAAGTAT AAGTCTTTGG CATAACTTGA ACTACTGGCA GCTATTAAGA 
AAAGAAAAGT TAGCTGTTGA AAAAGTTGGA AATACTCCTC TAGATATGAA TCAATTCCGA 
ATGCTGTTTT CCACCTGCAA GATTCCAGGA ATTACTAGAG ATTCAATTAT TAATTATTTT 
AGGACTGAGA GTGAAGGACA TTCTCCAAGT CACCTTGCAG TGTTGTGTCG AGGCCGAGTT 
TTTGTCTTTG ATGTAATGCA TGAGGGATAT CTGATGACTG CACCAGAGAT TCAGAGGCAA 
TTGACATACA TCCAAAAGAA ATGCCACAGT GAACCCGACG GACCTGGGGT AGCAGCGTTA 
ACTACTGAGG AGCGAACTCG ATGGGCTAAG GCACGAGAAT ATTTGATTAG TCTTAATCCA 
GAGAACCTGA CTATATTAGA AAAAATTCAG AGCAGTTTAC TGGTATTTTG TTTAGATGAT 
GACAGTCCAC ATGTTACACC AGAAGATTAT TCTCAGGTTT CTGCAAAGAT TCTTAATGGA 
GACCCAACAG TACGCTGGGG TGACAAATCT TATAACTTGA TTGCCTTTTC TAATGGAGTA 
TTTGGCAGTA ATTGTGATCA TGCTCCTTTT GATGCAATGG TTCTGGTAAA AGTCTGCTAT 
TATGTTGATG AGAACATTCT TGAGAATGAA GGAAGATGGA AGGGCTCAGA AAAAGTACGG 
GATATACCGG TTCCAGAGGA GCTTGTTTTC ACTGTGGATG AAAAAGTATT AAATGACATC 
AACCAAGCTA AGGCCCAGTA TTTCAAGCAG GTGTCCGATC TCCAGCTTGT GGTATATGCC 
TTTACGTCTT TTGGCAAAAA GCTAACCAAG GAGAAGCAGC TTCACCCTGA TACATTTATT 
CAGCTGGCAC TTCAGTTGGC CTATTACAGA CTTCATGGAC GTCCTGGTTG CTGCTATGAA 
ACAGCTATGA CAAGATTATT TTATCATGGC CGTACAGAGA CTGTGCGACC ATGTACAGTA 
GAAGCAGTCA ATTGGTGCCA ATCCATGCAG AATCCTTCTA CCAGTCTTCT TGAGCGGAAG 
CACATGATGT TAGAAGCTTT TGCAAAACAT AATAAAATGA TGAAAGATTG TTCAACTGGA 
AAAGGATTTG ACCGTCATCT CTTAGGCCTC TCACTCATAG CAAAGGAGGA AGGCCTCCCT 
GTTCCAGAAC TCTTCACAGA CCCCCTCTTC TCCAGGAGTG GAGGAGGTGG AAACTTTGTT 
CTCTCAACCA GTCTGGTTGG TTATTTAAGA GTCCAGGGCG TGATGGTTCC CATGGTGCAC 
AATGGCTATG GATTCTTCTA TCATATCAGA GATGACAGGT TTGTTGTGTC CTGTTCAGCC 
TGGAAATCAT GTCCAGAGAC TGATGCAGAG AAGCTAGTTC AGCAGGTTTT TCATGCTTTC 
TGTGATATGA TGCAACTGAT GGAAATGCCT CATCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids.
Biochemical and biophysical research communications263(1)213-8(1999 Sep)
Ferdinandusse S,Mulders J,IJlst L,Denis S,Dacremont G,Waterham HR,Wanders RJ

cDNA cloning, recombinant expression, and site-directed mutagenesis of bovine liver carnitine octanoyltransferase--Arg505 binds the carboxylate group of carnitine.
European journal of biochemistry247(3)1029-37(1997 Aug)
Cronin CN

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CROT cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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