gRNAs
The following F10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa44395 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001080213.1 , NM_001080213.2 | |
| Accession Version | NM_001080213.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-01-03 | |
| Organism | Bos taurus(cattle) | |
| Product | coagulation factor X precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC150564.5. On Oct 25, 2009 this sequence version replaced XM_613822.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X00673.1, BC104607.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02404548, SAMN02404549 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCGGGCC TGCTGCATCT CGTTCTGCTC AGCACCGCCC TGGGCGGCCT CCTGCGGCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001073682.1 |
| CDS | 76..1554 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001080213.1 |
1 | ATGGCGGGCC TGCTGCATCT CGTTCTGCTC AGCACCGCCC TGGGCGGCCT CCTGCGGCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa44395 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001080213.1 , NM_001080213.2 | |
| Accession Version | NM_001080213.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-23 | |
| Organism | Bos taurus(cattle) | |
| Product | coagulation factor X precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000012.1. On May 29, 2018 this sequence version replaced NM_001080213.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.14101.1, SRR5571260.16628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145414, SAMN03145416 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCGGGCC TGCTGCATCT CGTTCTGCTC AGCACCGCCC TGGGCGGCCT CCTGCGGCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001073682.1 |
| CDS | 76..1554 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001080213.2 |
1 | ATGGCGGGCC TGCTGCATCT CGTTCTGCTC AGCACCGCCC TGGGCGGCCT CCTGCGGCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa45012 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024999779.1 | |
| Accession Version | XM_024999779.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | coagulation factor X isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGACATG ACTTTTATCA TGGTCGTAAA GATGAGAACA GAGCTGGATG TGGACGTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024855547.1 |
| CDS | 106..1932 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024999779.1 |
1 | ATGAGACATG ACTTTTATCA TGGTCGTAAA GATGAGAACA GAGCTGGATG TGGACGTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Lipid specificity of the membrane binding domain of coagulation factor X. |
Factor Xa dimerization competes with prothrombinase complex formation on platelet-like membrane surfaces. |
A whole-genome assembly of the domestic cow, Bos taurus. |
Factor Xa active site substrate specificity with substrate phage display and computational molecular modeling. |
Linear diffusion of thrombin and factor Xa along the heparin molecule explains the effects of extended heparin chain lengths. |
