CHUK cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHUK
Entrez Gene ID	281073
Full Name	conserved helix-loop-helix ubiquitous kinase
General protein information	Preferred Names conserved helix-loop-helix ubiquitous kinase Names inhibitor of nuclear factor kappa-B kinase subunit alpha I-kappa-B kinase 1 I-kappa-B kinase alpha IKK-A IKK-alpha IKK1 NFKBIKA i kappa-B kinase alpha ikBKA nuclear factor NF-kappa-B inhibitor kinase alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174021.2	NP_776446.1	inhibitor of nuclear factor kappa-B kinase subunit alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHUK	NP_001269.3
	CHUK	XP_001107171.1
Canis lupus familiaris (dog)	CHUK	XP_534990.2
Gallus gallus (chicken)	CHUK	NP_001012922.1
Bos taurus (cattle)	CHUK	NP_776446.1
Danio rerio (zebrafish)	chuk	NP_956611.1
Xenopus tropicalis (tropical clawed frog)	chuk	NP_001016900.1
Pan troglodytes (chimpanzee)	CHUK	XP_001167720.1
Mus musculus (house mouse)	Chuk	NP_031726.2
Rattus norvegicus (Norway rat)	Chuk	NP_001101058.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of the bovine IkappaB kinases (IKK)alpha and IKKbeta, the regulatory subunit NEMO and their substrate IkappaBalpha.
Rottenberg S, Schmuckli-Maurer J, Grimm S, Heussler VT, Dobbelaere DA
Gene299(1-2)293-300(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHUK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHUK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42473	NM_174021.2 Latest version!	Bos taurus component of inhibitor of nuclear factor kappa B kinase complex (CHUK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHUK ( NM_174021.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776446.1 Bos taurus component of inhibitor of nuclear factor kappa B kinase complex (CHUK), mRNA.

Price & Availability↑

CloneID	OBa42473
Clone ID Related Accession (Same CDS sequence)	NM_174021.2
Accession Version	NM_174021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ414555.1. On or before Oct 4, 2005 this sequence version replaced XM_869268.1, NM_174021.1. ##Evidence-Data-START## Transcript exon combination :: AJ414555.1, BC134510.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGGC CCCCGGGGCT GCGGCCGGGC GCGGGCGGGC CCTGGGAGAT GCGGGAGCGG 
CTGGGCACCG GCGGCTTCGG GAACGTCTGC CTGTATCAAC ATCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CCTAGAGCTA AGTACCAAAA ACAGAGAACG GTGGTGCCAT 
GAAATCCAGA TCATGAAGAA GTTGAACCAC GCCAATGTTG TAAAGGCCTG TGACGTTCCT 
GAGGAATTGA ATTTTTTGAT TAACGATGTG CCTCTGCTAG CAATGGAATA TTGTTCTGGA 
GGAGATCTCC GGAAGCTACT CAACAAACCA GAAAATTGCT GTGGACTTAA AGAAAGCCAG 
ATACTTTCTT TACTGAGTGA TATAGGATCT GGGATCCGAT ATTTGCATGA AAACAAAATT 
ATACATCGTG ATCTAAAACC TGAAAACATA GTTCTTCAGG ATGTTGGTGG AAAGATTATG 
CATAAAATAA TTGATTTGGG ATATGCCAAA GATGTTGATC AAGGAAGTCT TTGTACATCT 
TTTGTGGGAA CTTTGCAGTA TCTGGCCCCA GAGCTCTTTG AGAATAAGCC TTATACAGCC 
ACTGTTGATT ATTGGAGCTT TGGGACCATG GTGTTTGAGT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAGAAGA TTAAGAAGAA GGATCCAAAG 
TGTATATTTG CATGTGAAGA AATGACAGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAA 
CCAAATAGCC TTTGTAGTTT AATAGTAGAA CCCATGGAAA ACTGGCTGCA GTTGATGCTA 
AACTGGGATC CTCAGCAGAG AGGAGGACCT GTTGATCTTA CTTTGAAGCA GCCAAGATGT 
TTTGTATTAA TGGATCACAT TCTGAATTTG AAGATAGTAC ATATCCTAAA TATGACTTCT 
GCAAAGATAA TTTCTTTTCT GTTACCACCT GATGAAAGTC TTCATTCACT ACAGTCTCGT 
ATTGAGCGGG AAACTGGAAT AAATACTGGT TCTCAAGAGC TTCTTTCAGA GATGGGAATT 
TCCCTGGATC CTCGGAAACC AGCCTCTCAG TGTGTTCTGG ATGGAGTTAG GGGCTGTGAT 
AGCTATATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCTTCC 
AGAAGTTTAT CTGACTGTGT AAATTATATT GTACAGGACA GCAAAATACA GCTTCCAATT 
ATCCAGCTGC GTAAAGTATG GGCGGAAGCA GTACACTATG TGTCTGGGCT GAAGGAAGAC 
TACAGCAGGC TCTTTCAGGG GCAGAGAGCA GCCATGTTAA GTCTTCTTAG ATATAATACT 
AACTTGACAA AAATGAAAAA CACTTTGATC TCAGCATCTC AGCAACTGAA AGCTAAATTG 
GAGTTTTTTC ACAAAAGCAT TCAGCTTGAC TTGGAAAGAT ATAGTGAGCA GATGACTTAC 
GGGATATCCT CAGAAAAAAT GTTAAAAGCA TGGAAAGAAA TGGAAGAAAA GGCTATCCAC 
TATGCTGAGG TGGGTGTCAT TGGATACCTG GAGGATCAAA TCATGTCCCT GCACACTGAA 
ATCATGGAGC TACAGAAGAG CCCCTACGGA CGACGTCAGG GAGACTTGAT GGAATCTCTG 
GAACAGCGCG CAATTGATCT ATATAAGCAG TTAAAGCACA GACCTTCAGA TCACTCCTAC 
AGCGACAGCA CGGAGATGGT GAAGATCATT GTGCATACTG TGCAGAGTCA GGACCGGGTT 
CTCAAGGAGC TGTTTGGTCA TCTGAGCAAG TTGTTGGGCT GTAAGCAGAA GATTATTGAT 
CTACTGCCCA AGGTGGAAAT GGCCCTCAGT AACATCAAAG AAGCTGACAG TACTGTCATG 
TTTATGCAGG GGAAGAGGCA GAAAGAAATA TGGCATCTCC TTAAAATTGC CTGTACTCAG 
AGTTCTGCTC GGTCCCTTGT AGGATCCAGT CTAGAAGGTG TAACCCCTCA GCTGCCCCCA 
ACTTCAGCAG AACGTGAACA TCCTCTGTCA TGCGTGGTAA CTCCTCAAGA TGGGGAGACT 
TTAGCACAAA TGATAGAAGA AAATTTGAAC TGTCTTGGCC ATTTAAGCAC TATTATTCAT 
GAAGCAAATG AAAAACAGGG CAACAATATG ATGAGTCTTG ATTGGAGTTG GTTAACAGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776446.1
CDS	47..2269
Translation	MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIMHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSEMGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNTNLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEMALSNIKEADSTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGVTPQLPPTSAEREHPLSCVVTPQDGETLAQMIEENLNCLGHLSTIIHEANEKQGNNMMSLDWSWLTE

Target ORF information:

RefSeq Version	NM_174021.2
Organism	Bos taurus(cattle)
Definition	Bos taurus component of inhibitor of nuclear factor kappa B kinase complex (CHUK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174021.2

ORF Insert Sequence:

ATGGAGCGGC CCCCGGGGCT GCGGCCGGGC GCGGGCGGGC CCTGGGAGAT GCGGGAGCGG 
CTGGGCACCG GCGGCTTCGG GAACGTCTGC CTGTATCAAC ATCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CCTAGAGCTA AGTACCAAAA ACAGAGAACG GTGGTGCCAT 
GAAATCCAGA TCATGAAGAA GTTGAACCAC GCCAATGTTG TAAAGGCCTG TGACGTTCCT 
GAGGAATTGA ATTTTTTGAT TAACGATGTG CCTCTGCTAG CAATGGAATA TTGTTCTGGA 
GGAGATCTCC GGAAGCTACT CAACAAACCA GAAAATTGCT GTGGACTTAA AGAAAGCCAG 
ATACTTTCTT TACTGAGTGA TATAGGATCT GGGATCCGAT ATTTGCATGA AAACAAAATT 
ATACATCGTG ATCTAAAACC TGAAAACATA GTTCTTCAGG ATGTTGGTGG AAAGATTATG 
CATAAAATAA TTGATTTGGG ATATGCCAAA GATGTTGATC AAGGAAGTCT TTGTACATCT 
TTTGTGGGAA CTTTGCAGTA TCTGGCCCCA GAGCTCTTTG AGAATAAGCC TTATACAGCC 
ACTGTTGATT ATTGGAGCTT TGGGACCATG GTGTTTGAGT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAGAAGA TTAAGAAGAA GGATCCAAAG 
TGTATATTTG CATGTGAAGA AATGACAGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAA 
CCAAATAGCC TTTGTAGTTT AATAGTAGAA CCCATGGAAA ACTGGCTGCA GTTGATGCTA 
AACTGGGATC CTCAGCAGAG AGGAGGACCT GTTGATCTTA CTTTGAAGCA GCCAAGATGT 
TTTGTATTAA TGGATCACAT TCTGAATTTG AAGATAGTAC ATATCCTAAA TATGACTTCT 
GCAAAGATAA TTTCTTTTCT GTTACCACCT GATGAAAGTC TTCATTCACT ACAGTCTCGT 
ATTGAGCGGG AAACTGGAAT AAATACTGGT TCTCAAGAGC TTCTTTCAGA GATGGGAATT 
TCCCTGGATC CTCGGAAACC AGCCTCTCAG TGTGTTCTGG ATGGAGTTAG GGGCTGTGAT 
AGCTATATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCTTCC 
AGAAGTTTAT CTGACTGTGT AAATTATATT GTACAGGACA GCAAAATACA GCTTCCAATT 
ATCCAGCTGC GTAAAGTATG GGCGGAAGCA GTACACTATG TGTCTGGGCT GAAGGAAGAC 
TACAGCAGGC TCTTTCAGGG GCAGAGAGCA GCCATGTTAA GTCTTCTTAG ATATAATACT 
AACTTGACAA AAATGAAAAA CACTTTGATC TCAGCATCTC AGCAACTGAA AGCTAAATTG 
GAGTTTTTTC ACAAAAGCAT TCAGCTTGAC TTGGAAAGAT ATAGTGAGCA GATGACTTAC 
GGGATATCCT CAGAAAAAAT GTTAAAAGCA TGGAAAGAAA TGGAAGAAAA GGCTATCCAC 
TATGCTGAGG TGGGTGTCAT TGGATACCTG GAGGATCAAA TCATGTCCCT GCACACTGAA 
ATCATGGAGC TACAGAAGAG CCCCTACGGA CGACGTCAGG GAGACTTGAT GGAATCTCTG 
GAACAGCGCG CAATTGATCT ATATAAGCAG TTAAAGCACA GACCTTCAGA TCACTCCTAC 
AGCGACAGCA CGGAGATGGT GAAGATCATT GTGCATACTG TGCAGAGTCA GGACCGGGTT 
CTCAAGGAGC TGTTTGGTCA TCTGAGCAAG TTGTTGGGCT GTAAGCAGAA GATTATTGAT 
CTACTGCCCA AGGTGGAAAT GGCCCTCAGT AACATCAAAG AAGCTGACAG TACTGTCATG 
TTTATGCAGG GGAAGAGGCA GAAAGAAATA TGGCATCTCC TTAAAATTGC CTGTACTCAG 
AGTTCTGCTC GGTCCCTTGT AGGATCCAGT CTAGAAGGTG TAACCCCTCA GCTGCCCCCA 
ACTTCAGCAG AACGTGAACA TCCTCTGTCA TGCGTGGTAA CTCCTCAAGA TGGGGAGACT 
TTAGCACAAA TGATAGAAGA AAATTTGAAC TGTCTTGGCC ATTTAAGCAC TATTATTCAT 
GAAGCAAATG AAAAACAGGG CAACAATATG ATGAGTCTTG ATTGGAGTTG GTTAACAGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of the bovine IkappaB kinases (IKK)alpha and IKKbeta, the regulatory subunit NEMO and their substrate IkappaBalpha.
Gene299(1-2)293-300(2002 Oct)
Rottenberg S,Schmuckli-Maurer J,Grimm S,Heussler VT,Dobbelaere DA

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CHUK cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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