BCL2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCL2
Entrez Gene ID	281020
Full Name	BCL2, apoptosis regulator
General protein information	Preferred Names BCL2, apoptosis regulator Names apoptosis regulator Bcl-2 B-cell CLL/lymphoma 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984175.1	XP_024839943.1	apoptosis regulator Bcl-2 isoform X2
XM_005224105.4	XP_005224162.1	apoptosis regulator Bcl-2 isoform X3
XM_005224105.3	XP_005224162.1	apoptosis regulator Bcl-2 isoform X1
XM_024984176.1	XP_024839944.1	apoptosis regulator Bcl-2 isoform X4
NM_001166486.1	NP_001159958.1	apoptosis regulator Bcl-2
XM_024984174.1	XP_024839942.1	apoptosis regulator Bcl-2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCL2	NP_000624.2
Pan troglodytes (chimpanzee)	BCL2	XP_001145537.1
Canis lupus familiaris (dog)	BCL2	NP_001002949.1
Xenopus tropicalis (tropical clawed frog)	bcl2	XP_002934442.1
Bos taurus (cattle)	BCL2	NP_001159958.1
Rattus norvegicus (Norway rat)	Bcl2	NP_058689.1
Gallus gallus (chicken)	BCL2	NP_990670.1
Mus musculus (house mouse)	Bcl2	NP_033871.2

Gene Ontology
Bibliography

Related articles in PubMed

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage.
Wu LM, Guo R, Hui L, Ye YG, Xiang JM, Wan CY, Zou M, Ma R, Sun XZ, Yang SJ, Guo DZ
Journal of veterinary science15(4)475-83(2014 Dec)

Bovine herpesvirus type 5 infection regulates Bax/BCL-2 ratio.
Garcia AF, Novais JB, Antello TF, Silva-Frade C, Ferrarezi MC, Flores EF, Cardoso TC
Genetics and molecular research : GMR12(3)3897-904(2013 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Resveratrol disrupts peroxynitrite-triggered mitochondrial apoptotic pathway: a role for Bcl-2.
Brito PM, Sim?es NF, Almeida LM, Dinis TC
Apoptosis : an international journal on programmed cell death13(8)1043-53(2008 Aug)

mRNA expression of Bcl-2, Bax, caspase-3 and -7 cannot be used as a marker for apoptosis in bovine blastocysts.
Vandaele L, Goossens K, Peelman L, Van Soom A
Animal reproduction science106(1-2)168-73(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Over-expression of STC-1 up-regulated Bcl-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggest that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism.
Title: Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage.

BoHV-5 replication apparently modulates BCL-2 expression and gene transcription, enhancing production of virus progeny, but does not increase Bax expression.
Title: Bovine herpesvirus type 5 infection regulates Bax/BCL-2 ratio.

These results propose an additional mechanism whereby resveratrol may exert its cardioprotective effects and suggest a key role for Bcl-2 in the resveratrol anti-apoptotic action, especially in disrupting peroxynitrite-triggered mitochondrial pathway.
Title: Resveratrol disrupts peroxynitrite-triggered mitochondrial apoptotic pathway: a role for Bcl-2.

mRNA expression of Bax, Bcl-2, caspase-3 and-7 cannot be used as a reliable apoptosis detection method.
Title: mRNA expression of Bcl-2, Bax, caspase-3 and -7 cannot be used as a marker for apoptosis in bovine blastocysts.

apoptosis-inducing factor was expressed in luminal alveolar cells and, in concert with a change in bax protein to bcl-2 protein ratio, might contribute to signalling of a change in the dynamic balance of the cell population as lactation progresses
Title: Functional expression of bcl-2 protein family and AIF in bovine mammary tissue in early lactation.

The following BCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229844	XM_024984174.1 Latest version!	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa44522	NM_001166486.1 Latest version!	Bos taurus BCL2 apoptosis regulator (BCL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OBa32489	XM_005224105.4 Latest version!	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OBa45052	XM_024984175.1 Latest version!	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OBa45053	XM_024984176.1 Latest version!	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00
View Old Accession Versions >>
OBa32489	XM_005224105.3	Bos taurus B-cell CLL/lymphoma 2 (BCL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCL2 ( XM_024984174.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839942.1 Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229844
Clone ID Related Accession (Same CDS sequence)	XM_024984174.1
Accession Version	XM_024984174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apoptosis regulator Bcl-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGATCCT TTCACAGCTT TTACCCGGTG CCAGGCGCCG TCGGAACCTG 
GTGAATAAGA ATTGTAATTG GAGAAGAGTT GCTTTACAAG GTTGTGTTGG TTTCCGCTGT 
ACACCAACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839942.1
CDS	1157..1828
Translation	MAHAGGTGYDNREIVMKYIHYKLSQRGYEWDAGDAGAAPPGAAPAPGILSSQPGRTPAPSRTSPPPPPAAAAGPAPSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDSIALWMTEYLNRHLHTWIQDNGGWILSQLLPGARRRRNLVNKNCNWRRVALQGCVGFRCTPT

Target ORF information:

RefSeq Version	XM_024984174.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984174.1

ORF Insert Sequence:

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGATCCT TTCACAGCTT TTACCCGGTG CCAGGCGCCG TCGGAACCTG 
GTGAATAAGA ATTGTAATTG GAGAAGAGTT GCTTTACAAG GTTGTGTTGG TTTCCGCTGT 
ACACCAACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCL2 ( NM_001166486.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001159958.1 Bos taurus BCL2 apoptosis regulator (BCL2), mRNA.

Price & Availability↑

CloneID	OBa44522
Clone ID Related Accession (Same CDS sequence)	NM_001166486.1
Accession Version	NM_001166486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Bos taurus(cattle)
Product	apoptosis regulator Bcl-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000024.1. On Oct 24, 2009 this sequence version replaced XM_586976.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U92434.1, DN543543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGGACGC CTTTGTGGAG CTGTATGGCC CTAGCATGCG GCCCCTGTTT 
GATTTCTCCT GGCTGTCTCT GAAGGCACTG CTCAGTCTGG CCCTGGTGGG CGCTTGCATC 
ACCCTGGGTG CCTATCTGGG CCATAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159958.1
CDS	1..690
Translation	MAHAGGTGYDNREIVMKYIHYKLSQRGYEWDAGDAGAAPPGAAPAPGILSSQPGRTPAPSRTSPPPPPAAAAGPAPSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDSIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKALLSLALVGACITLGAYLGHK

Target ORF information:

RefSeq Version	NM_001166486.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BCL2 apoptosis regulator (BCL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166486.1

ORF Insert Sequence:

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGGACGC CTTTGTGGAG CTGTATGGCC CTAGCATGCG GCCCCTGTTT 
GATTTCTCCT GGCTGTCTCT GAAGGCACTG CTCAGTCTGG CCCTGGTGGG CGCTTGCATC 
ACCCTGGGTG CCTATCTGGG CCATAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCL2 ( XM_005224105.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224162.1 Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa32489
Clone ID Related Accession (Same CDS sequence)	XM_005224105.4 , XM_005224105.3
Accession Version	XM_005224105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apoptosis regulator Bcl-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGTCTCC CGAAAACTGC TGCTCTCGAG GCCCCAGAAG AGATGACTCG 
GGCAAACCGA ACCCGCGGCA GGACGCGGAG CAGCCTGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224162.1
CDS	1157..1798
Translation	MAHAGGTGYDNREIVMKYIHYKLSQRGYEWDAGDAGAAPPGAAPAPGILSSQPGRTPAPSRTSPPPPPAAAAGPAPSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDSIALWMTEYLNRHLHTWIQDNGGWSPENCCSRGPRRDDSGKPNPRQDAEQPV

Target ORF information:

RefSeq Version	XM_005224105.4
Organism	Bos taurus(cattle)
Definition	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224105.4

ORF Insert Sequence:

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGTCTCC CGAAAACTGC TGCTCTCGAG GCCCCAGAAG AGATGACTCG 
GGCAAACCGA ACCCGCGGCA GGACGCGGAG CAGCCTGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCL2 ( XM_005224105.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224162.1 Bos taurus B-cell CLL/lymphoma 2 (BCL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32489
Clone ID Related Accession (Same CDS sequence)	XM_005224105.4 , XM_005224105.3
Accession Version	XM_005224105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	apoptosis regulator Bcl-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGTCTCC CGAAAACTGC TGCTCTCGAG GCCCCAGAAG AGATGACTCG 
GGCAAACCGA ACCCGCGGCA GGACGCGGAG CAGCCTGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224162.1
CDS	1167..1808
Translation	MAHAGGTGYDNREIVMKYIHYKLSQRGYEWDAGDAGAAPPGAAPAPGILSSQPGRTPAPSRTSPPPPPAAAAGPAPSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDSIALWMTEYLNRHLHTWIQDNGGWSPENCCSRGPRRDDSGKPNPRQDAEQPV

Target ORF information:

RefSeq Version	XM_005224105.3
Organism	Bos taurus(cattle)
Definition	Bos taurus B-cell CLL/lymphoma 2 (BCL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224105.3

ORF Insert Sequence:

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGTCTCC CGAAAACTGC TGCTCTCGAG GCCCCAGAAG AGATGACTCG 
GGCAAACCGA ACCCGCGGCA GGACGCGGAG CAGCCTGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCL2 ( XM_024984175.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839943.1 Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45052
Clone ID Related Accession (Same CDS sequence)	XM_024984175.1
Accession Version	XM_024984175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apoptosis regulator Bcl-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGAGAGC TCTCCCTGCC AACCCTACCG TGATCACTCC TTCCCTGGAG 
ACATTGCTTC CCTTGAGCCA GCCACTGAGG TCCAAAGATC CAGCCTTTGC TGCTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839943.1
CDS	1157..1816
Translation	MAHAGGTGYDNREIVMKYIHYKLSQRGYEWDAGDAGAAPPGAAPAPGILSSQPGRTPAPSRTSPPPPPAAAAGPAPSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDSIALWMTEYLNRHLHTWIQDNGGWRALPANPTVITPSLETLLPLSQPLRSKDPAFAAQ

Target ORF information:

RefSeq Version	XM_024984175.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984175.1

ORF Insert Sequence:

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGAGAGC TCTCCCTGCC AACCCTACCG TGATCACTCC TTCCCTGGAG 
ACATTGCTTC CCTTGAGCCA GCCACTGAGG TCCAAAGATC CAGCCTTTGC TGCTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCL2 ( XM_024984176.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839944.1 Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X4, mRNA.

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CloneID	OBa45053
Clone ID Related Accession (Same CDS sequence)	XM_024984176.1
Accession Version	XM_024984176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apoptosis regulator Bcl-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839944.1
CDS	1157..1717
Translation	MAHAGGTGYDNREIVMKYIHYKLSQRGYEWDAGDAGAAPPGAAPAPGILSSQPGRTPAPSRTSPPPPPAAAAGPAPSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDSIALWMTEYLNRHLHTWIQDNGGWV

Target ORF information:

RefSeq Version	XM_024984176.1
Organism	Bos taurus(cattle)
Definition	Bos taurus BCL2, apoptosis regulator (BCL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984176.1

ORF Insert Sequence:

ATGGCGCACG CGGGGGGAAC AGGCTACGAT AACCGAGAGA TCGTGATGAA GTACATCCAC 
TATAAGCTGT CGCAGCGGGG CTACGAGTGG GATGCCGGAG ACGCGGGCGC CGCGCCCCCC 
GGGGCCGCTC CCGCGCCGGG CATCCTGTCC TCCCAGCCGG GCCGCACACC CGCGCCCTCC 
AGGACCTCCC CGCCGCCGCC CCCGGCCGCC GCCGCCGGGC CTGCGCCCAG CCCGGTGCCG 
CCTGTGGTGC ACCTGACCCT GCGCCAGGCC GGCGATGACT TCTCTCGGCG CTACCGCCGC 
GACTTCGCCG AGATGTCCAG TCAGCTGCAC CTGACGCCCT TCACCGCGAG GGGACGCTTC 
GCCACGGTGG TGGAGGAGCT CTTCAGGGAC GGGGTGAACT GGGGGCGCAT CGTGGCCTTC 
TTTGAGTTCG GAGGGGTCAT GTGTGTGGAG AGCGTCAACC GGGAGATGTC GCCCCTGGTG 
GACAGCATCG CCCTGTGGAT GACCGAGTAC CTGAACCGGC ACCTGCACAC CTGGATCCAG 
GACAACGGAG GCTGGGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage.
Journal of veterinary science15(4)475-83(2014 Dec)
Wu LM,Guo R,Hui L,Ye YG,Xiang JM,Wan CY,Zou M,Ma R,Sun XZ,Yang SJ,Guo DZ

Bovine herpesvirus type 5 infection regulates Bax/BCL-2 ratio.
Genetics and molecular research : GMR12(3)3897-904(2013 Sep)
Garcia AF,Novais JB,Antello TF,Silva-Frade C,Ferrarezi MC,Flores EF,Cardoso TC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Resveratrol disrupts peroxynitrite-triggered mitochondrial apoptotic pathway: a role for Bcl-2.
Apoptosis : an international journal on programmed cell death13(8)1043-53(2008 Aug)
Brito PM,Sim?es NF,Almeida LM,Dinis TC

mRNA expression of Bcl-2, Bax, caspase-3 and -7 cannot be used as a marker for apoptosis in bovine blastocysts.
Animal reproduction science106(1-2)168-73(2008 Jun)
Vandaele L,Goossens K,Peelman L,Van Soom A

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BCL2 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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