PRG4 cDNA ORF clone, Bos taurus(cattle)

The following PRG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223399 XM_024975994.1
Latest version!
Bos taurus proteoglycan 4 (PRG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223399 XM_024975995.1
Latest version!
Bos taurus proteoglycan 4 (PRG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223400 XM_024975996.1
Latest version!
Bos taurus proteoglycan 4 (PRG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223401 XM_024975998.1
Latest version!
Bos taurus proteoglycan 4 (PRG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa41101 NM_001206633.1
Latest version!
Bos taurus proteoglycan 4 (PRG4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223399
    Clone ID Related Accession (Same CDS sequence) XM_024975994.1 , XM_024975995.1
    Accession Version XM_024975994.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product proteoglycan 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTAAA AGATAAGAAG AAAAGAACTC CTAAAAATAA ACCTACCCCA
    GAACCACCAG TTATAGATGA AGATGGAAGT GGACTGGATA ATGGTGATTT CAAGCTCACC
    CCAACTCCTA ACATTCCCAC CACACAACGT AATAAAGTGA CCACAACTCC CAAGATTACA
    ACAGTAAAAC CAACACTTCC TAAGCCCAGT CTTCCACCTA ATTCTGATAC AACTAAAGAG
    ATACCTTCGA CAGTCAACAG AGAGACAACA GTTGAAACTA AAGAGACTTC TACAACAAAT
    AAACCGACTT CCACTAGCGC AAAAGAGAAG ACAACTTCAG CTAAAGAGAC ACGAACTGCA
    GAGAATACAT CTACTAAAGG TCTTGCACCC ACACCTGAAG TTCCTGCTAT ATCTACACCC
    AAAGCTGAAA CTATAACCAG ATCCCCTACA CCCACCACCC CCAAGGAGCC TGCTCCCACC
    ACCAAGGAGC CTGCTCTCAC CACCACCACC AAGGAGCCTG CTTCCACCAC CCCAAAGAAG
    CCTGCACCCA CCACCCCTAA GGAGACTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    AAGGAGCCTG AACCCACCAC CCCCAAGCAG CCTGCTCCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCC CTAAGGAGAC TGCACCCACC ACCAAGGAGC CTGAACCCAC CACCCCCAAG
    CAGCCTGCTC CCACCACCCC CAAGCAGCCT GCACCCACCA CCTCTAAGGA GACTGCACCC
    ACCACCAAGG AGCCTGCTCC CACCACCCCC AAGGAGCCTG CTCCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GGAGCCTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    CCCAAGCAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCAC CCACCACCCC TAAGGAGACT
    GCACCCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCACCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCA AGGAGCCTGA ATCCACCACC AAGGAGCCTG AACCCACCAC CAAGGAGCCT
    GAACCCACCA CCCCCAAGCA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC ACCCACCACC
    CCCAAGGAGA CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACCCCCAA GCAGCCTGCT
    CCCACCACCC CCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCACC CACCACCCCC
    AAGGAGCCTG CACCCACCAC CAAGGAGCCT GCACCCACCA CCAAGGAGCC TGAACCCACC
    ACCAAGGAGC CTGCACCCAC TACCACCCCC AAGGAGCCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCCTCA AGCAGCCTGC TCCCACCACC
    CCCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCTC CCACCACCCC CAAGGAGCCT
    ACTCCCACCA CCCCCAAGGA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC TCCCACCACC
    CCCAAGGAGC CTACTCCCAC TACTCCCAAG GAGCCTGCTC CCACCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCACCCAC CATCCCCAAG GAACCTGCAC CCACCACCCC CAAAGAGCCT
    GCTCCCACCA CCCACAAGGA GCCTACACCC ACCACCACCC CCAAAGAGCC TGCTCCCACC
    AGCCCCAAGG AACCTGCTCC CACCAGCCCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCCC CAAGGAGCCT GCTCCCACCG ATCCCAAGGA GCCTGCTCCC
    GCTGAACCCA AGGAGCCTGC ACCCACCAGC TCCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCTC CGACCGATCC CAAGGAGCCT
    GCTCCCGCTG AACCCAAGGA GCCTGCACCC ACCAGCCCCA AGGAGCCTGC ACCCACTACC
    ACCACCAAGG AGCCTGCTCC CGCTGAACCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCAC CAAGGAGCCT GCTCCCGCTG AACCCAAGGA GCCTGCACCC
    ACCAGCCCCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCTCC CACTACCACC
    CCCAAGGAGC CTGCTCCGAC CGATCCCAAG GAGCCTGCTC CCGCTGAACC CAAGGAGCCA
    GCTCCTGCTG AACCCAAGGA GCCCACTCCA AATTCTCCTG AGACACTTGC TCCAAGTTCT
    CCTGATATCC CTGCTCCAAC CACCTCAGAG GCCTCTACTT CAACTACCAC CGTGGAGCCT
    TCCACTACTC TGAAGAGCCC TGCTGAATCA ACTCCTCAAC CTCCTATAGA ACCCACACCA
    AAGGCTCTTG AAAACAGTCC CAAGGAACCT GCAGTACCCA CAATCAAGGG TCCTAAAGTG
    AACAAACCTG AAATAACTAC AACAGTTAAA GACAAGTCCA CAGAAAAAGA CATATATTCT
    ACACCTGAAA TTACAACAGC TGTAGCTAAG ATTATAACAG AGGCAGCAAC TAAAACAGAA
    AAGACTACTG AATCGAAAAT ACCAAGTACA ACCACAGATG TAACATCTAC CACAACCAAA
    GATACCACGT CATCCAAAAT TACTCCTAAA GCAACTCTTG CACCTAAAGT AATGACTGCA
    ACAAAGAAGA CGACCGAAAA GACTAAGAAC AAACCTGAAA AAACCACAGC TGTACCAAAA
    GGCAGAGCTA CTAATTCTCA AGTGACAACT CCTAAACCGC AGAAACCAAC CAAAGCACCC
    AAAAAGCCCA CTTCGACCAA AAAGCCAAGA ACACCTAGAG TGAGAAAACC AAAGACTACG
    CCAACGCCCC CAAAGACGAC GACATCAGCA ATGCCTGAAC CGACTCCTAC CTCTTTACCA
    GAAGCCATGC TCCAAACCAC CACCAGGCCT ACCCCAACCC CCAACTCAGA AATAATTGAC
    GTAAACCCAG AGAATGAGGA TGGAGATGCT GCTGAAGGAG AAAAACCTCA CATGATTTTC
    AGGCCCCCTG TGCTAACTCC TATAGTGATT CCAGGCACTG AAATCATAGT GAGAGGACCC
    AGTCAAGGCT TCGGCATCAA CCCCATGTTT TCAGATGAAA CTAATCTATG CAACGGTAGG
    CCAGTAGATG GACTGACTAC TTTGCGTAAT GGGACATTAG TTGCATTTCG AGGTCATTAT
    TTCTGGATGC TGACTCCATT TACTCCACCA CCTCCACCTC GGAGAATTAC TGAAGTTTGG
    GGCATTCCCT CCCCCATTGA TACTGTTTTT ACTAGATGCA ACTGTGAAGG AAAAACTTTC
    TTCTTTAAGG GTTCTCAGTA CTGGCGTTTC ACCAATGATA TAAAAGATGC AGGATATCCC
    AAACTAATTT CCAAAGGATT TGGAGGACTG AATGGAAAAA TAGTGGCAGC CCTCTCAATA
    GCTCAGTACA AGAGCAGACC TGAATCCGTG TATTTTTTCA AGAGAGGTGG CAGCGTTCAG
    CAATACACTT ACAAACAGGA ACCCACCCAA AAGTGTACTG GAAGAAGGCC TGCGATAAAC
    TATTCTGTGT ATGGAGAAAC AGCACAGGTT AGGAGACGTC GATTTGAACG TGCTATAGGA
    CCTTCTCAAG TACACACCAT CAGGATTCAC TACACACCCG TCAGAGTCCC TTATCAAGAC
    AAAGGTTTCC TCCATAATGA AGTTAAAGTG AGTACACTGT GGAGAGGACT TCCAAATGTG
    GTTACTTCAG CAATATCACT GCCCAACATC AGAAAACCCG ATGGCTATGA TTACTATGCC
    CTTTCTAAAG ATCAATACTA TAACATCGAC GTGCCAAGTA GGACAGCAAG AGCCATTACT
    ACTCGTTCTG GGCAGACCTT ATCCAATACC TGGTACAACT GTCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831762.1
    CDS536..4963
    Translation

    Target ORF information:

    RefSeq Version XM_024975994.1
    Organism Bos taurus(cattle)
    Definition Bos taurus proteoglycan 4 (PRG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTAAA AGATAAGAAG AAAAGAACTC CTAAAAATAA ACCTACCCCA
    GAACCACCAG TTATAGATGA AGATGGAAGT GGACTGGATA ATGGTGATTT CAAGCTCACC
    CCAACTCCTA ACATTCCCAC CACACAACGT AATAAAGTGA CCACAACTCC CAAGATTACA
    ACAGTAAAAC CAACACTTCC TAAGCCCAGT CTTCCACCTA ATTCTGATAC AACTAAAGAG
    ATACCTTCGA CAGTCAACAG AGAGACAACA GTTGAAACTA AAGAGACTTC TACAACAAAT
    AAACCGACTT CCACTAGCGC AAAAGAGAAG ACAACTTCAG CTAAAGAGAC ACGAACTGCA
    GAGAATACAT CTACTAAAGG TCTTGCACCC ACACCTGAAG TTCCTGCTAT ATCTACACCC
    AAAGCTGAAA CTATAACCAG ATCCCCTACA CCCACCACCC CCAAGGAGCC TGCTCCCACC
    ACCAAGGAGC CTGCTCTCAC CACCACCACC AAGGAGCCTG CTTCCACCAC CCCAAAGAAG
    CCTGCACCCA CCACCCCTAA GGAGACTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    AAGGAGCCTG AACCCACCAC CCCCAAGCAG CCTGCTCCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCC CTAAGGAGAC TGCACCCACC ACCAAGGAGC CTGAACCCAC CACCCCCAAG
    CAGCCTGCTC CCACCACCCC CAAGCAGCCT GCACCCACCA CCTCTAAGGA GACTGCACCC
    ACCACCAAGG AGCCTGCTCC CACCACCCCC AAGGAGCCTG CTCCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GGAGCCTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    CCCAAGCAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCAC CCACCACCCC TAAGGAGACT
    GCACCCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCACCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCA AGGAGCCTGA ATCCACCACC AAGGAGCCTG AACCCACCAC CAAGGAGCCT
    GAACCCACCA CCCCCAAGCA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC ACCCACCACC
    CCCAAGGAGA CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACCCCCAA GCAGCCTGCT
    CCCACCACCC CCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCACC CACCACCCCC
    AAGGAGCCTG CACCCACCAC CAAGGAGCCT GCACCCACCA CCAAGGAGCC TGAACCCACC
    ACCAAGGAGC CTGCACCCAC TACCACCCCC AAGGAGCCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCCTCA AGCAGCCTGC TCCCACCACC
    CCCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCTC CCACCACCCC CAAGGAGCCT
    ACTCCCACCA CCCCCAAGGA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC TCCCACCACC
    CCCAAGGAGC CTACTCCCAC TACTCCCAAG GAGCCTGCTC CCACCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCACCCAC CATCCCCAAG GAACCTGCAC CCACCACCCC CAAAGAGCCT
    GCTCCCACCA CCCACAAGGA GCCTACACCC ACCACCACCC CCAAAGAGCC TGCTCCCACC
    AGCCCCAAGG AACCTGCTCC CACCAGCCCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCCC CAAGGAGCCT GCTCCCACCG ATCCCAAGGA GCCTGCTCCC
    GCTGAACCCA AGGAGCCTGC ACCCACCAGC TCCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCTC CGACCGATCC CAAGGAGCCT
    GCTCCCGCTG AACCCAAGGA GCCTGCACCC ACCAGCCCCA AGGAGCCTGC ACCCACTACC
    ACCACCAAGG AGCCTGCTCC CGCTGAACCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCAC CAAGGAGCCT GCTCCCGCTG AACCCAAGGA GCCTGCACCC
    ACCAGCCCCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCTCC CACTACCACC
    CCCAAGGAGC CTGCTCCGAC CGATCCCAAG GAGCCTGCTC CCGCTGAACC CAAGGAGCCA
    GCTCCTGCTG AACCCAAGGA GCCCACTCCA AATTCTCCTG AGACACTTGC TCCAAGTTCT
    CCTGATATCC CTGCTCCAAC CACCTCAGAG GCCTCTACTT CAACTACCAC CGTGGAGCCT
    TCCACTACTC TGAAGAGCCC TGCTGAATCA ACTCCTCAAC CTCCTATAGA ACCCACACCA
    AAGGCTCTTG AAAACAGTCC CAAGGAACCT GCAGTACCCA CAATCAAGGG TCCTAAAGTG
    AACAAACCTG AAATAACTAC AACAGTTAAA GACAAGTCCA CAGAAAAAGA CATATATTCT
    ACACCTGAAA TTACAACAGC TGTAGCTAAG ATTATAACAG AGGCAGCAAC TAAAACAGAA
    AAGACTACTG AATCGAAAAT ACCAAGTACA ACCACAGATG TAACATCTAC CACAACCAAA
    GATACCACGT CATCCAAAAT TACTCCTAAA GCAACTCTTG CACCTAAAGT AATGACTGCA
    ACAAAGAAGA CGACCGAAAA GACTAAGAAC AAACCTGAAA AAACCACAGC TGTACCAAAA
    GGCAGAGCTA CTAATTCTCA AGTGACAACT CCTAAACCGC AGAAACCAAC CAAAGCACCC
    AAAAAGCCCA CTTCGACCAA AAAGCCAAGA ACACCTAGAG TGAGAAAACC AAAGACTACG
    CCAACGCCCC CAAAGACGAC GACATCAGCA ATGCCTGAAC CGACTCCTAC CTCTTTACCA
    GAAGCCATGC TCCAAACCAC CACCAGGCCT ACCCCAACCC CCAACTCAGA AATAATTGAC
    GTAAACCCAG AGAATGAGGA TGGAGATGCT GCTGAAGGAG AAAAACCTCA CATGATTTTC
    AGGCCCCCTG TGCTAACTCC TATAGTGATT CCAGGCACTG AAATCATAGT GAGAGGACCC
    AGTCAAGGCT TCGGCATCAA CCCCATGTTT TCAGATGAAA CTAATCTATG CAACGGTAGG
    CCAGTAGATG GACTGACTAC TTTGCGTAAT GGGACATTAG TTGCATTTCG AGGTCATTAT
    TTCTGGATGC TGACTCCATT TACTCCACCA CCTCCACCTC GGAGAATTAC TGAAGTTTGG
    GGCATTCCCT CCCCCATTGA TACTGTTTTT ACTAGATGCA ACTGTGAAGG AAAAACTTTC
    TTCTTTAAGG GTTCTCAGTA CTGGCGTTTC ACCAATGATA TAAAAGATGC AGGATATCCC
    AAACTAATTT CCAAAGGATT TGGAGGACTG AATGGAAAAA TAGTGGCAGC CCTCTCAATA
    GCTCAGTACA AGAGCAGACC TGAATCCGTG TATTTTTTCA AGAGAGGTGG CAGCGTTCAG
    CAATACACTT ACAAACAGGA ACCCACCCAA AAGTGTACTG GAAGAAGGCC TGCGATAAAC
    TATTCTGTGT ATGGAGAAAC AGCACAGGTT AGGAGACGTC GATTTGAACG TGCTATAGGA
    CCTTCTCAAG TACACACCAT CAGGATTCAC TACACACCCG TCAGAGTCCC TTATCAAGAC
    AAAGGTTTCC TCCATAATGA AGTTAAAGTG AGTACACTGT GGAGAGGACT TCCAAATGTG
    GTTACTTCAG CAATATCACT GCCCAACATC AGAAAACCCG ATGGCTATGA TTACTATGCC
    CTTTCTAAAG ATCAATACTA TAACATCGAC GTGCCAAGTA GGACAGCAAG AGCCATTACT
    ACTCGTTCTG GGCAGACCTT ATCCAATACC TGGTACAACT GTCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223399
    Clone ID Related Accession (Same CDS sequence) XM_024975994.1 , XM_024975995.1
    Accession Version XM_024975995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product proteoglycan 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTAAA AGATAAGAAG AAAAGAACTC CTAAAAATAA ACCTACCCCA
    GAACCACCAG TTATAGATGA AGATGGAAGT GGACTGGATA ATGGTGATTT CAAGCTCACC
    CCAACTCCTA ACATTCCCAC CACACAACGT AATAAAGTGA CCACAACTCC CAAGATTACA
    ACAGTAAAAC CAACACTTCC TAAGCCCAGT CTTCCACCTA ATTCTGATAC AACTAAAGAG
    ATACCTTCGA CAGTCAACAG AGAGACAACA GTTGAAACTA AAGAGACTTC TACAACAAAT
    AAACCGACTT CCACTAGCGC AAAAGAGAAG ACAACTTCAG CTAAAGAGAC ACGAACTGCA
    GAGAATACAT CTACTAAAGG TCTTGCACCC ACACCTGAAG TTCCTGCTAT ATCTACACCC
    AAAGCTGAAA CTATAACCAG ATCCCCTACA CCCACCACCC CCAAGGAGCC TGCTCCCACC
    ACCAAGGAGC CTGCTCTCAC CACCACCACC AAGGAGCCTG CTTCCACCAC CCCAAAGAAG
    CCTGCACCCA CCACCCCTAA GGAGACTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    AAGGAGCCTG AACCCACCAC CCCCAAGCAG CCTGCTCCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCC CTAAGGAGAC TGCACCCACC ACCAAGGAGC CTGAACCCAC CACCCCCAAG
    CAGCCTGCTC CCACCACCCC CAAGCAGCCT GCACCCACCA CCTCTAAGGA GACTGCACCC
    ACCACCAAGG AGCCTGCTCC CACCACCCCC AAGGAGCCTG CTCCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GGAGCCTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    CCCAAGCAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCAC CCACCACCCC TAAGGAGACT
    GCACCCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCACCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCA AGGAGCCTGA ATCCACCACC AAGGAGCCTG AACCCACCAC CAAGGAGCCT
    GAACCCACCA CCCCCAAGCA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC ACCCACCACC
    CCCAAGGAGA CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACCCCCAA GCAGCCTGCT
    CCCACCACCC CCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCACC CACCACCCCC
    AAGGAGCCTG CACCCACCAC CAAGGAGCCT GCACCCACCA CCAAGGAGCC TGAACCCACC
    ACCAAGGAGC CTGCACCCAC TACCACCCCC AAGGAGCCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCCTCA AGCAGCCTGC TCCCACCACC
    CCCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCTC CCACCACCCC CAAGGAGCCT
    ACTCCCACCA CCCCCAAGGA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC TCCCACCACC
    CCCAAGGAGC CTACTCCCAC TACTCCCAAG GAGCCTGCTC CCACCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCACCCAC CATCCCCAAG GAACCTGCAC CCACCACCCC CAAAGAGCCT
    GCTCCCACCA CCCACAAGGA GCCTACACCC ACCACCACCC CCAAAGAGCC TGCTCCCACC
    AGCCCCAAGG AACCTGCTCC CACCAGCCCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCCC CAAGGAGCCT GCTCCCACCG ATCCCAAGGA GCCTGCTCCC
    GCTGAACCCA AGGAGCCTGC ACCCACCAGC TCCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCTC CGACCGATCC CAAGGAGCCT
    GCTCCCGCTG AACCCAAGGA GCCTGCACCC ACCAGCCCCA AGGAGCCTGC ACCCACTACC
    ACCACCAAGG AGCCTGCTCC CGCTGAACCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCAC CAAGGAGCCT GCTCCCGCTG AACCCAAGGA GCCTGCACCC
    ACCAGCCCCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCTCC CACTACCACC
    CCCAAGGAGC CTGCTCCGAC CGATCCCAAG GAGCCTGCTC CCGCTGAACC CAAGGAGCCA
    GCTCCTGCTG AACCCAAGGA GCCCACTCCA AATTCTCCTG AGACACTTGC TCCAAGTTCT
    CCTGATATCC CTGCTCCAAC CACCTCAGAG GCCTCTACTT CAACTACCAC CGTGGAGCCT
    TCCACTACTC TGAAGAGCCC TGCTGAATCA ACTCCTCAAC CTCCTATAGA ACCCACACCA
    AAGGCTCTTG AAAACAGTCC CAAGGAACCT GCAGTACCCA CAATCAAGGG TCCTAAAGTG
    AACAAACCTG AAATAACTAC AACAGTTAAA GACAAGTCCA CAGAAAAAGA CATATATTCT
    ACACCTGAAA TTACAACAGC TGTAGCTAAG ATTATAACAG AGGCAGCAAC TAAAACAGAA
    AAGACTACTG AATCGAAAAT ACCAAGTACA ACCACAGATG TAACATCTAC CACAACCAAA
    GATACCACGT CATCCAAAAT TACTCCTAAA GCAACTCTTG CACCTAAAGT AATGACTGCA
    ACAAAGAAGA CGACCGAAAA GACTAAGAAC AAACCTGAAA AAACCACAGC TGTACCAAAA
    GGCAGAGCTA CTAATTCTCA AGTGACAACT CCTAAACCGC AGAAACCAAC CAAAGCACCC
    AAAAAGCCCA CTTCGACCAA AAAGCCAAGA ACACCTAGAG TGAGAAAACC AAAGACTACG
    CCAACGCCCC CAAAGACGAC GACATCAGCA ATGCCTGAAC CGACTCCTAC CTCTTTACCA
    GAAGCCATGC TCCAAACCAC CACCAGGCCT ACCCCAACCC CCAACTCAGA AATAATTGAC
    GTAAACCCAG AGAATGAGGA TGGAGATGCT GCTGAAGGAG AAAAACCTCA CATGATTTTC
    AGGCCCCCTG TGCTAACTCC TATAGTGATT CCAGGCACTG AAATCATAGT GAGAGGACCC
    AGTCAAGGCT TCGGCATCAA CCCCATGTTT TCAGATGAAA CTAATCTATG CAACGGTAGG
    CCAGTAGATG GACTGACTAC TTTGCGTAAT GGGACATTAG TTGCATTTCG AGGTCATTAT
    TTCTGGATGC TGACTCCATT TACTCCACCA CCTCCACCTC GGAGAATTAC TGAAGTTTGG
    GGCATTCCCT CCCCCATTGA TACTGTTTTT ACTAGATGCA ACTGTGAAGG AAAAACTTTC
    TTCTTTAAGG GTTCTCAGTA CTGGCGTTTC ACCAATGATA TAAAAGATGC AGGATATCCC
    AAACTAATTT CCAAAGGATT TGGAGGACTG AATGGAAAAA TAGTGGCAGC CCTCTCAATA
    GCTCAGTACA AGAGCAGACC TGAATCCGTG TATTTTTTCA AGAGAGGTGG CAGCGTTCAG
    CAATACACTT ACAAACAGGA ACCCACCCAA AAGTGTACTG GAAGAAGGCC TGCGATAAAC
    TATTCTGTGT ATGGAGAAAC AGCACAGGTT AGGAGACGTC GATTTGAACG TGCTATAGGA
    CCTTCTCAAG TACACACCAT CAGGATTCAC TACACACCCG TCAGAGTCCC TTATCAAGAC
    AAAGGTTTCC TCCATAATGA AGTTAAAGTG AGTACACTGT GGAGAGGACT TCCAAATGTG
    GTTACTTCAG CAATATCACT GCCCAACATC AGAAAACCCG ATGGCTATGA TTACTATGCC
    CTTTCTAAAG ATCAATACTA TAACATCGAC GTGCCAAGTA GGACAGCAAG AGCCATTACT
    ACTCGTTCTG GGCAGACCTT ATCCAATACC TGGTACAACT GTCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831763.1
    CDS28..4455
    Translation

    Target ORF information:

    RefSeq Version XM_024975995.1
    Organism Bos taurus(cattle)
    Definition Bos taurus proteoglycan 4 (PRG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTAAA AGATAAGAAG AAAAGAACTC CTAAAAATAA ACCTACCCCA
    GAACCACCAG TTATAGATGA AGATGGAAGT GGACTGGATA ATGGTGATTT CAAGCTCACC
    CCAACTCCTA ACATTCCCAC CACACAACGT AATAAAGTGA CCACAACTCC CAAGATTACA
    ACAGTAAAAC CAACACTTCC TAAGCCCAGT CTTCCACCTA ATTCTGATAC AACTAAAGAG
    ATACCTTCGA CAGTCAACAG AGAGACAACA GTTGAAACTA AAGAGACTTC TACAACAAAT
    AAACCGACTT CCACTAGCGC AAAAGAGAAG ACAACTTCAG CTAAAGAGAC ACGAACTGCA
    GAGAATACAT CTACTAAAGG TCTTGCACCC ACACCTGAAG TTCCTGCTAT ATCTACACCC
    AAAGCTGAAA CTATAACCAG ATCCCCTACA CCCACCACCC CCAAGGAGCC TGCTCCCACC
    ACCAAGGAGC CTGCTCTCAC CACCACCACC AAGGAGCCTG CTTCCACCAC CCCAAAGAAG
    CCTGCACCCA CCACCCCTAA GGAGACTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    AAGGAGCCTG AACCCACCAC CCCCAAGCAG CCTGCTCCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCC CTAAGGAGAC TGCACCCACC ACCAAGGAGC CTGAACCCAC CACCCCCAAG
    CAGCCTGCTC CCACCACCCC CAAGCAGCCT GCACCCACCA CCTCTAAGGA GACTGCACCC
    ACCACCAAGG AGCCTGCTCC CACCACCCCC AAGGAGCCTG CTCCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCCCCAA GGAGCCTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    CCCAAGCAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCAC CCACCACCCC TAAGGAGACT
    GCACCCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCACCCA CCACCCCCAA GGAGCCTGCA
    CCCACCACCA AGGAGCCTGA ATCCACCACC AAGGAGCCTG AACCCACCAC CAAGGAGCCT
    GAACCCACCA CCCCCAAGCA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC ACCCACCACC
    CCCAAGGAGA CTGCACCCAC CACCAAGGAG CCTGCACCCA CCACCCCCAA GCAGCCTGCT
    CCCACCACCC CCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCACC CACCACCCCC
    AAGGAGCCTG CACCCACCAC CAAGGAGCCT GCACCCACCA CCAAGGAGCC TGAACCCACC
    ACCAAGGAGC CTGCACCCAC TACCACCCCC AAGGAGCCTG CACCCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCCTCA AGCAGCCTGC TCCCACCACC
    CCCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCTC CCACCACCCC CAAGGAGCCT
    ACTCCCACCA CCCCCAAGGA GCCTGCTCCC ACCACCCCCA AGGAGCCTGC TCCCACCACC
    CCCAAGGAGC CTACTCCCAC TACTCCCAAG GAGCCTGCTC CCACCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCACCCAC CATCCCCAAG GAACCTGCAC CCACCACCCC CAAAGAGCCT
    GCTCCCACCA CCCACAAGGA GCCTACACCC ACCACCACCC CCAAAGAGCC TGCTCCCACC
    AGCCCCAAGG AACCTGCTCC CACCAGCCCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCCC CAAGGAGCCT GCTCCCACCG ATCCCAAGGA GCCTGCTCCC
    GCTGAACCCA AGGAGCCTGC ACCCACCAGC TCCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCTC CGACCGATCC CAAGGAGCCT
    GCTCCCGCTG AACCCAAGGA GCCTGCACCC ACCAGCCCCA AGGAGCCTGC ACCCACTACC
    ACCACCAAGG AGCCTGCTCC CGCTGAACCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCAC CAAGGAGCCT GCTCCCGCTG AACCCAAGGA GCCTGCACCC
    ACCAGCCCCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCTCC CACTACCACC
    CCCAAGGAGC CTGCTCCGAC CGATCCCAAG GAGCCTGCTC CCGCTGAACC CAAGGAGCCA
    GCTCCTGCTG AACCCAAGGA GCCCACTCCA AATTCTCCTG AGACACTTGC TCCAAGTTCT
    CCTGATATCC CTGCTCCAAC CACCTCAGAG GCCTCTACTT CAACTACCAC CGTGGAGCCT
    TCCACTACTC TGAAGAGCCC TGCTGAATCA ACTCCTCAAC CTCCTATAGA ACCCACACCA
    AAGGCTCTTG AAAACAGTCC CAAGGAACCT GCAGTACCCA CAATCAAGGG TCCTAAAGTG
    AACAAACCTG AAATAACTAC AACAGTTAAA GACAAGTCCA CAGAAAAAGA CATATATTCT
    ACACCTGAAA TTACAACAGC TGTAGCTAAG ATTATAACAG AGGCAGCAAC TAAAACAGAA
    AAGACTACTG AATCGAAAAT ACCAAGTACA ACCACAGATG TAACATCTAC CACAACCAAA
    GATACCACGT CATCCAAAAT TACTCCTAAA GCAACTCTTG CACCTAAAGT AATGACTGCA
    ACAAAGAAGA CGACCGAAAA GACTAAGAAC AAACCTGAAA AAACCACAGC TGTACCAAAA
    GGCAGAGCTA CTAATTCTCA AGTGACAACT CCTAAACCGC AGAAACCAAC CAAAGCACCC
    AAAAAGCCCA CTTCGACCAA AAAGCCAAGA ACACCTAGAG TGAGAAAACC AAAGACTACG
    CCAACGCCCC CAAAGACGAC GACATCAGCA ATGCCTGAAC CGACTCCTAC CTCTTTACCA
    GAAGCCATGC TCCAAACCAC CACCAGGCCT ACCCCAACCC CCAACTCAGA AATAATTGAC
    GTAAACCCAG AGAATGAGGA TGGAGATGCT GCTGAAGGAG AAAAACCTCA CATGATTTTC
    AGGCCCCCTG TGCTAACTCC TATAGTGATT CCAGGCACTG AAATCATAGT GAGAGGACCC
    AGTCAAGGCT TCGGCATCAA CCCCATGTTT TCAGATGAAA CTAATCTATG CAACGGTAGG
    CCAGTAGATG GACTGACTAC TTTGCGTAAT GGGACATTAG TTGCATTTCG AGGTCATTAT
    TTCTGGATGC TGACTCCATT TACTCCACCA CCTCCACCTC GGAGAATTAC TGAAGTTTGG
    GGCATTCCCT CCCCCATTGA TACTGTTTTT ACTAGATGCA ACTGTGAAGG AAAAACTTTC
    TTCTTTAAGG GTTCTCAGTA CTGGCGTTTC ACCAATGATA TAAAAGATGC AGGATATCCC
    AAACTAATTT CCAAAGGATT TGGAGGACTG AATGGAAAAA TAGTGGCAGC CCTCTCAATA
    GCTCAGTACA AGAGCAGACC TGAATCCGTG TATTTTTTCA AGAGAGGTGG CAGCGTTCAG
    CAATACACTT ACAAACAGGA ACCCACCCAA AAGTGTACTG GAAGAAGGCC TGCGATAAAC
    TATTCTGTGT ATGGAGAAAC AGCACAGGTT AGGAGACGTC GATTTGAACG TGCTATAGGA
    CCTTCTCAAG TACACACCAT CAGGATTCAC TACACACCCG TCAGAGTCCC TTATCAAGAC
    AAAGGTTTCC TCCATAATGA AGTTAAAGTG AGTACACTGT GGAGAGGACT TCCAAATGTG
    GTTACTTCAG CAATATCACT GCCCAACATC AGAAAACCCG ATGGCTATGA TTACTATGCC
    CTTTCTAAAG ATCAATACTA TAACATCGAC GTGCCAAGTA GGACAGCAAG AGCCATTACT
    ACTCGTTCTG GGCAGACCTT ATCCAATACC TGGTACAACT GTCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223400
    Clone ID Related Accession (Same CDS sequence) XM_024975996.1
    Accession Version XM_024975996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product proteoglycan 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGAGCTTTC CTGTAAAGGC CGCTGCTTCG AAACCTTTGC AAGAGGGAGG
    GAGTGTGACT GCGACTCAGA CTGCAAGAAG TATGGCAAGT GCTGTTCCGA TTATGGCACT
    TTTTGTGAAG CAGTAAAAGA TAAGAAGAAA AGAACTCCTA AAAATAAACC TACCCCAGAA
    CCACCAGTTA TAGATGAAGA TGGAAGTGGA CTGGATAATG GTGATTTCAA GCTCACCCCA
    ACTCCTAACA TTCCCACCAC ACAACGTAAT AAAGTGACCA CAACTCCCAA GATTACAACA
    GTAAAACCAA CACTTCCTAA GCCCAGTCTT CCACCTAATT CTGATACAAC TAAAGAGATA
    CCTTCGACAG TCAACAGAGA GACAACAGTT GAAACTAAAG AGACTTCTAC AACAAATAAA
    CCGACTTCCA CTAGCGCAAA AGAGAAGACA ACTTCAGCTA AAGAGACACG AACTGCAGAG
    AATACATCTA CTAAAGGTCT TGCACCCACA CCTGAAGTTC CTGCTATATC TACACCCAAA
    GCTGAAACTA TAACCAGATC CCCTACACCC ACCACCCCCA AGGAGCCTGC TCCCACCACC
    AAGGAGCCTG CTCTCACCAC CACCACCAAG GAGCCTGCTT CCACCACCCC AAAGAAGCCT
    GCACCCACCA CCCCTAAGGA GACTGCACCC ACCACCAAGG AGCCTGAACC CACCACCAAG
    GAGCCTGAAC CCACCACCCC CAAGCAGCCT GCTCCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCCCTA AGGAGACTGC ACCCACCACC AAGGAGCCTG AACCCACCAC CCCCAAGCAG
    CCTGCTCCCA CCACCCCCAA GCAGCCTGCA CCCACCACCT CTAAGGAGAC TGCACCCACC
    ACCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCTC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCAAGG AGCCTGAACC CACCACCCCC
    AAGCAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCACCCA CCACCCCTAA GGAGACTGCA
    CCCACCCCCA AGGAGCCTGC TCCCACCACC CCCAAGGAGC CTGCTCCCAC CACCCCCAAG
    GAGCCTGCTC CCACCACCCC CAAGGAGCCT GCACCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCAAGG AGCCTGAATC CACCACCAAG GAGCCTGAAC CCACCACCAA GGAGCCTGAA
    CCCACCACCC CCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCACC CACCACCCCC
    AAGGAGACTG CACCCACCAC CAAGGAGCCT GCACCCACCA CCCCCAAGCA GCCTGCTCCC
    ACCACCCCCA AGCAGCCTGC TCCCACCACC CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    GAGCCTGCAC CCACCACCAA GGAGCCTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCCTCAAGC AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCTCCCA CCACCCCCAA GGAGCCTACT
    CCCACCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTA CTCCCACTAC TCCCAAGGAG CCTGCTCCCA CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACCAT CCCCAAGGAA CCTGCACCCA CCACCCCCAA AGAGCCTGCT
    CCCACCACCC ACAAGGAGCC TACACCCACC ACCACCCCCA AAGAGCCTGC TCCCACCAGC
    CCCAAGGAAC CTGCTCCCAC CAGCCCCAAG GAGCCTGCAC CCACCAGCCC CAAGGAGCCT
    GCACCCACTA CCACCCCCAA GGAGCCTGCT CCCACCGATC CCAAGGAGCC TGCTCCCGCT
    GAACCCAAGG AGCCTGCACC CACCAGCTCC AAGGAGCCTG CACCCACTAC CACCACCAAG
    GAGCCTGCAC CCACTACCAC CCCCAAGGAG CCTGCTCCGA CCGATCCCAA GGAGCCTGCT
    CCCGCTGAAC CCAAGGAGCC TGCACCCACC AGCCCCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCTCCCGC TGAACCCAAG GAGCCTGCAC CCACCAGCCC CAAGGAGCCT
    GCACCCACTA CCACCACCAA GGAGCCTGCT CCCGCTGAAC CCAAGGAGCC TGCACCCACC
    AGCCCCAAGG AGCCTGCACC CACTACCACC ACCAAGGAGC CTGCTCCCAC TACCACCCCC
    AAGGAGCCTG CTCCGACCGA TCCCAAGGAG CCTGCTCCCG CTGAACCCAA GGAGCCAGCT
    CCTGCTGAAC CCAAGGAGCC CACTCCAAAT TCTCCTGAGA CACTTGCTCC AAGTTCTCCT
    GATATCCCTG CTCCAACCAC CTCAGAGGCC TCTACTTCAA CTACCACCGT GGAGCCTTCC
    ACTACTCTGA AGAGCCCTGC TGAATCAACT CCTCAACCTC CTATAGAACC CACACCAAAG
    GCTCTTGAAA ACAGTCCCAA GGAACCTGCA GTACCCACAA TCAAGGGTCC TAAAGTGAAC
    AAACCTGAAA TAACTACAAC AGTTAAAGAC AAGTCCACAG AAAAAGACAT ATATTCTACA
    CCTGAAATTA CAACAGCTGT AGCTAAGATT ATAACAGAGG CAGCAACTAA AACAGAAAAG
    ACTACTGAAT CGAAAATACC AAGTACAACC ACAGATGTAA CATCTACCAC AACCAAAGAT
    ACCACGTCAT CCAAAATTAC TCCTAAAGCA ACTCTTGCAC CTAAAGTAAT GACTGCAACA
    AAGAAGACGA CCGAAAAGAC TAAGAACAAA CCTGAAAAAA CCACAGCTGT ACCAAAAGGC
    AGAGCTACTA ATTCTCAAGT GACAACTCCT AAACCGCAGA AACCAACCAA AGCACCCAAA
    AAGCCCACTT CGACCAAAAA GCCAAGAACA CCTAGAGTGA GAAAACCAAA GACTACGCCA
    ACGCCCCCAA AGACGACGAC ATCAGCAATG CCTGAACCGA CTCCTACCTC TTTACCAGAA
    GCCATGCTCC AAACCACCAC CAGGCCTACC CCAACCCCCA ACTCAGAAAT AATTGACGTA
    AACCCAGAGA ATGAGGATGG AGATGCTGCT GAAGGAGAAA AACCTCACAT GATTTTCAGG
    CCCCCTGTGC TAACTCCTAT AGTGATTCCA GGCACTGAAA TCATAGTGAG AGGACCCAGT
    CAAGGCTTCG GCATCAACCC CATGTTTTCA GATGAAACTA ATCTATGCAA CGGTAGGCCA
    GTAGATGGAC TGACTACTTT GCGTAATGGG ACATTAGTTG CATTTCGAGG TCATTATTTC
    TGGATGCTGA CTCCATTTAC TCCACCACCT CCACCTCGGA GAATTACTGA AGTTTGGGGC
    ATTCCCTCCC CCATTGATAC TGTTTTTACT AGATGCAACT GTGAAGGAAA AACTTTCTTC
    TTTAAGGGTT CTCAGTACTG GCGTTTCACC AATGATATAA AAGATGCAGG ATATCCCAAA
    CTAATTTCCA AAGGATTTGG AGGACTGAAT GGAAAAATAG TGGCAGCCCT CTCAATAGCT
    CAGTACAAGA GCAGACCTGA ATCCGTGTAT TTTTTCAAGA GAGGTGGCAG CGTTCAGCAA
    TACACTTACA AACAGGAACC CACCCAAAAG TGTACTGGAA GAAGGCCTGC GATAAACTAT
    TCTGTGTATG GAGAAACAGC ACAGGTTAGG AGACGTCGAT TTGAACGTGC TATAGGACCT
    TCTCAAGTAC ACACCATCAG GATTCACTAC ACACCCGTCA GAGTCCCTTA TCAAGACAAA
    GGTTTCCTCC ATAATGAAGT TAAAGTGAGT ACACTGTGGA GAGGACTTCC AAATGTGGTT
    ACTTCAGCAA TATCACTGCC CAACATCAGA AAACCCGATG GCTATGATTA CTATGCCCTT
    TCTAAAGATC AATACTATAA CATCGACGTG CCAAGTAGGA CAGCAAGAGC CATTACTACT
    CGTTCTGGGC AGACCTTATC CAATACCTGG TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831764.1
    CDS534..4838
    Translation

    Target ORF information:

    RefSeq Version XM_024975996.1
    Organism Bos taurus(cattle)
    Definition Bos taurus proteoglycan 4 (PRG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGAGCTTTC CTGTAAAGGC CGCTGCTTCG AAACCTTTGC AAGAGGGAGG
    GAGTGTGACT GCGACTCAGA CTGCAAGAAG TATGGCAAGT GCTGTTCCGA TTATGGCACT
    TTTTGTGAAG CAGTAAAAGA TAAGAAGAAA AGAACTCCTA AAAATAAACC TACCCCAGAA
    CCACCAGTTA TAGATGAAGA TGGAAGTGGA CTGGATAATG GTGATTTCAA GCTCACCCCA
    ACTCCTAACA TTCCCACCAC ACAACGTAAT AAAGTGACCA CAACTCCCAA GATTACAACA
    GTAAAACCAA CACTTCCTAA GCCCAGTCTT CCACCTAATT CTGATACAAC TAAAGAGATA
    CCTTCGACAG TCAACAGAGA GACAACAGTT GAAACTAAAG AGACTTCTAC AACAAATAAA
    CCGACTTCCA CTAGCGCAAA AGAGAAGACA ACTTCAGCTA AAGAGACACG AACTGCAGAG
    AATACATCTA CTAAAGGTCT TGCACCCACA CCTGAAGTTC CTGCTATATC TACACCCAAA
    GCTGAAACTA TAACCAGATC CCCTACACCC ACCACCCCCA AGGAGCCTGC TCCCACCACC
    AAGGAGCCTG CTCTCACCAC CACCACCAAG GAGCCTGCTT CCACCACCCC AAAGAAGCCT
    GCACCCACCA CCCCTAAGGA GACTGCACCC ACCACCAAGG AGCCTGAACC CACCACCAAG
    GAGCCTGAAC CCACCACCCC CAAGCAGCCT GCTCCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCCCTA AGGAGACTGC ACCCACCACC AAGGAGCCTG AACCCACCAC CCCCAAGCAG
    CCTGCTCCCA CCACCCCCAA GCAGCCTGCA CCCACCACCT CTAAGGAGAC TGCACCCACC
    ACCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCTC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCCCCAAGGA GCCTGCACCC ACCACCAAGG AGCCTGAACC CACCACCCCC
    AAGCAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCACCCA CCACCCCTAA GGAGACTGCA
    CCCACCCCCA AGGAGCCTGC TCCCACCACC CCCAAGGAGC CTGCTCCCAC CACCCCCAAG
    GAGCCTGCTC CCACCACCCC CAAGGAGCCT GCACCCACCA CCCCCAAGGA GCCTGCACCC
    ACCACCAAGG AGCCTGAATC CACCACCAAG GAGCCTGAAC CCACCACCAA GGAGCCTGAA
    CCCACCACCC CCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCACC CACCACCCCC
    AAGGAGACTG CACCCACCAC CAAGGAGCCT GCACCCACCA CCCCCAAGCA GCCTGCTCCC
    ACCACCCCCA AGCAGCCTGC TCCCACCACC CCCAAGGAGC CTGCACCCAC CACCCCCAAG
    GAGCCTGCAC CCACCACCAA GGAGCCTGCA CCCACCACCA AGGAGCCTGA ACCCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCCTCAAGC AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTGCTCCCA CCACCCCCAA GGAGCCTACT
    CCCACCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTA CTCCCACTAC TCCCAAGGAG CCTGCTCCCA CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACCAT CCCCAAGGAA CCTGCACCCA CCACCCCCAA AGAGCCTGCT
    CCCACCACCC ACAAGGAGCC TACACCCACC ACCACCCCCA AAGAGCCTGC TCCCACCAGC
    CCCAAGGAAC CTGCTCCCAC CAGCCCCAAG GAGCCTGCAC CCACCAGCCC CAAGGAGCCT
    GCACCCACTA CCACCCCCAA GGAGCCTGCT CCCACCGATC CCAAGGAGCC TGCTCCCGCT
    GAACCCAAGG AGCCTGCACC CACCAGCTCC AAGGAGCCTG CACCCACTAC CACCACCAAG
    GAGCCTGCAC CCACTACCAC CCCCAAGGAG CCTGCTCCGA CCGATCCCAA GGAGCCTGCT
    CCCGCTGAAC CCAAGGAGCC TGCACCCACC AGCCCCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCTCCCGC TGAACCCAAG GAGCCTGCAC CCACCAGCCC CAAGGAGCCT
    GCACCCACTA CCACCACCAA GGAGCCTGCT CCCGCTGAAC CCAAGGAGCC TGCACCCACC
    AGCCCCAAGG AGCCTGCACC CACTACCACC ACCAAGGAGC CTGCTCCCAC TACCACCCCC
    AAGGAGCCTG CTCCGACCGA TCCCAAGGAG CCTGCTCCCG CTGAACCCAA GGAGCCAGCT
    CCTGCTGAAC CCAAGGAGCC CACTCCAAAT TCTCCTGAGA CACTTGCTCC AAGTTCTCCT
    GATATCCCTG CTCCAACCAC CTCAGAGGCC TCTACTTCAA CTACCACCGT GGAGCCTTCC
    ACTACTCTGA AGAGCCCTGC TGAATCAACT CCTCAACCTC CTATAGAACC CACACCAAAG
    GCTCTTGAAA ACAGTCCCAA GGAACCTGCA GTACCCACAA TCAAGGGTCC TAAAGTGAAC
    AAACCTGAAA TAACTACAAC AGTTAAAGAC AAGTCCACAG AAAAAGACAT ATATTCTACA
    CCTGAAATTA CAACAGCTGT AGCTAAGATT ATAACAGAGG CAGCAACTAA AACAGAAAAG
    ACTACTGAAT CGAAAATACC AAGTACAACC ACAGATGTAA CATCTACCAC AACCAAAGAT
    ACCACGTCAT CCAAAATTAC TCCTAAAGCA ACTCTTGCAC CTAAAGTAAT GACTGCAACA
    AAGAAGACGA CCGAAAAGAC TAAGAACAAA CCTGAAAAAA CCACAGCTGT ACCAAAAGGC
    AGAGCTACTA ATTCTCAAGT GACAACTCCT AAACCGCAGA AACCAACCAA AGCACCCAAA
    AAGCCCACTT CGACCAAAAA GCCAAGAACA CCTAGAGTGA GAAAACCAAA GACTACGCCA
    ACGCCCCCAA AGACGACGAC ATCAGCAATG CCTGAACCGA CTCCTACCTC TTTACCAGAA
    GCCATGCTCC AAACCACCAC CAGGCCTACC CCAACCCCCA ACTCAGAAAT AATTGACGTA
    AACCCAGAGA ATGAGGATGG AGATGCTGCT GAAGGAGAAA AACCTCACAT GATTTTCAGG
    CCCCCTGTGC TAACTCCTAT AGTGATTCCA GGCACTGAAA TCATAGTGAG AGGACCCAGT
    CAAGGCTTCG GCATCAACCC CATGTTTTCA GATGAAACTA ATCTATGCAA CGGTAGGCCA
    GTAGATGGAC TGACTACTTT GCGTAATGGG ACATTAGTTG CATTTCGAGG TCATTATTTC
    TGGATGCTGA CTCCATTTAC TCCACCACCT CCACCTCGGA GAATTACTGA AGTTTGGGGC
    ATTCCCTCCC CCATTGATAC TGTTTTTACT AGATGCAACT GTGAAGGAAA AACTTTCTTC
    TTTAAGGGTT CTCAGTACTG GCGTTTCACC AATGATATAA AAGATGCAGG ATATCCCAAA
    CTAATTTCCA AAGGATTTGG AGGACTGAAT GGAAAAATAG TGGCAGCCCT CTCAATAGCT
    CAGTACAAGA GCAGACCTGA ATCCGTGTAT TTTTTCAAGA GAGGTGGCAG CGTTCAGCAA
    TACACTTACA AACAGGAACC CACCCAAAAG TGTACTGGAA GAAGGCCTGC GATAAACTAT
    TCTGTGTATG GAGAAACAGC ACAGGTTAGG AGACGTCGAT TTGAACGTGC TATAGGACCT
    TCTCAAGTAC ACACCATCAG GATTCACTAC ACACCCGTCA GAGTCCCTTA TCAAGACAAA
    GGTTTCCTCC ATAATGAAGT TAAAGTGAGT ACACTGTGGA GAGGACTTCC AAATGTGGTT
    ACTTCAGCAA TATCACTGCC CAACATCAGA AAACCCGATG GCTATGATTA CTATGCCCTT
    TCTAAAGATC AATACTATAA CATCGACGTG CCAAGTAGGA CAGCAAGAGC CATTACTACT
    CGTTCTGGGC AGACCTTATC CAATACCTGG TACAACTGTC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223401
    Clone ID Related Accession (Same CDS sequence) XM_024975998.1
    Accession Version XM_024975998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product proteoglycan 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTGCA TAACCCCACA ACACCACCTC CTCCAAAGAC TGCACCTCCA
    CCTCCAGGAG CATCTCAGAC CATCAAGTCA ACAACCAAAC GTTCACCCAA ATCACCAAAC
    AAGAAGAAGA TTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCTTCCTC CTCCTCTTCC TCTTCCTCTT CAACTATTCG GAAAATCAAG
    TCTTCCAAAA ATTCAGCTGC TAATAGAGAA TTAAAGAAGA AACCCAAAGT AAAAGATAAG
    AAGAAAAGAA CTCCTAAAAA TAAACCTACC CCAGAACCAC CAGTTATAGA TGAAGATGGA
    AGTGGACTGG ATAATGGTGA TTTCAAGCTC ACCCCAACTC CTAACATTCC CACCACACAA
    CGTAATAAAG TGACCACAAC TCCCAAGATT ACAACAGTAA AACCAACACT TCCTAAGCCC
    AGTCTTCCAC CTAATTCTGA TACAACTAAA GAGATACCTT CGACAGTCAA CAGAGAGACA
    ACAGTTGAAA CTAAAGAGAC TTCTACAACA AATAAACCGA CTTCCACTAG CGCAAAAGAG
    AAGACAACTT CAGCTAAAGA GACACGAACT GCAGAGAATA CATCTACTAA AGGTCTTGCA
    CCCACACCTG AAGTTCCTGC TATATCTACA CCCAAAGCTG AAACTATAAC CAGATCCCCT
    ACACCCACCA CCCCCAAGGA GCCTGCTCCC ACCACCAAGG AGCCTGCTCT CACCACCACC
    ACCAAGGAGC CTGCTTCCAC CACCCCAAAG AAGCCTGCAC CCACCACCCC TAAGGAGACT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCAAGGAGC CTGAACCCAC CACCCCCAAG
    CAGCCTGCTC CCACCACCCC CAAGGAGCCT GCACCCACCA CCCCTAAGGA GACTGCACCC
    ACCACCAAGG AGCCTGAACC CACCACCCCC AAGCAGCCTG CTCCCACCAC CCCCAAGCAG
    CCTGCACCCA CCACCTCTAA GGAGACTGCA CCCACCACCA AGGAGCCTGC TCCCACCACC
    CCCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCCCCAAGC AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CACCCACCAC CCCTAAGGAG ACTGCACCCA CCCCCAAGGA GCCTGCTCCC
    ACCACCCCCA AGGAGCCTGC TCCCACCACC CCCAAGGAGC CTGCTCCCAC CACCCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGGAGCCT GCACCCACCA CCAAGGAGCC TGAATCCACC
    ACCAAGGAGC CTGAACCCAC CACCAAGGAG CCTGAACCCA CCACCCCCAA GCAGCCTGCT
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCCCCAAGG AGACTGCACC CACCACCAAG
    GAGCCTGCAC CCACCACCCC CAAGCAGCCT GCTCCCACCA CCCCCAAGCA GCCTGCTCCC
    ACCACCCCCA AGGAGCCTGC ACCCACCACC CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    CCCAAGGAGC CTGCACCCAC CACCCCCAAG GAGCCTGCAC CCACCACCAA GGAGCCTGAA
    CCCACCACCC TCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTACTCCCA CCACCCCCAA GGAGCCTGCT
    CCCACCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTACTCC CACTACTCCC
    AAGGAGCCTG CTCCCACCAC CACCCCCAAG GAGCCTGCAC CCACCACCAA GGAGCCTGAA
    CCCACCACCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCACC CACCATCCCC
    AAGGAACCTG CACCCACCAC CCCCAAAGAG CCTGCTCCCA CCACCCACAA GGAGCCTACA
    CCCACCACCA CCCCCAAAGA GCCTGCTCCC ACCAGCCCCA AGGAACCTGC TCCCACCAGC
    CCCAAGGAGC CTGCACCCAC CAGCCCCAAG GAGCCTGCAC CCACTACCAC CCCCAAGGAG
    CCTGCTCCCA CCGATCCCAA GGAGCCTGCT CCCGCTGAAC CCAAGGAGCC TGCACCCACC
    AGCTCCAAGG AGCCTGCACC CACTACCACC ACCAAGGAGC CTGCACCCAC TACCACCCCC
    AAGGAGCCTG CTCCGACCGA TCCCAAGGAG CCTGCTCCCG CTGAACCCAA GGAGCCTGCA
    CCCACCAGCC CCAAGGAGCC TGCACCCACT ACCACCACCA AGGAGCCTGC TCCCGCTGAA
    CCCAAGGAGC CTGCACCCAC CAGCCCCAAG GAGCCTGCAC CCACTACCAC CACCAAGGAG
    CCTGCTCCCG CTGAACCCAA GGAGCCTGCA CCCACCAGCC CCAAGGAGCC TGCACCCACT
    ACCACCACCA AGGAGCCTGC TCCCACTACC ACCCCCAAGG AGCCTGCTCC GACCGATCCC
    AAGGAGCCTG CTCCCGCTGA ACCCAAGGAG CCAGCTCCTG CTGAACCCAA GGAGCCCACT
    CCAAATTCTC CTGAGACACT TGCTCCAAGT TCTCCTGATA TCCCTGCTCC AACCACCTCA
    GAGGCCTCTA CTTCAACTAC CACCGTGGAG CCTTCCACTA CTCTGAAGAG CCCTGCTGAA
    TCAACTCCTC AACCTCCTAT AGAACCCACA CCAAAGGCTC TTGAAAACAG TCCCAAGGAA
    CCTGCAGTAC CCACAATCAA GGGTCCTAAA GTGAACAAAC CTGAAATAAC TACAACAGTT
    AAAGACAAGT CCACAGAAAA AGACATATAT TCTACACCTG AAATTACAAC AGCTGTAGCT
    AAGATTATAA CAGAGGCAGC AACTAAAACA GAAAAGACTA CTGAATCGAA AATACCAAGT
    ACAACCACAG ATGTAACATC TACCACAACC AAAGATACCA CGTCATCCAA AATTACTCCT
    AAAGCAACTC TTGCACCTAA AGTAATGACT GCAACAAAGA AGACGACCGA AAAGACTAAG
    AACAAACCTG AAAAAACCAC AGCTGTACCA AAAGGCAGAG CTACTAATTC TCAAGTGACA
    ACTCCTAAAC CGCAGAAACC AACCAAAGCA CCCAAAAAGC CCACTTCGAC CAAAAAGCCA
    AGAACACCTA GAGTGAGAAA ACCAAAGACT ACGCCAACGC CCCCAAAGAC GACGACATCA
    GCAATGCCTG AACCGACTCC TACCTCTTTA CCAGAAGCCA TGCTCCAAAC CACCACCAGG
    CCTACCCCAA CCCCCAACTC AGAAATAATT GACGTAAACC CAGAGAATGA GGATGGAGAT
    GCTGCTGAAG GAGAAAAACC TCACATGATT TTCAGGCCCC CTGTGCTAAC TCCTATAGTG
    ATTCCAGGCA CTGAAATCAT AGTGAGAGGA CCCAGTCAAG GCTTCGGCAT CAACCCCATG
    TTTTCAGATG AAACTAATCT ATGCAACGGT AGGCCAGTAG ATGGACTGAC TACTTTGCGT
    AATGGGACAT TAGTTGCATT TCGAGGTCAT TATTTCTGGA TGCTGACTCC ATTTACTCCA
    CCACCTCCAC CTCGGAGAAT TACTGAAGTT TGGGGCATTC CCTCCCCCAT TGATACTGTT
    TTTACTAGAT GCAACTGTGA AGGAAAAACT TTCTTCTTTA AGGGTTCTCA GTACTGGCGT
    TTCACCAATG ATATAAAAGA TGCAGGATAT CCCAAACTAA TTTCCAAAGG ATTTGGAGGA
    CTGAATGGAA AAATAGTGGC AGCCCTCTCA ATAGCTCAGT ACAAGAGCAG ACCTGAATCC
    GTGTATTTTT TCAAGAGAGG TGGCAGCGTT CAGCAATACA CTTACAAACA GGAACCCACC
    CAAAAGTGTA CTGGAAGAAG GCCTGCGATA AACTATTCTG TGTATGGAGA AACAGCACAG
    GTTAGGAGAC GTCGATTTGA ACGTGCTATA GGACCTTCTC AAGTACACAC CATCAGGATT
    CACTACACAC CCGTCAGAGT CCCTTATCAA GACAAAGGTT TCCTCCATAA TGAAGTTAAA
    GTGAGTACAC TGTGGAGAGG ACTTCCAAAT GTGGTTACTT CAGCAATATC ACTGCCCAAC
    ATCAGAAAAC CCGATGGCTA TGATTACTAT GCCCTTTCTA AAGATCAATA CTATAACATC
    GACGTGCCAA GTAGGACAGC AAGAGCCATT ACTACTCGTT CTGGGCAGAC CTTATCCAAT
    ACCTGGTACA ACTGTCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831766.1
    CDS543..5243
    Translation

    Target ORF information:

    RefSeq Version XM_024975998.1
    Organism Bos taurus(cattle)
    Definition Bos taurus proteoglycan 4 (PRG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTGCA TAACCCCACA ACACCACCTC CTCCAAAGAC TGCACCTCCA
    CCTCCAGGAG CATCTCAGAC CATCAAGTCA ACAACCAAAC GTTCACCCAA ATCACCAAAC
    AAGAAGAAGA TTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCTTCCTC CTCCTCTTCC TCTTCCTCTT CAACTATTCG GAAAATCAAG
    TCTTCCAAAA ATTCAGCTGC TAATAGAGAA TTAAAGAAGA AACCCAAAGT AAAAGATAAG
    AAGAAAAGAA CTCCTAAAAA TAAACCTACC CCAGAACCAC CAGTTATAGA TGAAGATGGA
    AGTGGACTGG ATAATGGTGA TTTCAAGCTC ACCCCAACTC CTAACATTCC CACCACACAA
    CGTAATAAAG TGACCACAAC TCCCAAGATT ACAACAGTAA AACCAACACT TCCTAAGCCC
    AGTCTTCCAC CTAATTCTGA TACAACTAAA GAGATACCTT CGACAGTCAA CAGAGAGACA
    ACAGTTGAAA CTAAAGAGAC TTCTACAACA AATAAACCGA CTTCCACTAG CGCAAAAGAG
    AAGACAACTT CAGCTAAAGA GACACGAACT GCAGAGAATA CATCTACTAA AGGTCTTGCA
    CCCACACCTG AAGTTCCTGC TATATCTACA CCCAAAGCTG AAACTATAAC CAGATCCCCT
    ACACCCACCA CCCCCAAGGA GCCTGCTCCC ACCACCAAGG AGCCTGCTCT CACCACCACC
    ACCAAGGAGC CTGCTTCCAC CACCCCAAAG AAGCCTGCAC CCACCACCCC TAAGGAGACT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCAAGGAGC CTGAACCCAC CACCCCCAAG
    CAGCCTGCTC CCACCACCCC CAAGGAGCCT GCACCCACCA CCCCTAAGGA GACTGCACCC
    ACCACCAAGG AGCCTGAACC CACCACCCCC AAGCAGCCTG CTCCCACCAC CCCCAAGCAG
    CCTGCACCCA CCACCTCTAA GGAGACTGCA CCCACCACCA AGGAGCCTGC TCCCACCACC
    CCCAAGGAGC CTGCTCCCAC CACCCCCAAG GAGCCTGCAC CCACCACCCC CAAGGAGCCT
    GCACCCACCA CCAAGGAGCC TGAACCCACC ACCCCCAAGC AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CACCCACCAC CCCTAAGGAG ACTGCACCCA CCCCCAAGGA GCCTGCTCCC
    ACCACCCCCA AGGAGCCTGC TCCCACCACC CCCAAGGAGC CTGCTCCCAC CACCCCCAAG
    GAGCCTGCAC CCACCACCCC CAAGGAGCCT GCACCCACCA CCAAGGAGCC TGAATCCACC
    ACCAAGGAGC CTGAACCCAC CACCAAGGAG CCTGAACCCA CCACCCCCAA GCAGCCTGCT
    CCCACCACCC CCAAGGAGCC TGCACCCACC ACCCCCAAGG AGACTGCACC CACCACCAAG
    GAGCCTGCAC CCACCACCCC CAAGCAGCCT GCTCCCACCA CCCCCAAGCA GCCTGCTCCC
    ACCACCCCCA AGGAGCCTGC ACCCACCACC CCCAAGGAGC CTGCACCCAC CACCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    CCCAAGGAGC CTGCACCCAC CACCCCCAAG GAGCCTGCAC CCACCACCAA GGAGCCTGAA
    CCCACCACCC TCAAGCAGCC TGCTCCCACC ACCCCCAAGG AGCCTGCTCC CACCACCCCC
    AAGGAGCCTG CTCCCACCAC CCCCAAGGAG CCTACTCCCA CCACCCCCAA GGAGCCTGCT
    CCCACCACCC CCAAGGAGCC TGCTCCCACC ACCCCCAAGG AGCCTACTCC CACTACTCCC
    AAGGAGCCTG CTCCCACCAC CACCCCCAAG GAGCCTGCAC CCACCACCAA GGAGCCTGAA
    CCCACCACCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCACC CACCATCCCC
    AAGGAACCTG CACCCACCAC CCCCAAAGAG CCTGCTCCCA CCACCCACAA GGAGCCTACA
    CCCACCACCA CCCCCAAAGA GCCTGCTCCC ACCAGCCCCA AGGAACCTGC TCCCACCAGC
    CCCAAGGAGC CTGCACCCAC CAGCCCCAAG GAGCCTGCAC CCACTACCAC CCCCAAGGAG
    CCTGCTCCCA CCGATCCCAA GGAGCCTGCT CCCGCTGAAC CCAAGGAGCC TGCACCCACC
    AGCTCCAAGG AGCCTGCACC CACTACCACC ACCAAGGAGC CTGCACCCAC TACCACCCCC
    AAGGAGCCTG CTCCGACCGA TCCCAAGGAG CCTGCTCCCG CTGAACCCAA GGAGCCTGCA
    CCCACCAGCC CCAAGGAGCC TGCACCCACT ACCACCACCA AGGAGCCTGC TCCCGCTGAA
    CCCAAGGAGC CTGCACCCAC CAGCCCCAAG GAGCCTGCAC CCACTACCAC CACCAAGGAG
    CCTGCTCCCG CTGAACCCAA GGAGCCTGCA CCCACCAGCC CCAAGGAGCC TGCACCCACT
    ACCACCACCA AGGAGCCTGC TCCCACTACC ACCCCCAAGG AGCCTGCTCC GACCGATCCC
    AAGGAGCCTG CTCCCGCTGA ACCCAAGGAG CCAGCTCCTG CTGAACCCAA GGAGCCCACT
    CCAAATTCTC CTGAGACACT TGCTCCAAGT TCTCCTGATA TCCCTGCTCC AACCACCTCA
    GAGGCCTCTA CTTCAACTAC CACCGTGGAG CCTTCCACTA CTCTGAAGAG CCCTGCTGAA
    TCAACTCCTC AACCTCCTAT AGAACCCACA CCAAAGGCTC TTGAAAACAG TCCCAAGGAA
    CCTGCAGTAC CCACAATCAA GGGTCCTAAA GTGAACAAAC CTGAAATAAC TACAACAGTT
    AAAGACAAGT CCACAGAAAA AGACATATAT TCTACACCTG AAATTACAAC AGCTGTAGCT
    AAGATTATAA CAGAGGCAGC AACTAAAACA GAAAAGACTA CTGAATCGAA AATACCAAGT
    ACAACCACAG ATGTAACATC TACCACAACC AAAGATACCA CGTCATCCAA AATTACTCCT
    AAAGCAACTC TTGCACCTAA AGTAATGACT GCAACAAAGA AGACGACCGA AAAGACTAAG
    AACAAACCTG AAAAAACCAC AGCTGTACCA AAAGGCAGAG CTACTAATTC TCAAGTGACA
    ACTCCTAAAC CGCAGAAACC AACCAAAGCA CCCAAAAAGC CCACTTCGAC CAAAAAGCCA
    AGAACACCTA GAGTGAGAAA ACCAAAGACT ACGCCAACGC CCCCAAAGAC GACGACATCA
    GCAATGCCTG AACCGACTCC TACCTCTTTA CCAGAAGCCA TGCTCCAAAC CACCACCAGG
    CCTACCCCAA CCCCCAACTC AGAAATAATT GACGTAAACC CAGAGAATGA GGATGGAGAT
    GCTGCTGAAG GAGAAAAACC TCACATGATT TTCAGGCCCC CTGTGCTAAC TCCTATAGTG
    ATTCCAGGCA CTGAAATCAT AGTGAGAGGA CCCAGTCAAG GCTTCGGCAT CAACCCCATG
    TTTTCAGATG AAACTAATCT ATGCAACGGT AGGCCAGTAG ATGGACTGAC TACTTTGCGT
    AATGGGACAT TAGTTGCATT TCGAGGTCAT TATTTCTGGA TGCTGACTCC ATTTACTCCA
    CCACCTCCAC CTCGGAGAAT TACTGAAGTT TGGGGCATTC CCTCCCCCAT TGATACTGTT
    TTTACTAGAT GCAACTGTGA AGGAAAAACT TTCTTCTTTA AGGGTTCTCA GTACTGGCGT
    TTCACCAATG ATATAAAAGA TGCAGGATAT CCCAAACTAA TTTCCAAAGG ATTTGGAGGA
    CTGAATGGAA AAATAGTGGC AGCCCTCTCA ATAGCTCAGT ACAAGAGCAG ACCTGAATCC
    GTGTATTTTT TCAAGAGAGG TGGCAGCGTT CAGCAATACA CTTACAAACA GGAACCCACC
    CAAAAGTGTA CTGGAAGAAG GCCTGCGATA AACTATTCTG TGTATGGAGA AACAGCACAG
    GTTAGGAGAC GTCGATTTGA ACGTGCTATA GGACCTTCTC AAGTACACAC CATCAGGATT
    CACTACACAC CCGTCAGAGT CCCTTATCAA GACAAAGGTT TCCTCCATAA TGAAGTTAAA
    GTGAGTACAC TGTGGAGAGG ACTTCCAAAT GTGGTTACTT CAGCAATATC ACTGCCCAAC
    ATCAGAAAAC CCGATGGCTA TGATTACTAT GCCCTTTCTA AAGATCAATA CTATAACATC
    GACGTGCCAA GTAGGACAGC AAGAGCCATT ACTACTCGTT CTGGGCAGAC CTTATCCAAT
    ACCTGGTACA ACTGTCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41101
    Clone ID Related Accession (Same CDS sequence) NM_001206633.1
    Accession Version NM_001206633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(cattle)
    Product proteoglycan 4 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03110221.1, AAFC03113191.1 and AAFC03110223.1. On or before May 4, 2011 this sequence version replaced XM_606494.5, XM_002694252.1. Sequence Note: This RefSeq record was created from genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on homologous protein alignment with human NP_005798.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01093729, SAMN01093731 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTGCA TAACCCCACA ACACCACCTC CTCCAAAGAC TGCACCTCCA
    CCTCCAGGAG CATCTCAGAC CATCAAGTCA ACAACCAAAC GTTCACCCAA ATCACCAAAC
    AAGAAGAAGA TTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCTTCCTC CTCCTCTTCC TCTTCCTCTT CAACTATTCG GAAAATCAAG
    TCTTCCAAAA ATTCAGCTGC TAATAGAGAA TTAAAGAAGA AACCCAAAGT AAAAGATAAG
    AAGAAAAGAA CTCCTAAAAA TAAACCTACC CCAGAACCAC CAGTTATAGA TGAAGATGGA
    AGTGGACTGG ATAATGGTGA TTTCAAGCTC ACCCCAACTC CTAACATTCC CACCACACAA
    CGTAATAAAG TGACCACAAC TCCCAAGATT ACAACAGTAA AACCAACACT TCCTAAGCCC
    AGTCTTCCAC CTAATTCTGA TACAACTAAA GAGATACCTT CGACAGTCAA CAGAGAGACA
    ACAGTTGAAA CTAAAGAGAC TTCTACAACA AATAAACCGA CTTCCACTAG CGCAAAAGAG
    AAGACAACTT CAGCTAAAGA GACACGAACT GCAGAGAATA CATCTACTAA AGGTCTTGCA
    CCCACACCTG AAGTTCCTGC TATATCTACA CCCAAAGCTG AAACTATAAC CAGATCCCCT
    ACACCCACCA CCCCCAAGGA TCCTCCTCCC ACACACCCCA AGGAGCCTGC TCCCACCAAC
    AACAAGGAGC CTACTCCCAC TACTCCCAAG GAGCCTGCTC CCACCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCACCCAC CATCCCCAAG GAACCTGCAC CCACCACCCC CAAAGAGCCT
    GCTCCCACCA CCCACAAGGA GCCTACACCC ACCACCACCC CCAAAGAGCC TGCTCCCACC
    AGCCCCAAGG AACCTGCTCC CACCAGCCCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCCC CAAGGAGCCT GCTCCCACCG ATCCCAAGGA GCCTGCTCCC
    GCTGAACCCA AGGAGCCTGC ACCCACCAGC TCCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCTC CGACCGATCC CAAGGAGCCT
    GCTCCCGCTG AACCCAAGGA GCCTGCACCC ACCAGCCCCA AGGAGCCTGC ACCCACTACC
    ACCACCAAGG AGCCTGCTCC CGCTGAACCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCAC CAAGGAGCCT GCTCCCGCTG AACCCAAGGA GCCTGCACCC
    ACCAGCCCCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCTCC CACTACCACC
    CCCAAGGAGC CTGCTCCGAC CGATCCCAAG GAGCCTGCTC CCGCTGAACC CAAGGAGCCA
    GCTCCTGCTG AACCCAAGGA GCCCACTCCA AATTCTCCTG AGACACTTGC TCCAAGTTCT
    CCTGATATCC CTGCTCCAAC CACCTCAGAG GCCTCTACTT CAACTACCAC CGTGGAGCCT
    TCCACTACTC TGAAGAGCCC TGCTGAATCA ACTCCTCAAC CTCCTATAGA ACCCACACCA
    AAGGCTCTTG AAAACAGTCC CAAGGAACCT GCAGTACCCA CAATCAAGGG TCCTAAAGTG
    AACAAACCTG AAATAACTAC AACAGTTAAA GACAAGTCCA CAGAAAAAGA CATATATTCT
    ACACCTGAAA TTACAACAGC TGTAGCTAAG ATTATAACAG AGGCAGCAAC TAAAACAGAA
    AAGACTACTG AATCGAAAAT ACCAAGTACA ACCACAGATG TAACATCTAC CACAACCAAA
    GATACCACGT CATCCAAAAT TACTCCTAAA GCAACTCTTG CACCTAAAGT AATGACTGCA
    ACAAAGAAGA CGACCGAAAA GACTAAGAAC AAACCTGAAA AAACCACAGC TGTACCAAAA
    GGCAGAGCTA CTAATTCTCA AGTGACAACT CCTAAACCGC AGAAACCAAC CAAAGCACCC
    AAAAAGCCCA CTTCGACCAA AAAGCCAAGA ACACCTAGAG TGAGAAAACC AAAGACTACG
    CCAACGCCCC CAAAGACGAC GACATCAGCA ATGCCTGAAC CGACTCCTAC CTCTTTACCA
    GAAGCCATGC TCCAAACCAC CACCAGGCCT ACCCCAACCC CCAACTCAGA AATAATTGAC
    GTAAACCCAG AGAATGAGGA TGGAGATGCT GCTGAAGGAG AAAAACCTCA CATGATTTTC
    AGGCCCCCTG TGCTAACTCC TATAGTGATT CCAGGCACTG AAATCATAGT GAGAGGACCC
    AGTCAAGGCT TCGGCATCAA CCCCATGTTT TCAGATGAAA CTAATCTATG CAACGGTAGG
    CCAGTAGATG GACTGACTAC TTTGCGTAAT GGGACATTAG TTGCATTTCG AGGTCATTAT
    TTCTGGATGC TGACTCCATT TACTCCACCA CCTCCACCTC GGAGAATTAC TGAAGTTTGG
    GGCATTCCCT CCCCCATTGA TACTGTTTTT ACTAGATGCA ACTGTGAAGG AAAAACTTTC
    TTCTTTAAGG GTTCTCAGTA CTGGCGTTTC ACCAATGATA TAAAAGATGC AGGATATCCC
    AAACTAATTT CCAAAGGATT TGGAGGACTG AATGGAAAAA TAGTGGCAGC CCTCTCAATA
    GCTCAGTACA AGAGCAGACC TGAATCCGTG TATTTTTTCA AGAGAGGTGG CAGCGTTCAG
    CAATACACTT ACAAACAGGA ACCCACCCAA AAGTGTACTG GAAGAAGGCC TGCGATAAAC
    TATTCTGTGT ATGGAGAAAC AGCACAGGTT AGGAGACGTC GATTTGAACG TGCTATAGGA
    CCTTCTCAAG TACACACCAT CAGGATTCAC TACACACCCG TCAGAGTCCC TTATCAAGAC
    AAAGGTTTCC TCCATAATGA AGTTAAAGTG AGTACACTGT GGAGAGGACT TCCAAATGTG
    GTTACTTCAG CAATATCACT GCCCAACATC AGAAAACCCG ATGGCTATGA TTACTATGCC
    CTTTCTAAAG ATCAATACTA TAACATCGAC GTGCCAAGTA GGACAGCAAG AGCCATTACT
    ACTCGTTCTG GGCAGACCTT ATCCAATACC TGGTACAACT GTCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193562.1
    CDS22..3609
    Translation

    Target ORF information:

    RefSeq Version NM_001206633.1
    Organism Bos taurus(cattle)
    Definition Bos taurus proteoglycan 4 (PRG4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGTGGA AAATACTTCC CATTTACTTG CTGCTGTTTT CTGTTTTCTT GATTCAGCAA 
    GCTTCTTCTC AAGATTTATC AAGCTGTGCA GGGAGATGTG GGGAAGGGTA TTCTAGAGAT
    GCCATCTGCA ACTGTGACTA TAACTGTCAA CACTACACGG AGTGCTGCCC TGACTTCAAG
    AAGGAATGCA CTGTGGAGCT TTCCTGTAAA GGCCGCTGCT TCGAAACCTT TGCAAGAGGG
    AGGGAGTGTG ACTGCGACTC AGACTGCAAG AAGTATGGCA AGTGCTGTTC CGATTATGGC
    ACTTTTTGTG AAGCAGTGCA TAACCCCACA ACACCACCTC CTCCAAAGAC TGCACCTCCA
    CCTCCAGGAG CATCTCAGAC CATCAAGTCA ACAACCAAAC GTTCACCCAA ATCACCAAAC
    AAGAAGAAGA TTAAGAAAGT TATAGAATCA GAGGAAATAA CAGAAGAACA TTCTGTTTCT
    GAAAATCAAG AGTCTTCCTC CTCCTCTTCC TCTTCCTCTT CAACTATTCG GAAAATCAAG
    TCTTCCAAAA ATTCAGCTGC TAATAGAGAA TTAAAGAAGA AACCCAAAGT AAAAGATAAG
    AAGAAAAGAA CTCCTAAAAA TAAACCTACC CCAGAACCAC CAGTTATAGA TGAAGATGGA
    AGTGGACTGG ATAATGGTGA TTTCAAGCTC ACCCCAACTC CTAACATTCC CACCACACAA
    CGTAATAAAG TGACCACAAC TCCCAAGATT ACAACAGTAA AACCAACACT TCCTAAGCCC
    AGTCTTCCAC CTAATTCTGA TACAACTAAA GAGATACCTT CGACAGTCAA CAGAGAGACA
    ACAGTTGAAA CTAAAGAGAC TTCTACAACA AATAAACCGA CTTCCACTAG CGCAAAAGAG
    AAGACAACTT CAGCTAAAGA GACACGAACT GCAGAGAATA CATCTACTAA AGGTCTTGCA
    CCCACACCTG AAGTTCCTGC TATATCTACA CCCAAAGCTG AAACTATAAC CAGATCCCCT
    ACACCCACCA CCCCCAAGGA TCCTCCTCCC ACACACCCCA AGGAGCCTGC TCCCACCAAC
    AACAAGGAGC CTACTCCCAC TACTCCCAAG GAGCCTGCTC CCACCACCAC CCCCAAGGAG
    CCTGCACCCA CCACCAAGGA GCCTGAACCC ACCACCAAGG AGCCTGCACC CACTACCACC
    ACCAAGGAGC CTGCACCCAC CATCCCCAAG GAACCTGCAC CCACCACCCC CAAAGAGCCT
    GCTCCCACCA CCCACAAGGA GCCTACACCC ACCACCACCC CCAAAGAGCC TGCTCCCACC
    AGCCCCAAGG AACCTGCTCC CACCAGCCCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCCC CAAGGAGCCT GCTCCCACCG ATCCCAAGGA GCCTGCTCCC
    GCTGAACCCA AGGAGCCTGC ACCCACCAGC TCCAAGGAGC CTGCACCCAC TACCACCACC
    AAGGAGCCTG CACCCACTAC CACCCCCAAG GAGCCTGCTC CGACCGATCC CAAGGAGCCT
    GCTCCCGCTG AACCCAAGGA GCCTGCACCC ACCAGCCCCA AGGAGCCTGC ACCCACTACC
    ACCACCAAGG AGCCTGCTCC CGCTGAACCC AAGGAGCCTG CACCCACCAG CCCCAAGGAG
    CCTGCACCCA CTACCACCAC CAAGGAGCCT GCTCCCGCTG AACCCAAGGA GCCTGCACCC
    ACCAGCCCCA AGGAGCCTGC ACCCACTACC ACCACCAAGG AGCCTGCTCC CACTACCACC
    CCCAAGGAGC CTGCTCCGAC CGATCCCAAG GAGCCTGCTC CCGCTGAACC CAAGGAGCCA
    GCTCCTGCTG AACCCAAGGA GCCCACTCCA AATTCTCCTG AGACACTTGC TCCAAGTTCT
    CCTGATATCC CTGCTCCAAC CACCTCAGAG GCCTCTACTT CAACTACCAC CGTGGAGCCT
    TCCACTACTC TGAAGAGCCC TGCTGAATCA ACTCCTCAAC CTCCTATAGA ACCCACACCA
    AAGGCTCTTG AAAACAGTCC CAAGGAACCT GCAGTACCCA CAATCAAGGG TCCTAAAGTG
    AACAAACCTG AAATAACTAC AACAGTTAAA GACAAGTCCA CAGAAAAAGA CATATATTCT
    ACACCTGAAA TTACAACAGC TGTAGCTAAG ATTATAACAG AGGCAGCAAC TAAAACAGAA
    AAGACTACTG AATCGAAAAT ACCAAGTACA ACCACAGATG TAACATCTAC CACAACCAAA
    GATACCACGT CATCCAAAAT TACTCCTAAA GCAACTCTTG CACCTAAAGT AATGACTGCA
    ACAAAGAAGA CGACCGAAAA GACTAAGAAC AAACCTGAAA AAACCACAGC TGTACCAAAA
    GGCAGAGCTA CTAATTCTCA AGTGACAACT CCTAAACCGC AGAAACCAAC CAAAGCACCC
    AAAAAGCCCA CTTCGACCAA AAAGCCAAGA ACACCTAGAG TGAGAAAACC AAAGACTACG
    CCAACGCCCC CAAAGACGAC GACATCAGCA ATGCCTGAAC CGACTCCTAC CTCTTTACCA
    GAAGCCATGC TCCAAACCAC CACCAGGCCT ACCCCAACCC CCAACTCAGA AATAATTGAC
    GTAAACCCAG AGAATGAGGA TGGAGATGCT GCTGAAGGAG AAAAACCTCA CATGATTTTC
    AGGCCCCCTG TGCTAACTCC TATAGTGATT CCAGGCACTG AAATCATAGT GAGAGGACCC
    AGTCAAGGCT TCGGCATCAA CCCCATGTTT TCAGATGAAA CTAATCTATG CAACGGTAGG
    CCAGTAGATG GACTGACTAC TTTGCGTAAT GGGACATTAG TTGCATTTCG AGGTCATTAT
    TTCTGGATGC TGACTCCATT TACTCCACCA CCTCCACCTC GGAGAATTAC TGAAGTTTGG
    GGCATTCCCT CCCCCATTGA TACTGTTTTT ACTAGATGCA ACTGTGAAGG AAAAACTTTC
    TTCTTTAAGG GTTCTCAGTA CTGGCGTTTC ACCAATGATA TAAAAGATGC AGGATATCCC
    AAACTAATTT CCAAAGGATT TGGAGGACTG AATGGAAAAA TAGTGGCAGC CCTCTCAATA
    GCTCAGTACA AGAGCAGACC TGAATCCGTG TATTTTTTCA AGAGAGGTGG CAGCGTTCAG
    CAATACACTT ACAAACAGGA ACCCACCCAA AAGTGTACTG GAAGAAGGCC TGCGATAAAC
    TATTCTGTGT ATGGAGAAAC AGCACAGGTT AGGAGACGTC GATTTGAACG TGCTATAGGA
    CCTTCTCAAG TACACACCAT CAGGATTCAC TACACACCCG TCAGAGTCCC TTATCAAGAC
    AAAGGTTTCC TCCATAATGA AGTTAAAGTG AGTACACTGT GGAGAGGACT TCCAAATGTG
    GTTACTTCAG CAATATCACT GCCCAACATC AGAAAACCCG ATGGCTATGA TTACTATGCC
    CTTTCTAAAG ATCAATACTA TAACATCGAC GTGCCAAGTA GGACAGCAAG AGCCATTACT
    ACTCGTTCTG GGCAGACCTT ATCCAATACC TGGTACAACT GTCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.