CA2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CA2
Entrez Gene ID	280740
Full Name	carbonic anhydrase II
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_178572.2	NP_848667.1	carbonic anhydrase 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Car2	NP_062164.1
	CA2	XP_001096360.1
Pan troglodytes (chimpanzee)	CA2	NP_001181853.1
Bos taurus (cattle)	CA2	NP_848667.1
Gallus gallus (chicken)	CA2	NP_990648.1
Mus musculus (house mouse)	Car2	NP_033931.4
Homo sapiens (human)	CA2	NP_000058.1
Canis lupus familiaris (dog)	CA2	NP_001138642.1
Xenopus tropicalis (tropical clawed frog)	ca2	NP_001015729.1

Gene Ontology
Bibliography

Related articles in PubMed

Carbonic anhydrases are producers of S-nitrosothiols from inorganic nitrite and modulators of soluble guanylyl cyclase in human platelets.
Hanff E, B?hmer A, Zinke M, Gambaryan S, Schwarz A, Supuran CT, Tsikas D
Amino acids48(7)1695-706(2016 Jul)

Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst.
Warden AC, Williams M, Peat TS, Seabrook SA, Newman J, Dojchinov G, Haritos VS
Nature communications610278(2015 Dec)

Spectroscopic and molecular modeling studies on binding of dorzolamide to bovine and human carbonic anhydrase II.
Bijari N, Ghobadi S, Mahdiuni H, Khodarahmi R, Ghadami SA
International journal of biological macromolecules80189-99(2015 Sep)

Ion mobility and collision-induced dissociation analysis of carbonic anhydrase 2.
Nabuchi Y, Hirose K, Takayama M
Analytical chemistry82(21)8890-6(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provides the first evidence that extra-platelet nitrite and erythrocytic CAII may modulate platelet function in a cGMP-dependent manner.
Title: Carbonic anhydrases are producers of S-nitrosothiols from inorganic nitrite and modulators of soluble guanylyl cyclase in human platelets.

Increasing the number and distribution of surface acidic groups of CAII is sufficient to function in high-salt environments.
Title: Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst.

binding of dorzolamide to bovine and human carbonic anhydrase II
Title: Spectroscopic and molecular modeling studies on binding of dorzolamide to bovine and human carbonic anhydrase II.

Folding analysis of the zinc protein, carbonic anhydrase 2 was performed using electrospray ionization ion mobility spectrometry coupled with collision-induced dissociation.
Title: Ion mobility and collision-induced dissociation analysis of carbonic anhydrase 2.

Intense positive staining for CA-II was found in luminal duct cells and for the first time also inside the duct lumen, clearly demonstrating the expression and secretion of salivary CA-II in bovine parotid glands.
Title: Carbonic anhydrase II is secreted from bovine parotid glands.

The following CA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01330	NM_178572.2 Latest version!	Bos taurus carbonic anhydrase II (CA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CA2 ( NM_178572.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_848667.1 Bos taurus carbonic anhydrase II (CA2), mRNA.

Price & Availability↑

CloneID	OBa01330
Clone ID Related Accession (Same CDS sequence)	NM_178572.2
Accession Version	NM_178572.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	carbonic anhydrase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY240020.1. On Jun 3, 2003 this sequence version replaced NM_178572.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY240020.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCCCATC ACTGGGGATA CGGCAAGCAC AACGGCCCCG AACACTGGCA TAAGGACTTT 
CCCATTGCCA ACGGAGAGCG CCAGTCACCC GTTGACATCG ACACCAAGGC CGTCGTTCAG 
GATCCTGCTT TGAAGCCTCT GGCCCTTGTC TATGGGGAAG CAACTTCGCG GAGAATGGTC 
AACAATGGTC ACTCTTTTAA CGTGGAGTAT GACGACTCCC AGGACAAAGC AGTGCTGAAA 
GATGGACCTC TTACTGGCAC TTACAGATTG GTTCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGACCAAG GTTCTGAGCA TACTGTGGAT AGGAAGAAAT ATGCTGCCGA GCTACACCTG 
GTTCACTGGA ACACCAAGTA CGGGGACTTT GGAACAGCAG CACAGCAGCC TGATGGACTG 
GCTGTTGTGG GTGTTTTTTT GAAGGTTGGT GATGCTAACC CAGCCCTCCA GAAAGTCCTT 
GACGCGCTGG ATTCTATTAA AACAAAGGGT AAGAGCACCG ACTTCCCCAA CTTCGATCCG 
GGCAGCCTCC TTCCTAACGT CTTGGACTAC TGGACCTACC CTGGCTCACT GACCACCCCT 
CCTCTCCTCG AAAGCGTGAC CTGGATCGTG CTCAAAGAAC CCATCTCTGT TAGCAGTCAG 
CAGATGTTGA AATTCCGTAC TCTTAACTTC AACGCGGAGG GCGAGCCTGA ACTCCTGATG 
TTGGCCAACT GGCGTCCGGC TCAGCCGCTG AAAAACAGAC AAGTCAGAGG TTTCCCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848667.1
CDS	50..832
Translation	MSHHWGYGKHNGPEHWHKDFPIANGERQSPVDIDTKAVVQDPALKPLALVYGEATSRRMVNNGHSFNVEYDDSQDKAVLKDGPLTGTYRLVQFHFHWGSSDDQGSEHTVDRKKYAAELHLVHWNTKYGDFGTAAQQPDGLAVVGVFLKVGDANPALQKVLDALDSIKTKGKSTDFPNFDPGSLLPNVLDYWTYPGSLTTPPLLESVTWIVLKEPISVSSQQMLKFRTLNFNAEGEPELLMLANWRPAQPLKNRQVRGFPK

Target ORF information:

RefSeq Version	NM_178572.2
Organism	Bos taurus(cattle)
Definition	Bos taurus carbonic anhydrase II (CA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178572.2

ORF Insert Sequence:

ATGTCCCATC ACTGGGGATA CGGCAAGCAC AACGGCCCCG AACACTGGCA TAAGGACTTT 
CCCATTGCCA ACGGAGAGCG CCAGTCACCC GTTGACATCG ACACCAAGGC CGTCGTTCAG 
GATCCTGCTT TGAAGCCTCT GGCCCTTGTC TATGGGGAAG CAACTTCGCG GAGAATGGTC 
AACAATGGTC ACTCTTTTAA CGTGGAGTAT GACGACTCCC AGGACAAAGC AGTGCTGAAA 
GATGGACCTC TTACTGGCAC TTACAGATTG GTTCAGTTTC ACTTTCACTG GGGCTCATCT 
GATGACCAAG GTTCTGAGCA TACTGTGGAT AGGAAGAAAT ATGCTGCCGA GCTACACCTG 
GTTCACTGGA ACACCAAGTA CGGGGACTTT GGAACAGCAG CACAGCAGCC TGATGGACTG 
GCTGTTGTGG GTGTTTTTTT GAAGGTTGGT GATGCTAACC CAGCCCTCCA GAAAGTCCTT 
GACGCGCTGG ATTCTATTAA AACAAAGGGT AAGAGCACCG ACTTCCCCAA CTTCGATCCG 
GGCAGCCTCC TTCCTAACGT CTTGGACTAC TGGACCTACC CTGGCTCACT GACCACCCCT 
CCTCTCCTCG AAAGCGTGAC CTGGATCGTG CTCAAAGAAC CCATCTCTGT TAGCAGTCAG 
CAGATGTTGA AATTCCGTAC TCTTAACTTC AACGCGGAGG GCGAGCCTGA ACTCCTGATG 
TTGGCCAACT GGCGTCCGGC TCAGCCGCTG AAAAACAGAC AAGTCAGAGG TTTCCCCAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Carbonic anhydrases are producers of S-nitrosothiols from inorganic nitrite and modulators of soluble guanylyl cyclase in human platelets.
Amino acids48(7)1695-706(2016 Jul)
Hanff E,B?hmer A,Zinke M,Gambaryan S,Schwarz A,Supuran CT,Tsikas D

Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst.
Nature communications610278(2015 Dec)
Warden AC,Williams M,Peat TS,Seabrook SA,Newman J,Dojchinov G,Haritos VS

Spectroscopic and molecular modeling studies on binding of dorzolamide to bovine and human carbonic anhydrase II.
International journal of biological macromolecules80189-99(2015 Sep)
Bijari N,Ghobadi S,Mahdiuni H,Khodarahmi R,Ghadami SA

Ion mobility and collision-induced dissociation analysis of carbonic anhydrase 2.
Analytical chemistry82(21)8890-6(2010 Nov)
Nabuchi Y,Hirose K,Takayama M

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CA2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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