MTR cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR
Entrez Gene ID	280869
Full Name	5-methyltetrahydrofolate-homocysteine methyltransferase
General protein information	Preferred Names 5-methyltetrahydrofolate-homocysteine methyltransferase Names methionine synthase MS vitamin-B12 dependent methionine synthase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015460920.1	XP_015316406.1	methionine synthase isoform X2
XM_010820413.1	XP_010818715.1	methionine synthase isoform X3
XM_024986502.1	XP_024842270.1	methionine synthase isoform X2
XM_024986504.1	XP_024842272.1	methionine synthase isoform X4
NM_001030298.1	NP_001025469.1	methionine synthase
XM_010820411.2	XP_010818713.1	methionine synthase isoform X1
XM_010820411.3	XP_010818713.1	methionine synthase isoform X1
XM_024986503.1	XP_024842271.1	methionine synthase isoform X3

Pathways from BioSystems

Homologies

	MTR	XP_001109238.1
Rattus norvegicus (Norway rat)	Mtr	NP_110491.1
Danio rerio (zebrafish)	mtr	NP_932338.1
Xenopus tropicalis (tropical clawed frog)	LOC100488827	XP_002933914.1
Homo sapiens (human)	MTR	NP_000245.2
Canis lupus familiaris (dog)	MTR	XP_005618839.1
Caenorhabditis elegans (roundworm)	metr-1	NP_496353.1
Pan troglodytes (chimpanzee)	MTR	XP_514294.2
Mus musculus (house mouse)	Mtr	NP_001074597.1
Gallus gallus (chicken)	MTR	NP_001026275.1
Bos taurus (cattle)	MTR	NP_001025469.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatic Activity and Transcription of Betaine-Homocysteine Methyltransferase, Methionine Synthase, and Cystathionine Synthase in Periparturient Dairy Cows Are Altered to Different Extents by Supply of Methionine and Choline.
Zhou Z, Garrow TA, Dong X, Luchini DN, Loor JJ
The Journal of nutrition147(1)11-19(2017 01)

Effects of dietary supplements of folic acid and vitamin B12 on metabolism of dairy cows in early lactation.
Graulet B, Matte JJ, Desrochers A, Doepel L, Palin MF, Girard CL
Journal of dairy science90(7)3442-55(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that greater synthesis of phosphatidylcholine and antioxidants contribute to the better performance and immuno-metabolic status in methionine-supplemented cows.
Title: Hepatic Activity and Transcription of Betaine-Homocysteine Methyltransferase, Methionine Synthase, and Cystathionine Synthase in Periparturient Dairy Cows Are Altered to Different Extents by Supply of Methionine and Choline.

The following MTR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa45031	XM_024986504.1 Latest version!	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa45031	XM_010820413.1 Latest version!	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa231519	XM_024986503.1 Latest version!	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa45030	XM_024986502.1 Latest version!	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBa88869	XM_015460920.1 Latest version!	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa42210	NM_001030298.1 Latest version!	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa45029	XM_010820411.3 Latest version!	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OBa45029	XM_010820411.2	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR ( XM_010820413.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818715.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa45031
Clone ID Related Accession (Same CDS sequence)	XM_010820413.1 , XM_024986504.1
Accession Version	XM_010820413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methionine synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104619.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTGG AAATGGGAGC CCAGGTGTTG GATATTAACA TGGATGATGG CATGCTGGAT 
GGTCCAAGTG CAATGACCAG ATTCTGCAAC TTCATTGCTT CCGAGCCTGA CATTGCCAAG 
GTGCCCTTGT GCATTGACTC TTCCAATTTT GCTGTGATTG AAGCCGGCTT AAAGTGCTGC 
CAGGGGAAGT GCATTGTTAA TAGCATCAGC TTGAAGGAGG GAGAGGATGA CTTCTTGGAG 
AAGGCCAGAA AGATTAAGAA GTTTGGAGCT GCCGTGGTGG TTATGGCTTT TGATGAAGAA 
GGACAGGCAA CAGAAACAGA TACCAAAATC AGAGTGTGCA CCCGGGCCTA CCATCTCCTT 
TTGAAAAAAC TGGGCTTCAA TCCAAATGAC ATCATCTTTG ACCCTAATAT CCTCACCATT 
GGTACTGGGA TGGAAGAACA TAACTTGTAT GCTGTTAATT TTATCAATGC AACAAAAGTC 
ATTAAAGAAA CGCTGCCTGG AGCCAAAGTA AGCGGTGGTC TTTCCAACTT GTCCTTCTCC 
TTCCGAGGAA TGGAAGCCAT TCGAGAGGCA ATGCATGGGG TTTTCCTGTA CCATGCGATC 
AAGTTTGGTA TGGACATGGG AATAGTGAAT GCTGGAAGCC TTCCTGTATA TGATGACATC 
CACAAGGAGC TTCTCCAGCT CTGTGAGGAC CTCATCTGGA ACAGAGACCC TGAGGCCACC 
GAGAAGCTCT TACACTACGC CCAGACTCAG GGCAAAGGAG GGAAGAAAGT CATCCAGACT 
GATGAGTGGA GGAATGGCCC TCTCGAAGAA CGCCTTGAGT ACGCTCTTGT GAAGGGCATT 
GAAAAATATA TTATTGAAGA TACTGAGGAA GCCAGGTTAA ACCAGGAAAA ATATCCCCGA 
CCTCTGAATA TAATTGAAGG GCCGCTGATG AATGGCATGA AAATTGTTGG TGATCTTTTT 
GGAGCTGGAA AAATGTTTCT ACCTCAGGTT ATAAAGTCAG CTCGGGTCAT GAAGAAGGCC 
GTGGGTCACC TTATTCCCTT CATGGAAAAA GAGAGAGAAG AAACCAAAGT GCTTACTGGC 
AAAATAGAAG ATGAGGACCC TTACCAAGGC ACCATCGTGC TGGCTACTGT TAAAGGTGAC 
GTACACGACA TAGGCAAGAA CATAGTTGGA GTGGTTCTTG GCTGCAATAA TTTCAGAGTT 
ATTGATTTAG GAGTCATGAC TCCATGTGAC AAGATACTGA AGGCTGCTCT AGACCACAAA 
GCAGATATAA TTGGCCTGTC AGGACTCATC ACTCCTTCCC TGGATGAAAT GATTTTTGTC 
GCTAAGGAAA TGGAGAGACT AGCTATAAAG ATCCCATTGT TGATCGGAGG AGCTACCACT 
TCCAGAACCC ACACAGCAGT TAAAATAGCC CCAAGATACA GTGCGCCCGT GATCCACGTC 
CTGGATGCAT CCAAGAGCGT GGTGGTGTGT TCTCAACTGT TAGATGAGAA TCTAAAAGAT 
GAGTACTTTG AGGAGATCCT GGAAGAATAT GAAGATATTA GACAGGACCA TTATGAATCT 
CTCAAGGAGA GAAGATACTT AACCTTAAGG CAAGCTAGAG AAAATGGTTT CCATATTGAC 
TGGCTGTCAG AGCCTCCTCC AGTGAAGCCC ACGTTCCTCG GGACTCGGGT CTTTGAAGAC 
TACGACCTGC AGAAGCTGGT GGACTACATT GATTGGAAGC CTTTCTTCGA CGTCTGGCAG 
CTCCGGGGCA AATACCCGAA CCGAGGCTTT CCCAAGATAT TTGACGACAA AACTGTAGGT 
GAAGAGGCCA AAAAGGTCTA TGATGATGCC CAAAATATGC TGCAGGCACT GATCAGTCAG 
AAGAAGCTCC AGGCCAGGGG TGTGGTTGGG TTCTGGCCAG CACAGAGCAT TCAGGATGAT 
ATCCACCTGT ACGCGGAAGG CGCAGTGCCA CAGGCCTCGG AGCCCATAGC CACCTTCTAC 
GGGCTCAGGC AGCAGGCCGA GAAGGACTCT GCCAGCTCGG ACCCCTACCT CTGCCTCTCG 
GACTTCATCG CCCCTCTGCA CTCAGGTATC CCCGACTACC TGGGCCTGTT CGCCGTTGCC 
TGCTTTGGGG TGGAGGAGCT GAGCAAGGCC TACGAGGAGG AGTGTGATGA CTACAGCAGC 
ATCATGGTCA AGGCGCTGGG GGACCGGCTG GCCGAGGCCT TCGCTGAGGA GCTGCACGAG 
CGGGTCCGCA GGGAGCTGTG GGGTTACTGT AGTGGTGAGC AGCTGGCTGT GGCTGACCTG 
CGGAGACTGC GCTACGAGGG CATCCGGCCA GCGCCCGGCT ATCCCAGCCA GCCTGACCAC 
ACTGAGAAGC TGACCATGTG GAGGCTGGCG GACGTGGAGC AGCGCACAGG CATTAGGTTA 
ACAGAATCGC TGGCCATGGC ACCTGCTTCA GCAGTATCTG GCCTTTACTT CTCCAACTTG 
AAGTCCAAGT ATTTTGCCGT GGGGAAAATT TCCAAGGATC AGATTGAGGA TTATGCTTCG 
AGGAAGAACA TGTCTGTGGC CGAGGTTGAG AAATGGCTTG GACCCATTTT GGGATATGAT 
ACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818715.1
CDS	102..2690
Translation	MQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDTKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_010820413.1
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820413.1

ORF Insert Sequence:

ATGCAGGTGG AAATGGGAGC CCAGGTGTTG GATATTAACA TGGATGATGG CATGCTGGAT 
GGTCCAAGTG CAATGACCAG ATTCTGCAAC TTCATTGCTT CCGAGCCTGA CATTGCCAAG 
GTGCCCTTGT GCATTGACTC TTCCAATTTT GCTGTGATTG AAGCCGGCTT AAAGTGCTGC 
CAGGGGAAGT GCATTGTTAA TAGCATCAGC TTGAAGGAGG GAGAGGATGA CTTCTTGGAG 
AAGGCCAGAA AGATTAAGAA GTTTGGAGCT GCCGTGGTGG TTATGGCTTT TGATGAAGAA 
GGACAGGCAA CAGAAACAGA TACCAAAATC AGAGTGTGCA CCCGGGCCTA CCATCTCCTT 
TTGAAAAAAC TGGGCTTCAA TCCAAATGAC ATCATCTTTG ACCCTAATAT CCTCACCATT 
GGTACTGGGA TGGAAGAACA TAACTTGTAT GCTGTTAATT TTATCAATGC AACAAAAGTC 
ATTAAAGAAA CGCTGCCTGG AGCCAAAGTA AGCGGTGGTC TTTCCAACTT GTCCTTCTCC 
TTCCGAGGAA TGGAAGCCAT TCGAGAGGCA ATGCATGGGG TTTTCCTGTA CCATGCGATC 
AAGTTTGGTA TGGACATGGG AATAGTGAAT GCTGGAAGCC TTCCTGTATA TGATGACATC 
CACAAGGAGC TTCTCCAGCT CTGTGAGGAC CTCATCTGGA ACAGAGACCC TGAGGCCACC 
GAGAAGCTCT TACACTACGC CCAGACTCAG GGCAAAGGAG GGAAGAAAGT CATCCAGACT 
GATGAGTGGA GGAATGGCCC TCTCGAAGAA CGCCTTGAGT ACGCTCTTGT GAAGGGCATT 
GAAAAATATA TTATTGAAGA TACTGAGGAA GCCAGGTTAA ACCAGGAAAA ATATCCCCGA 
CCTCTGAATA TAATTGAAGG GCCGCTGATG AATGGCATGA AAATTGTTGG TGATCTTTTT 
GGAGCTGGAA AAATGTTTCT ACCTCAGGTT ATAAAGTCAG CTCGGGTCAT GAAGAAGGCC 
GTGGGTCACC TTATTCCCTT CATGGAAAAA GAGAGAGAAG AAACCAAAGT GCTTACTGGC 
AAAATAGAAG ATGAGGACCC TTACCAAGGC ACCATCGTGC TGGCTACTGT TAAAGGTGAC 
GTACACGACA TAGGCAAGAA CATAGTTGGA GTGGTTCTTG GCTGCAATAA TTTCAGAGTT 
ATTGATTTAG GAGTCATGAC TCCATGTGAC AAGATACTGA AGGCTGCTCT AGACCACAAA 
GCAGATATAA TTGGCCTGTC AGGACTCATC ACTCCTTCCC TGGATGAAAT GATTTTTGTC 
GCTAAGGAAA TGGAGAGACT AGCTATAAAG ATCCCATTGT TGATCGGAGG AGCTACCACT 
TCCAGAACCC ACACAGCAGT TAAAATAGCC CCAAGATACA GTGCGCCCGT GATCCACGTC 
CTGGATGCAT CCAAGAGCGT GGTGGTGTGT TCTCAACTGT TAGATGAGAA TCTAAAAGAT 
GAGTACTTTG AGGAGATCCT GGAAGAATAT GAAGATATTA GACAGGACCA TTATGAATCT 
CTCAAGGAGA GAAGATACTT AACCTTAAGG CAAGCTAGAG AAAATGGTTT CCATATTGAC 
TGGCTGTCAG AGCCTCCTCC AGTGAAGCCC ACGTTCCTCG GGACTCGGGT CTTTGAAGAC 
TACGACCTGC AGAAGCTGGT GGACTACATT GATTGGAAGC CTTTCTTCGA CGTCTGGCAG 
CTCCGGGGCA AATACCCGAA CCGAGGCTTT CCCAAGATAT TTGACGACAA AACTGTAGGT 
GAAGAGGCCA AAAAGGTCTA TGATGATGCC CAAAATATGC TGCAGGCACT GATCAGTCAG 
AAGAAGCTCC AGGCCAGGGG TGTGGTTGGG TTCTGGCCAG CACAGAGCAT TCAGGATGAT 
ATCCACCTGT ACGCGGAAGG CGCAGTGCCA CAGGCCTCGG AGCCCATAGC CACCTTCTAC 
GGGCTCAGGC AGCAGGCCGA GAAGGACTCT GCCAGCTCGG ACCCCTACCT CTGCCTCTCG 
GACTTCATCG CCCCTCTGCA CTCAGGTATC CCCGACTACC TGGGCCTGTT CGCCGTTGCC 
TGCTTTGGGG TGGAGGAGCT GAGCAAGGCC TACGAGGAGG AGTGTGATGA CTACAGCAGC 
ATCATGGTCA AGGCGCTGGG GGACCGGCTG GCCGAGGCCT TCGCTGAGGA GCTGCACGAG 
CGGGTCCGCA GGGAGCTGTG GGGTTACTGT AGTGGTGAGC AGCTGGCTGT GGCTGACCTG 
CGGAGACTGC GCTACGAGGG CATCCGGCCA GCGCCCGGCT ATCCCAGCCA GCCTGACCAC 
ACTGAGAAGC TGACCATGTG GAGGCTGGCG GACGTGGAGC AGCGCACAGG CATTAGGTTA 
ACAGAATCGC TGGCCATGGC ACCTGCTTCA GCAGTATCTG GCCTTTACTT CTCCAACTTG 
AAGTCCAAGT ATTTTGCCGT GGGGAAAATT TCCAAGGATC AGATTGAGGA TTATGCTTCG 
AGGAAGAACA TGTCTGTGGC CGAGGTTGAG AAATGGCTTG GACCCATTTT GGGATATGAT 
ACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR ( XM_024986504.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842272.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa45031
Clone ID Related Accession (Same CDS sequence)	XM_010820413.1 , XM_024986504.1
Accession Version	XM_024986504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methionine synthase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTGG AAATGGGAGC CCAGGTGTTG GATATTAACA TGGATGATGG CATGCTGGAT 
GGTCCAAGTG CAATGACCAG ATTCTGCAAC TTCATTGCTT CCGAGCCTGA CATTGCCAAG 
GTGCCCTTGT GCATTGACTC TTCCAATTTT GCTGTGATTG AAGCCGGCTT AAAGTGCTGC 
CAGGGGAAGT GCATTGTTAA TAGCATCAGC TTGAAGGAGG GAGAGGATGA CTTCTTGGAG 
AAGGCCAGAA AGATTAAGAA GTTTGGAGCT GCCGTGGTGG TTATGGCTTT TGATGAAGAA 
GGACAGGCAA CAGAAACAGA TACCAAAATC AGAGTGTGCA CCCGGGCCTA CCATCTCCTT 
TTGAAAAAAC TGGGCTTCAA TCCAAATGAC ATCATCTTTG ACCCTAATAT CCTCACCATT 
GGTACTGGGA TGGAAGAACA TAACTTGTAT GCTGTTAATT TTATCAATGC AACAAAAGTC 
ATTAAAGAAA CGCTGCCTGG AGCCAAAGTA AGCGGTGGTC TTTCCAACTT GTCCTTCTCC 
TTCCGAGGAA TGGAAGCCAT TCGAGAGGCA ATGCATGGGG TTTTCCTGTA CCATGCGATC 
AAGTTTGGTA TGGACATGGG AATAGTGAAT GCTGGAAGCC TTCCTGTATA TGATGACATC 
CACAAGGAGC TTCTCCAGCT CTGTGAGGAC CTCATCTGGA ACAGAGACCC TGAGGCCACC 
GAGAAGCTCT TACACTACGC CCAGACTCAG GGCAAAGGAG GGAAGAAAGT CATCCAGACT 
GATGAGTGGA GGAATGGCCC TCTCGAAGAA CGCCTTGAGT ACGCTCTTGT GAAGGGCATT 
GAAAAATATA TTATTGAAGA TACTGAGGAA GCCAGGTTAA ACCAGGAAAA ATATCCCCGA 
CCTCTGAATA TAATTGAAGG GCCGCTGATG AATGGCATGA AAATTGTTGG TGATCTTTTT 
GGAGCTGGAA AAATGTTTCT ACCTCAGGTT ATAAAGTCAG CTCGGGTCAT GAAGAAGGCC 
GTGGGTCACC TTATTCCCTT CATGGAAAAA GAGAGAGAAG AAACCAAAGT GCTTACTGGC 
AAAATAGAAG ATGAGGACCC TTACCAAGGC ACCATCGTGC TGGCTACTGT TAAAGGTGAC 
GTACACGACA TAGGCAAGAA CATAGTTGGA GTGGTTCTTG GCTGCAATAA TTTCAGAGTT 
ATTGATTTAG GAGTCATGAC TCCATGTGAC AAGATACTGA AGGCTGCTCT AGACCACAAA 
GCAGATATAA TTGGCCTGTC AGGACTCATC ACTCCTTCCC TGGATGAAAT GATTTTTGTC 
GCTAAGGAAA TGGAGAGACT AGCTATAAAG ATCCCATTGT TGATCGGAGG AGCTACCACT 
TCCAGAACCC ACACAGCAGT TAAAATAGCC CCAAGATACA GTGCGCCCGT GATCCACGTC 
CTGGATGCAT CCAAGAGCGT GGTGGTGTGT TCTCAACTGT TAGATGAGAA TCTAAAAGAT 
GAGTACTTTG AGGAGATCCT GGAAGAATAT GAAGATATTA GACAGGACCA TTATGAATCT 
CTCAAGGAGA GAAGATACTT AACCTTAAGG CAAGCTAGAG AAAATGGTTT CCATATTGAC 
TGGCTGTCAG AGCCTCCTCC AGTGAAGCCC ACGTTCCTCG GGACTCGGGT CTTTGAAGAC 
TACGACCTGC AGAAGCTGGT GGACTACATT GATTGGAAGC CTTTCTTCGA CGTCTGGCAG 
CTCCGGGGCA AATACCCGAA CCGAGGCTTT CCCAAGATAT TTGACGACAA AACTGTAGGT 
GAAGAGGCCA AAAAGGTCTA TGATGATGCC CAAAATATGC TGCAGGCACT GATCAGTCAG 
AAGAAGCTCC AGGCCAGGGG TGTGGTTGGG TTCTGGCCAG CACAGAGCAT TCAGGATGAT 
ATCCACCTGT ACGCGGAAGG CGCAGTGCCA CAGGCCTCGG AGCCCATAGC CACCTTCTAC 
GGGCTCAGGC AGCAGGCCGA GAAGGACTCT GCCAGCTCGG ACCCCTACCT CTGCCTCTCG 
GACTTCATCG CCCCTCTGCA CTCAGGTATC CCCGACTACC TGGGCCTGTT CGCCGTTGCC 
TGCTTTGGGG TGGAGGAGCT GAGCAAGGCC TACGAGGAGG AGTGTGATGA CTACAGCAGC 
ATCATGGTCA AGGCGCTGGG GGACCGGCTG GCCGAGGCCT TCGCTGAGGA GCTGCACGAG 
CGGGTCCGCA GGGAGCTGTG GGGTTACTGT AGTGGTGAGC AGCTGGCTGT GGCTGACCTG 
CGGAGACTGC GCTACGAGGG CATCCGGCCA GCGCCCGGCT ATCCCAGCCA GCCTGACCAC 
ACTGAGAAGC TGACCATGTG GAGGCTGGCG GACGTGGAGC AGCGCACAGG CATTAGGTTA 
ACAGAATCGC TGGCCATGGC ACCTGCTTCA GCAGTATCTG GCCTTTACTT CTCCAACTTG 
AAGTCCAAGT ATTTTGCCGT GGGGAAAATT TCCAAGGATC AGATTGAGGA TTATGCTTCG 
AGGAAGAACA TGTCTGTGGC CGAGGTTGAG AAATGGCTTG GACCCATTTT GGGATATGAT 
ACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842272.1
CDS	131..2719
Translation	MQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDTKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_024986504.1
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986504.1

ORF Insert Sequence:

ATGCAGGTGG AAATGGGAGC CCAGGTGTTG GATATTAACA TGGATGATGG CATGCTGGAT 
GGTCCAAGTG CAATGACCAG ATTCTGCAAC TTCATTGCTT CCGAGCCTGA CATTGCCAAG 
GTGCCCTTGT GCATTGACTC TTCCAATTTT GCTGTGATTG AAGCCGGCTT AAAGTGCTGC 
CAGGGGAAGT GCATTGTTAA TAGCATCAGC TTGAAGGAGG GAGAGGATGA CTTCTTGGAG 
AAGGCCAGAA AGATTAAGAA GTTTGGAGCT GCCGTGGTGG TTATGGCTTT TGATGAAGAA 
GGACAGGCAA CAGAAACAGA TACCAAAATC AGAGTGTGCA CCCGGGCCTA CCATCTCCTT 
TTGAAAAAAC TGGGCTTCAA TCCAAATGAC ATCATCTTTG ACCCTAATAT CCTCACCATT 
GGTACTGGGA TGGAAGAACA TAACTTGTAT GCTGTTAATT TTATCAATGC AACAAAAGTC 
ATTAAAGAAA CGCTGCCTGG AGCCAAAGTA AGCGGTGGTC TTTCCAACTT GTCCTTCTCC 
TTCCGAGGAA TGGAAGCCAT TCGAGAGGCA ATGCATGGGG TTTTCCTGTA CCATGCGATC 
AAGTTTGGTA TGGACATGGG AATAGTGAAT GCTGGAAGCC TTCCTGTATA TGATGACATC 
CACAAGGAGC TTCTCCAGCT CTGTGAGGAC CTCATCTGGA ACAGAGACCC TGAGGCCACC 
GAGAAGCTCT TACACTACGC CCAGACTCAG GGCAAAGGAG GGAAGAAAGT CATCCAGACT 
GATGAGTGGA GGAATGGCCC TCTCGAAGAA CGCCTTGAGT ACGCTCTTGT GAAGGGCATT 
GAAAAATATA TTATTGAAGA TACTGAGGAA GCCAGGTTAA ACCAGGAAAA ATATCCCCGA 
CCTCTGAATA TAATTGAAGG GCCGCTGATG AATGGCATGA AAATTGTTGG TGATCTTTTT 
GGAGCTGGAA AAATGTTTCT ACCTCAGGTT ATAAAGTCAG CTCGGGTCAT GAAGAAGGCC 
GTGGGTCACC TTATTCCCTT CATGGAAAAA GAGAGAGAAG AAACCAAAGT GCTTACTGGC 
AAAATAGAAG ATGAGGACCC TTACCAAGGC ACCATCGTGC TGGCTACTGT TAAAGGTGAC 
GTACACGACA TAGGCAAGAA CATAGTTGGA GTGGTTCTTG GCTGCAATAA TTTCAGAGTT 
ATTGATTTAG GAGTCATGAC TCCATGTGAC AAGATACTGA AGGCTGCTCT AGACCACAAA 
GCAGATATAA TTGGCCTGTC AGGACTCATC ACTCCTTCCC TGGATGAAAT GATTTTTGTC 
GCTAAGGAAA TGGAGAGACT AGCTATAAAG ATCCCATTGT TGATCGGAGG AGCTACCACT 
TCCAGAACCC ACACAGCAGT TAAAATAGCC CCAAGATACA GTGCGCCCGT GATCCACGTC 
CTGGATGCAT CCAAGAGCGT GGTGGTGTGT TCTCAACTGT TAGATGAGAA TCTAAAAGAT 
GAGTACTTTG AGGAGATCCT GGAAGAATAT GAAGATATTA GACAGGACCA TTATGAATCT 
CTCAAGGAGA GAAGATACTT AACCTTAAGG CAAGCTAGAG AAAATGGTTT CCATATTGAC 
TGGCTGTCAG AGCCTCCTCC AGTGAAGCCC ACGTTCCTCG GGACTCGGGT CTTTGAAGAC 
TACGACCTGC AGAAGCTGGT GGACTACATT GATTGGAAGC CTTTCTTCGA CGTCTGGCAG 
CTCCGGGGCA AATACCCGAA CCGAGGCTTT CCCAAGATAT TTGACGACAA AACTGTAGGT 
GAAGAGGCCA AAAAGGTCTA TGATGATGCC CAAAATATGC TGCAGGCACT GATCAGTCAG 
AAGAAGCTCC AGGCCAGGGG TGTGGTTGGG TTCTGGCCAG CACAGAGCAT TCAGGATGAT 
ATCCACCTGT ACGCGGAAGG CGCAGTGCCA CAGGCCTCGG AGCCCATAGC CACCTTCTAC 
GGGCTCAGGC AGCAGGCCGA GAAGGACTCT GCCAGCTCGG ACCCCTACCT CTGCCTCTCG 
GACTTCATCG CCCCTCTGCA CTCAGGTATC CCCGACTACC TGGGCCTGTT CGCCGTTGCC 
TGCTTTGGGG TGGAGGAGCT GAGCAAGGCC TACGAGGAGG AGTGTGATGA CTACAGCAGC 
ATCATGGTCA AGGCGCTGGG GGACCGGCTG GCCGAGGCCT TCGCTGAGGA GCTGCACGAG 
CGGGTCCGCA GGGAGCTGTG GGGTTACTGT AGTGGTGAGC AGCTGGCTGT GGCTGACCTG 
CGGAGACTGC GCTACGAGGG CATCCGGCCA GCGCCCGGCT ATCCCAGCCA GCCTGACCAC 
ACTGAGAAGC TGACCATGTG GAGGCTGGCG GACGTGGAGC AGCGCACAGG CATTAGGTTA 
ACAGAATCGC TGGCCATGGC ACCTGCTTCA GCAGTATCTG GCCTTTACTT CTCCAACTTG 
AAGTCCAAGT ATTTTGCCGT GGGGAAAATT TCCAAGGATC AGATTGAGGA TTATGCTTCG 
AGGAAGAACA TGTCTGTGGC CGAGGTTGAG AAATGGCTTG GACCCATTTT GGGATATGAT 
ACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR ( XM_024986503.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842271.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa231519
Clone ID Related Accession (Same CDS sequence)	XM_024986503.1
Accession Version	XM_024986503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methionine synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACCTT TTATTGAAAC AATTGGAAAA TGTACAACAG CTTATGTTCT CTGTTATCCC 
AATGCAGGTC TTCCCAATAC CTTCGGTGAC TATGATGAAA CTCCCCACGT GATGGCCATG 
CATTTAAAGG ATTTTGCTGT TGATGGCTTG GTCAACATAG TTGGCGGATG CTGTGGTACG 
ACACCAGATC ATATCAGAGA AATTGCTGAA GCTGTGAAAA ACTGTAAGCC TAGAGTTCCA 
CCTGCCACTG TTTTCGAAGG GCATATGCTA CTGTCTGGTC TAGAGCCCTT CAGGATTGGA 
CCATACACCA ACTTTGTTAA CATTGGAGAG CGCTGTAACG TTGCAGGATC CAGGAGATTT 
GCTAAGCTCA TCATGGCAGG AAATTATGAA GAAGCACTGA GCGTTGCCAA AATGCAGGTG 
GAAATGGGAG CCCAGGTGTT GGATATTAAC ATGGATGATG GCATGCTGGA TGGTCCAAGT 
GCAATGACCA GATTCTGCAA CTTCATTGCT TCCGAGCCTG ACATTGCCAA GGTGCCCTTG 
TGCATTGACT CTTCCAATTT TGCTGTGATT GAAGCCGGCT TAAAGTGCTG CCAGGGGAAG 
TGCATTGTTA ATAGCATCAG CTTGAAGGAG GGAGAGGATG ACTTCTTGGA GAAGGCCAGA 
AAGATTAAGA AGTTTGGAGC TGCCGTGGTG GTTATGGCTT TTGATGAAGA AGGACAGGCA 
ACAGAAACAG ATACCAAAAT CAGAGTGTGC ACCCGGGCCT ACCATCTCCT TTTGAAAAAA 
CTGGGCTTCA ATCCAAATGA CATCATCTTT GACCCTAATA TCCTCACCAT TGGTACTGGG 
ATGGAAGAAC ATAACTTGTA TGCTGTTAAT TTTATCAATG CAACAAAAGT CATTAAAGAA 
ACGCTGCCTG GAGCCAAAGT AAGCGGTGGT CTTTCCAACT TGTCCTTCTC CTTCCGAGGA 
ATGGAAGCCA TTCGAGAGGC AATGCATGGG GTTTTCCTGT ACCATGCGAT CAAGTTTGGT 
ATGGACATGG GAATAGTGAA TGCTGGAAGC CTTCCTGTAT ATGATGACAT CCACAAGGAG 
CTTCTCCAGC TCTGTGAGGA CCTCATCTGG AACAGAGACC CTGAGGCCAC CGAGAAGCTC 
TTACACTACG CCCAGACTCA GGGCAAAGGA GGGAAGAAAG TCATCCAGAC TGATGAGTGG 
AGGAATGGCC CTCTCGAAGA ACGCCTTGAG TACGCTCTTG TGAAGGGCAT TGAAAAATAT 
ATTATTGAAG ATACTGAGGA AGCCAGGTTA AACCAGGAAA AATATCCCCG ACCTCTGAAT 
ATAATTGAAG GGCCGCTGAT GAATGGCATG AAAATTGTTG GTGATCTTTT TGGAGCTGGA 
AAAATGTTTC TACCTCAGGT TATAAAGTCA GCTCGGGTCA TGAAGAAGGC CGTGGGTCAC 
CTTATTCCCT TCATGGAAAA AGAGAGAGAA GAAACCAAAG TGCTTACTGG CAAAATAGAA 
GATGAGGACC CTTACCAAGG CACCATCGTG CTGGCTACTG TTAAAGGTGA CGTACACGAC 
ATAGGCAAGA ACATAGTTGG AGTGGTTCTT GGCTGCAATA ATTTCAGAGT TATTGATTTA 
GGAGTCATGA CTCCATGTGA CAAGATACTG AAGGCTGCTC TAGACCACAA AGCAGATATA 
ATTGGCCTGT CAGGACTCAT CACTCCTTCC CTGGATGAAA TGATTTTTGT CGCTAAGGAA 
ATGGAGAGAC TAGCTATAAA GATCCCATTG TTGATCGGAG GAGCTACCAC TTCCAGAACC 
CACACAGCAG TTAAAATAGC CCCAAGATAC AGTGCGCCCG TGATCCACGT CCTGGATGCA 
TCCAAGAGCG TGGTGGTGTG TTCTCAACTG TTAGATGAGA ATCTAAAAGA TGAGTACTTT 
GAGGAGATCC TGGAAGAATA TGAAGATATT AGACAGGACC ATTATGAATC TCTCAAGGAG 
AGAAGATACT TAACCTTAAG GCAAGCTAGA GAAAATGGTT TCCATATTGA CTGGCTGTCA 
GAGCCTCCTC CAGTGAAGCC CACGTTCCTC GGGACTCGGG TCTTTGAAGA CTACGACCTG 
CAGAAGCTGG TGGACTACAT TGATTGGAAG CCTTTCTTCG ACGTCTGGCA GCTCCGGGGC 
AAATACCCGA ACCGAGGCTT TCCCAAGATA TTTGACGACA AAACTGTAGG TGAAGAGGCC 
AAAAAGGTCT ATGATGATGC CCAAAATATG CTGCAGGCAC TGATCAGTCA GAAGAAGCTC 
CAGGCCAGGG GTGTGGTTGG GTTCTGGCCA GCACAGAGCA TTCAGGATGA TATCCACCTG 
TACGCGGAAG GCGCAGTGCC ACAGGCCTCG GAGCCCATAG CCACCTTCTA CGGGCTCAGG 
CAGCAGGCCG AGAAGGACTC TGCCAGCTCG GACCCCTACC TCTGCCTCTC GGACTTCATC 
GCCCCTCTGC ACTCAGGTAT CCCCGACTAC CTGGGCCTGT TCGCCGTTGC CTGCTTTGGG 
GTGGAGGAGC TGAGCAAGGC CTACGAGGAG GAGTGTGATG ACTACAGCAG CATCATGGTC 
AAGGCGCTGG GGGACCGGCT GGCCGAGGCC TTCGCTGAGG AGCTGCACGA GCGGGTCCGC 
AGGGAGCTGT GGGGTTACTG TAGTGGTGAG CAGCTGGCTG TGGCTGACCT GCGGAGACTG 
CGCTACGAGG GCATCCGGCC AGCGCCCGGC TATCCCAGCC AGCCTGACCA CACTGAGAAG 
CTGACCATGT GGAGGCTGGC GGACGTGGAG CAGCGCACAG GCATTAGGTT AACAGAATCG 
CTGGCCATGG CACCTGCTTC AGCAGTATCT GGCCTTTACT TCTCCAACTT GAAGTCCAAG 
TATTTTGCCG TGGGGAAAAT TTCCAAGGAT CAGATTGAGG ATTATGCTTC GAGGAAGAAC 
ATGTCTGTGG CCGAGGTTGA GAAATGGCTT GGACCCATTT TGGGATATGA TACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842271.1
CDS	122..3121
Translation	MRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPHVMAMHLKDFAVDGLVNIVGGCCGTTPDHIREIAEAVKNCKPRVPPATVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRRFAKLIMAGNYEEALSVAKMQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDTKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_024986503.1
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986503.1

ORF Insert Sequence:

ATGAGACCTT TTATTGAAAC AATTGGAAAA TGTACAACAG CTTATGTTCT CTGTTATCCC 
AATGCAGGTC TTCCCAATAC CTTCGGTGAC TATGATGAAA CTCCCCACGT GATGGCCATG 
CATTTAAAGG ATTTTGCTGT TGATGGCTTG GTCAACATAG TTGGCGGATG CTGTGGTACG 
ACACCAGATC ATATCAGAGA AATTGCTGAA GCTGTGAAAA ACTGTAAGCC TAGAGTTCCA 
CCTGCCACTG TTTTCGAAGG GCATATGCTA CTGTCTGGTC TAGAGCCCTT CAGGATTGGA 
CCATACACCA ACTTTGTTAA CATTGGAGAG CGCTGTAACG TTGCAGGATC CAGGAGATTT 
GCTAAGCTCA TCATGGCAGG AAATTATGAA GAAGCACTGA GCGTTGCCAA AATGCAGGTG 
GAAATGGGAG CCCAGGTGTT GGATATTAAC ATGGATGATG GCATGCTGGA TGGTCCAAGT 
GCAATGACCA GATTCTGCAA CTTCATTGCT TCCGAGCCTG ACATTGCCAA GGTGCCCTTG 
TGCATTGACT CTTCCAATTT TGCTGTGATT GAAGCCGGCT TAAAGTGCTG CCAGGGGAAG 
TGCATTGTTA ATAGCATCAG CTTGAAGGAG GGAGAGGATG ACTTCTTGGA GAAGGCCAGA 
AAGATTAAGA AGTTTGGAGC TGCCGTGGTG GTTATGGCTT TTGATGAAGA AGGACAGGCA 
ACAGAAACAG ATACCAAAAT CAGAGTGTGC ACCCGGGCCT ACCATCTCCT TTTGAAAAAA 
CTGGGCTTCA ATCCAAATGA CATCATCTTT GACCCTAATA TCCTCACCAT TGGTACTGGG 
ATGGAAGAAC ATAACTTGTA TGCTGTTAAT TTTATCAATG CAACAAAAGT CATTAAAGAA 
ACGCTGCCTG GAGCCAAAGT AAGCGGTGGT CTTTCCAACT TGTCCTTCTC CTTCCGAGGA 
ATGGAAGCCA TTCGAGAGGC AATGCATGGG GTTTTCCTGT ACCATGCGAT CAAGTTTGGT 
ATGGACATGG GAATAGTGAA TGCTGGAAGC CTTCCTGTAT ATGATGACAT CCACAAGGAG 
CTTCTCCAGC TCTGTGAGGA CCTCATCTGG AACAGAGACC CTGAGGCCAC CGAGAAGCTC 
TTACACTACG CCCAGACTCA GGGCAAAGGA GGGAAGAAAG TCATCCAGAC TGATGAGTGG 
AGGAATGGCC CTCTCGAAGA ACGCCTTGAG TACGCTCTTG TGAAGGGCAT TGAAAAATAT 
ATTATTGAAG ATACTGAGGA AGCCAGGTTA AACCAGGAAA AATATCCCCG ACCTCTGAAT 
ATAATTGAAG GGCCGCTGAT GAATGGCATG AAAATTGTTG GTGATCTTTT TGGAGCTGGA 
AAAATGTTTC TACCTCAGGT TATAAAGTCA GCTCGGGTCA TGAAGAAGGC CGTGGGTCAC 
CTTATTCCCT TCATGGAAAA AGAGAGAGAA GAAACCAAAG TGCTTACTGG CAAAATAGAA 
GATGAGGACC CTTACCAAGG CACCATCGTG CTGGCTACTG TTAAAGGTGA CGTACACGAC 
ATAGGCAAGA ACATAGTTGG AGTGGTTCTT GGCTGCAATA ATTTCAGAGT TATTGATTTA 
GGAGTCATGA CTCCATGTGA CAAGATACTG AAGGCTGCTC TAGACCACAA AGCAGATATA 
ATTGGCCTGT CAGGACTCAT CACTCCTTCC CTGGATGAAA TGATTTTTGT CGCTAAGGAA 
ATGGAGAGAC TAGCTATAAA GATCCCATTG TTGATCGGAG GAGCTACCAC TTCCAGAACC 
CACACAGCAG TTAAAATAGC CCCAAGATAC AGTGCGCCCG TGATCCACGT CCTGGATGCA 
TCCAAGAGCG TGGTGGTGTG TTCTCAACTG TTAGATGAGA ATCTAAAAGA TGAGTACTTT 
GAGGAGATCC TGGAAGAATA TGAAGATATT AGACAGGACC ATTATGAATC TCTCAAGGAG 
AGAAGATACT TAACCTTAAG GCAAGCTAGA GAAAATGGTT TCCATATTGA CTGGCTGTCA 
GAGCCTCCTC CAGTGAAGCC CACGTTCCTC GGGACTCGGG TCTTTGAAGA CTACGACCTG 
CAGAAGCTGG TGGACTACAT TGATTGGAAG CCTTTCTTCG ACGTCTGGCA GCTCCGGGGC 
AAATACCCGA ACCGAGGCTT TCCCAAGATA TTTGACGACA AAACTGTAGG TGAAGAGGCC 
AAAAAGGTCT ATGATGATGC CCAAAATATG CTGCAGGCAC TGATCAGTCA GAAGAAGCTC 
CAGGCCAGGG GTGTGGTTGG GTTCTGGCCA GCACAGAGCA TTCAGGATGA TATCCACCTG 
TACGCGGAAG GCGCAGTGCC ACAGGCCTCG GAGCCCATAG CCACCTTCTA CGGGCTCAGG 
CAGCAGGCCG AGAAGGACTC TGCCAGCTCG GACCCCTACC TCTGCCTCTC GGACTTCATC 
GCCCCTCTGC ACTCAGGTAT CCCCGACTAC CTGGGCCTGT TCGCCGTTGC CTGCTTTGGG 
GTGGAGGAGC TGAGCAAGGC CTACGAGGAG GAGTGTGATG ACTACAGCAG CATCATGGTC 
AAGGCGCTGG GGGACCGGCT GGCCGAGGCC TTCGCTGAGG AGCTGCACGA GCGGGTCCGC 
AGGGAGCTGT GGGGTTACTG TAGTGGTGAG CAGCTGGCTG TGGCTGACCT GCGGAGACTG 
CGCTACGAGG GCATCCGGCC AGCGCCCGGC TATCCCAGCC AGCCTGACCA CACTGAGAAG 
CTGACCATGT GGAGGCTGGC GGACGTGGAG CAGCGCACAG GCATTAGGTT AACAGAATCG 
CTGGCCATGG CACCTGCTTC AGCAGTATCT GGCCTTTACT TCTCCAACTT GAAGTCCAAG 
TATTTTGCCG TGGGGAAAAT TTCCAAGGAT CAGATTGAGG ATTATGCTTC GAGGAAGAAC 
ATGTCTGTGG CCGAGGTTGA GAAATGGCTT GGACCCATTT TGGGATATGA TACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR ( XM_024986502.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842270.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45030
Clone ID Related Accession (Same CDS sequence)	XM_024986502.1
Accession Version	XM_024986502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methionine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGA CCCTGCAAGA TGAGATTGAA GCTATCCTGC AGGAGAGGAT TATGGTGCTT 
GATGGAGGGA TGGGGACCAT GATCCAGCAG CACAAGCTGA GTGAAGAAGA CTTCCGAGGA 
CAAGAATTTA AAGATCATGC CAGGCCCCTG AAAGGCAACA ATGACATTTT AAGTATAACT 
CAGCCCAGTG TCATTTACCA AATCCATAAG GAGTACCTCC TAGCTGGGGC AGATATCATT 
GAAACAAATA CTTTCAGCAG CACTTCTATT GCCCAAGCTG ACTATGGCCT GGAACACTTG 
GCCTACCGGA TGAACATGTG CTCTGCGGGA GTGGCCAGAA AAGCAGCCGA GGACATCTCT 
CTCCAGACAG GAATTAAGAG GTATGTGGCA GGAGCTCTGG GTCCAACTAA CAAGACACTC 
TCTGTGTCGC CATCTGTGGA AAGACCAGAT TACAGGAACA TCACATTTGA TGAACTTGTT 
GAAGCATACA AAGAGCAGGC CAAAGGGCTT CTGGATGGTG GAGTGGATAT CTTACTCATT 
GAAACTATTT TTGATACCGC CAATGCCAAG GCAGCCTTGT TTGCAGTCCA AAAACTTTTT 
GAAGAGGAGT ATGTTCCCCG GCCTGTCTTT ATTTCAGGGA CAATTGTTGA TAAAAGTGGG 
CGGACTCTCT CTGGCCAGAC TGGGGAGGCA TTTGTCATCA GCGTGTCTCA TGCAGACCCA 
CTCTGCATTG GATTAAATTG TGCTCTGGGT GCAGCTGAAA TGAGACCTTT TATTGAAACA 
ATTGGAAAAT GTACAACAGC TTATGTTCTC TGTTATCCCA ATGCAGGTCT TCCCAATACC 
TTCGGTGACT ATGATGAAAC TCCCCACGTG ATGGCCATGC ATTTAAAGGA TTTTGCTGTT 
GATGGCTTGG TCAACATAGT TGGCGGATGC TGTGGTACGA CACCAGATCA TATCAGAGAA 
ATTGCTGAAG CTGTGAAAAA CTGTAAGCCT AGAGTTCCAC CTGCCACTGT TTTCGAAGGG 
CATATGCTAC TGTCTGGTCT AGAGCCCTTC AGGATTGGAC CATACACCAA CTTTGTTAAC 
ATTGGAGAGC GCTGTAACGT TGCAGGATCC AGGAGATTTG CTAAGCTCAT CATGGCAGGA 
AATTATGAAG AAGCACTGAG CGTTGCCAAA ATGCAGGTGG AAATGGGAGC CCAGGTGTTG 
GATATTAACA TGGATGATGG CATGCTGGAT GGTCCAAGTG CAATGACCAG ATTCTGCAAC 
TTCATTGCTT CCGAGCCTGA CATTGCCAAG GTGCCCTTGT GCATTGACTC TTCCAATTTT 
GCTGTGATTG AAGCCGGCTT AAAGTGCTGC CAGGGGAAGT GCATTGTTAA TAGCATCAGC 
TTGAAGGAGG GAGAGGATGA CTTCTTGGAG AAGGCCAGAA AGATTAAGAA GTTTGGAGCT 
GCCGTGGTGG TTATGGCTTT TGATGAAGAA GGACAGGCAA CAGAAACAGA TACCAAAATC 
AGAGTGTGCA CCCGGGCCTA CCATCTCCTT TTGAAAAAAC TGGGCTTCAA TCCAAATGAC 
ATCATCTTTG ACCCTAATAT CCTCACCATT GGTACTGGGA TGGAAGAACA TAACTTGTAT 
GCTGTTAATT TTATCAATGC AACAAAAGTC ATTAAAGAAA CGCTGCCTGG AGCCAAAGTA 
AGCGGTGGTC TTTCCAACTT GTCCTTCTCC TTCCGAGGAA TGGAAGCCAT TCGAGAGGCA 
ATGCATGGGG TTTTCCTGTA CCATGCGATC AAGTTTGGTA TGGACATGGG AATAGTGAAT 
GCTGGAAGCC TTCCTGTATA TGATGACATC CACAAGGAGC TTCTCCAGCT CTGTGAGGAC 
CTCATCTGGA ACAGAGACCC TGAGGCCACC GAGAAGCTCT TACACTACGC CCAGACTCAG 
GGCAAAGGAG GGAAGAAAGT CATCCAGACT GATGAGTGGA GGAATGGCCC TCTCGAAGAA 
CGCCTTGAGT ACGCTCTTGT GAAGGGCATT GAAAAATATA TTATTGAAGA TACTGAGGAA 
GCCAGGTTAA ACCAGGAAAA ATATCCCCGA CCTCTGAATA TAATTGAAGG GCCGCTGATG 
AATGGCATGA AAATTGTTGG TGATCTTTTT GGAGCTGGAA AAATGTTTCT ACCTCAGGTT 
ATAAAGTCAG CTCGGGTCAT GAAGAAGGCC GTGGGTCACC TTATTCCCTT CATGGAAAAA 
GAGAGAGAAG AAACCAAAGT GCTTACTGGC AAAATAGAAG ATGAGGACCC TTACCAAGGC 
ACCATCGTGC TGGCTACTGT TAAAGGTGAC GTACACGACA TAGGCAAGAA CATAGTTGGA 
GTGGTTCTTG GCTGCAATAA TTTCAGAGTT ATTGATTTAG GAGTCATGAC TCCATGTGAC 
AAGATACTGA AGGCTGCTCT AGACCACAAA GCAGATATAA TTGGCCTGTC AGGACTCATC 
ACTCCTTCCC TGGATGAAAT GATTTTTGTC GCTAAGGAAA TGGAGAGACT AGCTATAAAG 
ATCCCATTGT TGATCGGAGG AGCTACCACT TCCAGAACCC ACACAGCAGT TAAAATAGCC 
CCAAGATACA GTGCGCCCGT GATCCACGTC CTGGATGCAT CCAAGAGCGT GGTGGTGTGT 
TCTCAACTGT TAGATGAGAA TCTAAAAGAT GAGTACTTTG AGGAGATCCT GGAAGAATAT 
GAAGATATTA GACAGGACCA TTATGAATCT CTCAAGGAGA GAAGATACTT AACCTTAAGG 
CAAGCTAGAG AAAATGGTTT CCATATTGAC TGGCTGTCAG AGCCTCCTCC AGTGAAGCCC 
ACGTTCCTCG GGACTCGGGT CTTTGAAGAC TACGACCTGC AGAAGCTGGT GGACTACATT 
GATTGGAAGC CTTTCTTCGA CGTCTGGCAG CTCCGGGGCA AATACCCGAA CCGAGGCTTT 
CCCAAGATAT TTGACGACAA AACTGTAGGT GAAGAGGCCA AAAAGGTCTA TGATGATGCC 
CAAAATATGC TGCAGGCACT GATCAGTCAG AAGAAGCTCC AGGCCAGGGG TGTGGTTGGG 
TTCTGGCCAG CACAGAGCAT TCAGGATGAT ATCCACCTGT ACGCGGAAGG CGCAGTGCCA 
CAGGCCTCGG AGCCCATAGC CACCTTCTAC GGGCTCAGGC AGCAGGCCGA GAAGGACTCT 
GCCAGCTCGG ACCCCTACCT CTGCCTCTCG GACTTCATCG CCCCTCTGCA CTCAGGTATC 
CCCGACTACC TGGGCCTGTT CGCCGTTGCC TGCTTTGGGG TGGAGGAGCT GAGCAAGGCC 
TACGAGGAGG AGTGTGATGA CTACAGCAGC ATCATGGTCA AGGCGCTGGG GGACCGGCTG 
GCCGAGGCCT TCGCTGAGGA GCTGCACGAG CGGGTCCGCA GGGAGCTGTG GGGTTACTGT 
AGTGGTGAGC AGCTGGCTGT GGCTGACCTG CGGAGACTGC GCTACGAGGG CATCCGGCCA 
GCGCCCGGCT ATCCCAGCCA GCCTGACCAC ACTGAGAAGC TGACCATGTG GAGGCTGGCG 
GACGTGGAGC AGCGCACAGG CATTAGGTTA ACAGAATCGC TGGCCATGGC ACCTGCTTCA 
GCAGTATCTG GCCTTTACTT CTCCAACTTG AAGTCCAAGT ATTTTGCCGT GGGGAAAATT 
TCCAAGGATC AGATTGAGGA TTATGCTTCG AGGAAGAACA TGTCTGTGGC CGAGGTTGAG 
AAATGGCTTG GACCCATTTT GGGATATGAT ACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842270.1
CDS	161..3919
Translation	MKKTLQDEIEAILQERIMVLDGGMGTMIQQHKLSEEDFRGQEFKDHARPLKGNNDILSITQPSVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEDISLQTGIKRYVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYKEQAKGLLDGGVDILLIETIFDTANAKAALFAVQKLFEEEYVPRPVFISGTIVDKSGRTLSGQTGEAFVISVSHADPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPHVMAMHLKDFAVDGLVNIVGGCCGTTPDHIREIAEAVKNCKPRVPPATVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRRFAKLIMAGNYEEALSVAKMQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDTKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_024986502.1
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986502.1

ORF Insert Sequence:

ATGAAGAAGA CCCTGCAAGA TGAGATTGAA GCTATCCTGC AGGAGAGGAT TATGGTGCTT 
GATGGAGGGA TGGGGACCAT GATCCAGCAG CACAAGCTGA GTGAAGAAGA CTTCCGAGGA 
CAAGAATTTA AAGATCATGC CAGGCCCCTG AAAGGCAACA ATGACATTTT AAGTATAACT 
CAGCCCAGTG TCATTTACCA AATCCATAAG GAGTACCTCC TAGCTGGGGC AGATATCATT 
GAAACAAATA CTTTCAGCAG CACTTCTATT GCCCAAGCTG ACTATGGCCT GGAACACTTG 
GCCTACCGGA TGAACATGTG CTCTGCGGGA GTGGCCAGAA AAGCAGCCGA GGACATCTCT 
CTCCAGACAG GAATTAAGAG GTATGTGGCA GGAGCTCTGG GTCCAACTAA CAAGACACTC 
TCTGTGTCGC CATCTGTGGA AAGACCAGAT TACAGGAACA TCACATTTGA TGAACTTGTT 
GAAGCATACA AAGAGCAGGC CAAAGGGCTT CTGGATGGTG GAGTGGATAT CTTACTCATT 
GAAACTATTT TTGATACCGC CAATGCCAAG GCAGCCTTGT TTGCAGTCCA AAAACTTTTT 
GAAGAGGAGT ATGTTCCCCG GCCTGTCTTT ATTTCAGGGA CAATTGTTGA TAAAAGTGGG 
CGGACTCTCT CTGGCCAGAC TGGGGAGGCA TTTGTCATCA GCGTGTCTCA TGCAGACCCA 
CTCTGCATTG GATTAAATTG TGCTCTGGGT GCAGCTGAAA TGAGACCTTT TATTGAAACA 
ATTGGAAAAT GTACAACAGC TTATGTTCTC TGTTATCCCA ATGCAGGTCT TCCCAATACC 
TTCGGTGACT ATGATGAAAC TCCCCACGTG ATGGCCATGC ATTTAAAGGA TTTTGCTGTT 
GATGGCTTGG TCAACATAGT TGGCGGATGC TGTGGTACGA CACCAGATCA TATCAGAGAA 
ATTGCTGAAG CTGTGAAAAA CTGTAAGCCT AGAGTTCCAC CTGCCACTGT TTTCGAAGGG 
CATATGCTAC TGTCTGGTCT AGAGCCCTTC AGGATTGGAC CATACACCAA CTTTGTTAAC 
ATTGGAGAGC GCTGTAACGT TGCAGGATCC AGGAGATTTG CTAAGCTCAT CATGGCAGGA 
AATTATGAAG AAGCACTGAG CGTTGCCAAA ATGCAGGTGG AAATGGGAGC CCAGGTGTTG 
GATATTAACA TGGATGATGG CATGCTGGAT GGTCCAAGTG CAATGACCAG ATTCTGCAAC 
TTCATTGCTT CCGAGCCTGA CATTGCCAAG GTGCCCTTGT GCATTGACTC TTCCAATTTT 
GCTGTGATTG AAGCCGGCTT AAAGTGCTGC CAGGGGAAGT GCATTGTTAA TAGCATCAGC 
TTGAAGGAGG GAGAGGATGA CTTCTTGGAG AAGGCCAGAA AGATTAAGAA GTTTGGAGCT 
GCCGTGGTGG TTATGGCTTT TGATGAAGAA GGACAGGCAA CAGAAACAGA TACCAAAATC 
AGAGTGTGCA CCCGGGCCTA CCATCTCCTT TTGAAAAAAC TGGGCTTCAA TCCAAATGAC 
ATCATCTTTG ACCCTAATAT CCTCACCATT GGTACTGGGA TGGAAGAACA TAACTTGTAT 
GCTGTTAATT TTATCAATGC AACAAAAGTC ATTAAAGAAA CGCTGCCTGG AGCCAAAGTA 
AGCGGTGGTC TTTCCAACTT GTCCTTCTCC TTCCGAGGAA TGGAAGCCAT TCGAGAGGCA 
ATGCATGGGG TTTTCCTGTA CCATGCGATC AAGTTTGGTA TGGACATGGG AATAGTGAAT 
GCTGGAAGCC TTCCTGTATA TGATGACATC CACAAGGAGC TTCTCCAGCT CTGTGAGGAC 
CTCATCTGGA ACAGAGACCC TGAGGCCACC GAGAAGCTCT TACACTACGC CCAGACTCAG 
GGCAAAGGAG GGAAGAAAGT CATCCAGACT GATGAGTGGA GGAATGGCCC TCTCGAAGAA 
CGCCTTGAGT ACGCTCTTGT GAAGGGCATT GAAAAATATA TTATTGAAGA TACTGAGGAA 
GCCAGGTTAA ACCAGGAAAA ATATCCCCGA CCTCTGAATA TAATTGAAGG GCCGCTGATG 
AATGGCATGA AAATTGTTGG TGATCTTTTT GGAGCTGGAA AAATGTTTCT ACCTCAGGTT 
ATAAAGTCAG CTCGGGTCAT GAAGAAGGCC GTGGGTCACC TTATTCCCTT CATGGAAAAA 
GAGAGAGAAG AAACCAAAGT GCTTACTGGC AAAATAGAAG ATGAGGACCC TTACCAAGGC 
ACCATCGTGC TGGCTACTGT TAAAGGTGAC GTACACGACA TAGGCAAGAA CATAGTTGGA 
GTGGTTCTTG GCTGCAATAA TTTCAGAGTT ATTGATTTAG GAGTCATGAC TCCATGTGAC 
AAGATACTGA AGGCTGCTCT AGACCACAAA GCAGATATAA TTGGCCTGTC AGGACTCATC 
ACTCCTTCCC TGGATGAAAT GATTTTTGTC GCTAAGGAAA TGGAGAGACT AGCTATAAAG 
ATCCCATTGT TGATCGGAGG AGCTACCACT TCCAGAACCC ACACAGCAGT TAAAATAGCC 
CCAAGATACA GTGCGCCCGT GATCCACGTC CTGGATGCAT CCAAGAGCGT GGTGGTGTGT 
TCTCAACTGT TAGATGAGAA TCTAAAAGAT GAGTACTTTG AGGAGATCCT GGAAGAATAT 
GAAGATATTA GACAGGACCA TTATGAATCT CTCAAGGAGA GAAGATACTT AACCTTAAGG 
CAAGCTAGAG AAAATGGTTT CCATATTGAC TGGCTGTCAG AGCCTCCTCC AGTGAAGCCC 
ACGTTCCTCG GGACTCGGGT CTTTGAAGAC TACGACCTGC AGAAGCTGGT GGACTACATT 
GATTGGAAGC CTTTCTTCGA CGTCTGGCAG CTCCGGGGCA AATACCCGAA CCGAGGCTTT 
CCCAAGATAT TTGACGACAA AACTGTAGGT GAAGAGGCCA AAAAGGTCTA TGATGATGCC 
CAAAATATGC TGCAGGCACT GATCAGTCAG AAGAAGCTCC AGGCCAGGGG TGTGGTTGGG 
TTCTGGCCAG CACAGAGCAT TCAGGATGAT ATCCACCTGT ACGCGGAAGG CGCAGTGCCA 
CAGGCCTCGG AGCCCATAGC CACCTTCTAC GGGCTCAGGC AGCAGGCCGA GAAGGACTCT 
GCCAGCTCGG ACCCCTACCT CTGCCTCTCG GACTTCATCG CCCCTCTGCA CTCAGGTATC 
CCCGACTACC TGGGCCTGTT CGCCGTTGCC TGCTTTGGGG TGGAGGAGCT GAGCAAGGCC 
TACGAGGAGG AGTGTGATGA CTACAGCAGC ATCATGGTCA AGGCGCTGGG GGACCGGCTG 
GCCGAGGCCT TCGCTGAGGA GCTGCACGAG CGGGTCCGCA GGGAGCTGTG GGGTTACTGT 
AGTGGTGAGC AGCTGGCTGT GGCTGACCTG CGGAGACTGC GCTACGAGGG CATCCGGCCA 
GCGCCCGGCT ATCCCAGCCA GCCTGACCAC ACTGAGAAGC TGACCATGTG GAGGCTGGCG 
GACGTGGAGC AGCGCACAGG CATTAGGTTA ACAGAATCGC TGGCCATGGC ACCTGCTTCA 
GCAGTATCTG GCCTTTACTT CTCCAACTTG AAGTCCAAGT ATTTTGCCGT GGGGAAAATT 
TCCAAGGATC AGATTGAGGA TTATGCTTCG AGGAAGAACA TGTCTGTGGC CGAGGTTGAG 
AAATGGCTTG GACCCATTTT GGGATATGAT ACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR ( XM_015460920.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316406.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.

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CloneID	OBa88869
Clone ID Related Accession (Same CDS sequence)	XM_015460920.1
Accession Version	XM_015460920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methionine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104619.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGT GCTCTGCGGG AGTGGCCAGA AAAGCAGCCG AGGACATCTC TCTCCAGACA 
GGAATTAAGA GGTATGTGGC AGGAGCTCTG GGTCCAACTA ACAAGACACT CTCTGTGTCG 
CCATCTGTGG AAAGACCAGA TTACAGGAAC ATCACATTTG ATGAACTTGT TGAAGCATAC 
AAAGAGCAGG CCAAAGGGCT TCTGGATGGT GGAGTGGATA TCTTACTCAT TGAAACTATT 
TTTGATACCG CCAATGCCAA GGCAGCCTTG TTTGCAGTCC AAAAACTTTT TGAAGAGGAG 
TATGTTCCCC GGCCTGTCTT TATTTCAGGG ACAATTGTTG ATAAAAGTGG GCGGACTCTC 
TCTGGCCAGA CTGGGGAGGC ATTTGTCATC AGCGTGTCTC ATGCAGACCC ACTCTGCATT 
GGATTAAATT GTGCTCTGGG TGCAGCTGAA ATGAGACCTT TTATTGAAAC AATTGGAAAA 
TGTACAACAG CTTATGTTCT CTGTTATCCC AATGCAGGTC TTCCCAATAC CTTCGGTGAC 
TATGATGAAA CTCCCCACGT GATGGCCATG CATTTAAAGG ATTTTGCTGT TGATGGCTTG 
GTCAACATAG TTGGCGGATG CTGTGGTACG ACACCAGATC ATATCAGAGA AATTGCTGAA 
GCTGTGAAAA ACTGTAAGCC TAGAGTTCCA CCTGCCACTG TTTTCGAAGG GCATATGCTA 
CTGTCTGGTC TAGAGCCCTT CAGGATTGGA CCATACACCA ACTTTGTTAA CATTGGAGAG 
CGCTGTAACG TTGCAGGATC CAGGAGATTT GCTAAGCTCA TCATGGCAGG AAATTATGAA 
GAAGCACTGA GCGTTGCCAA AATGCAGGTG GAAATGGGAG CCCAGGTGTT GGATATTAAC 
ATGGATGATG GCATGCTGGA TGGTCCAAGT GCAATGACCA GATTCTGCAA CTTCATTGCT 
TCCGAGCCTG ACATTGCCAA GGTGCCCTTG TGCATTGACT CTTCCAATTT TGCTGTGATT 
GAAGCCGGCT TAAAGTGCTG CCAGGGGAAG TGCATTGTTA ATAGCATCAG CTTGAAGGAG 
GGAGAGGATG ACTTCTTGGA GAAGGCCAGA AAGATTAAGA AGTTTGGAGC TGCCGTGGTG 
GTTATGGCTT TTGATGAAGA AGGACAGGCA ACAGAAACAG ATACCAAAAT CAGAGTGTGC 
ACCCGGGCCT ACCATCTCCT TTTGAAAAAA CTGGGCTTCA ATCCAAATGA CATCATCTTT 
GACCCTAATA TCCTCACCAT TGGTACTGGG ATGGAAGAAC ATAACTTGTA TGCTGTTAAT 
TTTATCAATG CAACAAAAGT CATTAAAGAA ACGCTGCCTG GAGCCAAAGT AAGCGGTGGT 
CTTTCCAACT TGTCCTTCTC CTTCCGAGGA ATGGAAGCCA TTCGAGAGGC AATGCATGGG 
GTTTTCCTGT ACCATGCGAT CAAGTTTGGT ATGGACATGG GAATAGTGAA TGCTGGAAGC 
CTTCCTGTAT ATGATGACAT CCACAAGGAG CTTCTCCAGC TCTGTGAGGA CCTCATCTGG 
AACAGAGACC CTGAGGCCAC CGAGAAGCTC TTACACTACG CCCAGACTCA GGGCAAAGGA 
GGGAAGAAAG TCATCCAGAC TGATGAGTGG AGGAATGGCC CTCTCGAAGA ACGCCTTGAG 
TACGCTCTTG TGAAGGGCAT TGAAAAATAT ATTATTGAAG ATACTGAGGA AGCCAGGTTA 
AACCAGGAAA AATATCCCCG ACCTCTGAAT ATAATTGAAG GGCCGCTGAT GAATGGCATG 
AAAATTGTTG GTGATCTTTT TGGAGCTGGA AAAATGTTTC TACCTCAGGT TATAAAGTCA 
GCTCGGGTCA TGAAGAAGGC CGTGGGTCAC CTTATTCCCT TCATGGAAAA AGAGAGAGAA 
GAAACCAAAG TGCTTACTGG CAAAATAGAA GATGAGGACC CTTACCAAGG CACCATCGTG 
CTGGCTACTG TTAAAGGTGA CGTACACGAC ATAGGCAAGA ACATAGTTGG AGTGGTTCTT 
GGCTGCAATA ATTTCAGAGT TATTGATTTA GGAGTCATGA CTCCATGTGA CAAGATACTG 
AAGGCTGCTC TAGACCACAA AGCAGATATA ATTGGCCTGT CAGGACTCAT CACTCCTTCC 
CTGGATGAAA TGATTTTTGT CGCTAAGGAA ATGGAGAGAC TAGCTATAAA GATCCCATTG 
TTGATCGGAG GAGCTACCAC TTCCAGAACC CACACAGCAG TTAAAATAGC CCCAAGATAC 
AGTGCGCCCG TGATCCACGT CCTGGATGCA TCCAAGAGCG TGGTGGTGTG TTCTCAACTG 
TTAGATGAGA ATCTAAAAGA TGAGTACTTT GAGGAGATCC TGGAAGAATA TGAAGATATT 
AGACAGGACC ATTATGAATC TCTCAAGGAG AGAAGATACT TAACCTTAAG GCAAGCTAGA 
GAAAATGGTT TCCATATTGA CTGGCTGTCA GAGCCTCCTC CAGTGAAGCC CACGTTCCTC 
GGGACTCGGG TCTTTGAAGA CTACGACCTG CAGAAGCTGG TGGACTACAT TGATTGGAAG 
CCTTTCTTCG ACGTCTGGCA GCTCCGGGGC AAATACCCGA ACCGAGGCTT TCCCAAGATA 
TTTGACGACA AAACTGTAGG TGAAGAGGCC AAAAAGGTCT ATGATGATGC CCAAAATATG 
CTGCAGGCAC TGATCAGTCA GAAGAAGCTC CAGGCCAGGG GTGTGGTTGG GTTCTGGCCA 
GCACAGAGCA TTCAGGATGA TATCCACCTG TACGCGGAAG GCGCAGTGCC ACAGGCCTCG 
GAGCCCATAG CCACCTTCTA CGGGCTCAGG CAGCAGGCCG AGAAGGACTC TGCCAGCTCG 
GACCCCTACC TCTGCCTCTC GGACTTCATC GCCCCTCTGC ACTCAGGTAT CCCCGACTAC 
CTGGGCCTGT TCGCCGTTGC CTGCTTTGGG GTGGAGGAGC TGAGCAAGGC CTACGAGGAG 
GAGTGTGATG ACTACAGCAG CATCATGGTC AAGGCGCTGG GGGACCGGCT GGCCGAGGCC 
TTCGCTGAGG AGCTGCACGA GCGGGTCCGC AGGGAGCTGT GGGGTTACTG TAGTGGTGAG 
CAGCTGGCTG TGGCTGACCT GCGGAGACTG CGCTACGAGG GCATCCGGCC AGCGCCCGGC 
TATCCCAGCC AGCCTGACCA CACTGAGAAG CTGACCATGT GGAGGCTGGC GGACGTGGAG 
CAGCGCACAG GCATTAGGTT AACAGAATCG CTGGCCATGG CACCTGCTTC AGCAGTATCT 
GGCCTTTACT TCTCCAACTT GAAGTCCAAG TATTTTGCCG TGGGGAAAAT TTCCAAGGAT 
CAGATTGAGG ATTATGCTTC GAGGAAGAAC ATGTCTGTGG CCGAGGTTGA GAAATGGCTT 
GGACCCATTT TGGGATATGA TACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316406.1
CDS	154..3603
Translation	MNMCSAGVARKAAEDISLQTGIKRYVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYKEQAKGLLDGGVDILLIETIFDTANAKAALFAVQKLFEEEYVPRPVFISGTIVDKSGRTLSGQTGEAFVISVSHADPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPHVMAMHLKDFAVDGLVNIVGGCCGTTPDHIREIAEAVKNCKPRVPPATVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRRFAKLIMAGNYEEALSVAKMQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDTKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_015460920.1
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460920.1

ORF Insert Sequence:

ATGAACATGT GCTCTGCGGG AGTGGCCAGA AAAGCAGCCG AGGACATCTC TCTCCAGACA 
GGAATTAAGA GGTATGTGGC AGGAGCTCTG GGTCCAACTA ACAAGACACT CTCTGTGTCG 
CCATCTGTGG AAAGACCAGA TTACAGGAAC ATCACATTTG ATGAACTTGT TGAAGCATAC 
AAAGAGCAGG CCAAAGGGCT TCTGGATGGT GGAGTGGATA TCTTACTCAT TGAAACTATT 
TTTGATACCG CCAATGCCAA GGCAGCCTTG TTTGCAGTCC AAAAACTTTT TGAAGAGGAG 
TATGTTCCCC GGCCTGTCTT TATTTCAGGG ACAATTGTTG ATAAAAGTGG GCGGACTCTC 
TCTGGCCAGA CTGGGGAGGC ATTTGTCATC AGCGTGTCTC ATGCAGACCC ACTCTGCATT 
GGATTAAATT GTGCTCTGGG TGCAGCTGAA ATGAGACCTT TTATTGAAAC AATTGGAAAA 
TGTACAACAG CTTATGTTCT CTGTTATCCC AATGCAGGTC TTCCCAATAC CTTCGGTGAC 
TATGATGAAA CTCCCCACGT GATGGCCATG CATTTAAAGG ATTTTGCTGT TGATGGCTTG 
GTCAACATAG TTGGCGGATG CTGTGGTACG ACACCAGATC ATATCAGAGA AATTGCTGAA 
GCTGTGAAAA ACTGTAAGCC TAGAGTTCCA CCTGCCACTG TTTTCGAAGG GCATATGCTA 
CTGTCTGGTC TAGAGCCCTT CAGGATTGGA CCATACACCA ACTTTGTTAA CATTGGAGAG 
CGCTGTAACG TTGCAGGATC CAGGAGATTT GCTAAGCTCA TCATGGCAGG AAATTATGAA 
GAAGCACTGA GCGTTGCCAA AATGCAGGTG GAAATGGGAG CCCAGGTGTT GGATATTAAC 
ATGGATGATG GCATGCTGGA TGGTCCAAGT GCAATGACCA GATTCTGCAA CTTCATTGCT 
TCCGAGCCTG ACATTGCCAA GGTGCCCTTG TGCATTGACT CTTCCAATTT TGCTGTGATT 
GAAGCCGGCT TAAAGTGCTG CCAGGGGAAG TGCATTGTTA ATAGCATCAG CTTGAAGGAG 
GGAGAGGATG ACTTCTTGGA GAAGGCCAGA AAGATTAAGA AGTTTGGAGC TGCCGTGGTG 
GTTATGGCTT TTGATGAAGA AGGACAGGCA ACAGAAACAG ATACCAAAAT CAGAGTGTGC 
ACCCGGGCCT ACCATCTCCT TTTGAAAAAA CTGGGCTTCA ATCCAAATGA CATCATCTTT 
GACCCTAATA TCCTCACCAT TGGTACTGGG ATGGAAGAAC ATAACTTGTA TGCTGTTAAT 
TTTATCAATG CAACAAAAGT CATTAAAGAA ACGCTGCCTG GAGCCAAAGT AAGCGGTGGT 
CTTTCCAACT TGTCCTTCTC CTTCCGAGGA ATGGAAGCCA TTCGAGAGGC AATGCATGGG 
GTTTTCCTGT ACCATGCGAT CAAGTTTGGT ATGGACATGG GAATAGTGAA TGCTGGAAGC 
CTTCCTGTAT ATGATGACAT CCACAAGGAG CTTCTCCAGC TCTGTGAGGA CCTCATCTGG 
AACAGAGACC CTGAGGCCAC CGAGAAGCTC TTACACTACG CCCAGACTCA GGGCAAAGGA 
GGGAAGAAAG TCATCCAGAC TGATGAGTGG AGGAATGGCC CTCTCGAAGA ACGCCTTGAG 
TACGCTCTTG TGAAGGGCAT TGAAAAATAT ATTATTGAAG ATACTGAGGA AGCCAGGTTA 
AACCAGGAAA AATATCCCCG ACCTCTGAAT ATAATTGAAG GGCCGCTGAT GAATGGCATG 
AAAATTGTTG GTGATCTTTT TGGAGCTGGA AAAATGTTTC TACCTCAGGT TATAAAGTCA 
GCTCGGGTCA TGAAGAAGGC CGTGGGTCAC CTTATTCCCT TCATGGAAAA AGAGAGAGAA 
GAAACCAAAG TGCTTACTGG CAAAATAGAA GATGAGGACC CTTACCAAGG CACCATCGTG 
CTGGCTACTG TTAAAGGTGA CGTACACGAC ATAGGCAAGA ACATAGTTGG AGTGGTTCTT 
GGCTGCAATA ATTTCAGAGT TATTGATTTA GGAGTCATGA CTCCATGTGA CAAGATACTG 
AAGGCTGCTC TAGACCACAA AGCAGATATA ATTGGCCTGT CAGGACTCAT CACTCCTTCC 
CTGGATGAAA TGATTTTTGT CGCTAAGGAA ATGGAGAGAC TAGCTATAAA GATCCCATTG 
TTGATCGGAG GAGCTACCAC TTCCAGAACC CACACAGCAG TTAAAATAGC CCCAAGATAC 
AGTGCGCCCG TGATCCACGT CCTGGATGCA TCCAAGAGCG TGGTGGTGTG TTCTCAACTG 
TTAGATGAGA ATCTAAAAGA TGAGTACTTT GAGGAGATCC TGGAAGAATA TGAAGATATT 
AGACAGGACC ATTATGAATC TCTCAAGGAG AGAAGATACT TAACCTTAAG GCAAGCTAGA 
GAAAATGGTT TCCATATTGA CTGGCTGTCA GAGCCTCCTC CAGTGAAGCC CACGTTCCTC 
GGGACTCGGG TCTTTGAAGA CTACGACCTG CAGAAGCTGG TGGACTACAT TGATTGGAAG 
CCTTTCTTCG ACGTCTGGCA GCTCCGGGGC AAATACCCGA ACCGAGGCTT TCCCAAGATA 
TTTGACGACA AAACTGTAGG TGAAGAGGCC AAAAAGGTCT ATGATGATGC CCAAAATATG 
CTGCAGGCAC TGATCAGTCA GAAGAAGCTC CAGGCCAGGG GTGTGGTTGG GTTCTGGCCA 
GCACAGAGCA TTCAGGATGA TATCCACCTG TACGCGGAAG GCGCAGTGCC ACAGGCCTCG 
GAGCCCATAG CCACCTTCTA CGGGCTCAGG CAGCAGGCCG AGAAGGACTC TGCCAGCTCG 
GACCCCTACC TCTGCCTCTC GGACTTCATC GCCCCTCTGC ACTCAGGTAT CCCCGACTAC 
CTGGGCCTGT TCGCCGTTGC CTGCTTTGGG GTGGAGGAGC TGAGCAAGGC CTACGAGGAG 
GAGTGTGATG ACTACAGCAG CATCATGGTC AAGGCGCTGG GGGACCGGCT GGCCGAGGCC 
TTCGCTGAGG AGCTGCACGA GCGGGTCCGC AGGGAGCTGT GGGGTTACTG TAGTGGTGAG 
CAGCTGGCTG TGGCTGACCT GCGGAGACTG CGCTACGAGG GCATCCGGCC AGCGCCCGGC 
TATCCCAGCC AGCCTGACCA CACTGAGAAG CTGACCATGT GGAGGCTGGC GGACGTGGAG 
CAGCGCACAG GCATTAGGTT AACAGAATCG CTGGCCATGG CACCTGCTTC AGCAGTATCT 
GGCCTTTACT TCTCCAACTT GAAGTCCAAG TATTTTGCCG TGGGGAAAAT TTCCAAGGAT 
CAGATTGAGG ATTATGCTTC GAGGAAGAAC ATGTCTGTGG CCGAGGTTGA GAAATGGCTT 
GGACCCATTT TGGGATATGA TACAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR ( NM_001030298.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001025469.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), mRNA.

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CloneID	OBa42210
Clone ID Related Accession (Same CDS sequence)	NM_001030298.1
Accession Version	NM_001030298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	methionine synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ084519.1. On or before Aug 7, 2005 this sequence version replaced XM_598676.1, XM_615180.1. ##Evidence-Data-START## Transcript exon combination :: DQ084519.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCCA CGCTCCAGGA CCTGACGCCA TCTGCAGGGA TGAAGAAGAC CCTGCAAGAT 
GAGATTGAAG CTATCCTGCA GGAGAGGATT ATGGTGCTTG ATGGAGGGAT GGGGACCATG 
ATCCAGCGGC ACAAGCTGAG TGAAGAAGAC TTCCGAGGAC AAGAATTTAA AGATCATGCC 
AGGCCCCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCCAATGT CATTTACCAA 
ATCCATAAGG AGTACCTCCT AGCTGGGGCA GATATCATTG AAACAAATAC TTTCAGCAGC 
ACTTCTATTG CCCAAGCTGA CTATGGCCTG GAACACTTGG CCTACCGGAT GAACATGTGC 
TCTGCGGGAG TGGCCAGAAA AGCAGCCGAG GACATCTCTC TCCAGACAGG AATTAAGAGG 
TATGTGGCAG GAGCTCTGGG TCCAACTAAC AAGACACTCT CTGTGTCGCC ATCTGTGGAA 
AGACCAGATT ACAGGAACAT CACATTTGAT GAACTTGTTG AAGCATACAA AGAGCAGGCC 
AAAGGGCTTC TGGATGGTGG AGTGGATATC TTACTCATTG AAACTATTTT TGATACCGCC 
AATGCCAAGG CAGCCTTGTT TGCAGTCCAA AAACTTTTTG AAGAGGAGTA TGTTCCCCGG 
CCTGTCTTTA TTTCAGGGAC AATTGTTGAT AAAAGTGGGC GGACTCTCTC TGGCCAGACT 
GGGGAGGCAT TTGTCATCAG CGTGTCTCAT GCAGACCCAC TCTGCATTGG ATTAAATTGT 
GCTCTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAACAA TTGGAAAATG TACAACAGCT 
TATGTTCTCT GTTATCCCAA TGCAGGTCTT CCCAATACCT TCGGTGACTA TGATGAAACT 
CCCCACGTGA TGGCCATGCA TTTAAAGGAT TTTGCTGTTG ATGGCTTGGT CAACATAGTT 
GGCGGATGCT GTGGTACGAC ACCAGATCAT ATCAGAGAAA TTGCTGAAGC TGTGAAAAAC 
TGTAAGCCTA GAGTTCCACC TGCCACTGTT TTCGAAGGGC ATATGCTACT GTCTGGTCTA 
GAGCCCTTCA GGATTGGACC ATACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAACGTT 
GCAGGATCCA GGAGATTTGC TAAGCTCATC ATGGCAGGAA ATTATGAAGA AGCACTGAGC 
GTTGCCAAAA TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATATTAACAT GGATGATGGC 
ATGCTGGATG GTCCAAGTGC AATGACCAGA TTCTGCAACT TCATTGCTTC CGAGCCTGAC 
ATTGCCAAGG TGCCCTTGTG CATTGACTCT TCCAATTTTG CTGTGATTGA AGCCGGCTTA 
AAGTGCTGCC AGGGGAAGTG CATTGTTAAT AGCATCAGCT TGAAGGAGGG AGAGGATGAC 
TTCTTGGAGA AGGCCAGAAA GATTAAGAAG TTTGGAGCTG CCGTGGTGGT TATGGCTTTT 
GATGAAGAAG GACAGGCAAC AGAAACAGAT CCCAAAATCA GAGTGTGCAC CCGGGCCTAC 
CATCTCCTTT TGAAAAAACT GGGCTTCAAT CCAAATGACA TCATCTTTGA CCCTAATATC 
CTCACCATTG GTACTGGGAT GGAAGAACAT AACTTGTATG CTGTTAATTT TATCAATGCA 
ACAAAAGTCA TTAAAGAAAC GCTGCCTGGA GCCAAAGTAA GCGGTGGTCT TTCCAACTTG 
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAGGCAA TGCATGGGGT TTTCCTGTAC 
CATGCGATCA AGTTTGGTAT GGACATGGGA ATAGTGAATG CTGGAAGCCT TCCTGTATAT 
GATGACATCC ACAAGGAGCT TCTCCAGCTC TGTGAGGACC TCATCTGGAA CAGAGACCCT 
GAGGCCACCG AGAAGCTCTT ACACTACGCC CAGACTCAGG GCAAAGGAGG GAAGAAAGTC 
ATCCAGACTG ATGAGTGGAG GAATGGCCCT CTCGAAGAAC GCCTTGAGTA CGCTCTTGTG 
AAGGGCATTG AAAAATATAT TATTGAAGAT ACTGAGGAAG CCAGGTTAAA CCAGGAAAAA 
TATCCCCGAC CTCTGAATAT AATTGAAGGG CCGCTGATGA ATGGCATGAA AATTGTTGGT 
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC TCGGGTCATG 
AAGAAGGCCG TGGGTCACCT TATTCCCTTC ATGGAAAAAG AGAGAGAAGA AACCAAAGTG 
CTTACTGGCA AAATAGAAGA TGAGGACCCT TACCAAGGCA CCATCGTGCT GGCTACTGTT 
AAAGGTGACG TACACGACAT AGGCAAGAAC ATAGTTGGAG TGGTTCTTGG CTGCAATAAT 
TTCAGAGTTA TTGATTTAGG AGTCATGACT CCATGTGACA AGATACTGAA GGCTGCTCTA 
GACCACAAAG CAGATATAAT TGGCCTGTCA GGACTCATCA CTCCTTCCCT GGATGAAATG 
ATTTTTGTCG CTAAGGAAAT GGAGAGACTA GCTATAAAGA TCCCATTGTT GATCGGAGGA 
GCTACCACTT CCAGAACCCA CACAGCAGTT AAAATAGCCC CAAGATACAG TGCGCCCGTG 
ATCCACGTCC TGGATGCATC CAAGAGCGTG GTGGTGTGTT CTCAACTGTT AGATGAGAAT 
CTAAAAGATG AGTACTTTGA GGAGATCCTG GAAGAATATG AAGATATTAG ACAGGACCAT 
TATGAATCTC TCAAGGAGAG AAGATACTTA ACCTTAAGGC AAGCTAGAGA AAATGGTTTC 
CATATTGACT GGCTGTCAGA GCCTCCTCCA GTGAAGCCCA CGTTCCTCGG GACTCGGGTC 
TTTGAAGACT ACGACCTGCA GAAGCTGGTG GACTACATTG ATTGGAAGCC TTTCTTCGAC 
GTCTGGCAGC TCCGGGGCAA ATACCCGAAC CGAGGCTTTC CCAAGATATT TGACGACAAA 
ACTGTAGGTG AAGAGGCCAA AAAGGTCTAT GATGATGCCC AAAATATGCT GCAGGCACTG 
ATCAGTCAGA AGAAGCTCCA GGCCAGGGGT GTGGTTGGGT TCTGGCCAGC ACAGAGCATT 
CAGGATGATA TCCACCTGTA CGCGGAAGGC GCAGTGCCAC AGGCCTCGGA GCCCATAGCC 
ACCTTCTACG GGCTCAGGCA GCAGGCCGAG AAGGACTCTG CCAGCTCGGA CCCCTACCTC 
TGCCTCTCGG ACTTCATCGC CCCTCTGCAC TCAGGTATCC CCGACTACCT GGGCCTGTTC 
GCCGTTGCCT GCTTTGGGGT GGAGGAGCTG AGCAAGGCCT ACGAGGAGGA GTGTGATGAC 
TACAGCAGCA TCATGGTCAA GGCGCTGGGG GACCGGCTGG CCGAGGCCTT CGCTGAGGAG 
CTGCACGAGC GGGCCCGCAG GGAGCTGTGG GGTTACTGTA GTGGTGAGCA GCTGGCTGTG 
GCTGACCTGC GGAGACTGCG CTACGAGGGC ATCCGGCCGG CGCCCGGCTA TCCCAGCCAG 
CCTGACCACA CTGAGAAGCT GACCGTGTGG AGGCTGGCGG ACGTGGAGCA GCGCACAGGC 
ATTAGGTTAA CAGAATCGCT GGCCATGGCA CCTGCTTCAG CAGTATCTGG CCTTTACTTC 
TCCAACTTGA AGTCCAAGTA TTTTGCCGTG GGGAAAATTT CCAAGGATCA GATTGAGGAT 
TATGCTTCGA GGAAGAACAT GTCTGTGGCC GAGGTTGAGA AATGGCTTGG ACCCATTTTG 
GGATATGATA CAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025469.1
CDS	22..3819
Translation	MAPTLQDLTPSAGMKKTLQDEIEAILQERIMVLDGGMGTMIQRHKLSEEDFRGQEFKDHARPLKGNNDILSITQPNVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEDISLQTGIKRYVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYKEQAKGLLDGGVDILLIETIFDTANAKAALFAVQKLFEEEYVPRPVFISGTIVDKSGRTLSGQTGEAFVISVSHADPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPHVMAMHLKDFAVDGLVNIVGGCCGTTPDHIREIAEAVKNCKPRVPPATVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRRFAKLIMAGNYEEALSVAKMQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDPKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERARRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTVWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	NM_001030298.1
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030298.1

ORF Insert Sequence:

ATGGCCCCCA CGCTCCAGGA CCTGACGCCA TCTGCAGGGA TGAAGAAGAC CCTGCAAGAT 
GAGATTGAAG CTATCCTGCA GGAGAGGATT ATGGTGCTTG ATGGAGGGAT GGGGACCATG 
ATCCAGCGGC ACAAGCTGAG TGAAGAAGAC TTCCGAGGAC AAGAATTTAA AGATCATGCC 
AGGCCCCTGA AAGGCAACAA TGACATTTTA AGTATAACTC AGCCCAATGT CATTTACCAA 
ATCCATAAGG AGTACCTCCT AGCTGGGGCA GATATCATTG AAACAAATAC TTTCAGCAGC 
ACTTCTATTG CCCAAGCTGA CTATGGCCTG GAACACTTGG CCTACCGGAT GAACATGTGC 
TCTGCGGGAG TGGCCAGAAA AGCAGCCGAG GACATCTCTC TCCAGACAGG AATTAAGAGG 
TATGTGGCAG GAGCTCTGGG TCCAACTAAC AAGACACTCT CTGTGTCGCC ATCTGTGGAA 
AGACCAGATT ACAGGAACAT CACATTTGAT GAACTTGTTG AAGCATACAA AGAGCAGGCC 
AAAGGGCTTC TGGATGGTGG AGTGGATATC TTACTCATTG AAACTATTTT TGATACCGCC 
AATGCCAAGG CAGCCTTGTT TGCAGTCCAA AAACTTTTTG AAGAGGAGTA TGTTCCCCGG 
CCTGTCTTTA TTTCAGGGAC AATTGTTGAT AAAAGTGGGC GGACTCTCTC TGGCCAGACT 
GGGGAGGCAT TTGTCATCAG CGTGTCTCAT GCAGACCCAC TCTGCATTGG ATTAAATTGT 
GCTCTGGGTG CAGCTGAAAT GAGACCTTTT ATTGAAACAA TTGGAAAATG TACAACAGCT 
TATGTTCTCT GTTATCCCAA TGCAGGTCTT CCCAATACCT TCGGTGACTA TGATGAAACT 
CCCCACGTGA TGGCCATGCA TTTAAAGGAT TTTGCTGTTG ATGGCTTGGT CAACATAGTT 
GGCGGATGCT GTGGTACGAC ACCAGATCAT ATCAGAGAAA TTGCTGAAGC TGTGAAAAAC 
TGTAAGCCTA GAGTTCCACC TGCCACTGTT TTCGAAGGGC ATATGCTACT GTCTGGTCTA 
GAGCCCTTCA GGATTGGACC ATACACCAAC TTTGTTAACA TTGGAGAGCG CTGTAACGTT 
GCAGGATCCA GGAGATTTGC TAAGCTCATC ATGGCAGGAA ATTATGAAGA AGCACTGAGC 
GTTGCCAAAA TGCAGGTGGA AATGGGAGCC CAGGTGTTGG ATATTAACAT GGATGATGGC 
ATGCTGGATG GTCCAAGTGC AATGACCAGA TTCTGCAACT TCATTGCTTC CGAGCCTGAC 
ATTGCCAAGG TGCCCTTGTG CATTGACTCT TCCAATTTTG CTGTGATTGA AGCCGGCTTA 
AAGTGCTGCC AGGGGAAGTG CATTGTTAAT AGCATCAGCT TGAAGGAGGG AGAGGATGAC 
TTCTTGGAGA AGGCCAGAAA GATTAAGAAG TTTGGAGCTG CCGTGGTGGT TATGGCTTTT 
GATGAAGAAG GACAGGCAAC AGAAACAGAT CCCAAAATCA GAGTGTGCAC CCGGGCCTAC 
CATCTCCTTT TGAAAAAACT GGGCTTCAAT CCAAATGACA TCATCTTTGA CCCTAATATC 
CTCACCATTG GTACTGGGAT GGAAGAACAT AACTTGTATG CTGTTAATTT TATCAATGCA 
ACAAAAGTCA TTAAAGAAAC GCTGCCTGGA GCCAAAGTAA GCGGTGGTCT TTCCAACTTG 
TCCTTCTCCT TCCGAGGAAT GGAAGCCATT CGAGAGGCAA TGCATGGGGT TTTCCTGTAC 
CATGCGATCA AGTTTGGTAT GGACATGGGA ATAGTGAATG CTGGAAGCCT TCCTGTATAT 
GATGACATCC ACAAGGAGCT TCTCCAGCTC TGTGAGGACC TCATCTGGAA CAGAGACCCT 
GAGGCCACCG AGAAGCTCTT ACACTACGCC CAGACTCAGG GCAAAGGAGG GAAGAAAGTC 
ATCCAGACTG ATGAGTGGAG GAATGGCCCT CTCGAAGAAC GCCTTGAGTA CGCTCTTGTG 
AAGGGCATTG AAAAATATAT TATTGAAGAT ACTGAGGAAG CCAGGTTAAA CCAGGAAAAA 
TATCCCCGAC CTCTGAATAT AATTGAAGGG CCGCTGATGA ATGGCATGAA AATTGTTGGT 
GATCTTTTTG GAGCTGGAAA AATGTTTCTA CCTCAGGTTA TAAAGTCAGC TCGGGTCATG 
AAGAAGGCCG TGGGTCACCT TATTCCCTTC ATGGAAAAAG AGAGAGAAGA AACCAAAGTG 
CTTACTGGCA AAATAGAAGA TGAGGACCCT TACCAAGGCA CCATCGTGCT GGCTACTGTT 
AAAGGTGACG TACACGACAT AGGCAAGAAC ATAGTTGGAG TGGTTCTTGG CTGCAATAAT 
TTCAGAGTTA TTGATTTAGG AGTCATGACT CCATGTGACA AGATACTGAA GGCTGCTCTA 
GACCACAAAG CAGATATAAT TGGCCTGTCA GGACTCATCA CTCCTTCCCT GGATGAAATG 
ATTTTTGTCG CTAAGGAAAT GGAGAGACTA GCTATAAAGA TCCCATTGTT GATCGGAGGA 
GCTACCACTT CCAGAACCCA CACAGCAGTT AAAATAGCCC CAAGATACAG TGCGCCCGTG 
ATCCACGTCC TGGATGCATC CAAGAGCGTG GTGGTGTGTT CTCAACTGTT AGATGAGAAT 
CTAAAAGATG AGTACTTTGA GGAGATCCTG GAAGAATATG AAGATATTAG ACAGGACCAT 
TATGAATCTC TCAAGGAGAG AAGATACTTA ACCTTAAGGC AAGCTAGAGA AAATGGTTTC 
CATATTGACT GGCTGTCAGA GCCTCCTCCA GTGAAGCCCA CGTTCCTCGG GACTCGGGTC 
TTTGAAGACT ACGACCTGCA GAAGCTGGTG GACTACATTG ATTGGAAGCC TTTCTTCGAC 
GTCTGGCAGC TCCGGGGCAA ATACCCGAAC CGAGGCTTTC CCAAGATATT TGACGACAAA 
ACTGTAGGTG AAGAGGCCAA AAAGGTCTAT GATGATGCCC AAAATATGCT GCAGGCACTG 
ATCAGTCAGA AGAAGCTCCA GGCCAGGGGT GTGGTTGGGT TCTGGCCAGC ACAGAGCATT 
CAGGATGATA TCCACCTGTA CGCGGAAGGC GCAGTGCCAC AGGCCTCGGA GCCCATAGCC 
ACCTTCTACG GGCTCAGGCA GCAGGCCGAG AAGGACTCTG CCAGCTCGGA CCCCTACCTC 
TGCCTCTCGG ACTTCATCGC CCCTCTGCAC TCAGGTATCC CCGACTACCT GGGCCTGTTC 
GCCGTTGCCT GCTTTGGGGT GGAGGAGCTG AGCAAGGCCT ACGAGGAGGA GTGTGATGAC 
TACAGCAGCA TCATGGTCAA GGCGCTGGGG GACCGGCTGG CCGAGGCCTT CGCTGAGGAG 
CTGCACGAGC GGGCCCGCAG GGAGCTGTGG GGTTACTGTA GTGGTGAGCA GCTGGCTGTG 
GCTGACCTGC GGAGACTGCG CTACGAGGGC ATCCGGCCGG CGCCCGGCTA TCCCAGCCAG 
CCTGACCACA CTGAGAAGCT GACCGTGTGG AGGCTGGCGG ACGTGGAGCA GCGCACAGGC 
ATTAGGTTAA CAGAATCGCT GGCCATGGCA CCTGCTTCAG CAGTATCTGG CCTTTACTTC 
TCCAACTTGA AGTCCAAGTA TTTTGCCGTG GGGAAAATTT CCAAGGATCA GATTGAGGAT 
TATGCTTCGA GGAAGAACAT GTCTGTGGCC GAGGTTGAGA AATGGCTTGG ACCCATTTTG 
GGATATGATA CAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR ( XM_010820411.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818713.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.

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CloneID	OBa45029
Clone ID Related Accession (Same CDS sequence)	XM_010820411.2 , XM_010820411.3
Accession Version	XM_010820411.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	methionine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104619.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCA CGCTCCAGGA CCTGACGCCA TCTGGGATGA AGAAGACCCT GCAAGATGAG 
ATTGAAGCTA TCCTGCAGGA GAGGATTATG GTGCTTGATG GAGGGATGGG GACCATGATC 
CAGCAGCACA AGCTGAGTGA AGAAGACTTC CGAGGACAAG AATTTAAAGA TCATGCCAGG 
CCCCTGAAAG GCAACAATGA CATTTTAAGT ATAACTCAGC CCAGTGTCAT TTACCAAATC 
CATAAGGAGT ACCTCCTAGC TGGGGCAGAT ATCATTGAAA CAAATACTTT CAGCAGCACT 
TCTATTGCCC AAGCTGACTA TGGCCTGGAA CACTTGGCCT ACCGGATGAA CATGTGCTCT 
GCGGGAGTGG CCAGAAAAGC AGCCGAGGAC ATCTCTCTCC AGACAGGAAT TAAGAGGTAT 
GTGGCAGGAG CTCTGGGTCC AACTAACAAG ACACTCTCTG TGTCGCCATC TGTGGAAAGA 
CCAGATTACA GGAACATCAC ATTTGATGAA CTTGTTGAAG CATACAAAGA GCAGGCCAAA 
GGGCTTCTGG ATGGTGGAGT GGATATCTTA CTCATTGAAA CTATTTTTGA TACCGCCAAT 
GCCAAGGCAG CCTTGTTTGC AGTCCAAAAA CTTTTTGAAG AGGAGTATGT TCCCCGGCCT 
GTCTTTATTT CAGGGACAAT TGTTGATAAA AGTGGGCGGA CTCTCTCTGG CCAGACTGGG 
GAGGCATTTG TCATCAGCGT GTCTCATGCA GACCCACTCT GCATTGGATT AAATTGTGCT 
CTGGGTGCAG CTGAAATGAG ACCTTTTATT GAAACAATTG GAAAATGTAC AACAGCTTAT 
GTTCTCTGTT ATCCCAATGC AGGTCTTCCC AATACCTTCG GTGACTATGA TGAAACTCCC 
CACGTGATGG CCATGCATTT AAAGGATTTT GCTGTTGATG GCTTGGTCAA CATAGTTGGC 
GGATGCTGTG GTACGACACC AGATCATATC AGAGAAATTG CTGAAGCTGT GAAAAACTGT 
AAGCCTAGAG TTCCACCTGC CACTGTTTTC GAAGGGCATA TGCTACTGTC TGGTCTAGAG 
CCCTTCAGGA TTGGACCATA CACCAACTTT GTTAACATTG GAGAGCGCTG TAACGTTGCA 
GGATCCAGGA GATTTGCTAA GCTCATCATG GCAGGAAATT ATGAAGAAGC ACTGAGCGTT 
GCCAAAATGC AGGTGGAAAT GGGAGCCCAG GTGTTGGATA TTAACATGGA TGATGGCATG 
CTGGATGGTC CAAGTGCAAT GACCAGATTC TGCAACTTCA TTGCTTCCGA GCCTGACATT 
GCCAAGGTGC CCTTGTGCAT TGACTCTTCC AATTTTGCTG TGATTGAAGC CGGCTTAAAG 
TGCTGCCAGG GGAAGTGCAT TGTTAATAGC ATCAGCTTGA AGGAGGGAGA GGATGACTTC 
TTGGAGAAGG CCAGAAAGAT TAAGAAGTTT GGAGCTGCCG TGGTGGTTAT GGCTTTTGAT 
GAAGAAGGAC AGGCAACAGA AACAGATACC AAAATCAGAG TGTGCACCCG GGCCTACCAT 
CTCCTTTTGA AAAAACTGGG CTTCAATCCA AATGACATCA TCTTTGACCC TAATATCCTC 
ACCATTGGTA CTGGGATGGA AGAACATAAC TTGTATGCTG TTAATTTTAT CAATGCAACA 
AAAGTCATTA AAGAAACGCT GCCTGGAGCC AAAGTAAGCG GTGGTCTTTC CAACTTGTCC 
TTCTCCTTCC GAGGAATGGA AGCCATTCGA GAGGCAATGC ATGGGGTTTT CCTGTACCAT 
GCGATCAAGT TTGGTATGGA CATGGGAATA GTGAATGCTG GAAGCCTTCC TGTATATGAT 
GACATCCACA AGGAGCTTCT CCAGCTCTGT GAGGACCTCA TCTGGAACAG AGACCCTGAG 
GCCACCGAGA AGCTCTTACA CTACGCCCAG ACTCAGGGCA AAGGAGGGAA GAAAGTCATC 
CAGACTGATG AGTGGAGGAA TGGCCCTCTC GAAGAACGCC TTGAGTACGC TCTTGTGAAG 
GGCATTGAAA AATATATTAT TGAAGATACT GAGGAAGCCA GGTTAAACCA GGAAAAATAT 
CCCCGACCTC TGAATATAAT TGAAGGGCCG CTGATGAATG GCATGAAAAT TGTTGGTGAT 
CTTTTTGGAG CTGGAAAAAT GTTTCTACCT CAGGTTATAA AGTCAGCTCG GGTCATGAAG 
AAGGCCGTGG GTCACCTTAT TCCCTTCATG GAAAAAGAGA GAGAAGAAAC CAAAGTGCTT 
ACTGGCAAAA TAGAAGATGA GGACCCTTAC CAAGGCACCA TCGTGCTGGC TACTGTTAAA 
GGTGACGTAC ACGACATAGG CAAGAACATA GTTGGAGTGG TTCTTGGCTG CAATAATTTC 
AGAGTTATTG ATTTAGGAGT CATGACTCCA TGTGACAAGA TACTGAAGGC TGCTCTAGAC 
CACAAAGCAG ATATAATTGG CCTGTCAGGA CTCATCACTC CTTCCCTGGA TGAAATGATT 
TTTGTCGCTA AGGAAATGGA GAGACTAGCT ATAAAGATCC CATTGTTGAT CGGAGGAGCT 
ACCACTTCCA GAACCCACAC AGCAGTTAAA ATAGCCCCAA GATACAGTGC GCCCGTGATC 
CACGTCCTGG ATGCATCCAA GAGCGTGGTG GTGTGTTCTC AACTGTTAGA TGAGAATCTA 
AAAGATGAGT ACTTTGAGGA GATCCTGGAA GAATATGAAG ATATTAGACA GGACCATTAT 
GAATCTCTCA AGGAGAGAAG ATACTTAACC TTAAGGCAAG CTAGAGAAAA TGGTTTCCAT 
ATTGACTGGC TGTCAGAGCC TCCTCCAGTG AAGCCCACGT TCCTCGGGAC TCGGGTCTTT 
GAAGACTACG ACCTGCAGAA GCTGGTGGAC TACATTGATT GGAAGCCTTT CTTCGACGTC 
TGGCAGCTCC GGGGCAAATA CCCGAACCGA GGCTTTCCCA AGATATTTGA CGACAAAACT 
GTAGGTGAAG AGGCCAAAAA GGTCTATGAT GATGCCCAAA ATATGCTGCA GGCACTGATC 
AGTCAGAAGA AGCTCCAGGC CAGGGGTGTG GTTGGGTTCT GGCCAGCACA GAGCATTCAG 
GATGATATCC ACCTGTACGC GGAAGGCGCA GTGCCACAGG CCTCGGAGCC CATAGCCACC 
TTCTACGGGC TCAGGCAGCA GGCCGAGAAG GACTCTGCCA GCTCGGACCC CTACCTCTGC 
CTCTCGGACT TCATCGCCCC TCTGCACTCA GGTATCCCCG ACTACCTGGG CCTGTTCGCC 
GTTGCCTGCT TTGGGGTGGA GGAGCTGAGC AAGGCCTACG AGGAGGAGTG TGATGACTAC 
AGCAGCATCA TGGTCAAGGC GCTGGGGGAC CGGCTGGCCG AGGCCTTCGC TGAGGAGCTG 
CACGAGCGGG TCCGCAGGGA GCTGTGGGGT TACTGTAGTG GTGAGCAGCT GGCTGTGGCT 
GACCTGCGGA GACTGCGCTA CGAGGGCATC CGGCCAGCGC CCGGCTATCC CAGCCAGCCT 
GACCACACTG AGAAGCTGAC CATGTGGAGG CTGGCGGACG TGGAGCAGCG CACAGGCATT 
AGGTTAACAG AATCGCTGGC CATGGCACCT GCTTCAGCAG TATCTGGCCT TTACTTCTCC 
AACTTGAAGT CCAAGTATTT TGCCGTGGGG AAAATTTCCA AGGATCAGAT TGAGGATTAT 
GCTTCGAGGA AGAACATGTC TGTGGCCGAG GTTGAGAAAT GGCTTGGACC CATTTTGGGA 
TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818713.1
CDS	451..4245
Translation	MAPTLQDLTPSGMKKTLQDEIEAILQERIMVLDGGMGTMIQQHKLSEEDFRGQEFKDHARPLKGNNDILSITQPSVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEDISLQTGIKRYVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYKEQAKGLLDGGVDILLIETIFDTANAKAALFAVQKLFEEEYVPRPVFISGTIVDKSGRTLSGQTGEAFVISVSHADPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPHVMAMHLKDFAVDGLVNIVGGCCGTTPDHIREIAEAVKNCKPRVPPATVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRRFAKLIMAGNYEEALSVAKMQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDTKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_010820411.2
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820411.2

ORF Insert Sequence:

ATGGCCCCCA CGCTCCAGGA CCTGACGCCA TCTGGGATGA AGAAGACCCT GCAAGATGAG 
ATTGAAGCTA TCCTGCAGGA GAGGATTATG GTGCTTGATG GAGGGATGGG GACCATGATC 
CAGCAGCACA AGCTGAGTGA AGAAGACTTC CGAGGACAAG AATTTAAAGA TCATGCCAGG 
CCCCTGAAAG GCAACAATGA CATTTTAAGT ATAACTCAGC CCAGTGTCAT TTACCAAATC 
CATAAGGAGT ACCTCCTAGC TGGGGCAGAT ATCATTGAAA CAAATACTTT CAGCAGCACT 
TCTATTGCCC AAGCTGACTA TGGCCTGGAA CACTTGGCCT ACCGGATGAA CATGTGCTCT 
GCGGGAGTGG CCAGAAAAGC AGCCGAGGAC ATCTCTCTCC AGACAGGAAT TAAGAGGTAT 
GTGGCAGGAG CTCTGGGTCC AACTAACAAG ACACTCTCTG TGTCGCCATC TGTGGAAAGA 
CCAGATTACA GGAACATCAC ATTTGATGAA CTTGTTGAAG CATACAAAGA GCAGGCCAAA 
GGGCTTCTGG ATGGTGGAGT GGATATCTTA CTCATTGAAA CTATTTTTGA TACCGCCAAT 
GCCAAGGCAG CCTTGTTTGC AGTCCAAAAA CTTTTTGAAG AGGAGTATGT TCCCCGGCCT 
GTCTTTATTT CAGGGACAAT TGTTGATAAA AGTGGGCGGA CTCTCTCTGG CCAGACTGGG 
GAGGCATTTG TCATCAGCGT GTCTCATGCA GACCCACTCT GCATTGGATT AAATTGTGCT 
CTGGGTGCAG CTGAAATGAG ACCTTTTATT GAAACAATTG GAAAATGTAC AACAGCTTAT 
GTTCTCTGTT ATCCCAATGC AGGTCTTCCC AATACCTTCG GTGACTATGA TGAAACTCCC 
CACGTGATGG CCATGCATTT AAAGGATTTT GCTGTTGATG GCTTGGTCAA CATAGTTGGC 
GGATGCTGTG GTACGACACC AGATCATATC AGAGAAATTG CTGAAGCTGT GAAAAACTGT 
AAGCCTAGAG TTCCACCTGC CACTGTTTTC GAAGGGCATA TGCTACTGTC TGGTCTAGAG 
CCCTTCAGGA TTGGACCATA CACCAACTTT GTTAACATTG GAGAGCGCTG TAACGTTGCA 
GGATCCAGGA GATTTGCTAA GCTCATCATG GCAGGAAATT ATGAAGAAGC ACTGAGCGTT 
GCCAAAATGC AGGTGGAAAT GGGAGCCCAG GTGTTGGATA TTAACATGGA TGATGGCATG 
CTGGATGGTC CAAGTGCAAT GACCAGATTC TGCAACTTCA TTGCTTCCGA GCCTGACATT 
GCCAAGGTGC CCTTGTGCAT TGACTCTTCC AATTTTGCTG TGATTGAAGC CGGCTTAAAG 
TGCTGCCAGG GGAAGTGCAT TGTTAATAGC ATCAGCTTGA AGGAGGGAGA GGATGACTTC 
TTGGAGAAGG CCAGAAAGAT TAAGAAGTTT GGAGCTGCCG TGGTGGTTAT GGCTTTTGAT 
GAAGAAGGAC AGGCAACAGA AACAGATACC AAAATCAGAG TGTGCACCCG GGCCTACCAT 
CTCCTTTTGA AAAAACTGGG CTTCAATCCA AATGACATCA TCTTTGACCC TAATATCCTC 
ACCATTGGTA CTGGGATGGA AGAACATAAC TTGTATGCTG TTAATTTTAT CAATGCAACA 
AAAGTCATTA AAGAAACGCT GCCTGGAGCC AAAGTAAGCG GTGGTCTTTC CAACTTGTCC 
TTCTCCTTCC GAGGAATGGA AGCCATTCGA GAGGCAATGC ATGGGGTTTT CCTGTACCAT 
GCGATCAAGT TTGGTATGGA CATGGGAATA GTGAATGCTG GAAGCCTTCC TGTATATGAT 
GACATCCACA AGGAGCTTCT CCAGCTCTGT GAGGACCTCA TCTGGAACAG AGACCCTGAG 
GCCACCGAGA AGCTCTTACA CTACGCCCAG ACTCAGGGCA AAGGAGGGAA GAAAGTCATC 
CAGACTGATG AGTGGAGGAA TGGCCCTCTC GAAGAACGCC TTGAGTACGC TCTTGTGAAG 
GGCATTGAAA AATATATTAT TGAAGATACT GAGGAAGCCA GGTTAAACCA GGAAAAATAT 
CCCCGACCTC TGAATATAAT TGAAGGGCCG CTGATGAATG GCATGAAAAT TGTTGGTGAT 
CTTTTTGGAG CTGGAAAAAT GTTTCTACCT CAGGTTATAA AGTCAGCTCG GGTCATGAAG 
AAGGCCGTGG GTCACCTTAT TCCCTTCATG GAAAAAGAGA GAGAAGAAAC CAAAGTGCTT 
ACTGGCAAAA TAGAAGATGA GGACCCTTAC CAAGGCACCA TCGTGCTGGC TACTGTTAAA 
GGTGACGTAC ACGACATAGG CAAGAACATA GTTGGAGTGG TTCTTGGCTG CAATAATTTC 
AGAGTTATTG ATTTAGGAGT CATGACTCCA TGTGACAAGA TACTGAAGGC TGCTCTAGAC 
CACAAAGCAG ATATAATTGG CCTGTCAGGA CTCATCACTC CTTCCCTGGA TGAAATGATT 
TTTGTCGCTA AGGAAATGGA GAGACTAGCT ATAAAGATCC CATTGTTGAT CGGAGGAGCT 
ACCACTTCCA GAACCCACAC AGCAGTTAAA ATAGCCCCAA GATACAGTGC GCCCGTGATC 
CACGTCCTGG ATGCATCCAA GAGCGTGGTG GTGTGTTCTC AACTGTTAGA TGAGAATCTA 
AAAGATGAGT ACTTTGAGGA GATCCTGGAA GAATATGAAG ATATTAGACA GGACCATTAT 
GAATCTCTCA AGGAGAGAAG ATACTTAACC TTAAGGCAAG CTAGAGAAAA TGGTTTCCAT 
ATTGACTGGC TGTCAGAGCC TCCTCCAGTG AAGCCCACGT TCCTCGGGAC TCGGGTCTTT 
GAAGACTACG ACCTGCAGAA GCTGGTGGAC TACATTGATT GGAAGCCTTT CTTCGACGTC 
TGGCAGCTCC GGGGCAAATA CCCGAACCGA GGCTTTCCCA AGATATTTGA CGACAAAACT 
GTAGGTGAAG AGGCCAAAAA GGTCTATGAT GATGCCCAAA ATATGCTGCA GGCACTGATC 
AGTCAGAAGA AGCTCCAGGC CAGGGGTGTG GTTGGGTTCT GGCCAGCACA GAGCATTCAG 
GATGATATCC ACCTGTACGC GGAAGGCGCA GTGCCACAGG CCTCGGAGCC CATAGCCACC 
TTCTACGGGC TCAGGCAGCA GGCCGAGAAG GACTCTGCCA GCTCGGACCC CTACCTCTGC 
CTCTCGGACT TCATCGCCCC TCTGCACTCA GGTATCCCCG ACTACCTGGG CCTGTTCGCC 
GTTGCCTGCT TTGGGGTGGA GGAGCTGAGC AAGGCCTACG AGGAGGAGTG TGATGACTAC 
AGCAGCATCA TGGTCAAGGC GCTGGGGGAC CGGCTGGCCG AGGCCTTCGC TGAGGAGCTG 
CACGAGCGGG TCCGCAGGGA GCTGTGGGGT TACTGTAGTG GTGAGCAGCT GGCTGTGGCT 
GACCTGCGGA GACTGCGCTA CGAGGGCATC CGGCCAGCGC CCGGCTATCC CAGCCAGCCT 
GACCACACTG AGAAGCTGAC CATGTGGAGG CTGGCGGACG TGGAGCAGCG CACAGGCATT 
AGGTTAACAG AATCGCTGGC CATGGCACCT GCTTCAGCAG TATCTGGCCT TTACTTCTCC 
AACTTGAAGT CCAAGTATTT TGCCGTGGGG AAAATTTCCA AGGATCAGAT TGAGGATTAT 
GCTTCGAGGA AGAACATGTC TGTGGCCGAG GTTGAGAAAT GGCTTGGACC CATTTTGGGA 
TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR ( XM_010820411.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818713.1 Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.

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CloneID	OBa45029
Clone ID Related Accession (Same CDS sequence)	XM_010820411.2 , XM_010820411.3
Accession Version	XM_010820411.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	methionine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCA CGCTCCAGGA CCTGACGCCA TCTGGGATGA AGAAGACCCT GCAAGATGAG 
ATTGAAGCTA TCCTGCAGGA GAGGATTATG GTGCTTGATG GAGGGATGGG GACCATGATC 
CAGCAGCACA AGCTGAGTGA AGAAGACTTC CGAGGACAAG AATTTAAAGA TCATGCCAGG 
CCCCTGAAAG GCAACAATGA CATTTTAAGT ATAACTCAGC CCAGTGTCAT TTACCAAATC 
CATAAGGAGT ACCTCCTAGC TGGGGCAGAT ATCATTGAAA CAAATACTTT CAGCAGCACT 
TCTATTGCCC AAGCTGACTA TGGCCTGGAA CACTTGGCCT ACCGGATGAA CATGTGCTCT 
GCGGGAGTGG CCAGAAAAGC AGCCGAGGAC ATCTCTCTCC AGACAGGAAT TAAGAGGTAT 
GTGGCAGGAG CTCTGGGTCC AACTAACAAG ACACTCTCTG TGTCGCCATC TGTGGAAAGA 
CCAGATTACA GGAACATCAC ATTTGATGAA CTTGTTGAAG CATACAAAGA GCAGGCCAAA 
GGGCTTCTGG ATGGTGGAGT GGATATCTTA CTCATTGAAA CTATTTTTGA TACCGCCAAT 
GCCAAGGCAG CCTTGTTTGC AGTCCAAAAA CTTTTTGAAG AGGAGTATGT TCCCCGGCCT 
GTCTTTATTT CAGGGACAAT TGTTGATAAA AGTGGGCGGA CTCTCTCTGG CCAGACTGGG 
GAGGCATTTG TCATCAGCGT GTCTCATGCA GACCCACTCT GCATTGGATT AAATTGTGCT 
CTGGGTGCAG CTGAAATGAG ACCTTTTATT GAAACAATTG GAAAATGTAC AACAGCTTAT 
GTTCTCTGTT ATCCCAATGC AGGTCTTCCC AATACCTTCG GTGACTATGA TGAAACTCCC 
CACGTGATGG CCATGCATTT AAAGGATTTT GCTGTTGATG GCTTGGTCAA CATAGTTGGC 
GGATGCTGTG GTACGACACC AGATCATATC AGAGAAATTG CTGAAGCTGT GAAAAACTGT 
AAGCCTAGAG TTCCACCTGC CACTGTTTTC GAAGGGCATA TGCTACTGTC TGGTCTAGAG 
CCCTTCAGGA TTGGACCATA CACCAACTTT GTTAACATTG GAGAGCGCTG TAACGTTGCA 
GGATCCAGGA GATTTGCTAA GCTCATCATG GCAGGAAATT ATGAAGAAGC ACTGAGCGTT 
GCCAAAATGC AGGTGGAAAT GGGAGCCCAG GTGTTGGATA TTAACATGGA TGATGGCATG 
CTGGATGGTC CAAGTGCAAT GACCAGATTC TGCAACTTCA TTGCTTCCGA GCCTGACATT 
GCCAAGGTGC CCTTGTGCAT TGACTCTTCC AATTTTGCTG TGATTGAAGC CGGCTTAAAG 
TGCTGCCAGG GGAAGTGCAT TGTTAATAGC ATCAGCTTGA AGGAGGGAGA GGATGACTTC 
TTGGAGAAGG CCAGAAAGAT TAAGAAGTTT GGAGCTGCCG TGGTGGTTAT GGCTTTTGAT 
GAAGAAGGAC AGGCAACAGA AACAGATACC AAAATCAGAG TGTGCACCCG GGCCTACCAT 
CTCCTTTTGA AAAAACTGGG CTTCAATCCA AATGACATCA TCTTTGACCC TAATATCCTC 
ACCATTGGTA CTGGGATGGA AGAACATAAC TTGTATGCTG TTAATTTTAT CAATGCAACA 
AAAGTCATTA AAGAAACGCT GCCTGGAGCC AAAGTAAGCG GTGGTCTTTC CAACTTGTCC 
TTCTCCTTCC GAGGAATGGA AGCCATTCGA GAGGCAATGC ATGGGGTTTT CCTGTACCAT 
GCGATCAAGT TTGGTATGGA CATGGGAATA GTGAATGCTG GAAGCCTTCC TGTATATGAT 
GACATCCACA AGGAGCTTCT CCAGCTCTGT GAGGACCTCA TCTGGAACAG AGACCCTGAG 
GCCACCGAGA AGCTCTTACA CTACGCCCAG ACTCAGGGCA AAGGAGGGAA GAAAGTCATC 
CAGACTGATG AGTGGAGGAA TGGCCCTCTC GAAGAACGCC TTGAGTACGC TCTTGTGAAG 
GGCATTGAAA AATATATTAT TGAAGATACT GAGGAAGCCA GGTTAAACCA GGAAAAATAT 
CCCCGACCTC TGAATATAAT TGAAGGGCCG CTGATGAATG GCATGAAAAT TGTTGGTGAT 
CTTTTTGGAG CTGGAAAAAT GTTTCTACCT CAGGTTATAA AGTCAGCTCG GGTCATGAAG 
AAGGCCGTGG GTCACCTTAT TCCCTTCATG GAAAAAGAGA GAGAAGAAAC CAAAGTGCTT 
ACTGGCAAAA TAGAAGATGA GGACCCTTAC CAAGGCACCA TCGTGCTGGC TACTGTTAAA 
GGTGACGTAC ACGACATAGG CAAGAACATA GTTGGAGTGG TTCTTGGCTG CAATAATTTC 
AGAGTTATTG ATTTAGGAGT CATGACTCCA TGTGACAAGA TACTGAAGGC TGCTCTAGAC 
CACAAAGCAG ATATAATTGG CCTGTCAGGA CTCATCACTC CTTCCCTGGA TGAAATGATT 
TTTGTCGCTA AGGAAATGGA GAGACTAGCT ATAAAGATCC CATTGTTGAT CGGAGGAGCT 
ACCACTTCCA GAACCCACAC AGCAGTTAAA ATAGCCCCAA GATACAGTGC GCCCGTGATC 
CACGTCCTGG ATGCATCCAA GAGCGTGGTG GTGTGTTCTC AACTGTTAGA TGAGAATCTA 
AAAGATGAGT ACTTTGAGGA GATCCTGGAA GAATATGAAG ATATTAGACA GGACCATTAT 
GAATCTCTCA AGGAGAGAAG ATACTTAACC TTAAGGCAAG CTAGAGAAAA TGGTTTCCAT 
ATTGACTGGC TGTCAGAGCC TCCTCCAGTG AAGCCCACGT TCCTCGGGAC TCGGGTCTTT 
GAAGACTACG ACCTGCAGAA GCTGGTGGAC TACATTGATT GGAAGCCTTT CTTCGACGTC 
TGGCAGCTCC GGGGCAAATA CCCGAACCGA GGCTTTCCCA AGATATTTGA CGACAAAACT 
GTAGGTGAAG AGGCCAAAAA GGTCTATGAT GATGCCCAAA ATATGCTGCA GGCACTGATC 
AGTCAGAAGA AGCTCCAGGC CAGGGGTGTG GTTGGGTTCT GGCCAGCACA GAGCATTCAG 
GATGATATCC ACCTGTACGC GGAAGGCGCA GTGCCACAGG CCTCGGAGCC CATAGCCACC 
TTCTACGGGC TCAGGCAGCA GGCCGAGAAG GACTCTGCCA GCTCGGACCC CTACCTCTGC 
CTCTCGGACT TCATCGCCCC TCTGCACTCA GGTATCCCCG ACTACCTGGG CCTGTTCGCC 
GTTGCCTGCT TTGGGGTGGA GGAGCTGAGC AAGGCCTACG AGGAGGAGTG TGATGACTAC 
AGCAGCATCA TGGTCAAGGC GCTGGGGGAC CGGCTGGCCG AGGCCTTCGC TGAGGAGCTG 
CACGAGCGGG TCCGCAGGGA GCTGTGGGGT TACTGTAGTG GTGAGCAGCT GGCTGTGGCT 
GACCTGCGGA GACTGCGCTA CGAGGGCATC CGGCCAGCGC CCGGCTATCC CAGCCAGCCT 
GACCACACTG AGAAGCTGAC CATGTGGAGG CTGGCGGACG TGGAGCAGCG CACAGGCATT 
AGGTTAACAG AATCGCTGGC CATGGCACCT GCTTCAGCAG TATCTGGCCT TTACTTCTCC 
AACTTGAAGT CCAAGTATTT TGCCGTGGGG AAAATTTCCA AGGATCAGAT TGAGGATTAT 
GCTTCGAGGA AGAACATGTC TGTGGCCGAG GTTGAGAAAT GGCTTGGACC CATTTTGGGA 
TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818713.1
CDS	450..4244
Translation	MAPTLQDLTPSGMKKTLQDEIEAILQERIMVLDGGMGTMIQQHKLSEEDFRGQEFKDHARPLKGNNDILSITQPSVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEDISLQTGIKRYVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYKEQAKGLLDGGVDILLIETIFDTANAKAALFAVQKLFEEEYVPRPVFISGTIVDKSGRTLSGQTGEAFVISVSHADPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPHVMAMHLKDFAVDGLVNIVGGCCGTTPDHIREIAEAVKNCKPRVPPATVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRRFAKLIMAGNYEEALSVAKMQVEMGAQVLDINMDDGMLDGPSAMTRFCNFIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKFGAAVVVMAFDEEGQATETDTKIRVCTRAYHLLLKKLGFNPNDIIFDPNILTIGTGMEEHNLYAVNFINATKVIKETLPGAKVSGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGSLPVYDDIHKELLQLCEDLIWNRDPEATEKLLHYAQTQGKGGKKVIQTDEWRNGPLEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETKVLTGKIEDEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEILEEYEDIRQDHYESLKERRYLTLRQARENGFHIDWLSEPPPVKPTFLGTRVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEAKKVYDDAQNMLQALISQKKLQARGVVGFWPAQSIQDDIHLYAEGAVPQASEPIATFYGLRQQAEKDSASSDPYLCLSDFIAPLHSGIPDYLGLFAVACFGVEELSKAYEEECDDYSSIMVKALGDRLAEAFAEELHERVRRELWGYCSGEQLAVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLADVEQRTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYASRKNMSVAEVEKWLGPILGYDTD

Target ORF information:

RefSeq Version	XM_010820411.3
Organism	Bos taurus(cattle)
Definition	Bos taurus 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820411.3

ORF Insert Sequence:

ATGGCCCCCA CGCTCCAGGA CCTGACGCCA TCTGGGATGA AGAAGACCCT GCAAGATGAG 
ATTGAAGCTA TCCTGCAGGA GAGGATTATG GTGCTTGATG GAGGGATGGG GACCATGATC 
CAGCAGCACA AGCTGAGTGA AGAAGACTTC CGAGGACAAG AATTTAAAGA TCATGCCAGG 
CCCCTGAAAG GCAACAATGA CATTTTAAGT ATAACTCAGC CCAGTGTCAT TTACCAAATC 
CATAAGGAGT ACCTCCTAGC TGGGGCAGAT ATCATTGAAA CAAATACTTT CAGCAGCACT 
TCTATTGCCC AAGCTGACTA TGGCCTGGAA CACTTGGCCT ACCGGATGAA CATGTGCTCT 
GCGGGAGTGG CCAGAAAAGC AGCCGAGGAC ATCTCTCTCC AGACAGGAAT TAAGAGGTAT 
GTGGCAGGAG CTCTGGGTCC AACTAACAAG ACACTCTCTG TGTCGCCATC TGTGGAAAGA 
CCAGATTACA GGAACATCAC ATTTGATGAA CTTGTTGAAG CATACAAAGA GCAGGCCAAA 
GGGCTTCTGG ATGGTGGAGT GGATATCTTA CTCATTGAAA CTATTTTTGA TACCGCCAAT 
GCCAAGGCAG CCTTGTTTGC AGTCCAAAAA CTTTTTGAAG AGGAGTATGT TCCCCGGCCT 
GTCTTTATTT CAGGGACAAT TGTTGATAAA AGTGGGCGGA CTCTCTCTGG CCAGACTGGG 
GAGGCATTTG TCATCAGCGT GTCTCATGCA GACCCACTCT GCATTGGATT AAATTGTGCT 
CTGGGTGCAG CTGAAATGAG ACCTTTTATT GAAACAATTG GAAAATGTAC AACAGCTTAT 
GTTCTCTGTT ATCCCAATGC AGGTCTTCCC AATACCTTCG GTGACTATGA TGAAACTCCC 
CACGTGATGG CCATGCATTT AAAGGATTTT GCTGTTGATG GCTTGGTCAA CATAGTTGGC 
GGATGCTGTG GTACGACACC AGATCATATC AGAGAAATTG CTGAAGCTGT GAAAAACTGT 
AAGCCTAGAG TTCCACCTGC CACTGTTTTC GAAGGGCATA TGCTACTGTC TGGTCTAGAG 
CCCTTCAGGA TTGGACCATA CACCAACTTT GTTAACATTG GAGAGCGCTG TAACGTTGCA 
GGATCCAGGA GATTTGCTAA GCTCATCATG GCAGGAAATT ATGAAGAAGC ACTGAGCGTT 
GCCAAAATGC AGGTGGAAAT GGGAGCCCAG GTGTTGGATA TTAACATGGA TGATGGCATG 
CTGGATGGTC CAAGTGCAAT GACCAGATTC TGCAACTTCA TTGCTTCCGA GCCTGACATT 
GCCAAGGTGC CCTTGTGCAT TGACTCTTCC AATTTTGCTG TGATTGAAGC CGGCTTAAAG 
TGCTGCCAGG GGAAGTGCAT TGTTAATAGC ATCAGCTTGA AGGAGGGAGA GGATGACTTC 
TTGGAGAAGG CCAGAAAGAT TAAGAAGTTT GGAGCTGCCG TGGTGGTTAT GGCTTTTGAT 
GAAGAAGGAC AGGCAACAGA AACAGATACC AAAATCAGAG TGTGCACCCG GGCCTACCAT 
CTCCTTTTGA AAAAACTGGG CTTCAATCCA AATGACATCA TCTTTGACCC TAATATCCTC 
ACCATTGGTA CTGGGATGGA AGAACATAAC TTGTATGCTG TTAATTTTAT CAATGCAACA 
AAAGTCATTA AAGAAACGCT GCCTGGAGCC AAAGTAAGCG GTGGTCTTTC CAACTTGTCC 
TTCTCCTTCC GAGGAATGGA AGCCATTCGA GAGGCAATGC ATGGGGTTTT CCTGTACCAT 
GCGATCAAGT TTGGTATGGA CATGGGAATA GTGAATGCTG GAAGCCTTCC TGTATATGAT 
GACATCCACA AGGAGCTTCT CCAGCTCTGT GAGGACCTCA TCTGGAACAG AGACCCTGAG 
GCCACCGAGA AGCTCTTACA CTACGCCCAG ACTCAGGGCA AAGGAGGGAA GAAAGTCATC 
CAGACTGATG AGTGGAGGAA TGGCCCTCTC GAAGAACGCC TTGAGTACGC TCTTGTGAAG 
GGCATTGAAA AATATATTAT TGAAGATACT GAGGAAGCCA GGTTAAACCA GGAAAAATAT 
CCCCGACCTC TGAATATAAT TGAAGGGCCG CTGATGAATG GCATGAAAAT TGTTGGTGAT 
CTTTTTGGAG CTGGAAAAAT GTTTCTACCT CAGGTTATAA AGTCAGCTCG GGTCATGAAG 
AAGGCCGTGG GTCACCTTAT TCCCTTCATG GAAAAAGAGA GAGAAGAAAC CAAAGTGCTT 
ACTGGCAAAA TAGAAGATGA GGACCCTTAC CAAGGCACCA TCGTGCTGGC TACTGTTAAA 
GGTGACGTAC ACGACATAGG CAAGAACATA GTTGGAGTGG TTCTTGGCTG CAATAATTTC 
AGAGTTATTG ATTTAGGAGT CATGACTCCA TGTGACAAGA TACTGAAGGC TGCTCTAGAC 
CACAAAGCAG ATATAATTGG CCTGTCAGGA CTCATCACTC CTTCCCTGGA TGAAATGATT 
TTTGTCGCTA AGGAAATGGA GAGACTAGCT ATAAAGATCC CATTGTTGAT CGGAGGAGCT 
ACCACTTCCA GAACCCACAC AGCAGTTAAA ATAGCCCCAA GATACAGTGC GCCCGTGATC 
CACGTCCTGG ATGCATCCAA GAGCGTGGTG GTGTGTTCTC AACTGTTAGA TGAGAATCTA 
AAAGATGAGT ACTTTGAGGA GATCCTGGAA GAATATGAAG ATATTAGACA GGACCATTAT 
GAATCTCTCA AGGAGAGAAG ATACTTAACC TTAAGGCAAG CTAGAGAAAA TGGTTTCCAT 
ATTGACTGGC TGTCAGAGCC TCCTCCAGTG AAGCCCACGT TCCTCGGGAC TCGGGTCTTT 
GAAGACTACG ACCTGCAGAA GCTGGTGGAC TACATTGATT GGAAGCCTTT CTTCGACGTC 
TGGCAGCTCC GGGGCAAATA CCCGAACCGA GGCTTTCCCA AGATATTTGA CGACAAAACT 
GTAGGTGAAG AGGCCAAAAA GGTCTATGAT GATGCCCAAA ATATGCTGCA GGCACTGATC 
AGTCAGAAGA AGCTCCAGGC CAGGGGTGTG GTTGGGTTCT GGCCAGCACA GAGCATTCAG 
GATGATATCC ACCTGTACGC GGAAGGCGCA GTGCCACAGG CCTCGGAGCC CATAGCCACC 
TTCTACGGGC TCAGGCAGCA GGCCGAGAAG GACTCTGCCA GCTCGGACCC CTACCTCTGC 
CTCTCGGACT TCATCGCCCC TCTGCACTCA GGTATCCCCG ACTACCTGGG CCTGTTCGCC 
GTTGCCTGCT TTGGGGTGGA GGAGCTGAGC AAGGCCTACG AGGAGGAGTG TGATGACTAC 
AGCAGCATCA TGGTCAAGGC GCTGGGGGAC CGGCTGGCCG AGGCCTTCGC TGAGGAGCTG 
CACGAGCGGG TCCGCAGGGA GCTGTGGGGT TACTGTAGTG GTGAGCAGCT GGCTGTGGCT 
GACCTGCGGA GACTGCGCTA CGAGGGCATC CGGCCAGCGC CCGGCTATCC CAGCCAGCCT 
GACCACACTG AGAAGCTGAC CATGTGGAGG CTGGCGGACG TGGAGCAGCG CACAGGCATT 
AGGTTAACAG AATCGCTGGC CATGGCACCT GCTTCAGCAG TATCTGGCCT TTACTTCTCC 
AACTTGAAGT CCAAGTATTT TGCCGTGGGG AAAATTTCCA AGGATCAGAT TGAGGATTAT 
GCTTCGAGGA AGAACATGTC TGTGGCCGAG GTTGAGAAAT GGCTTGGACC CATTTTGGGA 
TATGATACAG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatic Activity and Transcription of Betaine-Homocysteine Methyltransferase, Methionine Synthase, and Cystathionine Synthase in Periparturient Dairy Cows Are Altered to Different Extents by Supply of Methionine and Choline.
The Journal of nutrition147(1)11-19(2017 01)
Zhou Z,Garrow TA,Dong X,Luchini DN,Loor JJ

Effects of dietary supplements of folic acid and vitamin B12 on metabolism of dairy cows in early lactation.
Journal of dairy science90(7)3442-55(2007 Jul)
Graulet B,Matte JJ,Desrochers A,Doepel L,Palin MF,Girard CL

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MTR cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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