HK1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HK1
Entrez Gene ID	280817
Full Name	hexokinase 1
General protein information	Preferred Names hexokinase 1 Names hexokinase-1 HK I brain form hexokinase hexokinase I hexokinase type I
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012668.1	NP_001012686.1	hexokinase-1
NM_001012668.2	NP_001012686.1	hexokinase-1
XM_010820525.2	XP_010818827.1	hexokinase-1 isoform X2
XM_010820525.1	XP_010818827.1	hexokinase-1 isoform X1
XM_024986501.1	XP_024842269.1	hexokinase-1 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hex-A	NP_524848.1
Saccharomyces cerevisiae (baker's yeast)	HXK2	NP_011261.1
Oryza sativa (rice)	Os05g0522500	NP_001056082.1
Xenopus tropicalis (tropical clawed frog)	hk1	NP_001096201.1
Homo sapiens (human)	HK1	NP_000179.2
Bos taurus (cattle)	HK1	NP_001012686.1
Magnaporthe oryzae (rice blast fungus)	MGG_09289	XP_003709938.1
	HK1	XP_001110396.2
Mus musculus (house mouse)	Hk1	NP_001139572.1
Gallus gallus (chicken)	HK1	NP_989432.1
Eremothecium gossypii	AGOS_AFR279C	NP_985826.1
Canis lupus familiaris (dog)	HK1	XP_536376.3
Rattus norvegicus (Norway rat)	Hk1	NP_036866.1
Kluyveromyces lactis	KLLA0D11352g	XP_453567.1
Arabidopsis thaliana (thale cress)	HXK2	NP_179576.1
Oryza sativa (rice)	Os01g0742500	NP_001044214.1
Pan troglodytes (chimpanzee)	HK1	XP_001169264.2
Danio rerio (zebrafish)	hk1	NP_998417.1
Drosophila melanogaster (fruit fly)	Hex-t2	NP_733151.2
Drosophila melanogaster (fruit fly)	Hex-C	NP_524674.1
Saccharomyces cerevisiae (baker's yeast)	HXK1	NP_116711.3
Schizosaccharomyces pombe (fission yeast)	hxk1	NP_592948.1
Neurospora crassa	NCU02542	XP_965673.2
Arabidopsis thaliana (thale cress)	HXK1	NP_194642.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Bovine hexokinase type I: full-length cDNA sequence and characterisation of the recombinant enzyme.
Andreoni F, Serafini G, Laguardia ME, Magnani M
Molecular and cellular biochemistry268(1-2)9-18(2005 Jan)

Mammalian hexokinase 1: evolutionary conservation and structure to function analysis.
Griffin LD, Gelb BD, Wheeler DA, Davison D, Adams V, McCabe ER
Genomics11(4)1014-24(1991 Dec)

Synthesis and characterization of a bovine hexokinase 1 cDNA probe by mixed oligonucleotide primed amplification of cDNA using high complexity primer mixtures.
Griffin LD, MacGregor GR, Muzny DM, Harter J, Cook RG, McCabe ER
Biochemical medicine and metabolic biology41(2)125-31(1989 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa45017	XM_010820525.2 Latest version!	Bos taurus hexokinase 1 (HK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa239207	NM_001012668.2 Latest version!	Bos taurus hexokinase 1 (HK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa41322	NM_001012668.1 Latest version!	Bos taurus hexokinase 1 (HK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa45016	XM_024986501.1 Latest version!	Bos taurus hexokinase 1 (HK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa45017	XM_010820525.1	Bos taurus hexokinase 1 (HK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HK1 ( XM_010820525.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818827.1 Bos taurus hexokinase 1 (HK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45017
Clone ID Related Accession (Same CDS sequence)	XM_010820525.2 , XM_010820525.1
Accession Version	XM_010820525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hexokinase-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTG ACCACAGTCC GAGTTTGCCC TGTGGAAGTG CTGAGGCCTG GGAAACTGCA 
ATTGACAAGT ATCTCTATGC CATGCGGCTC TCTGATGAAA CTCTGTTAGA TATCATGAAT 
CGTTTCAAAA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCGAC AGCCACAGTC 
AAGATGTTGC CAACGTTTGT AAGGTCTATT CCCGATGGCT CAGAAAAGGG AGATTTCATC 
GCCCTGGATC TTGGCGGATC TTCCTTTCGA ATTCTGCGGG TGCAGGTGAA TCACGAGCAG 
AACCAGGCTG TTCATATGGA GTCTGAGGTT TACGACACCC CGGAGAACAT CATGCATGGC 
AGTGGAAGCC AGCTTTTTGA TCACGTCGCT GAGTGCCTGG GAGACTTCAT GGAGAAAAAA 
AAAATCAAGG ACAAGAAATT ACCTGTGGGA TTCACGTTTT CTTTCCCTTG TCGGCAATCC 
AGAATAGACG AGGCCATCCT GATCACCTGG ACAAAGAGAT TTAAAGCGAG CGGCGTGGAG 
GGAACTGATG TGGTGAAGCT GCTTGACAAA GCCATCAAAA AGCGTGGGGA CTATGATGCC 
AACATTGTGG CTGTGGTGAA CGACACGGTG GGGACCATGA TGACCTGTGG CTACGACGAC 
CAGCAGTGTG AAGTCGGCCT CATCATTGGT ACCGGCACCA ACGCGTGCTA CATGGAGGAG 
CTGAGACACA TCGACCTGGT GGAGGGCGAC GAGGGGAGGA TGTGCGTCAA CACCGAGTGG 
GGGGCCTTTG GGGACGACGG GGCCCTGGAG GACATCCGCA CCGAGTTCGA CCGGGAGATA 
GACCGGGGGT CGCTCAACCC CGGGAAGCAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC 
CTGGGCGAGC TGGTCCGACT GATCCTGGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA 
GGGCGGATCA CCCCAGAGCT GCTCACCAGA GGGAAGTTTA ACACCAGTGA CGTGTCGGCC 
ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG 
GAGCCGTCTG ATGACGACTG TGTTGCGGTT CAGCATGTGT GCACCATCGT CTCATTTCGC 
TCTGCCAACC TGGTGGCGGC GACCCTGGGT GCCATCTTGA GCCGCCTTCG AGATAACAAG 
GGCACGCCCA GGCTGCGGAC CACGGTTGGT GTCGACGGGT CCCTTTACAA GACGCACCCA 
CAGTATTCTC GGCGTTTCCA CAAGACTCTG CGGCGCTTGG TACCCGACTG TGATGTGCGT 
TTCCTCCTCT CCGAGAGCGG CAGTGGCAAG GGGGCCGCCA TGGTGACTGC CGTGGCCTAC 
CGCCTGGCTG AGCAGCACCG GCAGATAGAG GAGACCCTGG CCCACTTCAG CCTCACCAAG 
GAAATGCTGC TGGAGGTGAA AAAGCGGATG CGGGCTGAGA TGGAATTGGG GCTGGGCAAG 
CAGACGCATG ACAAGGCTGT GGTCAAGATG TTGCCCTCCT TCGTCCGGAG CACTCCTGAT 
GGGACTGAGA ACGGTGACTT CTTGGCCCTG GATCTTGGAG GAACGAATTT CCGCGTCCTG 
CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA TCGGTGGAAA TGCACAATAA GATCTACGCC 
ATTCCCATTG AAATCATGCA GGGCACTGGG GAGGAGCTGT TTGACCACAT CGTTTCCTGC 
ATCTCTGACT TCCTGGACTA CATGGGCATC AAGGGCCCTA AAATGCCTCT GGGCTTCACA 
TTTTCGTTTC CCTGCAAGCA GACGAGCTTA GATGCGGGTA TCTTAATCAC CTGGACCAAA 
GGTTTTAAGG CAACCGACTG CGTGGGGCAT GATGTAGCAA CCTTACTGAG GGAGGCGATA 
AAAAGGAGAG AGGAATTTGA CCTGGATGTG GTGGCTGTGG TCAACGACAC GGTGGGCACC 
ATGATGACCT GTGCTTATGA GGAGCCCACC TGCGAGGTTG GACTCATCGT AGGGACTGGC 
AGCAATGCCT GCTACATGGA GGAAATGAAA AACGTCGAGA CGCTAGAAGG AAACCAGGGA 
CAAATGTGTA TCAACATGGA GTGGGGTGCC TTTGGGGACA ACGGGTGTCT GGATGATATC 
AGAACAATCT ATGACAAACT GGTGGATGAG TTTTCTCTAA ACTCTGGGAA GCAAAGGTAT 
GAGAAGATGA TCAGCGGTAT GTACCTGGGT GAGATTGTCC GCAACATCTT GATCGACTTC 
GCCAAGAGGG GCTTCCTCTT CCGGGGGCAG ATCTCTGAGC CGCTGAAGAC CCGGGGCCTC 
TTTCAGACCA AGTATCTCTC TCAGATCGAG AGCGACCGAT TAGCGCTGCT GCAGGTCCGG 
GCCATCCTCC AGCAGCTGGG CCTGAACAGC ACGTGCGACG ACAGTATCCT CGTGAAGACC 
GTGTGTGGGG TGGTGTCCAA GAGGGCGGCG CAGCTGTGTG GCGCAGGCAT GGCTGCTGTG 
GTGGATAAGA TCCGCGAAAA CAGGGGGCTG GACCGCCTCA ACGTGACTGT GGGCGTGGAC 
GGGACGCTCT ACAAGCTTCA TCCGCACTTC TCCAGAATCA TGCACCAAAC CGTGAAGGAA 
CTGTCACCAA AGTGTAATGT GTCCTTCCTC TTATCTGAAG ACGGCAGCGG CAAGGGAGCC 
GCCCTCATCA CAGCGGTGGG CGTTCGGCTC CGGCAAGAAA TGAGCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818827.1
CDS	109..2859
Translation	MDSDHSPSLPCGSAEAWETAIDKYLYAMRLSDETLLDIMNRFKKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEQNQAVHMESEVYDTPENIMHGSGSQLFDHVAECLGDFMEKKKIKDKKLPVGFTFSFPCRQSRIDEAILITWTKRFKASGVEGTDVVKLLDKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCVNTEWGAFGDDGALEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVAVQHVCTIVSFRSANLVAATLGAILSRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDCDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFSLTKEMLLEVKKRMRAEMELGLGKQTHDKAVVKMLPSFVRSTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRSVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPKMPLGFTFSFPCKQTSLDAGILITWTKGFKATDCVGHDVATLLREAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVETLEGNQGQMCINMEWGAFGDNGCLDDIRTIYDKLVDEFSLNSGKQRYEKMISGMYLGEIVRNILIDFAKRGFLFRGQISEPLKTRGLFQTKYLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRQEMSS

Target ORF information:

RefSeq Version	XM_010820525.2
Organism	Bos taurus(cattle)
Definition	Bos taurus hexokinase 1 (HK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820525.2

ORF Insert Sequence:

ATGGACTCTG ACCACAGTCC GAGTTTGCCC TGTGGAAGTG CTGAGGCCTG GGAAACTGCA 
ATTGACAAGT ATCTCTATGC CATGCGGCTC TCTGATGAAA CTCTGTTAGA TATCATGAAT 
CGTTTCAAAA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCGAC AGCCACAGTC 
AAGATGTTGC CAACGTTTGT AAGGTCTATT CCCGATGGCT CAGAAAAGGG AGATTTCATC 
GCCCTGGATC TTGGCGGATC TTCCTTTCGA ATTCTGCGGG TGCAGGTGAA TCACGAGCAG 
AACCAGGCTG TTCATATGGA GTCTGAGGTT TACGACACCC CGGAGAACAT CATGCATGGC 
AGTGGAAGCC AGCTTTTTGA TCACGTCGCT GAGTGCCTGG GAGACTTCAT GGAGAAAAAA 
AAAATCAAGG ACAAGAAATT ACCTGTGGGA TTCACGTTTT CTTTCCCTTG TCGGCAATCC 
AGAATAGACG AGGCCATCCT GATCACCTGG ACAAAGAGAT TTAAAGCGAG CGGCGTGGAG 
GGAACTGATG TGGTGAAGCT GCTTGACAAA GCCATCAAAA AGCGTGGGGA CTATGATGCC 
AACATTGTGG CTGTGGTGAA CGACACGGTG GGGACCATGA TGACCTGTGG CTACGACGAC 
CAGCAGTGTG AAGTCGGCCT CATCATTGGT ACCGGCACCA ACGCGTGCTA CATGGAGGAG 
CTGAGACACA TCGACCTGGT GGAGGGCGAC GAGGGGAGGA TGTGCGTCAA CACCGAGTGG 
GGGGCCTTTG GGGACGACGG GGCCCTGGAG GACATCCGCA CCGAGTTCGA CCGGGAGATA 
GACCGGGGGT CGCTCAACCC CGGGAAGCAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC 
CTGGGCGAGC TGGTCCGACT GATCCTGGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA 
GGGCGGATCA CCCCAGAGCT GCTCACCAGA GGGAAGTTTA ACACCAGTGA CGTGTCGGCC 
ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG 
GAGCCGTCTG ATGACGACTG TGTTGCGGTT CAGCATGTGT GCACCATCGT CTCATTTCGC 
TCTGCCAACC TGGTGGCGGC GACCCTGGGT GCCATCTTGA GCCGCCTTCG AGATAACAAG 
GGCACGCCCA GGCTGCGGAC CACGGTTGGT GTCGACGGGT CCCTTTACAA GACGCACCCA 
CAGTATTCTC GGCGTTTCCA CAAGACTCTG CGGCGCTTGG TACCCGACTG TGATGTGCGT 
TTCCTCCTCT CCGAGAGCGG CAGTGGCAAG GGGGCCGCCA TGGTGACTGC CGTGGCCTAC 
CGCCTGGCTG AGCAGCACCG GCAGATAGAG GAGACCCTGG CCCACTTCAG CCTCACCAAG 
GAAATGCTGC TGGAGGTGAA AAAGCGGATG CGGGCTGAGA TGGAATTGGG GCTGGGCAAG 
CAGACGCATG ACAAGGCTGT GGTCAAGATG TTGCCCTCCT TCGTCCGGAG CACTCCTGAT 
GGGACTGAGA ACGGTGACTT CTTGGCCCTG GATCTTGGAG GAACGAATTT CCGCGTCCTG 
CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA TCGGTGGAAA TGCACAATAA GATCTACGCC 
ATTCCCATTG AAATCATGCA GGGCACTGGG GAGGAGCTGT TTGACCACAT CGTTTCCTGC 
ATCTCTGACT TCCTGGACTA CATGGGCATC AAGGGCCCTA AAATGCCTCT GGGCTTCACA 
TTTTCGTTTC CCTGCAAGCA GACGAGCTTA GATGCGGGTA TCTTAATCAC CTGGACCAAA 
GGTTTTAAGG CAACCGACTG CGTGGGGCAT GATGTAGCAA CCTTACTGAG GGAGGCGATA 
AAAAGGAGAG AGGAATTTGA CCTGGATGTG GTGGCTGTGG TCAACGACAC GGTGGGCACC 
ATGATGACCT GTGCTTATGA GGAGCCCACC TGCGAGGTTG GACTCATCGT AGGGACTGGC 
AGCAATGCCT GCTACATGGA GGAAATGAAA AACGTCGAGA CGCTAGAAGG AAACCAGGGA 
CAAATGTGTA TCAACATGGA GTGGGGTGCC TTTGGGGACA ACGGGTGTCT GGATGATATC 
AGAACAATCT ATGACAAACT GGTGGATGAG TTTTCTCTAA ACTCTGGGAA GCAAAGGTAT 
GAGAAGATGA TCAGCGGTAT GTACCTGGGT GAGATTGTCC GCAACATCTT GATCGACTTC 
GCCAAGAGGG GCTTCCTCTT CCGGGGGCAG ATCTCTGAGC CGCTGAAGAC CCGGGGCCTC 
TTTCAGACCA AGTATCTCTC TCAGATCGAG AGCGACCGAT TAGCGCTGCT GCAGGTCCGG 
GCCATCCTCC AGCAGCTGGG CCTGAACAGC ACGTGCGACG ACAGTATCCT CGTGAAGACC 
GTGTGTGGGG TGGTGTCCAA GAGGGCGGCG CAGCTGTGTG GCGCAGGCAT GGCTGCTGTG 
GTGGATAAGA TCCGCGAAAA CAGGGGGCTG GACCGCCTCA ACGTGACTGT GGGCGTGGAC 
GGGACGCTCT ACAAGCTTCA TCCGCACTTC TCCAGAATCA TGCACCAAAC CGTGAAGGAA 
CTGTCACCAA AGTGTAATGT GTCCTTCCTC TTATCTGAAG ACGGCAGCGG CAAGGGAGCC 
GCCCTCATCA CAGCGGTGGG CGTTCGGCTC CGGCAAGAAA TGAGCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK1 ( XM_010820525.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818827.1 Bos taurus hexokinase 1 (HK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45017
Clone ID Related Accession (Same CDS sequence)	XM_010820525.2 , XM_010820525.1
Accession Version	XM_010820525.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	hexokinase-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTG ACCACAGTCC GAGTTTGCCC TGTGGAAGTG CTGAGGCCTG GGAAACTGCA 
ATTGACAAGT ATCTCTATGC CATGCGGCTC TCTGATGAAA CTCTGTTAGA TATCATGAAT 
CGTTTCAAAA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCGAC AGCCACAGTC 
AAGATGTTGC CAACGTTTGT AAGGTCTATT CCCGATGGCT CAGAAAAGGG AGATTTCATC 
GCCCTGGATC TTGGCGGATC TTCCTTTCGA ATTCTGCGGG TGCAGGTGAA TCACGAGCAG 
AACCAGGCTG TTCATATGGA GTCTGAGGTT TACGACACCC CGGAGAACAT CATGCATGGC 
AGTGGAAGCC AGCTTTTTGA TCACGTCGCT GAGTGCCTGG GAGACTTCAT GGAGAAAAAA 
AAAATCAAGG ACAAGAAATT ACCTGTGGGA TTCACGTTTT CTTTCCCTTG TCGGCAATCC 
AGAATAGACG AGGCCATCCT GATCACCTGG ACAAAGAGAT TTAAAGCGAG CGGCGTGGAG 
GGAACTGATG TGGTGAAGCT GCTTGACAAA GCCATCAAAA AGCGTGGGGA CTATGATGCC 
AACATTGTGG CTGTGGTGAA CGACACGGTG GGGACCATGA TGACCTGTGG CTACGACGAC 
CAGCAGTGTG AAGTCGGCCT CATCATTGGT ACCGGCACCA ACGCGTGCTA CATGGAGGAG 
CTGAGACACA TCGACCTGGT GGAGGGCGAC GAGGGGAGGA TGTGCGTCAA CACCGAGTGG 
GGGGCCTTTG GGGACGACGG GGCCCTGGAG GACATCCGCA CCGAGTTCGA CCGGGAGATA 
GACCGGGGGT CGCTCAACCC CGGGAAGCAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC 
CTGGGCGAGC TGGTCCGACT GATCCTGGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA 
GGGCGGATCA CCCCAGAGCT GCTCACCAGA GGGAAGTTTA ACACCAGTGA CGTGTCGGCC 
ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG 
GAGCCGTCTG ATGACGACTG TGTTGCGGTT CAGCATGTGT GCACCATCGT CTCATTTCGC 
TCTGCCAACC TGGTGGCGGC GACCCTGGGT GCCATCTTGA GCCGCCTTCG AGATAACAAG 
GGCACGCCCA GGCTGCGGAC CACGGTTGGT GTCGACGGGT CCCTTTACAA GACGCACCCA 
CAGTATTCTC GGCGTTTCCA CAAGACTCTG CGGCGCTTGG TACCCGACTG TGATGTGCGT 
TTCCTCCTCT CCGAGAGCGG CAGTGGCAAG GGGGCCGCCA TGGTGACTGC CGTGGCCTAC 
CGCCTGGCTG AGCAGCACCG GCAGATAGAG GAGACCCTGG CCCACTTCAG CCTCACCAAG 
GAAATGCTGC TGGAGGTGAA AAAGCGGATG CGGGCTGAGA TGGAATTGGG GCTGGGCAAG 
CAGACGCATG ACAAGGCTGT GGTCAAGATG TTGCCCTCCT TCGTCCGGAG CACTCCTGAT 
GGGACTGAGA ACGGTGACTT CTTGGCCCTG GATCTTGGAG GAACGAATTT CCGCGTCCTG 
CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA TCGGTGGAAA TGCACAATAA GATCTACGCC 
ATTCCCATTG AAATCATGCA GGGCACTGGG GAGGAGCTGT TTGACCACAT CGTTTCCTGC 
ATCTCTGACT TCCTGGACTA CATGGGCATC AAGGGCCCTA AAATGCCTCT GGGCTTCACA 
TTTTCGTTTC CCTGCAAGCA GACGAGCTTA GATGCGGGTA TCTTAATCAC CTGGACCAAA 
GGTTTTAAGG CAACCGACTG CGTGGGGCAT GATGTAGCAA CCTTACTGAG GGAGGCGATA 
AAAAGGAGAG AGGAATTTGA CCTGGATGTG GTGGCTGTGG TCAACGACAC GGTGGGCACC 
ATGATGACCT GTGCTTATGA GGAGCCCACC TGCGAGGTTG GACTCATCGT AGGGACTGGC 
AGCAATGCCT GCTACATGGA GGAAATGAAA AACGTCGAGA CGCTAGAAGG AAACCAGGGA 
CAAATGTGTA TCAACATGGA GTGGGGTGCC TTTGGGGACA ACGGGTGTCT GGATGATATC 
AGAACAATCT ATGACAAACT GGTGGATGAG TTTTCTCTAA ACTCTGGGAA GCAAAGGTAT 
GAGAAGATGA TCAGCGGTAT GTACCTGGGT GAGATTGTCC GCAACATCTT GATCGACTTC 
GCCAAGAGGG GCTTCCTCTT CCGGGGGCAG ATCTCTGAGC CGCTGAAGAC CCGGGGCCTC 
TTTCAGACCA AGTATCTCTC TCAGATCGAG AGCGACCGAT TAGCGCTGCT GCAGGTCCGG 
GCCATCCTCC AGCAGCTGGG CCTGAACAGC ACGTGCGACG ACAGTATCCT CGTGAAGACC 
GTGTGTGGGG TGGTGTCCAA GAGGGCGGCG CAGCTGTGTG GCGCAGGCAT GGCTGCTGTG 
GTGGATAAGA TCCGCGAAAA CAGGGGGCTG GACCGCCTCA ACGTGACTGT GGGCGTGGAC 
GGGACGCTCT ACAAGCTTCA TCCGCACTTC TCCAGAATCA TGCACCAAAC CGTGAAGGAA 
CTGTCACCAA AGTGTAATGT GTCCTTCCTC TTATCTGAAG ACGGCAGCGG CAAGGGAGCC 
GCCCTCATCA CAGCGGTGGG CGTTCGGCTC CGGCAAGAAA TGAGCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818827.1
CDS	22..2772
Translation	MDSDHSPSLPCGSAEAWETAIDKYLYAMRLSDETLLDIMNRFKKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEQNQAVHMESEVYDTPENIMHGSGSQLFDHVAECLGDFMEKKKIKDKKLPVGFTFSFPCRQSRIDEAILITWTKRFKASGVEGTDVVKLLDKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCVNTEWGAFGDDGALEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVAVQHVCTIVSFRSANLVAATLGAILSRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDCDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFSLTKEMLLEVKKRMRAEMELGLGKQTHDKAVVKMLPSFVRSTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRSVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPKMPLGFTFSFPCKQTSLDAGILITWTKGFKATDCVGHDVATLLREAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVETLEGNQGQMCINMEWGAFGDNGCLDDIRTIYDKLVDEFSLNSGKQRYEKMISGMYLGEIVRNILIDFAKRGFLFRGQISEPLKTRGLFQTKYLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRQEMSS

Target ORF information:

RefSeq Version	XM_010820525.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hexokinase 1 (HK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820525.1

ORF Insert Sequence:

ATGGACTCTG ACCACAGTCC GAGTTTGCCC TGTGGAAGTG CTGAGGCCTG GGAAACTGCA 
ATTGACAAGT ATCTCTATGC CATGCGGCTC TCTGATGAAA CTCTGTTAGA TATCATGAAT 
CGTTTCAAAA AGGAGATGAA GAATGGCCTC TCCCGGGATT TTAATCCGAC AGCCACAGTC 
AAGATGTTGC CAACGTTTGT AAGGTCTATT CCCGATGGCT CAGAAAAGGG AGATTTCATC 
GCCCTGGATC TTGGCGGATC TTCCTTTCGA ATTCTGCGGG TGCAGGTGAA TCACGAGCAG 
AACCAGGCTG TTCATATGGA GTCTGAGGTT TACGACACCC CGGAGAACAT CATGCATGGC 
AGTGGAAGCC AGCTTTTTGA TCACGTCGCT GAGTGCCTGG GAGACTTCAT GGAGAAAAAA 
AAAATCAAGG ACAAGAAATT ACCTGTGGGA TTCACGTTTT CTTTCCCTTG TCGGCAATCC 
AGAATAGACG AGGCCATCCT GATCACCTGG ACAAAGAGAT TTAAAGCGAG CGGCGTGGAG 
GGAACTGATG TGGTGAAGCT GCTTGACAAA GCCATCAAAA AGCGTGGGGA CTATGATGCC 
AACATTGTGG CTGTGGTGAA CGACACGGTG GGGACCATGA TGACCTGTGG CTACGACGAC 
CAGCAGTGTG AAGTCGGCCT CATCATTGGT ACCGGCACCA ACGCGTGCTA CATGGAGGAG 
CTGAGACACA TCGACCTGGT GGAGGGCGAC GAGGGGAGGA TGTGCGTCAA CACCGAGTGG 
GGGGCCTTTG GGGACGACGG GGCCCTGGAG GACATCCGCA CCGAGTTCGA CCGGGAGATA 
GACCGGGGGT CGCTCAACCC CGGGAAGCAG CTGTTTGAGA AGATGGTCAG TGGCATGTAC 
CTGGGCGAGC TGGTCCGACT GATCCTGGTC AAGATGGCCA AGGAGGGCCT CTTATTTGAA 
GGGCGGATCA CCCCAGAGCT GCTCACCAGA GGGAAGTTTA ACACCAGTGA CGTGTCGGCC 
ATCGAAAAGA ATAAGGAAGG CCTCCACAAT GCCAAAGAAA TCCTGACCCG CCTGGGAGTG 
GAGCCGTCTG ATGACGACTG TGTTGCGGTT CAGCATGTGT GCACCATCGT CTCATTTCGC 
TCTGCCAACC TGGTGGCGGC GACCCTGGGT GCCATCTTGA GCCGCCTTCG AGATAACAAG 
GGCACGCCCA GGCTGCGGAC CACGGTTGGT GTCGACGGGT CCCTTTACAA GACGCACCCA 
CAGTATTCTC GGCGTTTCCA CAAGACTCTG CGGCGCTTGG TACCCGACTG TGATGTGCGT 
TTCCTCCTCT CCGAGAGCGG CAGTGGCAAG GGGGCCGCCA TGGTGACTGC CGTGGCCTAC 
CGCCTGGCTG AGCAGCACCG GCAGATAGAG GAGACCCTGG CCCACTTCAG CCTCACCAAG 
GAAATGCTGC TGGAGGTGAA AAAGCGGATG CGGGCTGAGA TGGAATTGGG GCTGGGCAAG 
CAGACGCATG ACAAGGCTGT GGTCAAGATG TTGCCCTCCT TCGTCCGGAG CACTCCTGAT 
GGGACTGAGA ACGGTGACTT CTTGGCCCTG GATCTTGGAG GAACGAATTT CCGCGTCCTG 
CTGGTGAAAA TCCGTAGTGG GAAAAAGAGA TCGGTGGAAA TGCACAATAA GATCTACGCC 
ATTCCCATTG AAATCATGCA GGGCACTGGG GAGGAGCTGT TTGACCACAT CGTTTCCTGC 
ATCTCTGACT TCCTGGACTA CATGGGCATC AAGGGCCCTA AAATGCCTCT GGGCTTCACA 
TTTTCGTTTC CCTGCAAGCA GACGAGCTTA GATGCGGGTA TCTTAATCAC CTGGACCAAA 
GGTTTTAAGG CAACCGACTG CGTGGGGCAT GATGTAGCAA CCTTACTGAG GGAGGCGATA 
AAAAGGAGAG AGGAATTTGA CCTGGATGTG GTGGCTGTGG TCAACGACAC GGTGGGCACC 
ATGATGACCT GTGCTTATGA GGAGCCCACC TGCGAGGTTG GACTCATCGT AGGGACTGGC 
AGCAATGCCT GCTACATGGA GGAAATGAAA AACGTCGAGA CGCTAGAAGG AAACCAGGGA 
CAAATGTGTA TCAACATGGA GTGGGGTGCC TTTGGGGACA ACGGGTGTCT GGATGATATC 
AGAACAATCT ATGACAAACT GGTGGATGAG TTTTCTCTAA ACTCTGGGAA GCAAAGGTAT 
GAGAAGATGA TCAGCGGTAT GTACCTGGGT GAGATTGTCC GCAACATCTT GATCGACTTC 
GCCAAGAGGG GCTTCCTCTT CCGGGGGCAG ATCTCTGAGC CGCTGAAGAC CCGGGGCCTC 
TTTCAGACCA AGTATCTCTC TCAGATCGAG AGCGACCGAT TAGCGCTGCT GCAGGTCCGG 
GCCATCCTCC AGCAGCTGGG CCTGAACAGC ACGTGCGACG ACAGTATCCT CGTGAAGACC 
GTGTGTGGGG TGGTGTCCAA GAGGGCGGCG CAGCTGTGTG GCGCAGGCAT GGCTGCTGTG 
GTGGATAAGA TCCGCGAAAA CAGGGGGCTG GACCGCCTCA ACGTGACTGT GGGCGTGGAC 
GGGACGCTCT ACAAGCTTCA TCCGCACTTC TCCAGAATCA TGCACCAAAC CGTGAAGGAA 
CTGTCACCAA AGTGTAATGT GTCCTTCCTC TTATCTGAAG ACGGCAGCGG CAAGGGAGCC 
GCCCTCATCA CAGCGGTGGG CGTTCGGCTC CGGCAAGAAA TGAGCAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK1 ( NM_001012668.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001012686.1 Bos taurus hexokinase 1 (HK1), mRNA.

Price & Availability↑

CloneID	OBa239207
Clone ID Related Accession (Same CDS sequence)	NM_001012668.2
Accession Version	NM_001012668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Bos taurus(cattle)
Product	hexokinase-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000028.1. On Jan 20, 2018 this sequence version replaced NM_001012668.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF542053.1, SRR6764281.28776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATCGCCG CGCAGCTCCT AGCTTATTAC TTCACCGAAC TGAAGGATGA CCAAGTCAAA 
AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCTGATG AAACTCTGTT AGATATCATG 
AATCGTTTCA AAAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC GACAGCCACA 
GTCAAGATGT TGCCAACGTT TGTAAGGTCT ATTCCCGATG GCTCAGAAAA GGGAGATTTC 
ATCGCCCTGG ATCTTGGCGG ATCTTCCTTT CGAATTCTGC GGGTGCAGGT GAATCACGAG 
CAGAACCAGG CTGTTCATAT GGAGTCTGAG GTTTACGACA CCCCGGAGAA CATCATGCAT 
GGCAGTGGAA GCCAGCTTTT TGATCACGTC GCTGAGTGCC TGGGAGACTT CATGGAGAAA 
AAAAAAATCA AGGACAAGAA ATTACCTGTG GGATTCACGT TTTCTTTCCC TTGTCGGCAA 
TCCAGAATAG ACGAGGCCAT CCTGATCACC TGGACAAAGA GATTTAAAGC GAGCGGCGTG 
GAGGGAACTG ATGTGGTGAA GCTGCTTGAC AAAGCCATCA AAAAGCGTGG GGACTATGAT 
GCCAACATTG TGGCTGTGGT GAACGACACG GTGGGGACCA TGATGACCTG TGGCTACGAC 
GACCAGCAGT GTGAAGTCGG CCTCATCATT GGTACCGGCA CCAACGCGTG CTACATGGAG 
GAGCTGAGAC ACATCGACCT GGTGGAGGGC GACGAGGGGA GGATGTGCGT CAACACCGAG 
TGGGGGGCCT TTGGGGACGA CGGGGCCCTG GAGGACATCC GCACCGAGTT CGACCGGGAG 
ATAGACCGGG GGTCGCTCAA CCCCGGGAAG CAGCTGTTTG AGAAGATGGT CAGTGGCATG 
TACCTGGGCG AGCTGGTCCG ACTGATCCTG GTCAAGATGG CCAAGGAGGG CCTCTTATTT 
GAAGGGCGGA TCACCCCAGA GCTGCTCACC AGAGGGAAGT TTAACACCAG TGACGTGTCG 
GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA 
GTGGAGCCGT CTGATGACGA CTGTGTTGCG GTTCAGCATG TGTGCACCAT CGTCTCATTT 
CGCTCTGCCA ACCTGGTGGC GGCGACCCTG GGTGCCATCT TGAGCCGCCT TCGAGATAAC 
AAGGGCACGC CCAGGCTGCG GACCACGGTT GGTGTCGACG GGTCCCTTTA CAAGACGCAC 
CCACAGTATT CTCGGCGTTT CCACAAGACT CTGCGGCGCT TGGTACCCGA CTGTGATGTG 
CGTTTCCTCC TCTCCGAGAG CGGCAGTGGC AAGGGGGCCG CCATGGTGAC TGCCGTGGCC 
TACCGCCTGG CTGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCCCACTT CAGCCTCACC 
AAGGAAATGC TGCTGGAGGT GAAAAAGCGG ATGCGGGCTG AGATGGAATT GGGGCTGGGC 
AAGCAGACGC ATGACAAGGC TGTGGTCAAG ATGTTGCCCT CCTTCGTCCG GAGCACTCCT 
GATGGGACTG AGAACGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACGAA TTTCCGCGTC 
CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGATCGGTGG AAATGCACAA TAAGATCTAC 
GCCATTCCCA TTGAAATCAT GCAGGGCACT GGGGAGGAGC TGTTTGACCA CATCGTTTCC 
TGCATCTCTG ACTTCCTGGA CTACATGGGC ATCAAGGGCC CTAAAATGCC TCTGGGCTTC 
ACATTTTCGT TTCCCTGCAA GCAGACGAGC TTAGATGCGG GTATCTTAAT CACCTGGACC 
AAAGGTTTTA AGGCAACCGA CTGCGTGGGG CATGATGTAG CAACCTTACT GAGGGAGGCG 
ATAAAAAGGA GAGAGGAATT TGACCTGGAT GTGGTGGCTG TGGTCAACGA CACGGTGGGC 
ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGCGAGG TTGGACTCAT CGTAGGGACT 
GGCAGCAATG CCTGCTACAT GGAGGAAATG AAAAACGTCG AGACGCTAGA AGGAAACCAG 
GGACAAATGT GTATCAACAT GGAGTGGGGT GCCTTTGGGG ACAACGGGTG TCTGGATGAT 
ATCAGAACAA TCTATGACAA ACTGGTGGAT GAGTTTTCTC TAAACTCTGG GAAGCAAAGG 
TATGAGAAGA TGATCAGCGG TATGTACCTG GGTGAGATTG TCCGCAACAT CTTGATCGAC 
TTCGCCAAGA GGGGCTTCCT CTTCCGGGGG CAGATCTCTG AGCCGCTGAA GACCCGGGGC 
CTCTTTCAGA CCAAGTATCT CTCTCAGATC GAGAGCGACC GATTAGCGCT GCTGCAGGTC 
CGGGCCATCC TCCAGCAGCT GGGCCTGAAC AGCACGTGCG ACGACAGTAT CCTCGTGAAG 
ACCGTGTGTG GGGTGGTGTC CAAGAGGGCG GCGCAGCTGT GTGGCGCAGG CATGGCTGCT 
GTGGTGGATA AGATCCGCGA AAACAGGGGG CTGGACCGCC TCAACGTGAC TGTGGGCGTG 
GACGGGACGC TCTACAAGCT TCATCCGCAC TTCTCCAGAA TCATGCACCA AACCGTGAAG 
GAACTGTCAC CAAAGTGTAA TGTGTCCTTC CTCTTATCTG AAGACGGCAG CGGCAAGGGA 
GCCGCCCTCA TCACAGCGGT GGGCGTTCGG CTCCGGCAAG AAATGAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012686.1
CDS	118..2871
Translation	MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLLDIMNRFKKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEQNQAVHMESEVYDTPENIMHGSGSQLFDHVAECLGDFMEKKKIKDKKLPVGFTFSFPCRQSRIDEAILITWTKRFKASGVEGTDVVKLLDKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCVNTEWGAFGDDGALEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVAVQHVCTIVSFRSANLVAATLGAILSRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDCDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFSLTKEMLLEVKKRMRAEMELGLGKQTHDKAVVKMLPSFVRSTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRSVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPKMPLGFTFSFPCKQTSLDAGILITWTKGFKATDCVGHDVATLLREAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVETLEGNQGQMCINMEWGAFGDNGCLDDIRTIYDKLVDEFSLNSGKQRYEKMISGMYLGEIVRNILIDFAKRGFLFRGQISEPLKTRGLFQTKYLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRQEMSS

Target ORF information:

RefSeq Version	NM_001012668.2
Organism	Bos taurus(cattle)
Definition	Bos taurus hexokinase 1 (HK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012668.2

ORF Insert Sequence:

ATGATCGCCG CGCAGCTCCT AGCTTATTAC TTCACCGAAC TGAAGGATGA CCAAGTCAAA 
AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCTGATG AAACTCTGTT AGATATCATG 
AATCGTTTCA AAAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC GACAGCCACA 
GTCAAGATGT TGCCAACGTT TGTAAGGTCT ATTCCCGATG GCTCAGAAAA GGGAGATTTC 
ATCGCCCTGG ATCTTGGCGG ATCTTCCTTT CGAATTCTGC GGGTGCAGGT GAATCACGAG 
CAGAACCAGG CTGTTCATAT GGAGTCTGAG GTTTACGACA CCCCGGAGAA CATCATGCAT 
GGCAGTGGAA GCCAGCTTTT TGATCACGTC GCTGAGTGCC TGGGAGACTT CATGGAGAAA 
AAAAAAATCA AGGACAAGAA ATTACCTGTG GGATTCACGT TTTCTTTCCC TTGTCGGCAA 
TCCAGAATAG ACGAGGCCAT CCTGATCACC TGGACAAAGA GATTTAAAGC GAGCGGCGTG 
GAGGGAACTG ATGTGGTGAA GCTGCTTGAC AAAGCCATCA AAAAGCGTGG GGACTATGAT 
GCCAACATTG TGGCTGTGGT GAACGACACG GTGGGGACCA TGATGACCTG TGGCTACGAC 
GACCAGCAGT GTGAAGTCGG CCTCATCATT GGTACCGGCA CCAACGCGTG CTACATGGAG 
GAGCTGAGAC ACATCGACCT GGTGGAGGGC GACGAGGGGA GGATGTGCGT CAACACCGAG 
TGGGGGGCCT TTGGGGACGA CGGGGCCCTG GAGGACATCC GCACCGAGTT CGACCGGGAG 
ATAGACCGGG GGTCGCTCAA CCCCGGGAAG CAGCTGTTTG AGAAGATGGT CAGTGGCATG 
TACCTGGGCG AGCTGGTCCG ACTGATCCTG GTCAAGATGG CCAAGGAGGG CCTCTTATTT 
GAAGGGCGGA TCACCCCAGA GCTGCTCACC AGAGGGAAGT TTAACACCAG TGACGTGTCG 
GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA 
GTGGAGCCGT CTGATGACGA CTGTGTTGCG GTTCAGCATG TGTGCACCAT CGTCTCATTT 
CGCTCTGCCA ACCTGGTGGC GGCGACCCTG GGTGCCATCT TGAGCCGCCT TCGAGATAAC 
AAGGGCACGC CCAGGCTGCG GACCACGGTT GGTGTCGACG GGTCCCTTTA CAAGACGCAC 
CCACAGTATT CTCGGCGTTT CCACAAGACT CTGCGGCGCT TGGTACCCGA CTGTGATGTG 
CGTTTCCTCC TCTCCGAGAG CGGCAGTGGC AAGGGGGCCG CCATGGTGAC TGCCGTGGCC 
TACCGCCTGG CTGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCCCACTT CAGCCTCACC 
AAGGAAATGC TGCTGGAGGT GAAAAAGCGG ATGCGGGCTG AGATGGAATT GGGGCTGGGC 
AAGCAGACGC ATGACAAGGC TGTGGTCAAG ATGTTGCCCT CCTTCGTCCG GAGCACTCCT 
GATGGGACTG AGAACGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACGAA TTTCCGCGTC 
CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGATCGGTGG AAATGCACAA TAAGATCTAC 
GCCATTCCCA TTGAAATCAT GCAGGGCACT GGGGAGGAGC TGTTTGACCA CATCGTTTCC 
TGCATCTCTG ACTTCCTGGA CTACATGGGC ATCAAGGGCC CTAAAATGCC TCTGGGCTTC 
ACATTTTCGT TTCCCTGCAA GCAGACGAGC TTAGATGCGG GTATCTTAAT CACCTGGACC 
AAAGGTTTTA AGGCAACCGA CTGCGTGGGG CATGATGTAG CAACCTTACT GAGGGAGGCG 
ATAAAAAGGA GAGAGGAATT TGACCTGGAT GTGGTGGCTG TGGTCAACGA CACGGTGGGC 
ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGCGAGG TTGGACTCAT CGTAGGGACT 
GGCAGCAATG CCTGCTACAT GGAGGAAATG AAAAACGTCG AGACGCTAGA AGGAAACCAG 
GGACAAATGT GTATCAACAT GGAGTGGGGT GCCTTTGGGG ACAACGGGTG TCTGGATGAT 
ATCAGAACAA TCTATGACAA ACTGGTGGAT GAGTTTTCTC TAAACTCTGG GAAGCAAAGG 
TATGAGAAGA TGATCAGCGG TATGTACCTG GGTGAGATTG TCCGCAACAT CTTGATCGAC 
TTCGCCAAGA GGGGCTTCCT CTTCCGGGGG CAGATCTCTG AGCCGCTGAA GACCCGGGGC 
CTCTTTCAGA CCAAGTATCT CTCTCAGATC GAGAGCGACC GATTAGCGCT GCTGCAGGTC 
CGGGCCATCC TCCAGCAGCT GGGCCTGAAC AGCACGTGCG ACGACAGTAT CCTCGTGAAG 
ACCGTGTGTG GGGTGGTGTC CAAGAGGGCG GCGCAGCTGT GTGGCGCAGG CATGGCTGCT 
GTGGTGGATA AGATCCGCGA AAACAGGGGG CTGGACCGCC TCAACGTGAC TGTGGGCGTG 
GACGGGACGC TCTACAAGCT TCATCCGCAC TTCTCCAGAA TCATGCACCA AACCGTGAAG 
GAACTGTCAC CAAAGTGTAA TGTGTCCTTC CTCTTATCTG AAGACGGCAG CGGCAAGGGA 
GCCGCCCTCA TCACAGCGGT GGGCGTTCGG CTCCGGCAAG AAATGAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK1 ( NM_001012668.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001012686.1 Bos taurus hexokinase 1 (HK1), mRNA.

Price & Availability↑

CloneID	OBa41322
Clone ID Related Accession (Same CDS sequence)	NM_001012668.1
Accession Version	NM_001012668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Bos taurus(cattle)
Product	hexokinase-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF542053.1. On or before Aug 1, 2012 this sequence version replaced NM_174319.1, XM_601253.1, XM_617743.1. ##Evidence-Data-START## Transcript exon combination :: AF542053.1, BC114090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATCGCCG CGCAGCTCCT AGCTTATTAC TTCACCGAAC TGAAGGATGA CCAAGTCAAA 
AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCTGATG AAACTCTGTT AGATATCATG 
AATCGTTTCA AAAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC GACAGCCACA 
GTCAAGATGT TGCCAACGTT TGTAAGGTCT ATTCCCGATG GCTCAGAAAA GGGAGATTTC 
ATCGCCCTGG ATCTTGGCGG ATCTTCCTTT CGAATTCTGC GGGTGCAGGT GAATCACGAG 
CAGAACCAGG CTGTTCATAT GGAGTCTGAG GTTTACGACA CCCCGGAGAA CATCATGCAT 
GGCAGTGGAA GCCAGCTTTT TGATCACGTC GCTGAGTGCC TGGGAGACTT CATGGAGAAA 
AAAAAAATCA AGGACAAGAA ATTACCTGTG GGATTCACGT TTTCTTTCCC TTGTCGGCAA 
TCCAGAATAG ACGAGGCCAT CCTGATCACC TGGACAAAGA GATTTAAAGC GAGCGGCGTG 
GAGGGAACTG ATGTGGTGAA GCTGCTTGAC AAAGCCATCA AAAAGCGTGG GGACTATGAT 
GCCAACATTG TGGCTGTGGT GAACGACACG GTGGGGACCA TGATGACCTG TGGCTACGAC 
GACCAGCAGT GTGAAGTCGG CCTCATCATT GGTACCGGCA CCAACGCGTG CTACATGGAG 
GAGCTGAGAC ACATCGACCT GGTGGAGGGC GACGAGGGGA GGATGTGCGT CAACACCGAG 
TGGGGGGCCT TTGGGGACGA CGGGGCCCTG GAGGACATCC GCACCGAGTT CGACCGGGAG 
ATAGACCGGG GGTCGCTCAA CCCCGGGAAG CAGCTGTTTG AGAAGATGGT CAGTGGCATG 
TACCTGGGCG AGCTGGTCCG ACTGATCCTG GTCAAGATGG CCAAGGAGGG CCTCTTATTT 
GAAGGGCGGA TCACCCCAGA GCTGCTCACC AGAGGGAAGT TTAACACCAG TGACGTGTCG 
GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA 
GTGGAGCCGT CTGATGACGA CTGTGTTGCG GTTCAGCAYG TGTGCACCAT CGTCTCATTT 
CGCTCTGCCA ACCTGGTGGC GGCGACCCTG GGTGCCATCT TGAGCCGCCT TCGAGATAAC 
AAGGGCACGC CCAGGCTGCG GACCACGGTT GGTGTCGACG GGTCCCTTTA CAAGACGCAC 
CCACAGTATT CTCGGCGTTT CCACAAGACT CTGCGGCGCT TGGTACCCGA CTGTGATGTG 
CGTTTCCTCC TCTCCGAGAG CGGCAGTGGC AAGGGGGCCG CCATGGTGAC TGCCGTGGCC 
TACCGCCTGG CTGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCCCACTT CAGCCTCACC 
AAGGAAATGC TGCTGGAGGT GAAAAAGCGG ATGCGGGCTG AGATGGAATT GGGGCTGGGC 
AAGCAGACGC ATGACAAGGC TGTGGTCAAG ATGTTGCCCT CCTTCGTCCG GAGCACTCCT 
GATGGGACTG AGAACGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACGAA TTTCCGCGTC 
CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGATCGGTGG AAATGCACAA TAAGATCTAC 
GCCATTCCCA TTGAAATCAT GCAGGGCACT GGGGAGGAGC TGTTTGACCA CATCGTTTCC 
TGCATCTCTG ACTTCCTGGA CTACATGGGC ATCAAGGGCC CTAAAATGCC TCTGGGCTTC 
ACATTTTCGT TTCCCTGCAA GCAGACGAGC TTAGATGCGG GTATCTTAAT CACCTGGACC 
AAAGGTTTTA AGGCAACCGA CTGCGTGGGG CATGATGTAG CAACCTTACT GAGGGAGGCG 
ATAAAAAGGA GAGAGGAATT TGACCTGGAT GTGGTGGCTG TGGTCAACGA CACGGTGGGC 
ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGCGAGG TTGGACTCAT CGTAGGGACT 
GGCAGCAATG CCTGCTACAT GGAGGAAATG AAAAACGTCG AGACGCTAGA AGGAAACCAG 
GGACAAATGT GTATCAACAT GGAGTGGGGT GCCTTTGGGG ACAACGGGTG TCTGGATGAT 
ATCAGAACAA TCTATGACAA ACTGGTGGAT GAGTTTTCTC TAAACTCTGG GAAGCAAAGG 
TATGAGAAGA TGATCAGCGG TATGTACCTG GGTGAGATTG TCCGCAACAT CTTGATCGAC 
TTCGCCAAGA GGGGCTTCCT CTTCCGGGGG CAGATCTCTG AGCCGCTGAA GACCCGGGGC 
CTCTTTCAGA CCAAGTATCT CTCTCAGATC GAGAGCGACC GATTAGCGCT GCTGCAGGTC 
CGGGCCATCC TCCAGCAGCT GGGCCTGAAC AGCACGTGCG ACGACAGTAT CCTCGTGAAG 
ACCGTGTGTG GGGTGGTGTC CAAGAGGGCG GCGCAGCTGT GTGGCGCAGG CATGGCTGCT 
GTGGTGGATA AGATCCGCGA AAACAGGGGG CTGGACCGCC TCAACGTGAC TGTGGGCGTG 
GACGGGACGC TCTACAAGCT TCATCCGCAC TTCTCCAGAA TCATGCACCA AACCGTGAAG 
GAACTGTCAC CAAAGTGTAA TGTGTCCTTC CTCTTATCTG AAGACGGCAG CGGCAAGGGA 
GCCGCCCTCA TCACAGCGGT GGGCGTTCGG CTCCGGCAAG AAATGAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012686.1
CDS	118..2871
Translation	MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLLDIMNRFKKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEQNQAVHMESEVYDTPENIMHGSGSQLFDHVAECLGDFMEKKKIKDKKLPVGFTFSFPCRQSRIDEAILITWTKRFKASGVEGTDVVKLLDKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCVNTEWGAFGDDGALEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVAVQnullVCTIVSFRSANLVAATLGAILSRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDCDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFSLTKEMLLEVKKRMRAEMELGLGKQTHDKAVVKMLPSFVRSTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRSVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPKMPLGFTFSFPCKQTSLDAGILITWTKGFKATDCVGHDVATLLREAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVETLEGNQGQMCINMEWGAFGDNGCLDDIRTIYDKLVDEFSLNSGKQRYEKMISGMYLGEIVRNILIDFAKRGFLFRGQISEPLKTRGLFQTKYLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRQEMSS

Target ORF information:

RefSeq Version	NM_001012668.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hexokinase 1 (HK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012668.1

ORF Insert Sequence:

ATGATCGCCG CGCAGCTCCT AGCTTATTAC TTCACCGAAC TGAAGGATGA CCAAGTCAAA 
AAGATTGACA AGTATCTCTA TGCCATGCGG CTCTCTGATG AAACTCTGTT AGATATCATG 
AATCGTTTCA AAAAGGAGAT GAAGAATGGC CTCTCCCGGG ATTTTAATCC GACAGCCACA 
GTCAAGATGT TGCCAACGTT TGTAAGGTCT ATTCCCGATG GCTCAGAAAA GGGAGATTTC 
ATCGCCCTGG ATCTTGGCGG ATCTTCCTTT CGAATTCTGC GGGTGCAGGT GAATCACGAG 
CAGAACCAGG CTGTTCATAT GGAGTCTGAG GTTTACGACA CCCCGGAGAA CATCATGCAT 
GGCAGTGGAA GCCAGCTTTT TGATCACGTC GCTGAGTGCC TGGGAGACTT CATGGAGAAA 
AAAAAAATCA AGGACAAGAA ATTACCTGTG GGATTCACGT TTTCTTTCCC TTGTCGGCAA 
TCCAGAATAG ACGAGGCCAT CCTGATCACC TGGACAAAGA GATTTAAAGC GAGCGGCGTG 
GAGGGAACTG ATGTGGTGAA GCTGCTTGAC AAAGCCATCA AAAAGCGTGG GGACTATGAT 
GCCAACATTG TGGCTGTGGT GAACGACACG GTGGGGACCA TGATGACCTG TGGCTACGAC 
GACCAGCAGT GTGAAGTCGG CCTCATCATT GGTACCGGCA CCAACGCGTG CTACATGGAG 
GAGCTGAGAC ACATCGACCT GGTGGAGGGC GACGAGGGGA GGATGTGCGT CAACACCGAG 
TGGGGGGCCT TTGGGGACGA CGGGGCCCTG GAGGACATCC GCACCGAGTT CGACCGGGAG 
ATAGACCGGG GGTCGCTCAA CCCCGGGAAG CAGCTGTTTG AGAAGATGGT CAGTGGCATG 
TACCTGGGCG AGCTGGTCCG ACTGATCCTG GTCAAGATGG CCAAGGAGGG CCTCTTATTT 
GAAGGGCGGA TCACCCCAGA GCTGCTCACC AGAGGGAAGT TTAACACCAG TGACGTGTCG 
GCCATCGAAA AGAATAAGGA AGGCCTCCAC AATGCCAAAG AAATCCTGAC CCGCCTGGGA 
GTGGAGCCGT CTGATGACGA CTGTGTTGCG GTTCAGCAYG TGTGCACCAT CGTCTCATTT 
CGCTCTGCCA ACCTGGTGGC GGCGACCCTG GGTGCCATCT TGAGCCGCCT TCGAGATAAC 
AAGGGCACGC CCAGGCTGCG GACCACGGTT GGTGTCGACG GGTCCCTTTA CAAGACGCAC 
CCACAGTATT CTCGGCGTTT CCACAAGACT CTGCGGCGCT TGGTACCCGA CTGTGATGTG 
CGTTTCCTCC TCTCCGAGAG CGGCAGTGGC AAGGGGGCCG CCATGGTGAC TGCCGTGGCC 
TACCGCCTGG CTGAGCAGCA CCGGCAGATA GAGGAGACCC TGGCCCACTT CAGCCTCACC 
AAGGAAATGC TGCTGGAGGT GAAAAAGCGG ATGCGGGCTG AGATGGAATT GGGGCTGGGC 
AAGCAGACGC ATGACAAGGC TGTGGTCAAG ATGTTGCCCT CCTTCGTCCG GAGCACTCCT 
GATGGGACTG AGAACGGTGA CTTCTTGGCC CTGGATCTTG GAGGAACGAA TTTCCGCGTC 
CTGCTGGTGA AAATCCGTAG TGGGAAAAAG AGATCGGTGG AAATGCACAA TAAGATCTAC 
GCCATTCCCA TTGAAATCAT GCAGGGCACT GGGGAGGAGC TGTTTGACCA CATCGTTTCC 
TGCATCTCTG ACTTCCTGGA CTACATGGGC ATCAAGGGCC CTAAAATGCC TCTGGGCTTC 
ACATTTTCGT TTCCCTGCAA GCAGACGAGC TTAGATGCGG GTATCTTAAT CACCTGGACC 
AAAGGTTTTA AGGCAACCGA CTGCGTGGGG CATGATGTAG CAACCTTACT GAGGGAGGCG 
ATAAAAAGGA GAGAGGAATT TGACCTGGAT GTGGTGGCTG TGGTCAACGA CACGGTGGGC 
ACCATGATGA CCTGTGCTTA TGAGGAGCCC ACCTGCGAGG TTGGACTCAT CGTAGGGACT 
GGCAGCAATG CCTGCTACAT GGAGGAAATG AAAAACGTCG AGACGCTAGA AGGAAACCAG 
GGACAAATGT GTATCAACAT GGAGTGGGGT GCCTTTGGGG ACAACGGGTG TCTGGATGAT 
ATCAGAACAA TCTATGACAA ACTGGTGGAT GAGTTTTCTC TAAACTCTGG GAAGCAAAGG 
TATGAGAAGA TGATCAGCGG TATGTACCTG GGTGAGATTG TCCGCAACAT CTTGATCGAC 
TTCGCCAAGA GGGGCTTCCT CTTCCGGGGG CAGATCTCTG AGCCGCTGAA GACCCGGGGC 
CTCTTTCAGA CCAAGTATCT CTCTCAGATC GAGAGCGACC GATTAGCGCT GCTGCAGGTC 
CGGGCCATCC TCCAGCAGCT GGGCCTGAAC AGCACGTGCG ACGACAGTAT CCTCGTGAAG 
ACCGTGTGTG GGGTGGTGTC CAAGAGGGCG GCGCAGCTGT GTGGCGCAGG CATGGCTGCT 
GTGGTGGATA AGATCCGCGA AAACAGGGGG CTGGACCGCC TCAACGTGAC TGTGGGCGTG 
GACGGGACGC TCTACAAGCT TCATCCGCAC TTCTCCAGAA TCATGCACCA AACCGTGAAG 
GAACTGTCAC CAAAGTGTAA TGTGTCCTTC CTCTTATCTG AAGACGGCAG CGGCAAGGGA 
GCCGCCCTCA TCACAGCGGT GGGCGTTCGG CTCCGGCAAG AAATGAGCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK1 ( XM_024986501.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842269.1 Bos taurus hexokinase 1 (HK1), transcript variant X1, mRNA.

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CloneID	OBa45016
Clone ID Related Accession (Same CDS sequence)	XM_024986501.1
Accession Version	XM_024986501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hexokinase-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGA CCTGCCAGCG AGGATGGGGT ACAGAAACTG AAAATTCAAT CCAGCCCCCA 
CTTACTGAAA GTAAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCTGA TGAAACTCTG 
TTAGATATCA TGAATCGTTT CAAAAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT 
CCGACAGCCA CAGTCAAGAT GTTGCCAACG TTTGTAAGGT CTATTCCCGA TGGCTCAGAA 
AAGGGAGATT TCATCGCCCT GGATCTTGGC GGATCTTCCT TTCGAATTCT GCGGGTGCAG 
GTGAATCACG AGCAGAACCA GGCTGTTCAT ATGGAGTCTG AGGTTTACGA CACCCCGGAG 
AACATCATGC ATGGCAGTGG AAGCCAGCTT TTTGATCACG TCGCTGAGTG CCTGGGAGAC 
TTCATGGAGA AAAAAAAAAT CAAGGACAAG AAATTACCTG TGGGATTCAC GTTTTCTTTC 
CCTTGTCGGC AATCCAGAAT AGACGAGGCC ATCCTGATCA CCTGGACAAA GAGATTTAAA 
GCGAGCGGCG TGGAGGGAAC TGATGTGGTG AAGCTGCTTG ACAAAGCCAT CAAAAAGCGT 
GGGGACTATG ATGCCAACAT TGTGGCTGTG GTGAACGACA CGGTGGGGAC CATGATGACC 
TGTGGCTACG ACGACCAGCA GTGTGAAGTC GGCCTCATCA TTGGTACCGG CACCAACGCG 
TGCTACATGG AGGAGCTGAG ACACATCGAC CTGGTGGAGG GCGACGAGGG GAGGATGTGC 
GTCAACACCG AGTGGGGGGC CTTTGGGGAC GACGGGGCCC TGGAGGACAT CCGCACCGAG 
TTCGACCGGG AGATAGACCG GGGGTCGCTC AACCCCGGGA AGCAGCTGTT TGAGAAGATG 
GTCAGTGGCA TGTACCTGGG CGAGCTGGTC CGACTGATCC TGGTCAAGAT GGCCAAGGAG 
GGCCTCTTAT TTGAAGGGCG GATCACCCCA GAGCTGCTCA CCAGAGGGAA GTTTAACACC 
AGTGACGTGT CGGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG 
ACCCGCCTGG GAGTGGAGCC GTCTGATGAC GACTGTGTTG CGGTTCAGCA TGTGTGCACC 
ATCGTCTCAT TTCGCTCTGC CAACCTGGTG GCGGCGACCC TGGGTGCCAT CTTGAGCCGC 
CTTCGAGATA ACAAGGGCAC GCCCAGGCTG CGGACCACGG TTGGTGTCGA CGGGTCCCTT 
TACAAGACGC ACCCACAGTA TTCTCGGCGT TTCCACAAGA CTCTGCGGCG CTTGGTACCC 
GACTGTGATG TGCGTTTCCT CCTCTCCGAG AGCGGCAGTG GCAAGGGGGC CGCCATGGTG 
ACTGCCGTGG CCTACCGCCT GGCTGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCCCAC 
TTCAGCCTCA CCAAGGAAAT GCTGCTGGAG GTGAAAAAGC GGATGCGGGC TGAGATGGAA 
TTGGGGCTGG GCAAGCAGAC GCATGACAAG GCTGTGGTCA AGATGTTGCC CTCCTTCGTC 
CGGAGCACTC CTGATGGGAC TGAGAACGGT GACTTCTTGG CCCTGGATCT TGGAGGAACG 
AATTTCCGCG TCCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGATCGGT GGAAATGCAC 
AATAAGATCT ACGCCATTCC CATTGAAATC ATGCAGGGCA CTGGGGAGGA GCTGTTTGAC 
CACATCGTTT CCTGCATCTC TGACTTCCTG GACTACATGG GCATCAAGGG CCCTAAAATG 
CCTCTGGGCT TCACATTTTC GTTTCCCTGC AAGCAGACGA GCTTAGATGC GGGTATCTTA 
ATCACCTGGA CCAAAGGTTT TAAGGCAACC GACTGCGTGG GGCATGATGT AGCAACCTTA 
CTGAGGGAGG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ATGTGGTGGC TGTGGTCAAC 
GACACGGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGCGA GGTTGGACTC 
ATCGTAGGGA CTGGCAGCAA TGCCTGCTAC ATGGAGGAAA TGAAAAACGT CGAGACGCTA 
GAAGGAAACC AGGGACAAAT GTGTATCAAC ATGGAGTGGG GTGCCTTTGG GGACAACGGG 
TGTCTGGATG ATATCAGAAC AATCTATGAC AAACTGGTGG ATGAGTTTTC TCTAAACTCT 
GGGAAGCAAA GGTATGAGAA GATGATCAGC GGTATGTACC TGGGTGAGAT TGTCCGCAAC 
ATCTTGATCG ACTTCGCCAA GAGGGGCTTC CTCTTCCGGG GGCAGATCTC TGAGCCGCTG 
AAGACCCGGG GCCTCTTTCA GACCAAGTAT CTCTCTCAGA TCGAGAGCGA CCGATTAGCG 
CTGCTGCAGG TCCGGGCCAT CCTCCAGCAG CTGGGCCTGA ACAGCACGTG CGACGACAGT 
ATCCTCGTGA AGACCGTGTG TGGGGTGGTG TCCAAGAGGG CGGCGCAGCT GTGTGGCGCA 
GGCATGGCTG CTGTGGTGGA TAAGATCCGC GAAAACAGGG GGCTGGACCG CCTCAACGTG 
ACTGTGGGCG TGGACGGGAC GCTCTACAAG CTTCATCCGC ACTTCTCCAG AATCATGCAC 
CAAACCGTGA AGGAACTGTC ACCAAAGTGT AATGTGTCCT TCCTCTTATC TGAAGACGGC 
AGCGGCAAGG GAGCCGCCCT CATCACAGCG GTGGGCGTTC GGCTCCGGCA AGAAATGAGC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842269.1
CDS	337..3102
Translation	MGQTCQRGWGTETENSIQPPLTESKIDKYLYAMRLSDETLLDIMNRFKKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEQNQAVHMESEVYDTPENIMHGSGSQLFDHVAECLGDFMEKKKIKDKKLPVGFTFSFPCRQSRIDEAILITWTKRFKASGVEGTDVVKLLDKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCVNTEWGAFGDDGALEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVAVQHVCTIVSFRSANLVAATLGAILSRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDCDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFSLTKEMLLEVKKRMRAEMELGLGKQTHDKAVVKMLPSFVRSTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRSVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPKMPLGFTFSFPCKQTSLDAGILITWTKGFKATDCVGHDVATLLREAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVETLEGNQGQMCINMEWGAFGDNGCLDDIRTIYDKLVDEFSLNSGKQRYEKMISGMYLGEIVRNILIDFAKRGFLFRGQISEPLKTRGLFQTKYLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRQEMSS

Target ORF information:

RefSeq Version	XM_024986501.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hexokinase 1 (HK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986501.1

ORF Insert Sequence:

ATGGGGCAGA CCTGCCAGCG AGGATGGGGT ACAGAAACTG AAAATTCAAT CCAGCCCCCA 
CTTACTGAAA GTAAGATTGA CAAGTATCTC TATGCCATGC GGCTCTCTGA TGAAACTCTG 
TTAGATATCA TGAATCGTTT CAAAAAGGAG ATGAAGAATG GCCTCTCCCG GGATTTTAAT 
CCGACAGCCA CAGTCAAGAT GTTGCCAACG TTTGTAAGGT CTATTCCCGA TGGCTCAGAA 
AAGGGAGATT TCATCGCCCT GGATCTTGGC GGATCTTCCT TTCGAATTCT GCGGGTGCAG 
GTGAATCACG AGCAGAACCA GGCTGTTCAT ATGGAGTCTG AGGTTTACGA CACCCCGGAG 
AACATCATGC ATGGCAGTGG AAGCCAGCTT TTTGATCACG TCGCTGAGTG CCTGGGAGAC 
TTCATGGAGA AAAAAAAAAT CAAGGACAAG AAATTACCTG TGGGATTCAC GTTTTCTTTC 
CCTTGTCGGC AATCCAGAAT AGACGAGGCC ATCCTGATCA CCTGGACAAA GAGATTTAAA 
GCGAGCGGCG TGGAGGGAAC TGATGTGGTG AAGCTGCTTG ACAAAGCCAT CAAAAAGCGT 
GGGGACTATG ATGCCAACAT TGTGGCTGTG GTGAACGACA CGGTGGGGAC CATGATGACC 
TGTGGCTACG ACGACCAGCA GTGTGAAGTC GGCCTCATCA TTGGTACCGG CACCAACGCG 
TGCTACATGG AGGAGCTGAG ACACATCGAC CTGGTGGAGG GCGACGAGGG GAGGATGTGC 
GTCAACACCG AGTGGGGGGC CTTTGGGGAC GACGGGGCCC TGGAGGACAT CCGCACCGAG 
TTCGACCGGG AGATAGACCG GGGGTCGCTC AACCCCGGGA AGCAGCTGTT TGAGAAGATG 
GTCAGTGGCA TGTACCTGGG CGAGCTGGTC CGACTGATCC TGGTCAAGAT GGCCAAGGAG 
GGCCTCTTAT TTGAAGGGCG GATCACCCCA GAGCTGCTCA CCAGAGGGAA GTTTAACACC 
AGTGACGTGT CGGCCATCGA AAAGAATAAG GAAGGCCTCC ACAATGCCAA AGAAATCCTG 
ACCCGCCTGG GAGTGGAGCC GTCTGATGAC GACTGTGTTG CGGTTCAGCA TGTGTGCACC 
ATCGTCTCAT TTCGCTCTGC CAACCTGGTG GCGGCGACCC TGGGTGCCAT CTTGAGCCGC 
CTTCGAGATA ACAAGGGCAC GCCCAGGCTG CGGACCACGG TTGGTGTCGA CGGGTCCCTT 
TACAAGACGC ACCCACAGTA TTCTCGGCGT TTCCACAAGA CTCTGCGGCG CTTGGTACCC 
GACTGTGATG TGCGTTTCCT CCTCTCCGAG AGCGGCAGTG GCAAGGGGGC CGCCATGGTG 
ACTGCCGTGG CCTACCGCCT GGCTGAGCAG CACCGGCAGA TAGAGGAGAC CCTGGCCCAC 
TTCAGCCTCA CCAAGGAAAT GCTGCTGGAG GTGAAAAAGC GGATGCGGGC TGAGATGGAA 
TTGGGGCTGG GCAAGCAGAC GCATGACAAG GCTGTGGTCA AGATGTTGCC CTCCTTCGTC 
CGGAGCACTC CTGATGGGAC TGAGAACGGT GACTTCTTGG CCCTGGATCT TGGAGGAACG 
AATTTCCGCG TCCTGCTGGT GAAAATCCGT AGTGGGAAAA AGAGATCGGT GGAAATGCAC 
AATAAGATCT ACGCCATTCC CATTGAAATC ATGCAGGGCA CTGGGGAGGA GCTGTTTGAC 
CACATCGTTT CCTGCATCTC TGACTTCCTG GACTACATGG GCATCAAGGG CCCTAAAATG 
CCTCTGGGCT TCACATTTTC GTTTCCCTGC AAGCAGACGA GCTTAGATGC GGGTATCTTA 
ATCACCTGGA CCAAAGGTTT TAAGGCAACC GACTGCGTGG GGCATGATGT AGCAACCTTA 
CTGAGGGAGG CGATAAAAAG GAGAGAGGAA TTTGACCTGG ATGTGGTGGC TGTGGTCAAC 
GACACGGTGG GCACCATGAT GACCTGTGCT TATGAGGAGC CCACCTGCGA GGTTGGACTC 
ATCGTAGGGA CTGGCAGCAA TGCCTGCTAC ATGGAGGAAA TGAAAAACGT CGAGACGCTA 
GAAGGAAACC AGGGACAAAT GTGTATCAAC ATGGAGTGGG GTGCCTTTGG GGACAACGGG 
TGTCTGGATG ATATCAGAAC AATCTATGAC AAACTGGTGG ATGAGTTTTC TCTAAACTCT 
GGGAAGCAAA GGTATGAGAA GATGATCAGC GGTATGTACC TGGGTGAGAT TGTCCGCAAC 
ATCTTGATCG ACTTCGCCAA GAGGGGCTTC CTCTTCCGGG GGCAGATCTC TGAGCCGCTG 
AAGACCCGGG GCCTCTTTCA GACCAAGTAT CTCTCTCAGA TCGAGAGCGA CCGATTAGCG 
CTGCTGCAGG TCCGGGCCAT CCTCCAGCAG CTGGGCCTGA ACAGCACGTG CGACGACAGT 
ATCCTCGTGA AGACCGTGTG TGGGGTGGTG TCCAAGAGGG CGGCGCAGCT GTGTGGCGCA 
GGCATGGCTG CTGTGGTGGA TAAGATCCGC GAAAACAGGG GGCTGGACCG CCTCAACGTG 
ACTGTGGGCG TGGACGGGAC GCTCTACAAG CTTCATCCGC ACTTCTCCAG AATCATGCAC 
CAAACCGTGA AGGAACTGTC ACCAAAGTGT AATGTGTCCT TCCTCTTATC TGAAGACGGC 
AGCGGCAAGG GAGCCGCCCT CATCACAGCG GTGGGCGTTC GGCTCCGGCA AGAAATGAGC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Bovine hexokinase type I: full-length cDNA sequence and characterisation of the recombinant enzyme.
Molecular and cellular biochemistry268(1-2)9-18(2005 Jan)
Andreoni F,Serafini G,Laguardia ME,Magnani M

Mammalian hexokinase 1: evolutionary conservation and structure to function analysis.
Genomics11(4)1014-24(1991 Dec)
Griffin LD,Gelb BD,Wheeler DA,Davison D,Adams V,McCabe ER

Synthesis and characterization of a bovine hexokinase 1 cDNA probe by mixed oligonucleotide primed amplification of cDNA using high complexity primer mixtures.
Biochemical medicine and metabolic biology41(2)125-31(1989 Apr)
Griffin LD,MacGregor GR,Muzny DM,Harter J,Cook RG,McCabe ER

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HK1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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