APOE cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	APOE
Entrez Gene ID	281004
Full Name	apolipoprotein E
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 18 Map Location: 18q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_005219148.4	XP_005219205.2	apolipoprotein E isoform X1
XM_005219148.3	XP_005219205.1	apolipoprotein E isoform X1
XM_024977930.1	XP_024833698.1	apolipoprotein E isoform X2
NM_173991.2	NP_776416.1	apolipoprotein E precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APOE	NP_001009007.1
	APOE	XP_001104482.2
Mus musculus (house mouse)	Apoe	NP_033826.2
Canis lupus familiaris (dog)	APOE	XP_866043.1
Bos taurus (cattle)	APOE	NP_776416.1
Rattus norvegicus (Norway rat)	Apoe	NP_620183.2
Xenopus tropicalis (tropical clawed frog)	apoe	XP_004912063.1
Homo sapiens (human)	APOE	NP_000032.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro.
Liu L, Li X, Li Y, Guan Y, Song Y, Yin L, Chen H, Lei L, Liu J, Li X, Wang Z, Yang X, Liu G
Journal of dairy science97(3)1328-35(2014 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Abundance of mRNA of apolipoprotein b100, apolipoprotein e, and microsomal triglyceride transfer protein in liver from periparturient dairy cows.
Bernabucci U, Ronchi B, Basiric? L, Pirazzi D, Rueca F, Lacetera N, Nardone A
Journal of dairy science87(9)2881-8(2004 Sep)

Distribution of apolipoprotein E among lipoprotein fractions in the lactating cow.
Takahashi Y, Itoh F, Oohashi T, Miyamoto T
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology136(4)905-12(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nonesterified fatty acids significantly inhibit the expression of ApoB100, ApoE, MTP, and LDLR, thereby decreasing the synthesis and assembly of VLDL and inducing TG accumulation in bovine hepatocytes.
Title: Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro.

after calving the apolipoprotein B(100) mRNA synthesis was lower, whereas microsomal triglyceride transfer protein (MTP) and apolipoprotein E messenger RNA abundance were higher in the liver
Title: Abundance of mRNA of apolipoprotein b100, apolipoprotein e, and microsomal triglyceride transfer protein in liver from periparturient dairy cows.

apoE-containing particles, which increased during the lactating stage, were not associated with HDL particles, and lipid-free forms were included in cow plasma
Title: Distribution of apolipoprotein E among lipoprotein fractions in the lactating cow.

Bovine apoE contents in triglyceride-rich lipoproteins are modulated by nutritional treatment and closely associated with triglyceride-rich lipoprotein metabolism
Title: Bovine apolipoprotein E in plasma: increase of ApoE concentration induced by fasting and distribution in lipoprotein fractions.

The following APOE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APOE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41447	NM_173991.2 Latest version!	Bos taurus apolipoprotein E (APOE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa28800	XM_005219148.3 Latest version!	Bos taurus apolipoprotein E (APOE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa224776	XM_005219148.4 Latest version!	Bos taurus apolipoprotein E (APOE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa28801	XM_024977930.1 Latest version!	Bos taurus apolipoprotein E (APOE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APOE ( NM_173991.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776416.1 Bos taurus apolipoprotein E (APOE), mRNA.

Price & Availability↑

CloneID	OBa41447
Clone ID Related Accession (Same CDS sequence)	NM_173991.2
Accession Version	NM_173991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	apolipoprotein E precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X64839.1. On Jun 3, 2003 this sequence version replaced NM_173991.1. ##Evidence-Data-START## Transcript exon combination :: X64839.1, BC102620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTTC TGTGGGTTGC CGTGGTGGTC GCGCTTCTGG CAGGATGCCA GGCGGATATG 
GAGGGAGAGC TGGGGCCCGA GGAGCCCCTG ACTACGCAGC AGCCCCGGGG GAAGGACAGC 
CAGCCTTGGG AGCAGGCGCT GGGCCGCTTC TGGGATTACC TGCGCTGGGT GCAGACCCTG 
TCTGACCAGG TGCAGGAGGA GCTGCTCAAC ACCCAGGTCA TTCAGGAACT GACGGCGCTG 
ATGGAGGAGA CCATGAAGGA GGTGAAGGCC TACAAGGAGG AGCTGGAGGG ACAGCTAGGC 
CCCATGGCCC AGGAGACACA GGCCCGCGTG TCCAAGGAGC TGCAGGCAGC CCAGGCCCGG 
CTAGGCTCCG ACATGGAGGA CTTGCGGAAC CGCCTGGCAC AGTACCGAAG CGAGGTGCAG 
GCCATGCTGG GCCAGTCTAC CGAGGAGCTG CGGGCCCGCA TGGCCTCCCA CCTGCGCAAG 
CTGCCGAAGC GGCTGCTCCG CGACGCTGAC GACCTGAAGA AGCGCCTGGC CGTCTACCAA 
GCTGGGGCCA GCGAGGGTGC CGAGCGCAGC TTGAGCGCCA TCCGCGAGCG CTTCGGGCCC 
CTGGTGGAGC AGGGCCAATC GCGGGCAGCC ACCCTGAGCA CCCTGGCCGG CCAGCCGCTG 
CTGGAGCGTG CCGAGGCCTG GCGCCAGAAG CTGCACGGGC GCCTGGAGGA GGTGGGCGTC 
CGGGCCCAGG ACCGCCTGGA TAAGATACGC CAGCAGCTAG AGGAGGTGCA CGCCAAGGTC 
GAGGAGCAGG GCAACCAGAT GCGCCTGCAG GCCGAGGCAT TCCAGGCCCG CCTCAGGAGC 
TGGTTCGAGC CCCTGGTGGA AGACATGCAG CGCCAGTGGG CTGGGCTGGT GGAGAAGGTG 
CAGTTGGCTC TGCGCCCCAG CCCCACCTCT CCGCCCAGTG AGAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776416.1
CDS	64..1014
Translation	MKVLWVAVVVALLAGCQADMEGELGPEEPLTTQQPRGKDSQPWEQALGRFWDYLRWVQTLSDQVQEELLNTQVIQELTALMEETMKEVKAYKEELEGQLGPMAQETQARVSKELQAAQARLGSDMEDLRNRLAQYRSEVQAMLGQSTEELRARMASHLRKLPKRLLRDADDLKKRLAVYQAGASEGAERSLSAIRERFGPLVEQGQSRAATLSTLAGQPLLERAEAWRQKLHGRLEEVGVRAQDRLDKIRQQLEEVHAKVEEQGNQMRLQAEAFQARLRSWFEPLVEDMQRQWAGLVEKVQLALRPSPTSPPSENH

Target ORF information:

RefSeq Version	NM_173991.2
Organism	Bos taurus(cattle)
Definition	Bos taurus apolipoprotein E (APOE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173991.2

ORF Insert Sequence:

ATGAAGGTTC TGTGGGTTGC CGTGGTGGTC GCGCTTCTGG CAGGATGCCA GGCGGATATG 
GAGGGAGAGC TGGGGCCCGA GGAGCCCCTG ACTACGCAGC AGCCCCGGGG GAAGGACAGC 
CAGCCTTGGG AGCAGGCGCT GGGCCGCTTC TGGGATTACC TGCGCTGGGT GCAGACCCTG 
TCTGACCAGG TGCAGGAGGA GCTGCTCAAC ACCCAGGTCA TTCAGGAACT GACGGCGCTG 
ATGGAGGAGA CCATGAAGGA GGTGAAGGCC TACAAGGAGG AGCTGGAGGG ACAGCTAGGC 
CCCATGGCCC AGGAGACACA GGCCCGCGTG TCCAAGGAGC TGCAGGCAGC CCAGGCCCGG 
CTAGGCTCCG ACATGGAGGA CTTGCGGAAC CGCCTGGCAC AGTACCGAAG CGAGGTGCAG 
GCCATGCTGG GCCAGTCTAC CGAGGAGCTG CGGGCCCGCA TGGCCTCCCA CCTGCGCAAG 
CTGCCGAAGC GGCTGCTCCG CGACGCTGAC GACCTGAAGA AGCGCCTGGC CGTCTACCAA 
GCTGGGGCCA GCGAGGGTGC CGAGCGCAGC TTGAGCGCCA TCCGCGAGCG CTTCGGGCCC 
CTGGTGGAGC AGGGCCAATC GCGGGCAGCC ACCCTGAGCA CCCTGGCCGG CCAGCCGCTG 
CTGGAGCGTG CCGAGGCCTG GCGCCAGAAG CTGCACGGGC GCCTGGAGGA GGTGGGCGTC 
CGGGCCCAGG ACCGCCTGGA TAAGATACGC CAGCAGCTAG AGGAGGTGCA CGCCAAGGTC 
GAGGAGCAGG GCAACCAGAT GCGCCTGCAG GCCGAGGCAT TCCAGGCCCG CCTCAGGAGC 
TGGTTCGAGC CCCTGGTGGA AGACATGCAG CGCCAGTGGG CTGGGCTGGT GGAGAAGGTG 
CAGTTGGCTC TGCGCCCCAG CCCCACCTCT CCGCCCAGTG AGAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APOE ( XM_005219148.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219205.1 Bos taurus apolipoprotein E (APOE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28800
Clone ID Related Accession (Same CDS sequence)	XM_005219148.3
Accession Version	XM_005219148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	apolipoprotein E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGATA GCGAAAAGCC ACTGTTACTG CCGTTTATTG AGCACCTACT GGGTGCCCAC 
AATCGTCCTA AGATCGGTTG GCCAATCGCA AGCCAGAAGA TGAAGGTTCT GTGGGTTGCC 
GTGGTGGTCG CGCTTCTGGC AGGATGCCAG GCGGATATGG AGGGAGAGCT GGGGCCCGAG 
GAGCCCCTGA CTACCCAGCA GCCCCGGGGG AAGGACAGCC AGCCTTGGGA GCAGGCGCTG 
GGCCGCTTCT GGGATTACCT GCGCTGGGTG CAGACCCTGT CTGACCAGGT GCAGGAGGAG 
CTGCTCAACA CCCAGGTCAT TCAGGAACTG ACGGCGCTGA TGGAGGAGAC CATGAAGGAG 
GTGAAGGCCT ACAAGGAGGA GCTGGAGGGA CAGCTAGGCC CCATGGCCCA GGAGACACAG 
GCCCGCGTGT CCAAGGAGCT GCAGGCAGCC CAGGCCCGGC TAGGCTCCGA CATGGAGGAC 
TTGCGGAACC GCCTGGCACA GTACCGAAGC GAGGTGCAGG CCATGCTGGG CCAGTCCACC 
GAGGAGCTGC GGGCCCGCAT GGCCTCCCAC CTGCGCAAGC TGCGCAAGCG GCTGCTCCGC 
GACGCTGACG ACCTGAAGAA GCGCCTGGCC GTCTACCAAG CTGGGGCCAG CGAGGGTGCC 
GAGCGCAGCT TGAGCGCCAT CCGCGAGCGC TTCGGGCCCC TGGTGGAGCA GGGCCAATCG 
CGGGCAGCCA CCCTGAGCAC CCTGGCCGGC CAGCCGCTGC TGGAGCGTGC CGAGGCCTGG 
CGCCAGAAGC TGCACGGGCG CCTGGAGGAG GTGGGCGTCC GGGCCCAGGA CCGCCTGGAT 
AAGATACGCC AGCAGCTAGA GGAGGTGCAC GCCAAGGTCG AGGAGCAGGG CAACCAGATG 
CGCCTGCAGG CCGAGGCATT CCAGGCCCGC CTCAGGAGCT GGTTCGAGCC CCTGGTGGAA 
GACATGCAGC GCCAGTGGGC TGGGCTGGTG GAGAAGGTGC AGTTGGCTCT GCGCCCCAGC 
CCCACCTCTC CGCCCAGTGA GAATCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219205.1
CDS	249..1298
Translation	MRDSEKPLLLPFIEHLLGAHNRPKIGWPIASQKMKVLWVAVVVALLAGCQADMEGELGPEEPLTTQQPRGKDSQPWEQALGRFWDYLRWVQTLSDQVQEELLNTQVIQELTALMEETMKEVKAYKEELEGQLGPMAQETQARVSKELQAAQARLGSDMEDLRNRLAQYRSEVQAMLGQSTEELRARMASHLRKLRKRLLRDADDLKKRLAVYQAGASEGAERSLSAIRERFGPLVEQGQSRAATLSTLAGQPLLERAEAWRQKLHGRLEEVGVRAQDRLDKIRQQLEEVHAKVEEQGNQMRLQAEAFQARLRSWFEPLVEDMQRQWAGLVEKVQLALRPSPTSPPSENH

Target ORF information:

RefSeq Version	XM_005219148.3
Organism	Bos taurus(cattle)
Definition	Bos taurus apolipoprotein E (APOE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219148.3

ORF Insert Sequence:

ATGAGAGATA GCGAAAAGCC ACTGTTACTG CCGTTTATTG AGCACCTACT GGGTGCCCAC 
AATCGTCCTA AGATCGGTTG GCCAATCGCA AGCCAGAAGA TGAAGGTTCT GTGGGTTGCC 
GTGGTGGTCG CGCTTCTGGC AGGATGCCAG GCGGATATGG AGGGAGAGCT GGGGCCCGAG 
GAGCCCCTGA CTACCCAGCA GCCCCGGGGG AAGGACAGCC AGCCTTGGGA GCAGGCGCTG 
GGCCGCTTCT GGGATTACCT GCGCTGGGTG CAGACCCTGT CTGACCAGGT GCAGGAGGAG 
CTGCTCAACA CCCAGGTCAT TCAGGAACTG ACGGCGCTGA TGGAGGAGAC CATGAAGGAG 
GTGAAGGCCT ACAAGGAGGA GCTGGAGGGA CAGCTAGGCC CCATGGCCCA GGAGACACAG 
GCCCGCGTGT CCAAGGAGCT GCAGGCAGCC CAGGCCCGGC TAGGCTCCGA CATGGAGGAC 
TTGCGGAACC GCCTGGCACA GTACCGAAGC GAGGTGCAGG CCATGCTGGG CCAGTCCACC 
GAGGAGCTGC GGGCCCGCAT GGCCTCCCAC CTGCGCAAGC TGCGCAAGCG GCTGCTCCGC 
GACGCTGACG ACCTGAAGAA GCGCCTGGCC GTCTACCAAG CTGGGGCCAG CGAGGGTGCC 
GAGCGCAGCT TGAGCGCCAT CCGCGAGCGC TTCGGGCCCC TGGTGGAGCA GGGCCAATCG 
CGGGCAGCCA CCCTGAGCAC CCTGGCCGGC CAGCCGCTGC TGGAGCGTGC CGAGGCCTGG 
CGCCAGAAGC TGCACGGGCG CCTGGAGGAG GTGGGCGTCC GGGCCCAGGA CCGCCTGGAT 
AAGATACGCC AGCAGCTAGA GGAGGTGCAC GCCAAGGTCG AGGAGCAGGG CAACCAGATG 
CGCCTGCAGG CCGAGGCATT CCAGGCCCGC CTCAGGAGCT GGTTCGAGCC CCTGGTGGAA 
GACATGCAGC GCCAGTGGGC TGGGCTGGTG GAGAAGGTGC AGTTGGCTCT GCGCCCCAGC 
CCCACCTCTC CGCCCAGTGA GAATCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APOE ( XM_005219148.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219205.2 Bos taurus apolipoprotein E (APOE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224776
Clone ID Related Accession (Same CDS sequence)	XM_005219148.4
Accession Version	XM_005219148.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apolipoprotein E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219148.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGATA GCGAAAACCC ACTGTTACTG CCGTTTATTG AGCACCTACT GGGTGCCCAC 
AATCGTCCTA AGATCGGTTG GCCAATCGCA AGCCAGAAGA TGAAGGTTCT GTGGGTTGCC 
GTGGTGGTCG CGCTTCTGGC AGGATGCCAG GCGGATATGG AGGGAGAGCT GGGGCCCGAG 
GAGCCCCTGA CTACCCAGCA GCCCCGGGGG AAGGACAGCC AGCCTTGGGA GCAGGCGCTG 
GGCCGCTTCT GGGATTACCT GCGCTGGGTG CAGACCCTGT CTGACCAGGT GCAGGAGGAG 
CTGCTCAACA CCCAGGTCAT TCAGGAACTG ACGGCGCTGA TGGAGGAGAC CATGAAGGAG 
GTGAAGGCCT ACAAGGAGGA GCTGGAGGGA CAGCTAGGCC CCATGGCCCA GGAGACACAG 
GCCCGCGTGT CCAAGGAGCT GCAGGCAGCC CAGGCCCGGC TAGGCTCCGA CATGGAGGAC 
TTGCGGAACC GCCTGGCACA GTACCGAAGC GAGGTGCAGG CCATGCTGGG CCAGTCCACC 
GAGGAGCTGC GGGCCCGCAT GGCCTCCCAC CTGCGCAAGC TGCGCAAGCG GCTGCTCCGC 
GACGCTGACG ACCTGAAGAA GCGCCTGGCC GTCTACCAAG CTGGGGCCAG CGAGGGTGCC 
GAGCGCAGCT TGAGCGCCAT CCGCGAGCGC TTCGGGCCCC TGGTGGAGCA GGGCCAATCG 
CGGGCAGCCA CCCTGAGCAC CCTGGCCGGC CAGCCGCTGC TGGAGCGTGC CGAGGCCTGG 
CGCCAGAAGC TGCACGGGCG CCTGGAGGAG GTGGGCGTCC GGGCCCAGGA CCGCCTGGAT 
AAGATACGCC AGCAGCTAGA GGAGGTGCAC GCCAAGGTCG AGGAGCAGGG CAACCAGATG 
CGCCTGCAGG CCGAGGCATT CCAGGCCCGC CTCAGGAGCT GGTTCGAGCC CCTGGTGGAA 
GACATGCAGC GCCAGTGGGC TGGGCTGGTG GAGAAGGTGC AGTTGGCTCT GCGCCCCAGC 
CCCACCTCTC CGCCCAGTGA GAATCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219205.2
CDS	274..1323
Translation	MRDSENPLLLPFIEHLLGAHNRPKIGWPIASQKMKVLWVAVVVALLAGCQADMEGELGPEEPLTTQQPRGKDSQPWEQALGRFWDYLRWVQTLSDQVQEELLNTQVIQELTALMEETMKEVKAYKEELEGQLGPMAQETQARVSKELQAAQARLGSDMEDLRNRLAQYRSEVQAMLGQSTEELRARMASHLRKLRKRLLRDADDLKKRLAVYQAGASEGAERSLSAIRERFGPLVEQGQSRAATLSTLAGQPLLERAEAWRQKLHGRLEEVGVRAQDRLDKIRQQLEEVHAKVEEQGNQMRLQAEAFQARLRSWFEPLVEDMQRQWAGLVEKVQLALRPSPTSPPSENH

Target ORF information:

RefSeq Version	XM_005219148.4
Organism	Bos taurus(cattle)
Definition	Bos taurus apolipoprotein E (APOE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219148.4

ORF Insert Sequence:

ATGAGAGATA GCGAAAACCC ACTGTTACTG CCGTTTATTG AGCACCTACT GGGTGCCCAC 
AATCGTCCTA AGATCGGTTG GCCAATCGCA AGCCAGAAGA TGAAGGTTCT GTGGGTTGCC 
GTGGTGGTCG CGCTTCTGGC AGGATGCCAG GCGGATATGG AGGGAGAGCT GGGGCCCGAG 
GAGCCCCTGA CTACCCAGCA GCCCCGGGGG AAGGACAGCC AGCCTTGGGA GCAGGCGCTG 
GGCCGCTTCT GGGATTACCT GCGCTGGGTG CAGACCCTGT CTGACCAGGT GCAGGAGGAG 
CTGCTCAACA CCCAGGTCAT TCAGGAACTG ACGGCGCTGA TGGAGGAGAC CATGAAGGAG 
GTGAAGGCCT ACAAGGAGGA GCTGGAGGGA CAGCTAGGCC CCATGGCCCA GGAGACACAG 
GCCCGCGTGT CCAAGGAGCT GCAGGCAGCC CAGGCCCGGC TAGGCTCCGA CATGGAGGAC 
TTGCGGAACC GCCTGGCACA GTACCGAAGC GAGGTGCAGG CCATGCTGGG CCAGTCCACC 
GAGGAGCTGC GGGCCCGCAT GGCCTCCCAC CTGCGCAAGC TGCGCAAGCG GCTGCTCCGC 
GACGCTGACG ACCTGAAGAA GCGCCTGGCC GTCTACCAAG CTGGGGCCAG CGAGGGTGCC 
GAGCGCAGCT TGAGCGCCAT CCGCGAGCGC TTCGGGCCCC TGGTGGAGCA GGGCCAATCG 
CGGGCAGCCA CCCTGAGCAC CCTGGCCGGC CAGCCGCTGC TGGAGCGTGC CGAGGCCTGG 
CGCCAGAAGC TGCACGGGCG CCTGGAGGAG GTGGGCGTCC GGGCCCAGGA CCGCCTGGAT 
AAGATACGCC AGCAGCTAGA GGAGGTGCAC GCCAAGGTCG AGGAGCAGGG CAACCAGATG 
CGCCTGCAGG CCGAGGCATT CCAGGCCCGC CTCAGGAGCT GGTTCGAGCC CCTGGTGGAA 
GACATGCAGC GCCAGTGGGC TGGGCTGGTG GAGAAGGTGC AGTTGGCTCT GCGCCCCAGC 
CCCACCTCTC CGCCCAGTGA GAATCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APOE ( XM_024977930.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833698.1 Bos taurus apolipoprotein E (APOE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa28801
Clone ID Related Accession (Same CDS sequence)	XM_024977930.1
Accession Version	XM_024977930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apolipoprotein E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTC TGTGGGTTGC CGTGGTGGTC GCGCTTCTGG CAGGATGCCA GGCGGATATG 
GAGGGAGAGC TGGGGCCCGA GGAGCCCCTG ACTACCCAGC AGCCCCGGGG GAAGGACAGC 
CAGCCTTGGG AGCAGGCGCT GGGCCGCTTC TGGGATTACC TGCGCTGGGT GCAGACCCTG 
TCTGACCAGG TGCAGGAGGA GCTGCTCAAC ACCCAGGTCA TTCAGGAACT GACGGCGCTG 
ATGGAGGAGA CCATGAAGGA GGTGAAGGCC TACAAGGAGG AGCTGGAGGG ACAGCTAGGC 
CCCATGGCCC AGGAGACACA GGCCCGCGTG TCCAAGGAGC TGCAGGCAGC CCAGGCCCGG 
CTAGGCTCCG ACATGGAGGA CTTGCGGAAC CGCCTGGCAC AGTACCGAAG CGAGGTGCAG 
GCCATGCTGG GCCAGTCCAC CGAGGAGCTG CGGGCCCGCA TGGCCTCCCA CCTGCGCAAG 
CTGCGCAAGC GGCTGCTCCG CGACGCTGAC GACCTGAAGA AGCGCCTGGC CGTCTACCAA 
GCTGGGGCCA GCGAGGGTGC CGAGCGCAGC TTGAGCGCCA TCCGCGAGCG CTTCGGGCCC 
CTGGTGGAGC AGGGCCAATC GCGGGCAGCC ACCCTGAGCA CCCTGGCCGG CCAGCCGCTG 
CTGGAGCGTG CCGAGGCCTG GCGCCAGAAG CTGCACGGGC GCCTGGAGGA GGTGGGCGTC 
CGGGCCCAGG ACCGCCTGGA TAAGATACGC CAGCAGCTAG AGGAGGTGCA CGCCAAGGTC 
GAGGAGCAGG GCAACCAGAT GCGCCTGCAG GCCGAGGCAT TCCAGGCCCG CCTCAGGAGC 
TGGTTCGAGC CCCTGGTGGA AGACATGCAG CGCCAGTGGG CTGGGCTGGT GGAGAAGGTG 
CAGTTGGCTC TGCGCCCCAG CCCCACCTCT CCGCCCAGTG AGAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833698.1
CDS	206..1156
Translation	MKVLWVAVVVALLAGCQADMEGELGPEEPLTTQQPRGKDSQPWEQALGRFWDYLRWVQTLSDQVQEELLNTQVIQELTALMEETMKEVKAYKEELEGQLGPMAQETQARVSKELQAAQARLGSDMEDLRNRLAQYRSEVQAMLGQSTEELRARMASHLRKLRKRLLRDADDLKKRLAVYQAGASEGAERSLSAIRERFGPLVEQGQSRAATLSTLAGQPLLERAEAWRQKLHGRLEEVGVRAQDRLDKIRQQLEEVHAKVEEQGNQMRLQAEAFQARLRSWFEPLVEDMQRQWAGLVEKVQLALRPSPTSPPSENH

Target ORF information:

RefSeq Version	XM_024977930.1
Organism	Bos taurus(cattle)
Definition	Bos taurus apolipoprotein E (APOE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977930.1

ORF Insert Sequence:

ATGAAGGTTC TGTGGGTTGC CGTGGTGGTC GCGCTTCTGG CAGGATGCCA GGCGGATATG 
GAGGGAGAGC TGGGGCCCGA GGAGCCCCTG ACTACCCAGC AGCCCCGGGG GAAGGACAGC 
CAGCCTTGGG AGCAGGCGCT GGGCCGCTTC TGGGATTACC TGCGCTGGGT GCAGACCCTG 
TCTGACCAGG TGCAGGAGGA GCTGCTCAAC ACCCAGGTCA TTCAGGAACT GACGGCGCTG 
ATGGAGGAGA CCATGAAGGA GGTGAAGGCC TACAAGGAGG AGCTGGAGGG ACAGCTAGGC 
CCCATGGCCC AGGAGACACA GGCCCGCGTG TCCAAGGAGC TGCAGGCAGC CCAGGCCCGG 
CTAGGCTCCG ACATGGAGGA CTTGCGGAAC CGCCTGGCAC AGTACCGAAG CGAGGTGCAG 
GCCATGCTGG GCCAGTCCAC CGAGGAGCTG CGGGCCCGCA TGGCCTCCCA CCTGCGCAAG 
CTGCGCAAGC GGCTGCTCCG CGACGCTGAC GACCTGAAGA AGCGCCTGGC CGTCTACCAA 
GCTGGGGCCA GCGAGGGTGC CGAGCGCAGC TTGAGCGCCA TCCGCGAGCG CTTCGGGCCC 
CTGGTGGAGC AGGGCCAATC GCGGGCAGCC ACCCTGAGCA CCCTGGCCGG CCAGCCGCTG 
CTGGAGCGTG CCGAGGCCTG GCGCCAGAAG CTGCACGGGC GCCTGGAGGA GGTGGGCGTC 
CGGGCCCAGG ACCGCCTGGA TAAGATACGC CAGCAGCTAG AGGAGGTGCA CGCCAAGGTC 
GAGGAGCAGG GCAACCAGAT GCGCCTGCAG GCCGAGGCAT TCCAGGCCCG CCTCAGGAGC 
TGGTTCGAGC CCCTGGTGGA AGACATGCAG CGCCAGTGGG CTGGGCTGGT GGAGAAGGTG 
CAGTTGGCTC TGCGCCCCAG CCCCACCTCT CCGCCCAGTG AGAATCATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro.
Journal of dairy science97(3)1328-35(2014 Mar)
Liu L,Li X,Li Y,Guan Y,Song Y,Yin L,Chen H,Lei L,Liu J,Li X,Wang Z,Yang X,Liu G

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Abundance of mRNA of apolipoprotein b100, apolipoprotein e, and microsomal triglyceride transfer protein in liver from periparturient dairy cows.
Journal of dairy science87(9)2881-8(2004 Sep)
Bernabucci U,Ronchi B,Basiric? L,Pirazzi D,Rueca F,Lacetera N,Nardone A

Distribution of apolipoprotein E among lipoprotein fractions in the lactating cow.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology136(4)905-12(2003 Dec)
Takahashi Y,Itoh F,Oohashi T,Miyamoto T

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APOE cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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