AQP4 cDNA ORF clone, Bos taurus(cattle)

The following AQP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AQP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa32459 NM_001317794.2
Latest version!
Bos taurus aquaporin 4 (AQP4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa43710 NM_181003.4
Latest version!
Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa32459 XM_005224064.4
Latest version!
Bos taurus aquaporin 4 (AQP4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa134020 NM_001317792.2
Latest version!
Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
View Old Accession Versions >>
OBa134020 NM_001317792.1 Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OBa32459 XM_005224064.3 Bos taurus aquaporin 4 (AQP4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa43710 NM_181003.3 Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa32459 NM_001317794.1 Bos taurus aquaporin 4 (AQP4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa32459
    Clone ID Related Accession (Same CDS sequence) XM_005224064.3 , XM_005224064.4 , NM_001317794.1 , NM_001317794.2
    Accession Version NM_001317794.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-20
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform M23
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH131563.1, AB028642.1, DY146297.1, DAAA02056529.1 and EV702155.1. Transcript Variant: This variant (2, also known as bAQP4-B) contains an alternate 5' terminal exon, which causes translation initiation from an in-frame, downstream start codon, compared to variant 1. The resulting isoform (M23) has a shorter N-terminus compared to isoform M1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB028642.1, AB012950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304723.1
    CDS101..1006
    Translation

    Target ORF information:

    RefSeq Version NM_001317794.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32459
    Clone ID Related Accession (Same CDS sequence) XM_005224064.3 , XM_005224064.4 , NM_001317794.1 , NM_001317794.2
    Accession Version NM_001317794.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH131563.1, AB028642.1, DV840623.1, NKLS02000024.1 and EV702155.1. On Jun 13, 2018 this sequence version replaced NM_001317794.1. Transcript Variant: This variant (2, also known as bAQP4-B) contains an alternate 5' terminal exon, which causes translation initiation from an in-frame, downstream start codon, compared to variant 1. The resulting isoform (B) has a shorter N-terminus compared to isoform A. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571258.2157.1, SRR5571265.33377.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304723.1
    CDS101..1006
    Translation

    Target ORF information:

    RefSeq Version NM_001317794.2
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317794.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa43710
    Clone ID Related Accession (Same CDS sequence) NM_181003.3 , NM_181003.4
    Accession Version NM_181003.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-20
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform M1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB015947.2, DY146297.1, DAAA02056529.1 and EV702155.1. On Dec 8, 2015 this sequence version replaced NM_181003.2. Transcript Variant: This variant (1, also known as bAQP4-A) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (M1) results from translation termination at the upstream UGA stop codon, while the longer isoform (M1x) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the shorter isoform (M1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB015947.2, DV828469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851346.1
    CDS71..1042
    Translation

    Target ORF information:

    RefSeq Version NM_181003.3
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181003.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa43710
    Clone ID Related Accession (Same CDS sequence) NM_181003.3 , NM_181003.4
    Accession Version NM_181003.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-22
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB015947.2, DV840623.1, NKLS02000024.1 and EV702155.1. On Jun 13, 2018 this sequence version replaced NM_181003.3. Transcript Variant: This variant (1, also known as bAQP4-A) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (A) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ax) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the shorter isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC118415.1, SRR5571258.9507.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851346.1
    CDS71..1042
    Translation

    Target ORF information:

    RefSeq Version NM_181003.4
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181003.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32459
    Clone ID Related Accession (Same CDS sequence) XM_005224064.3 , XM_005224064.4 , NM_001317794.1 , NM_001317794.2
    Accession Version XM_005224064.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224121.1
    CDS538..1443
    Translation

    Target ORF information:

    RefSeq Version XM_005224064.3
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224064.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32459
    Clone ID Related Accession (Same CDS sequence) XM_005224064.3 , XM_005224064.4 , NM_001317794.1 , NM_001317794.2
    Accession Version XM_005224064.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224064.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224121.1
    CDS532..1437
    Translation

    Target ORF information:

    RefSeq Version XM_005224064.4
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224064.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTGGCCT TCAAAGGGGT CTGGACACAA ACTTTCTGGA AGGCAGTCAC TGCGGAATTT 
    CTGGCCATGC TTATTTTTGT CCTGCTCAGC CTGGGATCCA CCATCAACTG GGGTGGAGCA
    GAAAAGCCAC TACCTGTCGA CATGGTTCTC ATCTCCCTCT GCTTTGGACT CAGCATCGCG
    ACTATGGTCC AGTGCTTTGG CCACATCAGT GGTGGCCACA TCAACCCCGC TGTGACGGTG
    GCCATGGTGT GCACCAGGAG GATTAGCATC GCCAAGTCTG TCTTCTACAT TGCAGCTCAG
    TGCCTGGGCG CCATCATTGG AGCCGGAATT CTCTACCTGG TCACGCCTCC CAGTGTGGTA
    GGGGGCCTTG GAGTCACCAC GGTTCACGGA AATCTTAGCG CTGGACACGG TCTCCTGGTG
    GAGTTGATAA TCACATTTCA GTTGGTGTTT ACTATTTTTG CCAGCTGTGA TTCCAAACGG
    ACTGATGTCA CCGGTTCCAT AGCTTTAGCC ATTGGGATCT CTGTTGCAAT TGGACATTTA
    TTTGCAATCA ATTACACTGG TGCCAGCATG AACCCCGCCC GATCCTTTGG ACCTGCAGTT
    ATCATGGGAA ATTGGGAGAA CCACTGGATA TATTGGGTTG GACCAATCAT AGGAGCTGTC
    CTTGCTGGTG GCCTCTATGA GTATGTCTTC TGTCCAGATG TTGAACTCAA ACGTCGATTT
    AAAGAAGCCT TCAGCAAAGC TGCCCAGCAA ACAAAAGGGA GCTACATGGA GGTGGAGGAC
    AACAGGAGTC AGGTAGAGAC CGACGACTTG ATTCTAAAAC CCGGAGTGGT GCACGTGATC
    GACATTGACC GGGGGGAGGA GAAGAAAGGG AAAGACCCAT CTGGAGAGGT GTTGTCTTCA
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa134020
    Clone ID Related Accession (Same CDS sequence) NM_001317792.2 , NM_001317792.1
    Accession Version NM_001317792.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform Ax
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB015947.2, DV840623.1, NKLS02000024.1 and EV702155.1. On Jun 13, 2018 this sequence version replaced NM_001317792.1. Transcript Variant: This variant (1, also known as bAQP4-A) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (A) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ax) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the longer, C-terminally extended isoform (Ax). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC118415.1, SRR5571258.9507.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GACTAGCAGG CAGCACTGAA AGCAGACAAG AGCCCTTAGA ACTGTCCTCA
    GATCTCCTTC CACCCATTAA GGAAACAGAT TTGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304721.1
    CDS71..1129
    Translation

    Target ORF information:

    RefSeq Version NM_001317792.2
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317792.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GACTAGCAGG CAGCACTGAA AGCAGACAAG AGCCCTTAGA ACTGTCCTCA
    GATCTCCTTC CACCCATTAA GGAAACAGAT TTGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa134020
    Clone ID Related Accession (Same CDS sequence) NM_001317792.2 , NM_001317792.1
    Accession Version NM_001317792.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-20
    Organism Bos taurus(cattle)
    Product aquaporin-4 isoform M1x
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB015947.2, DY146297.1, DAAA02056529.1 and EV702155.1. Transcript Variant: This variant (1, also known as bAQP4-A) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (M1) results from translation termination at the upstream UGA stop codon, while the longer isoform (M1x) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the longer, C-terminally extended isoform (M1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB015947.2, DV828469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GACTAGCAGG CAGCACTGAA AGCAGACAAG AGCCCTTAGA ACTGTCCTCA
    GATCTCCTTC CACCCATTAA GGAAACAGAT TTGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304721.1
    CDS71..1129
    Translation

    Target ORF information:

    RefSeq Version NM_001317792.1
    Organism Bos taurus(cattle)
    Definition Bos taurus aquaporin 4 (AQP4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGTGACA GACCCGCAGC GAGGCGGTGG GGTAAGTGTG GACCTTTGTG TACAAGAGAG 
    AGTATCATGG TGGCCTTCAA AGGGGTCTGG ACACAAACTT TCTGGAAGGC AGTCACTGCG
    GAATTTCTGG CCATGCTTAT TTTTGTCCTG CTCAGCCTGG GATCCACCAT CAACTGGGGT
    GGAGCAGAAA AGCCACTACC TGTCGACATG GTTCTCATCT CCCTCTGCTT TGGACTCAGC
    ATCGCGACTA TGGTCCAGTG CTTTGGCCAC ATCAGTGGTG GCCACATCAA CCCCGCTGTG
    ACGGTGGCCA TGGTGTGCAC CAGGAGGATT AGCATCGCCA AGTCTGTCTT CTACATTGCA
    GCTCAGTGCC TGGGCGCCAT CATTGGAGCC GGAATTCTCT ACCTGGTCAC GCCTCCCAGT
    GTGGTAGGGG GCCTTGGAGT CACCACGGTT CACGGAAATC TTAGCGCTGG ACACGGTCTC
    CTGGTGGAGT TGATAATCAC ATTTCAGTTG GTGTTTACTA TTTTTGCCAG CTGTGATTCC
    AAACGGACTG ATGTCACCGG TTCCATAGCT TTAGCCATTG GGATCTCTGT TGCAATTGGA
    CATTTATTTG CAATCAATTA CACTGGTGCC AGCATGAACC CCGCCCGATC CTTTGGACCT
    GCAGTTATCA TGGGAAATTG GGAGAACCAC TGGATATATT GGGTTGGACC AATCATAGGA
    GCTGTCCTTG CTGGTGGCCT CTATGAGTAT GTCTTCTGTC CAGATGTTGA ACTCAAACGT
    CGATTTAAAG AAGCCTTCAG CAAAGCTGCC CAGCAAACAA AAGGGAGCTA CATGGAGGTG
    GAGGACAACA GGAGTCAGGT AGAGACCGAC GACTTGATTC TAAAACCCGG AGTGGTGCAC
    GTGATCGACA TTGACCGGGG GGAGGAGAAG AAAGGGAAAG ACCCATCTGG AGAGGTGTTG
    TCTTCAGTAT GACTAGCAGG CAGCACTGAA AGCAGACAAG AGCCCTTAGA ACTGTCCTCA
    GATCTCCTTC CACCCATTAA GGAAACAGAT TTGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.