FN1 cDNA ORF clone, Bos taurus(cattle)

The following FN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227119 XM_024998217.1
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa44268 NM_001163778.1
Latest version!
Bos taurus fibronectin 1 (FN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16668 XM_005202775.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16669 XM_005202776.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16670 XM_005202777.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16671 XM_005202778.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16672 XM_005202779.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16675 XM_005202782.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16676 XM_005202783.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16677 XM_005202784.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16678 XM_005202785.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16679 XM_005202786.4
Latest version!
Bos taurus fibronectin 1 (FN1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa16669 XM_005202776.3 Bos taurus fibronectin 1 (FN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16675 XM_005202782.3 Bos taurus fibronectin 1 (FN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16677 XM_005202784.3 Bos taurus fibronectin 1 (FN1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16678 XM_005202785.3 Bos taurus fibronectin 1 (FN1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16668 XM_005202775.3 Bos taurus fibronectin 1 (FN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16679 XM_005202786.3 Bos taurus fibronectin 1 (FN1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa16671 XM_005202778.3 Bos taurus fibronectin 1 (FN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16670 XM_005202777.3 Bos taurus fibronectin 1 (FN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16672 XM_005202779.3 Bos taurus fibronectin 1 (FN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa16676 XM_005202783.3 Bos taurus fibronectin 1 (FN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227119
    Clone ID Related Accession (Same CDS sequence) XM_024998217.1
    Accession Version XM_024998217.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG TTCAAAAGAC CCCTTTCATC
    ACCAACCCTG GGTATGACAC TGGAAACGGT ATTCAGCTTC CCGGCACTTC TGGTCAGCAG
    CCCAGTCTTG GGCAACAAAT GATCTTTGAG GAGCATGGTT TTAGGCGAAC CACACCGCCC
    ACCACGGCCA CCCCCGTAAG GCATAGGCCA AGACCGTATC CGCCGAATGT AAATGAGGAG
    ATCCAAATTG GTCATGTCCC CAGGGGAGAC GTAGACCATC ATCTCTACCC TCACGTTGTG
    GGACTCAATC CAAATGCTTC TACAGGCCAA GAAGCTCTCT CTCAGACAAC CATCTCATGG
    ACCCCATTCC AGGAAAGCTC TGAGTATATC ATTTCATGTC ATCCAGTTGG CATTGATGAA
    GAACCCTTAC AGTTCCGAGT TCCTGGAACC TCTGCTAGTG CCACCTTGAC GGGCCTCACC
    AGAGGGGCCA CCTACAACAT CATAGTGGAG GCAGTAAAAG ACCAGCAGAG GCAGAAGGTT
    CGCGAGGAGG TGGTTACCGT GGGCAATTCT GTTGACCAGG GCCTGAGTCA GCCCACAGAT
    GACTCCTGCT TCGACCCCTA CACGGTCTCC CATTATGCCA TTGGAGAGGA GTGGGAGCGA
    TTGTCTGATT CTGGCTTTAA ACTCTCGTGC CAGTGCTTAG GCTTTGGCAG TGGTCATTTC
    AGATGCGATT CATCTAAATG GTGCCATGAC AATGGAGTGA ACTACAAGAT TGGAGAGAAG
    TGGGATCGTC AGGGAGAGAA TGGCCAGATG ATGAGCTGCA CGTGTCTTGG AAATGGAAAA
    GGAGAATTCA AGTGTGATCC TCATGAGGCC ACGTGCTATG ACGATGGGAA GACTTACCAC
    GTGGGAGAAC AGTGGCAGAA GGAATATCTT GGTGCCATTT GCTCCTGCAC ATGCTTTGGA
    GGCCAGCGGG GCTGGCGCTG TGACAACTGC CGCAGACCTG GGGCTGAACC CGGTAACGAA
    GGCTCCACTG CCCACTCCTA CAACCAGTAT TCCCAGAGAT ACCATCAGAG AACAAACACT
    AATGTCAACT GCCCAATTGA GTGCTTCATG CCTTTGGATG TACAGGCTGA CAGAGAAGAT
    TCCCGAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853985.1
    CDS238..7329
    Translation

    Target ORF information:

    RefSeq Version XM_024998217.1
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998217.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG TTCAAAAGAC CCCTTTCATC
    ACCAACCCTG GGTATGACAC TGGAAACGGT ATTCAGCTTC CCGGCACTTC TGGTCAGCAG
    CCCAGTCTTG GGCAACAAAT GATCTTTGAG GAGCATGGTT TTAGGCGAAC CACACCGCCC
    ACCACGGCCA CCCCCGTAAG GCATAGGCCA AGACCGTATC CGCCGAATGT AAATGAGGAG
    ATCCAAATTG GTCATGTCCC CAGGGGAGAC GTAGACCATC ATCTCTACCC TCACGTTGTG
    GGACTCAATC CAAATGCTTC TACAGGCCAA GAAGCTCTCT CTCAGACAAC CATCTCATGG
    ACCCCATTCC AGGAAAGCTC TGAGTATATC ATTTCATGTC ATCCAGTTGG CATTGATGAA
    GAACCCTTAC AGTTCCGAGT TCCTGGAACC TCTGCTAGTG CCACCTTGAC GGGCCTCACC
    AGAGGGGCCA CCTACAACAT CATAGTGGAG GCAGTAAAAG ACCAGCAGAG GCAGAAGGTT
    CGCGAGGAGG TGGTTACCGT GGGCAATTCT GTTGACCAGG GCCTGAGTCA GCCCACAGAT
    GACTCCTGCT TCGACCCCTA CACGGTCTCC CATTATGCCA TTGGAGAGGA GTGGGAGCGA
    TTGTCTGATT CTGGCTTTAA ACTCTCGTGC CAGTGCTTAG GCTTTGGCAG TGGTCATTTC
    AGATGCGATT CATCTAAATG GTGCCATGAC AATGGAGTGA ACTACAAGAT TGGAGAGAAG
    TGGGATCGTC AGGGAGAGAA TGGCCAGATG ATGAGCTGCA CGTGTCTTGG AAATGGAAAA
    GGAGAATTCA AGTGTGATCC TCATGAGGCC ACGTGCTATG ACGATGGGAA GACTTACCAC
    GTGGGAGAAC AGTGGCAGAA GGAATATCTT GGTGCCATTT GCTCCTGCAC ATGCTTTGGA
    GGCCAGCGGG GCTGGCGCTG TGACAACTGC CGCAGACCTG GGGCTGAACC CGGTAACGAA
    GGCTCCACTG CCCACTCCTA CAACCAGTAT TCCCAGAGAT ACCATCAGAG AACAAACACT
    AATGTCAACT GCCCAATTGA GTGCTTCATG CCTTTGGATG TACAGGCTGA CAGAGAAGAT
    TCCCGAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa44268
    Clone ID Related Accession (Same CDS sequence) NM_001163778.1
    Accession Version NM_001163778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product fibronectin precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ513187.1. On or before Aug 8, 2009 this sequence version replaced XM_864390.3, XM_874054.3, XM_874147.3, XM_873966.3, XM_873485.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ513187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGATG AGCTCCCCCA GCTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCCTCCAC AGTTCAAAAG
    ACCCCTTTCA TCACCAACCC TGGGTATGAC ACTGGAAACG GTATTCAGCT TCCCGGCACT
    TCTGGTCAGC AGCCCAGTCT TGGGCAACAA ATGATCTTTG AGGAGCATGG TTTTAGGCGA
    ACCACACCGC CCACCACGGC CACCCCCGTA AGGCATAGGC CAAGACCGTA TCCGCCGAAT
    GTAAATGAGG AGATCCAAAT TGGTCATGTC CCCAGGGGAG ACGTAGACCA TCATCTCTAC
    CCTCACGTTG TGGGACTCAA TCCAAATGCT TCTACAGGCC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT
    GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCTTG
    ACGGGCCTCA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCAGTAAA AGACCAGCAG
    AGGCAGAAGG TTCGCGAGGA GGTGGTTACC GTGGGCAATT CTGTTGACCA GGGCCTGAGT
    CAGCCCACAG ATGACTCCTG CTTCGACCCC TACACGGTCT CCCATTATGC CATTGGAGAG
    GAGTGGGAGC GATTGTCTGA TTCTGGCTTT AAACTCTCGT GCCAGTGCTT AGGCTTTGGC
    AGTGGTCATT TCAGATGCGA TTCATCTAAA TGGTGCCATG ACAATGGAGT GAACTACAAG
    ATTGGAGAGA AGTGGGATCG TCAGGGAGAG AATGGCCAGA TGATGAGCTG CACGTGTCTT
    GGAAATGGAA AAGGAGAATT CAAGTGTGAT CCTCATGAGG CCACGTGCTA TGACGATGGG
    AAGACTTACC ACGTGGGAGA ACAGTGGCAG AAGGAATATC TTGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA
    CCCGGTAACG AAGGCTCCAC TGCCCACTCC TACAACCAGT ATTCCCAGAG ATACCATCAG
    AGAACAAACA CTAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTGGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157250.1
    CDS198..7361
    Translation

    Target ORF information:

    RefSeq Version NM_001163778.1
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGATG AGCTCCCCCA GCTGGTAACC
    CTTCCACACC CCAATCTTCA TGGACCAGAG ATCTTGGATG TTCCCTCCAC AGTTCAAAAG
    ACCCCTTTCA TCACCAACCC TGGGTATGAC ACTGGAAACG GTATTCAGCT TCCCGGCACT
    TCTGGTCAGC AGCCCAGTCT TGGGCAACAA ATGATCTTTG AGGAGCATGG TTTTAGGCGA
    ACCACACCGC CCACCACGGC CACCCCCGTA AGGCATAGGC CAAGACCGTA TCCGCCGAAT
    GTAAATGAGG AGATCCAAAT TGGTCATGTC CCCAGGGGAG ACGTAGACCA TCATCTCTAC
    CCTCACGTTG TGGGACTCAA TCCAAATGCT TCTACAGGCC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT
    GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCTTG
    ACGGGCCTCA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCAGTAAA AGACCAGCAG
    AGGCAGAAGG TTCGCGAGGA GGTGGTTACC GTGGGCAATT CTGTTGACCA GGGCCTGAGT
    CAGCCCACAG ATGACTCCTG CTTCGACCCC TACACGGTCT CCCATTATGC CATTGGAGAG
    GAGTGGGAGC GATTGTCTGA TTCTGGCTTT AAACTCTCGT GCCAGTGCTT AGGCTTTGGC
    AGTGGTCATT TCAGATGCGA TTCATCTAAA TGGTGCCATG ACAATGGAGT GAACTACAAG
    ATTGGAGAGA AGTGGGATCG TCAGGGAGAG AATGGCCAGA TGATGAGCTG CACGTGTCTT
    GGAAATGGAA AAGGAGAATT CAAGTGTGAT CCTCATGAGG CCACGTGCTA TGACGATGGG
    AAGACTTACC ACGTGGGAGA ACAGTGGCAG AAGGAATATC TTGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA
    CCCGGTAACG AAGGCTCCAC TGCCCACTCC TACAACCAGT ATTCCCAGAG ATACCATCAG
    AGAACAAACA CTAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTGGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16668
    Clone ID Related Accession (Same CDS sequence) XM_005202775.3 , XM_005202775.4
    Accession Version XM_005202775.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG TTCAAAAGAC CCCTTTCATC ACCAACCCTG GGTATGACAC TGGAAACGGT
    ATTCAGCTTC CCGGCACTTC TGGTCAGCAG CCCAGTCTTG GGCAACAAAT GATCTTTGAG
    GAGCATGGTT TTAGGCGAAC CACACCGCCC ACCACGGCCA CCCCCGTAAG GCATAGGCCA
    AGACCGTATC CGCCGAATGT AAATGAGGAG ATCCAAATTG GTCATGTCCC CAGGGGAGAC
    GTAGACCATC ATCTCTACCC TCACGTTGTG GGACTCAATC CAAATGCTTC TACAGGCCAA
    GAAGCTCTCT CTCAGACAAC CATCTCATGG ACCCCATTCC AGGAAAGCTC TGAGTATATC
    ATTTCATGTC ATCCAGTTGG CATTGATGAA GAACCCTTAC AGTTCCGAGT TCCTGGAACC
    TCTGCTAGTG CCACCTTGAC GGGCCTCACC AGAGGGGCCA CCTACAACAT CATAGTGGAG
    GCAGTAAAAG ACCAGCAGAG GCAGAAGGTT CGCGAGGAGG TGGTTACCGT GGGCAATTCT
    GTTGACCAGG GCCTGAGTCA GCCCACAGAT GACTCCTGCT TCGACCCCTA CACGGTCTCC
    CATTATGCCA TTGGAGAGGA GTGGGAGCGA TTGTCTGATT CTGGCTTTAA ACTCTCGTGC
    CAGTGCTTAG GCTTTGGCAG TGGTCATTTC AGATGCGATT CATCTAAATG GTGCCATGAC
    AATGGAGTGA ACTACAAGAT TGGAGAGAAG TGGGATCGTC AGGGAGAGAA TGGCCAGATG
    ATGAGCTGCA CGTGTCTTGG AAATGGAAAA GGAGAATTCA AGTGTGATCC TCATGAGGCC
    ACGTGCTATG ACGATGGGAA GACTTACCAC GTGGGAGAAC AGTGGCAGAA GGAATATCTT
    GGTGCCATTT GCTCCTGCAC ATGCTTTGGA GGCCAGCGGG GCTGGCGCTG TGACAACTGC
    CGCAGACCTG GGGCTGAACC CGGTAACGAA GGCTCCACTG CCCACTCCTA CAACCAGTAT
    TCCCAGAGAT ACCATCAGAG AACAAACACT AATGTCAACT GCCCAATTGA GTGCTTCATG
    CCTTTGGATG TACAGGCTGA CAGAGAAGAT TCCCGAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202832.1
    CDS235..7596
    Translation

    Target ORF information:

    RefSeq Version XM_005202775.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202775.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG TTCAAAAGAC CCCTTTCATC ACCAACCCTG GGTATGACAC TGGAAACGGT
    ATTCAGCTTC CCGGCACTTC TGGTCAGCAG CCCAGTCTTG GGCAACAAAT GATCTTTGAG
    GAGCATGGTT TTAGGCGAAC CACACCGCCC ACCACGGCCA CCCCCGTAAG GCATAGGCCA
    AGACCGTATC CGCCGAATGT AAATGAGGAG ATCCAAATTG GTCATGTCCC CAGGGGAGAC
    GTAGACCATC ATCTCTACCC TCACGTTGTG GGACTCAATC CAAATGCTTC TACAGGCCAA
    GAAGCTCTCT CTCAGACAAC CATCTCATGG ACCCCATTCC AGGAAAGCTC TGAGTATATC
    ATTTCATGTC ATCCAGTTGG CATTGATGAA GAACCCTTAC AGTTCCGAGT TCCTGGAACC
    TCTGCTAGTG CCACCTTGAC GGGCCTCACC AGAGGGGCCA CCTACAACAT CATAGTGGAG
    GCAGTAAAAG ACCAGCAGAG GCAGAAGGTT CGCGAGGAGG TGGTTACCGT GGGCAATTCT
    GTTGACCAGG GCCTGAGTCA GCCCACAGAT GACTCCTGCT TCGACCCCTA CACGGTCTCC
    CATTATGCCA TTGGAGAGGA GTGGGAGCGA TTGTCTGATT CTGGCTTTAA ACTCTCGTGC
    CAGTGCTTAG GCTTTGGCAG TGGTCATTTC AGATGCGATT CATCTAAATG GTGCCATGAC
    AATGGAGTGA ACTACAAGAT TGGAGAGAAG TGGGATCGTC AGGGAGAGAA TGGCCAGATG
    ATGAGCTGCA CGTGTCTTGG AAATGGAAAA GGAGAATTCA AGTGTGATCC TCATGAGGCC
    ACGTGCTATG ACGATGGGAA GACTTACCAC GTGGGAGAAC AGTGGCAGAA GGAATATCTT
    GGTGCCATTT GCTCCTGCAC ATGCTTTGGA GGCCAGCGGG GCTGGCGCTG TGACAACTGC
    CGCAGACCTG GGGCTGAACC CGGTAACGAA GGCTCCACTG CCCACTCCTA CAACCAGTAT
    TCCCAGAGAT ACCATCAGAG AACAAACACT AATGTCAACT GCCCAATTGA GTGCTTCATG
    CCTTTGGATG TACAGGCTGA CAGAGAAGAT TCCCGAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16668
    Clone ID Related Accession (Same CDS sequence) XM_005202775.3 , XM_005202775.4
    Accession Version XM_005202775.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202775.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG TTCAAAAGAC CCCTTTCATC ACCAACCCTG GGTATGACAC TGGAAACGGT
    ATTCAGCTTC CCGGCACTTC TGGTCAGCAG CCCAGTCTTG GGCAACAAAT GATCTTTGAG
    GAGCATGGTT TTAGGCGAAC CACACCGCCC ACCACGGCCA CCCCCGTAAG GCATAGGCCA
    AGACCGTATC CGCCGAATGT AAATGAGGAG ATCCAAATTG GTCATGTCCC CAGGGGAGAC
    GTAGACCATC ATCTCTACCC TCACGTTGTG GGACTCAATC CAAATGCTTC TACAGGCCAA
    GAAGCTCTCT CTCAGACAAC CATCTCATGG ACCCCATTCC AGGAAAGCTC TGAGTATATC
    ATTTCATGTC ATCCAGTTGG CATTGATGAA GAACCCTTAC AGTTCCGAGT TCCTGGAACC
    TCTGCTAGTG CCACCTTGAC GGGCCTCACC AGAGGGGCCA CCTACAACAT CATAGTGGAG
    GCAGTAAAAG ACCAGCAGAG GCAGAAGGTT CGCGAGGAGG TGGTTACCGT GGGCAATTCT
    GTTGACCAGG GCCTGAGTCA GCCCACAGAT GACTCCTGCT TCGACCCCTA CACGGTCTCC
    CATTATGCCA TTGGAGAGGA GTGGGAGCGA TTGTCTGATT CTGGCTTTAA ACTCTCGTGC
    CAGTGCTTAG GCTTTGGCAG TGGTCATTTC AGATGCGATT CATCTAAATG GTGCCATGAC
    AATGGAGTGA ACTACAAGAT TGGAGAGAAG TGGGATCGTC AGGGAGAGAA TGGCCAGATG
    ATGAGCTGCA CGTGTCTTGG AAATGGAAAA GGAGAATTCA AGTGTGATCC TCATGAGGCC
    ACGTGCTATG ACGATGGGAA GACTTACCAC GTGGGAGAAC AGTGGCAGAA GGAATATCTT
    GGTGCCATTT GCTCCTGCAC ATGCTTTGGA GGCCAGCGGG GCTGGCGCTG TGACAACTGC
    CGCAGACCTG GGGCTGAACC CGGTAACGAA GGCTCCACTG CCCACTCCTA CAACCAGTAT
    TCCCAGAGAT ACCATCAGAG AACAAACACT AATGTCAACT GCCCAATTGA GTGCTTCATG
    CCTTTGGATG TACAGGCTGA CAGAGAAGAT TCCCGAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202832.1
    CDS239..7600
    Translation

    Target ORF information:

    RefSeq Version XM_005202775.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202775.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG TTCAAAAGAC CCCTTTCATC ACCAACCCTG GGTATGACAC TGGAAACGGT
    ATTCAGCTTC CCGGCACTTC TGGTCAGCAG CCCAGTCTTG GGCAACAAAT GATCTTTGAG
    GAGCATGGTT TTAGGCGAAC CACACCGCCC ACCACGGCCA CCCCCGTAAG GCATAGGCCA
    AGACCGTATC CGCCGAATGT AAATGAGGAG ATCCAAATTG GTCATGTCCC CAGGGGAGAC
    GTAGACCATC ATCTCTACCC TCACGTTGTG GGACTCAATC CAAATGCTTC TACAGGCCAA
    GAAGCTCTCT CTCAGACAAC CATCTCATGG ACCCCATTCC AGGAAAGCTC TGAGTATATC
    ATTTCATGTC ATCCAGTTGG CATTGATGAA GAACCCTTAC AGTTCCGAGT TCCTGGAACC
    TCTGCTAGTG CCACCTTGAC GGGCCTCACC AGAGGGGCCA CCTACAACAT CATAGTGGAG
    GCAGTAAAAG ACCAGCAGAG GCAGAAGGTT CGCGAGGAGG TGGTTACCGT GGGCAATTCT
    GTTGACCAGG GCCTGAGTCA GCCCACAGAT GACTCCTGCT TCGACCCCTA CACGGTCTCC
    CATTATGCCA TTGGAGAGGA GTGGGAGCGA TTGTCTGATT CTGGCTTTAA ACTCTCGTGC
    CAGTGCTTAG GCTTTGGCAG TGGTCATTTC AGATGCGATT CATCTAAATG GTGCCATGAC
    AATGGAGTGA ACTACAAGAT TGGAGAGAAG TGGGATCGTC AGGGAGAGAA TGGCCAGATG
    ATGAGCTGCA CGTGTCTTGG AAATGGAAAA GGAGAATTCA AGTGTGATCC TCATGAGGCC
    ACGTGCTATG ACGATGGGAA GACTTACCAC GTGGGAGAAC AGTGGCAGAA GGAATATCTT
    GGTGCCATTT GCTCCTGCAC ATGCTTTGGA GGCCAGCGGG GCTGGCGCTG TGACAACTGC
    CGCAGACCTG GGGCTGAACC CGGTAACGAA GGCTCCACTG CCCACTCCTA CAACCAGTAT
    TCCCAGAGAT ACCATCAGAG AACAAACACT AATGTCAACT GCCCAATTGA GTGCTTCATG
    CCTTTGGATG TACAGGCTGA CAGAGAAGAT TCCCGAGAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16669
    Clone ID Related Accession (Same CDS sequence) XM_005202776.3 , XM_005202776.4
    Accession Version XM_005202776.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG ATGAGCTCCC CCAGCTGGTA
    ACCCTTCCAC ACCCCAATCT TCATGGACCA GAGATCTTGG ATGTTCCCTC CACAGTTCAA
    AAGACCCCTT TCATCACCAA CCCTGGGTAT GACACTGGAA ACGGTATTCA GCTTCCCGGC
    ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA CAAATGATCT TTGAGGAGCA TGGTTTTAGG
    CGAACCACAC CGCCCACCAC GGCCACCCCC GTAAGGCATA GGCCAAGACC GTATCCGCCG
    AATGTAAATG AGGAGATCCA AATTGGTCAT GTCCCCAGGG GAGACGTAGA CCATCATCTC
    TACCCTCACG TTGTGGGACT CAATCCAAAT GCTTCTACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202833.1
    CDS235..7401
    Translation

    Target ORF information:

    RefSeq Version XM_005202776.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202776.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG ATGAGCTCCC CCAGCTGGTA
    ACCCTTCCAC ACCCCAATCT TCATGGACCA GAGATCTTGG ATGTTCCCTC CACAGTTCAA
    AAGACCCCTT TCATCACCAA CCCTGGGTAT GACACTGGAA ACGGTATTCA GCTTCCCGGC
    ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA CAAATGATCT TTGAGGAGCA TGGTTTTAGG
    CGAACCACAC CGCCCACCAC GGCCACCCCC GTAAGGCATA GGCCAAGACC GTATCCGCCG
    AATGTAAATG AGGAGATCCA AATTGGTCAT GTCCCCAGGG GAGACGTAGA CCATCATCTC
    TACCCTCACG TTGTGGGACT CAATCCAAAT GCTTCTACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16669
    Clone ID Related Accession (Same CDS sequence) XM_005202776.3 , XM_005202776.4
    Accession Version XM_005202776.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202776.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG ATGAGCTCCC CCAGCTGGTA
    ACCCTTCCAC ACCCCAATCT TCATGGACCA GAGATCTTGG ATGTTCCCTC CACAGTTCAA
    AAGACCCCTT TCATCACCAA CCCTGGGTAT GACACTGGAA ACGGTATTCA GCTTCCCGGC
    ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA CAAATGATCT TTGAGGAGCA TGGTTTTAGG
    CGAACCACAC CGCCCACCAC GGCCACCCCC GTAAGGCATA GGCCAAGACC GTATCCGCCG
    AATGTAAATG AGGAGATCCA AATTGGTCAT GTCCCCAGGG GAGACGTAGA CCATCATCTC
    TACCCTCACG TTGTGGGACT CAATCCAAAT GCTTCTACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202833.1
    CDS237..7403
    Translation

    Target ORF information:

    RefSeq Version XM_005202776.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202776.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG ATGAGCTCCC CCAGCTGGTA
    ACCCTTCCAC ACCCCAATCT TCATGGACCA GAGATCTTGG ATGTTCCCTC CACAGTTCAA
    AAGACCCCTT TCATCACCAA CCCTGGGTAT GACACTGGAA ACGGTATTCA GCTTCCCGGC
    ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA CAAATGATCT TTGAGGAGCA TGGTTTTAGG
    CGAACCACAC CGCCCACCAC GGCCACCCCC GTAAGGCATA GGCCAAGACC GTATCCGCCG
    AATGTAAATG AGGAGATCCA AATTGGTCAT GTCCCCAGGG GAGACGTAGA CCATCATCTC
    TACCCTCACG TTGTGGGACT CAATCCAAAT GCTTCTACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16670
    Clone ID Related Accession (Same CDS sequence) XM_005202777.4 , XM_005202777.3
    Accession Version XM_005202777.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202777.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG ATGAGCTCCC CCAGCTGGTA ACCCTTCCAC ACCCCAATCT TCATGGACCA
    GAGATCTTGG ATGTTCCCTC CACAGTTCAA AAGACCCCTT TCATCACCAA CCCTGGGTAT
    GACACTGGAA ACGGTATTCA GCTTCCCGGC ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA
    CAAATGATCT TTGAGGAGCA TGGTTTTAGG CGAACCACAC CGCCCACCAC GGCCACCCCC
    GTAAGGCATA GGCCAAGACC GTATCCGCCG AATGTAAATG AGGAGATCCA AATTGGTCAT
    GTCCCCAGGG GAGACGTAGA CCATCATCTC TACCCTCACG TTGTGGGACT CAATCCAAAT
    GCTTCTACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202834.1
    CDS238..7674
    Translation

    Target ORF information:

    RefSeq Version XM_005202777.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202777.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG ATGAGCTCCC CCAGCTGGTA ACCCTTCCAC ACCCCAATCT TCATGGACCA
    GAGATCTTGG ATGTTCCCTC CACAGTTCAA AAGACCCCTT TCATCACCAA CCCTGGGTAT
    GACACTGGAA ACGGTATTCA GCTTCCCGGC ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA
    CAAATGATCT TTGAGGAGCA TGGTTTTAGG CGAACCACAC CGCCCACCAC GGCCACCCCC
    GTAAGGCATA GGCCAAGACC GTATCCGCCG AATGTAAATG AGGAGATCCA AATTGGTCAT
    GTCCCCAGGG GAGACGTAGA CCATCATCTC TACCCTCACG TTGTGGGACT CAATCCAAAT
    GCTTCTACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16670
    Clone ID Related Accession (Same CDS sequence) XM_005202777.4 , XM_005202777.3
    Accession Version XM_005202777.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG ATGAGCTCCC CCAGCTGGTA ACCCTTCCAC ACCCCAATCT TCATGGACCA
    GAGATCTTGG ATGTTCCCTC CACAGTTCAA AAGACCCCTT TCATCACCAA CCCTGGGTAT
    GACACTGGAA ACGGTATTCA GCTTCCCGGC ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA
    CAAATGATCT TTGAGGAGCA TGGTTTTAGG CGAACCACAC CGCCCACCAC GGCCACCCCC
    GTAAGGCATA GGCCAAGACC GTATCCGCCG AATGTAAATG AGGAGATCCA AATTGGTCAT
    GTCCCCAGGG GAGACGTAGA CCATCATCTC TACCCTCACG TTGTGGGACT CAATCCAAAT
    GCTTCTACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202834.1
    CDS235..7671
    Translation

    Target ORF information:

    RefSeq Version XM_005202777.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202777.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG ATGAGCTCCC CCAGCTGGTA ACCCTTCCAC ACCCCAATCT TCATGGACCA
    GAGATCTTGG ATGTTCCCTC CACAGTTCAA AAGACCCCTT TCATCACCAA CCCTGGGTAT
    GACACTGGAA ACGGTATTCA GCTTCCCGGC ACTTCTGGTC AGCAGCCCAG TCTTGGGCAA
    CAAATGATCT TTGAGGAGCA TGGTTTTAGG CGAACCACAC CGCCCACCAC GGCCACCCCC
    GTAAGGCATA GGCCAAGACC GTATCCGCCG AATGTAAATG AGGAGATCCA AATTGGTCAT
    GTCCCCAGGG GAGACGTAGA CCATCATCTC TACCCTCACG TTGTGGGACT CAATCCAAAT
    GCTTCTACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16671
    Clone ID Related Accession (Same CDS sequence) XM_005202778.3 , XM_005202778.4
    Accession Version XM_005202778.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGTTC AAAAGACCCC TTTCATCACC
    AACCCTGGGT ATGACACTGG AAACGGTATT CAGCTTCCCG GCACTTCTGG TCAGCAGCCC
    AGTCTTGGGC AACAAATGAT CTTTGAGGAG CATGGTTTTA GGCGAACCAC ACCGCCCACC
    ACGGCCACCC CCGTAAGGCA TAGGCCAAGA CCGTATCCGC CGAATGTAAA TGAGGAGATC
    CAAATTGGTC ATGTCCCCAG GGGAGACGTA GACCATCATC TCTACCCTCA CGTTGTGGGA
    CTCAATCCAA ATGCTTCTAC AGGCCAAGAA GCTCTCTCTC AGACAACCAT CTCATGGACC
    CCATTCCAGG AAAGCTCTGA GTATATCATT TCATGTCATC CAGTTGGCAT TGATGAAGAA
    CCCTTACAGT TCCGAGTTCC TGGAACCTCT GCTAGTGCCA CCTTGACGGG CCTCACCAGA
    GGGGCCACCT ACAACATCAT AGTGGAGGCA GTAAAAGACC AGCAGAGGCA GAAGGTTCGC
    GAGGAGGTGG TTACCGTGGG CAATTCTGTT GACCAGGGCC TGAGTCAGCC CACAGATGAC
    TCCTGCTTCG ACCCCTACAC GGTCTCCCAT TATGCCATTG GAGAGGAGTG GGAGCGATTG
    TCTGATTCTG GCTTTAAACT CTCGTGCCAG TGCTTAGGCT TTGGCAGTGG TCATTTCAGA
    TGCGATTCAT CTAAATGGTG CCATGACAAT GGAGTGAACT ACAAGATTGG AGAGAAGTGG
    GATCGTCAGG GAGAGAATGG CCAGATGATG AGCTGCACGT GTCTTGGAAA TGGAAAAGGA
    GAATTCAAGT GTGATCCTCA TGAGGCCACG TGCTATGACG ATGGGAAGAC TTACCACGTG
    GGAGAACAGT GGCAGAAGGA ATATCTTGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG CTGAACCCGG TAACGAAGGC
    TCCACTGCCC ACTCCTACAA CCAGTATTCC CAGAGATACC ATCAGAGAAC AAACACTAAT
    GTCAACTGCC CAATTGAGTG CTTCATGCCT TTGGATGTAC AGGCTGACAG AGAAGATTCC
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202835.1
    CDS235..7323
    Translation

    Target ORF information:

    RefSeq Version XM_005202778.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202778.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGTTC AAAAGACCCC TTTCATCACC
    AACCCTGGGT ATGACACTGG AAACGGTATT CAGCTTCCCG GCACTTCTGG TCAGCAGCCC
    AGTCTTGGGC AACAAATGAT CTTTGAGGAG CATGGTTTTA GGCGAACCAC ACCGCCCACC
    ACGGCCACCC CCGTAAGGCA TAGGCCAAGA CCGTATCCGC CGAATGTAAA TGAGGAGATC
    CAAATTGGTC ATGTCCCCAG GGGAGACGTA GACCATCATC TCTACCCTCA CGTTGTGGGA
    CTCAATCCAA ATGCTTCTAC AGGCCAAGAA GCTCTCTCTC AGACAACCAT CTCATGGACC
    CCATTCCAGG AAAGCTCTGA GTATATCATT TCATGTCATC CAGTTGGCAT TGATGAAGAA
    CCCTTACAGT TCCGAGTTCC TGGAACCTCT GCTAGTGCCA CCTTGACGGG CCTCACCAGA
    GGGGCCACCT ACAACATCAT AGTGGAGGCA GTAAAAGACC AGCAGAGGCA GAAGGTTCGC
    GAGGAGGTGG TTACCGTGGG CAATTCTGTT GACCAGGGCC TGAGTCAGCC CACAGATGAC
    TCCTGCTTCG ACCCCTACAC GGTCTCCCAT TATGCCATTG GAGAGGAGTG GGAGCGATTG
    TCTGATTCTG GCTTTAAACT CTCGTGCCAG TGCTTAGGCT TTGGCAGTGG TCATTTCAGA
    TGCGATTCAT CTAAATGGTG CCATGACAAT GGAGTGAACT ACAAGATTGG AGAGAAGTGG
    GATCGTCAGG GAGAGAATGG CCAGATGATG AGCTGCACGT GTCTTGGAAA TGGAAAAGGA
    GAATTCAAGT GTGATCCTCA TGAGGCCACG TGCTATGACG ATGGGAAGAC TTACCACGTG
    GGAGAACAGT GGCAGAAGGA ATATCTTGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG CTGAACCCGG TAACGAAGGC
    TCCACTGCCC ACTCCTACAA CCAGTATTCC CAGAGATACC ATCAGAGAAC AAACACTAAT
    GTCAACTGCC CAATTGAGTG CTTCATGCCT TTGGATGTAC AGGCTGACAG AGAAGATTCC
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16671
    Clone ID Related Accession (Same CDS sequence) XM_005202778.3 , XM_005202778.4
    Accession Version XM_005202778.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202778.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGTTC AAAAGACCCC TTTCATCACC
    AACCCTGGGT ATGACACTGG AAACGGTATT CAGCTTCCCG GCACTTCTGG TCAGCAGCCC
    AGTCTTGGGC AACAAATGAT CTTTGAGGAG CATGGTTTTA GGCGAACCAC ACCGCCCACC
    ACGGCCACCC CCGTAAGGCA TAGGCCAAGA CCGTATCCGC CGAATGTAAA TGAGGAGATC
    CAAATTGGTC ATGTCCCCAG GGGAGACGTA GACCATCATC TCTACCCTCA CGTTGTGGGA
    CTCAATCCAA ATGCTTCTAC AGGCCAAGAA GCTCTCTCTC AGACAACCAT CTCATGGACC
    CCATTCCAGG AAAGCTCTGA GTATATCATT TCATGTCATC CAGTTGGCAT TGATGAAGAA
    CCCTTACAGT TCCGAGTTCC TGGAACCTCT GCTAGTGCCA CCTTGACGGG CCTCACCAGA
    GGGGCCACCT ACAACATCAT AGTGGAGGCA GTAAAAGACC AGCAGAGGCA GAAGGTTCGC
    GAGGAGGTGG TTACCGTGGG CAATTCTGTT GACCAGGGCC TGAGTCAGCC CACAGATGAC
    TCCTGCTTCG ACCCCTACAC GGTCTCCCAT TATGCCATTG GAGAGGAGTG GGAGCGATTG
    TCTGATTCTG GCTTTAAACT CTCGTGCCAG TGCTTAGGCT TTGGCAGTGG TCATTTCAGA
    TGCGATTCAT CTAAATGGTG CCATGACAAT GGAGTGAACT ACAAGATTGG AGAGAAGTGG
    GATCGTCAGG GAGAGAATGG CCAGATGATG AGCTGCACGT GTCTTGGAAA TGGAAAAGGA
    GAATTCAAGT GTGATCCTCA TGAGGCCACG TGCTATGACG ATGGGAAGAC TTACCACGTG
    GGAGAACAGT GGCAGAAGGA ATATCTTGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG CTGAACCCGG TAACGAAGGC
    TCCACTGCCC ACTCCTACAA CCAGTATTCC CAGAGATACC ATCAGAGAAC AAACACTAAT
    GTCAACTGCC CAATTGAGTG CTTCATGCCT TTGGATGTAC AGGCTGACAG AGAAGATTCC
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202835.1
    CDS238..7326
    Translation

    Target ORF information:

    RefSeq Version XM_005202778.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202778.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGTTC AAAAGACCCC TTTCATCACC
    AACCCTGGGT ATGACACTGG AAACGGTATT CAGCTTCCCG GCACTTCTGG TCAGCAGCCC
    AGTCTTGGGC AACAAATGAT CTTTGAGGAG CATGGTTTTA GGCGAACCAC ACCGCCCACC
    ACGGCCACCC CCGTAAGGCA TAGGCCAAGA CCGTATCCGC CGAATGTAAA TGAGGAGATC
    CAAATTGGTC ATGTCCCCAG GGGAGACGTA GACCATCATC TCTACCCTCA CGTTGTGGGA
    CTCAATCCAA ATGCTTCTAC AGGCCAAGAA GCTCTCTCTC AGACAACCAT CTCATGGACC
    CCATTCCAGG AAAGCTCTGA GTATATCATT TCATGTCATC CAGTTGGCAT TGATGAAGAA
    CCCTTACAGT TCCGAGTTCC TGGAACCTCT GCTAGTGCCA CCTTGACGGG CCTCACCAGA
    GGGGCCACCT ACAACATCAT AGTGGAGGCA GTAAAAGACC AGCAGAGGCA GAAGGTTCGC
    GAGGAGGTGG TTACCGTGGG CAATTCTGTT GACCAGGGCC TGAGTCAGCC CACAGATGAC
    TCCTGCTTCG ACCCCTACAC GGTCTCCCAT TATGCCATTG GAGAGGAGTG GGAGCGATTG
    TCTGATTCTG GCTTTAAACT CTCGTGCCAG TGCTTAGGCT TTGGCAGTGG TCATTTCAGA
    TGCGATTCAT CTAAATGGTG CCATGACAAT GGAGTGAACT ACAAGATTGG AGAGAAGTGG
    GATCGTCAGG GAGAGAATGG CCAGATGATG AGCTGCACGT GTCTTGGAAA TGGAAAAGGA
    GAATTCAAGT GTGATCCTCA TGAGGCCACG TGCTATGACG ATGGGAAGAC TTACCACGTG
    GGAGAACAGT GGCAGAAGGA ATATCTTGGT GCCATTTGCT CCTGCACATG CTTTGGAGGC
    CAGCGGGGCT GGCGCTGTGA CAACTGCCGC AGACCTGGGG CTGAACCCGG TAACGAAGGC
    TCCACTGCCC ACTCCTACAA CCAGTATTCC CAGAGATACC ATCAGAGAAC AAACACTAAT
    GTCAACTGCC CAATTGAGTG CTTCATGCCT TTGGATGTAC AGGCTGACAG AGAAGATTCC
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16672
    Clone ID Related Accession (Same CDS sequence) XM_005202779.4 , XM_005202779.3
    Accession Version XM_005202779.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202779.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202836.1
    CDS238..7314
    Translation

    Target ORF information:

    RefSeq Version XM_005202779.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202779.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16672
    Clone ID Related Accession (Same CDS sequence) XM_005202779.4 , XM_005202779.3
    Accession Version XM_005202779.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202836.1
    CDS235..7311
    Translation

    Target ORF information:

    RefSeq Version XM_005202779.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202779.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCAACA TTGATCGCCC TAAAGGACTG GCATTCACTG ATGTGGATGT CGATTCCATC
    AAAATTGCTT GGGAAAGCCC ACAGGGGCAA GTTTCCAGGT ACAGGGTGAC CTACTCGAGC
    CCTGAGGATG GAATCCATGA GCTATTCCCT GCACCTGATG GTGAAGAAGA GACTGCAGAG
    CTGCAAGGCC TCAGGCCGGG TTCTGAGTAC ACAGTCAGTG TGGTTGCCTT GCACGATGAT
    ATGGAGAGCC AGCCCCTGAT TGGAACCCAG TCCACAGCCA TTCCTGCACC AACCAACCTG
    AAGTTTACTC AGGTGACACC AACCAGCCTG ACTGCCCAGT GGACGGCACC CAATGTTCAG
    CTCACTGGGT ATCGAGTGCG GGTGACCCCG AAGGAGAAGA CCGGACCGAT GAAAGAAATC
    AACCTTGCTC CTGATAGCTC ATCCGTGGTT GTATCAGGAC TCATGGTTGC CACCAAATAT
    GAAGTGAGTG TCTATGCTCT TAAGGACACT TTGACAAGCA GACCGGCTCA GGGAGTCGTT
    ACAACTCTGG AGAATGTCAG CCCTCCAAGA AGGGCCCGTG TGACAGATGC TACTGAGACC
    ACCATCACCA TTAGCTGGAG AACTAAGACT GAGACGATCA CCGGCTTCCA AGTTGATGCT
    ATCCCAGCAA ATGGCCAGAC CCCAATTCAG AGAACCATCA GGCCAGATGT CAGAAGTTAC
    ACCATCACAG GTTTACAACC TGGTACTGAC TACAAGATCC ACTTGTACAC CTTGAATGAC
    AATGCCCGGA GCTCCCCTGT GGTCATCGAT GCCTCCACTG CCATTGATGC ACCATCCAAC
    CTGCGTTTCC TGGCCACCAC ACCCAACTCC CTGCTGGTAT CATGGCAGCC ACCCCGTGCC
    AGGATTACTG GTTACATCAT CAAGTACGAG AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC
    CCTCGGCCCC GCCCTGGTGT CACAGAAGCT ACTATCACTG GTCTGGAACC AGGAACAGAG
    TACACAATCC AAGTCATTGC CCTCAAGAAC AATCAGAAGA GTGAGCCTTT GATTGGGAGG
    AAAAAGACAG GCCAAGAAGC TCTCTCTCAG ACAACCATCT CATGGACCCC ATTCCAGGAA
    AGCTCTGAGT ATATCATTTC ATGTCATCCA GTTGGCATTG ATGAAGAACC CTTACAGTTC
    CGAGTTCCTG GAACCTCTGC TAGTGCCACC TTGACGGGCC TCACCAGAGG GGCCACCTAC
    AACATCATAG TGGAGGCAGT AAAAGACCAG CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT
    ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG AGTCAGCCCA CAGATGACTC CTGCTTCGAC
    CCCTACACGG TCTCCCATTA TGCCATTGGA GAGGAGTGGG AGCGATTGTC TGATTCTGGC
    TTTAAACTCT CGTGCCAGTG CTTAGGCTTT GGCAGTGGTC ATTTCAGATG CGATTCATCT
    AAATGGTGCC ATGACAATGG AGTGAACTAC AAGATTGGAG AGAAGTGGGA TCGTCAGGGA
    GAGAATGGCC AGATGATGAG CTGCACGTGT CTTGGAAATG GAAAAGGAGA ATTCAAGTGT
    GATCCTCATG AGGCCACGTG CTATGACGAT GGGAAGACTT ACCACGTGGG AGAACAGTGG
    CAGAAGGAAT ATCTTGGTGC CATTTGCTCC TGCACATGCT TTGGAGGCCA GCGGGGCTGG
    CGCTGTGACA ACTGCCGCAG ACCTGGGGCT GAACCCGGTA ACGAAGGCTC CACTGCCCAC
    TCCTACAACC AGTATTCCCA GAGATACCAT CAGAGAACAA ACACTAATGT CAACTGCCCA
    ATTGAGTGCT TCATGCCTTT GGATGTACAG GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16675
    Clone ID Related Accession (Same CDS sequence) XM_005202782.3 , XM_005202782.4
    Accession Version XM_005202782.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGATG AGCTCCCCCA GCTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC
    ACTGGAAACG GTATTCAGCT TCCCGGCACT TCTGGTCAGC AGCCCAGTCT TGGGCAACAA
    ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACCACGGC CACCCCCGTA
    AGGCATAGGC CAAGACCGTA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC
    CCCAGGGGAG ACGTAGACCA TCATCTCTAC CCTCACGTTG TGGGACTCAA TCCAAATGCT
    TCTACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202839.1
    CDS234..7127
    Translation

    Target ORF information:

    RefSeq Version XM_005202782.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202782.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGATG AGCTCCCCCA GCTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC
    ACTGGAAACG GTATTCAGCT TCCCGGCACT TCTGGTCAGC AGCCCAGTCT TGGGCAACAA
    ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACCACGGC CACCCCCGTA
    AGGCATAGGC CAAGACCGTA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC
    CCCAGGGGAG ACGTAGACCA TCATCTCTAC CCTCACGTTG TGGGACTCAA TCCAAATGCT
    TCTACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16675
    Clone ID Related Accession (Same CDS sequence) XM_005202782.3 , XM_005202782.4
    Accession Version XM_005202782.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202782.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGATG AGCTCCCCCA GCTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC
    ACTGGAAACG GTATTCAGCT TCCCGGCACT TCTGGTCAGC AGCCCAGTCT TGGGCAACAA
    ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACCACGGC CACCCCCGTA
    AGGCATAGGC CAAGACCGTA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC
    CCCAGGGGAG ACGTAGACCA TCATCTCTAC CCTCACGTTG TGGGACTCAA TCCAAATGCT
    TCTACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202839.1
    CDS357..7250
    Translation

    Target ORF information:

    RefSeq Version XM_005202782.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202782.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGATG AGCTCCCCCA GCTGGTAACC CTTCCACACC CCAATCTTCA TGGACCAGAG
    ATCTTGGATG TTCCCTCCAC AGTTCAAAAG ACCCCTTTCA TCACCAACCC TGGGTATGAC
    ACTGGAAACG GTATTCAGCT TCCCGGCACT TCTGGTCAGC AGCCCAGTCT TGGGCAACAA
    ATGATCTTTG AGGAGCATGG TTTTAGGCGA ACCACACCGC CCACCACGGC CACCCCCGTA
    AGGCATAGGC CAAGACCGTA TCCGCCGAAT GTAAATGAGG AGATCCAAAT TGGTCATGTC
    CCCAGGGGAG ACGTAGACCA TCATCTCTAC CCTCACGTTG TGGGACTCAA TCCAAATGCT
    TCTACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16676
    Clone ID Related Accession (Same CDS sequence) XM_005202783.3 , XM_005202783.4
    Accession Version XM_005202783.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCATCACC AACCCTGGGT ATGACACTGG AAACGGTATT
    CAGCTTCCCG GCACTTCTGG TCAGCAGCCC AGTCTTGGGC AACAAATGAT CTTTGAGGAG
    CATGGTTTTA GGCGAACCAC ACCGCCCACC ACGGCCACCC CCGTAAGGCA TAGGCCAAGA
    CCGTATCCGC CGAATGTAAA TGAGGAGATC CAAATTGGTC ATGTCCCCAG GGGAGACGTA
    GACCATCATC TCTACCCTCA CGTTGTGGGA CTCAATCCAA ATGCTTCTAC AGGCCAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGACC CCATTCCAGG AAAGCTCTGA GTATATCATT
    TCATGTCATC CAGTTGGCAT TGATGAAGAA CCCTTACAGT TCCGAGTTCC TGGAACCTCT
    GCTAGTGCCA CCTTGACGGG CCTCACCAGA GGGGCCACCT ACAACATCAT AGTGGAGGCA
    GTAAAAGACC AGCAGAGGCA GAAGGTTCGC GAGGAGGTGG TTACCGTGGG CAATTCTGTT
    GACCAGGGCC TGAGTCAGCC CACAGATGAC TCCTGCTTCG ACCCCTACAC GGTCTCCCAT
    TATGCCATTG GAGAGGAGTG GGAGCGATTG TCTGATTCTG GCTTTAAACT CTCGTGCCAG
    TGCTTAGGCT TTGGCAGTGG TCATTTCAGA TGCGATTCAT CTAAATGGTG CCATGACAAT
    GGAGTGAACT ACAAGATTGG AGAGAAGTGG GATCGTCAGG GAGAGAATGG CCAGATGATG
    AGCTGCACGT GTCTTGGAAA TGGAAAAGGA GAATTCAAGT GTGATCCTCA TGAGGCCACG
    TGCTATGACG ATGGGAAGAC TTACCACGTG GGAGAACAGT GGCAGAAGGA ATATCTTGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG CTGAACCCGG TAACGAAGGC TCCACTGCCC ACTCCTACAA CCAGTATTCC
    CAGAGATACC ATCAGAGAAC AAACACTAAT GTCAACTGCC CAATTGAGTG CTTCATGCCT
    TTGGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202840.1
    CDS234..7052
    Translation

    Target ORF information:

    RefSeq Version XM_005202783.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202783.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCATCACC AACCCTGGGT ATGACACTGG AAACGGTATT
    CAGCTTCCCG GCACTTCTGG TCAGCAGCCC AGTCTTGGGC AACAAATGAT CTTTGAGGAG
    CATGGTTTTA GGCGAACCAC ACCGCCCACC ACGGCCACCC CCGTAAGGCA TAGGCCAAGA
    CCGTATCCGC CGAATGTAAA TGAGGAGATC CAAATTGGTC ATGTCCCCAG GGGAGACGTA
    GACCATCATC TCTACCCTCA CGTTGTGGGA CTCAATCCAA ATGCTTCTAC AGGCCAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGACC CCATTCCAGG AAAGCTCTGA GTATATCATT
    TCATGTCATC CAGTTGGCAT TGATGAAGAA CCCTTACAGT TCCGAGTTCC TGGAACCTCT
    GCTAGTGCCA CCTTGACGGG CCTCACCAGA GGGGCCACCT ACAACATCAT AGTGGAGGCA
    GTAAAAGACC AGCAGAGGCA GAAGGTTCGC GAGGAGGTGG TTACCGTGGG CAATTCTGTT
    GACCAGGGCC TGAGTCAGCC CACAGATGAC TCCTGCTTCG ACCCCTACAC GGTCTCCCAT
    TATGCCATTG GAGAGGAGTG GGAGCGATTG TCTGATTCTG GCTTTAAACT CTCGTGCCAG
    TGCTTAGGCT TTGGCAGTGG TCATTTCAGA TGCGATTCAT CTAAATGGTG CCATGACAAT
    GGAGTGAACT ACAAGATTGG AGAGAAGTGG GATCGTCAGG GAGAGAATGG CCAGATGATG
    AGCTGCACGT GTCTTGGAAA TGGAAAAGGA GAATTCAAGT GTGATCCTCA TGAGGCCACG
    TGCTATGACG ATGGGAAGAC TTACCACGTG GGAGAACAGT GGCAGAAGGA ATATCTTGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG CTGAACCCGG TAACGAAGGC TCCACTGCCC ACTCCTACAA CCAGTATTCC
    CAGAGATACC ATCAGAGAAC AAACACTAAT GTCAACTGCC CAATTGAGTG CTTCATGCCT
    TTGGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16676
    Clone ID Related Accession (Same CDS sequence) XM_005202783.3 , XM_005202783.4
    Accession Version XM_005202783.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202783.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCATCACC AACCCTGGGT ATGACACTGG AAACGGTATT
    CAGCTTCCCG GCACTTCTGG TCAGCAGCCC AGTCTTGGGC AACAAATGAT CTTTGAGGAG
    CATGGTTTTA GGCGAACCAC ACCGCCCACC ACGGCCACCC CCGTAAGGCA TAGGCCAAGA
    CCGTATCCGC CGAATGTAAA TGAGGAGATC CAAATTGGTC ATGTCCCCAG GGGAGACGTA
    GACCATCATC TCTACCCTCA CGTTGTGGGA CTCAATCCAA ATGCTTCTAC AGGCCAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGACC CCATTCCAGG AAAGCTCTGA GTATATCATT
    TCATGTCATC CAGTTGGCAT TGATGAAGAA CCCTTACAGT TCCGAGTTCC TGGAACCTCT
    GCTAGTGCCA CCTTGACGGG CCTCACCAGA GGGGCCACCT ACAACATCAT AGTGGAGGCA
    GTAAAAGACC AGCAGAGGCA GAAGGTTCGC GAGGAGGTGG TTACCGTGGG CAATTCTGTT
    GACCAGGGCC TGAGTCAGCC CACAGATGAC TCCTGCTTCG ACCCCTACAC GGTCTCCCAT
    TATGCCATTG GAGAGGAGTG GGAGCGATTG TCTGATTCTG GCTTTAAACT CTCGTGCCAG
    TGCTTAGGCT TTGGCAGTGG TCATTTCAGA TGCGATTCAT CTAAATGGTG CCATGACAAT
    GGAGTGAACT ACAAGATTGG AGAGAAGTGG GATCGTCAGG GAGAGAATGG CCAGATGATG
    AGCTGCACGT GTCTTGGAAA TGGAAAAGGA GAATTCAAGT GTGATCCTCA TGAGGCCACG
    TGCTATGACG ATGGGAAGAC TTACCACGTG GGAGAACAGT GGCAGAAGGA ATATCTTGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG CTGAACCCGG TAACGAAGGC TCCACTGCCC ACTCCTACAA CCAGTATTCC
    CAGAGATACC ATCAGAGAAC AAACACTAAT GTCAACTGCC CAATTGAGTG CTTCATGCCT
    TTGGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202840.1
    CDS287..7105
    Translation

    Target ORF information:

    RefSeq Version XM_005202783.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202783.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGTTC AAAAGACCCC TTTCATCACC AACCCTGGGT ATGACACTGG AAACGGTATT
    CAGCTTCCCG GCACTTCTGG TCAGCAGCCC AGTCTTGGGC AACAAATGAT CTTTGAGGAG
    CATGGTTTTA GGCGAACCAC ACCGCCCACC ACGGCCACCC CCGTAAGGCA TAGGCCAAGA
    CCGTATCCGC CGAATGTAAA TGAGGAGATC CAAATTGGTC ATGTCCCCAG GGGAGACGTA
    GACCATCATC TCTACCCTCA CGTTGTGGGA CTCAATCCAA ATGCTTCTAC AGGCCAAGAA
    GCTCTCTCTC AGACAACCAT CTCATGGACC CCATTCCAGG AAAGCTCTGA GTATATCATT
    TCATGTCATC CAGTTGGCAT TGATGAAGAA CCCTTACAGT TCCGAGTTCC TGGAACCTCT
    GCTAGTGCCA CCTTGACGGG CCTCACCAGA GGGGCCACCT ACAACATCAT AGTGGAGGCA
    GTAAAAGACC AGCAGAGGCA GAAGGTTCGC GAGGAGGTGG TTACCGTGGG CAATTCTGTT
    GACCAGGGCC TGAGTCAGCC CACAGATGAC TCCTGCTTCG ACCCCTACAC GGTCTCCCAT
    TATGCCATTG GAGAGGAGTG GGAGCGATTG TCTGATTCTG GCTTTAAACT CTCGTGCCAG
    TGCTTAGGCT TTGGCAGTGG TCATTTCAGA TGCGATTCAT CTAAATGGTG CCATGACAAT
    GGAGTGAACT ACAAGATTGG AGAGAAGTGG GATCGTCAGG GAGAGAATGG CCAGATGATG
    AGCTGCACGT GTCTTGGAAA TGGAAAAGGA GAATTCAAGT GTGATCCTCA TGAGGCCACG
    TGCTATGACG ATGGGAAGAC TTACCACGTG GGAGAACAGT GGCAGAAGGA ATATCTTGGT
    GCCATTTGCT CCTGCACATG CTTTGGAGGC CAGCGGGGCT GGCGCTGTGA CAACTGCCGC
    AGACCTGGGG CTGAACCCGG TAACGAAGGC TCCACTGCCC ACTCCTACAA CCAGTATTCC
    CAGAGATACC ATCAGAGAAC AAACACTAAT GTCAACTGCC CAATTGAGTG CTTCATGCCT
    TTGGATGTAC AGGCTGACAG AGAAGATTCC CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16677
    Clone ID Related Accession (Same CDS sequence) XM_005202784.3 , XM_005202784.4
    Accession Version XM_005202784.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202841.1
    CDS235..7041
    Translation

    Target ORF information:

    RefSeq Version XM_005202784.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202784.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16677
    Clone ID Related Accession (Same CDS sequence) XM_005202784.3 , XM_005202784.4
    Accession Version XM_005202784.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202784.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202841.1
    CDS238..7044
    Translation

    Target ORF information:

    RefSeq Version XM_005202784.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202784.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGA GGTGCCCCAA CTCACTGACC TAAGCTTTGT TGATATAACT
    GATTCAAGCA TCGGCCTGAG GTGGACCCCG CTAAACTCTT CCACCATTAT TGGGTACCGC
    ATCACAGTAG TTGCGGCAGG AGAAGGTATC CCTATTTTTG AAGATTTTGT GGACTCCTCA
    GTAGGATACT ACACAGTCAC AGGGCTGGAG CCCGGCATTG ACTATGACAT CAGCGTTATC
    ACTCTCATTA ATGGCGGAGA GAGTGCCCCT ACTACACTGA CACAACAAAC GGCTGTCCCT
    CCTCCCACTG ATTTGCGATT TACCAATGTT GGTCCTGACA CAATGCGTGT CACCTGGGCT
    CCACCCTCAT CCATTGAACT GACCAACCTC CTGGTACGCT ACTCGCCTGT GAAAAATGAG
    GAGGATGTTG CCGAGCTGTC CATTTCTCCT TCAGACAACG CAGTGGTCTT AACAAATCTC
    CTGCCCGGCA CCGAGTATTT AGTCAGCGTC TCCAGTGTTT ACGAGCAGCA TGAGAGCATA
    CCTCTTAGAG GAAGACAGAA AACCGCTCTT GACTCCCCAA GTGGCATTGA CTTTTCTGAT
    ATCACTGCCA ACTCTTTCAC TGTCCATTGG ATTGCTCCAC GAGCCACCAT CACTGGCTAC
    AGGATCCGCC ATCATCCTGA AAACATGGGT GGAAGACCTC GGGAAGATCG AGTGCCCCCC
    TCTCGGAATT CCATCACCCT CACCAACCTG AACCCAGGCA CAGAATATGT GGTCAGCATT
    GTTGCTCTTA ATAGCAAAGA GGAAAGTCTG CCCTTGGTTG GCCAACAATC AACAGTTTCT
    GATGTTCCAA GGGACCTGGA AGTCATTGCT GCAACCCCCA CTAGCCTGCT GATCAGCTGG
    GATGCGCCTG CCGTCACAGT GAGATACTAC AGGATCACCT ATGGAGAAAC AGGAGGAAGT
    AGCCCTGTCC AGGAGTTCAC CGTGCCTGGG AGCAAGTCTA CAGCTACCAT CAGTGGTCTT
    AAACCTGGAG TGGACTACAC CATCACTGTC TATGCTGTCA CCGGCCGTGG GGACAGCCCG
    GCAAGCAGCA AGCCCGTTTC CATCAATTAC CGAACAGAAA TTGACAAACC ATCCCAGATG
    CAAGTGACTG ATGTCCAAGA CAACAGCATT AGTGTCAGGT GGCTGCCTTC AAGTTCCCCT
    GTTACTGGTT ACAGAGTGAC CACTGCTCCT AAAAATGGCC CAGGACCATC GAAAACGAAA
    ACTGTAGGTC CAGATCAAAC AGAAATGACA ATTGAAGGCT TGCAGCCCAC AGTGGAGTAT
    GTGGTCAGTG TCTATGCTCA GAATCAAAAC GGAGAGAGTC AGCCTCTGGT TCAGACAGCG
    GTAACCACCA TTCCTGCACC AACCAACCTG AAGTTTACTC AGGTGACACC AACCAGCCTG
    ACTGCCCAGT GGACGGCACC CAATGTTCAG CTCACTGGGT ATCGAGTGCG GGTGACCCCG
    AAGGAGAAGA CCGGACCGAT GAAAGAAATC AACCTTGCTC CTGATAGCTC ATCCGTGGTT
    GTATCAGGAC TCATGGTTGC CACCAAATAT GAAGTGAGTG TCTATGCTCT TAAGGACACT
    TTGACAAGCA GACCGGCTCA GGGAGTCGTT ACAACTCTGG AGAATGTCAG CCCTCCAAGA
    AGGGCCCGTG TGACAGATGC TACTGAGACC ACCATCACCA TTAGCTGGAG AACTAAGACT
    GAGACGATCA CCGGCTTCCA AGTTGATGCT ATCCCAGCAA ATGGCCAGAC CCCAATTCAG
    AGAACCATCA GGCCAGATGT CAGAAGTTAC ACCATCACAG GTTTACAACC TGGTACTGAC
    TACAAGATCC ACTTGTACAC CTTGAATGAC AATGCCCGGA GCTCCCCTGT GGTCATCGAT
    GCCTCCACTG CCATTGATGC ACCATCCAAC CTGCGTTTCC TGGCCACCAC ACCCAACTCC
    CTGCTGGTAT CATGGCAGCC ACCCCGTGCC AGGATTACTG GTTACATCAT CAAGTACGAG
    AAGCCTGGGT CCCCTCCTAG AGAAGTGGTC CCTCGGCCCC GCCCTGGTGT CACAGAAGCT
    ACTATCACTG GTCTGGAACC AGGAACAGAG TACACAATCC AAGTCATTGC CCTCAAGAAC
    AATCAGAAGA GTGAGCCTTT GATTGGGAGG AAAAAGACAG GCCAAGAAGC TCTCTCTCAG
    ACAACCATCT CATGGACCCC ATTCCAGGAA AGCTCTGAGT ATATCATTTC ATGTCATCCA
    GTTGGCATTG ATGAAGAACC CTTACAGTTC CGAGTTCCTG GAACCTCTGC TAGTGCCACC
    TTGACGGGCC TCACCAGAGG GGCCACCTAC AACATCATAG TGGAGGCAGT AAAAGACCAG
    CAGAGGCAGA AGGTTCGCGA GGAGGTGGTT ACCGTGGGCA ATTCTGTTGA CCAGGGCCTG
    AGTCAGCCCA CAGATGACTC CTGCTTCGAC CCCTACACGG TCTCCCATTA TGCCATTGGA
    GAGGAGTGGG AGCGATTGTC TGATTCTGGC TTTAAACTCT CGTGCCAGTG CTTAGGCTTT
    GGCAGTGGTC ATTTCAGATG CGATTCATCT AAATGGTGCC ATGACAATGG AGTGAACTAC
    AAGATTGGAG AGAAGTGGGA TCGTCAGGGA GAGAATGGCC AGATGATGAG CTGCACGTGT
    CTTGGAAATG GAAAAGGAGA ATTCAAGTGT GATCCTCATG AGGCCACGTG CTATGACGAT
    GGGAAGACTT ACCACGTGGG AGAACAGTGG CAGAAGGAAT ATCTTGGTGC CATTTGCTCC
    TGCACATGCT TTGGAGGCCA GCGGGGCTGG CGCTGTGACA ACTGCCGCAG ACCTGGGGCT
    GAACCCGGTA ACGAAGGCTC CACTGCCCAC TCCTACAACC AGTATTCCCA GAGATACCAT
    CAGAGAACAA ACACTAATGT CAACTGCCCA ATTGAGTGCT TCATGCCTTT GGATGTACAG
    GCTGACAGAG AAGATTCCCG AGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16678
    Clone ID Related Accession (Same CDS sequence) XM_005202785.3 , XM_005202785.4
    Accession Version XM_005202785.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202785.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGGCC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT
    GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCTTG
    ACGGGCCTCA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCAGTAAA AGACCAGCAG
    AGGCAGAAGG TTCGCGAGGA GGTGGTTACC GTGGGCAATT CTGTTGACCA GGGCCTGAGT
    CAGCCCACAG ATGACTCCTG CTTCGACCCC TACACGGTCT CCCATTATGC CATTGGAGAG
    GAGTGGGAGC GATTGTCTGA TTCTGGCTTT AAACTCTCGT GCCAGTGCTT AGGCTTTGGC
    AGTGGTCATT TCAGATGCGA TTCATCTAAA TGGTGCCATG ACAATGGAGT GAACTACAAG
    ATTGGAGAGA AGTGGGATCG TCAGGGAGAG AATGGCCAGA TGATGAGCTG CACGTGTCTT
    GGAAATGGAA AAGGAGAATT CAAGTGTGAT CCTCATGAGG CCACGTGCTA TGACGATGGG
    AAGACTTACC ACGTGGGAGA ACAGTGGCAG AAGGAATATC TTGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA
    CCCGGTAACG AAGGCTCCAC TGCCCACTCC TACAACCAGT ATTCCCAGAG ATACCATCAG
    AGAACAAACA CTAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTGGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202842.1
    CDS235..7038
    Translation

    Target ORF information:

    RefSeq Version XM_005202785.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202785.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGGCC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT
    GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCTTG
    ACGGGCCTCA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCAGTAAA AGACCAGCAG
    AGGCAGAAGG TTCGCGAGGA GGTGGTTACC GTGGGCAATT CTGTTGACCA GGGCCTGAGT
    CAGCCCACAG ATGACTCCTG CTTCGACCCC TACACGGTCT CCCATTATGC CATTGGAGAG
    GAGTGGGAGC GATTGTCTGA TTCTGGCTTT AAACTCTCGT GCCAGTGCTT AGGCTTTGGC
    AGTGGTCATT TCAGATGCGA TTCATCTAAA TGGTGCCATG ACAATGGAGT GAACTACAAG
    ATTGGAGAGA AGTGGGATCG TCAGGGAGAG AATGGCCAGA TGATGAGCTG CACGTGTCTT
    GGAAATGGAA AAGGAGAATT CAAGTGTGAT CCTCATGAGG CCACGTGCTA TGACGATGGG
    AAGACTTACC ACGTGGGAGA ACAGTGGCAG AAGGAATATC TTGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA
    CCCGGTAACG AAGGCTCCAC TGCCCACTCC TACAACCAGT ATTCCCAGAG ATACCATCAG
    AGAACAAACA CTAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTGGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16678
    Clone ID Related Accession (Same CDS sequence) XM_005202785.3 , XM_005202785.4
    Accession Version XM_005202785.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202785.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGGCC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT
    GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCTTG
    ACGGGCCTCA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCAGTAAA AGACCAGCAG
    AGGCAGAAGG TTCGCGAGGA GGTGGTTACC GTGGGCAATT CTGTTGACCA GGGCCTGAGT
    CAGCCCACAG ATGACTCCTG CTTCGACCCC TACACGGTCT CCCATTATGC CATTGGAGAG
    GAGTGGGAGC GATTGTCTGA TTCTGGCTTT AAACTCTCGT GCCAGTGCTT AGGCTTTGGC
    AGTGGTCATT TCAGATGCGA TTCATCTAAA TGGTGCCATG ACAATGGAGT GAACTACAAG
    ATTGGAGAGA AGTGGGATCG TCAGGGAGAG AATGGCCAGA TGATGAGCTG CACGTGTCTT
    GGAAATGGAA AAGGAGAATT CAAGTGTGAT CCTCATGAGG CCACGTGCTA TGACGATGGG
    AAGACTTACC ACGTGGGAGA ACAGTGGCAG AAGGAATATC TTGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA
    CCCGGTAACG AAGGCTCCAC TGCCCACTCC TACAACCAGT ATTCCCAGAG ATACCATCAG
    AGAACAAACA CTAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTGGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202842.1
    CDS238..7041
    Translation

    Target ORF information:

    RefSeq Version XM_005202785.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202785.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCAACATTG ATCGCCCTAA AGGACTGGCA
    TTCACTGATG TGGATGTCGA TTCCATCAAA ATTGCTTGGG AAAGCCCACA GGGGCAAGTT
    TCCAGGTACA GGGTGACCTA CTCGAGCCCT GAGGATGGAA TCCATGAGCT ATTCCCTGCA
    CCTGATGGTG AAGAAGAGAC TGCAGAGCTG CAAGGCCTCA GGCCGGGTTC TGAGTACACA
    GTCAGTGTGG TTGCCTTGCA CGATGATATG GAGAGCCAGC CCCTGATTGG AACCCAGTCC
    ACAGCCATTC CTGCACCAAC CAACCTGAAG TTTACTCAGG TGACACCAAC CAGCCTGACT
    GCCCAGTGGA CGGCACCCAA TGTTCAGCTC ACTGGGTATC GAGTGCGGGT GACCCCGAAG
    GAGAAGACCG GACCGATGAA AGAAATCAAC CTTGCTCCTG ATAGCTCATC CGTGGTTGTA
    TCAGGACTCA TGGTTGCCAC CAAATATGAA GTGAGTGTCT ATGCTCTTAA GGACACTTTG
    ACAAGCAGAC CGGCTCAGGG AGTCGTTACA ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG
    GCCCGTGTGA CAGATGCTAC TGAGACCACC ATCACCATTA GCTGGAGAAC TAAGACTGAG
    ACGATCACCG GCTTCCAAGT TGATGCTATC CCAGCAAATG GCCAGACCCC AATTCAGAGA
    ACCATCAGGC CAGATGTCAG AAGTTACACC ATCACAGGTT TACAACCTGG TACTGACTAC
    AAGATCCACT TGTACACCTT GAATGACAAT GCCCGGAGCT CCCCTGTGGT CATCGATGCC
    TCCACTGCCA TTGATGCACC ATCCAACCTG CGTTTCCTGG CCACCACACC CAACTCCCTG
    CTGGTATCAT GGCAGCCACC CCGTGCCAGG ATTACTGGTT ACATCATCAA GTACGAGAAG
    CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT CGGCCCCGCC CTGGTGTCAC AGAAGCTACT
    ATCACTGGTC TGGAACCAGG AACAGAGTAC ACAATCCAAG TCATTGCCCT CAAGAACAAT
    CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA AAGACAGGCC AAGAAGCTCT CTCTCAGACA
    ACCATCTCAT GGACCCCATT CCAGGAAAGC TCTGAGTATA TCATTTCATG TCATCCAGTT
    GGCATTGATG AAGAACCCTT ACAGTTCCGA GTTCCTGGAA CCTCTGCTAG TGCCACCTTG
    ACGGGCCTCA CCAGAGGGGC CACCTACAAC ATCATAGTGG AGGCAGTAAA AGACCAGCAG
    AGGCAGAAGG TTCGCGAGGA GGTGGTTACC GTGGGCAATT CTGTTGACCA GGGCCTGAGT
    CAGCCCACAG ATGACTCCTG CTTCGACCCC TACACGGTCT CCCATTATGC CATTGGAGAG
    GAGTGGGAGC GATTGTCTGA TTCTGGCTTT AAACTCTCGT GCCAGTGCTT AGGCTTTGGC
    AGTGGTCATT TCAGATGCGA TTCATCTAAA TGGTGCCATG ACAATGGAGT GAACTACAAG
    ATTGGAGAGA AGTGGGATCG TCAGGGAGAG AATGGCCAGA TGATGAGCTG CACGTGTCTT
    GGAAATGGAA AAGGAGAATT CAAGTGTGAT CCTCATGAGG CCACGTGCTA TGACGATGGG
    AAGACTTACC ACGTGGGAGA ACAGTGGCAG AAGGAATATC TTGGTGCCAT TTGCTCCTGC
    ACATGCTTTG GAGGCCAGCG GGGCTGGCGC TGTGACAACT GCCGCAGACC TGGGGCTGAA
    CCCGGTAACG AAGGCTCCAC TGCCCACTCC TACAACCAGT ATTCCCAGAG ATACCATCAG
    AGAACAAACA CTAATGTCAA CTGCCCAATT GAGTGCTTCA TGCCTTTGGA TGTACAGGCT
    GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16679
    Clone ID Related Accession (Same CDS sequence) XM_005202786.4 , XM_005202786.3
    Accession Version XM_005202786.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product fibronectin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202786.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202843.1
    CDS237..6770
    Translation

    Target ORF information:

    RefSeq Version XM_005202786.4
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202786.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16679
    Clone ID Related Accession (Same CDS sequence) XM_005202786.4 , XM_005202786.3
    Accession Version XM_005202786.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product fibronectin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103848.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202843.1
    CDS234..6767
    Translation

    Target ORF information:

    RefSeq Version XM_005202786.3
    Organism Bos taurus(cattle)
    Definition Bos taurus fibronectin 1 (FN1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202786.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTCGGGG GTCCGGGGCC CGGGCTGCTG CTGCTGCTGG CCGTCCTGTC CCTGGGGACA 
    GCGGTACCCT CCGCGGGAGC CTCGAAGAGC AGGAGACAGG CGCAGCAGAT CGTTCAGCCC
    CAGTCCCCGC TGACTGTCAG TCAGAGCAAA CCTGGTTGTT ATGACAATGG AAAGCACTAT
    CAGATAAACC AACAATGGGA GCGCACCTAC CTGGGCAGCG CCTTGGTCTG TACCTGTTAT
    GGAGGGAGCC GGGGCTTTAA TTGTGAGAGC AAGCCTGAAC CTGAAGAGAC TTGCTTTGAC
    AAGTACACTG GGAACACTTA CCGGGTGGGT GACACTTATG AGCGCCCTAA GGACTCCATG
    ATCTGGGACT GTACCTGCAT CGGCGCTGGG CGAGGAAGAA TAAGCTGCAC CATTGCAAAC
    CGCTGCCATG AAGGGGGGCA GTCCTACAAG ATTGGTGACA CCTGGAGGAG ACCACACGAA
    ACTGGTGGTT ACATGTTAGA GTGTGTGTGT CTGGGTAATG GCAAAGGAGA ATGGACCTGC
    AAGCCCATAG CTGAGAAGTG TTTTGATCAA GCTGCTGGTA CTTCCTACGT TGTCGGGGAG
    ACCTGGGAAA AGCCTTATCA AGGCTGGATG ATGGTGGACT GCACTTGCCT GGGAGAAGGC
    AGCGGACGTA TCACTTGCAC CTCCAGAAAC AGATGCAACG ATCAGGACAC TAGGACATCC
    TATAGAATTG GGGATACCTG GAGCAAGAAG GACAACCGGG GGAACCTGCT CCAGTGCATC
    TGCACGGGCA ATGGCCGTGG CGAGTGGAAG TGTGAGAGGC ATACATCCCT GCAGACCACA
    TCTGCAGGAT CTGGCTCCTT CACTGATGTC CGAACGGCCA TCTACCAGCC CCAGCCTCAT
    CCTCAGCCGC CTCCGTATGG TCACTGTGTC ACAGACAGCG GTGTGGTTTA CTCTGTGGGG
    ATGCAGTGGC TGAAGACACA AGGGAATAAG CAAATGCTTT GCACTTGCCT GGGCAACGGA
    GTCAGCTGCC AAGAGACAGC TGTAACCCAG ACTTATGGTG GCAATTCCAA CGGAGAGCCG
    TGCGTCCTCC CGTTCACCTA CAACGGTAAG ACTTTCTACT CCTGCACGAC CGAAGGGCGG
    CAGGACGGAC ACCTCTGGTG CAGCACAACT TCCAATTATG AGCAAGACCA GAAATATTCT
    TTCTGTACAG ACCATACTGT TTTGGTTCAG ACTCGAGGTG GAAATTCCAA TGGTGCCTTG
    TGCCACTTTC CTTTCCTGTA CAACAACCAC AACTACACGG ACTGTACTTC TGAGGGCAGG
    AGGGACAACA TGAAATGGTG TGGGACCACG CAGAACTATG ACGCTGACCA GAAGTTTGGA
    TTCTGCCCCA TGGCTGCCCA CGAGGAAATC TGTACAACCA ACGAAGGGGT CATGTATCGC
    ATTGGAGACC AGTGGGACAA ACAGCATGAT ATGGGCCACA TGATGAGATG CACATGTGTT
    GGAAATGGTC GTGGAGAATG GACATGTGTC GCCTACTCTC AGCTCCGAGA TCAGTGCATC
    GTCGACGGCA TCACTTACAA TGTGAATGAC ACATTCCACA AACGTCATGA AGAGGGACAC
    ATGCTGAATT GTACCTGCTT CGGTCAGGGC CGGGGTCGGT GGAAGTGCGA TCCTGTTGAT
    CAATGTCAGG ATTCAGAAAC CCGCACCTTT TATCAAATTG GAGACTCATG GGAGAAGTAT
    TTGCAGGGAG TCCGGTACCA GTGCTACTGC TATGGCCGTG GCATTGGGGA GTGGGCCTGC
    CAGCCCTTAC AGACCTATCC AGATACAAGT GGTCCTGTCC AAGTAATCAT CACTGAGACC
    CCAAGTCAAC CCAACTCTCA CCCCATCCAA TGGAGCGCAC CAGAATCATC TCACATTTCC
    AAGTACATTC TCAGATGGAA ACCTAAAAAT TCTCCAAACC GCTGGAAGGA AGCTACCATT
    CCTGGCCACT TAAACTCCTA CACCATCAAA GGCCTGAGGC CAGGTGTGGT ATACGAGGGC
    CAGCTTATCA GCGTCCAGCA CTATGGCCAA AGAGAGGTGA CGCGCTTCGA CTTTACCACC
    ACCAGCACGA GCCCAGCGGT GACCAGCAAC ACTGTGACGG GAGAGACCAC ACCCTTGTCT
    CCTGTTGTGG CCACCTCTGA GTCGGTGACT GAAATCACAG CCAGCAGCTT TGTGGTTTCC
    TGGGTCTCGG CTTCGGACAC TGTGTCTGGA TTCCGGGTGG AGTATGAGCT GAGTGAAGAG
    GGAGATGAAC CACAGTACCT GGATCTTCCC AGCACAGCCA CTTCCGTGAA CATCCCTGAC
    CTGCTTCCCG GCCGAAAATA CACTGTGAAT GTTTACGAGA TATCCGAGGA AGGAGAGCAG
    AATTTGATCC TGTCTACCTC ACAGACCACA GCACCTGACG CCCCTCCTGA CCCTACCGTG
    GACCAAGTTG ATGACACTTC CATTGTCGTT CGCTGGAGCA GACCCAGGGC GCCCATCACA
    GGATACAGAA TCGTCTATTC CCCATCCGTA GAGGGCAGTA GCACAGAACT CAATCTTCCT
    GAAACGGCCA ACTCTGTCAC TCTCAGTGAC TTGCAACCTG GCGTTCAGTA TAACATCACC
    ATCTATGCTG TGGAAGAAAA TCAAGAAAGT ACTCCCGTCT TCATCCAGCA AGAAACCACT
    GGAGTCCCAC GTTCAGATAA AGTTCCCCCT CCCAGGGACC TGCAGTTTGT GGAGGTGACA
    GATGTGAAGA TCACCATCAT GTGGACACCC CCAGAGAGTC CAGTAACCGG TTACCGTGTG
    GATGTGATCC CCGTCAACCT GCCTGGGGAG CATGGGCAGA GGCTGCCCGT CAGCAGGAAC
    ACCTTTGCGG AAGTCACCGG GCTGTCCCCT GGGGTCACTT ATCACTTCAA AGTCTTCGCC
    GTGAACCAGG GGAGGGAGAG CAAGCCTCTG ACGGCACAGC AGGCAACCAA ATTGGATGCT
    CCCACCAACC TCCAGTTTAT CAATGAAACT GATACCACGG TTATAGTGAC CTGGACTCCA
    CCTCGCGCCC GGATAGTTGG GTACCGACTG ACCGTGGGCC TGACCCGGGG AGGCCAGCCC
    AAGCAGTACA ACGTGGGGCC CGCAGCCTCG CAGTACCCAC TGAGGAACCT GCAGCCTGGC
    TCTGAGTATG CTGTAAGCCT CGTAGCCGTG AAGGGCAATC AGCAGAGCCC CAGAGTCACC
    GGAGTCTTCA CCACTCTGCA GCCTCTGGGC TCTATCCCAC ATTACAACAC AGAGGTGACT
    GAGACCACCA TTGTGATTAC ATGGACACCT GCCCCAAGGA TTGGCTTTAA GCTGGGTGTA
    CGACCAAGCC AGGGAGGAGA AGCACCCCGA GAAGTGACTT CAGAGTCAGG AAGCATCGTG
    GTGTCTGGCC TGACTCCAGG TGTGGAATAC GTTTACACCA TCTCTGTCCT CAGAGACGGG
    CAAGAGAGAG ATGCGCCAAT TGTGAAGAAA GTGGTGACAC CACTGTCCCC ACCAACGAAC
    TTGCACCTGG AGGCAAACCC TGACACCGGA GTGCTTACTG TCTCCTGGGA GAGGAGCACC
    ACTCCCGACA TTACTGGCTA CAGAATCACC ACCACCCCAA CAAACGGCCA GCAGGGATAC
    TCTCTGGAGG AAGTGGTCCA TGCCGATCAG AGTTCCTGCA CCTTTGAAAA CCTGAGTCCG
    GGCCTGGAGT ACAATGTCAG TGTTTACACT GTCAAAGATG ACAAGGAAAG TGTCCCTATC
    TCTGATACCA TCATCCCAGC TGTCCCTCCT CCCACTGATT TGCGATTTAC CAATGTTGGT
    CCTGACACAA TGCGTGTCAC CTGGGCTCCA CCCTCATCCA TTGAACTGAC CAACCTCCTG
    GTACGCTACT CGCCTGTGAA AAATGAGGAG GATGTTGCCG AGCTGTCCAT TTCTCCTTCA
    GACAACGCAG TGGTCTTAAC AAATCTCCTG CCCGGCACCG AGTATTTAGT CAGCGTCTCC
    AGTGTTTACG AGCAGCATGA GAGCATACCT CTTAGAGGAA GACAGAAAAC CGCTCTTGAC
    TCCCCAAGTG GCATTGACTT TTCTGATATC ACTGCCAACT CTTTCACTGT CCATTGGATT
    GCTCCACGAG CCACCATCAC TGGCTACAGG ATCCGCCATC ATCCTGAAAA CATGGGTGGA
    AGACCTCGGG AAGATCGAGT GCCCCCCTCT CGGAATTCCA TCACCCTCAC CAACCTGAAC
    CCAGGCACAG AATATGTGGT CAGCATTGTT GCTCTTAATA GCAAAGAGGA AAGTCTGCCC
    TTGGTTGGCC AACAATCAAC AGTTTCTGAT GTTCCAAGGG ACCTGGAAGT CATTGCTGCA
    ACCCCCACTA GCCTGCTGAT CAGCTGGGAT GCGCCTGCCG TCACAGTGAG ATACTACAGG
    ATCACCTATG GAGAAACAGG AGGAAGTAGC CCTGTCCAGG AGTTCACCGT GCCTGGGAGC
    AAGTCTACAG CTACCATCAG TGGTCTTAAA CCTGGAGTGG ACTACACCAT CACTGTCTAT
    GCTGTCACCG GCCGTGGGGA CAGCCCGGCA AGCAGCAAGC CCGTTTCCAT CAATTACCGA
    ACAGAAATTG ACAAACCATC CCAGATGCAA GTGACTGATG TCCAAGACAA CAGCATTAGT
    GTCAGGTGGC TGCCTTCAAG TTCCCCTGTT ACTGGTTACA GAGTGACCAC TGCTCCTAAA
    AATGGCCCAG GACCATCGAA AACGAAAACT GTAGGTCCAG ATCAAACAGA AATGACAATT
    GAAGGCTTGC AGCCCACAGT GGAGTATGTG GTCAGTGTCT ATGCTCAGAA TCAAAACGGA
    GAGAGTCAGC CTCTGGTTCA GACAGCGGTA ACCACCATTC CTGCACCAAC CAACCTGAAG
    TTTACTCAGG TGACACCAAC CAGCCTGACT GCCCAGTGGA CGGCACCCAA TGTTCAGCTC
    ACTGGGTATC GAGTGCGGGT GACCCCGAAG GAGAAGACCG GACCGATGAA AGAAATCAAC
    CTTGCTCCTG ATAGCTCATC CGTGGTTGTA TCAGGACTCA TGGTTGCCAC CAAATATGAA
    GTGAGTGTCT ATGCTCTTAA GGACACTTTG ACAAGCAGAC CGGCTCAGGG AGTCGTTACA
    ACTCTGGAGA ATGTCAGCCC TCCAAGAAGG GCCCGTGTGA CAGATGCTAC TGAGACCACC
    ATCACCATTA GCTGGAGAAC TAAGACTGAG ACGATCACCG GCTTCCAAGT TGATGCTATC
    CCAGCAAATG GCCAGACCCC AATTCAGAGA ACCATCAGGC CAGATGTCAG AAGTTACACC
    ATCACAGGTT TACAACCTGG TACTGACTAC AAGATCCACT TGTACACCTT GAATGACAAT
    GCCCGGAGCT CCCCTGTGGT CATCGATGCC TCCACTGCCA TTGATGCACC ATCCAACCTG
    CGTTTCCTGG CCACCACACC CAACTCCCTG CTGGTATCAT GGCAGCCACC CCGTGCCAGG
    ATTACTGGTT ACATCATCAA GTACGAGAAG CCTGGGTCCC CTCCTAGAGA AGTGGTCCCT
    CGGCCCCGCC CTGGTGTCAC AGAAGCTACT ATCACTGGTC TGGAACCAGG AACAGAGTAC
    ACAATCCAAG TCATTGCCCT CAAGAACAAT CAGAAGAGTG AGCCTTTGAT TGGGAGGAAA
    AAGACAGGCC AAGAAGCTCT CTCTCAGACA ACCATCTCAT GGACCCCATT CCAGGAAAGC
    TCTGAGTATA TCATTTCATG TCATCCAGTT GGCATTGATG AAGAACCCTT ACAGTTCCGA
    GTTCCTGGAA CCTCTGCTAG TGCCACCTTG ACGGGCCTCA CCAGAGGGGC CACCTACAAC
    ATCATAGTGG AGGCAGTAAA AGACCAGCAG AGGCAGAAGG TTCGCGAGGA GGTGGTTACC
    GTGGGCAATT CTGTTGACCA GGGCCTGAGT CAGCCCACAG ATGACTCCTG CTTCGACCCC
    TACACGGTCT CCCATTATGC CATTGGAGAG GAGTGGGAGC GATTGTCTGA TTCTGGCTTT
    AAACTCTCGT GCCAGTGCTT AGGCTTTGGC AGTGGTCATT TCAGATGCGA TTCATCTAAA
    TGGTGCCATG ACAATGGAGT GAACTACAAG ATTGGAGAGA AGTGGGATCG TCAGGGAGAG
    AATGGCCAGA TGATGAGCTG CACGTGTCTT GGAAATGGAA AAGGAGAATT CAAGTGTGAT
    CCTCATGAGG CCACGTGCTA TGACGATGGG AAGACTTACC ACGTGGGAGA ACAGTGGCAG
    AAGGAATATC TTGGTGCCAT TTGCTCCTGC ACATGCTTTG GAGGCCAGCG GGGCTGGCGC
    TGTGACAACT GCCGCAGACC TGGGGCTGAA CCCGGTAACG AAGGCTCCAC TGCCCACTCC
    TACAACCAGT ATTCCCAGAG ATACCATCAG AGAACAAACA CTAATGTCAA CTGCCCAATT
    GAGTGCTTCA TGCCTTTGGA TGTACAGGCT GACAGAGAAG ATTCCCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.