gRNAs
The following HCK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa40060 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001110194.1 | |
| Accession Version | NM_001110194.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-01-22 | |
| Organism | Bos taurus(cattle) | |
| Product | tyrosine-protein kinase HCK isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000013.1. On Aug 31, 2013 this sequence version replaced XM_005214482.1. Transcript Variant: This variant (1) represents the longer and more predominant transcript. It initiates translation from an upstream non-AUG (CUG) start codon (resulting in isoform 1), and alternatively from an in-frame, downstream AUG. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.35978.1, SRR6764281.19102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 10967098, 1875927 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | CTGGGGGGGC GCTCGAGCTG CGAGGATCCG GGCTGCCCGC CGGAAGAGGA GCGGGCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001103664.1 |
| CDS | 188..1762 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001110194.1 |
1 | CTGGGGGGGC GCTCGAGCTG CGAGGATCCG GGCTGCCCGC CGGAAGAGGA GCGGGCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa40854 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001037141.3 | |
| Accession Version | NM_001037141.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-01-22 | |
| Organism | Bos taurus(cattle) | |
| Product | tyrosine-protein kinase HCK isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000013.1. On Oct 1, 2015 this sequence version replaced NM_001037141.2. Transcript Variant: This variant (2) appears to be restricted to the testis. It is missing several 5' and 3' exons found in transcript variant 1, resulting in a shorter isoform (2), with different N- and C- termini, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DQ219802.1, EV607287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAACAGA CCCTGTTAAG GTCTGGGGAG TGGTGGAGAG CGCGATCTCT GGCCACCCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001032218.2 |
| CDS | 90..920 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001037141.3 |
1 | ATGGAACAGA CCCTGTTAAG GTCTGGGGAG TGGTGGAGAG CGCGATCTCT GGCCACCCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A whole-genome assembly of the domestic cow, Bos taurus. |
Expression of hck-tr, a truncated form of the src-related tyrosine kinase hck, in bovine spermatozoa and testis. |
Bull testicular haploid germ cells express a messenger encoding for a truncated form of the protein tyrosine kinase HCK. |
Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle. |
Comparative mapping of bovine chromosome 13 by fluorescence in situ hybridization. |
