MET cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MET
Entrez Gene ID	280855
Full Name	MET proto-oncogene, receptor tyrosine kinase
Synonyms	HGFR,c-met
General protein information	Preferred Names MET proto-oncogene, receptor tyrosine kinase Names hepatocyte growth factor receptor HGF receptor c-Met HGF/SF receptor SF receptor met proto-oncogene (hepatocyte growth factor receptor) met proto-oncogene tyrosine kinase proto-oncogene c-Met scatter factor receptor tyrosine-protein kinase Met
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024990409.1	XP_024846177.1	hepatocyte growth factor receptor isoform X3
XM_024990408.1	XP_024846176.1	hepatocyte growth factor receptor isoform X2
NM_001012999.2	NP_001013017.2	hepatocyte growth factor receptor precursor
XM_024990407.1	XP_024846175.1	hepatocyte growth factor receptor isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MET	NP_001002963.1
Rattus norvegicus (Norway rat)	Met	NP_113705.1
Gallus gallus (chicken)	MET	NP_990543.1
Homo sapiens (human)	MET	NP_001120972.1
Pan troglodytes (chimpanzee)	MET	NP_001129302.1
Bos taurus (cattle)	MET	NP_001013017.2
Mus musculus (house mouse)	Met	NP_032617.2
Danio rerio (zebrafish)	met	NP_001007125.1
Xenopus tropicalis (tropical clawed frog)	met	XP_002935001.1
	MET	NP_001162100.1

Gene Ontology
Bibliography

Related articles in PubMed

Epidermal growth factor and hepatocyte growth factor receptors collaborate to induce multiple biological responses in bovine mammary epithelial cells.
Accornero P, Martignani E, Miretti S, Starvaggi Cucuzza L, Baratta M
Journal of dairy science92(8)3667-75(2009 Aug)

Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland.
Yamaji D, Kimura K, Watanabe A, Kon Y, Iwanaga T, Soliman MM, Ahmed MM, Saito M
Domestic animal endocrinology30(3)239-46(2006 Mar)

Comparative analyses of multi-species sequences from targeted genomic regions.
Thomas JW, Touchman JW, Blakesley RW, Bouffard GG, Beckstrom-Sternberg SM, Margulies EH, Blanchette M, Siepel AC, Thomas PJ, McDowell JC, Maskeri B, Hansen NF, Schwartz MS, Weber RJ, Kent WJ, Karolchik D, Bruen TC, Bevan R, Cutler DJ, Schwartz S, Elnitski L, Idol JR, Prasad AB, Lee-Lin SQ, Maduro VV, Summers TJ, Portnoy ME, Dietrich NL, Akhter N, Ayele K, Benjamin B, Cariaga K, Brinkley CP, Brooks SY, Granite S, Guan X, Gupta J, Haghighi P, Ho SL, Huang MC, Karlins E, Laric PL, Legaspi R, Lim MJ, Maduro QL, Masiello CA, Mastrian SD, McCloskey JC, Pearson R, Stantripop S, Tiongson EE, Tran JT, Tsurgeon C, Vogt JL, Walker MA, Wetherby KD, Wiggins LS, Young AC, Zhang LH, Osoegawa K, Zhu B, Zhao B, Shu CL, De Jong PJ, Lawrence CE, Smit AF, Chakravarti A, Haussler D, Green P, Miller W, Green ED
Nature424(6950)788-93(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

possible cooperative role of the EGF and HGF pathways and indicate that cross-talk between their respective receptors may modulate mammary gland development in the cow
Title: Epidermal growth factor and hepatocyte growth factor receptors collaborate to induce multiple biological responses in bovine mammary epithelial cells.

The following MET gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MET cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233521	XM_024990409.1 Latest version!	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa233519	XM_024990407.1 Latest version!	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa233520	XM_024990408.1 Latest version!	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa41548	NM_001012999.2 Latest version!	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MET ( XM_024990409.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846177.1 Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa233521
Clone ID Related Accession (Same CDS sequence)	XM_024990409.1
Accession Version	XM_024990409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hepatocyte growth factor receptor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTTG TGGTTTCTCG ATCAGGATCA TTAACTCCTC ATGTGAATTT TCACCTGGAT 
TCCCACCCTG TGTCTCCAGA AGTGATTGTG GAGCACCCGC TAAACCAGAA TGGCTATACA 
CTGGTTGTCA CTGGGAAGAA GATCACCAAG ATCCCGCTGA ATGGCCTGGG CTGTGAACAT 
TTCCAGTCCT GCAGTCAGTG CCTCTCAGCC CCTTCCTTTG TGCAATGTGG CTGGTGCCAT 
GATAAATGTG TGCGATTGGA GGAATGCTCC AGTGGAACGT GGACTCAAGA GACCTGTCTG 
CCAACAATCT ACAAGGTTTT TCCAACTAGT GCCCCCCTTG AAGGAGGGAC AACACTGACT 
GTTTGTGGCT GGGACTTTGG ATTCAAGAGG AATAATAAAT TTGACTTGAA GAAAACCAGA 
GTTCTCCTTG GCAATGAGAG CTGCACCTTA ACCTTAACTG AGAGCACAAC AAACATGTTG 
AAATGCACAG TTGGTCCTGC AATGAATGAA CATTTCAATA TGTCCATAGT TATTTCAAAC 
AGCCGTGGGT CAGTAGAGTA TAGTGCGTTT TCCTATGTGG ATCCTATAAT AACAAGTATT 
TCTCCAAATT ATGGTCCAAA AACTGGTGGC ACTTTACTTA CATTAACTGG AAAGCACCTA 
AACAGTGGAA ATTCAAGACA CATTTCAATT GGTGGAAAAA CATGTACTTT AAAAAGTGTT 
TCACATAGTA TTCTCGAATG TTATACCCCA GCCCAAAGTG CTCCAACTGA GTTTTCTGTT 
AAGTTGAAAA TTGACTTAGC CAACCGAGAG GTGAACAGCT TCATTTACCG GGAAGACCCC 
ATTGTCTATG AAATACATCC CACCAAATCT TTTATTAGTG GTGGGAGTAC AATAACAGGT 
GTTGGAAAAA ACCTGAATTC AGTTAGTGTC CTAAGGATGG TAATAAATGT GCATGAAGCC 
GGAAGGAACT TTACAGTGGC ATGTCAACAT CGCTCTAATT CAGAGATAAT CTGCTGTACA 
ACTCCTTCAA TAGAACAGCT GAACCTACAA CTCCCCCTGA AAACCAAAGC CTTTTTCATG 
TTAGATGGGA TCCATTCCAA ATACTTTGAT CTCATTTATG TACATAATCC TGTGTTTAAG 
CCTTTTGAAA AGCCAGTGAT GATCTCAGTA GGCAATGAAA ATGTACTGGA AATTAAGGGA 
AATGATATTG ACCCTGAAGC AGTTAAAGGG GAGGTGTTAA AAGTTGGAAA TAAGAGCTGT 
GAAAATATAC ACTCACACTC TGAAGCTGTT TTATGTACGG TCCCCAGTGA CCTGCTGAAA 
TTGAACAGCG AACTAAATAT AGAGTGGAAG CAAGCAATTT CTTCAACTGT TCTTGGAAAA 
GTGATAGTTC AACCAGATCA GAATTTCACA GGATTGATTG TTGGTGTTGT CTCAATATCA 
ATAATACTCT TACTGTTACT CGGGCTTTTC CTGTGGCTGA AAAAAAGGAA GCAAATTAAA 
GATCTGGGCA GTGAATTAGT TCGCTATGAT GCAAGAGTAC ACACTCCACA TTTGGATAGG 
CTTGTCAGTG CCCGAAGTGT AAGCCCAACT ACAGAAATGG TTTCCAATGA ATCTGTAGAC 
TACCGAGCTA CTTTTCCAGA AGACCAGTTT CCTAATGCAT CTCAGAATGG GTCATGCAGA 
CAAGTGCAGT ATCCTCTGAC AGATCTATCC CCCATCTTAA CTAGTGGGGA CTCTGATATA 
TCCAGTCCAT TACTGCAAAA TACCGTCCAC ATCGACCTCA GTGCTCTAAA TCCAGAGCTG 
GTCCAGGCAG TTCAGCACGT AGTAATTGGG CCGAGCAGTC TGATTGTACA TTTCAATGAA 
GTCATAGGAA GAGGACATTT TGGGTGTGTA TATCATGGAA CTTTGTTGGA CAACGATGAT 
AAGAAAATTC ACTGTGCTGT GAAATCCTTG AATAGAATCA CTGACATAGG AGAAGTTTCC 
CAATTTCTGA CTGAGGGAAT CATCATGAAA GATTTTAGTC ATCCCAATGT TCTCTCACTT 
TTGGGAATCT GCCTTCGAAG TGAGGGGTCT CCACTGGTGG TCCTACCATA CATGAAACAC 
GGGGATCTTC GAAATTTCAT TAGAAATGAG ACTCATAACC CAACTGTAAA AGATCTTATT 
GGCTTTGGTC TTCAAGTAGC CAAAGGCATG GAATATCTTG CAAGCAAAAA GTTTGTCCAC 
AGAGACTTGG CTGCAAGAAA CTGTATGCTG GATGAAAAAT TCACCGTCAA GGTTGCTGAT 
TTTGGTCTTG CCAGAGACGT GTATGATAAG GAATACTATA GCGTACACAA CAAAACAGGT 
GCAAAACTGC CAGTGAAATG GATGGCTTTA GAAAGCCTGC AGACTCAGAA ATTTACCACC 
AAGTCAGATG TGTGGTCCTT TGGCGTGCTT CTCTGGGAAC TGATGACAAG AGGAGCACCA 
CCTTATCCTG ACGTCAACAC CTTTGACATA ACAGTTTACT TGTTGCAAGG AAGAAGGCTC 
CTACAACCTG AATACTGCCC AGATCCCTTG TATGAAGTGA TGCTGAAATG CTGGCACCCT 
AAAGCTGAAC TGCGCCCATC CTTTTCTGAA CTGGTCTCCA GGATATCCGT GATATTCTCT 
ACTTTCATTG GGGAGCACTA TGTCCATGTG AATGCTACTT ACGTGAACGT CAAATGTGTC 
GCTCCATATC CTTCTCTGTT GTCATCACAA GATAATGTCA GTGGCGAGGA TGATGATGAC 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846177.1
CDS	418..3183
Translation	MQVVVSRSGSLTPHVNFHLDSHPVSPEVIVEHPLNQNGYTLVVTGKKITKIPLNGLGCEHFQSCSQCLSAPSFVQCGWCHDKCVRLEECSSGTWTQETCLPTIYKVFPTSAPLEGGTTLTVCGWDFGFKRNNKFDLKKTRVLLGNESCTLTLTESTTNMLKCTVGPAMNEHFNMSIVISNSRGSVEYSAFSYVDPIITSISPNYGPKTGGTLLTLTGKHLNSGNSRHISIGGKTCTLKSVSHSILECYTPAQSAPTEFSVKLKIDLANREVNSFIYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVLRMVINVHEAGRNFTVACQHRSNSEIICCTTPSIEQLNLQLPLKTKAFFMLDGIHSKYFDLIYVHNPVFKPFEKPVMISVGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHSHSEAVLCTVPSDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTGLIVGVVSISIILLLLLGLFLWLKKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNASQNGSCRQVQYPLTDLSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDDKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMEYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDVYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAELRPSFSELVSRISVIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSQDNVSGEDDDDT

Target ORF information:

RefSeq Version	XM_024990409.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990409.1

ORF Insert Sequence:

ATGCAGGTTG TGGTTTCTCG ATCAGGATCA TTAACTCCTC ATGTGAATTT TCACCTGGAT 
TCCCACCCTG TGTCTCCAGA AGTGATTGTG GAGCACCCGC TAAACCAGAA TGGCTATACA 
CTGGTTGTCA CTGGGAAGAA GATCACCAAG ATCCCGCTGA ATGGCCTGGG CTGTGAACAT 
TTCCAGTCCT GCAGTCAGTG CCTCTCAGCC CCTTCCTTTG TGCAATGTGG CTGGTGCCAT 
GATAAATGTG TGCGATTGGA GGAATGCTCC AGTGGAACGT GGACTCAAGA GACCTGTCTG 
CCAACAATCT ACAAGGTTTT TCCAACTAGT GCCCCCCTTG AAGGAGGGAC AACACTGACT 
GTTTGTGGCT GGGACTTTGG ATTCAAGAGG AATAATAAAT TTGACTTGAA GAAAACCAGA 
GTTCTCCTTG GCAATGAGAG CTGCACCTTA ACCTTAACTG AGAGCACAAC AAACATGTTG 
AAATGCACAG TTGGTCCTGC AATGAATGAA CATTTCAATA TGTCCATAGT TATTTCAAAC 
AGCCGTGGGT CAGTAGAGTA TAGTGCGTTT TCCTATGTGG ATCCTATAAT AACAAGTATT 
TCTCCAAATT ATGGTCCAAA AACTGGTGGC ACTTTACTTA CATTAACTGG AAAGCACCTA 
AACAGTGGAA ATTCAAGACA CATTTCAATT GGTGGAAAAA CATGTACTTT AAAAAGTGTT 
TCACATAGTA TTCTCGAATG TTATACCCCA GCCCAAAGTG CTCCAACTGA GTTTTCTGTT 
AAGTTGAAAA TTGACTTAGC CAACCGAGAG GTGAACAGCT TCATTTACCG GGAAGACCCC 
ATTGTCTATG AAATACATCC CACCAAATCT TTTATTAGTG GTGGGAGTAC AATAACAGGT 
GTTGGAAAAA ACCTGAATTC AGTTAGTGTC CTAAGGATGG TAATAAATGT GCATGAAGCC 
GGAAGGAACT TTACAGTGGC ATGTCAACAT CGCTCTAATT CAGAGATAAT CTGCTGTACA 
ACTCCTTCAA TAGAACAGCT GAACCTACAA CTCCCCCTGA AAACCAAAGC CTTTTTCATG 
TTAGATGGGA TCCATTCCAA ATACTTTGAT CTCATTTATG TACATAATCC TGTGTTTAAG 
CCTTTTGAAA AGCCAGTGAT GATCTCAGTA GGCAATGAAA ATGTACTGGA AATTAAGGGA 
AATGATATTG ACCCTGAAGC AGTTAAAGGG GAGGTGTTAA AAGTTGGAAA TAAGAGCTGT 
GAAAATATAC ACTCACACTC TGAAGCTGTT TTATGTACGG TCCCCAGTGA CCTGCTGAAA 
TTGAACAGCG AACTAAATAT AGAGTGGAAG CAAGCAATTT CTTCAACTGT TCTTGGAAAA 
GTGATAGTTC AACCAGATCA GAATTTCACA GGATTGATTG TTGGTGTTGT CTCAATATCA 
ATAATACTCT TACTGTTACT CGGGCTTTTC CTGTGGCTGA AAAAAAGGAA GCAAATTAAA 
GATCTGGGCA GTGAATTAGT TCGCTATGAT GCAAGAGTAC ACACTCCACA TTTGGATAGG 
CTTGTCAGTG CCCGAAGTGT AAGCCCAACT ACAGAAATGG TTTCCAATGA ATCTGTAGAC 
TACCGAGCTA CTTTTCCAGA AGACCAGTTT CCTAATGCAT CTCAGAATGG GTCATGCAGA 
CAAGTGCAGT ATCCTCTGAC AGATCTATCC CCCATCTTAA CTAGTGGGGA CTCTGATATA 
TCCAGTCCAT TACTGCAAAA TACCGTCCAC ATCGACCTCA GTGCTCTAAA TCCAGAGCTG 
GTCCAGGCAG TTCAGCACGT AGTAATTGGG CCGAGCAGTC TGATTGTACA TTTCAATGAA 
GTCATAGGAA GAGGACATTT TGGGTGTGTA TATCATGGAA CTTTGTTGGA CAACGATGAT 
AAGAAAATTC ACTGTGCTGT GAAATCCTTG AATAGAATCA CTGACATAGG AGAAGTTTCC 
CAATTTCTGA CTGAGGGAAT CATCATGAAA GATTTTAGTC ATCCCAATGT TCTCTCACTT 
TTGGGAATCT GCCTTCGAAG TGAGGGGTCT CCACTGGTGG TCCTACCATA CATGAAACAC 
GGGGATCTTC GAAATTTCAT TAGAAATGAG ACTCATAACC CAACTGTAAA AGATCTTATT 
GGCTTTGGTC TTCAAGTAGC CAAAGGCATG GAATATCTTG CAAGCAAAAA GTTTGTCCAC 
AGAGACTTGG CTGCAAGAAA CTGTATGCTG GATGAAAAAT TCACCGTCAA GGTTGCTGAT 
TTTGGTCTTG CCAGAGACGT GTATGATAAG GAATACTATA GCGTACACAA CAAAACAGGT 
GCAAAACTGC CAGTGAAATG GATGGCTTTA GAAAGCCTGC AGACTCAGAA ATTTACCACC 
AAGTCAGATG TGTGGTCCTT TGGCGTGCTT CTCTGGGAAC TGATGACAAG AGGAGCACCA 
CCTTATCCTG ACGTCAACAC CTTTGACATA ACAGTTTACT TGTTGCAAGG AAGAAGGCTC 
CTACAACCTG AATACTGCCC AGATCCCTTG TATGAAGTGA TGCTGAAATG CTGGCACCCT 
AAAGCTGAAC TGCGCCCATC CTTTTCTGAA CTGGTCTCCA GGATATCCGT GATATTCTCT 
ACTTTCATTG GGGAGCACTA TGTCCATGTG AATGCTACTT ACGTGAACGT CAAATGTGTC 
GCTCCATATC CTTCTCTGTT GTCATCACAA GATAATGTCA GTGGCGAGGA TGATGATGAC 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MET ( XM_024990407.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846175.1 Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233519
Clone ID Related Accession (Same CDS sequence)	XM_024990407.1
Accession Version	XM_024990407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hepatocyte growth factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCC CTGCTGTGCT TGCACCTGGC ATTCTTGTGC TTCTGTTTAC CTTCGTGCAG 
AAGAGCAATG GGGAGTGTAA AGAGGCACTA GTAAAATCCA GGATGAATGT GAACATGCAA 
TATCAGCTTC CTAACTTCAC TGCAGAAACA TCCATTCAGA ATGTTGTTTT GCACAAACAT 
CACATTTACC TCGGTGCCAT TAACTACATT TACGTTTTAA ATGACAAAGA CCTTCAGAAG 
GTTGCTGAGT ACAAGACTGG GCCTGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC 
TGCAGCCATA AAGCCAATTT ATCAGGTGGT GTTTGGAAAG ATAACATCAA CATGGCTCTG 
CTTGTTGACA CGTACTATGA TGATCAACTC ATTAGCTGTG GCAGTGTCCA CAGAGGGACC 
TGCCAGCGAC ACGTCCTTCC ACCCAACAAT ACTGCAGACA TAGAGTCGGA GGTTCACTGC 
ATGTACTCTC CTCAGGCAGA CGAAGAGACC AACCAGTGTC CTGACTGTGT GGTGAGTGCC 
CTGGGAACCA AAGTCCTACT GTCTGAAAAG GATCGATTCA TCAACTTCTT CGTGGGCAAT 
ACCATAAATT CTTCTTACCT TCCAGATTAT ATATTGCACT CGATATCAGT GAGAAGGCTA 
AAGGAAACAC AAGATGGTTT TAAGTTTTTG ACAGACCAAT CCTATATTGA TGTTCTACCT 
GAACTCCGAG ATTCCTACCC CATTAAGTAT GTCCACGCCT TTGAAAGCAA CCATTTTATT 
TACTTTTTGA CGGTCCAGCG GGAAACTCTA GATGCTCAGA CTTTTCACAC AAGAATAATC 
AGGTTCTGTT CCGCAGACTC TGGATTGCAT TCGTACATGG AAATGCCTCT GGAGTGTATT 
CTGACGGAAA AGAGAAGAAA GAGATCCACA AAGCAGGAAG TGTTTAATAT TCTCCAAGCT 
GCATATGTCA GTAAGCCTGG GGCTCAGCTC GCTAGGCAAA TAGGTGCCAG CCTGAATGAT 
GACATTCTGT ACGGGGTGTT TGCACAAAGC AAGCCAGATT CCTCAGAACC AATGAATCGT 
TCTGCTGTCT GTGCATTCCC TGTCAAATAT GTCAATGAGT TCTTCAACAA AATCGTCAAC 
AAAAACAATG TGAGATGTCT CCAGCACTTT TATGGCCCCA ACCATGAACA CTGCTTCAAT 
AGGACACTTT TGAGAAATTC ATCAGGATGT GAAGTGCGCA ATGATGAATA TCGAACGGAG 
TTTACCACAG CTTTGCCACG CGTTGACTTA TTCACGGGCC AGTTCAACCA AGTCCTCTTA 
ACATCTATAT CCACCTTTAT CAAAGGAGAT CTCACCATCG CTAATCTCGG GACATCAGAA 
GGTCGATTCA TGCAGGTTGT GGTTTCTCGA TCAGGATCAT TAACTCCTCA TGTGAATTTT 
CACCTGGATT CCCACCCTGT GTCTCCAGAA GTGATTGTGG AGCACCCGCT AAACCAGAAT 
GGCTATACAC TGGTTGTCAC TGGGAAGAAG ATCACCAAGA TCCCGCTGAA TGGCCTGGGC 
TGTGAACATT TCCAGTCCTG CAGTCAGTGC CTCTCAGCCC CTTCCTTTGT GCAATGTGGC 
TGGTGCCATG ATAAATGTGT GCGATTGGAG GAATGCTCCA GTGGAACGTG GACTCAAGAG 
ACCTGTCTGC CAACAATCTA CAAGGTTTTT CCAACTAGTG CCCCCCTTGA AGGAGGGACA 
ACACTGACTG TTTGTGGCTG GGACTTTGGA TTCAAGAGGA ATAATAAATT TGACTTGAAG 
AAAACCAGAG TTCTCCTTGG CAATGAGAGC TGCACCTTAA CCTTAACTGA GAGCACAACA 
AACATGTTGA AATGCACAGT TGGTCCTGCA ATGAATGAAC ATTTCAATAT GTCCATAGTT 
ATTTCAAACA GCCGTGGGTC AGTAGAGTAT AGTGCGTTTT CCTATGTGGA TCCTATAATA 
ACAAGTATTT CTCCAAATTA TGGTCCAAAA ACTGGTGGCA CTTTACTTAC ATTAACTGGA 
AAGCACCTAA ACAGTGGAAA TTCAAGACAC ATTTCAATTG GTGGAAAAAC ATGTACTTTA 
AAAAGTGTTT CACATAGTAT TCTCGAATGT TATACCCCAG CCCAAAGTGC TCCAACTGAG 
TTTTCTGTTA AGTTGAAAAT TGACTTAGCC AACCGAGAGG TGAACAGCTT CATTTACCGG 
GAAGACCCCA TTGTCTATGA AATACATCCC ACCAAATCTT TTATTAGTGG TGGGAGTACA 
ATAACAGGTG TTGGAAAAAA CCTGAATTCA GTTAGTGTCC TAAGGATGGT AATAAATGTG 
CATGAAGCCG GAAGGAACTT TACAGTGGCA TGTCAACATC GCTCTAATTC AGAGATAATC 
TGCTGTACAA CTCCTTCAAT AGAACAGCTG AACCTACAAC TCCCCCTGAA AACCAAAGCC 
TTTTTCATGT TAGATGGGAT CCATTCCAAA TACTTTGATC TCATTTATGT ACATAATCCT 
GTGTTTAAGC CTTTTGAAAA GCCAGTGATG ATCTCAGTAG GCAATGAAAA TGTACTGGAA 
ATTAAGGGAA ATGATATTGA CCCTGAAGCA GTTAAAGGGG AGGTGTTAAA AGTTGGAAAT 
AAGAGCTGTG AAAATATACA CTCACACTCT GAAGCTGTTT TATGTACGGT CCCCAGTGAC 
CTGCTGAAAT TGAACAGCGA ACTAAATATA GAGTGGAAGC AAGCAATTTC TTCAACTGTT 
CTTGGAAAAG TGATAGTTCA ACCAGATCAG AATTTCACAG GATTGATTGT TGGTGTTGTC 
TCAATATCAA TAATACTCTT ACTGTTACTC GGGCTTTTCC TGTGGCTGAA AAAAAGGAAG 
CAAATTAAAG ATCTGGGCAG TGAATTAGTT CGCTATGATG CAAGAGTACA CACTCCACAT 
TTGGATAGGC TTGTCAGTGC CCGAAGTGTA AGCCCAACTA CAGAAATGGT TTCCAATGAA 
TCTGTAGACT ACCGAGCTAC TTTTCCAGAA GACCAGTTTC CTAATGCATC TCAGAATGGG 
TCATGCAGAC AAGTGCAGTA TCCTCTGACA GATCTATCCC CCATCTTAAC TAGTGGGGAC 
TCTGATATAT CCAGTCCATT ACTGCAAAAT ACCGTCCACA TCGACCTCAG TGCTCTAAAT 
CCAGAGCTGG TCCAGGCAGT TCAGCACGTA GTAATTGGGC CGAGCAGTCT GATTGTACAT 
TTCAATGAAG TCATAGGAAG AGGACATTTT GGGTGTGTAT ATCATGGAAC TTTGTTGGAC 
AACGATGATA AGAAAATTCA CTGTGCTGTG AAATCCTTGA ATAGAATCAC TGACATAGGA 
GAAGTTTCCC AATTTCTGAC TGAGGGAATC ATCATGAAAG ATTTTAGTCA TCCCAATGTT 
CTCTCACTTT TGGGAATCTG CCTTCGAAGT GAGGGGTCTC CACTGGTGGT CCTACCATAC 
ATGAAACACG GGGATCTTCG AAATTTCATT AGAAATGAGA CTCATAACCC AACTGTAAAA 
GATCTTATTG GCTTTGGTCT TCAAGTAGCC AAAGGCATGG AATATCTTGC AAGCAAAAAG 
TTTGTCCACA GAGACTTGGC TGCAAGAAAC TGTATGCTGG ATGAAAAATT CACCGTCAAG 
GTTGCTGATT TTGGTCTTGC CAGAGACGTG TATGATAAGG AATACTATAG CGTACACAAC 
AAAACAGGTG CAAAACTGCC AGTGAAATGG ATGGCTTTAG AAAGCCTGCA GACTCAGAAA 
TTTACCACCA AGTCAGATGT GTGGTCCTTT GGCGTGCTTC TCTGGGAACT GATGACAAGA 
GGAGCACCAC CTTATCCTGA CGTCAACACC TTTGACATAA CAGTTTACTT GTTGCAAGGA 
AGAAGGCTCC TACAACCTGA ATACTGCCCA GATCCCTTGT ATGAAGTGAT GCTGAAATGC 
TGGCACCCTA AAGCTGAACT GCGCCCATCC TTTTCTGAAC TGGTCTCCAG GATATCCGTG 
ATATTCTCTA CTTTCATTGG GGAGCACTAT GTCCATGTGA ATGCTACTTA CGTGAACGTC 
AAATGTGTCG CTCCATATCC TTCTCTGTTG TCATCACAAG ATAATGTCAG TGGCGAGGAT 
GATGATGACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846175.1
CDS	143..4297
Translation	MKAPAVLAPGILVLLFTFVQKSNGECKEALVKSRMNVNMQYQLPNFTAETSIQNVVLHKHHIYLGAINYIYVLNDKDLQKVAEYKTGPVLEHPDCFPCQDCSHKANLSGGVWKDNINMALLVDTYYDDQLISCGSVHRGTCQRHVLPPNNTADIESEVHCMYSPQADEETNQCPDCVVSALGTKVLLSEKDRFINFFVGNTINSSYLPDYILHSISVRRLKETQDGFKFLTDQSYIDVLPELRDSYPIKYVHAFESNHFIYFLTVQRETLDAQTFHTRIIRFCSADSGLHSYMEMPLECILTEKRRKRSTKQEVFNILQAAYVSKPGAQLARQIGASLNDDILYGVFAQSKPDSSEPMNRSAVCAFPVKYVNEFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEVRNDEYRTEFTTALPRVDLFTGQFNQVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGSLTPHVNFHLDSHPVSPEVIVEHPLNQNGYTLVVTGKKITKIPLNGLGCEHFQSCSQCLSAPSFVQCGWCHDKCVRLEECSSGTWTQETCLPTIYKVFPTSAPLEGGTTLTVCGWDFGFKRNNKFDLKKTRVLLGNESCTLTLTESTTNMLKCTVGPAMNEHFNMSIVISNSRGSVEYSAFSYVDPIITSISPNYGPKTGGTLLTLTGKHLNSGNSRHISIGGKTCTLKSVSHSILECYTPAQSAPTEFSVKLKIDLANREVNSFIYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVLRMVINVHEAGRNFTVACQHRSNSEIICCTTPSIEQLNLQLPLKTKAFFMLDGIHSKYFDLIYVHNPVFKPFEKPVMISVGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHSHSEAVLCTVPSDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTGLIVGVVSISIILLLLLGLFLWLKKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNASQNGSCRQVQYPLTDLSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDDKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMEYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDVYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAELRPSFSELVSRISVIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSQDNVSGEDDDDT

Target ORF information:

RefSeq Version	XM_024990407.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990407.1

ORF Insert Sequence:

ATGAAGGCCC CTGCTGTGCT TGCACCTGGC ATTCTTGTGC TTCTGTTTAC CTTCGTGCAG 
AAGAGCAATG GGGAGTGTAA AGAGGCACTA GTAAAATCCA GGATGAATGT GAACATGCAA 
TATCAGCTTC CTAACTTCAC TGCAGAAACA TCCATTCAGA ATGTTGTTTT GCACAAACAT 
CACATTTACC TCGGTGCCAT TAACTACATT TACGTTTTAA ATGACAAAGA CCTTCAGAAG 
GTTGCTGAGT ACAAGACTGG GCCTGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC 
TGCAGCCATA AAGCCAATTT ATCAGGTGGT GTTTGGAAAG ATAACATCAA CATGGCTCTG 
CTTGTTGACA CGTACTATGA TGATCAACTC ATTAGCTGTG GCAGTGTCCA CAGAGGGACC 
TGCCAGCGAC ACGTCCTTCC ACCCAACAAT ACTGCAGACA TAGAGTCGGA GGTTCACTGC 
ATGTACTCTC CTCAGGCAGA CGAAGAGACC AACCAGTGTC CTGACTGTGT GGTGAGTGCC 
CTGGGAACCA AAGTCCTACT GTCTGAAAAG GATCGATTCA TCAACTTCTT CGTGGGCAAT 
ACCATAAATT CTTCTTACCT TCCAGATTAT ATATTGCACT CGATATCAGT GAGAAGGCTA 
AAGGAAACAC AAGATGGTTT TAAGTTTTTG ACAGACCAAT CCTATATTGA TGTTCTACCT 
GAACTCCGAG ATTCCTACCC CATTAAGTAT GTCCACGCCT TTGAAAGCAA CCATTTTATT 
TACTTTTTGA CGGTCCAGCG GGAAACTCTA GATGCTCAGA CTTTTCACAC AAGAATAATC 
AGGTTCTGTT CCGCAGACTC TGGATTGCAT TCGTACATGG AAATGCCTCT GGAGTGTATT 
CTGACGGAAA AGAGAAGAAA GAGATCCACA AAGCAGGAAG TGTTTAATAT TCTCCAAGCT 
GCATATGTCA GTAAGCCTGG GGCTCAGCTC GCTAGGCAAA TAGGTGCCAG CCTGAATGAT 
GACATTCTGT ACGGGGTGTT TGCACAAAGC AAGCCAGATT CCTCAGAACC AATGAATCGT 
TCTGCTGTCT GTGCATTCCC TGTCAAATAT GTCAATGAGT TCTTCAACAA AATCGTCAAC 
AAAAACAATG TGAGATGTCT CCAGCACTTT TATGGCCCCA ACCATGAACA CTGCTTCAAT 
AGGACACTTT TGAGAAATTC ATCAGGATGT GAAGTGCGCA ATGATGAATA TCGAACGGAG 
TTTACCACAG CTTTGCCACG CGTTGACTTA TTCACGGGCC AGTTCAACCA AGTCCTCTTA 
ACATCTATAT CCACCTTTAT CAAAGGAGAT CTCACCATCG CTAATCTCGG GACATCAGAA 
GGTCGATTCA TGCAGGTTGT GGTTTCTCGA TCAGGATCAT TAACTCCTCA TGTGAATTTT 
CACCTGGATT CCCACCCTGT GTCTCCAGAA GTGATTGTGG AGCACCCGCT AAACCAGAAT 
GGCTATACAC TGGTTGTCAC TGGGAAGAAG ATCACCAAGA TCCCGCTGAA TGGCCTGGGC 
TGTGAACATT TCCAGTCCTG CAGTCAGTGC CTCTCAGCCC CTTCCTTTGT GCAATGTGGC 
TGGTGCCATG ATAAATGTGT GCGATTGGAG GAATGCTCCA GTGGAACGTG GACTCAAGAG 
ACCTGTCTGC CAACAATCTA CAAGGTTTTT CCAACTAGTG CCCCCCTTGA AGGAGGGACA 
ACACTGACTG TTTGTGGCTG GGACTTTGGA TTCAAGAGGA ATAATAAATT TGACTTGAAG 
AAAACCAGAG TTCTCCTTGG CAATGAGAGC TGCACCTTAA CCTTAACTGA GAGCACAACA 
AACATGTTGA AATGCACAGT TGGTCCTGCA ATGAATGAAC ATTTCAATAT GTCCATAGTT 
ATTTCAAACA GCCGTGGGTC AGTAGAGTAT AGTGCGTTTT CCTATGTGGA TCCTATAATA 
ACAAGTATTT CTCCAAATTA TGGTCCAAAA ACTGGTGGCA CTTTACTTAC ATTAACTGGA 
AAGCACCTAA ACAGTGGAAA TTCAAGACAC ATTTCAATTG GTGGAAAAAC ATGTACTTTA 
AAAAGTGTTT CACATAGTAT TCTCGAATGT TATACCCCAG CCCAAAGTGC TCCAACTGAG 
TTTTCTGTTA AGTTGAAAAT TGACTTAGCC AACCGAGAGG TGAACAGCTT CATTTACCGG 
GAAGACCCCA TTGTCTATGA AATACATCCC ACCAAATCTT TTATTAGTGG TGGGAGTACA 
ATAACAGGTG TTGGAAAAAA CCTGAATTCA GTTAGTGTCC TAAGGATGGT AATAAATGTG 
CATGAAGCCG GAAGGAACTT TACAGTGGCA TGTCAACATC GCTCTAATTC AGAGATAATC 
TGCTGTACAA CTCCTTCAAT AGAACAGCTG AACCTACAAC TCCCCCTGAA AACCAAAGCC 
TTTTTCATGT TAGATGGGAT CCATTCCAAA TACTTTGATC TCATTTATGT ACATAATCCT 
GTGTTTAAGC CTTTTGAAAA GCCAGTGATG ATCTCAGTAG GCAATGAAAA TGTACTGGAA 
ATTAAGGGAA ATGATATTGA CCCTGAAGCA GTTAAAGGGG AGGTGTTAAA AGTTGGAAAT 
AAGAGCTGTG AAAATATACA CTCACACTCT GAAGCTGTTT TATGTACGGT CCCCAGTGAC 
CTGCTGAAAT TGAACAGCGA ACTAAATATA GAGTGGAAGC AAGCAATTTC TTCAACTGTT 
CTTGGAAAAG TGATAGTTCA ACCAGATCAG AATTTCACAG GATTGATTGT TGGTGTTGTC 
TCAATATCAA TAATACTCTT ACTGTTACTC GGGCTTTTCC TGTGGCTGAA AAAAAGGAAG 
CAAATTAAAG ATCTGGGCAG TGAATTAGTT CGCTATGATG CAAGAGTACA CACTCCACAT 
TTGGATAGGC TTGTCAGTGC CCGAAGTGTA AGCCCAACTA CAGAAATGGT TTCCAATGAA 
TCTGTAGACT ACCGAGCTAC TTTTCCAGAA GACCAGTTTC CTAATGCATC TCAGAATGGG 
TCATGCAGAC AAGTGCAGTA TCCTCTGACA GATCTATCCC CCATCTTAAC TAGTGGGGAC 
TCTGATATAT CCAGTCCATT ACTGCAAAAT ACCGTCCACA TCGACCTCAG TGCTCTAAAT 
CCAGAGCTGG TCCAGGCAGT TCAGCACGTA GTAATTGGGC CGAGCAGTCT GATTGTACAT 
TTCAATGAAG TCATAGGAAG AGGACATTTT GGGTGTGTAT ATCATGGAAC TTTGTTGGAC 
AACGATGATA AGAAAATTCA CTGTGCTGTG AAATCCTTGA ATAGAATCAC TGACATAGGA 
GAAGTTTCCC AATTTCTGAC TGAGGGAATC ATCATGAAAG ATTTTAGTCA TCCCAATGTT 
CTCTCACTTT TGGGAATCTG CCTTCGAAGT GAGGGGTCTC CACTGGTGGT CCTACCATAC 
ATGAAACACG GGGATCTTCG AAATTTCATT AGAAATGAGA CTCATAACCC AACTGTAAAA 
GATCTTATTG GCTTTGGTCT TCAAGTAGCC AAAGGCATGG AATATCTTGC AAGCAAAAAG 
TTTGTCCACA GAGACTTGGC TGCAAGAAAC TGTATGCTGG ATGAAAAATT CACCGTCAAG 
GTTGCTGATT TTGGTCTTGC CAGAGACGTG TATGATAAGG AATACTATAG CGTACACAAC 
AAAACAGGTG CAAAACTGCC AGTGAAATGG ATGGCTTTAG AAAGCCTGCA GACTCAGAAA 
TTTACCACCA AGTCAGATGT GTGGTCCTTT GGCGTGCTTC TCTGGGAACT GATGACAAGA 
GGAGCACCAC CTTATCCTGA CGTCAACACC TTTGACATAA CAGTTTACTT GTTGCAAGGA 
AGAAGGCTCC TACAACCTGA ATACTGCCCA GATCCCTTGT ATGAAGTGAT GCTGAAATGC 
TGGCACCCTA AAGCTGAACT GCGCCCATCC TTTTCTGAAC TGGTCTCCAG GATATCCGTG 
ATATTCTCTA CTTTCATTGG GGAGCACTAT GTCCATGTGA ATGCTACTTA CGTGAACGTC 
AAATGTGTCG CTCCATATCC TTCTCTGTTG TCATCACAAG ATAATGTCAG TGGCGAGGAT 
GATGATGACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MET ( XM_024990408.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846176.1 Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X2, mRNA.

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CloneID	OBa233520
Clone ID Related Accession (Same CDS sequence)	XM_024990408.1
Accession Version	XM_024990408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hepatocyte growth factor receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCCC CTGCTGTGCT TGCACCTGGC ATTCTTGTGC TTCTGTTTAC CTTCGTGCAG 
AAGAGCAATG GGGAGTGTAA AGAGGCACTA GTAAAATCCA GGATGAATGT GAACATGCAA 
TATCAGCTTC CTAACTTCAC TGCAGAAACA TCCATTCAGA ATGTTGTTTT GCACAAACAT 
CACATTTACC TCGGTGCCAT TAACTACATT TACGTTTTAA ATGACAAAGA CCTTCAGAAG 
GTTGCTGAGT ACAAGACTGG GCCTGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC 
TGCAGCCATA AAGCCAATTT ATCAGGTGGT GTTTGGAAAG ATAACATCAA CATGGCTCTG 
CTTGTTGACA CGTACTATGA TGATCAACTC ATTAGCTGTG GCAGTGTCCA CAGAGGGACC 
TGCCAGCGAC ACGTCCTTCC ACCCAACAAT ACTGCAGACA TAGAGTCGGA GGTTCACTGC 
ATGTACTCTC CTCAGGCAGA CGAAGAGACC AACCAGTGTC CTGACTGTGT GGTGAGTGCC 
CTGGGAACCA AAGTCCTACT GTCTGAAAAG GATCGATTCA TCAACTTCTT CGTGGGCAAT 
ACCATAAATT CTTCTTACCT TCCAGATTAT ATATTGCACT CGATATCAGT GAGAAGGCTA 
AAGGAAACAC AAGATGGTTT TAAGTTTTTG ACAGACCAAT CCTATATTGA TGTTCTACCT 
GAACTCCGAG ATTCCTACCC CATTAAGTAT GTCCACGCCT TTGAAAGCAA CCATTTTATT 
TACTTTTTGA CGGTCCAGCG GGAAACTCTA GATGCTCAGA CTTTTCACAC AAGAATAATC 
AGGTTCTGTT CCGCAGACTC TGGATTGCAT TCGTACATGG AAATGCCTCT GGAGTGTATT 
CTGACGGAAA AGAGAAGAAA GAGATCCACA AAGCAGGAAG TGTTTAATAT TCTCCAAGCT 
GCATATGTCA GTAAGCCTGG GGCTCAGCTC GCTAGGCAAA TAGGTGCCAG CCTGAATGAT 
GACATTCTGT ACGGGGTGTT TGCACAAAGC AAGCCAGATT CCTCAGAACC AATGAATCGT 
TCTGCTGTCT GTGCATTCCC TGTCAAATAT GTCAATGAGT TCTTCAACAA AATCGTCAAC 
AAAAACAATG TGAGATGTCT CCAGCACTTT TATGGCCCCA ACCATGAACA CTGCTTCAAT 
AGGACACTTT TGAGAAATTC ATCAGGATGT GAAGTGCGCA ATGATGAATA TCGAACGGAG 
TTTACCACAG CTTTGCCACG CGTTGACTTA TTCACGGGCC AGTTCAACCA AGTCCTCTTA 
ACATCTATAT CCACCTTTAT CAAAGGAGAT CTCACCATCG CTAATCTCGG GACATCAGAA 
GGTCGATTCA TGCAGGTTGT GGTTTCTCGA TCAGGATCAT TAACTCCTCA TGTGAATTTT 
CACCTGGATT CCCACCCTGT GTCTCCAGAA GTGATTGTGG AGCACCCGCT AAACCAGAAT 
GGCTATACAC TGGTTGTCAC TGGGAAGAAG ATCACCAAGA TCCCGCTGAA TGGCCTGGGC 
TGTGAACATT TCCAGTCCTG CAGTCAGTGC CTCTCAGCCC CTTCCTTTGT GCAATGTGGC 
TGGTGCCATG ATAAATGTGT GCGATTGGAG GAATGCTCCA GTGGAACGTG GACTCAAGAG 
ACCTGTCTGC CAACAATCTA CAAGGTTTTT CCAACTAGTG CCCCCCTTGA AGGAGGGACA 
ACACTGACTG TTTGTGGCTG GGACTTTGGA TTCAAGAGGA ATAATAAATT TGACTTGAAG 
AAAACCAGAG TTCTCCTTGG CAATGAGAGC TGCACCTTAA CCTTAACTGA GAGCACAACA 
AACATGTTGA AATGCACAGT TGGTCCTGCA ATGAATGAAC ATTTCAATAT GTCCATAGTT 
ATTTCAAACA GCCGTGGGTC AGTAGAGTAT AGTGCGTTTT CCTATGTGGA TCCTATAATA 
ACAAGTATTT CTCCAAATTA TGGTCCAAAA ACTGGTGGCA CTTTACTTAC ATTAACTGGA 
AAGCACCTAA ACAGTGGAAA TTCAAGACAC ATTTCAATTG GTGGAAAAAC ATGTACTTTA 
AAAAGTGTTT CACATAGTAT TCTCGAATGT TATACCCCAG CCCAAAGTGC TCCAACTGAG 
TTTTCTGTTA AGTTGAAAAT TGACTTAGCC AACCGAGAGG TGAACAGCTT CATTTACCGG 
GAAGACCCCA TTGTCTATGA AATACATCCC ACCAAATCTT TTATTAGTGG TGGGAGTACA 
ATAACAGGTG TTGGAAAAAA CCTGAATTCA GTTAGTGTCC TAAGGATGGT AATAAATGTG 
CATGAAGCCG GAAGGAACTT TACAGTGGCA TGTCAACATC GCTCTAATTC AGAGATAATC 
TGCTGTACAA CTCCTTCAAT AGAACAGCTG AACCTACAAC TCCCCCTGAA AACCAAAGCC 
TTTTTCATGT TAGATGGGAT CCATTCCAAA TACTTTGATC TCATTTATGT ACATAATCCT 
GTGTTTAAGC CTTTTGAAAA GCCAGTGATG ATCTCAGTAG GCAATGAAAA TGTACTGGAA 
ATTAAGGGAA ATGATATTGA CCCTGAAGCA GTTAAAGGGG AGGTGTTAAA AGTTGGAAAT 
AAGAGCTGTG AAAATATACA CTCACACTCT GAAGCTGTTT TATGTACGGT CCCCAGTGAC 
CTGCTGAAAT TGAACAGCGA ACTAAATATA GAGTGGAAGC AAGCAATTTC TTCAACTGTT 
CTTGGAAAAG TGATAGTTCA ACCAGATCAG AATTTCACAG GATTGATTGT TGGTGTTGTC 
TCAATATCAA TAATACTCTT ACTGTTACTC GGGCTTTTCC TGTGGCTGAA AAAAAGGAAG 
CAAATTAAAG ACCAGTTTCC TAATGCATCT CAGAATGGGT CATGCAGACA AGTGCAGTAT 
CCTCTGACAG ATCTATCCCC CATCTTAACT AGTGGGGACT CTGATATATC CAGTCCATTA 
CTGCAAAATA CCGTCCACAT CGACCTCAGT GCTCTAAATC CAGAGCTGGT CCAGGCAGTT 
CAGCACGTAG TAATTGGGCC GAGCAGTCTG ATTGTACATT TCAATGAAGT CATAGGAAGA 
GGACATTTTG GGTGTGTATA TCATGGAACT TTGTTGGACA ACGATGATAA GAAAATTCAC 
TGTGCTGTGA AATCCTTGAA TAGAATCACT GACATAGGAG AAGTTTCCCA ATTTCTGACT 
GAGGGAATCA TCATGAAAGA TTTTAGTCAT CCCAATGTTC TCTCACTTTT GGGAATCTGC 
CTTCGAAGTG AGGGGTCTCC ACTGGTGGTC CTACCATACA TGAAACACGG GGATCTTCGA 
AATTTCATTA GAAATGAGAC TCATAACCCA ACTGTAAAAG ATCTTATTGG CTTTGGTCTT 
CAAGTAGCCA AAGGCATGGA ATATCTTGCA AGCAAAAAGT TTGTCCACAG AGACTTGGCT 
GCAAGAAACT GTATGCTGGA TGAAAAATTC ACCGTCAAGG TTGCTGATTT TGGTCTTGCC 
AGAGACGTGT ATGATAAGGA ATACTATAGC GTACACAACA AAACAGGTGC AAAACTGCCA 
GTGAAATGGA TGGCTTTAGA AAGCCTGCAG ACTCAGAAAT TTACCACCAA GTCAGATGTG 
TGGTCCTTTG GCGTGCTTCT CTGGGAACTG ATGACAAGAG GAGCACCACC TTATCCTGAC 
GTCAACACCT TTGACATAAC AGTTTACTTG TTGCAAGGAA GAAGGCTCCT ACAACCTGAA 
TACTGCCCAG ATCCCTTGTA TGAAGTGATG CTGAAATGCT GGCACCCTAA AGCTGAACTG 
CGCCCATCCT TTTCTGAACT GGTCTCCAGG ATATCCGTGA TATTCTCTAC TTTCATTGGG 
GAGCACTATG TCCATGTGAA TGCTACTTAC GTGAACGTCA AATGTGTCGC TCCATATCCT 
TCTCTGTTGT CATCACAAGA TAATGTCAGT GGCGAGGATG ATGATGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846176.1
CDS	248..4261
Translation	MKAPAVLAPGILVLLFTFVQKSNGECKEALVKSRMNVNMQYQLPNFTAETSIQNVVLHKHHIYLGAINYIYVLNDKDLQKVAEYKTGPVLEHPDCFPCQDCSHKANLSGGVWKDNINMALLVDTYYDDQLISCGSVHRGTCQRHVLPPNNTADIESEVHCMYSPQADEETNQCPDCVVSALGTKVLLSEKDRFINFFVGNTINSSYLPDYILHSISVRRLKETQDGFKFLTDQSYIDVLPELRDSYPIKYVHAFESNHFIYFLTVQRETLDAQTFHTRIIRFCSADSGLHSYMEMPLECILTEKRRKRSTKQEVFNILQAAYVSKPGAQLARQIGASLNDDILYGVFAQSKPDSSEPMNRSAVCAFPVKYVNEFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEVRNDEYRTEFTTALPRVDLFTGQFNQVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGSLTPHVNFHLDSHPVSPEVIVEHPLNQNGYTLVVTGKKITKIPLNGLGCEHFQSCSQCLSAPSFVQCGWCHDKCVRLEECSSGTWTQETCLPTIYKVFPTSAPLEGGTTLTVCGWDFGFKRNNKFDLKKTRVLLGNESCTLTLTESTTNMLKCTVGPAMNEHFNMSIVISNSRGSVEYSAFSYVDPIITSISPNYGPKTGGTLLTLTGKHLNSGNSRHISIGGKTCTLKSVSHSILECYTPAQSAPTEFSVKLKIDLANREVNSFIYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVLRMVINVHEAGRNFTVACQHRSNSEIICCTTPSIEQLNLQLPLKTKAFFMLDGIHSKYFDLIYVHNPVFKPFEKPVMISVGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHSHSEAVLCTVPSDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTGLIVGVVSISIILLLLLGLFLWLKKRKQIKDQFPNASQNGSCRQVQYPLTDLSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDDKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMEYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDVYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAELRPSFSELVSRISVIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSQDNVSGEDDDDT

Target ORF information:

RefSeq Version	XM_024990408.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990408.1

ORF Insert Sequence:

ATGAAGGCCC CTGCTGTGCT TGCACCTGGC ATTCTTGTGC TTCTGTTTAC CTTCGTGCAG 
AAGAGCAATG GGGAGTGTAA AGAGGCACTA GTAAAATCCA GGATGAATGT GAACATGCAA 
TATCAGCTTC CTAACTTCAC TGCAGAAACA TCCATTCAGA ATGTTGTTTT GCACAAACAT 
CACATTTACC TCGGTGCCAT TAACTACATT TACGTTTTAA ATGACAAAGA CCTTCAGAAG 
GTTGCTGAGT ACAAGACTGG GCCTGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC 
TGCAGCCATA AAGCCAATTT ATCAGGTGGT GTTTGGAAAG ATAACATCAA CATGGCTCTG 
CTTGTTGACA CGTACTATGA TGATCAACTC ATTAGCTGTG GCAGTGTCCA CAGAGGGACC 
TGCCAGCGAC ACGTCCTTCC ACCCAACAAT ACTGCAGACA TAGAGTCGGA GGTTCACTGC 
ATGTACTCTC CTCAGGCAGA CGAAGAGACC AACCAGTGTC CTGACTGTGT GGTGAGTGCC 
CTGGGAACCA AAGTCCTACT GTCTGAAAAG GATCGATTCA TCAACTTCTT CGTGGGCAAT 
ACCATAAATT CTTCTTACCT TCCAGATTAT ATATTGCACT CGATATCAGT GAGAAGGCTA 
AAGGAAACAC AAGATGGTTT TAAGTTTTTG ACAGACCAAT CCTATATTGA TGTTCTACCT 
GAACTCCGAG ATTCCTACCC CATTAAGTAT GTCCACGCCT TTGAAAGCAA CCATTTTATT 
TACTTTTTGA CGGTCCAGCG GGAAACTCTA GATGCTCAGA CTTTTCACAC AAGAATAATC 
AGGTTCTGTT CCGCAGACTC TGGATTGCAT TCGTACATGG AAATGCCTCT GGAGTGTATT 
CTGACGGAAA AGAGAAGAAA GAGATCCACA AAGCAGGAAG TGTTTAATAT TCTCCAAGCT 
GCATATGTCA GTAAGCCTGG GGCTCAGCTC GCTAGGCAAA TAGGTGCCAG CCTGAATGAT 
GACATTCTGT ACGGGGTGTT TGCACAAAGC AAGCCAGATT CCTCAGAACC AATGAATCGT 
TCTGCTGTCT GTGCATTCCC TGTCAAATAT GTCAATGAGT TCTTCAACAA AATCGTCAAC 
AAAAACAATG TGAGATGTCT CCAGCACTTT TATGGCCCCA ACCATGAACA CTGCTTCAAT 
AGGACACTTT TGAGAAATTC ATCAGGATGT GAAGTGCGCA ATGATGAATA TCGAACGGAG 
TTTACCACAG CTTTGCCACG CGTTGACTTA TTCACGGGCC AGTTCAACCA AGTCCTCTTA 
ACATCTATAT CCACCTTTAT CAAAGGAGAT CTCACCATCG CTAATCTCGG GACATCAGAA 
GGTCGATTCA TGCAGGTTGT GGTTTCTCGA TCAGGATCAT TAACTCCTCA TGTGAATTTT 
CACCTGGATT CCCACCCTGT GTCTCCAGAA GTGATTGTGG AGCACCCGCT AAACCAGAAT 
GGCTATACAC TGGTTGTCAC TGGGAAGAAG ATCACCAAGA TCCCGCTGAA TGGCCTGGGC 
TGTGAACATT TCCAGTCCTG CAGTCAGTGC CTCTCAGCCC CTTCCTTTGT GCAATGTGGC 
TGGTGCCATG ATAAATGTGT GCGATTGGAG GAATGCTCCA GTGGAACGTG GACTCAAGAG 
ACCTGTCTGC CAACAATCTA CAAGGTTTTT CCAACTAGTG CCCCCCTTGA AGGAGGGACA 
ACACTGACTG TTTGTGGCTG GGACTTTGGA TTCAAGAGGA ATAATAAATT TGACTTGAAG 
AAAACCAGAG TTCTCCTTGG CAATGAGAGC TGCACCTTAA CCTTAACTGA GAGCACAACA 
AACATGTTGA AATGCACAGT TGGTCCTGCA ATGAATGAAC ATTTCAATAT GTCCATAGTT 
ATTTCAAACA GCCGTGGGTC AGTAGAGTAT AGTGCGTTTT CCTATGTGGA TCCTATAATA 
ACAAGTATTT CTCCAAATTA TGGTCCAAAA ACTGGTGGCA CTTTACTTAC ATTAACTGGA 
AAGCACCTAA ACAGTGGAAA TTCAAGACAC ATTTCAATTG GTGGAAAAAC ATGTACTTTA 
AAAAGTGTTT CACATAGTAT TCTCGAATGT TATACCCCAG CCCAAAGTGC TCCAACTGAG 
TTTTCTGTTA AGTTGAAAAT TGACTTAGCC AACCGAGAGG TGAACAGCTT CATTTACCGG 
GAAGACCCCA TTGTCTATGA AATACATCCC ACCAAATCTT TTATTAGTGG TGGGAGTACA 
ATAACAGGTG TTGGAAAAAA CCTGAATTCA GTTAGTGTCC TAAGGATGGT AATAAATGTG 
CATGAAGCCG GAAGGAACTT TACAGTGGCA TGTCAACATC GCTCTAATTC AGAGATAATC 
TGCTGTACAA CTCCTTCAAT AGAACAGCTG AACCTACAAC TCCCCCTGAA AACCAAAGCC 
TTTTTCATGT TAGATGGGAT CCATTCCAAA TACTTTGATC TCATTTATGT ACATAATCCT 
GTGTTTAAGC CTTTTGAAAA GCCAGTGATG ATCTCAGTAG GCAATGAAAA TGTACTGGAA 
ATTAAGGGAA ATGATATTGA CCCTGAAGCA GTTAAAGGGG AGGTGTTAAA AGTTGGAAAT 
AAGAGCTGTG AAAATATACA CTCACACTCT GAAGCTGTTT TATGTACGGT CCCCAGTGAC 
CTGCTGAAAT TGAACAGCGA ACTAAATATA GAGTGGAAGC AAGCAATTTC TTCAACTGTT 
CTTGGAAAAG TGATAGTTCA ACCAGATCAG AATTTCACAG GATTGATTGT TGGTGTTGTC 
TCAATATCAA TAATACTCTT ACTGTTACTC GGGCTTTTCC TGTGGCTGAA AAAAAGGAAG 
CAAATTAAAG ACCAGTTTCC TAATGCATCT CAGAATGGGT CATGCAGACA AGTGCAGTAT 
CCTCTGACAG ATCTATCCCC CATCTTAACT AGTGGGGACT CTGATATATC CAGTCCATTA 
CTGCAAAATA CCGTCCACAT CGACCTCAGT GCTCTAAATC CAGAGCTGGT CCAGGCAGTT 
CAGCACGTAG TAATTGGGCC GAGCAGTCTG ATTGTACATT TCAATGAAGT CATAGGAAGA 
GGACATTTTG GGTGTGTATA TCATGGAACT TTGTTGGACA ACGATGATAA GAAAATTCAC 
TGTGCTGTGA AATCCTTGAA TAGAATCACT GACATAGGAG AAGTTTCCCA ATTTCTGACT 
GAGGGAATCA TCATGAAAGA TTTTAGTCAT CCCAATGTTC TCTCACTTTT GGGAATCTGC 
CTTCGAAGTG AGGGGTCTCC ACTGGTGGTC CTACCATACA TGAAACACGG GGATCTTCGA 
AATTTCATTA GAAATGAGAC TCATAACCCA ACTGTAAAAG ATCTTATTGG CTTTGGTCTT 
CAAGTAGCCA AAGGCATGGA ATATCTTGCA AGCAAAAAGT TTGTCCACAG AGACTTGGCT 
GCAAGAAACT GTATGCTGGA TGAAAAATTC ACCGTCAAGG TTGCTGATTT TGGTCTTGCC 
AGAGACGTGT ATGATAAGGA ATACTATAGC GTACACAACA AAACAGGTGC AAAACTGCCA 
GTGAAATGGA TGGCTTTAGA AAGCCTGCAG ACTCAGAAAT TTACCACCAA GTCAGATGTG 
TGGTCCTTTG GCGTGCTTCT CTGGGAACTG ATGACAAGAG GAGCACCACC TTATCCTGAC 
GTCAACACCT TTGACATAAC AGTTTACTTG TTGCAAGGAA GAAGGCTCCT ACAACCTGAA 
TACTGCCCAG ATCCCTTGTA TGAAGTGATG CTGAAATGCT GGCACCCTAA AGCTGAACTG 
CGCCCATCCT TTTCTGAACT GGTCTCCAGG ATATCCGTGA TATTCTCTAC TTTCATTGGG 
GAGCACTATG TCCATGTGAA TGCTACTTAC GTGAACGTCA AATGTGTCGC TCCATATCCT 
TCTCTGTTGT CATCACAAGA TAATGTCAGT GGCGAGGATG ATGATGACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MET ( NM_001012999.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001013017.2 Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), mRNA.

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CloneID	OBa41548
Clone ID Related Accession (Same CDS sequence)	NM_001012999.2
Accession Version	NM_001012999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	hepatocyte growth factor receptor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB112434.1. On or before Mar 29, 2005 this sequence version replaced XM_608832.1, XM_612507.1, NM_001012999.1. ##Evidence-Data-START## Transcript exon combination :: AB112434.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGCCC CTGCTGTGCT TGCACCTGGC ATTCTTGTGC TTCTGTTTAC CTTCGTGCAG 
AAGAGCAATG GGGAGTGTAA AGAGGCACTA GTAAAATCCA GGATGAATGT GAACATGCAA 
TATCAGCTTC CTAACTTCAC TGCAGAAACA TCCATTCAGA ATGTTGTTTT GCACAAACAT 
CACATTTACC TCGGTGCCAT TAACTACATT TACGTTTTAA ATGACAAAGA CCTTCAGAAG 
GTTGCTGAGT ACAAGACTGG GCCTGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC 
TGCAGCCATA AAGCCAATTT ATCAGGTGGT GTTTGGAAAG ATAACATCAA CATGGCTCTG 
CTTGTTGACA CGTACTATGA TGATCAACTC ATTAGCTGTG GCAGTGTCCA CAGAGGGACC 
TGCCAGCGAC ACGTCCTTCC ACCCAACAAT ACTGCAGACA TAGAGTCGGA GGTTCACTGC 
ATGTACTCTC CTCAGGCAGA CGAAGAGACC AACCAGTGTC CTGACTGTGT GGTGAGTGCC 
CTGGGAACCA AAGTCCTACT GTCTGAAAAG GATCGATTCA TCAACTTCTT CGTGGGCAAT 
ACCATAAATT CTTCTTACCT TCCAGATTAT ATATTGCACT CGATATCAGT GAGAAGGCTA 
AAGGAAACAC AAGATGGTTT TAAGTTTTTG ACAGACCAAT CCTATATTGA TGTTCTACCT 
GAACTCCGAG ATTCCTACCC CATTAAGTAT GTCCACGCCT TTGAAAGCAA CCATTTTATT 
TACTTTTTGA CGGTCCAGCG GGAAACTCTA GATGCTCAGA CTTTTCACAC AAGAATAATC 
AGGTTCTGTT CCGCAGACTC TGGATTGCAT TCGTACATGG AAATGCCTCT GGAGTGTATT 
CTGACGGAAA AGAGAAGAAA GAGATCCACA AAGCAGGAAG TGTTTAATAT TCTCCAAGCT 
GCATATGTCA GTAAGCCTGG GGCTCAGCTC GCTAGGCAAA TAGGTGCCAG CCTGAATGAT 
GACATTCTGT ACGGGGTGTT TGCACAAAGC AAGCCAGATT CCTCAGAACC AATGAATCGT 
TCTGCTGTCT GTGCATTCCC TGTCAAATAT GTCAATGAGT TCTTCAACAA AATCGTCAAC 
AAAAACAATG TGAGATGTCT CCAGCACTTT TATGGCCCCA ACCATGAACA CTGCTTCAAT 
AGGACACTTT TGAGAAATTC ATCAGGATGT GAAGTGCGCA ATGATGAATA TCGAACGGAG 
TTTACCACAG CTTTGCCACG CGTTGACTTA TTCACGGGCC AGTTCAACCA AGTCCTCTTA 
ACATCTATAT CCACCTTTAT CAAAGGAGAT CTCACCATCG CTAATCTCGG GACATCAGAA 
GGTCGATTCA TGCAGGTTGT GGTTTCTCGA TCAGGATCAT TAACTCCTCA TGTGAATTTT 
CACCTGGATT CCCACCCTGT GTCTCCAGAA GTGATTGTGG AGCACCCGCT AAACCAGAAT 
GGCTATACAC TGGTTGTCAC TGGGAAGAAG ATCACCAAGA TCCCGCTGAA TGGCCTGGGC 
TGTGAACATT TCCAGTCCTG CAGTCAGTGC CTCTCAGCCC CTTCCTTTGT GCAATGTGGC 
TGGTGCCATG ATAAATGTGT GCGATTGGAG GAATGCTCCA GTGGAACGTG GACTCAAGAG 
ACCTGTCTGC CAACAATCTA CAAGGTTTTT CCAACTAGTG CCCCCCTTGA AGGAGGGACA 
ACACTGACTG TTTGTGGCTG GGACTTTGGA TTCAAGAGGA ATAATAAATT TGATTTGAAG 
AAAACCAGAG TTCTCCTTGG CAATGAGAGC TGCACCTTAA CCTTAACTGA GAGCACAACA 
AACATGTTGA AATGCACAGT TGGTCCTGCA ATGAATGAAC ATTTCAATAT GTCCATAGTT 
ATTTCAAACA GCCGTGGGTC AGTAGAGTAT AGTGCGTTTT CCTATGTGGA TCCTATAATA 
ACAAGTATTT CTCCAAATTA TGGTCCAAAA ACTGGTGGCA CTTTACTTAC ATTAACTGGA 
AAGCACCTAA ACAGTGGAAA TTCAAGACAC ATTTCAATTG GTGGAAAAAC ATGTACTTTA 
AAAAGTGTTT CACATAGTAT TCTCGAATGT TATACCCCAG CCCAAAGTGC TCCAACTGAG 
TTTTCTGTTA AGTTGAAAAT TGACTTAGCC AACCGAGAGG TGAACAGCTT CATTTACCGG 
GAAGACCCCA TTGTCTATGA AATACATCCC ACCAAATCTT TTATTAGTGG TGGGAGTACA 
ATAACAGGTG TTGGAAAAAA CCTGAATTCA GTTAGTGTCC TAAGGATGGT AATAAATGTG 
CATGAAGCCG GAAGGAACTT TACAGTGGCA TGTCAACATC GCTCTAATTC AGAGATAATC 
TGCTGTACAA CTCCTTCAAT AGAACAGCTG AACCTACAAC TCCCCCTGAA AACCAAAGCC 
TTTTTCATGT TAGATGGGAT CCATTCCAAA TACTTTGATC TCATTTATGT ACATAATCCT 
GTGTTTAAGC CTTTTGAAAA GCCAGTGATG ATCTCAGTAG GCAATGAAAA TGTACTGGAA 
ATTAAGGGAA ATGATATTGA CCCTGAAGCA GTTAAAGGGG AGGTGTTAAA AGTTGGAAAT 
AAGAGCTGTG AAAATATACA CTCACACTCT GAAGCTGTTT TATGTACGGT CCCCAGTGAC 
CTGCTGAAAT TGAACAGCGA ACTAAATATA GAGTGGAAGC AAGCAATTTC TTCAACTGTT 
CTTGGAAAAG TGATAGTTCA ACCAGATCAG AATTTCACAG GATTGATTGT TGGTGTTGTC 
TCAATATCAA TAATACTCTT ACTGTTACTC GGGCTTTTCC TGTGGCTGAA AAAAAGGAAG 
CAAATTAAAG ATCTGGGCAG TGAATTAGTT CGCTATGATG CAAGAGTACA CACTCCACAT 
TTGGATAGGC TTGTCAGTGC CCGAAGTGTA AGCCCAACTA CAGAAATGGT TTCCAATGAA 
TCTGTAGACT ACCGAGCTAC TTTTCCAGAA GACCAGTTTC CTAATGCATC TCAGAATGGG 
TCATGCAGAC AAGTGCAGTA TCCTCTGACA GATCTATCCC CCATCTTAAC TAGTGGGGAC 
TCTGATATAT CCAGTCCATT ACTGCAAAAT ACCATCCACA TCGACCTCAG TGCTCTAAAT 
CCAGAGCTGG TCCAGGCAGT TCAGCACGTA GTAATTGGGC CGAGCAGTCT GATTGTACAT 
TTCAATGAAG TCATAGGAAG AGGACATTTT GGGTGTGTAT ATCATGGAAC TTTGTTGGAC 
AACGATGATA AGAAAATTCA CTGTGCTGTG AAATCCTTGA ATAGAATCAC TGACATAGGA 
GAAGTTTCCC AATTTCTGAC TGAGGGAATC ATCATGAAAG ATTTTAGTCA TCCCAATGTT 
CTCTCACTTT TGGGAATCTG CCTTCGAAGT GAGGGGTCTC CACTGGTGGT CCTACCATAC 
ATGAAACACG GGGATCTTCG AAATTTCATT AGAAATGAGA CTCATAACCC AACTGTAAAA 
GATCTTATTG GCTTTGGTCT TCAAGTAGCC AAAGGCATGG AATATCTTGC AAGCAAAAAG 
TTTGTCCACA GAGACTTGGC TGCAAGAAAC TGTATGCTGG ATGAAAAATT CACCGTCAAG 
GTTGCTGATT TTGGTCTTGC CAGAGACGTG TATGATAAGG AATACTATAG CGTACACAAC 
AAAACAGGTG CAAAACTGCC AGTGAAATGG ATGGCTTTAG AAAGCCTGCA GACTCAGAAA 
TTTACCACCA AGTCAGATGT GTGGTCCTTT GGCGTGCTTC TCTGGGAACT GATGACAAGA 
GGAGCACCAC CTTATCCTGA CGTCAACACC TTTGACATAA CAGTTTACTT GTTGCAAGGA 
AGAAGGCTCC TACAACCTGA ATACTGCCCA GATCCCTTGT ATGAAGTGAT GCTGAAATGC 
TGGCACCCTA AAGCTGAACT GCGCCCATCC TTTTCTGAAC TGGTCTCCAG GATATCCGTG 
ATATTCTCTA CTTTCATTGG GGAGCACTAT GTCCATGTGA ATGCTACTTA CGTGAACGTC 
AAATGTGTCG CTCCATATCC TTCTCTGTTG TCATCACAAG ATAATGTCAG TGGCGAGGAT 
GATGATGACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013017.2
CDS	168..4322
Translation	MKAPAVLAPGILVLLFTFVQKSNGECKEALVKSRMNVNMQYQLPNFTAETSIQNVVLHKHHIYLGAINYIYVLNDKDLQKVAEYKTGPVLEHPDCFPCQDCSHKANLSGGVWKDNINMALLVDTYYDDQLISCGSVHRGTCQRHVLPPNNTADIESEVHCMYSPQADEETNQCPDCVVSALGTKVLLSEKDRFINFFVGNTINSSYLPDYILHSISVRRLKETQDGFKFLTDQSYIDVLPELRDSYPIKYVHAFESNHFIYFLTVQRETLDAQTFHTRIIRFCSADSGLHSYMEMPLECILTEKRRKRSTKQEVFNILQAAYVSKPGAQLARQIGASLNDDILYGVFAQSKPDSSEPMNRSAVCAFPVKYVNEFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEVRNDEYRTEFTTALPRVDLFTGQFNQVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGSLTPHVNFHLDSHPVSPEVIVEHPLNQNGYTLVVTGKKITKIPLNGLGCEHFQSCSQCLSAPSFVQCGWCHDKCVRLEECSSGTWTQETCLPTIYKVFPTSAPLEGGTTLTVCGWDFGFKRNNKFDLKKTRVLLGNESCTLTLTESTTNMLKCTVGPAMNEHFNMSIVISNSRGSVEYSAFSYVDPIITSISPNYGPKTGGTLLTLTGKHLNSGNSRHISIGGKTCTLKSVSHSILECYTPAQSAPTEFSVKLKIDLANREVNSFIYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVLRMVINVHEAGRNFTVACQHRSNSEIICCTTPSIEQLNLQLPLKTKAFFMLDGIHSKYFDLIYVHNPVFKPFEKPVMISVGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHSHSEAVLCTVPSDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTGLIVGVVSISIILLLLLGLFLWLKKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNASQNGSCRQVQYPLTDLSPILTSGDSDISSPLLQNTIHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDDKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMEYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDVYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAELRPSFSELVSRISVIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSQDNVSGEDDDDT

Target ORF information:

RefSeq Version	NM_001012999.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MET proto-oncogene, receptor tyrosine kinase (MET), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012999.2

ORF Insert Sequence:

ATGAAGGCCC CTGCTGTGCT TGCACCTGGC ATTCTTGTGC TTCTGTTTAC CTTCGTGCAG 
AAGAGCAATG GGGAGTGTAA AGAGGCACTA GTAAAATCCA GGATGAATGT GAACATGCAA 
TATCAGCTTC CTAACTTCAC TGCAGAAACA TCCATTCAGA ATGTTGTTTT GCACAAACAT 
CACATTTACC TCGGTGCCAT TAACTACATT TACGTTTTAA ATGACAAAGA CCTTCAGAAG 
GTTGCTGAGT ACAAGACTGG GCCTGTGCTG GAACACCCAG ATTGTTTCCC ATGTCAGGAC 
TGCAGCCATA AAGCCAATTT ATCAGGTGGT GTTTGGAAAG ATAACATCAA CATGGCTCTG 
CTTGTTGACA CGTACTATGA TGATCAACTC ATTAGCTGTG GCAGTGTCCA CAGAGGGACC 
TGCCAGCGAC ACGTCCTTCC ACCCAACAAT ACTGCAGACA TAGAGTCGGA GGTTCACTGC 
ATGTACTCTC CTCAGGCAGA CGAAGAGACC AACCAGTGTC CTGACTGTGT GGTGAGTGCC 
CTGGGAACCA AAGTCCTACT GTCTGAAAAG GATCGATTCA TCAACTTCTT CGTGGGCAAT 
ACCATAAATT CTTCTTACCT TCCAGATTAT ATATTGCACT CGATATCAGT GAGAAGGCTA 
AAGGAAACAC AAGATGGTTT TAAGTTTTTG ACAGACCAAT CCTATATTGA TGTTCTACCT 
GAACTCCGAG ATTCCTACCC CATTAAGTAT GTCCACGCCT TTGAAAGCAA CCATTTTATT 
TACTTTTTGA CGGTCCAGCG GGAAACTCTA GATGCTCAGA CTTTTCACAC AAGAATAATC 
AGGTTCTGTT CCGCAGACTC TGGATTGCAT TCGTACATGG AAATGCCTCT GGAGTGTATT 
CTGACGGAAA AGAGAAGAAA GAGATCCACA AAGCAGGAAG TGTTTAATAT TCTCCAAGCT 
GCATATGTCA GTAAGCCTGG GGCTCAGCTC GCTAGGCAAA TAGGTGCCAG CCTGAATGAT 
GACATTCTGT ACGGGGTGTT TGCACAAAGC AAGCCAGATT CCTCAGAACC AATGAATCGT 
TCTGCTGTCT GTGCATTCCC TGTCAAATAT GTCAATGAGT TCTTCAACAA AATCGTCAAC 
AAAAACAATG TGAGATGTCT CCAGCACTTT TATGGCCCCA ACCATGAACA CTGCTTCAAT 
AGGACACTTT TGAGAAATTC ATCAGGATGT GAAGTGCGCA ATGATGAATA TCGAACGGAG 
TTTACCACAG CTTTGCCACG CGTTGACTTA TTCACGGGCC AGTTCAACCA AGTCCTCTTA 
ACATCTATAT CCACCTTTAT CAAAGGAGAT CTCACCATCG CTAATCTCGG GACATCAGAA 
GGTCGATTCA TGCAGGTTGT GGTTTCTCGA TCAGGATCAT TAACTCCTCA TGTGAATTTT 
CACCTGGATT CCCACCCTGT GTCTCCAGAA GTGATTGTGG AGCACCCGCT AAACCAGAAT 
GGCTATACAC TGGTTGTCAC TGGGAAGAAG ATCACCAAGA TCCCGCTGAA TGGCCTGGGC 
TGTGAACATT TCCAGTCCTG CAGTCAGTGC CTCTCAGCCC CTTCCTTTGT GCAATGTGGC 
TGGTGCCATG ATAAATGTGT GCGATTGGAG GAATGCTCCA GTGGAACGTG GACTCAAGAG 
ACCTGTCTGC CAACAATCTA CAAGGTTTTT CCAACTAGTG CCCCCCTTGA AGGAGGGACA 
ACACTGACTG TTTGTGGCTG GGACTTTGGA TTCAAGAGGA ATAATAAATT TGATTTGAAG 
AAAACCAGAG TTCTCCTTGG CAATGAGAGC TGCACCTTAA CCTTAACTGA GAGCACAACA 
AACATGTTGA AATGCACAGT TGGTCCTGCA ATGAATGAAC ATTTCAATAT GTCCATAGTT 
ATTTCAAACA GCCGTGGGTC AGTAGAGTAT AGTGCGTTTT CCTATGTGGA TCCTATAATA 
ACAAGTATTT CTCCAAATTA TGGTCCAAAA ACTGGTGGCA CTTTACTTAC ATTAACTGGA 
AAGCACCTAA ACAGTGGAAA TTCAAGACAC ATTTCAATTG GTGGAAAAAC ATGTACTTTA 
AAAAGTGTTT CACATAGTAT TCTCGAATGT TATACCCCAG CCCAAAGTGC TCCAACTGAG 
TTTTCTGTTA AGTTGAAAAT TGACTTAGCC AACCGAGAGG TGAACAGCTT CATTTACCGG 
GAAGACCCCA TTGTCTATGA AATACATCCC ACCAAATCTT TTATTAGTGG TGGGAGTACA 
ATAACAGGTG TTGGAAAAAA CCTGAATTCA GTTAGTGTCC TAAGGATGGT AATAAATGTG 
CATGAAGCCG GAAGGAACTT TACAGTGGCA TGTCAACATC GCTCTAATTC AGAGATAATC 
TGCTGTACAA CTCCTTCAAT AGAACAGCTG AACCTACAAC TCCCCCTGAA AACCAAAGCC 
TTTTTCATGT TAGATGGGAT CCATTCCAAA TACTTTGATC TCATTTATGT ACATAATCCT 
GTGTTTAAGC CTTTTGAAAA GCCAGTGATG ATCTCAGTAG GCAATGAAAA TGTACTGGAA 
ATTAAGGGAA ATGATATTGA CCCTGAAGCA GTTAAAGGGG AGGTGTTAAA AGTTGGAAAT 
AAGAGCTGTG AAAATATACA CTCACACTCT GAAGCTGTTT TATGTACGGT CCCCAGTGAC 
CTGCTGAAAT TGAACAGCGA ACTAAATATA GAGTGGAAGC AAGCAATTTC TTCAACTGTT 
CTTGGAAAAG TGATAGTTCA ACCAGATCAG AATTTCACAG GATTGATTGT TGGTGTTGTC 
TCAATATCAA TAATACTCTT ACTGTTACTC GGGCTTTTCC TGTGGCTGAA AAAAAGGAAG 
CAAATTAAAG ATCTGGGCAG TGAATTAGTT CGCTATGATG CAAGAGTACA CACTCCACAT 
TTGGATAGGC TTGTCAGTGC CCGAAGTGTA AGCCCAACTA CAGAAATGGT TTCCAATGAA 
TCTGTAGACT ACCGAGCTAC TTTTCCAGAA GACCAGTTTC CTAATGCATC TCAGAATGGG 
TCATGCAGAC AAGTGCAGTA TCCTCTGACA GATCTATCCC CCATCTTAAC TAGTGGGGAC 
TCTGATATAT CCAGTCCATT ACTGCAAAAT ACCATCCACA TCGACCTCAG TGCTCTAAAT 
CCAGAGCTGG TCCAGGCAGT TCAGCACGTA GTAATTGGGC CGAGCAGTCT GATTGTACAT 
TTCAATGAAG TCATAGGAAG AGGACATTTT GGGTGTGTAT ATCATGGAAC TTTGTTGGAC 
AACGATGATA AGAAAATTCA CTGTGCTGTG AAATCCTTGA ATAGAATCAC TGACATAGGA 
GAAGTTTCCC AATTTCTGAC TGAGGGAATC ATCATGAAAG ATTTTAGTCA TCCCAATGTT 
CTCTCACTTT TGGGAATCTG CCTTCGAAGT GAGGGGTCTC CACTGGTGGT CCTACCATAC 
ATGAAACACG GGGATCTTCG AAATTTCATT AGAAATGAGA CTCATAACCC AACTGTAAAA 
GATCTTATTG GCTTTGGTCT TCAAGTAGCC AAAGGCATGG AATATCTTGC AAGCAAAAAG 
TTTGTCCACA GAGACTTGGC TGCAAGAAAC TGTATGCTGG ATGAAAAATT CACCGTCAAG 
GTTGCTGATT TTGGTCTTGC CAGAGACGTG TATGATAAGG AATACTATAG CGTACACAAC 
AAAACAGGTG CAAAACTGCC AGTGAAATGG ATGGCTTTAG AAAGCCTGCA GACTCAGAAA 
TTTACCACCA AGTCAGATGT GTGGTCCTTT GGCGTGCTTC TCTGGGAACT GATGACAAGA 
GGAGCACCAC CTTATCCTGA CGTCAACACC TTTGACATAA CAGTTTACTT GTTGCAAGGA 
AGAAGGCTCC TACAACCTGA ATACTGCCCA GATCCCTTGT ATGAAGTGAT GCTGAAATGC 
TGGCACCCTA AAGCTGAACT GCGCCCATCC TTTTCTGAAC TGGTCTCCAG GATATCCGTG 
ATATTCTCTA CTTTCATTGG GGAGCACTAT GTCCATGTGA ATGCTACTTA CGTGAACGTC 
AAATGTGTCG CTCCATATCC TTCTCTGTTG TCATCACAAG ATAATGTCAG TGGCGAGGAT 
GATGATGACA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epidermal growth factor and hepatocyte growth factor receptors collaborate to induce multiple biological responses in bovine mammary epithelial cells.
Journal of dairy science92(8)3667-75(2009 Aug)
Accornero P,Martignani E,Miretti S,Starvaggi Cucuzza L,Baratta M

Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland.
Domestic animal endocrinology30(3)239-46(2006 Mar)
Yamaji D,Kimura K,Watanabe A,Kon Y,Iwanaga T,Soliman MM,Ahmed MM,Saito M

Comparative analyses of multi-species sequences from targeted genomic regions.
Nature424(6950)788-93(2003 Aug)
Thomas JW,Touchman JW,Blakesley RW,Bouffard GG,Beckstrom-Sternberg SM,Margulies EH,Blanchette M,Siepel AC,Thomas PJ,McDowell JC,Maskeri B,Hansen NF,Schwartz MS,Weber RJ,Kent WJ,Karolchik D,Bruen TC,Bevan R,Cutler DJ,Schwartz S,Elnitski L,Idol JR,Prasad AB,Lee-Lin SQ,Maduro VV,Summers TJ,Portnoy ME,Dietrich NL,Akhter N,Ayele K,Benjamin B,Cariaga K,Brinkley CP,Brooks SY,Granite S,Guan X,Gupta J,Haghighi P,Ho SL,Huang MC,Karlins E,Laric PL,Legaspi R,Lim MJ,Maduro QL,Masiello CA,Mastrian SD,McCloskey JC,Pearson R,Stantripop S,Tiongson EE,Tran JT,Tsurgeon C,Vogt JL,Walker MA,Wetherby KD,Wiggins LS,Young AC,Zhang LH,Osoegawa K,Zhu B,Zhao B,Shu CL,De Jong PJ,Lawrence CE,Smit AF,Chakravarti A,Haussler D,Green P,Miller W,Green ED

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MET cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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