MTTP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTTP
Entrez Gene ID	280868
Full Name	microsomal triglyceride transfer protein
Synonyms	MTP
General protein information	Preferred Names microsomal triglyceride transfer protein Names microsomal triglyceride transfer protein large subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101834.1	NP_001095304.1	microsomal triglyceride transfer protein large subunit precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTTP	NP_001095304.1
Danio rerio (zebrafish)	mtp	NP_998135.1
Xenopus tropicalis (tropical clawed frog)	LOC100487453	XP_002934813.1
Pan troglodytes (chimpanzee)	MTTP	XP_001167562.1
Drosophila melanogaster (fruit fly)	Mtp	NP_610075.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010234	XP_319421.4
	MTTP	XP_001107709.1
Rattus norvegicus (Norway rat)	Mttp	NP_001101197.1
Gallus gallus (chicken)	MTTP	NP_001103254.1
Homo sapiens (human)	MTTP	NP_000244.2
Canis lupus familiaris (dog)	MTTP	XP_544995.3
Mus musculus (house mouse)	Mttp	NP_032668.2

Gene Ontology
Bibliography

Related articles in PubMed

Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro.
Liu L, Li X, Li Y, Guan Y, Song Y, Yin L, Chen H, Lei L, Liu J, Li X, Wang Z, Yang X, Liu G
Journal of dairy science97(3)1328-35(2014 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Transfer of cholesteryl esters and phospholipids as well as net deposition by microsomal triglyceride transfer protein.
Rava P, Athar H, Johnson C, Hussain MM
Journal of lipid research46(8)1779-85(2005 Aug)

Abundance of mRNA of apolipoprotein b100, apolipoprotein e, and microsomal triglyceride transfer protein in liver from periparturient dairy cows.
Bernabucci U, Ronchi B, Basiric? L, Pirazzi D, Rueca F, Lacetera N, Nardone A
Journal of dairy science87(9)2881-8(2004 Sep)

Cloning and gene defects in microsomal triglyceride transfer protein associated with abetalipoproteinaemia.
Sharp D, Blinderman L, Combs KA, Kienzle B, Ricci B, Wager-Smith K, Gil CM, Turck CW, Bouma ME, Rader DJ
Nature365(6441)65-9(1993 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nonesterified fatty acids significantly inhibit the expression of ApoB100, ApoE, MTP, and LDLR, thereby decreasing the synthesis and assembly of VLDL and inducing TG accumulation in bovine hepatocytes.
Title: Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro.

measurements of the transfer of phospholipids (PLs) and cholesteryl esters (CEs)to acceptor vesicles by purified MTP showed TAG transfer activity was the most robust, and CE and PL transfer activities were 60-71% and 5-13% of the TAG transfer activity
Title: Transfer of cholesteryl esters and phospholipids as well as net deposition by microsomal triglyceride transfer protein.

after calving the apolipoprotein B(100) mRNA synthesis was lower, whereas microsomal triglyceride transfer protein (MTP) and apolipoprotein E messenger RNA abundance were higher in the liver
Title: Abundance of mRNA of apolipoprotein b100, apolipoprotein e, and microsomal triglyceride transfer protein in liver from periparturient dairy cows.

The following MTTP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTTP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43255	NM_001101834.1 Latest version!	Bos taurus microsomal triglyceride transfer protein (MTTP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTTP ( NM_001101834.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095304.1 Bos taurus microsomal triglyceride transfer protein (MTTP), mRNA.

Price & Availability↑

CloneID	OBa43255
Clone ID Related Accession (Same CDS sequence)	NM_001101834.1
Accession Version	NM_001101834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Bos taurus(cattle)
Product	microsomal triglyceride transfer protein large subunit precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151485.1. On Aug 21, 2007 this sequence version replaced XM_592505.3. ##Evidence-Data-START## Transcript exon combination :: BC151485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145427, SAMN03145482 [ECO:0000348] ##Evidence-Data-END##

ATGATTCTTC TCGCTGTGCT TTTTCTCTGC TTCATTTCCT CATATTCAGC TTCTGTTAAA 
GGTCACACAA CTGGTCTCTC ATTAAATAAT GACCGACTAT ACAAACTCAC ATACTCCACT 
GAAGTTTTTC TCGATCGGGG CAAAGGAAAC CTCCAAGACA GTGTGGGCTA CCGAATTTCA 
TCCAATGTGG ATGTCGCTTT ACTGTGGAGG AGTCCTGATG GTGATGATAA CCAACTGATC 
CAAATTACGA TGAAAGATGT AAACCTTGAA AATGTGAATC AACAGAGAGG AGAGAAGAGC 
ATTTTCAAAG GAAAAAAGTC ATCTCAAATC ATAAGAAAGG AAAACTTGGA AGCAATGCAA 
AGACCTGTGC TCCTTCATCT AATTCATGGA AAGATCAAAG AGTTCTACTC ATATCAAAAT 
GAACCAGCAG CCATAGAAAA TCTCAAGAGA GGCCTGGCTA GCCTATTTCA GATGCAGTTA 
AGCTCTGGAA CTACCAATGA GGTAGACATC TCTGGAGATT GTAAAGTGAC CTACCAGGCT 
CATCAAGACA AAGTGACCAA AATTAAGGCT TTGGATTCAT GCAAAATAGA GAGGGCTGGA 
TTTACGACCC CACATCAGGT CTTGGGTGTC ACTTCGAAAG CCACATCTGT CACTACCTAT 
AAGATAGAAG ACAGCTTTGT TGTAGCTGTG CTCTCAGAAG AGATACGTGC TTTAAGGCTC 
AATTTTCTAC AATCAATAGC AGGCAAAATA GTATCGAGGC AGAAACTGGA GCTGAAAACC 
ACGGAAGCAA GCGTGAGACT GAAGCCAGGA AAGCAGGTTG CAGCCATCAT TAAAGCAGTC 
GATTCAAAGT ACACGGCCAT TCCCATTGTG GGGCAGGTCT TCCAGAGCAA GTGCAAAGGA 
TGCCCTTCTC TCTCAGAGCA CTGGCAGTCC ATCAGAAAAC ACCTGCAGCC TGACAACCTC 
TCCAAGGCTG AGGCTGTCAG AAGCTTCCTG GCCTTCATCA AGCACCTCAG GACGGCAAAG 
AAAGAAGAGA TCCTCCAAAT TCCAAAGGCA GAAAACAAGG AAGTACTACC CCAGCTAGTG 
GATGCTGTCA CCTCTGCTCA GACACCAGAC TCATTAGACG CCATTTTGGA CTTTCTGGAT 
TTCAAAAGCA CCGAGAGCGT TATCCTCCAG GAAAGGTTTC TCTATGCCTG TGCATTTGCC 
TCACATCCTG ATGAAGAACT CCTGAGAGCC CTCATTAGTA AGTTCAAAGG TTCTTTTGGA 
AGCAATGACA TCAGAGAATC TGTTATGATC ATCATCGGGG CCCTTGTCAG GAAGTTGTGT 
CAGAACCAAG GCTGCAAACT GAAAGGAGTA ATAGAAGCCA AAAAGTTAAT CTTGGGAGGA 
CTTGAAAAAG CAGAGAAAAA AGAGGACATC GTGATGTACC TGCTGGCTCT GAAGAACGCC 
CGGCTTCCAG AAGGCATCCC GCTCCTTCTG AAGTACACAG AGACAGGAGA AGGGCCCATT 
AGCCACCTTG CCGCCACCAC ACTCCAGAGA TATGATGTCC CTTTCATAAC TGATGAGGTA 
AAGAAGACTA TGAACAGGAT ATACCACCAG AATCGTAAAA TACATGAAAA AACTGTGCGT 
ACTACTGCAG CTGCCATCAT TTTAAAAAAC AATCCATCCT ACATGGAAGT AAAAAACATC 
CTGCTCTCTA TTGGGGAACT TCCCAAAGAA ATGAATAAGT ACATGCTCTC CATTGTCCAA 
GACATCCTAC GTTTTGAAAC ACCTGCAAGC AAAATGGTCC GTCAAGTTCT GAAGGAAATG 
GTCGCTCATA ATTACGATCG TTTCTCCAAG AGTGGGTCCT CCTCTGCATA TACTGGCTAC 
GTAGAACGGA CTTCCCATTC GGCATCTACT TACAGCCTTG ACATTCTTTA CTCTGGTTCT 
GGCATTCTAA GGAGAAGTAA TCTGAACATC TTTCAGTATA TTGAGAAAAC TCCTCTTCAT 
GTCATCCAGG TGGTCATTGA AGCCCAAGGA CTGGAGGCAT TAATTGCAGC CACTCCTGAT 
GAGGGGGAAG AGAACCTTGA CTCCTATGCT GGCTTGTCAG CTCTCCTCTT TGATGTTCAG 
CTCAGACCTG TCACTTTTTT CAACGGGTAC AGTGATTTGA TGTCCAAAAT GCTGTCAGCA 
TCTAGTGACC CTATGAGTGT GGTGAAAGGA CTTCTTCTGC TAATAGATCA TTCCCAGGAG 
CTTCAGCTGC AATCTGGACT TAAGGCCAAT ATGGATGTTC AAGGTGGTCT AGCTATTGAT 
ATTACAGGTG CCATGGAGTT TAGTCTATGG TATCGTGAAT CTAAAACCCG AGTGAAAAAT 
CGGGTAAGTG TGTTAATAAC TGGTGGCATC ACGGTGGACT CCTCTTTTGT GAAAGCTGGC 
TTGGAAATTG GTGCAGAAAC AGAAGCAGGC TTGGAGTTTA TCTCCACGGT GCAGTTTTCT 
CAGTACCCAT TTTTAGTTTG TCTGCAGATG GACAAGGAAG ATGTTCCATA CAGGCAGTTT 
GAGACAAAAT ATGAAAGGCT GTCCACAGGC AGAGGTTACA TCTCTCGGAA GAGAAAAGAA 
AGCCTAATAG GAGGATGTGA ATTCCCGCTG CACCAAGAGA ACTCTGACAT GTGCAAGGTG 
GTGTTTGCTC CTCAACCAGA GAGCAGTTCC AGTGGTTGGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095304.1
CDS	85..2769
Translation	MILLAVLFLCFISSYSASVKGHTTGLSLNNDRLYKLTYSTEVFLDRGKGNLQDSVGYRISSNVDVALLWRSPDGDDNQLIQITMKDVNLENVNQQRGEKSIFKGKKSSQIIRKENLEAMQRPVLLHLIHGKIKEFYSYQNEPAAIENLKRGLASLFQMQLSSGTTNEVDISGDCKVTYQAHQDKVTKIKALDSCKIERAGFTTPHQVLGVTSKATSVTTYKIEDSFVVAVLSEEIRALRLNFLQSIAGKIVSRQKLELKTTEASVRLKPGKQVAAIIKAVDSKYTAIPIVGQVFQSKCKGCPSLSEHWQSIRKHLQPDNLSKAEAVRSFLAFIKHLRTAKKEEILQIPKAENKEVLPQLVDAVTSAQTPDSLDAILDFLDFKSTESVILQERFLYACAFASHPDEELLRALISKFKGSFGSNDIRESVMIIIGALVRKLCQNQGCKLKGVIEAKKLILGGLEKAEKKEDIVMYLLALKNARLPEGIPLLLKYTETGEGPISHLAATTLQRYDVPFITDEVKKTMNRIYHQNRKIHEKTVRTTAAAIILKNNPSYMEVKNILLSIGELPKEMNKYMLSIVQDILRFETPASKMVRQVLKEMVAHNYDRFSKSGSSSAYTGYVERTSHSASTYSLDILYSGSGILRRSNLNIFQYIEKTPLHVIQVVIEAQGLEALIAATPDEGEENLDSYAGLSALLFDVQLRPVTFFNGYSDLMSKMLSASSDPMSVVKGLLLLIDHSQELQLQSGLKANMDVQGGLAIDITGAMEFSLWYRESKTRVKNRVSVLITGGITVDSSFVKAGLEIGAETEAGLEFISTVQFSQYPFLVCLQMDKEDVPYRQFETKYERLSTGRGYISRKRKESLIGGCEFPLHQENSDMCKVVFAPQPESSSSGWF

Target ORF information:

RefSeq Version	NM_001101834.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microsomal triglyceride transfer protein (MTTP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101834.1

ORF Insert Sequence:

ATGATTCTTC TCGCTGTGCT TTTTCTCTGC TTCATTTCCT CATATTCAGC TTCTGTTAAA 
GGTCACACAA CTGGTCTCTC ATTAAATAAT GACCGACTAT ACAAACTCAC ATACTCCACT 
GAAGTTTTTC TCGATCGGGG CAAAGGAAAC CTCCAAGACA GTGTGGGCTA CCGAATTTCA 
TCCAATGTGG ATGTCGCTTT ACTGTGGAGG AGTCCTGATG GTGATGATAA CCAACTGATC 
CAAATTACGA TGAAAGATGT AAACCTTGAA AATGTGAATC AACAGAGAGG AGAGAAGAGC 
ATTTTCAAAG GAAAAAAGTC ATCTCAAATC ATAAGAAAGG AAAACTTGGA AGCAATGCAA 
AGACCTGTGC TCCTTCATCT AATTCATGGA AAGATCAAAG AGTTCTACTC ATATCAAAAT 
GAACCAGCAG CCATAGAAAA TCTCAAGAGA GGCCTGGCTA GCCTATTTCA GATGCAGTTA 
AGCTCTGGAA CTACCAATGA GGTAGACATC TCTGGAGATT GTAAAGTGAC CTACCAGGCT 
CATCAAGACA AAGTGACCAA AATTAAGGCT TTGGATTCAT GCAAAATAGA GAGGGCTGGA 
TTTACGACCC CACATCAGGT CTTGGGTGTC ACTTCGAAAG CCACATCTGT CACTACCTAT 
AAGATAGAAG ACAGCTTTGT TGTAGCTGTG CTCTCAGAAG AGATACGTGC TTTAAGGCTC 
AATTTTCTAC AATCAATAGC AGGCAAAATA GTATCGAGGC AGAAACTGGA GCTGAAAACC 
ACGGAAGCAA GCGTGAGACT GAAGCCAGGA AAGCAGGTTG CAGCCATCAT TAAAGCAGTC 
GATTCAAAGT ACACGGCCAT TCCCATTGTG GGGCAGGTCT TCCAGAGCAA GTGCAAAGGA 
TGCCCTTCTC TCTCAGAGCA CTGGCAGTCC ATCAGAAAAC ACCTGCAGCC TGACAACCTC 
TCCAAGGCTG AGGCTGTCAG AAGCTTCCTG GCCTTCATCA AGCACCTCAG GACGGCAAAG 
AAAGAAGAGA TCCTCCAAAT TCCAAAGGCA GAAAACAAGG AAGTACTACC CCAGCTAGTG 
GATGCTGTCA CCTCTGCTCA GACACCAGAC TCATTAGACG CCATTTTGGA CTTTCTGGAT 
TTCAAAAGCA CCGAGAGCGT TATCCTCCAG GAAAGGTTTC TCTATGCCTG TGCATTTGCC 
TCACATCCTG ATGAAGAACT CCTGAGAGCC CTCATTAGTA AGTTCAAAGG TTCTTTTGGA 
AGCAATGACA TCAGAGAATC TGTTATGATC ATCATCGGGG CCCTTGTCAG GAAGTTGTGT 
CAGAACCAAG GCTGCAAACT GAAAGGAGTA ATAGAAGCCA AAAAGTTAAT CTTGGGAGGA 
CTTGAAAAAG CAGAGAAAAA AGAGGACATC GTGATGTACC TGCTGGCTCT GAAGAACGCC 
CGGCTTCCAG AAGGCATCCC GCTCCTTCTG AAGTACACAG AGACAGGAGA AGGGCCCATT 
AGCCACCTTG CCGCCACCAC ACTCCAGAGA TATGATGTCC CTTTCATAAC TGATGAGGTA 
AAGAAGACTA TGAACAGGAT ATACCACCAG AATCGTAAAA TACATGAAAA AACTGTGCGT 
ACTACTGCAG CTGCCATCAT TTTAAAAAAC AATCCATCCT ACATGGAAGT AAAAAACATC 
CTGCTCTCTA TTGGGGAACT TCCCAAAGAA ATGAATAAGT ACATGCTCTC CATTGTCCAA 
GACATCCTAC GTTTTGAAAC ACCTGCAAGC AAAATGGTCC GTCAAGTTCT GAAGGAAATG 
GTCGCTCATA ATTACGATCG TTTCTCCAAG AGTGGGTCCT CCTCTGCATA TACTGGCTAC 
GTAGAACGGA CTTCCCATTC GGCATCTACT TACAGCCTTG ACATTCTTTA CTCTGGTTCT 
GGCATTCTAA GGAGAAGTAA TCTGAACATC TTTCAGTATA TTGAGAAAAC TCCTCTTCAT 
GTCATCCAGG TGGTCATTGA AGCCCAAGGA CTGGAGGCAT TAATTGCAGC CACTCCTGAT 
GAGGGGGAAG AGAACCTTGA CTCCTATGCT GGCTTGTCAG CTCTCCTCTT TGATGTTCAG 
CTCAGACCTG TCACTTTTTT CAACGGGTAC AGTGATTTGA TGTCCAAAAT GCTGTCAGCA 
TCTAGTGACC CTATGAGTGT GGTGAAAGGA CTTCTTCTGC TAATAGATCA TTCCCAGGAG 
CTTCAGCTGC AATCTGGACT TAAGGCCAAT ATGGATGTTC AAGGTGGTCT AGCTATTGAT 
ATTACAGGTG CCATGGAGTT TAGTCTATGG TATCGTGAAT CTAAAACCCG AGTGAAAAAT 
CGGGTAAGTG TGTTAATAAC TGGTGGCATC ACGGTGGACT CCTCTTTTGT GAAAGCTGGC 
TTGGAAATTG GTGCAGAAAC AGAAGCAGGC TTGGAGTTTA TCTCCACGGT GCAGTTTTCT 
CAGTACCCAT TTTTAGTTTG TCTGCAGATG GACAAGGAAG ATGTTCCATA CAGGCAGTTT 
GAGACAAAAT ATGAAAGGCT GTCCACAGGC AGAGGTTACA TCTCTCGGAA GAGAAAAGAA 
AGCCTAATAG GAGGATGTGA ATTCCCGCTG CACCAAGAGA ACTCTGACAT GTGCAAGGTG 
GTGTTTGCTC CTCAACCAGA GAGCAGTTCC AGTGGTTGGT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro.
Journal of dairy science97(3)1328-35(2014 Mar)
Liu L,Li X,Li Y,Guan Y,Song Y,Yin L,Chen H,Lei L,Liu J,Li X,Wang Z,Yang X,Liu G

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Transfer of cholesteryl esters and phospholipids as well as net deposition by microsomal triglyceride transfer protein.
Journal of lipid research46(8)1779-85(2005 Aug)
Rava P,Athar H,Johnson C,Hussain MM

Abundance of mRNA of apolipoprotein b100, apolipoprotein e, and microsomal triglyceride transfer protein in liver from periparturient dairy cows.
Journal of dairy science87(9)2881-8(2004 Sep)
Bernabucci U,Ronchi B,Basiric? L,Pirazzi D,Rueca F,Lacetera N,Nardone A

Cloning and gene defects in microsomal triglyceride transfer protein associated with abetalipoproteinaemia.
Nature365(6441)65-9(1993 Sep)
Sharp D,Blinderman L,Combs KA,Kienzle B,Ricci B,Wager-Smith K,Gil CM,Turck CW,Bouma ME,Rader DJ

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MTTP cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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