KDM6A cDNA ORF clone, Bos taurus(cattle)

The following KDM6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238237 XM_024987731.1
Latest version!
Bos taurus lysine demethylase 6A (KDM6A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa238238 XM_024987732.1
Latest version!
Bos taurus lysine demethylase 6A (KDM6A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa238239 XM_024987733.1
Latest version!
Bos taurus lysine demethylase 6A (KDM6A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa238240 XM_024987735.1
Latest version!
Bos taurus lysine demethylase 6A (KDM6A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa238241 XM_024987736.1
Latest version!
Bos taurus lysine demethylase 6A (KDM6A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa238242 XM_024987737.1
Latest version!
Bos taurus lysine demethylase 6A (KDM6A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa39942 NM_001206575.1
Latest version!
Bos taurus lysine demethylase 6A (KDM6A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238237
    Clone ID Related Accession (Same CDS sequence) XM_024987731.1
    Accession Version XM_024987731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAG
    ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
    CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT
    TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGGTCA TTCATGGTGT
    TTGACACCAC AGAAATTACA GATGAGACAA ACAGGAGTTA CACAGGTACG ATCTACTGGA
    ATTCCTAACG GGCCATCAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG
    CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTGTCTCAGC CAGGAGTCCG CCCTGCCTGC
    CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA
    AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCACATAAC AGGAAGTGGA
    AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA
    AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG
    CTTCACAAAG GTCAGAGTTC ACATTTGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC
    ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAATGGACAT
    CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC
    ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA
    AACACATCGA TTGTGCCTGA AATAAGCAGG CACGCTGGAG AGACACCTAA CAGCACTGCC
    AGTGTTGAGG GACTGCCTAA TCATGTCCAT CAGGTGACGG CAGATGCTGT TTGCAGTCCT
    AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC
    TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC
    ATCCACCCAG CTGTTCATAC AAAGACTGAT AATTCTGTTG CCTCTTCACC GTCTTCAGCC
    ATTTCCACAG CAACACCTTC TCCAAAATCT ACTGAGCAGA CAACCACAAA CAGTGTTACC
    AGCCTTAATA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAGGAATCT
    CAGAGCCCTA TGAAAACAGA TCTGCTTCTG ATTAGCCACA AACCTAGCCC TCAGATCATA
    CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAC
    CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTATTGG ATAAATGTCC GCCACCAAGA
    CCACCATCTT CACCATACCC TCCCTTGCCA AAGGAAAAGT TGAATCCACC TACACCTAGT
    ATTTATTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT
    CCAAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT GGACCTGGGA
    CTTTTCTCTA CCAAAACTTT GGTAGAAGCT AACAATGAAC ATATGGTGGA AGTGAGGACA
    CAGTTGTTGC AGCCAGCAGA TGAGAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT
    TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCT
    TTCCAGGAAT CATTGAGAGA AGAGAATGAG AAAAGAAGTC ACCATAAAGA CCACTCAGAT
    AATGAATCTA CATCTTCAGA TAATTCTGGG AGGAGAAGGA AAGGACCCTT TAAAACCATA
    AAGTTTGGGA CCAACATTGA CCTGTCTGAT GACAAAAAGT GGAAATTGCA GCTACATGAG
    CTGACTAAAC TTCCTGCTTT TGTGCGTGTT GTATCAGCAG GAAATCTTCT AAGCCACGTT
    GGTCATACCA TACTGGGTAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGAAGCAGA
    ACACCAGGTC ATCAAGAAAA TAACAATTTC TGTTCAGTTA ACATTAATAT TGGTCCAGGT
    GACTGTGAAT GGTTTGTTGT TCCGGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA
    AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ACCTTGAAGA TCTTTATGAA
    GCAAATGTTC CGGTATATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA
    GGCACTGTGC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT
    CCACTTACAG CCTGCCAATA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA
    AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG
    GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTGAGAAC TCTGAAGCAG
    TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCGGGAAAAG AGATTATATG GCATGGGCGA
    ACAAAAGAAG AACCAGCACA TTACTGTAGC ATTTGTGAGG TGGAGGTTTT TAATCTGCTT
    TTTGTCACTA ATGAGAGCAA TTCTCGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA
    AGAAAAACAA GTGGAAACCT GGAAAATTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC
    CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCTC CTCATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843499.1
    CDS356..4615
    Translation

    Target ORF information:

    RefSeq Version XM_024987731.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAG
    ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
    CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT
    TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGGTCA TTCATGGTGT
    TTGACACCAC AGAAATTACA GATGAGACAA ACAGGAGTTA CACAGGTACG ATCTACTGGA
    ATTCCTAACG GGCCATCAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG
    CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTGTCTCAGC CAGGAGTCCG CCCTGCCTGC
    CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA
    AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCACATAAC AGGAAGTGGA
    AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA
    AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG
    CTTCACAAAG GTCAGAGTTC ACATTTGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC
    ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAATGGACAT
    CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC
    ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA
    AACACATCGA TTGTGCCTGA AATAAGCAGG CACGCTGGAG AGACACCTAA CAGCACTGCC
    AGTGTTGAGG GACTGCCTAA TCATGTCCAT CAGGTGACGG CAGATGCTGT TTGCAGTCCT
    AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC
    TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC
    ATCCACCCAG CTGTTCATAC AAAGACTGAT AATTCTGTTG CCTCTTCACC GTCTTCAGCC
    ATTTCCACAG CAACACCTTC TCCAAAATCT ACTGAGCAGA CAACCACAAA CAGTGTTACC
    AGCCTTAATA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAGGAATCT
    CAGAGCCCTA TGAAAACAGA TCTGCTTCTG ATTAGCCACA AACCTAGCCC TCAGATCATA
    CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAC
    CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTATTGG ATAAATGTCC GCCACCAAGA
    CCACCATCTT CACCATACCC TCCCTTGCCA AAGGAAAAGT TGAATCCACC TACACCTAGT
    ATTTATTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT
    CCAAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT GGACCTGGGA
    CTTTTCTCTA CCAAAACTTT GGTAGAAGCT AACAATGAAC ATATGGTGGA AGTGAGGACA
    CAGTTGTTGC AGCCAGCAGA TGAGAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT
    TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCT
    TTCCAGGAAT CATTGAGAGA AGAGAATGAG AAAAGAAGTC ACCATAAAGA CCACTCAGAT
    AATGAATCTA CATCTTCAGA TAATTCTGGG AGGAGAAGGA AAGGACCCTT TAAAACCATA
    AAGTTTGGGA CCAACATTGA CCTGTCTGAT GACAAAAAGT GGAAATTGCA GCTACATGAG
    CTGACTAAAC TTCCTGCTTT TGTGCGTGTT GTATCAGCAG GAAATCTTCT AAGCCACGTT
    GGTCATACCA TACTGGGTAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGAAGCAGA
    ACACCAGGTC ATCAAGAAAA TAACAATTTC TGTTCAGTTA ACATTAATAT TGGTCCAGGT
    GACTGTGAAT GGTTTGTTGT TCCGGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA
    AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ACCTTGAAGA TCTTTATGAA
    GCAAATGTTC CGGTATATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA
    GGCACTGTGC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT
    CCACTTACAG CCTGCCAATA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA
    AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG
    GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTGAGAAC TCTGAAGCAG
    TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCGGGAAAAG AGATTATATG GCATGGGCGA
    ACAAAAGAAG AACCAGCACA TTACTGTAGC ATTTGTGAGG TGGAGGTTTT TAATCTGCTT
    TTTGTCACTA ATGAGAGCAA TTCTCGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA
    AGAAAAACAA GTGGAAACCT GGAAAATTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC
    CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCTC CTCATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238238
    Clone ID Related Accession (Same CDS sequence) XM_024987732.1
    Accession Version XM_024987732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
    AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAGACACC AATTTCACAG
    CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
    AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ATAATTGGAG TAGTGGCCAT
    GCAGTGTCAC ATCCTCCAGT ACAGCAACAA GGTCATTCAT GGTGTTTGAC ACCACAGAAA
    TTACAGCACT TGGAACAGCT CCGTGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG
    ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA
    GGAGTTACAC AGGTACGATC TACTGGAATT CCTAACGGGC CATCAGCTGA CTCATCACTG
    CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTG
    TCTCAGCCAG GAGTCCGCCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT
    GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA
    GGCAATAATC ACATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC
    GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA
    AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTTGGCAGGT
    CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC
    TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG
    GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC
    TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATCGATTG TGCCTGAAAT AAGCAGGCAC
    GCTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TGCCTAATCA TGTCCATCAG
    GTGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA
    AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT
    ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAT
    TCTGTTGCCT CTTCACCGTC TTCAGCCATT TCCACAGCAA CACCTTCTCC AAAATCTACT
    GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAATAGCC CTCACAGTGG GCTACACACA
    ATTAATGGAG AAGGGATGGA GGAATCTCAG AGCCCTATGA AAACAGATCT GCTTCTGATT
    AGCCACAAAC CTAGCCCTCA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA
    GCAGAAGTTC TGAAGGCATG CAGGAACCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT
    TTATTGGATA AATGTCCGCC ACCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG
    GAAAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT
    CCTCCATTAC ATCAATTTTG TACAAATCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT
    GCTGGAGCTC TTAAGTTGGA CCTGGGACTT TTCTCTACCA AAACTTTGGT AGAAGCTAAC
    AATGAACATA TGGTGGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA GAACTGGGAT
    CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT
    AAATATGCAC AGTACCAGGC CTCCTCTTTC CAGGAATCAT TGAGAGAAGA GAATGAGAAA
    AGAAGTCACC ATAAAGACCA CTCAGATAAT GAATCTACAT CTTCAGATAA TTCTGGGAGG
    AGAAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ACATTGACCT GTCTGATGAC
    AAAAAGTGGA AATTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTTGTA
    TCAGCAGGAA ATCTTCTAAG CCACGTTGGT CATACCATAC TGGGTATGAA CACAGTTCAA
    CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAATTTCTGT
    TCAGTTAACA TTAATATTGG TCCAGGTGAC TGTGAATGGT TTGTTGTTCC GGAAGGTTAC
    TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG
    TGGCCCAACC TTGAAGATCT TTATGAAGCA AATGTTCCGG TATATAGGTT TATTCAGCGA
    CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTGCATT GGGTTCAGGC TATTGGCTGG
    TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAATATAA ATTGGCAGTG
    GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT
    TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG
    TATTGTCTTC TGAGAACTCT GAAGCAGTGT CAGACATTGA GGGAAGCTCT CATTGCTGCG
    GGAAAAGAGA TTATATGGCA TGGGCGAACA AAAGAAGAAC CAGCACATTA CTGTAGCATT
    TGTGAGGTGG AGGTTTTTAA TCTGCTTTTT GTCACTAATG AGAGCAATTC TCGAAAGACC
    TACATAGTAC ATTGCCAAGA TTGTGCAAGA AAAACAAGTG GAAACCTGGA AAATTTTGTG
    GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT
    CCTCCATTAC CATCCTCCTC ATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843500.1
    CDS358..4584
    Translation

    Target ORF information:

    RefSeq Version XM_024987732.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT
    AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAGACACC AATTTCACAG
    CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC
    AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ATAATTGGAG TAGTGGCCAT
    GCAGTGTCAC ATCCTCCAGT ACAGCAACAA GGTCATTCAT GGTGTTTGAC ACCACAGAAA
    TTACAGCACT TGGAACAGCT CCGTGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG
    ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA
    GGAGTTACAC AGGTACGATC TACTGGAATT CCTAACGGGC CATCAGCTGA CTCATCACTG
    CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTG
    TCTCAGCCAG GAGTCCGCCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT
    GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA
    GGCAATAATC ACATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC
    GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA
    AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTTGGCAGGT
    CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC
    TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG
    GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC
    TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATCGATTG TGCCTGAAAT AAGCAGGCAC
    GCTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TGCCTAATCA TGTCCATCAG
    GTGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA
    AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT
    ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAT
    TCTGTTGCCT CTTCACCGTC TTCAGCCATT TCCACAGCAA CACCTTCTCC AAAATCTACT
    GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAATAGCC CTCACAGTGG GCTACACACA
    ATTAATGGAG AAGGGATGGA GGAATCTCAG AGCCCTATGA AAACAGATCT GCTTCTGATT
    AGCCACAAAC CTAGCCCTCA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA
    GCAGAAGTTC TGAAGGCATG CAGGAACCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT
    TTATTGGATA AATGTCCGCC ACCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG
    GAAAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT
    CCTCCATTAC ATCAATTTTG TACAAATCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT
    GCTGGAGCTC TTAAGTTGGA CCTGGGACTT TTCTCTACCA AAACTTTGGT AGAAGCTAAC
    AATGAACATA TGGTGGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA GAACTGGGAT
    CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT
    AAATATGCAC AGTACCAGGC CTCCTCTTTC CAGGAATCAT TGAGAGAAGA GAATGAGAAA
    AGAAGTCACC ATAAAGACCA CTCAGATAAT GAATCTACAT CTTCAGATAA TTCTGGGAGG
    AGAAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ACATTGACCT GTCTGATGAC
    AAAAAGTGGA AATTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTTGTA
    TCAGCAGGAA ATCTTCTAAG CCACGTTGGT CATACCATAC TGGGTATGAA CACAGTTCAA
    CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAATTTCTGT
    TCAGTTAACA TTAATATTGG TCCAGGTGAC TGTGAATGGT TTGTTGTTCC GGAAGGTTAC
    TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG
    TGGCCCAACC TTGAAGATCT TTATGAAGCA AATGTTCCGG TATATAGGTT TATTCAGCGA
    CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTGCATT GGGTTCAGGC TATTGGCTGG
    TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAATATAA ATTGGCAGTG
    GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT
    TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG
    TATTGTCTTC TGAGAACTCT GAAGCAGTGT CAGACATTGA GGGAAGCTCT CATTGCTGCG
    GGAAAAGAGA TTATATGGCA TGGGCGAACA AAAGAAGAAC CAGCACATTA CTGTAGCATT
    TGTGAGGTGG AGGTTTTTAA TCTGCTTTTT GTCACTAATG AGAGCAATTC TCGAAAGACC
    TACATAGTAC ATTGCCAAGA TTGTGCAAGA AAAACAAGTG GAAACCTGGA AAATTTTGTG
    GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT
    CCTCCATTAC CATCCTCCTC ATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238239
    Clone ID Related Accession (Same CDS sequence) XM_024987733.1
    Accession Version XM_024987733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA
    CAGCAACAAG GTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC
    CGTGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
    CAGTTTGTCT TAATGCAGCA ACACCAAATG AGACAAACAG GAGTTACACA GGTACGATCT
    ACTGGAATTC CTAACGGGCC ATCAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
    CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTGT CTCAGCCAGG AGTCCGCCCT
    GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
    ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA CATAACAGGA
    AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
    CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
    CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTC
    TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT
    GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
    TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
    TCAGGAAACA CATCGATTGT GCCTGAAATA AGCAGGCACG CTGGAGAGAC ACCTAACAGC
    ACTGCCAGTG TTGAGGGACT GCCTAATCAT GTCCATCAGG TGACGGCAGA TGCTGTTTGC
    AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
    TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
    AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCGTCT
    TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCTACTG AGCAGACAAC CACAAACAGT
    GTTACCAGCC TTAATAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAG
    GAATCTCAGA GCCCTATGAA AACAGATCTG CTTCTGATTA GCCACAAACC TAGCCCTCAG
    ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
    AGGAACCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TATTGGATAA ATGTCCGCCA
    CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG AAAAGTTGAA TCCACCTACA
    CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
    ACAAATCCAA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTGGAC
    CTGGGACTTT TCTCTACCAA AACTTTGGTA GAAGCTAACA ATGAACATAT GGTGGAAGTG
    AGGACACAGT TGTTGCAGCC AGCAGATGAG AACTGGGATC CCACTGGAAC AAAGAAAATC
    TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
    TCCTCTTTCC AGGAATCATT GAGAGAAGAG AATGAGAAAA GAAGTCACCA TAAAGACCAC
    TCAGATAATG AATCTACATC TTCAGATAAT TCTGGGAGGA GAAGGAAAGG ACCCTTTAAA
    ACCATAAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAGTGGAA ATTGCAGCTA
    CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTTGTAT CAGCAGGAAA TCTTCTAAGC
    CACGTTGGTC ATACCATACT GGGTATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGA
    AGCAGAACAC CAGGTCATCA AGAAAATAAC AATTTCTGTT CAGTTAACAT TAATATTGGT
    CCAGGTGACT GTGAATGGTT TGTTGTTCCG GAAGGTTACT GGGGTGTTCT GAATGACTTC
    TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTT
    TATGAAGCAA ATGTTCCGGT ATATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
    AATGCAGGCA CTGTGCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
    GTTGGTCCAC TTACAGCCTG CCAATATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
    TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
    ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACTCTG
    AAGCAGTGTC AGACATTGAG GGAAGCTCTC ATTGCTGCGG GAAAAGAGAT TATATGGCAT
    GGGCGAACAA AAGAAGAACC AGCACATTAC TGTAGCATTT GTGAGGTGGA GGTTTTTAAT
    CTGCTTTTTG TCACTAATGA GAGCAATTCT CGAAAGACCT ACATAGTACA TTGCCAAGAT
    TGTGCAAGAA AAACAAGTGG AAACCTGGAA AATTTTGTGG TGCTAGAACA GTACAAAATG
    GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCTCCTCA
    TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843501.1
    CDS380..4585
    Translation

    Target ORF information:

    RefSeq Version XM_024987733.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA
    CAGCAACAAG GTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC
    CGTGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT
    CAGTTTGTCT TAATGCAGCA ACACCAAATG AGACAAACAG GAGTTACACA GGTACGATCT
    ACTGGAATTC CTAACGGGCC ATCAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC
    CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTGT CTCAGCCAGG AGTCCGCCCT
    GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC
    ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA CATAACAGGA
    AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC
    CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT
    CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTC
    TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT
    GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC
    TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT
    TCAGGAAACA CATCGATTGT GCCTGAAATA AGCAGGCACG CTGGAGAGAC ACCTAACAGC
    ACTGCCAGTG TTGAGGGACT GCCTAATCAT GTCCATCAGG TGACGGCAGA TGCTGTTTGC
    AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC
    TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC
    AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCGTCT
    TCAGCCATTT CCACAGCAAC ACCTTCTCCA AAATCTACTG AGCAGACAAC CACAAACAGT
    GTTACCAGCC TTAATAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAG
    GAATCTCAGA GCCCTATGAA AACAGATCTG CTTCTGATTA GCCACAAACC TAGCCCTCAG
    ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC
    AGGAACCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TATTGGATAA ATGTCCGCCA
    CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG AAAAGTTGAA TCCACCTACA
    CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT
    ACAAATCCAA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTGGAC
    CTGGGACTTT TCTCTACCAA AACTTTGGTA GAAGCTAACA ATGAACATAT GGTGGAAGTG
    AGGACACAGT TGTTGCAGCC AGCAGATGAG AACTGGGATC CCACTGGAAC AAAGAAAATC
    TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC
    TCCTCTTTCC AGGAATCATT GAGAGAAGAG AATGAGAAAA GAAGTCACCA TAAAGACCAC
    TCAGATAATG AATCTACATC TTCAGATAAT TCTGGGAGGA GAAGGAAAGG ACCCTTTAAA
    ACCATAAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAGTGGAA ATTGCAGCTA
    CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTTGTAT CAGCAGGAAA TCTTCTAAGC
    CACGTTGGTC ATACCATACT GGGTATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGA
    AGCAGAACAC CAGGTCATCA AGAAAATAAC AATTTCTGTT CAGTTAACAT TAATATTGGT
    CCAGGTGACT GTGAATGGTT TGTTGTTCCG GAAGGTTACT GGGGTGTTCT GAATGACTTC
    TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTT
    TATGAAGCAA ATGTTCCGGT ATATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA
    AATGCAGGCA CTGTGCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT
    GTTGGTCCAC TTACAGCCTG CCAATATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA
    TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT
    ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACTCTG
    AAGCAGTGTC AGACATTGAG GGAAGCTCTC ATTGCTGCGG GAAAAGAGAT TATATGGCAT
    GGGCGAACAA AAGAAGAACC AGCACATTAC TGTAGCATTT GTGAGGTGGA GGTTTTTAAT
    CTGCTTTTTG TCACTAATGA GAGCAATTCT CGAAAGACCT ACATAGTACA TTGCCAAGAT
    TGTGCAAGAA AAACAAGTGG AAACCTGGAA AATTTTGTGG TGCTAGAACA GTACAAAATG
    GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCTCCTCA
    TCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238240
    Clone ID Related Accession (Same CDS sequence) XM_024987735.1
    Accession Version XM_024987735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA
    CAGCAACAAG GTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACAAACAGGA
    GTTACACAGG TACGATCTAC TGGAATTCCT AACGGGCCAT CAGCTGACTC ATCACTGCCT
    ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTGTCT
    CAGCCAGGAG TCCGCCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
    GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
    AATAATCACA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
    CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
    CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTT GGCAGGTCCT
    AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
    GGTATTCAGA ATCAGAATGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
    GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
    ACCAAAGAGA GCAAGCCTTC AGGAAACACA TCGATTGTGC CTGAAATAAG CAGGCACGCT
    GGAGAGACAC CTAACAGCAC TGCCAGTGTT GAGGGACTGC CTAATCATGT CCATCAGGTG
    ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
    TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
    GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAATTCT
    GTTGCCTCTT CACCGTCTTC AGCCATTTCC ACAGCAACAC CTTCTCCAAA ATCTACTGAG
    CAGACAACCA CAAACAGTGT TACCAGCCTT AATAGCCCTC ACAGTGGGCT ACACACAATT
    AATGGAGAAG GGATGGAGGA ATCTCAGAGC CCTATGAAAA CAGATCTGCT TCTGATTAGC
    CACAAACCTA GCCCTCAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
    GAAGTTCTGA AGGCATGCAG GAACCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTA
    TTGGATAAAT GTCCGCCACC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAA
    AAGTTGAATC CACCTACACC TAGTATTTAT TTGGAAAATA AACGTGATGC TTTCTTTCCT
    CCATTACATC AATTTTGTAC AAATCCAAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
    GGAGCTCTTA AGTTGGACCT GGGACTTTTC TCTACCAAAA CTTTGGTAGA AGCTAACAAT
    GAACATATGG TGGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAGAA CTGGGATCCC
    ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
    TATGCACAGT ACCAGGCCTC CTCTTTCCAG GAATCATTGA GAGAAGAGAA TGAGAAAAGA
    AGTCACCATA AAGACCACTC AGATAATGAA TCTACATCTT CAGATAATTC TGGGAGGAGA
    AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAACA TTGACCTGTC TGATGACAAA
    AAGTGGAAAT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTTGTATCA
    GCAGGAAATC TTCTAAGCCA CGTTGGTCAT ACCATACTGG GTATGAACAC AGTTCAACTA
    TACATGAAAG TTCCAGGAAG CAGAACACCA GGTCATCAAG AAAATAACAA TTTCTGTTCA
    GTTAACATTA ATATTGGTCC AGGTGACTGT GAATGGTTTG TTGTTCCGGA AGGTTACTGG
    GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
    CCCAACCTTG AAGATCTTTA TGAAGCAAAT GTTCCGGTAT ATAGGTTTAT TCAGCGACCT
    GGAGATTTGG TCTGGATAAA TGCAGGCACT GTGCATTGGG TTCAGGCTAT TGGCTGGTGC
    AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AATATAAATT GGCAGTGGAA
    CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
    TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT
    TGTCTTCTGA GAACTCTGAA GCAGTGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCGGGA
    AAAGAGATTA TATGGCATGG GCGAACAAAA GAAGAACCAG CACATTACTG TAGCATTTGT
    GAGGTGGAGG TTTTTAATCT GCTTTTTGTC ACTAATGAGA GCAATTCTCG AAAGACCTAC
    ATAGTACATT GCCAAGATTG TGCAAGAAAA ACAAGTGGAA ACCTGGAAAA TTTTGTGGTG
    CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT
    CCATTACCAT CCTCCTCATC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843503.1
    CDS364..4467
    Translation

    Target ORF information:

    RefSeq Version XM_024987735.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6A (KDM6A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGA ATACTTCTGA TAATTGGAGT AGTGGCCATG CAGTGTCACA TCCTCCAGTA
    CAGCAACAAG GTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACAAACAGGA
    GTTACACAGG TACGATCTAC TGGAATTCCT AACGGGCCAT CAGCTGACTC ATCACTGCCT
    ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTGTCT
    CAGCCAGGAG TCCGCCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA
    GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC
    AATAATCACA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA
    CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC
    CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTT GGCAGGTCCT
    AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT
    GGTATTCAGA ATCAGAATGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT
    GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA
    ACCAAAGAGA GCAAGCCTTC AGGAAACACA TCGATTGTGC CTGAAATAAG CAGGCACGCT
    GGAGAGACAC CTAACAGCAC TGCCAGTGTT GAGGGACTGC CTAATCATGT CCATCAGGTG
    ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT
    TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT
    GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAATTCT
    GTTGCCTCTT CACCGTCTTC AGCCATTTCC ACAGCAACAC CTTCTCCAAA ATCTACTGAG
    CAGACAACCA CAAACAGTGT TACCAGCCTT AATAGCCCTC ACAGTGGGCT ACACACAATT
    AATGGAGAAG GGATGGAGGA ATCTCAGAGC CCTATGAAAA CAGATCTGCT TCTGATTAGC
    CACAAACCTA GCCCTCAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA
    GAAGTTCTGA AGGCATGCAG GAACCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTA
    TTGGATAAAT GTCCGCCACC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAA
    AAGTTGAATC CACCTACACC TAGTATTTAT TTGGAAAATA AACGTGATGC TTTCTTTCCT
    CCATTACATC AATTTTGTAC AAATCCAAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT
    GGAGCTCTTA AGTTGGACCT GGGACTTTTC TCTACCAAAA CTTTGGTAGA AGCTAACAAT
    GAACATATGG TGGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAGAA CTGGGATCCC
    ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA
    TATGCACAGT ACCAGGCCTC CTCTTTCCAG GAATCATTGA GAGAAGAGAA TGAGAAAAGA
    AGTCACCATA AAGACCACTC AGATAATGAA TCTACATCTT CAGATAATTC TGGGAGGAGA
    AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAACA TTGACCTGTC TGATGACAAA
    AAGTGGAAAT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTTGTATCA
    GCAGGAAATC TTCTAAGCCA CGTTGGTCAT ACCATACTGG GTATGAACAC AGTTCAACTA
    TACATGAAAG TTCCAGGAAG CAGAACACCA GGTCATCAAG AAAATAACAA TTTCTGTTCA
    GTTAACATTA ATATTGGTCC AGGTGACTGT GAATGGTTTG TTGTTCCGGA AGGTTACTGG
    GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG
    CCCAACCTTG AAGATCTTTA TGAAGCAAAT GTTCCGGTAT ATAGGTTTAT TCAGCGACCT
    GGAGATTTGG TCTGGATAAA TGCAGGCACT GTGCATTGGG TTCAGGCTAT TGGCTGGTGC
    AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AATATAAATT GGCAGTGGAA
    CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC
    TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT
    TGTCTTCTGA GAACTCTGAA GCAGTGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCGGGA
    AAAGAGATTA TATGGCATGG GCGAACAAAA GAAGAACCAG CACATTACTG TAGCATTTGT
    GAGGTGGAGG TTTTTAATCT GCTTTTTGTC ACTAATGAGA GCAATTCTCG AAAGACCTAC
    ATAGTACATT GCCAAGATTG TGCAAGAAAA ACAAGTGGAA ACCTGGAAAA TTTTGTGGTG
    CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT
    CCATTACCAT CCTCCTCATC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238241
    Clone ID Related Accession (Same CDS sequence) XM_024987736.1
    Accession Version XM_024987736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
    TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGGTCAT
    TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGTGC AAATAGAAAT
    AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG
    CAGCAACACC AAATGAGACA AACAGGAGTT ACACAGGTAC GATCTACTGG AATTCCTAAC
    GGGCCATCAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT
    GCTCTGACCA GAGTGCCTAG CGTGTCTCAG CCAGGAGTCC GCCCTGCCTG CCCTGGGCAG
    CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG
    GGAAGTACAG ACACTATTTT GATAGGCAAT AATCACATAA CAGGAAGTGG AAGTAATGGA
    AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC
    AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA
    GGTCAGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT
    TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG
    CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC
    TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATCG
    ATTGTGCCTG AAATAAGCAG GCACGCTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG
    GGACTGCCTA ATCATGTCCA TCAGGTGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA
    GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG
    GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA
    GCTGTTCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CGTCTTCAGC CATTTCCACA
    GCAACACCTT CTCCAAAATC TACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAT
    AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAGGAATC TCAGAGCCCT
    ATGAAAACAG ATCTGCTTCT GATTAGCCAC AAACCTAGCC CTCAGATCAT ACCATCAATG
    TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA CCTAGGTAAA
    AATGGCTTAT CTAACAGTAG CATTTTATTG GATAAATGTC CGCCACCAAG ACCACCATCT
    TCACCATACC CTCCCTTGCC AAAGGAAAAG TTGAATCCAC CTACACCTAG TATTTATTTG
    GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCAAACAAC
    CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TGGACCTGGG ACTTTTCTCT
    ACCAAAACTT TGGTAGAAGC TAACAATGAA CATATGGTGG AAGTGAGGAC ACAGTTGTTG
    CAGCCAGCAG ATGAGAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT
    AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC TTTCCAGGAA
    TCATTGAGAG AAGAGAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA TAATGAATCT
    ACATCTTCAG ATAATTCTGG GAGGAGAAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG
    ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAATTGC AGCTACATGA GCTGACTAAA
    CTTCCTGCTT TTGTGCGTGT TGTATCAGCA GGAAATCTTC TAAGCCACGT TGGTCATACC
    ATACTGGGTA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT
    CATCAAGAAA ATAACAATTT CTGTTCAGTT AACATTAATA TTGGTCCAGG TGACTGTGAA
    TGGTTTGTTG TTCCGGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT
    TTGAATTTCC TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTTTATGA AGCAAATGTT
    CCGGTATATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTG
    CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA
    GCCTGCCAAT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG
    TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT
    CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CTCTGAAGCA GTGTCAGACA
    TTGAGGGAAG CTCTCATTGC TGCGGGAAAA GAGATTATAT GGCATGGGCG AACAAAAGAA
    GAACCAGCAC ATTACTGTAG CATTTGTGAG GTGGAGGTTT TTAATCTGCT TTTTGTCACT
    AATGAGAGCA ATTCTCGAAA GACCTACATA GTACATTGCC AAGATTGTGC AAGAAAAACA
    AGTGGAAACC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA
    GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCT CCTCATCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843504.1
    CDS354..4424
    Translation

    Target ORF information:

    RefSeq Version XM_024987736.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6A (KDM6A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
    TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGGTCAT
    TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGTGC AAATAGAAAT
    AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG
    CAGCAACACC AAATGAGACA AACAGGAGTT ACACAGGTAC GATCTACTGG AATTCCTAAC
    GGGCCATCAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT
    GCTCTGACCA GAGTGCCTAG CGTGTCTCAG CCAGGAGTCC GCCCTGCCTG CCCTGGGCAG
    CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG
    GGAAGTACAG ACACTATTTT GATAGGCAAT AATCACATAA CAGGAAGTGG AAGTAATGGA
    AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC
    AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA
    GGTCAGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT
    TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG
    CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC
    TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATCG
    ATTGTGCCTG AAATAAGCAG GCACGCTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG
    GGACTGCCTA ATCATGTCCA TCAGGTGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA
    GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG
    GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA
    GCTGTTCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CGTCTTCAGC CATTTCCACA
    GCAACACCTT CTCCAAAATC TACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAT
    AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAGGAATC TCAGAGCCCT
    ATGAAAACAG ATCTGCTTCT GATTAGCCAC AAACCTAGCC CTCAGATCAT ACCATCAATG
    TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA CCTAGGTAAA
    AATGGCTTAT CTAACAGTAG CATTTTATTG GATAAATGTC CGCCACCAAG ACCACCATCT
    TCACCATACC CTCCCTTGCC AAAGGAAAAG TTGAATCCAC CTACACCTAG TATTTATTTG
    GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCAAACAAC
    CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TGGACCTGGG ACTTTTCTCT
    ACCAAAACTT TGGTAGAAGC TAACAATGAA CATATGGTGG AAGTGAGGAC ACAGTTGTTG
    CAGCCAGCAG ATGAGAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT
    AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC TTTCCAGGAA
    TCATTGAGAG AAGAGAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA TAATGAATCT
    ACATCTTCAG ATAATTCTGG GAGGAGAAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG
    ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAATTGC AGCTACATGA GCTGACTAAA
    CTTCCTGCTT TTGTGCGTGT TGTATCAGCA GGAAATCTTC TAAGCCACGT TGGTCATACC
    ATACTGGGTA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT
    CATCAAGAAA ATAACAATTT CTGTTCAGTT AACATTAATA TTGGTCCAGG TGACTGTGAA
    TGGTTTGTTG TTCCGGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT
    TTGAATTTCC TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTTTATGA AGCAAATGTT
    CCGGTATATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTG
    CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA
    GCCTGCCAAT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG
    TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT
    CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CTCTGAAGCA GTGTCAGACA
    TTGAGGGAAG CTCTCATTGC TGCGGGAAAA GAGATTATAT GGCATGGGCG AACAAAAGAA
    GAACCAGCAC ATTACTGTAG CATTTGTGAG GTGGAGGTTT TTAATCTGCT TTTTGTCACT
    AATGAGAGCA ATTCTCGAAA GACCTACATA GTACATTGCC AAGATTGTGC AAGAAAAACA
    AGTGGAAACC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA
    GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCT CCTCATCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238242
    Clone ID Related Accession (Same CDS sequence) XM_024987737.1
    Accession Version XM_024987737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
    TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGGTCAT
    TCATGGTGTT TGACACCACA GAAATTACAG ATGAGACAAA CAGGAGTTAC ACAGGTACGA
    TCTACTGGAA TTCCTAACGG GCCATCAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT
    GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TGTCTCAGCC AGGAGTCCGC
    CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT
    AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCACATAACA
    GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT
    AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC
    ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTTGGCAG GTCCTAATGG TGAACGACCT
    CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG
    AATGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC
    TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG
    CCTTCAGGAA ACACATCGAT TGTGCCTGAA ATAAGCAGGC ACGCTGGAGA GACACCTAAC
    AGCACTGCCA GTGTTGAGGG ACTGCCTAAT CATGTCCATC AGGTGACGGC AGATGCTGTT
    TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG
    CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA
    GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ATTCTGTTGC CTCTTCACCG
    TCTTCAGCCA TTTCCACAGC AACACCTTCT CCAAAATCTA CTGAGCAGAC AACCACAAAC
    AGTGTTACCA GCCTTAATAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG
    GAGGAATCTC AGAGCCCTAT GAAAACAGAT CTGCTTCTGA TTAGCCACAA ACCTAGCCCT
    CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA
    TGCAGGAACC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTATTGGA TAAATGTCCG
    CCACCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGAAAAGTT GAATCCACCT
    ACACCTAGTA TTTATTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT
    TGTACAAATC CAAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTG
    GACCTGGGAC TTTTCTCTAC CAAAACTTTG GTAGAAGCTA ACAATGAACA TATGGTGGAA
    GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAGAACTGGG ATCCCACTGG AACAAAGAAA
    ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG
    GCCTCCTCTT TCCAGGAATC ATTGAGAGAA GAGAATGAGA AAAGAAGTCA CCATAAAGAC
    CACTCAGATA ATGAATCTAC ATCTTCAGAT AATTCTGGGA GGAGAAGGAA AGGACCCTTT
    AAAACCATAA AGTTTGGGAC CAACATTGAC CTGTCTGATG ACAAAAAGTG GAAATTGCAG
    CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTTG TATCAGCAGG AAATCTTCTA
    AGCCACGTTG GTCATACCAT ACTGGGTATG AACACAGTTC AACTATACAT GAAAGTTCCA
    GGAAGCAGAA CACCAGGTCA TCAAGAAAAT AACAATTTCT GTTCAGTTAA CATTAATATT
    GGTCCAGGTG ACTGTGAATG GTTTGTTGTT CCGGAAGGTT ACTGGGGTGT TCTGAATGAC
    TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA CCTTGAAGAT
    CTTTATGAAG CAAATGTTCC GGTATATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG
    ATAAATGCAG GCACTGTGCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG
    AATGTTGGTC CACTTACAGC CTGCCAATAT AAATTGGCAG TGGAACGGTA CGAATGGAAC
    AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA
    AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTGAGAACT
    CTGAAGCAGT GTCAGACATT GAGGGAAGCT CTCATTGCTG CGGGAAAAGA GATTATATGG
    CATGGGCGAA CAAAAGAAGA ACCAGCACAT TACTGTAGCA TTTGTGAGGT GGAGGTTTTT
    AATCTGCTTT TTGTCACTAA TGAGAGCAAT TCTCGAAAGA CCTACATAGT ACATTGCCAA
    GATTGTGCAA GAAAAACAAG TGGAAACCTG GAAAATTTTG TGGTGCTAGA ACAGTACAAA
    ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCTCC
    TCATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843505.1
    CDS355..4323
    Translation

    Target ORF information:

    RefSeq Version XM_024987737.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6A (KDM6A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT
    TCTGATAATT GGAGTAGTGG CCATGCAGTG TCACATCCTC CAGTACAGCA ACAAGGTCAT
    TCATGGTGTT TGACACCACA GAAATTACAG ATGAGACAAA CAGGAGTTAC ACAGGTACGA
    TCTACTGGAA TTCCTAACGG GCCATCAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT
    GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TGTCTCAGCC AGGAGTCCGC
    CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT
    AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCACATAACA
    GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT
    AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC
    ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTTGGCAG GTCCTAATGG TGAACGACCT
    CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG
    AATGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC
    TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG
    CCTTCAGGAA ACACATCGAT TGTGCCTGAA ATAAGCAGGC ACGCTGGAGA GACACCTAAC
    AGCACTGCCA GTGTTGAGGG ACTGCCTAAT CATGTCCATC AGGTGACGGC AGATGCTGTT
    TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG
    CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA
    GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ATTCTGTTGC CTCTTCACCG
    TCTTCAGCCA TTTCCACAGC AACACCTTCT CCAAAATCTA CTGAGCAGAC AACCACAAAC
    AGTGTTACCA GCCTTAATAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG
    GAGGAATCTC AGAGCCCTAT GAAAACAGAT CTGCTTCTGA TTAGCCACAA ACCTAGCCCT
    CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA
    TGCAGGAACC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTATTGGA TAAATGTCCG
    CCACCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGAAAAGTT GAATCCACCT
    ACACCTAGTA TTTATTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT
    TGTACAAATC CAAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTG
    GACCTGGGAC TTTTCTCTAC CAAAACTTTG GTAGAAGCTA ACAATGAACA TATGGTGGAA
    GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAGAACTGGG ATCCCACTGG AACAAAGAAA
    ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG
    GCCTCCTCTT TCCAGGAATC ATTGAGAGAA GAGAATGAGA AAAGAAGTCA CCATAAAGAC
    CACTCAGATA ATGAATCTAC ATCTTCAGAT AATTCTGGGA GGAGAAGGAA AGGACCCTTT
    AAAACCATAA AGTTTGGGAC CAACATTGAC CTGTCTGATG ACAAAAAGTG GAAATTGCAG
    CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTTG TATCAGCAGG AAATCTTCTA
    AGCCACGTTG GTCATACCAT ACTGGGTATG AACACAGTTC AACTATACAT GAAAGTTCCA
    GGAAGCAGAA CACCAGGTCA TCAAGAAAAT AACAATTTCT GTTCAGTTAA CATTAATATT
    GGTCCAGGTG ACTGTGAATG GTTTGTTGTT CCGGAAGGTT ACTGGGGTGT TCTGAATGAC
    TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA CCTTGAAGAT
    CTTTATGAAG CAAATGTTCC GGTATATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG
    ATAAATGCAG GCACTGTGCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG
    AATGTTGGTC CACTTACAGC CTGCCAATAT AAATTGGCAG TGGAACGGTA CGAATGGAAC
    AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA
    AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTGAGAACT
    CTGAAGCAGT GTCAGACATT GAGGGAAGCT CTCATTGCTG CGGGAAAAGA GATTATATGG
    CATGGGCGAA CAAAAGAAGA ACCAGCACAT TACTGTAGCA TTTGTGAGGT GGAGGTTTTT
    AATCTGCTTT TTGTCACTAA TGAGAGCAAT TCTCGAAAGA CCTACATAGT ACATTGCCAA
    GATTGTGCAA GAAAAACAAG TGGAAACCTG GAAAATTTTG TGGTGCTAGA ACAGTACAAA
    ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCTCC
    TCATCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39942
    Clone ID Related Accession (Same CDS sequence) NM_001206575.1
    Accession Version NM_001206575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-27
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6A
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000030.1. On or before May 4, 2011 this sequence version replaced XM_002707354.1, XM_002707355.1, XM_580363.5, XM_002707356.1, XM_002700179.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721694.1953.1, SRR6764281.5574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAG
    ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
    CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT
    TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGGTCA TTCATGGTGT
    TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGTG CAAATAGAAA TAATTTAAAT
    CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC
    CAAATGAGAC AAACAGGAGT TACACAGGTA CGATCTACTG GAATTCCTAA CGGGCCATCA
    GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
    AGAGTGCCTA GCGTGTCTCA GCCAGGAGTC CGCCCTGCCT GCCCTGGGCA GCCTTTGGCC
    AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
    GACACTATTT TGATAGGCAA TAATCACATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
    TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
    GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
    TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
    CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT
    TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
    CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATC GATTGTGCCT
    GAAATAAGCA GGCACGCTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTGCCT
    AATCATGTCC ATCAGGTGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
    TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
    AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
    ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCGTCTTCAG CCATTTCCAC AGCAACACCT
    TCTCCAAAAT CTACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA TAGCCCTCAC
    AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAGGAAT CTCAGAGCCC TATGAAAACA
    GATCTGCTTC TGATTAGCCA CAAACCTAGC CCTCAGATCA TACCATCAAT GTCTGTGTCC
    ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ACCTAGGTAA AAATGGCTTA
    TCTAACAGTA GCATTTTATT GGATAAATGT CCGCCACCAA GACCACCATC TTCACCATAC
    CCTCCCTTGC CAAAGGAAAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA
    CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCAAACAA CCCTGTTACA
    GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTGGACCTGG GACTTTTCTC TACCAAAACT
    TTGGTAGAAG CTAACAATGA ACATATGGTG GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
    GATGAGAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
    CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CTTTCCAGGA ATCATTGAGA
    GAAGAGAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ATAATGAATC TACATCTTCA
    GATAATTCTG GGAGGAGAAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAACATT
    GACCTGTCTG ATGACAAAAA GTGGAAATTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
    TTTGTGCGTG TTGTATCAGC AGGAAATCTT CTAAGCCACG TTGGTCATAC CATACTGGGT
    ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA
    AATAACAATT TCTGTTCAGT TAACATTAAT ATTGGTCCAG GTGACTGTGA ATGGTTTGTT
    GTTCCGGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
    CTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTTTATG AAGCAAATGT TCCGGTATAT
    AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT GCATTGGGTT
    CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAA
    TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
    CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
    TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACTCTGAAGC AGTGTCAGAC ATTGAGGGAA
    GCTCTCATTG CTGCGGGAAA AGAGATTATA TGGCATGGGC GAACAAAAGA AGAACCAGCA
    CATTACTGTA GCATTTGTGA GGTGGAGGTT TTTAATCTGC TTTTTGTCAC TAATGAGAGC
    AATTCTCGAA AGACCTACAT AGTACATTGC CAAGATTGTG CAAGAAAAAC AAGTGGAAAC
    CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC
    CAATTTACAT TAGCTCCTCC ATTACCATCC TCCTCATCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193504.1
    CDS18..4379
    Translation

    Target ORF information:

    RefSeq Version NM_001206575.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6A (KDM6A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAATCCA GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
    GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGGGAGA GCGAGGAGGC GTCCCCGAGC
    CTGACAGCCG AGGAGAGAGA GGCGCTCGGC GGACTGGATA GCCGCCTCTT CGGGTTCGTA
    AGGTTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCCGT TCGCTGCTAC
    GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT
    CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC
    AGTTTACAAT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC
    TTCCATTACA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT
    CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGGCTTG GGCTTATGTT CAAAGTGAAC
    ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC
    ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAG
    TATCATTCTG CAAAGGAAGC TTATGAACAA CTTTTGCAGA CAGAAAACCT TTCTGCACAA
    GTAAAAGCAA CTGTCTTACA ACAGTTGGGA TGGATGCATC ATACTGTGGA TCTCCTGGGA
    GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT
    CCTAATTCTG GTCAGTCCTG GTATTTCCTT GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT
    CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAGT CAGAAGCAAG TGCAGATACA
    TGGTGTTCAA TAGGCGTGCT ATATCAACAG CAAAATCAGC CTATGGATGC TTTACAGGCC
    TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACA
    CTCTACGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACCAGA
    AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG
    TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG
    GAGGCGTGGA GCCTACCAAT CCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA
    GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAG
    ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC
    CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGATAAT
    TGGAGTAGTG GCCATGCAGT GTCACATCCT CCAGTACAGC AACAAGGTCA TTCATGGTGT
    TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGTG CAAATAGAAA TAATTTAAAT
    CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC
    CAAATGAGAC AAACAGGAGT TACACAGGTA CGATCTACTG GAATTCCTAA CGGGCCATCA
    GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC
    AGAGTGCCTA GCGTGTCTCA GCCAGGAGTC CGCCCTGCCT GCCCTGGGCA GCCTTTGGCC
    AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA
    GACACTATTT TGATAGGCAA TAATCACATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT
    TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA
    GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT
    TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT
    CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT
    TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA
    CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATC GATTGTGCCT
    GAAATAAGCA GGCACGCTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTGCCT
    AATCATGTCC ATCAGGTGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG
    TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC
    AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT
    ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCGTCTTCAG CCATTTCCAC AGCAACACCT
    TCTCCAAAAT CTACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA TAGCCCTCAC
    AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAGGAAT CTCAGAGCCC TATGAAAACA
    GATCTGCTTC TGATTAGCCA CAAACCTAGC CCTCAGATCA TACCATCAAT GTCTGTGTCC
    ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ACCTAGGTAA AAATGGCTTA
    TCTAACAGTA GCATTTTATT GGATAAATGT CCGCCACCAA GACCACCATC TTCACCATAC
    CCTCCCTTGC CAAAGGAAAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA
    CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCAAACAA CCCTGTTACA
    GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTGGACCTGG GACTTTTCTC TACCAAAACT
    TTGGTAGAAG CTAACAATGA ACATATGGTG GAAGTGAGGA CACAGTTGTT GCAGCCAGCA
    GATGAGAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT
    CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CTTTCCAGGA ATCATTGAGA
    GAAGAGAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ATAATGAATC TACATCTTCA
    GATAATTCTG GGAGGAGAAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAACATT
    GACCTGTCTG ATGACAAAAA GTGGAAATTG CAGCTACATG AGCTGACTAA ACTTCCTGCT
    TTTGTGCGTG TTGTATCAGC AGGAAATCTT CTAAGCCACG TTGGTCATAC CATACTGGGT
    ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA
    AATAACAATT TCTGTTCAGT TAACATTAAT ATTGGTCCAG GTGACTGTGA ATGGTTTGTT
    GTTCCGGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC
    CTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTTTATG AAGCAAATGT TCCGGTATAT
    AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT GCATTGGGTT
    CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAA
    TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA
    CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT
    TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACTCTGAAGC AGTGTCAGAC ATTGAGGGAA
    GCTCTCATTG CTGCGGGAAA AGAGATTATA TGGCATGGGC GAACAAAAGA AGAACCAGCA
    CATTACTGTA GCATTTGTGA GGTGGAGGTT TTTAATCTGC TTTTTGTCAC TAATGAGAGC
    AATTCTCGAA AGACCTACAT AGTACATTGC CAAGATTGTG CAAGAAAAAC AAGTGGAAAC
    CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC
    CAATTTACAT TAGCTCCTCC ATTACCATCC TCCTCATCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.