NF1 cDNA ORF clone, Bos taurus(cattle)

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa41114 NM_001122728.1
Latest version!
Bos taurus neurofibromin 1 (NF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa261932 NM_001122728.2
Latest version!
Bos taurus neurofibromin 1 (NF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa261933 NM_001372007.1
Latest version!
Bos taurus neurofibromin 1 (NF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa41114
    Clone ID Related Accession (Same CDS sequence) NM_001122728.1
    Accession Version NM_001122728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product neurofibromin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU442576.1. ##Evidence-Data-START## Transcript exon combination :: EU442576.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACTGA GCACAACAAG
    GAATGTCTTA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGTGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGGTGAGT ATTTGGAGAA GCTGCTGGAA AAAATTTGTA
    TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT CTTGCTGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCATACTT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTGTTATTTT CTCTTAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAC AATGTGGATG TTCATGATAT AGAACTGTTA
    CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAAGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAGC TGTACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCTGA AAAACTGTTT GACTTAGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTGC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCTAAGGA TGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTCAC TGAAAGTGCT
    GCAATCGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATCTTTC TGCTGGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT GTTTAATCCG
    AGTAAGCCAT TCTCGAGGGG CAGTCAGCCG GCAGATGTGG ACCTAATGAT CGACTGCCTT
    GTCTCTTGCT TCCGGATAAG CCCTCACAAT AACCAACACT TCAAGATCTG CCTGGCTCAG
    AATTCCCCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GCCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTCCAGGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTACAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CGAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACTGCAG AATTAATTAC AGGGCTTGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCCCAGG AAGCGATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAAATTA GGTATATGCA CCCTTATTTT
    TTAATTCAAC TTAAATTTTT AATTTTTTTT GTCTTTAAAT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001116200.1
    CDS1..1785
    Translation

    Target ORF information:

    RefSeq Version NM_001122728.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofibromin 1 (NF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001122728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACTGA GCACAACAAG
    GAATGTCTTA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGTGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGGTGAGT ATTTGGAGAA GCTGCTGGAA AAAATTTGTA
    TCTCTCTCAG TTGATTATAT TGGATACACT GGAAAAATGT CTTGCTGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCATACTT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTGTTATTTT CTCTTAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAC AATGTGGATG TTCATGATAT AGAACTGTTA
    CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAAGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAGC TGTACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCTGA AAAACTGTTT GACTTAGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTGC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCTAAGGA TGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTCAC TGAAAGTGCT
    GCAATCGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATCTTTC TGCTGGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT GTTTAATCCG
    AGTAAGCCAT TCTCGAGGGG CAGTCAGCCG GCAGATGTGG ACCTAATGAT CGACTGCCTT
    GTCTCTTGCT TCCGGATAAG CCCTCACAAT AACCAACACT TCAAGATCTG CCTGGCTCAG
    AATTCCCCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GCCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTCCAGGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTACAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CGAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACTGCAG AATTAATTAC AGGGCTTGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCCCAGG AAGCGATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAAATTA GGTATATGCA CCCTTATTTT
    TTAATTCAAC TTAAATTTTT AATTTTTTTT GTCTTTAAAT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa261932
    Clone ID Related Accession (Same CDS sequence) NM_001122728.2
    Accession Version NM_001122728.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-30
    Organism Bos taurus(cattle)
    Product neurofibromin isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000019.1. On Jul 31, 2019 this sequence version replaced NM_001122728.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACTGA GCACAACAAG
    GAATGTCTTA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGTGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCATACTT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTGTTATTTT CTCTTAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAC AATGTGGATG TTCATGATAT AGAACTGTTA
    CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAAGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAGC TGTACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCTGA AAAACTGTTT GACTTAGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTGC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCTAAGGA TGTGGTTGAT GAAAACAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GAAAAGCACT TGCCGGCCAT GGAGGAAGCA GGCAGCTCAC TGAAAGTGCT
    GCAATCGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATCTTTC TGCTGGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT GTTTAATCCG
    AGTAAGCCAT TCTCGAGGGG CAGTCAGCCG GCAGATGTGG ACCTAATGAT CGACTGCCTT
    GTCTCTTGCT TCCGGATAAG CCCTCACAAT AACCAACACT TCAAGATCTG CCTGGCTCAG
    AATTCCCCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GCCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTCCAGGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTACAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CGAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACTGCAG AATTAATTAC AGGGCTTGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCCCAGG AAGCGATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATTTGCAAGA AATTGACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCTTCCT TTATGGAGTA GGATGTGATG TTCCTTCAAG TGGGAATACC
    AGTCAAATGT CCATGGATCA TGACGAATTA CTACGCACTT GTGCTCCTGG AACCTCTCTC
    AGGAAGGGGA AAGGAAATTC ATCCATGGAT AGTGCAGCAG GGTGCAGTGG AACCCCACCA
    ATATGCCGAC AAGCCCAGAC CAAACTAGAA GTGGCCCTGT ACATGTTCCT GTGGAGCCCC
    GACACTGAAG CAGTTCTGGT TGCCATGTCC TGTTTTCGTC ACCTCTGTGA GGAAGCAGAT
    ATCCGGTGTG GGGTGGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CTTCTGTTAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCGC TACTACGGCG AATTGAGCAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACGCATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CACAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTA TCTGACACGG ACTCACTACA AGAATGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCTTAGCAA CATATAGCCC ACCCATGGGC CCTGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CTTCAGAGGG AAATGCAGAT ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTGTCCTTAA TGGTCTGTAA CCATGAGAAA GTGGGACTTC AAATACGGAC CAATGTTAAG
    GATCTGGTGG GTCTAGAATT GAGTCCTGCT CTTTATCCAA TGCTTTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAGTTTGTGG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACCGAAGGC
    AGTTCTGAAC ATCTGGGGCA AGCCAGCATT GAAACAATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGAAATAT GGTTCATGCA ATTCAAATAA AAACAAAACT GTGTCAGTTA
    GTCGAGGTAA TGATGGCAAG GAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTCAGG
    AATAAGATGG TAGAATACTT GACAGATTGG GTTATGGGAA CATCAAACCA AGCGGCAGAT
    GACGATGTAA AATGTCTTAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTGCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAGTT GATGGAAGCC
    AAATCACAGT TGTTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA CGACTGCAGT
    GAAGTCGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCACTGA GGCACTGCAC AGTCCTGGCA ATGTCAAACT TACTCAATGC TAACGTGGAC
    AGCGGCCTCA TGCACTCCAT AGGCTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGCACAG AGTTTGACAC ACTTGCAGAA
    ACAGTATTGG CAGATCGTTT TGAGAGACTG GTGGAGTTGG TCACAATGAT GGGTGATCAA
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTTC CCTGTTCTCA GTGGGATGAA
    CTTGCTCGCG TTCTAGTGAC TCTGTTTGAC TCTCGGCATT TGCTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCTATGC AGACTCTTTT CCGAGGCAAC
    AGCTTGGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAG
    AAACTCCTGG ATCCTTTATT ACGGGTTGTG ATCACCTCCT CTGATTGGCA GCACGTCAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAAAA CCAACGAAAT
    CTCCTTCAAA TGACTGAAAA GTTCTTCCAT GCCATCATCA GTTCGTCCTC CGAATTTCCC
    CCTCAACTAC GAAGTGTGTG CCACTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGTTTCCCT
    CAGAACAGCA TCGGTGCAGT AGGAAGTGCC ATGTTTCTCA GATTTATCAA TCCTGCCATT
    GTCTCACCAT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CGCCTAGAAT CGAAAGGGGC
    TTGAAGTTAA TGTCGAAGAT ACTTCAGAGT ATTGCCAATC ATGTCCTTTT CACAAAAGAA
    GAGCATATGC GGCCTTTTAA TGATTTTGTG AAAAGCAACT TTGATGCAGC ACGAAGGTTT
    TTCCTTGATA TAGCATCTGA TTGTCCTACA AGTGATGCTG TAAATCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGTCTACTAT GGAATAATCA AGAGAAAATT
    GGCCAGTATC TTTCCAGCAA CAGGGATCAC AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCAACAC TTCTTGCATA CCTGGGTCCT CCAGAGCACA AACCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCGAAG TTTGAGGAGT TTATGACTAG GCATCAAGTA
    CATGAAAAAG AAGAGTTCAA GGCTTTGAAA ACATTAAGTA TTTTCTACCA AGCTGGGACT
    TCCAAAGCTG GGAATCCTAT ATTTTATTAT GTTGCACGGA GGTTCAAAAC AGGTCAAATC
    AATGGTGATT TGCTGATATA CCACGTCTTG CTGACTTTAA AGCCATACTA TGCAAAGCCA
    TATGAGATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTT
    CTCTCCAAGT GGTTTGTGGT TTTTCCTGGC TTTGCTTATG ATAATGTCTC TGCAGTATAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAA TATACCAAGT ATCATGAGCG GCTGCTGACT
    GGCCTCAAAG GCAGCAAAAG ACTCATTTTC ATAGACTGTC CTGGGAAACT GGCTGAGCAC
    ATTGAGCATG AACAGCAGAA ACTACCCGCT GCCACCTTGG CTTTAGAAGA GGACCTGAAG
    GTTTTCCACA ACGCTCTCAA GCTAGCTCAC AAAGACACCA AGGTGTCTAT TAAAGTTGGT
    TCTACTGCTG TTCAAGTAAC CTCTGCAGAG CGAACAAAAG TCCTGGGGCA ATCAGTCTTT
    CTAAATGATA TCTATTATGC CTCAGAAATT GAAGAGATCT GCCTGGTGGA CGAGAACCAG
    TTCACCTTAA CTATTGCAAA CCAGGGCACA CCGCTCACCT TCATGCACCA GGAGTGTGAT
    GCCATTGTTC AGTCTATCAT TCACATCCGG ACACGTTGGG AGCTGTCGCA ACCAGACTCC
    ATTCCCCAGC ACACCAAGAT TCGGCCAAAA GACGTCCCTG GGACACTACT CAATATTGCA
    TTACTTAATT TAGGCAGTTC AGACCCTAGT TTACGGTCAG CTGCCTATAA TCTTCTGTGT
    GCCTTAACTT GTACCTTTAA TTTAAAAATT GAGGGCCAGT TACTAGAGAC GTCAGGTTTA
    TGTATCCCAG CCAACAACAC CCTCTTTATT GTCTCTATTA GTAAGACCCT GGCAGCCAAT
    GAGCCACACC TCACCTTAGA ATTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AACACCTTTG TTTGGAGTAC ATGACTCCAT GGCTATCAAA TCTAGTACGT
    TTTTGTAAGC ACAACGATGA TGCCAAGCGA CAGAGAGTTA CTGCAATTCT TGATAAGCTG
    ATAACAATGA CCATAAACGA GAAACAGATG TATCCATCTA TTCAAGCAAA AATATGGGGG
    AGCCTTGGGC AGATTACAGA CCTCCTTGAT GTTGTACTAG ACAGTTTCAT CAAAACCAGT
    GCAACGGGTG GCTTAGGATC AATAAAGGCT GAGGTGATGG CAGATACTGC TGTAGCTTTG
    GCTTCTGGAA ATGTGAAATT GGTTTCAAGC AAGGTTATTG GAAGGATGTG CAAAATAATT
    GACAAGACAT GCCTATCTCC AACTCCTACT TTAGAGCAAC ATCTTATGTG GGATGACATT
    GCCATTCTGG CTCGCTACAT GCTAATGCTG TCCTTCAACA ATTCCCTGGA CGTGGCAGCT
    CATCTCCCCT ACCTCTTCCA CGTTGTTACT TTCTTAGTAG CAACAGGGCC ACTCTCCCTG
    AGAGCTTCCA CACATGGATT GGTCATTAAC ATCATTCACT CTCTGTGTAC TTGTTCACAG
    CTTCATTTTA GTGAAGAGAC CAAGCAAGTT TTGAGACTCA GCTTGACAGA GTTCTCATTA
    CCGAAATTTT ACCTGCTATT TGGCATTAGC AAAGTCAAGT CAGCTGCTGT CATTGCCTTC
    CGCTCCAGCT ACCGGGACAG GTCATTCTCT CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACATCCT TGGAGACAGT CACAGAGGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGACCAA TGGACAGAAC TAGCACAAAG ATTTGCATTC
    CAGTATAACC CGTCCCTGCA ACCGAGAGCT CTTGTTGTCT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGTATCCTCA GCAAGGCTCT TGAGAGTTGC
    TTAAAAGGAC CTGATACTTA CAACAGTCAA GTTCTGATAG AAGCTACAGT AATAGCACTA
    ACCAAATTAC AACCACTTCT TAATAAGGAC TCCCCTCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTAGCCG TGCTACAGCT CGATGAGGTC AACTTGTATT CAGCAGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT AGACAGTCTC AGGATATTCA ACGACAAGAG TCCAGAGGAA
    GTGTTCATGG CAATCCGGAA CCCTCTGGAA TGGCACTGCA AACAAATGGA TCATTTTGTT
    GGACTCAATT TCAACTCTAA CTTTAACTTC GCACTGGTTG GACACCTCTT AAAAGGATAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAGCT
    CTGGTTAATA AACACAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG CGTGGCTTAC
    TTGGCAGCTT TACTTACAGT GTCTGAGGAA GTTCGAAGTC GCTGTAGCCT AAAACATAGA
    AAGTCTCTGC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCATGGTG ACCCTAGCTA TAGGACACTA AAGGAGCATC AGCCGTGGTC ATCTCCCAAA
    GCTTCTGAAG GGTACCTTGC AGCCAGCTAT CCACCTGCGG GCCAGACCAG TCCCCGAGCC
    AGGAAATCTA TGAGCTTGGA CATGGGGCAG CCTTCTCAGG CCAACACGAA GAAATTGCTT
    GGAACAAGGA AAAGTTTTGA TCACTTGATA TCAGACACAA AGGCTCCTAA AAGGCAAGAA
    ATGGAATCAG GGATCACAAC ACCCCCCAAA ATGAGGAGAG TAGCAGAAAC TGATTATGAA
    ATGGAAACCC AAAGGATTTC CTCATCACAG CAGCACCCAC ATTTACGCAA AGTTTCAGTG
    TCTGAATCAA ATGTTCTTTT AGATGAAGAA GTACTTACTG ATCCGAAGAT CCAAGCACTT
    CTTCTTACTG TTCTGGCTAC ACTGGTGAAA TACACCACAG ATGAGTTTGA TCAACGAATT
    CTTTATGAAT ACTTAGCAGA AGCCAGTGTT GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCTAAGAT CAACACCCTG TTGTCATTGT GCCAAGATCC AAATTTGTTA
    AATCCGATCC ATGGAATTGT GCAAAGTGTG GTGTACCATG AAGAATCCCC ACCACAATAC
    CAAACATCTT ACCTGCAAAG TTTTGGTTTT AATGGCTTAT GGAGGTTTGC AGGACCCTTT
    TCAAAGCAAA CACAAATCCC AGACTATGCT GAGCTAATTG TTAAGTTTCT TGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ATCCTCCTGC AGTGCAGAGC CAGCTTAGTA TTACTGCTAA CCTTAACCTT
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGAATCGA CAAGGAGAAT
    GTTGAACTCT CCCCCACCAC TGGGCACTGT AACAGTGGAC GAACTCGCCA TGGATCCGCA
    AGCCAGGTGC AGAAGCAGAG AAGTGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001116200.2
    CDS385..8910
    Translation

    Target ORF information:

    RefSeq Version NM_001122728.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofibromin 1 (NF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001122728.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACTGA GCACAACAAG
    GAATGTCTTA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGTGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCATACTT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTGTTATTTT CTCTTAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAC AATGTGGATG TTCATGATAT AGAACTGTTA
    CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAAGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAGC TGTACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCTGA AAAACTGTTT GACTTAGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTGC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCTAAGGA TGTGGTTGAT GAAAACAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GAAAAGCACT TGCCGGCCAT GGAGGAAGCA GGCAGCTCAC TGAAAGTGCT
    GCAATCGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATCTTTC TGCTGGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT GTTTAATCCG
    AGTAAGCCAT TCTCGAGGGG CAGTCAGCCG GCAGATGTGG ACCTAATGAT CGACTGCCTT
    GTCTCTTGCT TCCGGATAAG CCCTCACAAT AACCAACACT TCAAGATCTG CCTGGCTCAG
    AATTCCCCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GCCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTCCAGGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTACAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CGAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACTGCAG AATTAATTAC AGGGCTTGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCCCAGG AAGCGATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATTTGCAAGA AATTGACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCTTCCT TTATGGAGTA GGATGTGATG TTCCTTCAAG TGGGAATACC
    AGTCAAATGT CCATGGATCA TGACGAATTA CTACGCACTT GTGCTCCTGG AACCTCTCTC
    AGGAAGGGGA AAGGAAATTC ATCCATGGAT AGTGCAGCAG GGTGCAGTGG AACCCCACCA
    ATATGCCGAC AAGCCCAGAC CAAACTAGAA GTGGCCCTGT ACATGTTCCT GTGGAGCCCC
    GACACTGAAG CAGTTCTGGT TGCCATGTCC TGTTTTCGTC ACCTCTGTGA GGAAGCAGAT
    ATCCGGTGTG GGGTGGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CTTCTGTTAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCGC TACTACGGCG AATTGAGCAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACGCATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CACAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTA TCTGACACGG ACTCACTACA AGAATGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCTTAGCAA CATATAGCCC ACCCATGGGC CCTGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CTTCAGAGGG AAATGCAGAT ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTGTCCTTAA TGGTCTGTAA CCATGAGAAA GTGGGACTTC AAATACGGAC CAATGTTAAG
    GATCTGGTGG GTCTAGAATT GAGTCCTGCT CTTTATCCAA TGCTTTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAGTTTGTGG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACCGAAGGC
    AGTTCTGAAC ATCTGGGGCA AGCCAGCATT GAAACAATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGAAATAT GGTTCATGCA ATTCAAATAA AAACAAAACT GTGTCAGTTA
    GTCGAGGTAA TGATGGCAAG GAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTCAGG
    AATAAGATGG TAGAATACTT GACAGATTGG GTTATGGGAA CATCAAACCA AGCGGCAGAT
    GACGATGTAA AATGTCTTAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTGCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAGTT GATGGAAGCC
    AAATCACAGT TGTTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA CGACTGCAGT
    GAAGTCGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCACTGA GGCACTGCAC AGTCCTGGCA ATGTCAAACT TACTCAATGC TAACGTGGAC
    AGCGGCCTCA TGCACTCCAT AGGCTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGCACAG AGTTTGACAC ACTTGCAGAA
    ACAGTATTGG CAGATCGTTT TGAGAGACTG GTGGAGTTGG TCACAATGAT GGGTGATCAA
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTTC CCTGTTCTCA GTGGGATGAA
    CTTGCTCGCG TTCTAGTGAC TCTGTTTGAC TCTCGGCATT TGCTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCTATGC AGACTCTTTT CCGAGGCAAC
    AGCTTGGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAG
    AAACTCCTGG ATCCTTTATT ACGGGTTGTG ATCACCTCCT CTGATTGGCA GCACGTCAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAAAA CCAACGAAAT
    CTCCTTCAAA TGACTGAAAA GTTCTTCCAT GCCATCATCA GTTCGTCCTC CGAATTTCCC
    CCTCAACTAC GAAGTGTGTG CCACTGTTTG TACCAGGCAA CTTGCCACTC CCTACTGAAT
    AAAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGTTTCCCT
    CAGAACAGCA TCGGTGCAGT AGGAAGTGCC ATGTTTCTCA GATTTATCAA TCCTGCCATT
    GTCTCACCAT ATGAAGCAGG GATTTTAGAT AAAAAGCCAC CGCCTAGAAT CGAAAGGGGC
    TTGAAGTTAA TGTCGAAGAT ACTTCAGAGT ATTGCCAATC ATGTCCTTTT CACAAAAGAA
    GAGCATATGC GGCCTTTTAA TGATTTTGTG AAAAGCAACT TTGATGCAGC ACGAAGGTTT
    TTCCTTGATA TAGCATCTGA TTGTCCTACA AGTGATGCTG TAAATCATAG TCTTTCCTTC
    ATCAGCGATG GCAATGTGCT TGCTTTACAT CGTCTACTAT GGAATAATCA AGAGAAAATT
    GGCCAGTATC TTTCCAGCAA CAGGGATCAC AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCAACAC TTCTTGCATA CCTGGGTCCT CCAGAGCACA AACCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCGAAG TTTGAGGAGT TTATGACTAG GCATCAAGTA
    CATGAAAAAG AAGAGTTCAA GGCTTTGAAA ACATTAAGTA TTTTCTACCA AGCTGGGACT
    TCCAAAGCTG GGAATCCTAT ATTTTATTAT GTTGCACGGA GGTTCAAAAC AGGTCAAATC
    AATGGTGATT TGCTGATATA CCACGTCTTG CTGACTTTAA AGCCATACTA TGCAAAGCCA
    TATGAGATTG TAGTGGACCT TACCCATACT GGGCCTAGCA ATCGCTTTAA AACAGACTTT
    CTCTCCAAGT GGTTTGTGGT TTTTCCTGGC TTTGCTTATG ATAATGTCTC TGCAGTATAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAA TATACCAAGT ATCATGAGCG GCTGCTGACT
    GGCCTCAAAG GCAGCAAAAG ACTCATTTTC ATAGACTGTC CTGGGAAACT GGCTGAGCAC
    ATTGAGCATG AACAGCAGAA ACTACCCGCT GCCACCTTGG CTTTAGAAGA GGACCTGAAG
    GTTTTCCACA ACGCTCTCAA GCTAGCTCAC AAAGACACCA AGGTGTCTAT TAAAGTTGGT
    TCTACTGCTG TTCAAGTAAC CTCTGCAGAG CGAACAAAAG TCCTGGGGCA ATCAGTCTTT
    CTAAATGATA TCTATTATGC CTCAGAAATT GAAGAGATCT GCCTGGTGGA CGAGAACCAG
    TTCACCTTAA CTATTGCAAA CCAGGGCACA CCGCTCACCT TCATGCACCA GGAGTGTGAT
    GCCATTGTTC AGTCTATCAT TCACATCCGG ACACGTTGGG AGCTGTCGCA ACCAGACTCC
    ATTCCCCAGC ACACCAAGAT TCGGCCAAAA GACGTCCCTG GGACACTACT CAATATTGCA
    TTACTTAATT TAGGCAGTTC AGACCCTAGT TTACGGTCAG CTGCCTATAA TCTTCTGTGT
    GCCTTAACTT GTACCTTTAA TTTAAAAATT GAGGGCCAGT TACTAGAGAC GTCAGGTTTA
    TGTATCCCAG CCAACAACAC CCTCTTTATT GTCTCTATTA GTAAGACCCT GGCAGCCAAT
    GAGCCACACC TCACCTTAGA ATTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAATTGA AACACCTTTG TTTGGAGTAC ATGACTCCAT GGCTATCAAA TCTAGTACGT
    TTTTGTAAGC ACAACGATGA TGCCAAGCGA CAGAGAGTTA CTGCAATTCT TGATAAGCTG
    ATAACAATGA CCATAAACGA GAAACAGATG TATCCATCTA TTCAAGCAAA AATATGGGGG
    AGCCTTGGGC AGATTACAGA CCTCCTTGAT GTTGTACTAG ACAGTTTCAT CAAAACCAGT
    GCAACGGGTG GCTTAGGATC AATAAAGGCT GAGGTGATGG CAGATACTGC TGTAGCTTTG
    GCTTCTGGAA ATGTGAAATT GGTTTCAAGC AAGGTTATTG GAAGGATGTG CAAAATAATT
    GACAAGACAT GCCTATCTCC AACTCCTACT TTAGAGCAAC ATCTTATGTG GGATGACATT
    GCCATTCTGG CTCGCTACAT GCTAATGCTG TCCTTCAACA ATTCCCTGGA CGTGGCAGCT
    CATCTCCCCT ACCTCTTCCA CGTTGTTACT TTCTTAGTAG CAACAGGGCC ACTCTCCCTG
    AGAGCTTCCA CACATGGATT GGTCATTAAC ATCATTCACT CTCTGTGTAC TTGTTCACAG
    CTTCATTTTA GTGAAGAGAC CAAGCAAGTT TTGAGACTCA GCTTGACAGA GTTCTCATTA
    CCGAAATTTT ACCTGCTATT TGGCATTAGC AAAGTCAAGT CAGCTGCTGT CATTGCCTTC
    CGCTCCAGCT ACCGGGACAG GTCATTCTCT CCTGGCTCCT ATGAGAGGGA GACTTTTGCT
    TTGACATCCT TGGAGACAGT CACAGAGGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGACCAA TGGACAGAAC TAGCACAAAG ATTTGCATTC
    CAGTATAACC CGTCCCTGCA ACCGAGAGCT CTTGTTGTCT TTGGCTGTAT TAGCAAACGA
    GTGTCTCATG GGCAGATAAA GCAGATTATC CGTATCCTCA GCAAGGCTCT TGAGAGTTGC
    TTAAAAGGAC CTGATACTTA CAACAGTCAA GTTCTGATAG AAGCTACAGT AATAGCACTA
    ACCAAATTAC AACCACTTCT TAATAAGGAC TCCCCTCTGC ACAAAGCCCT CTTTTGGGTT
    GCTGTAGCCG TGCTACAGCT CGATGAGGTC AACTTGTATT CAGCAGGCAC TGCACTTCTG
    GAACAAAACC TGCACACCTT AGACAGTCTC AGGATATTCA ACGACAAGAG TCCAGAGGAA
    GTGTTCATGG CAATCCGGAA CCCTCTGGAA TGGCACTGCA AACAAATGGA TCATTTTGTT
    GGACTCAATT TCAACTCTAA CTTTAACTTC GCACTGGTTG GACACCTCTT AAAAGGATAC
    AGGCATCCTT CACCTGCCAT TGTTGCAAGA ACAGTCAGAA TTTTGCATAC ACTACTAGCT
    CTGGTTAATA AACACAGAAA TTGTGACAAA TTTGAGGTGA ATACACAGAG CGTGGCTTAC
    TTGGCAGCTT TACTTACAGT GTCTGAGGAA GTTCGAAGTC GCTGTAGCCT AAAACATAGA
    AAGTCTCTGC TTCTTACTGA TATTTCAATG GAAAATGTTC CTATGGATAC ATATCCCATT
    CATCATGGTG ACCCTAGCTA TAGGACACTA AAGGAGCATC AGCCGTGGTC ATCTCCCAAA
    GCTTCTGAAG GGTACCTTGC AGCCAGCTAT CCACCTGCGG GCCAGACCAG TCCCCGAGCC
    AGGAAATCTA TGAGCTTGGA CATGGGGCAG CCTTCTCAGG CCAACACGAA GAAATTGCTT
    GGAACAAGGA AAAGTTTTGA TCACTTGATA TCAGACACAA AGGCTCCTAA AAGGCAAGAA
    ATGGAATCAG GGATCACAAC ACCCCCCAAA ATGAGGAGAG TAGCAGAAAC TGATTATGAA
    ATGGAAACCC AAAGGATTTC CTCATCACAG CAGCACCCAC ATTTACGCAA AGTTTCAGTG
    TCTGAATCAA ATGTTCTTTT AGATGAAGAA GTACTTACTG ATCCGAAGAT CCAAGCACTT
    CTTCTTACTG TTCTGGCTAC ACTGGTGAAA TACACCACAG ATGAGTTTGA TCAACGAATT
    CTTTATGAAT ACTTAGCAGA AGCCAGTGTT GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCTAAGAT CAACACCCTG TTGTCATTGT GCCAAGATCC AAATTTGTTA
    AATCCGATCC ATGGAATTGT GCAAAGTGTG GTGTACCATG AAGAATCCCC ACCACAATAC
    CAAACATCTT ACCTGCAAAG TTTTGGTTTT AATGGCTTAT GGAGGTTTGC AGGACCCTTT
    TCAAAGCAAA CACAAATCCC AGACTATGCT GAGCTAATTG TTAAGTTTCT TGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ATCCTCCTGC AGTGCAGAGC CAGCTTAGTA TTACTGCTAA CCTTAACCTT
    TCTAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGAATCGA CAAGGAGAAT
    GTTGAACTCT CCCCCACCAC TGGGCACTGT AACAGTGGAC GAACTCGCCA TGGATCCGCA
    AGCCAGGTGC AGAAGCAGAG AAGTGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa261933
    Clone ID Related Accession (Same CDS sequence) NM_001372007.1
    Accession Version NM_001372007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-30
    Organism Bos taurus(cattle)
    Product neurofibromin isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000019.1. Transcript Variant: This variant (2) lacks an internal in-frame exon in the coding region compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACTGA GCACAACAAG
    GAATGTCTTA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGTGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCATACTT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTGTTATTTT CTCTTAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAC AATGTGGATG TTCATGATAT AGAACTGTTA
    CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAAGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAGC TGTACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCTGA AAAACTGTTT GACTTAGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTGC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCTAAGGA TGTGGTTGAT GAAAACAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GAAAAGCACT TGCCGGCCAT GGAGGAAGCA GGCAGCTCAC TGAAAGTGCT
    GCAATCGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATCTTTC TGCTGGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT GTTTAATCCG
    AGTAAGCCAT TCTCGAGGGG CAGTCAGCCG GCAGATGTGG ACCTAATGAT CGACTGCCTT
    GTCTCTTGCT TCCGGATAAG CCCTCACAAT AACCAACACT TCAAGATCTG CCTGGCTCAG
    AATTCCCCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GCCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTCCAGGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTACAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CGAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACTGCAG AATTAATTAC AGGGCTTGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCCCAGG AAGCGATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATTTGCAAGA AATTGACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCTTCCT TTATGGAGTA GGATGTGATG TTCCTTCAAG TGGGAATACC
    AGTCAAATGT CCATGGATCA TGACGAATTA CTACGCACTT GTGCTCCTGG AACCTCTCTC
    AGGAAGGGGA AAGGAAATTC ATCCATGGAT AGTGCAGCAG GGTGCAGTGG AACCCCACCA
    ATATGCCGAC AAGCCCAGAC CAAACTAGAA GTGGCCCTGT ACATGTTCCT GTGGAGCCCC
    GACACTGAAG CAGTTCTGGT TGCCATGTCC TGTTTTCGTC ACCTCTGTGA GGAAGCAGAT
    ATCCGGTGTG GGGTGGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CTTCTGTTAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCGC TACTACGGCG AATTGAGCAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACGCATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CACAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTA TCTGACACGG ACTCACTACA AGAATGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCTTAGCAA CATATAGCCC ACCCATGGGC CCTGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CTTCAGAGGG AAATGCAGAT ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTGTCCTTAA TGGTCTGTAA CCATGAGAAA GTGGGACTTC AAATACGGAC CAATGTTAAG
    GATCTGGTGG GTCTAGAATT GAGTCCTGCT CTTTATCCAA TGCTTTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAGTTTGTGG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACCGAAGGC
    AGTTCTGAAC ATCTGGGGCA AGCCAGCATT GAAACAATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGAAATAT GGTTCATGCA ATTCAAATAA AAACAAAACT GTGTCAGTTA
    GTCGAGGTAA TGATGGCAAG GAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTCAGG
    AATAAGATGG TAGAATACTT GACAGATTGG GTTATGGGAA CATCAAACCA AGCGGCAGAT
    GACGATGTAA AATGTCTTAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTGCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAGTT GATGGAAGCC
    AAATCACAGT TGTTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA CGACTGCAGT
    GAAGTCGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCACTGA GGCACTGCAC AGTCCTGGCA ATGTCAAACT TACTCAATGC TAACGTGGAC
    AGCGGCCTCA TGCACTCCAT AGGCTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGCACAG AGTTTGACAC ACTTGCAGAA
    ACAGTATTGG CAGATCGTTT TGAGAGACTG GTGGAGTTGG TCACAATGAT GGGTGATCAA
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTTC CCTGTTCTCA GTGGGATGAA
    CTTGCTCGCG TTCTAGTGAC TCTGTTTGAC TCTCGGCATT TGCTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCTATGC AGACTCTTTT CCGAGGCAAC
    AGCTTGGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAG
    AAACTCCTGG ATCCTTTATT ACGGGTTGTG ATCACCTCCT CTGATTGGCA GCACGTCAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAAAA CCAACGAAAT
    CTCCTTCAAA TGACTGAAAA GTTCTTCCAT GCCATCATCA GTTCGTCCTC CGAATTTCCC
    CCTCAACTAC GAAGTGTGTG CCACTGTTTG TACCAGGTGG TTAGCCAGCG TTTCCCTCAG
    AACAGCATCG GTGCAGTAGG AAGTGCCATG TTTCTCAGAT TTATCAATCC TGCCATTGTC
    TCACCATATG AAGCAGGGAT TTTAGATAAA AAGCCACCGC CTAGAATCGA AAGGGGCTTG
    AAGTTAATGT CGAAGATACT TCAGAGTATT GCCAATCATG TCCTTTTCAC AAAAGAAGAG
    CATATGCGGC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ATGCAGCACG AAGGTTTTTC
    CTTGATATAG CATCTGATTG TCCTACAAGT GATGCTGTAA ATCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGT CTACTATGGA ATAATCAAGA GAAAATTGGC
    CAGTATCTTT CCAGCAACAG GGATCACAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCAACACTTC TTGCATACCT GGGTCCTCCA GAGCACAAAC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCGAAGTTT GAGGAGTTTA TGACTAGGCA TCAAGTACAT
    GAAAAAGAAG AGTTCAAGGC TTTGAAAACA TTAAGTATTT TCTACCAAGC TGGGACTTCC
    AAAGCTGGGA ATCCTATATT TTATTATGTT GCACGGAGGT TCAAAACAGG TCAAATCAAT
    GGTGATTTGC TGATATACCA CGTCTTGCTG ACTTTAAAGC CATACTATGC AAAGCCATAT
    GAGATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTTCTC
    TCCAAGTGGT TTGTGGTTTT TCCTGGCTTT GCTTATGATA ATGTCTCTGC AGTATATATC
    TATAACTGTA ACTCCTGGGT CAGGGAATAT ACCAAGTATC ATGAGCGGCT GCTGACTGGC
    CTCAAAGGCA GCAAAAGACT CATTTTCATA GACTGTCCTG GGAAACTGGC TGAGCACATT
    GAGCATGAAC AGCAGAAACT ACCCGCTGCC ACCTTGGCTT TAGAAGAGGA CCTGAAGGTT
    TTCCACAACG CTCTCAAGCT AGCTCACAAA GACACCAAGG TGTCTATTAA AGTTGGTTCT
    ACTGCTGTTC AAGTAACCTC TGCAGAGCGA ACAAAAGTCC TGGGGCAATC AGTCTTTCTA
    AATGATATCT ATTATGCCTC AGAAATTGAA GAGATCTGCC TGGTGGACGA GAACCAGTTC
    ACCTTAACTA TTGCAAACCA GGGCACACCG CTCACCTTCA TGCACCAGGA GTGTGATGCC
    ATTGTTCAGT CTATCATTCA CATCCGGACA CGTTGGGAGC TGTCGCAACC AGACTCCATT
    CCCCAGCACA CCAAGATTCG GCCAAAAGAC GTCCCTGGGA CACTACTCAA TATTGCATTA
    CTTAATTTAG GCAGTTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACTTGTA CCTTTAATTT AAAAATTGAG GGCCAGTTAC TAGAGACGTC AGGTTTATGT
    ATCCCAGCCA ACAACACCCT CTTTATTGTC TCTATTAGTA AGACCCTGGC AGCCAATGAG
    CCACACCTCA CCTTAGAATT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAAC ACCTTTGTTT GGAGTACATG ACTCCATGGC TATCAAATCT AGTACGTTTT
    TGTAAGCACA ACGATGATGC CAAGCGACAG AGAGTTACTG CAATTCTTGA TAAGCTGATA
    ACAATGACCA TAAACGAGAA ACAGATGTAT CCATCTATTC AAGCAAAAAT ATGGGGGAGC
    CTTGGGCAGA TTACAGACCT CCTTGATGTT GTACTAGACA GTTTCATCAA AACCAGTGCA
    ACGGGTGGCT TAGGATCAAT AAAGGCTGAG GTGATGGCAG ATACTGCTGT AGCTTTGGCT
    TCTGGAAATG TGAAATTGGT TTCAAGCAAG GTTATTGGAA GGATGTGCAA AATAATTGAC
    AAGACATGCC TATCTCCAAC TCCTACTTTA GAGCAACATC TTATGTGGGA TGACATTGCC
    ATTCTGGCTC GCTACATGCT AATGCTGTCC TTCAACAATT CCCTGGACGT GGCAGCTCAT
    CTCCCCTACC TCTTCCACGT TGTTACTTTC TTAGTAGCAA CAGGGCCACT CTCCCTGAGA
    GCTTCCACAC ATGGATTGGT CATTAACATC ATTCACTCTC TGTGTACTTG TTCACAGCTT
    CATTTTAGTG AAGAGACCAA GCAAGTTTTG AGACTCAGCT TGACAGAGTT CTCATTACCG
    AAATTTTACC TGCTATTTGG CATTAGCAAA GTCAAGTCAG CTGCTGTCAT TGCCTTCCGC
    TCCAGCTACC GGGACAGGTC ATTCTCTCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACATCCTTGG AGACAGTCAC AGAGGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGACCAATGG ACAGAACTAG CACAAAGATT TGCATTCCAG
    TATAACCCGT CCCTGCAACC GAGAGCTCTT GTTGTCTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGT ATCCTCAGCA AGGCTCTTGA GAGTTGCTTA
    AAAGGACCTG ATACTTACAA CAGTCAAGTT CTGATAGAAG CTACAGTAAT AGCACTAACC
    AAATTACAAC CACTTCTTAA TAAGGACTCC CCTCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTAGCCGTGC TACAGCTCGA TGAGGTCAAC TTGTATTCAG CAGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTAGA CAGTCTCAGG ATATTCAACG ACAAGAGTCC AGAGGAAGTG
    TTCATGGCAA TCCGGAACCC TCTGGAATGG CACTGCAAAC AAATGGATCA TTTTGTTGGA
    CTCAATTTCA ACTCTAACTT TAACTTCGCA CTGGTTGGAC ACCTCTTAAA AGGATACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAGCTCTG
    GTTAATAAAC ACAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGCGT GGCTTACTTG
    GCAGCTTTAC TTACAGTGTC TGAGGAAGTT CGAAGTCGCT GTAGCCTAAA ACATAGAAAG
    TCTCTGCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CATGGTGACC CTAGCTATAG GACACTAAAG GAGCATCAGC CGTGGTCATC TCCCAAAGCT
    TCTGAAGGGT ACCTTGCAGC CAGCTATCCA CCTGCGGGCC AGACCAGTCC CCGAGCCAGG
    AAATCTATGA GCTTGGACAT GGGGCAGCCT TCTCAGGCCA ACACGAAGAA ATTGCTTGGA
    ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACAAAGG CTCCTAAAAG GCAAGAAATG
    GAATCAGGGA TCACAACACC CCCCAAAATG AGGAGAGTAG CAGAAACTGA TTATGAAATG
    GAAACCCAAA GGATTTCCTC ATCACAGCAG CACCCACATT TACGCAAAGT TTCAGTGTCT
    GAATCAAATG TTCTTTTAGA TGAAGAAGTA CTTACTGATC CGAAGATCCA AGCACTTCTT
    CTTACTGTTC TGGCTACACT GGTGAAATAC ACCACAGATG AGTTTGATCA ACGAATTCTT
    TATGAATACT TAGCAGAAGC CAGTGTTGTT TTCCCCAAAG TTTTTCCTGT TGTGCACAAT
    TTGTTGGACT CTAAGATCAA CACCCTGTTG TCATTGTGCC AAGATCCAAA TTTGTTAAAT
    CCGATCCATG GAATTGTGCA AAGTGTGGTG TACCATGAAG AATCCCCACC ACAATACCAA
    ACATCTTACC TGCAAAGTTT TGGTTTTAAT GGCTTATGGA GGTTTGCAGG ACCCTTTTCA
    AAGCAAACAC AAATCCCAGA CTATGCTGAG CTAATTGTTA AGTTTCTTGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTATC CTCCTGCAGT GCAGAGCCAG CTTAGTATTA CTGCTAACCT TAACCTTTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAATGTT
    GAACTCTCCC CCACCACTGG GCACTGTAAC AGTGGACGAA CTCGCCATGG ATCCGCAAGC
    CAGGTGCAGA AGCAGAGAAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358936.1
    CDS385..8847
    Translation

    Target ORF information:

    RefSeq Version NM_001372007.1
    Organism Bos taurus(cattle)
    Definition Bos taurus neurofibromin 1 (NF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001372007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGCACTGA GCACAACAAG
    GAATGTCTTA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGTGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTGTAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCATACTT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTGTTATTTT CTCTTAGCTG CAACAACTTC AATGCAGTCT TTAGTCGAAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAC AATGTGGATG TTCATGATAT AGAACTGTTA
    CAGTATATTA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAAGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAGC TGTACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCTGA AAAACTGTTT GACTTAGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTGC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCTAAGGA TGTGGTTGAT GAAAACAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GAAAAGCACT TGCCGGCCAT GGAGGAAGCA GGCAGCTCAC TGAAAGTGCT
    GCAATCGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATCTTTC TGCTGGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT GTTTAATCCG
    AGTAAGCCAT TCTCGAGGGG CAGTCAGCCG GCAGATGTGG ACCTAATGAT CGACTGCCTT
    GTCTCTTGCT TCCGGATAAG CCCTCACAAT AACCAACACT TCAAGATCTG CCTGGCTCAG
    AATTCCCCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTATATT GCCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTCCAGGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTACAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CGAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACTGCAG AATTAATTAC AGGGCTTGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCCCAGG AAGCGATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATTTGCAAGA AATTGACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCTTCCT TTATGGAGTA GGATGTGATG TTCCTTCAAG TGGGAATACC
    AGTCAAATGT CCATGGATCA TGACGAATTA CTACGCACTT GTGCTCCTGG AACCTCTCTC
    AGGAAGGGGA AAGGAAATTC ATCCATGGAT AGTGCAGCAG GGTGCAGTGG AACCCCACCA
    ATATGCCGAC AAGCCCAGAC CAAACTAGAA GTGGCCCTGT ACATGTTCCT GTGGAGCCCC
    GACACTGAAG CAGTTCTGGT TGCCATGTCC TGTTTTCGTC ACCTCTGTGA GGAAGCAGAT
    ATCCGGTGTG GGGTGGATGA AGTGTCAGTA CACAACTTCT TGCCCAACTA TAACACATTC
    ATGGAATTTG CTTCTGTTAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCGC TACTACGGCG AATTGAGCAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACGCATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTACCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CACAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTA TCTGACACGG ACTCACTACA AGAATGGATC
    AACATGACTG GCTTCCTTTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCTTAGCAA CATATAGCCC ACCCATGGGC CCTGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CTTCAGAGGG AAATGCAGAT ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTGTCCTTAA TGGTCTGTAA CCATGAGAAA GTGGGACTTC AAATACGGAC CAATGTTAAG
    GATCTGGTGG GTCTAGAATT GAGTCCTGCT CTTTATCCAA TGCTTTTTAA CAAACTGAAG
    AATACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAGTTTGTGG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACCGAAGGC
    AGTTCTGAAC ATCTGGGGCA AGCCAGCATT GAAACAATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGAAATAT GGTTCATGCA ATTCAAATAA AAACAAAACT GTGTCAGTTA
    GTCGAGGTAA TGATGGCAAG GAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTCAGG
    AATAAGATGG TAGAATACTT GACAGATTGG GTTATGGGAA CATCAAACCA AGCGGCAGAT
    GACGATGTAA AATGTCTTAC AAGAGATTTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTGCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAGTT GATGGAAGCC
    AAATCACAGT TGTTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA CGACTGCAGT
    GAAGTCGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAAC GTGGCATGTC TCGGAGGCTG
    GCATCACTGA GGCACTGCAC AGTCCTGGCA ATGTCAAACT TACTCAATGC TAACGTGGAC
    AGCGGCCTCA TGCACTCCAT AGGCTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACG
    TTTATGGAAG TTCTGACAAA AATCCTTCAA CAAGGCACAG AGTTTGACAC ACTTGCAGAA
    ACAGTATTGG CAGATCGTTT TGAGAGACTG GTGGAGTTGG TCACAATGAT GGGTGATCAA
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTTC CCTGTTCTCA GTGGGATGAA
    CTTGCTCGCG TTCTAGTGAC TCTGTTTGAC TCTCGGCATT TGCTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCTATGC AGACTCTTTT CCGAGGCAAC
    AGCTTGGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAG
    AAACTCCTGG ATCCTTTATT ACGGGTTGTG ATCACCTCCT CTGATTGGCA GCACGTCAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAAAA CCAACGAAAT
    CTCCTTCAAA TGACTGAAAA GTTCTTCCAT GCCATCATCA GTTCGTCCTC CGAATTTCCC
    CCTCAACTAC GAAGTGTGTG CCACTGTTTG TACCAGGTGG TTAGCCAGCG TTTCCCTCAG
    AACAGCATCG GTGCAGTAGG AAGTGCCATG TTTCTCAGAT TTATCAATCC TGCCATTGTC
    TCACCATATG AAGCAGGGAT TTTAGATAAA AAGCCACCGC CTAGAATCGA AAGGGGCTTG
    AAGTTAATGT CGAAGATACT TCAGAGTATT GCCAATCATG TCCTTTTCAC AAAAGAAGAG
    CATATGCGGC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ATGCAGCACG AAGGTTTTTC
    CTTGATATAG CATCTGATTG TCCTACAAGT GATGCTGTAA ATCATAGTCT TTCCTTCATC
    AGCGATGGCA ATGTGCTTGC TTTACATCGT CTACTATGGA ATAATCAAGA GAAAATTGGC
    CAGTATCTTT CCAGCAACAG GGATCACAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCAACACTTC TTGCATACCT GGGTCCTCCA GAGCACAAAC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCGAAGTTT GAGGAGTTTA TGACTAGGCA TCAAGTACAT
    GAAAAAGAAG AGTTCAAGGC TTTGAAAACA TTAAGTATTT TCTACCAAGC TGGGACTTCC
    AAAGCTGGGA ATCCTATATT TTATTATGTT GCACGGAGGT TCAAAACAGG TCAAATCAAT
    GGTGATTTGC TGATATACCA CGTCTTGCTG ACTTTAAAGC CATACTATGC AAAGCCATAT
    GAGATTGTAG TGGACCTTAC CCATACTGGG CCTAGCAATC GCTTTAAAAC AGACTTTCTC
    TCCAAGTGGT TTGTGGTTTT TCCTGGCTTT GCTTATGATA ATGTCTCTGC AGTATATATC
    TATAACTGTA ACTCCTGGGT CAGGGAATAT ACCAAGTATC ATGAGCGGCT GCTGACTGGC
    CTCAAAGGCA GCAAAAGACT CATTTTCATA GACTGTCCTG GGAAACTGGC TGAGCACATT
    GAGCATGAAC AGCAGAAACT ACCCGCTGCC ACCTTGGCTT TAGAAGAGGA CCTGAAGGTT
    TTCCACAACG CTCTCAAGCT AGCTCACAAA GACACCAAGG TGTCTATTAA AGTTGGTTCT
    ACTGCTGTTC AAGTAACCTC TGCAGAGCGA ACAAAAGTCC TGGGGCAATC AGTCTTTCTA
    AATGATATCT ATTATGCCTC AGAAATTGAA GAGATCTGCC TGGTGGACGA GAACCAGTTC
    ACCTTAACTA TTGCAAACCA GGGCACACCG CTCACCTTCA TGCACCAGGA GTGTGATGCC
    ATTGTTCAGT CTATCATTCA CATCCGGACA CGTTGGGAGC TGTCGCAACC AGACTCCATT
    CCCCAGCACA CCAAGATTCG GCCAAAAGAC GTCCCTGGGA CACTACTCAA TATTGCATTA
    CTTAATTTAG GCAGTTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACTTGTA CCTTTAATTT AAAAATTGAG GGCCAGTTAC TAGAGACGTC AGGTTTATGT
    ATCCCAGCCA ACAACACCCT CTTTATTGTC TCTATTAGTA AGACCCTGGC AGCCAATGAG
    CCACACCTCA CCTTAGAATT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAATTGAAAC ACCTTTGTTT GGAGTACATG ACTCCATGGC TATCAAATCT AGTACGTTTT
    TGTAAGCACA ACGATGATGC CAAGCGACAG AGAGTTACTG CAATTCTTGA TAAGCTGATA
    ACAATGACCA TAAACGAGAA ACAGATGTAT CCATCTATTC AAGCAAAAAT ATGGGGGAGC
    CTTGGGCAGA TTACAGACCT CCTTGATGTT GTACTAGACA GTTTCATCAA AACCAGTGCA
    ACGGGTGGCT TAGGATCAAT AAAGGCTGAG GTGATGGCAG ATACTGCTGT AGCTTTGGCT
    TCTGGAAATG TGAAATTGGT TTCAAGCAAG GTTATTGGAA GGATGTGCAA AATAATTGAC
    AAGACATGCC TATCTCCAAC TCCTACTTTA GAGCAACATC TTATGTGGGA TGACATTGCC
    ATTCTGGCTC GCTACATGCT AATGCTGTCC TTCAACAATT CCCTGGACGT GGCAGCTCAT
    CTCCCCTACC TCTTCCACGT TGTTACTTTC TTAGTAGCAA CAGGGCCACT CTCCCTGAGA
    GCTTCCACAC ATGGATTGGT CATTAACATC ATTCACTCTC TGTGTACTTG TTCACAGCTT
    CATTTTAGTG AAGAGACCAA GCAAGTTTTG AGACTCAGCT TGACAGAGTT CTCATTACCG
    AAATTTTACC TGCTATTTGG CATTAGCAAA GTCAAGTCAG CTGCTGTCAT TGCCTTCCGC
    TCCAGCTACC GGGACAGGTC ATTCTCTCCT GGCTCCTATG AGAGGGAGAC TTTTGCTTTG
    ACATCCTTGG AGACAGTCAC AGAGGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGACCAATGG ACAGAACTAG CACAAAGATT TGCATTCCAG
    TATAACCCGT CCCTGCAACC GAGAGCTCTT GTTGTCTTTG GCTGTATTAG CAAACGAGTG
    TCTCATGGGC AGATAAAGCA GATTATCCGT ATCCTCAGCA AGGCTCTTGA GAGTTGCTTA
    AAAGGACCTG ATACTTACAA CAGTCAAGTT CTGATAGAAG CTACAGTAAT AGCACTAACC
    AAATTACAAC CACTTCTTAA TAAGGACTCC CCTCTGCACA AAGCCCTCTT TTGGGTTGCT
    GTAGCCGTGC TACAGCTCGA TGAGGTCAAC TTGTATTCAG CAGGCACTGC ACTTCTGGAA
    CAAAACCTGC ACACCTTAGA CAGTCTCAGG ATATTCAACG ACAAGAGTCC AGAGGAAGTG
    TTCATGGCAA TCCGGAACCC TCTGGAATGG CACTGCAAAC AAATGGATCA TTTTGTTGGA
    CTCAATTTCA ACTCTAACTT TAACTTCGCA CTGGTTGGAC ACCTCTTAAA AGGATACAGG
    CATCCTTCAC CTGCCATTGT TGCAAGAACA GTCAGAATTT TGCATACACT ACTAGCTCTG
    GTTAATAAAC ACAGAAATTG TGACAAATTT GAGGTGAATA CACAGAGCGT GGCTTACTTG
    GCAGCTTTAC TTACAGTGTC TGAGGAAGTT CGAAGTCGCT GTAGCCTAAA ACATAGAAAG
    TCTCTGCTTC TTACTGATAT TTCAATGGAA AATGTTCCTA TGGATACATA TCCCATTCAT
    CATGGTGACC CTAGCTATAG GACACTAAAG GAGCATCAGC CGTGGTCATC TCCCAAAGCT
    TCTGAAGGGT ACCTTGCAGC CAGCTATCCA CCTGCGGGCC AGACCAGTCC CCGAGCCAGG
    AAATCTATGA GCTTGGACAT GGGGCAGCCT TCTCAGGCCA ACACGAAGAA ATTGCTTGGA
    ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACAAAGG CTCCTAAAAG GCAAGAAATG
    GAATCAGGGA TCACAACACC CCCCAAAATG AGGAGAGTAG CAGAAACTGA TTATGAAATG
    GAAACCCAAA GGATTTCCTC ATCACAGCAG CACCCACATT TACGCAAAGT TTCAGTGTCT
    GAATCAAATG TTCTTTTAGA TGAAGAAGTA CTTACTGATC CGAAGATCCA AGCACTTCTT
    CTTACTGTTC TGGCTACACT GGTGAAATAC ACCACAGATG AGTTTGATCA ACGAATTCTT
    TATGAATACT TAGCAGAAGC CAGTGTTGTT TTCCCCAAAG TTTTTCCTGT TGTGCACAAT
    TTGTTGGACT CTAAGATCAA CACCCTGTTG TCATTGTGCC AAGATCCAAA TTTGTTAAAT
    CCGATCCATG GAATTGTGCA AAGTGTGGTG TACCATGAAG AATCCCCACC ACAATACCAA
    ACATCTTACC TGCAAAGTTT TGGTTTTAAT GGCTTATGGA GGTTTGCAGG ACCCTTTTCA
    AAGCAAACAC AAATCCCAGA CTATGCTGAG CTAATTGTTA AGTTTCTTGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTATC CTCCTGCAGT GCAGAGCCAG CTTAGTATTA CTGCTAACCT TAACCTTTCT
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAATGTT
    GAACTCTCCC CCACCACTGG GCACTGTAAC AGTGGACGAA CTCGCCATGG ATCCGCAAGC
    CAGGTGCAGA AGCAGAGAAG TGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.